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Peter Vandamme - One of the best experts on this subject based on the ideXlab platform.
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Phylogenomic Study of Burkholderia glathei-like Organisms, Proposal of 13 Novel Burkholderia Species and Emended Descriptions of Burkholderia sordidicola, Burkholderia zhejiangensis, and Burkholderia grimmiae
Frontiers in microbiology, 2016Co-Authors: Charlotte Peeters, Jan P. Meier-kolthoff, Bart Verheyde, Evie De Brandt, Vaughn S. Cooper, Peter VandammeAbstract:Partial gyrB gene sequence analysis of 17 isolates from human and environmental sources revealed 13 clusters of strains and identified them as Burkholderia glathei clade (BGC) bacteria. The taxonomic status of these clusters was examined by whole-genome sequence analysis, determination of the G+C content, whole-cell fatty acid analysis and biochemical characterization. The whole-genome sequence-based phylogeny was assessed using the Genome Blast Distance Phylogeny (GBDP) method and an extended multilocus sequence analysis (MLSA) approach. The results demonstrated that these 17 BGC isolates represented 13 novel Burkholderia species that could be distinguished by both genotypic and phenotypic characteristics. BGC strains exhibited a broad metabolic versatility and developed beneficial, symbiotic, and pathogenic interactions with different hosts. Our data also confirmed that there is no phylogenetic subdivision in the genus Burkholderia that distinguishes beneficial from pathogenic strains. We therefore propose to formally classify the 13 novel BGC Burkholderia species as Burkholderia arvi sp. nov. (type strain LMG 29317(T) = CCUG 68412(T)), Burkholderia hypogeia sp. nov. (type strain LMG 29322(T) = CCUG 68407(T)), Burkholderia ptereochthonis sp. nov. (type strain LMG 29326(T) = CCUG 68403(T)), Burkholderia glebae sp. nov. (type strain LMG 29325(T) = CCUG 68404(T)), Burkholderia pedi sp. nov. (type strain LMG 29323(T) = CCUG 68406(T)), Burkholderia arationis sp. nov. (type strain LMG 29324(T) = CCUG 68405(T)), Burkholderia fortuita sp. nov. (type strain LMG 29320(T) = CCUG 68409(T)), Burkholderia temeraria sp. nov. (type strain LMG 29319(T) = CCUG 68410(T)), Burkholderia calidae sp. nov. (type strain LMG 29321(T) = CCUG 68408(T)), Burkholderia concitans sp. nov. (type strain LMG 29315(T) = CCUG 68414(T)), Burkholderia turbans sp. nov. (type strain LMG 29316(T) = CCUG 68413(T)), Burkholderia catudaia sp. nov. (type strain LMG 29318(T) = CCUG 68411(T)) and Burkholderia peredens sp. nov. (type strain LMG 29314(T) = CCUG 68415(T)). Furthermore, we present emended descriptions of the species Burkholderia sordidicola, Burkholderia zhejiangensis and Burkholderia grimmiae. The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA and gyrB gene sequences determined in this study are LT158612-LT158624 and LT158625-LT158641, respectively.
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Burkholderia: an update on taxonomy and biotechnological potential as antibiotic producers
Applied Microbiology and Biotechnology, 2016Co-Authors: Eliza Depoorter, Tom Coenye, Peter Vandamme, Charlotte Peeters, Matt J. Bull, Eshwar MahenthiralingamAbstract:Burkholderia is an incredibly diverse and versatile Gram-negative genus, within which over 80 species have been formally named and multiple other genotypic groups likely represent new species. Phylogenetic analysis based on the 16S rRNA gene sequence and core genome ribosomal multilocus sequence typing analysis indicates the presence of at least three major clades within the genus. Biotechnologically, Burkholderia are well-known for their bioremediation and biopesticidal properties. Within this review, we explore the ability of Burkholderia to synthesise a wide range of antimicrobial compounds ranging from historically characterised antifungals to recently described antibacterial antibiotics with activity against multiresistant clinical pathogens. The production of multiple Burkholderia antibiotics is controlled by quorum sensing and examples of quorum sensing pathways found across the genus are discussed. The capacity for antibiotic biosynthesis and secondary metabolism encoded within Burkholderia genomes is also evaluated. Overall, Burkholderia demonstrate significant biotechnological potential as a source of novel antibiotics and bioactive secondary metabolites.
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Members of the genus Burkholderia: good and bad guys.
