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Osamu Tanaka - One of the best experts on this subject based on the ideXlab platform.

  • Histocytochemical and immunohistochemical studies related to the role of glycogen in human developing digestive organs
    Anatomy and Embryology, 1995
    Co-Authors: Koji Hashimoto, Hiroki Otani, Katsuhiro Tamura, Osamu Tanaka
    Abstract:

    To elucidate the role of glycogen in the epithelium of developing digestive organs, we investigated the appearance of glycogen and glycogen phosphorylase (GP) in these organs. We studied 64 externally normal human embryos at Carnegie Stages 13–23 (5.1–28.0 mm in crown-ramp length, 4–8 weeks of gestation) by histocytochemical staining for glycogen and immunohistochemical staining with antibodies against two isoenzymes of GP: brain-type (BGP) and mucle-brain-type (MBGP) GP. At stage 13, glycogen appeared in the epithelium of the digestive tract and the parenchyma of the pancreas. As development advanced, glycogen granules increased in number and size in these tissues, and they became evenly distributed in the epithelium of the digestive tract as either single particles or aggregates, as deduced by electron microscopy at late embryonic Stages. Immunoreactivity specific both for BGP and for MBGP was detected in the digestive tract and the pancreas from stage 13. As development advanced, both BGP- and MBGP-immunoreactive cells increased in number and in immunoreactivity, and the number of MBGP-immunoreactive cells became larger than that of BGP-immunoreactive cells. By contrast, in hepatic cells, which serve as a major storage site for glycogen in adults, glycogen was detected only from stage 20, in smaller amounts, without formation of aggregates, and no immunoreactivity specific for BGP or MBGP was apparent throughout the embryonic Stages examined. Thus, in the epithelium of the digestive tract and the parenchyma of the pancreas, but not in hepatic cells, the appearance and localization of GP coincided almost exactly with that of glycogen. These observations suggest that glycogen in the epithelium of the digestive tract and the parenchyma of the pancreas has not only been synthesized but also degraded from an early embryonic period and may, thus, be related to active cellular metabolism that is specific for embryonic development, including proliferation of the epithelium and interactions between epithelium and mesenchyme.

  • Ultrastructure of the developing stomach in human embryos
    Anatomy and Embryology, 1993
    Co-Authors: Hiroki Otani, Ryuju Hashimoto, Toshihisa Hatta, Tsunao Yoneyama, Osamu Tanaka
    Abstract:

    Ultrastructural development of the stomach was studied by light, scanning electron and transmission electron microscopy, using 19 human embryos at Carnegie Stages from 14 to 23 (6.8–28.0 mm in crown-rump length, 5 to 8 weeks of gestation). The precise time of appearance of differentiated characteristic structures was examined electron microscopically. The first gastric pit, with radially arranged epithelial cells beneath which the basement membrane bulged into the mesenchyme, was observed on the lesser curvature at stage 22. Although the mesenchymal condensation which would develop into the inner circular muscle layer appeared at stage 18 onward, cytoplasmic myofibrils were not observed until stage 22. Nerve fibers were first observed at stage 16, and at later Stages they gathered into bundles to form a nerve plexus external to the developing inner circular muscle layer. On the basis of accurate timing of the appearance and the mode of development of these structures, possible relations between developing gastric layers were discussed. Histocytochemically, glycogen or other carbohydrates were demonstrated in the cytoplasm of the gastric epithelium throughout the Stages examined. These carbohydrates were localized mainly in vacuole-like spaces in the basal part of the epithelial cells. This subcellular localization, and the amount of carbohydrate, did not change significantly during the observed embryonic period. In the serosa, carbohydrates were not detected at Stages 14 and 15, but observed consistently within the vacuoles in the cytoplasm from stage 17 onward. No other layer of the embryonic stomach had detectable carbohydrates. These observations suggest that carbohydrates in the gastric epithelium at an early developmental stage are not directly related to the developing mucin secretory activity of the epithelium, but may serve as an energy source for cell growth and differentiation of the epithelium and/or for mesenchyme-epithelial interactions.

