Brucella ovis

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D.m. West - One of the best experts on this subject based on the ideXlab platform.

  • seroconversion and semen shedding in rams experimentally infected with Brucella ovis
    New Zealand Veterinary Journal, 2014
    Co-Authors: A L Ridler, Sl Smith, D.m. West
    Abstract:

    AIM: To determine the time taken for rams to develop antibodies to Brucella ovis in serum, shed B. ovis in semen and develop lesions of epididymitis following infection with B. ovis.METHODS: Fifteen 19-month-old rams were artificially infected with B. ovis by inoculation of infected semen onto the nasal and rectal mucus membranes (Day 0). Serum was collected from each ram at 2 to 8-day intervals and tested at commercial laboratories using a complement fixation test (CFT) and an ELISA. Cut-off values for the CFT were 0–4/4 negative; 1/8–3/8 suspicious and 4/8–4/128 positive, and for the ELISA were <10% negative; ≥10 to <50% suspicious and ≥50% positive. Selected serum samples were also tested using a gel diffusion test (GDT). At 7 to 8-day intervals semen was collected for bacterial culture and the scrotal contents were palpated to identify lesions of epididymitis. The study was terminated after 56 days.RESULTS: On Day 28 B. ovis was isolated from the semen of one ram and by Day 49 it was isolated from the...

  • Seroconversion and semen shedding in rams experimentally infected with Brucella ovis
    New Zealand Veterinary Journal, 2013
    Co-Authors: Anne Ridler, Sl Smith, D.m. West
    Abstract:

    AIM: To determine the time taken for rams to develop antibodies to Brucella ovis in serum, shed B. ovis in semen and develop lesions of epididymitis following infection with B. ovis.METHODS: Fifteen 19-month-old rams were artificially infected with B. ovis by inoculation of infected semen onto the nasal and rectal mucus membranes (Day 0). Serum was collected from each ram at 2 to 8-day intervals and tested at commercial laboratories using a complement fixation test (CFT) and an ELISA. Cut-off values for the CFT were 0–4/4 negative; 1/8–3/8 suspicious and 4/8–4/128 positive, and for the ELISA were

  • Pathology of Brucella ovis infection in red deer stags (Cervus elaphus).
    New Zealand Veterinary Journal, 2012
    Co-Authors: Anne Ridler, D.m. West, Mark G. Collett
    Abstract:

    Abstract AIM: To describe the pathology of the reproductive tract of red deer stags with active Brucella ovis infection and in stags in which B. ovis infection had resolved. METHODS: Twenty-three red deer stags of varying history were slaughtered and their epididymides and accessory sex glands examined grossly and by histopathology. At the time of slaughter five of the stags had an active B. ovis infection of 24–55 days duration following exposure to infected rams, 10 stags had been experimentally infected with B. ovis by intravenous inoculation 649 days previously and had developed an active infection but the bacterial infection had resolved at least 308 days prior to slaughter, and eight stags had not been exposed to B. ovis at any time. RESULTS: Of the five stags with an active infection, one had gross enlargement of the epididymides that could be detected by scrotal palpation. Histological lesions in all five stags included mild to severe, predominantly non-suppurative epididymitis, vesiculitis, prost...

  • Control of Brucella ovis infection in sheep.
    Veterinary Clinics of North America: Food Animal Practice, 2011
    Co-Authors: Anne Ridler, D.m. West
    Abstract:

    Approach to control of Brucella ovis would vary in different countries and areas depending on farm and flock characteristics and economic factors. Eradication by a test-and-slaughter approach is the most desirable option in areas where it is logistically and financially feasible. Vaccination is used in areas with a high incidence of infection where eradication would be difficult. Voluntary accreditation programs have been established in some countries and are of particular benefit to pedigree ram breeders.