F1000Research, 2016Co-Authors: Leo Eberl, Peter VandammeAbstract:In the 1990s several biocontrol agents on that contained Burkholderia strains were registered by the United States Environmental Protection Agency (EPA). After risk assessment these products were withdrawn from the market and a moratorium was placed on the registration of Burkholderia-containing products, as these strains may pose a risk to human health. However, over the past few years the number of novel Burkholderia species that exhibit plant-beneficial properties and are normally not isolated from infected patients has increased tremendously. In this commentary we wish to summarize recent efforts that aim at discerning pathogenic from beneficial Burkholderia strains.
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Burkholderia humi sp. nov., Burkholderia choica sp. nov., Burkholderia telluris sp. nov., Burkholderia terrestris sp. nov. and Burkholderia udeis sp. nov.: Burkholderia glathei-like bacteria from soil and rhizosphere soil.
International Journal of Systematic and Evolutionary Microbiology, 2013Co-Authors: Peter Vandamme, Evie De Brandt, Kurt Houf, Joana Falcão Salles, Jan Dirk Van Elsas, Theodore Spilker, John J. LipumaAbstract:Analysis of partial gyrB gene sequences revealed six taxa in a group of 17 Burkholderia glathei-like isolates which were further examined by (GTG)(5)-PCR fingerprinting, 16S rRNA gene sequence analysis, DNA-DNA hybridizations, determination of the DNA G+C content, whole-cell fatty acid analysis and an analysis of cell and colony morphology and more than 180 biochemical characteristics. The results demonstrated that one taxon consisting of three human clinical isolates represented Burkholderia zhejiangensis, a recently described methyl-parathion-degrading bacterium isolated from a wastewater-treatment system in China. The remaining taxa represented five novel species isolated from soil or rhizosphere soil samples, and could be distinguished by both genotypic and phenotypic characteristics. We therefore propose to formally classify these bacteria as Burkholderia humi sp. nov. (type strain, LMG 22934(T)=CCUG 63059(T)), Burkholderia choica sp. nov. (type strain, LMG 22940(T)=CCUG 63063(T)), Burkholderia telluris sp. nov. (type strain, LMG 22936(T)=CCUG 63060(T)), Burkholderia udeis sp. nov. (type strain, LMG 27134(T)=CCUG 63061(T)) and Burkholderia terrestris sp. nov. (type strain, LMG 22937(T)=CCUG 63062(T)).
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Burkholderia latens sp nov., Burkholderia diffusa sp nov., Burkholderia arboris sp nov., Burkholderia seminalis sp nov and Burkholderia metallica sp nov., novel species within the Burkholderia cepacia complex
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2008Co-Authors: Elke Vanlaere, Deborah A Henry, David P. Speert, Eshwar Mahenthiralingam, John J. Lipuma, Evie De Brandt, Adam Baldwin, Christopher G. Dowson, Peter VandammeAbstract:The taxonomic position of five recA gene clusters of Burkholderia cepacia complex (Bcc) isolates was determined using a polyphasic taxonomic approach. The levels of 16S rRNA and recA gene sequence similarity, multilocus sequence typing (MLST) data and the intermediate DNA–DNA binding values demonstrated that these five clusters represented five novel species within the Bcc. Biochemical identification of these species is difficult, as is the case for most Bcc species. However, identification of these novel species can be accomplished through recA gene sequence analysis, MLST and BOX-PCR profiling and by recA RFLP analysis. For diagnostic laboratories, recA gene sequence analysis offers the best combination of accuracy and simplicity. Based on these results, we propose five novel Bcc species, Burkholderia latens sp. nov. (type strain FIRENZE 3T =LMG 24064T =CCUG 54555T), Burkholderia diffusa sp. nov. (type strain AU1075T =LMG 24065T =CCUG 54558T), Burkholderia arboris sp. nov. (type strain ES0263AT =LMG 24066T =CCUG 54561T), Burkholderia seminalis sp. nov. (type strain AU0475T =LMG 24067T =CCUG 54564T) and Burkholderia metallica sp. nov. (type strain AU0553T =LMG 24068T =CCUG 54567T). In the present study, we also demonstrate that Burkholderia ubonensis should be considered a member of the Bcc.
Tom Coenye - One of the best experts on this subject based on the ideXlab platform.
