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J H Giliomee - One of the best experts on this subject based on the ideXlab platform.
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the identity of the wax scale insect Ceroplastes myricae linnaeus sternorrhyncha coccoidea coccidae and a key to the wax scales recorded from south africa
African Entomology, 2009Co-Authors: Chris J Hodgson, D J Williams, J H GiliomeeAbstract:What is believed to be the wax scale, Ceroplastes myricae (Linnaeus), has been rediscovered in the region of the type locality in South Africa on the type host plant, Morella quercifolia (L.) Killick (Myricaceae). The adult female is redescribed and illustrated and a neotype designated. In addition, a key to all species of wax scale insect known from South Africa is included.
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population dynamics of the white wax scale Ceroplastes destructor hemiptera coccidae on citrus in south africa with implications for biological control
Bulletin of Entomological Research, 2001Co-Authors: W M Wakgari, J H GiliomeeAbstract:The population dynamics of the white wax scale, Ceroplastes destructor Newstead, was studied intensively in four easy-peel citrus orchards in the Western Cape Province of South Africa over three consecutive years (1997-1999). Key factor analysis was used to determine and quantify the contribution of individual mortality factors to the total generation mortality. Key stage mortality, determined from a cohort life table, was in the third instar and pre-ovipositing female stages. Mortality of C. destructor was caused primarily by parasitoids, predators and miscellaneous factors. Parasitoids and miscellaneous factors acted as density-dependent regulatory agents during the pre-ovipositional and first instar stages respectively. This has implications for biological control of C. destructor. Some of the mortality factors acted either randomly with no reference to the population densities or in an inverse density-dependent manner during the egg-crawler, second or third instar stages.
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fecundity fertility and phenology of white wax scale Ceroplastes destructor newstead hemiptera coccidae on citrus and syzygium in south africa
African Entomology, 2000Co-Authors: W M Wakgari, J H GiliomeeAbstract:The population density of Ceroplastes destructor Newstead has increased steadily since 1994, particularly on Citrus reticulata (Blanco), in'citrus-growing areas of the Western and Eastern Cape Provinces of Sou th Africa. The fecundity, body size and phenology of C. destructor were studied to provide a more informed basis for control programmes. No significant differences in fecundity were found between orchards (P > 0.05). However, fecundity varied signifi cantly between female size-classes from the same orchard (P 0.05). Ceroplustes destructor had one generation per year in the Western Cape Province, South Africa. Oviposition commences in mid November and con tinues until the end of December, with a few females ovipositing until mid January. The decline in population density of the second ins tar in February was followed by a steady increase in that of the third instar. The third instar stage extended to the end of July followed by a peak population of adults in AUgust.
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description of the stages of the white wax scale Ceroplastes destructor newstead homoptera coccidae
African Entomology, 1998Co-Authors: W M Wakgari, J H GiliomeeAbstract:The white wax-scale, Ceroplastes destructor Newstead, has recently increased in numbers and distribution in some Easy-peel (Citrus reticulata (Blanco)) orchards in the Western Cape Province of South Africa. A study was conducted to investigate its morphology and biology. Characteristics of the immature and adult females are descriptionbed and illustrated from field-collected and slide-mounted specimens. A key to the different stages is provided. The biology of C. destructor is briefly descriptionbed. Morphometric characteristics useful for separating the stages are discussed.
Ana Lúcia Benfatti Gonzalez Peronti - One of the best experts on this subject based on the ideXlab platform.
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morphology of the immature female stages and the wax test of ten species of Ceroplastes hemiptera coccomorpha coccidae ceroplastinae from brazil
Zootaxa, 2016Co-Authors: Keila De Cassia C Rosa, Ana Lúcia Benfatti Gonzalez Peronti, Chris J Hodgson, Carlos Roberto SousasilvaAbstract:This paper describes the development of the waxy test and the microscopic characters of the three female nymphal instars of Ceroplastes cirripediformis Comstock, C. diospyros Hempel, C. floridensis Comstock, C. flosculoides Matile-Ferrero, C. formicarius Hempel, C. formosus Hempel, C. grandis Hempel, C. iheringi Cockerell, C . lucidus Hempel, and C. stellifer (Westwood). The discussion compares their morphology and divides the species into three groups according to the pattern of their dermal and wax characters.