  • transmission of Brucella ovis from rams to red deer stags
    New Zealand Veterinary Journal, 2000
    Co-Authors: A L Ridler, D.m. West, K J Stafford, P R Wilson, S G Fenwick
    Abstract:

    Abstract Aim: To determine whether B. ovis will transmit from infected rams to non-infected red deer stags (Cervus elaphus) grazing together in the same paddock. Methods: Six rams artificially infected with B. ovis were grazed with six non-infected 14-month-old red deer stags for a four and a half month period from March 4 to July 20, 1999. Stags were blood sampled at one- to six- weekly intervals to test for B. ovis antibodies using a complement fixation test. Stags that seroconverted were semen sampled to test for B. ovis infection by bacteriological culture. Results: Between day 92 and day 124 of grazing together (June 4 and July 6), sera from five of the six stags became positive in the B. ovis complement fixation test. B. ovis was cultured from semen samples from four of the seropositive stags. Conclusions: Brucella ovis can be transmitted from infected rams to non-infected stags grazing in the same paddock, suggesting that B. ovis infection in farmed deer in New Zealand initially came from infected ...

Anne Ridler - One of the best experts on this subject based on the ideXlab platform.

  • Seroconversion and semen shedding in rams experimentally infected with Brucella ovis
    New Zealand Veterinary Journal, 2013
    Co-Authors: Anne Ridler, Sl Smith, D.m. West
    Abstract:

    AIM: To determine the time taken for rams to develop antibodies to Brucella ovis in serum, shed B. ovis in semen and develop lesions of epididymitis following infection with B. ovis.METHODS: Fifteen 19-month-old rams were artificially infected with B. ovis by inoculation of infected semen onto the nasal and rectal mucus membranes (Day 0). Serum was collected from each ram at 2 to 8-day intervals and tested at commercial laboratories using a complement fixation test (CFT) and an ELISA. Cut-off values for the CFT were 0–4/4 negative; 1/8–3/8 suspicious and 4/8–4/128 positive, and for the ELISA were

  • Pathology of Brucella ovis infection in red deer stags (Cervus elaphus).
    New Zealand Veterinary Journal, 2012
    Co-Authors: Anne Ridler, D.m. West, Mark G. Collett
    Abstract:

    Abstract AIM: To describe the pathology of the reproductive tract of red deer stags with active Brucella ovis infection and in stags in which B. ovis infection had resolved. METHODS: Twenty-three red deer stags of varying history were slaughtered and their epididymides and accessory sex glands examined grossly and by histopathology. At the time of slaughter five of the stags had an active B. ovis infection of 24–55 days duration following exposure to infected rams, 10 stags had been experimentally infected with B. ovis by intravenous inoculation 649 days previously and had developed an active infection but the bacterial infection had resolved at least 308 days prior to slaughter, and eight stags had not been exposed to B. ovis at any time. RESULTS: Of the five stags with an active infection, one had gross enlargement of the epididymides that could be detected by scrotal palpation. Histological lesions in all five stags included mild to severe, predominantly non-suppurative epididymitis, vesiculitis, prost...

  • Control of Brucella ovis infection in sheep.
    Veterinary Clinics of North America: Food Animal Practice, 2011
    Co-Authors: Anne Ridler, D.m. West
    Abstract:

    Approach to control of Brucella ovis would vary in different countries and areas depending on farm and flock characteristics and economic factors. Eradication by a test-and-slaughter approach is the most desirable option in areas where it is logistically and financially feasible. Vaccination is used in areas with a high incidence of infection where eradication would be difficult. Voluntary accreditation programs have been established in some countries and are of particular benefit to pedigree ram breeders.