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Antimicrobial Drug Efflux Pumps in Burkholderia
Efflux-Mediated Antimicrobial Resistance in Bacteria, 2016Co-Authors: Viola Camilla Scoffone, Tom Coenye, Giovanna Riccardi, Silvia BuroniAbstract:The genus Burkholderia includes more than 90 species able to colonize different environments and characterized by a versatile metabolism. Some members of the Burkholderia genus are opportunistic pathogens, especially for immunocompromised and cystic fibrosis patients. Of note, they show a high level of intrinsic drug resistance, and many genes encoding virulence factors were identified in their genomes. Main contributors to antimicrobial resistance of these bacteria are efflux pump proteins which span the cytoplasmic and outer membranes. These systems are able to recognize and extrude very dissimilar compounds, thus rendering the antimicrobial therapy challenging. A detailed description of the resistance-nodulation-cell division (RND) transporter superfamily, which is the most represented in Gram-negative bacteria such as Burkholderia spp., is given. This includes the distribution of RND-encoding genes in the various Burkholderia spp. genomes and the list of the principal RND pumps in B. cenocepacia, B. vietnamiensis, B. pseudomallei, B. mallei and B. thailandensis. The clinical significance of RND efflux transporters in Burkholderia spp. and relevant existing efflux pump inhibitors is also discussed.
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Burkholderia: an update on taxonomy and biotechnological potential as antibiotic producers
Applied Microbiology and Biotechnology, 2016Co-Authors: Eliza Depoorter, Tom Coenye, Peter Vandamme, Charlotte Peeters, Matt J. Bull, Eshwar MahenthiralingamAbstract:Burkholderia is an incredibly diverse and versatile Gram-negative genus, within which over 80 species have been formally named and multiple other genotypic groups likely represent new species. Phylogenetic analysis based on the 16S rRNA gene sequence and core genome ribosomal multilocus sequence typing analysis indicates the presence of at least three major clades within the genus. Biotechnologically, Burkholderia are well-known for their bioremediation and biopesticidal properties. Within this review, we explore the ability of Burkholderia to synthesise a wide range of antimicrobial compounds ranging from historically characterised antifungals to recently described antibacterial antibiotics with activity against multiresistant clinical pathogens. The production of multiple Burkholderia antibiotics is controlled by quorum sensing and examples of quorum sensing pathways found across the genus are discussed. The capacity for antibiotic biosynthesis and secondary metabolism encoded within Burkholderia genomes is also evaluated. Overall, Burkholderia demonstrate significant biotechnological potential as a source of novel antibiotics and bioactive secondary metabolites.
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Use of quorum sensing inhibitors to interfere with biofilm formation and development in Burkholderia multivorans and Burkholderia cenocepacia
Research in microbiology, 2008Co-Authors: Gilles Brackman, Ulrik Hillaert, Serge Van Calenbergh, Hans J. Nelis, Tom CoenyeAbstract:Burkholderia cepacia complex strains are opportunistic pathogens causing life-threatening infections in cystic fibrosis patients. B. cepacia complex strains are resistant to many antimicrobial agents and commonly produce biofilms in vitro and in vivo. This contributes to their virulence and makes Burkholderia infections difficult to treat. Recently, the quorum sensing (QS) system of Burkholderia spp. has been found to affect their biofilm-forming ability, making it an attractive target for antimicrobial therapy. However, detailed information about the anti-biofilm effect of these compounds is still lacking. In the present study, we evaluated the anti-biofilm effect of several known QS inhibitors. The effect on Burkholderia spp. biofilm formation was examined using crystal violet, resazurin and SYTO9 staining, confocal laser scanning microscopy as well as plating. When used at subinhibitory concentrations, several compounds interfered with biofilm formation by Burkholderia spp. Our results suggest that the QS inhibitors affect later stages of biofilm formation and detachment.
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Diversity and occurrence of Burkholderia spp. in the natural environment
FEMS microbiology reviews, 2008Co-Authors: Stéphane Compant, Tom Coenye, Jerzy Nowak, Christophe Clément, Essaid Ait BarkaAbstract:Both in natural and in managed ecosystems, bacteria are common inhabitants of the phytosphere and the internal tissues of plants. Probably the most diverse and environmentally adaptable plant-associated bacteria belong to the genus Burkholderia. This genus is well-known for its human, animal and plant pathogenic members, including the Burkholderia cepacia complex. However, it also contains species and strains that are beneficial to plants and can be potentially exploited in biotechnological processes. Here we present an overview of plant-associated Burkholderia spp. with special emphasis on beneficial plant–Burkholderia interactions. A discussion of the potential for utilization of stable plant–Burkholderia spp. associations in the development of low-input cropping systems is also provided.