  • Stage-specific expression of cancer-associated type 1 and type 2 chain polylactosamine antigens in the developing pancreas of human embryos.
    Cancer research, 1992
    Co-Authors: Xiao-hui Tuo, Osamu Tanaka, Shigeyuki Itai, Junko Nishikata, Tohru Mori, Reiji Kannagi
    Abstract:

    Expression of type 1 and type 2 chain carbohydrate antigens during the course of morphogenesis of human embryonic pancreas was investigated using specific monoclonal antibodies and compared with the carbohydrate antigen profiles of human pancreatic cancers. The type 2 chain antigens, such as stage-specific embryonic antigen 1 (Lex) and I-antigens, appeared much earlier than the type 1 chain antigens; the epithelial cells of primitive foregut were Lex+I-antigen- in the embryos at Carnegie Stages 16–23, while the pancreatic primordial cells, which had differentiated from the Lex+ gut epithelial cells, were Lex--I-antigen+ at Carnegie Stages 22–23. The type 1 chain antigens, such as Lea, Leb, Lec, and their sialylated derivatives, were not expressed in any cells at these Stages and appeared much later in the pancreas of the 10–12-week embryos, when the primitive pancreatic ductal cells in the primordia exhibited an extensive budding of the daughter cells. At this stage, Lea appeared and was expressed strongly in the epithelial cells of primitive pancreatic ducts as well as in the daughter cells that were destined to differentiate into future centroacinar cells; Leb was localized in the daughter cells which were to become future acinar cells; and Lec was specifically expressed in the daughter cells which were to form future Langerhans islets. With regard to the sialylated derivatives of Lea, expression of the 2–3 sialyl Lea antigen was limited to the epithelial cells of the primitive pancreatic ducts, while the 2–6 sialyl Lea antigen was strongly expressed in the future centroacinar cells, which had differentiated from the corresponding daughter cells. Among these antigens, the Lea and 2–3 sialyl Lea antigens showed the highest incidence in human pancreatic cancer tissues. These results indicate that the expression of these carbohydrate antigens in embryonic pancreas is differentiation dependent and cell lineage specific and that most human pancreatic cancer cells mimic the carbohydrate antigen profile of the epithelial cells of the primitive pancreatic ducts in human embryos.

  • Timing and sequence of the events in the development of extraocular muscles in staged human embryos: ultrastructural and histochemical study.
    Cells Tissues Organs, 1992
    Co-Authors: M. Oguni, T. Setogawa, H. Matsui, Ryuju Hashimoto, Osamu Tanaka
    Abstract:

    The ultrastructure and the appearance of glycogen were studied in the extraocular muscles of 14 externally normal human embryos (Carnegie Stages 13–21). At stage 16, myofibrils with an immature Z line

  • Immunohistochemical Study of Carbonic Anhydrase III in the Extraocular Muscles of Human Embryos
    Cells Tissues Organs, 1992
    Co-Authors: M. Oguni, T. Setogawa, Osamu Tanaka, Haruo Shinohara, K. Kato
    Abstract:

    The differentiation of extraocular muscles was studied immunohistochemically in externally normal human embryos (Carnegie Stages 13-23), using antibodies to carbonic anhydrase (CA) III and β-enolase as the markers of type 1 and type 2 muscle fibers, respectively. At stage 18, some myoblasts were immunoreactive to β-enolase antibodies, however, CA-III immunoreactivity was not observed around the optic vesicle. At stage 20, CA-III immunoreactivity appeared in some muscle fibers of extraocular muscles. From stage 21 to stage 23, CA-III-immunoreactive fibers increased and almost equalled the number of β-enolase-immunoreactive fibers. These findings suggest that CA-III-immunoreactive type 1 fibers appear in the late stage of myogenesis compared with β-enolase-immunoreactive type 2 fibers.

G. Godlewski - One of the best experts on this subject based on the ideXlab platform.

  • Moving and fusion of the pancreatic buds in the rat embryos during the embryonic period (Carnegie Stages 13-17) by a three-dimensional computer-assisted reconstruction.
    Surgical and Radiologic Anatomy, 2011
    Co-Authors: G. Godlewski, M. Radi, J. Gaubert, R. Cristol-gaubert, Volker Baecker, Pierre Travo, M. Prudhomme, D. Prat-pradal
    Abstract:

    Aim The purpose of the present study was to illustrate the modality of rotation of ventral and dorsal pancreatic buds by three-dimensional (3D) reconstructions in the rat embryos, during the Carnegie Stages 13–17.