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a revision of the wax scale insects hemiptera sternorrhyncha coccoidea ceroplastinae of the afrotropical region
Zootaxa, 2012Co-Authors: Chris J Hodgson, Ana Lúcia Benfatti Gonzalez PerontiAbstract:This paper reviews all species within the scale insect subfamily Ceroplastinae (Hemiptera: Sternorrhyncha: Coccoidea:Coccidae) known from the Afrotropical Region (here including the whole of Africa, the Atlantic islands of Azores,Madeira, Canary Is., Sao Tome, Principe, and Cape Verde Is., and the Indian Ocean islands of Madagascar, Seychelles,Mauritius, Reunion, Comoros, and Aldabra). At the start of the review, there were 64 species of wax scale insects andsubspecies in 4 genera recorded from this area. This paper describes or redescribes 58 species, 9 of them new, andsynonymises 18 species. We consider that these species are best placed in 19 species-groups and 2 genera, Ceroplastes Grey and Waxiella De Lotto, based on adult female morphology. Only 1 species and 1 subspecies previously recordedfrom Africa have not been seen (Waxiella erithraeus (Leonardi) and Waxiella mimosae neghelli (Bellio)). The preciseidentity of species close to C. rusci (Linnaeus) in Africa still requires further study, probably using molecular analysis asseveral records are considered to refer to cryptic or sibling species. The records of C. ceriferus (Fabricius) and C. actiniformis Green from Africa are considered to be misidentifications. The new species are: C. balachowskyi Hodgson& Peronti spec. nov., C. danieleae Hodgson & Peronti spec. nov., C. delottoi Hodgson & Peronti, spec. nov., C. elaeis Hodgson & Peronti, spec. nov., C. ghesquierei Hodgson & Peronti spec. nov., C. jos Hodgson & Peronti spec. nov., C. mori Hodgson & Peronti spec. nov., C. neobrachystegiae Hodgson & Peronti spec. nov. and C. newsteadi Hodgson P Ceroplastes candela Cockerell & King syn. nov. of Gascardia madagascariensis Targioni Tozzetti (now in Ceroplastes); C. combreti Brain syn. nov. of G. madagascariensis; C. uapacae Hall syn. nov. of C. personatus Newstead; C. vinsonioides Newstead syn. nov. of C. personatus; C. fumidus De Lotto syn. nov. of Coccus rusci Linnaeus (now in Ceroplastes); C. hololeucus De Lotto syn. nov. of C. singularis Newstead; C. spicatus Hall syn. nov. of C. eucleae Brain; C. coniformis Newstead syn. nov. of C. theobromae Newstead; C. constricta De Lotto syn. nov. of C. lamborni Newstead; Ceroplastes egbarum fulleri Cockerell & Cockerell syn. nov. of C. egbara Cockerell (now in Waxiella); Ceroplastes egbarum rhodesiensis Hall syn. nov. of C. egbara; Ceroplastes martinoi Almeida syn. nov. of C. egbara; Ceroplastes ugandae Newstead syn. nov. of C. egbara; Ceroplastes zonatus Newstead syn. nov. of C. egbara; Ceroplastes berliniae Hall syn. nov. of C. subsphaerica Newstead (now in Waxiella); Ceroplastes berlineae enkeldoorni Hall syn. nov. of C. subsphaerica; and Waxiella tamaricis Ben-Dov syn. nov. of C. mimosae Signoret (now in Waxiella). A neotype isdesignated for Coccus rusci Linnaeus (now Ceroplastes rusci (L.)). The subspecies Ceroplastes africanus senegalensis Marchal is here raised to specific rank and transferred to Waxiella as W. senegalensis (Marchal) stat. nov. Ceroplastes luteolus De Lotto, previously synonymised with C. brevicauda Hall, and C. tenuitectus Green, previously synonymisedwith C. rusci (L.), are here accepted as good species (stat. rev.). Ceroplastes cirripediformis Comstock is recorded fromAfrica (South Africa) for the first time. Waxiella uvariae (Marchal) is transferred back to Ceroplastes as C. uvariae Marchal stat. rev. Due to the large number of synonymies introduced here and in order to stabilise the status of some ofthe species, lectotypes have been designated for the following species: C. afrinanus senegalensis Marchal; C. berliniae Hall; C. berliniae enkeldoorni Hall; C. bipartitus Newstead; C. brevicauda Hall; C. coniformis Newstead; C. egbarum fulleri Cockerell & Cockerell; C. egbarum rhodesiensis Hall; C. elytropappi Brain; C. eucleae Brain; C. eugeniae Hall; C. ficus Newstead; C. galeatus Newstead; C. helichrysi Hall; C. lamborni Newstead; C. longicauda Brain; C. personatus Newstead; C. quadrilineatus Newstead; C. quadrilineatus simplex Brain; C. quadrilineatus royenae Hall; C. singularis Newstead; C. sinoiae Hall; C. tachardiaformis Brain; C. tenuitectus Green; C. toddaliae Hall; C. toddaliae spicatus Hall; C. uapacea Hall; C. uapacea chrysophyllae Hall; C. ugandae Newstead; C. uvariae Marchal; C. vinsonioides Newstead; C. vuilleti Marchal and C. zonatus Newstead. Keys are provided to identify the 19 species-groups recognised from thearea here studied and to all species in each species-group. There is a short final discussion, including such topics as thedistribution and the relationships of the species found on the Atlantic and Indian Ocean Islands. Maps are providedshowing the country distribution of each of the species covered and a list of plant species known as host of Ceroplastinae in the Afrotropical Region is appended.