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Burkholderia : molecular microbiology and genomics
2007Co-Authors: Tom Coenye, Peter VandammeAbstract:1. Diversity and Role of Burkholderia spp. 2. Epidemiology, Typing and Population Genetics of Burkholderia Species 3. Comparative Genomics of Burkholderia Species 4. Antibiotic Resistance of Burkholderia spp. 5. Polysaccharides of Burkholderia spp. 6. Interaction of Burkholderia Species With the Phagocytic System 7. Beneficial Interactions of Burkholderia spp with Plants 8. The Phytopathogenic Burkholderia 9. Biodegradation of Organic Anthropogenic Pollutants by Burkholderia Species 10. Molecular Mechanisms of Virulence of Burkholderia pseudomallei and Burkholderia mallei 11. Model Systems of Burkholderia cepacia Complex Infection 12. Social Behavior of Members of the Genus Burkholderia: Quorum Sensing and Biofilms 13. Intracellular Survival of Burkholderia cepacia Complex Isolates
John J. Lipuma - One of the best experts on this subject based on the ideXlab platform.
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activity of tobramycin against cystic fibrosis isolates of Burkholderia cepacia complex grown as biofilms
Antimicrobial Agents and Chemotherapy, 2016Co-Authors: Sarah Kennedy, Trevor Beaudoin, Emma Caraher, James E A Zlosnik, Elizabeth Tullis, David P. Speert, John J. Lipuma, Valerie WatersAbstract:Pulmonary infection with Burkholderia cepacia complex in cystic fibrosis (CF) patients is associated with more-rapid lung function decline and earlier death than in CF patients without this infection. In this study, we used confocal microscopy to visualize the effects of various concentrations of tobramycin, achievable with systemic and aerosolized drug administration, on mature B. cepacia complex biofilms, both in the presence and absence of CF sputum. After 24 h of growth, biofilm thickness was significantly reduced by exposure to 2,000 μg/ml of tobramycin for Burkholderia cepacia, Burkholderia multivorans, and Burkholderia vietnamiensis; 200 μg/ml of tobramycin was sufficient to reduce the thickness of Burkholderia dolosa biofilm. With a more mature 48-h biofilm, significant reductions in thickness were seen with tobramycin at concentrations of ≥100 μg/ml for all Burkholderia species. In addition, an increased ratio of dead to live cells was observed in comparison to control with tobramycin concentrations of ≥200 μg/ml for B. cepacia and B. dolosa (24 h) and ≥100 μg/ml for Burkholderia cenocepacia and B. dolosa (48 h). Although sputum significantly increased biofilm thickness, tobramycin concentrations of 1,000 μg/ml were still able to significantly reduce biofilm thickness of all B. cepacia complex species with the exception of B. vietnamiensis. In the presence of sputum, 1,000 μg/ml of tobramycin significantly increased the dead-to-live ratio only for B. multivorans compared to control. In summary, although killing is attenuated, high-dose tobramycin can effectively decrease the thickness of B. cepacia complex biofilms, even in the presence of sputum, suggesting a possible role as a suppressive therapy in CF.
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Burkholderia humi sp. nov., Burkholderia choica sp. nov., Burkholderia telluris sp. nov., Burkholderia terrestris sp. nov. and Burkholderia udeis sp. nov.: Burkholderia glathei-like bacteria from soil and rhizosphere soil.
International Journal of Systematic and Evolutionary Microbiology, 2013Co-Authors: Peter Vandamme, Evie De Brandt, Kurt Houf, Joana Falcão Salles, Jan Dirk Van Elsas, Theodore Spilker, John J. LipumaAbstract:Analysis of partial gyrB gene sequences revealed six taxa in a group of 17 Burkholderia glathei-like isolates which were further examined by (GTG)(5)-PCR fingerprinting, 16S rRNA gene sequence analysis, DNA-DNA hybridizations, determination of the DNA G+C content, whole-cell fatty acid analysis and an analysis of cell and colony morphology and more than 180 biochemical characteristics. The results demonstrated that one taxon consisting of three human clinical isolates represented Burkholderia zhejiangensis, a recently described methyl-parathion-degrading bacterium isolated from a wastewater-treatment system in China. The remaining taxa represented five novel species isolated from soil or rhizosphere soil samples, and could be distinguished by both genotypic and phenotypic characteristics. We therefore propose to formally classify these bacteria as Burkholderia humi sp. nov. (type strain, LMG 22934(T)=CCUG 63059(T)), Burkholderia choica sp. nov. (type strain, LMG 22940(T)=CCUG 63063(T)), Burkholderia telluris sp. nov. (type strain, LMG 22936(T)=CCUG 63060(T)), Burkholderia udeis sp. nov. (type strain, LMG 27134(T)=CCUG 63061(T)) and Burkholderia terrestris sp. nov. (type strain, LMG 22937(T)=CCUG 63062(T)).