  • A 3D reconstruction of pancreas development in the human embryos during embryonic period (Carnegie Stages 15–23)
    Surgical and Radiologic Anatomy, 2010
    Co-Authors: M. Radi, G. Godlewski, J. Gaubert, R. Cristol-gaubert, Volker Baecker, Pierre Travo, M. Prudhomme, D. Prat-pradal
    Abstract:

    Aims The goal in this paper was to rebuild a three dimensional (3D) reconstruction of the dorsal and ventral pancreatic buds, in the human embryos, at Carnegie Stages 15–23. Method The early development of the pancreas is studied by tissue observation and reconstruction by a computer-assisted method, using a light micrograph images from consecutive serial sagittal sections (diameter 7 μm) of ten human embryos ranging from Carnegie Stages 15–23, CRL 7–27 mm, fixed, dehydrated and embedded in paraffin, were stained alternately with haematoxylin–eosin or Heindenhain’Azan. The images were digitalized by Canon Camera 350 EOS D. The serial views were aligned automatically by software, manual alignment was performed, the data were analysed following segmentation and threshold. Results The two buds were clearly identified at stage 15. In stage 16, both pancreatic buds were in final position, and begin to merge in stage 17. From stage 18 to the stage 23, surrounding connective tissue differentiated. In the stage 23, the morphology of the pancreas was definitive. The superior portion of the anterior face of the pancreas’s head was arising from the dorsal bud. The rest of the head including the uncinate process emanated from the ventral bud. Conclusion The 3D computer-assisted reconstruction of the human pancreas visualized the relationships between the two pancreatic buds. This explains the disposition and the modality of the components fusion. This embryologic development permits a better understanding of congenital abnormalities.

  • A 3D reconstruction of pancreas development in the human embryos during embryonic period (Carnegie Stages 15-23).
    Surgical and Radiologic Anatomy, 2009
    Co-Authors: M. Radi, G. Godlewski, J. Gaubert, R. Cristol-gaubert, Volker Baecker, Pierre Travo, M. Prudhomme, D. Prat-pradal
    Abstract:

    Aims The goal in this paper was to rebuild a three dimensional (3D) reconstruction of the dorsal and ventral pancreatic buds, in the human embryos, at Carnegie Stages 15–23.

  • Three-dimensional computer-assisted reconstruction of ductal plate in the rat embryo (Carnegie Stages 19–23)
    Surgical and Radiologic Anatomy, 2004
    Co-Authors: G. Godlewski, R. Gaubert-cristol, J. Gaubert, M. Dauzat, F. Aldréa, M. Prudhomme
    Abstract:

    In bile duct morphogenesis it has been established that the extrahepatic bile ducts in human originate from hepatic diverticulum while intrahepatic bile ducts arise from the ductal plate (DP), a network of primitive biliary epithelium that develops in the periportal connective tissue. The aim of this work was to reconstruct in rat embryos, Stages 19–23, the three-dimensional (3D) distribution of the DP by means of a computer-assisted method. Six specimens, Stages 19–23, fixed, dehydrated and paraffin-embedded, were submitted to serial histological sections and stained by hematoxylin-eosin and Heidenhain techniques. The images were directly digitalized with a CCD camera. The serial views were aligned anatomically by software and the data were analyzed following segmentation and thresholding. At stage 19, the DP was not yet organized. The periportal mesoderm (M) was gaining ground with some cords of cubic cells evoking primitive ductal cells. At stage 20, a row of ductal cubic cells went around the transverse portal sinus at the junction between M and liver cells. At stage 21, the DP developed at the periphery of periportal connective tissue and appeared in direct continuity with the hepatic duct (HDu). Four evaginations emerged from the DP and were growing up in the hepatic parenchyma. At stage 23, the DP appeared as a large network in continuity with the HDu located at the periphery of periportal M and presenting several evaginations radiating in the liver parenchyma. This work in the rat embryo permits the clear visualization of the development of the junctional zone in the hepatic hilum. Three phenomena are observed: (1) proximal left and right hepatic ducts and their segmental branches are derived from DP and not from the HDu; (2) the extrahepatic biliary system is in contact with the developing hilar ducts; (3) ductal maturation begins at the hilum and proceeds centrifugally. These observations are of great relevance in explaining pathological changes appearing at the hepatic hilum of neonates: hepatic polycystic disease, intrahepatic bile duct agenesis or atresia, and cyst of the extrahepatic bile duct.