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description of the immature female stages of Ceroplastes janeirensis gray hemiptera coccidae with a special reference to the development of the wax test
Zootaxa, 2011Co-Authors: Keila De Cassia C Rosa, Ana Lúcia Benfatti Gonzalez Peronti, Carlos Roberto SousasilvaAbstract:This paper describes the nymphal development of Ceroplastes janeirensis (Gray), which develops its wax test differently to that currently known for other Ceroplastinae. Macroscopic and microscopic characters of the three immature female stages are described and illustrated for the first time. An identification key to the female instars is provided and a brief discussion is also included comparing their morphology with those of other Ceroplastinae.
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revisao das especies de ceroplastinae atkinson hemiptera coccoidea coccidae do estado de sao paulo brasil
Revista Brasileira De Entomologia, 2008Co-Authors: Ana Lúcia Benfatti Gonzalez Peronti, Carlos Roberto Sousasilva, Maria Granara C De WillinkAbstract:The species of Ceroplastinae (Hemiptera, Coccoidea, Coccidae) from the state of Sao Paulo are revised. Thirty-six species are studied, 9 are synonymyzed, 8 new species are described and 19 known species are redescribed. Ceroplastes campinensis Hempel, 1901, C. bicolor Hempel, 1901, C. excaericae Hempel, 1912 and C. farmairii (Signoret, 1866) were not studied because the type material or other specimens were not found. Our studies indicate that C. albolineatus Cockerell, 1894 and C. confluens Cockerell & Tinsley, 1898 were erroneously cited by Hempel, 1900 as occurruing in the state of Sao Paulo. Coccus stellifer Westwood, 1871, currently Vinsonia stellifera (Westwood, 1871), is transferred to Ceroplastes as Ceroplastes stellifer (Westwood, 1871) n. comb. Ceroplastes flosculoides Matile-Ferrero, 1993 is recorded for the first time in Brazil and Ceroplastes cassiae (Chavannes, 1848), C. deodorensis Hempel, 1937, C. formosus Hempel, 1900 and C. quadratus Green, 1935 are recorded for the first time for state of Sao Paulo. The Ceroplastinae is now represented by 31 species in the state of Sao Paulo, all included in the genus Ceroplastes. Identification key and illustrations for species are included. New synonyms are proposed: C. formicarius Hempel = Ceroplastes communis Hempel, 1900 n. syn.; C. janeirensis Gray, 1828 = Ceroplastes psidii (Chavannes, 1848) n. syn. = C. simplex Hempel, 1900 n. syn.; C. cirripediformis Comstock, 1881 = C. cultus Hempel, 1900 n. syn. = C. cuneatus Hempel, 1900 n. syn. = C. rarus Hempel, 1900 n. syn and C. rotundus Hempel, 1900 n. syn.; C. lucidus Hempel, 1900 = C. novaesi Hempel, 1900 n. syn.; C. grandis Hempel, 1900 = C. rhizophorae Hempel, 1918 n. syn. New species described are: C. acutus sp. nov.; C. bragai sp. nov.; C. coronatus sp. nov; C. glomeratus sp. nov; C. jordanensis sp. nov.; C. minimus sp. nov.; C. solanaceus sp. nov.; C. willinkae sp. nov. Redescribed species are: C. agrestis Hempel, 1932; C. cassiae; C. cirripediformis; C. deodorensis; C. diospyros Hempel, 1928; C. floridensis Comstock, 1881; C. flosculoides; C. formicarius Hempel, 1900; C. formosus Hempel, 1900; C. grandis; C. gregarius Hempel, 1932; C. iheringi Cockerell, 1895; C. janeirensis; C. lucidus; C. purpureus Hempel, 1900; C. quadratus; C. speciosus Hempel, 1900; C. stellifer n. comb. and C. variegatus Hempel, 1900. Lectotypes and paralectotypes of C. agrestis, C. deodorensis, C. diospyros, C. purpureus, C. formosus, C. speciosus and C. variegatus and a neotype for C. cassiae are designated.