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Burkholderia latens sp nov., Burkholderia diffusa sp nov., Burkholderia arboris sp nov., Burkholderia seminalis sp nov and Burkholderia metallica sp nov., novel species within the Burkholderia cepacia complex
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2008Co-Authors: Elke Vanlaere, Deborah A Henry, David P. Speert, Eshwar Mahenthiralingam, John J. Lipuma, Evie De Brandt, Adam Baldwin, Christopher G. Dowson, Peter VandammeAbstract:The taxonomic position of five recA gene clusters of Burkholderia cepacia complex (Bcc) isolates was determined using a polyphasic taxonomic approach. The levels of 16S rRNA and recA gene sequence similarity, multilocus sequence typing (MLST) data and the intermediate DNA–DNA binding values demonstrated that these five clusters represented five novel species within the Bcc. Biochemical identification of these species is difficult, as is the case for most Bcc species. However, identification of these novel species can be accomplished through recA gene sequence analysis, MLST and BOX-PCR profiling and by recA RFLP analysis. For diagnostic laboratories, recA gene sequence analysis offers the best combination of accuracy and simplicity. Based on these results, we propose five novel Bcc species, Burkholderia latens sp. nov. (type strain FIRENZE 3T =LMG 24064T =CCUG 54555T), Burkholderia diffusa sp. nov. (type strain AU1075T =LMG 24065T =CCUG 54558T), Burkholderia arboris sp. nov. (type strain ES0263AT =LMG 24066T =CCUG 54561T), Burkholderia seminalis sp. nov. (type strain AU0475T =LMG 24067T =CCUG 54564T) and Burkholderia metallica sp. nov. (type strain AU0553T =LMG 24068T =CCUG 54567T). In the present study, we also demonstrate that Burkholderia ubonensis should be considered a member of the Bcc.
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Impact of Burkholderia Infection on Lung Transplantation in Cystic Fibrosis
American journal of respiratory and critical care medicine, 2008Co-Authors: Susan Murray, Jeffery Charbeneau, Bruce C. Marshall, John J. LipumaAbstract:Rationale: Lung transplantation offers the only survival option for patients with cystic fibrosis (CF) with end-stage pulmonary disease. Infection with Burkholderia species is typically considered a contraindication to transplantation in CF. However, the risks posed by different Burkholderia species on transplantation outcomes are poorly defined.Objectives: To quantify the risks of infection with Burkholderia species on survival before and after lung transplantation in patients with CF.Methods: Multivariate Cox survival models assessed hazard ratios of infection with Burkholderia species in 1,026 lung transplant candidates and 528 lung transplant recipients. Lung allocation scores, incorporating Burkholderia infection status, were calculated for transplant candidates.Measurements and Main Results: Transplant candidates infected with different Burkholderia species did not have statistically different mortality rates. Among transplant recipients infected with B. cenocepacia, only those infected with nonepid...
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development of a reca gene based identification approach for the entire Burkholderia genus
Applied and Environmental Microbiology, 2005Co-Authors: George William Payne, Tom Coenye, Peter Vandamme, John J. Lipuma, Sara H Morgan, Andrew J Weightman, Thomas Hefin Jones, Eshwar MahenthiralingamAbstract:Burkholderia is an important bacterial genus containing species of ecological, biotechnological, and pathogenic interest. With their taxonomy undergoing constant revision and the phenotypic similarity of several species, correct identification of Burkholderia is difficult. A genetic scheme based on the recA gene has greatly enhanced the identification of Burkholderia cepacia complex species. However, the PCR developed for the latter approach was limited by its specificity for the complex. By alignment of existing and novel Burkholderia recA sequences, we designed new PCR primers and evaluated their specificity by testing a representative panel of Burkholderia strains. PCR followed by restriction fragment length polymorphism analysis of an 869-bp portion of the Burkholderia recA gene was not sufficiently discriminatory. Nucleotide sequencing followed by phylogenetic analysis of this recA fragment differentiated both putative and known Burkholderia species and all members of the B. cepacia complex. In addition, it enabled the design of a Burkholderia genus-specific recA PCR that produced a 385-bp amplicon, the sequence of which was also able to discriminate all species examined. Phylogenetic analysis of 188 novel recA genes enabled clarification of the taxonomic position of several important Burkholderia strains and revealed the presence of four novel B. cepacia complex recA lineages. Although the recA phylogeny could not be used as a means to differentiate B. cepacia complex strains recovered from clinical infection versus the natural environment, it did facilitate the identification of clonal strain types of B. cepacia, B. stabilis, and B. ambifaria capable of residing in both niches.
Eshwar Mahenthiralingam - One of the best experts on this subject based on the ideXlab platform.