  • An overview of Brucella ovis infection in New Zealand.
    New Zealand Veterinary Journal, 2002
    Co-Authors: Anne Ridler
    Abstract:

    Abstract Extract Brucella ovis is a bacterial disease that causes epididymitis and reduced fertility of rams. It is an important cause of ram wastage in sheep flocks in most parts of the world with the exception of Great Britain (Lawrence 1961). The earliest report of disease caused by the organism now known as B. ovis was from the Gisborne region of New Zealand, where abortion in ewes and epididymitis in rams was documented (McFarlane et al 1952). The following year, reports emerged from New Zealand and Australia of a Brucella-like organism or a Brucella mutant causing epididymitis in rams (Buddie and Boyes 1953; Simmons and Hall 1953). The organism was characterised at the Wallaceville Animal Research Station, Upper Hutt, New Zealand, and named Brucella ovis (Buddie 1956).

Hujang Lee - One of the best experts on this subject based on the ideXlab platform.

  • bactericidal efficacy of vital oxide disinfectant solution against salmonella typhimurium and Brucella ovis
    Journal of Food Hygiene and Safety, 2012
    Co-Authors: Chunnam Cha, Yeoeun Lee, Injin Kang, Changyeul Yoo, Suk Kim, Hujang Lee
    Abstract:

    Salmonella spp. and Brucella spp. have caused a considerable disease of farmed animals and eco- nomic loss in animal farming and food industry. In this study, the disinfection efficacy of Vital-Oxidel ® , a commercial disinfectant, composed to chlorine dioxide, betaine hydrochloride, and propylene glycol was evaluated against S. typhimurium and Brucella ovis. A bactericidal efficacy test by broth dilution method was used to determine the lowest effective dilution of the disinfectant following exposure to test bacteria for 30 min at 4 o C. Vital-Oixdel ® and test bac- teria were diluted with distilled water (DW), hard water (HW) or organic matter suspension (OM) according to treat- ment condition. On OM condition, the bactericidal activity of Vital-Oixdel ® against S. typhimurium and Brucella ovis was lowered compared to that on HW condition. As Vital-Oxidel ® possesses bactericidal efficacy against animal

  • Bactericidal Efficacy of Vital-Oxide ® , Disinfectant Solution Against Salmonella Typhimurium and Brucella ovis
    Journal of Food Hygiene and Safety, 2012
    Co-Authors: Chunnam Cha, Yeoeun Lee, Injin Kang, Changyeul Yoo, Suk Kim, Hujang Lee
    Abstract:

    Salmonella spp. and Brucella spp. have caused a considerable disease of farmed animals and eco- nomic loss in animal farming and food industry. In this study, the disinfection efficacy of Vital-Oxidel ® , a commercial disinfectant, composed to chlorine dioxide, betaine hydrochloride, and propylene glycol was evaluated against S. typhimurium and Brucella ovis. A bactericidal efficacy test by broth dilution method was used to determine the lowest effective dilution of the disinfectant following exposure to test bacteria for 30 min at 4 o C. Vital-Oixdel ® and test bac- teria were diluted with distilled water (DW), hard water (HW) or organic matter suspension (OM) according to treat- ment condition. On OM condition, the bactericidal activity of Vital-Oixdel ® against S. typhimurium and Brucella ovis was lowered compared to that on HW condition. As Vital-Oxidel ® possesses bactericidal efficacy against animal

A L Ridler - One of the best experts on this subject based on the ideXlab platform.

  • seroconversion and semen shedding in rams experimentally infected with Brucella ovis
    New Zealand Veterinary Journal, 2014
    Co-Authors: A L Ridler, Sl Smith, D.m. West
    Abstract:

    AIM: To determine the time taken for rams to develop antibodies to Brucella ovis in serum, shed B. ovis in semen and develop lesions of epididymitis following infection with B. ovis.METHODS: Fifteen 19-month-old rams were artificially infected with B. ovis by inoculation of infected semen onto the nasal and rectal mucus membranes (Day 0). Serum was collected from each ram at 2 to 8-day intervals and tested at commercial laboratories using a complement fixation test (CFT) and an ELISA. Cut-off values for the CFT were 0–4/4 negative; 1/8–3/8 suspicious and 4/8–4/128 positive, and for the ELISA were <10% negative; ≥10 to <50% suspicious and ≥50% positive. Selected serum samples were also tested using a gel diffusion test (GDT). At 7 to 8-day intervals semen was collected for bacterial culture and the scrotal contents were palpated to identify lesions of epididymitis. The study was terminated after 56 days.RESULTS: On Day 28 B. ovis was isolated from the semen of one ram and by Day 49 it was isolated from the...