  • three dimensional computer assisted reconstruction of ductal plate in the rat embryo Carnegie Stages 19 23
    Surgical and Radiologic Anatomy, 2004
    Co-Authors: G. Godlewski, R Gaubertcristol, J. Gaubert, M. Dauzat, F. Aldréa, M. Prudhomme
    Abstract:

    In bile duct morphogenesis it has been established that the extrahepatic bile ducts in human originate from hepatic diverticulum while intrahepatic bile ducts arise from the ductal plate (DP), a network of primitive biliary epithelium that develops in the periportal connective tissue. The aim of this work was to reconstruct in rat embryos, Stages 19–23, the three-dimensional (3D) distribution of the DP by means of a computer-assisted method. Six specimens, Stages 19–23, fixed, dehydrated and paraffin-embedded, were submitted to serial histological sections and stained by hematoxylin-eosin and Heidenhain techniques. The images were directly digitalized with a CCD camera. The serial views were aligned anatomically by software and the data were analyzed following segmentation and thresholding. At stage 19, the DP was not yet organized. The periportal mesoderm (M) was gaining ground with some cords of cubic cells evoking primitive ductal cells. At stage 20, a row of ductal cubic cells went around the transverse portal sinus at the junction between M and liver cells. At stage 21, the DP developed at the periphery of periportal connective tissue and appeared in direct continuity with the hepatic duct (HDu). Four evaginations emerged from the DP and were growing up in the hepatic parenchyma. At stage 23, the DP appeared as a large network in continuity with the HDu located at the periphery of periportal M and presenting several evaginations radiating in the liver parenchyma. This work in the rat embryo permits the clear visualization of the development of the junctional zone in the hepatic hilum. Three phenomena are observed: (1) proximal left and right hepatic ducts and their segmental branches are derived from DP and not from the HDu; (2) the extrahepatic biliary system is in contact with the developing hilar ducts; (3) ductal maturation begins at the hilum and proceeds centrifugally. These observations are of great relevance in explaining pathological changes appearing at the hepatic hilum of neonates: hepatic polycystic disease, intrahepatic bile duct agenesis or atresia, and cyst of the extrahepatic bile duct.

M. Prudhomme - One of the best experts on this subject based on the ideXlab platform.

  • Three-dimensional computer-assisted reconstruction of ductal plate in the rat embryo (Carnegie Stages 19–23)
    Surgical and Radiologic Anatomy, 2004
    Co-Authors: G. Godlewski, R. Gaubert-cristol, J. Gaubert, M. Dauzat, F. Aldréa, M. Prudhomme
    Abstract:

    In bile duct morphogenesis it has been established that the extrahepatic bile ducts in human originate from hepatic diverticulum while intrahepatic bile ducts arise from the ductal plate (DP), a network of primitive biliary epithelium that develops in the periportal connective tissue. The aim of this work was to reconstruct in rat embryos, Stages 19–23, the three-dimensional (3D) distribution of the DP by means of a computer-assisted method. Six specimens, Stages 19–23, fixed, dehydrated and paraffin-embedded, were submitted to serial histological sections and stained by hematoxylin-eosin and Heidenhain techniques. The images were directly digitalized with a CCD camera. The serial views were aligned anatomically by software and the data were analyzed following segmentation and thresholding. At stage 19, the DP was not yet organized. The periportal mesoderm (M) was gaining ground with some cords of cubic cells evoking primitive ductal cells. At stage 20, a row of ductal cubic cells went around the transverse portal sinus at the junction between M and liver cells. At stage 21, the DP developed at the periphery of periportal connective tissue and appeared in direct continuity with the hepatic duct (HDu). Four evaginations emerged from the DP and were growing up in the hepatic parenchyma. At stage 23, the DP appeared as a large network in continuity with the HDu located at the periphery of periportal M and presenting several evaginations radiating in the liver parenchyma. This work in the rat embryo permits the clear visualization of the development of the junctional zone in the hepatic hilum. Three phenomena are observed: (1) proximal left and right hepatic ducts and their segmental branches are derived from DP and not from the HDu; (2) the extrahepatic biliary system is in contact with the developing hilar ducts; (3) ductal maturation begins at the hilum and proceeds centrifugally. These observations are of great relevance in explaining pathological changes appearing at the hepatic hilum of neonates: hepatic polycystic disease, intrahepatic bile duct agenesis or atresia, and cyst of the extrahepatic bile duct.