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Burkholderia: an update on taxonomy and biotechnological potential as antibiotic producers
Applied Microbiology and Biotechnology, 2016Co-Authors: Eliza Depoorter, Tom Coenye, Peter Vandamme, Charlotte Peeters, Matt J. Bull, Eshwar MahenthiralingamAbstract:Burkholderia is an incredibly diverse and versatile Gram-negative genus, within which over 80 species have been formally named and multiple other genotypic groups likely represent new species. Phylogenetic analysis based on the 16S rRNA gene sequence and core genome ribosomal multilocus sequence typing analysis indicates the presence of at least three major clades within the genus. Biotechnologically, Burkholderia are well-known for their bioremediation and biopesticidal properties. Within this review, we explore the ability of Burkholderia to synthesise a wide range of antimicrobial compounds ranging from historically characterised antifungals to recently described antibacterial antibiotics with activity against multiresistant clinical pathogens. The production of multiple Burkholderia antibiotics is controlled by quorum sensing and examples of quorum sensing pathways found across the genus are discussed. The capacity for antibiotic biosynthesis and secondary metabolism encoded within Burkholderia genomes is also evaluated. Overall, Burkholderia demonstrate significant biotechnological potential as a source of novel antibiotics and bioactive secondary metabolites.
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Burkholderia latens sp nov., Burkholderia diffusa sp nov., Burkholderia arboris sp nov., Burkholderia seminalis sp nov and Burkholderia metallica sp nov., novel species within the Burkholderia cepacia complex
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2008Co-Authors: Elke Vanlaere, Deborah A Henry, David P. Speert, Eshwar Mahenthiralingam, John J. Lipuma, Evie De Brandt, Adam Baldwin, Christopher G. Dowson, Peter VandammeAbstract:The taxonomic position of five recA gene clusters of Burkholderia cepacia complex (Bcc) isolates was determined using a polyphasic taxonomic approach. The levels of 16S rRNA and recA gene sequence similarity, multilocus sequence typing (MLST) data and the intermediate DNA–DNA binding values demonstrated that these five clusters represented five novel species within the Bcc. Biochemical identification of these species is difficult, as is the case for most Bcc species. However, identification of these novel species can be accomplished through recA gene sequence analysis, MLST and BOX-PCR profiling and by recA RFLP analysis. For diagnostic laboratories, recA gene sequence analysis offers the best combination of accuracy and simplicity. Based on these results, we propose five novel Bcc species, Burkholderia latens sp. nov. (type strain FIRENZE 3T =LMG 24064T =CCUG 54555T), Burkholderia diffusa sp. nov. (type strain AU1075T =LMG 24065T =CCUG 54558T), Burkholderia arboris sp. nov. (type strain ES0263AT =LMG 24066T =CCUG 54561T), Burkholderia seminalis sp. nov. (type strain AU0475T =LMG 24067T =CCUG 54564T) and Burkholderia metallica sp. nov. (type strain AU0553T =LMG 24068T =CCUG 54567T). In the present study, we also demonstrate that Burkholderia ubonensis should be considered a member of the Bcc.
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development of a reca gene based identification approach for the entire Burkholderia genus
Applied and Environmental Microbiology, 2005Co-Authors: George William Payne, Tom Coenye, Peter Vandamme, John J. Lipuma, Sara H Morgan, Andrew J Weightman, Thomas Hefin Jones, Eshwar MahenthiralingamAbstract:Burkholderia is an important bacterial genus containing species of ecological, biotechnological, and pathogenic interest. With their taxonomy undergoing constant revision and the phenotypic similarity of several species, correct identification of Burkholderia is difficult. A genetic scheme based on the recA gene has greatly enhanced the identification of Burkholderia cepacia complex species. However, the PCR developed for the latter approach was limited by its specificity for the complex. By alignment of existing and novel Burkholderia recA sequences, we designed new PCR primers and evaluated their specificity by testing a representative panel of Burkholderia strains. PCR followed by restriction fragment length polymorphism analysis of an 869-bp portion of the Burkholderia recA gene was not sufficiently discriminatory. Nucleotide sequencing followed by phylogenetic analysis of this recA fragment differentiated both putative and known Burkholderia species and all members of the B. cepacia complex. In addition, it enabled the design of a Burkholderia genus-specific recA PCR that produced a 385-bp amplicon, the sequence of which was also able to discriminate all species examined. Phylogenetic analysis of 188 novel recA genes enabled clarification of the taxonomic position of several important Burkholderia strains and revealed the presence of four novel B. cepacia complex recA lineages. Although the recA phylogeny could not be used as a means to differentiate B. cepacia complex strains recovered from clinical infection versus the natural environment, it did facilitate the identification of clonal strain types of B. cepacia, B. stabilis, and B. ambifaria capable of residing in both niches.