  • transmission of Brucella ovis from rams to red deer stags
    New Zealand Veterinary Journal, 2000
    Co-Authors: A L Ridler, D.m. West, K J Stafford, P R Wilson, S G Fenwick
    Abstract:

    Abstract Aim: To determine whether B. ovis will transmit from infected rams to non-infected red deer stags (Cervus elaphus) grazing together in the same paddock. Methods: Six rams artificially infected with B. ovis were grazed with six non-infected 14-month-old red deer stags for a four and a half month period from March 4 to July 20, 1999. Stags were blood sampled at one- to six- weekly intervals to test for B. ovis antibodies using a complement fixation test. Stags that seroconverted were semen sampled to test for B. ovis infection by bacteriological culture. Results: Between day 92 and day 124 of grazing together (June 4 and July 6), sera from five of the six stags became positive in the B. ovis complement fixation test. B. ovis was cultured from semen samples from four of the seropositive stags. Conclusions: Brucella ovis can be transmitted from infected rams to non-infected stags grazing in the same paddock, suggesting that B. ovis infection in farmed deer in New Zealand initially came from infected ...

Chunnam Cha - One of the best experts on this subject based on the ideXlab platform.

  • bactericidal efficacy of vital oxide disinfectant solution against salmonella typhimurium and Brucella ovis
    Journal of Food Hygiene and Safety, 2012
    Co-Authors: Chunnam Cha, Yeoeun Lee, Injin Kang, Changyeul Yoo, Suk Kim, Hujang Lee
    Abstract:

    Salmonella spp. and Brucella spp. have caused a considerable disease of farmed animals and eco- nomic loss in animal farming and food industry. In this study, the disinfection efficacy of Vital-Oxidel ® , a commercial disinfectant, composed to chlorine dioxide, betaine hydrochloride, and propylene glycol was evaluated against S. typhimurium and Brucella ovis. A bactericidal efficacy test by broth dilution method was used to determine the lowest effective dilution of the disinfectant following exposure to test bacteria for 30 min at 4 o C. Vital-Oixdel ® and test bac- teria were diluted with distilled water (DW), hard water (HW) or organic matter suspension (OM) according to treat- ment condition. On OM condition, the bactericidal activity of Vital-Oixdel ® against S. typhimurium and Brucella ovis was lowered compared to that on HW condition. As Vital-Oxidel ® possesses bactericidal efficacy against animal

  • Bactericidal Efficacy of Vital-Oxide ® , Disinfectant Solution Against Salmonella Typhimurium and Brucella ovis
    Journal of Food Hygiene and Safety, 2012
    Co-Authors: Chunnam Cha, Yeoeun Lee, Injin Kang, Changyeul Yoo, Suk Kim, Hujang Lee
    Abstract:

    Salmonella spp. and Brucella spp. have caused a considerable disease of farmed animals and eco- nomic loss in animal farming and food industry. In this study, the disinfection efficacy of Vital-Oxidel ® , a commercial disinfectant, composed to chlorine dioxide, betaine hydrochloride, and propylene glycol was evaluated against S. typhimurium and Brucella ovis. A bactericidal efficacy test by broth dilution method was used to determine the lowest effective dilution of the disinfectant following exposure to test bacteria for 30 min at 4 o C. Vital-Oixdel ® and test bac- teria were diluted with distilled water (DW), hard water (HW) or organic matter suspension (OM) according to treat- ment condition. On OM condition, the bactericidal activity of Vital-Oixdel ® against S. typhimurium and Brucella ovis was lowered compared to that on HW condition. As Vital-Oxidel ® possesses bactericidal efficacy against animal