  • three dimensional computer assisted reconstruction of ductal plate in the rat embryo Carnegie Stages 19 23
    Surgical and Radiologic Anatomy, 2004
    Co-Authors: G. Godlewski, R Gaubertcristol, J. Gaubert, M. Dauzat, F. Aldréa, M. Prudhomme
    Abstract:

    In bile duct morphogenesis it has been established that the extrahepatic bile ducts in human originate from hepatic diverticulum while intrahepatic bile ducts arise from the ductal plate (DP), a network of primitive biliary epithelium that develops in the periportal connective tissue. The aim of this work was to reconstruct in rat embryos, Stages 19–23, the three-dimensional (3D) distribution of the DP by means of a computer-assisted method. Six specimens, Stages 19–23, fixed, dehydrated and paraffin-embedded, were submitted to serial histological sections and stained by hematoxylin-eosin and Heidenhain techniques. The images were directly digitalized with a CCD camera. The serial views were aligned anatomically by software and the data were analyzed following segmentation and thresholding. At stage 19, the DP was not yet organized. The periportal mesoderm (M) was gaining ground with some cords of cubic cells evoking primitive ductal cells. At stage 20, a row of ductal cubic cells went around the transverse portal sinus at the junction between M and liver cells. At stage 21, the DP developed at the periphery of periportal connective tissue and appeared in direct continuity with the hepatic duct (HDu). Four evaginations emerged from the DP and were growing up in the hepatic parenchyma. At stage 23, the DP appeared as a large network in continuity with the HDu located at the periphery of periportal M and presenting several evaginations radiating in the liver parenchyma. This work in the rat embryo permits the clear visualization of the development of the junctional zone in the hepatic hilum. Three phenomena are observed: (1) proximal left and right hepatic ducts and their segmental branches are derived from DP and not from the HDu; (2) the extrahepatic biliary system is in contact with the developing hilar ducts; (3) ductal maturation begins at the hilum and proceeds centrifugally. These observations are of great relevance in explaining pathological changes appearing at the hepatic hilum of neonates: hepatic polycystic disease, intrahepatic bile duct agenesis or atresia, and cyst of the extrahepatic bile duct.

  • Liver development in the rat and in man during the embryonic period (Carnegie Stages 11-23).
    Microscopy Research and Technique, 1997
    Co-Authors: G. Godlewski, R. Gaubert-cristol, S. Rouy, M. Prudhomme
    Abstract:

    Hepatic structures appearing during embryonic Carnegie Stages 11–23 were analyzed and compared in OFA-IOPS rat and human embryos. The group of rats—crown–rump length (CRL) 2–16 mm, 10–16 days postcoitus—was composed of 127 specimens (52 of Stages 11–12, 55 of Stages 13–19, and 20 of Stages 20–23), the human group of 9 embryos at Stages 14–23—CRL 5–31 mm, age 32–57 days—and human Stages 11–13 were described according to former literature. The specimens were subjected to serial histological sections with graphic reconstructions. In both series, stage 11 was characterized by hepatic diverticulum development, stage 12 and thereafter by cellular differentiation (septum transversum giving the liver stroma and hepatic diverticulum the hepatic trabeculae), and stage 13 by epithelial cord proliferation enmeshing stromal capillaries. From stage 14, the hepatic gland and its vascular channels presented considerable enlargement while hematopoietic function appeared. From this stage, the development of cystic primordium, never present in rat, was constant in man. At stage 18, after a period of obturation due to epithelial proliferation, the bile ducts became reorganized and ensured the continuity between liver cells and gut. From Stages 18 to 23, biliary ductules developed in periportal connective tissue producing ductal plates that received biliary capillaries. Except for gallbladder, similarity and presence of the same hepatic structures in man and rat during the embryonic period Stages 11–23 permit us to consider the rat as a good experimental model for liver development, for example, in studies on teratology and congenital anomalies. Microsc. Res. Tech. 39:314–327, 1997. © 1997 Wiley-Liss, Inc.