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Updated version of the Burkholderia cepacia complex experimental strain panel.
Journal of Clinical Microbiology, 2003Co-Authors: Tom Coenye, John R. W. Govan, Peter Vandamme, John J. Lipuma, Eshwar MahenthiralingamAbstract:The Burkholderia cepacia complex consists of nine closely related species: Burkholderia cepacia genomovars I and VI, Burkholderia multivorans (genomovar II), Burkholderia cenocepacia (genomovar III), Burkholderia stabilis (genomovar IV), Burkholderia vietnamiensis (genomovar V), Burkholderia
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diagnostically and experimentally useful panel of strains from the Burkholderia cepacia complex
Journal of Clinical Microbiology, 2000Co-Authors: Eshwar Mahenthiralingam, Tom Coenye, John R. W. Govan, David P. Speert, Jacqueline W Chung, Peter Taylor, Peter VandammeAbstract:Two new species, Burkholderia multivorans and Burkholderia vietnamiensis, and three genomovars (genomovars I, III, and IV) currently constitute the Burkholderia cepacia complex. A panel of 30 well-characterized strains representative of each genomovar and new species was assembled to assist with identification, epidemiological analysis, and virulence studies on this important group of opportunistic pathogens.
Monique Gillis - One of the best experts on this subject based on the ideXlab platform.
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Burkholderia fungorum sp nov and Burkholderia caledonica sp nov two new species isolated from the environment animals and human clinical samples
International Journal of Systematic and Evolutionary Microbiology, 2001Co-Authors: Tom Coenye, Monique Gillis, John R. W. Govan, Enevold Falsen, Anne Willems, Severine Laevens, Maria Ohlen, Wendy Hannant, Peter VandammeAbstract:A polyphasic taxonomic study that included DNA-DNA hybridizations, DNA base ratio determinations, 16S rDNA sequence analyses, whole-cell protein and fatty acid analyses and an extensive biochemical characterization was performed on 16 strains isolated from the environment, animals and human clinical samples. The isolates belonged to the genus Burkholderia, were phylogenetically closely related to Burkholderia graminis, Burkholderia caribensis and Burkholderia phenazinium and had G+C contents between 61.9 and 62.2 mol%. Seven strains isolated from the rhizosphere were assigned to Burkholderia caledonica sp. nov. [type strain LMG 19076T (= CCUG 42236T)]. Nine strains isolated from the environment, animals and human clinical samples were assigned to Burkholderia fungorum sp. nov. [type strain LMG 16225T (= CCUG 31961T)]. Differential tests for B. graminis, B. caribensis, B. phenazinium, B. caledonica and B. fungorum include the following: assimilation of trehalose, citrate, DL-norleucine, adipate and sucrose; nitrate reduction; growth in the presence of 0.5% NaCl; and beta-galactosidase activity.
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Burkholderia cepacia genomovar vi a new member of the Burkholderia cepacia complex isolated from cystic fibrosis patients
International Journal of Systematic and Evolutionary Microbiology, 2001Co-Authors: Tom Coenye, Deborah A Henry, Monique Gillis, David P. Speert, Katrien Vandemeulebroecke, Bart Hoste, John J. Lipuma, Peter VandammeAbstract:A polyphasic taxonomic study was performed on 23 strains isolated from cystic fibrosis (CF) patients in the USA. These strains were tentatively identified as Burkholderia cepacia, Burkholderia vietnamiensis and Burkholderia or Ralstonia sp. using biochemical tests and 16S rDNA-based PCR assays. Visual comparison of protein profiles indicated that they belonged to a single new group ('group 13'). The polyphasic taxonomic data showed that 18 of these strains represent a new member of the B. cepacia complex, referred to in this report as B. cepacia genomovar VI, whereas the other five strains belonged to Burkholderia multivorans. By means of biochemical tests, B. cepacia genomovar VI strains can be separated from B. cepacia genomovars I and III, Burkholderia stabilis, B. vietnamiensis and Burkholderia gladioli, but not from B. multivorans. Separation of B. cepacia genomovar VI and B. multivorans is possible using AFLP (amplified fragment length polymorphism) fingerprinting and DNA-DNA hybridizations. Retrospective analysis of epidemiological and genotypic data suggests that strains of B. cepacia genomovar VI have been involved in chronic colonization of CF patients and have been spread from person to person.
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Pseudomonas antimicrobica Attafuah and Bradbury 1990 is a junior synonym of Burkholderia gladioli (Severini 1913) Yabuuchi et al. 1993.