  • Liver Development in the Rat during the Embryonic Period (Carnegie Stages 15–23)
    Cells Tissues Organs, 1997
    Co-Authors: G. Godlewski, R. Gaubert-cristol, S. Rouy, M. Prudhomme
    Abstract:

    Hepatic and biliary structures appearing during embryonic Carnegie Stages 15–23 were analyzed in OFA rat embryos. The group of rats with crown rump length 7.5–16 mm and 12.8–16 days after coitus yielded 55 specimens (23 of Stages 15–16, 20 of Stages 17–18, 9 of Stages 19–22 and 3 of stage 23). The embryos were submitted to serial histological sections with graphic reconstructions. From stage 15 to the end of the embryonic period, the hepatic gland and its vascular channels (transverse portal sinus and hepatocardiac veins) presented considerable enlargement while hematopoietic function appeared. At stage 17, occlusion due to epithelial proliferation was evident in the hepatic duct. At stage 18, the duct became recanalized and assured the continuity between liver cells and gut. From stage 18 to 23, biliary ductules developed in periportal connective tissue forming ductal plates which received biliary caniculi. Except for the gallbladder which is never present in the rat, similarity and presence of the same hepatic structures in man and in the rat during the embryonic period Stages 15–23 suggest that the rat is a good experimental model for liver development and will be useful in the understanding of congenital anomalies.

  • liver development in the rat during the embryonic period Carnegie Stages 15 23
    Cells Tissues Organs, 1997
    Co-Authors: G. Godlewski, S. Rouy, R Gaubertcristol, M. Prudhomme
    Abstract:

    Hepatic and biliary structures appearing during embryonic Carnegie Stages 15–23 were analyzed in OFA rat embryos. The group of rats with crown rump length 7.5–16 mm and 12.8–16 days after coitus yielded 55 specimens (23 of Stages 15–16, 20 of Stages 17–18, 9 of Stages 19–22 and 3 of stage 23). The embryos were submitted to serial histological sections with graphic reconstructions. From stage 15 to the end of the embryonic period, the hepatic gland and its vascular channels (transverse portal sinus and hepatocardiac veins) presented considerable enlargement while hematopoietic function appeared. At stage 17, occlusion due to epithelial proliferation was evident in the hepatic duct. At stage 18, the duct became recanalized and assured the continuity between liver cells and gut. From stage 18 to 23, biliary ductules developed in periportal connective tissue forming ductal plates which received biliary caniculi. Except for the gallbladder which is never present in the rat, similarity and presence of the same hepatic structures in man and in the rat during the embryonic period Stages 15–23 suggest that the rat is a good experimental model for liver development and will be useful in the understanding of congenital anomalies.

Eric A.p. Steegers - One of the best experts on this subject based on the ideXlab platform.

  • Effect of human embryonic morphological development on fetal growth parameters: the Rotterdam Periconceptional Cohort (Predict Study).
    Reproductive BioMedicine Online, 2019
    Co-Authors: F. Parisi, M. Rousian, Anton H. J. Koning, Sten P. Willemsen, Eric A.p. Steegers, Régine P.m. Steegers-theunissen
    Abstract:

    Abstract Research question Is embryonic morphological development according to the Carnegie Stages associated with pregnancy outcome? Design In a tertiary hospital-based cohort, 182 singleton non-malformed pregnancies were selected. Serial transvaginal three-dimensional ultrasound (3D-US) scans were carried out between 6+0 and 10+2 gestational weeks. Embryonic development was annotated according to the morphological criteria of the Carnegie classification using a virtual reality system. Second-trimester biparietal diameter, head circumference, abdominal circumference and femur length measurements were retrieved from medical records. Z-scores were calculated for mid-pregnancy estimated fetal weight (EFW) and newborn birth weight. Associations between longitudinal Carnegie Stages and fetal growth parameters were investigated using linear mixed models, with subgroup analysis based on fetal gender. Results A total of 576 first-trimester 3D-US scans were analysed (median of three scans per pregnancy). Embryonic development was positively associated with EFW z-score (β = 0.69; 95% CI 0.51 to 0.86; P Conclusions Human embryonic development according to the Carnegie Stages is associated with fetal growth parameters with gender-specificity of birth weight. These results emphasize the importance of the first-trimester of pregnancy, raising the morphological staging of the embryo as a new methodology for early risk assessment and improvement of subsequent fetal growth parameters.

  • Early first trimester maternal ‘high fish and olive oil and low meat’ dietary pattern is associated with accelerated human embryonic development
    European Journal of Clinical Nutrition, 2018
    Co-Authors: Francesca Parisi, Anton H. J. Koning, Sten P. Willemsen, Irene Cetin, Régine P.m. Steegers-theunissen, Melek Rousian, Jeanne H. M. Vries, Eric A.p. Steegers
    Abstract:

    Background/objectives Maternal dietary patterns were associated with embryonic growth and congenital anomalies. We aim to evaluate associations between early first trimester maternal dietary patterns and embryonic morphological development among pregnancies with non-malformed outcome. Subjects/methods A total of 228 strictly dated, singleton pregnancies without congenital malformations were enrolled in a periconceptional hospital-based cohort. Principal component analysis was performed to extract early first trimester maternal dietary patterns from food frequency questionnaires. Serial transvaginal three-dimensional ultrasound (3D US) scans were performed between 6^+0 and 10^+2 gestational weeks and internal and external morphological criteria were used to define Carnegie Stages in a virtual reality system. Associations between dietary patterns and Carnegie Stages were investigated using linear mixed models. Results A total of 726 3D US scans were included (median: three scans per pregnancy). The ‘high fish and olive oil and low meat’ dietary pattern was associated with accelerated embryonic development in the study population ( β  = 0.12 (95%CI: 0.00; 0.24), p  

  • Early first trimester maternal 'high fish and olive oil and low meat' dietary pattern is associated with accelerated human embryonic development.
    European Journal of Clinical Nutrition, 2018
    Co-Authors: F. Parisi, M. Rousian, Anton H. J. Koning, Sten P. Willemsen, Irene Cetin, Régine P.m. Steegers-theunissen, Jeanne H. M. Vries, Eric A.p. Steegers
    Abstract:

    Background/objectives: Maternal dietary patterns were associated with embryonic growth and congenital anomalies. We aim to evaluate associations between early first trimester maternal dietary patterns and embryonic morphological development among pregnancies with non-malformed outcome. Subjects/methods: A total of 228 strictly dated, singleton pregnancies without congenital malformations were enrolled in a periconceptional hospital-based cohort. Principal component analysis was performed to extract early first trimester maternal dietary patterns from food frequency questionnaires. Serial transvaginal three-dimensional ultrasound (3D US) scans were performed between 6+0 and 10+2 gestational weeks and internal and external morphological criteria were used to define Carnegie Stages in a virtual reality system. Associations between dietary patterns and Carnegie Stages were investigated using linear mixed models. Results: A total of 726 3D US scans were included (median: three scans per pregnancy). The ‘high fish and olive oil and low meat’ dietary pattern was associated with accelerated embryonic development in the study population (β = 0.12 (95%CI: 0.00; 0.24), p < 0.05). Weak adherence to this dietary pattern delayed embryonic development by 2.1 days (95%CI: 1.6; 2.6) compared to strong adherence. The ‘high vegetables, fruit and grain’ dietary pattern accelerated embryonic development in the strictly dated spontaneous pregnancy subgroup without adjustment for energy intake. Conclusions: Early first trimester maternal dietary patterns impacts human embryonic morphological development among pregnancies without congenital malformations. The clinical meaning of delayed embryonic development needs further investigation.

Dianne N.irving - One of the best experts on this subject based on the ideXlab platform.