International Journal of Systematic and Evolutionary Microbiology, 2000Co-Authors: Tom Coenye, Monique Gillis, Peter VandammeAbstract:Comparison of the 16S rDNA sequence of Pseudomonas antimicrobica LMG 18920T with published 16S rDNA sequences from other pseudomonads indicated that Pseudomonas antimicrobica belongs to the genus Burkholderia, with Burkholderia gladioli, Burkholderia glumae and Burkholderia plantarii as its closest neighbours. DNA-DNA hybridizations confirmed that Pseudomonas antimicrobica and Burkholderia gladioli represent the same species. Strain LMG 18920T and other Burkholderia gladioli strains were also indistinguishable by SDS-PAGE of whole-cell proteins and had similar biochemical characteristics. The whole-cell fatty acid composition, however, was different from that of other Burkholderia gladioli strains. It is concluded that Pseudomonas antimicrobica is a later synonym of Burkholderia gladioli. As Burkholderia gladioli is known to cause infections in patients with cystic fibrosis and chronic granulomatous disease, the eventual use of strain LMG 18920T as a biological control agent should be approached with caution.
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Occurrence of multiple genomovars of Burkholderia cepacia in cystic fibrosis patients and proposal of Burkholderia multivorans sp. nov.
International Journal of Systematic and Evolutionary Microbiology, 1997Co-Authors: Peter Vandamme, H Revets, Sabine Lauwers, Renata Coopman, Tom Coenye, Monique Gillis, Barry Holmes, Bart Hoste, Marc Vancanneyt, Karel KerstersAbstract:We performed an integrated genotypic and phenotypic analysis of 128 strains of the genera Burkholderia, Ralstonia, and Pseudomonas in order to study the taxonomic structure of Burkholderia cepacia and its relationships with other Burkholderia species. Our data show that presumed B. cepacia strains isolated from cystic fibrosis patients belong to at least five distinct genomic species, one of which was identified as Burkholderia vietnamiensis. This group of five phenotypically similar species is referred to as the B. cepacia complex. The name Burkholderia multivorans is proposed for one of these genomic species, which was formerly referred to as B. cepacia genomovar II; the remaining B. cepacia groups are referred to as genomovars I, III, and IV, pending additional differential phenotypic tests. The role and pathogenic potential of each of these taxa, particularly in view of the potentially fatal infections in cystic fibrosis patients, need further evaluation. The data presented also demonstrate that Pseudomonas glathei and Pseudomonas pyrrocinia should be reclassified as Burkholderia species.
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Polyphasic Taxonomy in the Genus Burkholderia Leading to an Emended Description of the Genus and Proposition of Burkholderia vietnamiensis sp. nov. for N2-Fixing Isolates from Rice in Vietnam
International Journal of Systematic Bacteriology, 1995Co-Authors: Monique Gillis, Karel Kersters, Tran Van Van, René Bardin, Mart Goor, Prakash Hebbar, Anne Willems, P. Segers, Thierry Heulin, Maria P. FernandezAbstract:The taxonomic position of nitrogen-fixing strains that were isolated from rhizosphere macerates of rice cultivated in the Binh Thanh region of Vietnam was determined by using polyphasic taxonomy. We determined the phylogenetic relationships of these organisms by performing DNA-rRNA hybridization experiments with a labeled rRNA probe from the type strain of Burkholderia cepacia, and we found that they belong to a single rRNA complex. Other members of this rRNA complex were also studied, and the N2-fixing strains were found to be closely related to B. cepacia. In addition, all members of the rRNA complex containing B. cepacia were studied by performing auxanographic and DNA-DNA hybridization experiments. Phenotypically and genotypically, the N2-fixing isolates constitute a single cluster together with two strains of clinical origin. These organisms constitute a new Burkholderia species, for which the name Burkholderia vietnamiensis is proposed; the type strain of this species is TVV75 (= LMG 10929). All members of this species can fix nitrogen. On the basis of our polyphasic taxonomy results and previously published data we concluded that the genus Burkholderia should be restricted to the following species: B. cepacia (the type species), Burkholderia mallei, Burkholderia pseudomallei, B. vietnamiensis, Burkholderia gladioli, Burkholderia caryophylli, Burkholderia plantarii, Burkholderia glumae, Burkholderia vandii, Burkholderia cocovenenans comb. nov., and Burkholderia andropogonis comb. nov. On the basis of genotypic and phenotypic results [Alcaligenes] eutrophus, [Burkholderia] solanacearum, and [Burkholderia] pickettii belong to two other clusters whose internal structures must be studied further.