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Coccidae, Pseudococcidae, Ortheziidae, and Monophlebidae (Hemiptera: Coccoidea) of Espírito Santo, Brazil
Biota Neotropica, 2007Co-Authors: Mark P. Culik, Penny J Gullan, David Dos Santos Martins, José A. Ventura, Ana Lúcia Benfatti Gonzalez Peronti, Takumasa KondoAbstract:Culik, M.P., Martins, D.S., Ventura, J.A., Peronti, A.L.B.G., Gullan, P.Y. & Kondo, T. Coccidae, Pseudococcidae, Ortheziidae, and Monophlebidae (Hemiptera: Coccoidea) of Espirito Santo, Brazil. Biota Neotrop. Sep/Dez 2007 vol. 7, no. 3 http://www.biotaneotropica.org.br/v7n3/pt/abstract?article+bn00507032007. ISSN 1676-0603. New plant hosts are recorded for nine scale insect species recently collected in Espirito Santo, Brazil, and eleven scale insect species are recorded for the first time from the state: Ceroplastes floridensis Comstock, Coccus longulus (Douglas), Coccus viridis (Green), Eucalymnatus tesselatus (Signoret), Pseudokermes sp., Saissetia coffeae (Walker), Phenacoccus madeirensis Green, Pseudococcus jackbeardsleyi Gimpel & Miller, Pseudococcus longispinus (Targioni Tozzetti), Icerya purchasi Maskell, and Icerya genistae Hempel. This is also the first record of Co. longulus in Brazil. Information on the host plants and geographic distribution of the 26 species of scale insects of the families Coccidae, Pseudococcidae, Ortheziidae, and Monophlebidae, currently known from Espirito Santo is provided.
Penny J Gullan - One of the best experts on this subject based on the ideXlab platform.
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Coccidae, Pseudococcidae, Ortheziidae, and Monophlebidae (Hemiptera: Coccoidea) of Espírito Santo, Brazil
Biota Neotropica, 2007Co-Authors: Mark P. Culik, Penny J Gullan, David Dos Santos Martins, José A. Ventura, Ana Lúcia Benfatti Gonzalez Peronti, Takumasa KondoAbstract:Culik, M.P., Martins, D.S., Ventura, J.A., Peronti, A.L.B.G., Gullan, P.Y. & Kondo, T. Coccidae, Pseudococcidae, Ortheziidae, and Monophlebidae (Hemiptera: Coccoidea) of Espirito Santo, Brazil. Biota Neotrop. Sep/Dez 2007 vol. 7, no. 3 http://www.biotaneotropica.org.br/v7n3/pt/abstract?article+bn00507032007. ISSN 1676-0603. New plant hosts are recorded for nine scale insect species recently collected in Espirito Santo, Brazil, and eleven scale insect species are recorded for the first time from the state: Ceroplastes floridensis Comstock, Coccus longulus (Douglas), Coccus viridis (Green), Eucalymnatus tesselatus (Signoret), Pseudokermes sp., Saissetia coffeae (Walker), Phenacoccus madeirensis Green, Pseudococcus jackbeardsleyi Gimpel & Miller, Pseudococcus longispinus (Targioni Tozzetti), Icerya purchasi Maskell, and Icerya genistae Hempel. This is also the first record of Co. longulus in Brazil. Information on the host plants and geographic distribution of the 26 species of scale insects of the families Coccidae, Pseudococcidae, Ortheziidae, and Monophlebidae, currently known from Espirito Santo is provided.
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a cladistic analysis of wax scales hemiptera coccoidea coccidae ceroplastinae
Systematic Entomology, 1995Co-Authors: T K Qin, Penny J GullanAbstract:Seven genera of wax scales (Ceroplastinae) have been accepted as valid by some or all coecidologists: Ceroplastes Gray, Ceroplastidia Cockerell, Cerostegia De Lotto, Gascardia Targioni Tozzetti, Paracerostegia Tang, Vinsonia Signoret and Waxiella De Lotto. The status of most these genera is controversial. The present study reviews the taxonomic history of wax scales and presents a new classification based on cladistic analysis of morphological data from the adult females of eighty-three species. The analysis indicates that the wax scales form a monophyletic group, that Waxiella is monophyletic but that Ceroplastidia, Gascardia and Vinsonia are not, and fails to support the generic status of Cerostegia and Paracerostegia. Apart from the type species of Vinsonia, which was placed as a sister to the rest of wax scales, the only other species assigned to Vinsonia and most species of all other genera fall within Ceroplastes in the cladogram. Recognition of any of these genera renders Ceroplastes paraphyletic or polyphyletic. Thus, cladistically, the wax scales should be classified into one genus, Ceroplastes.
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The current distribution and geographical origin of the scale insect pest Ceroplastes sinensis (Hemiptera: Coccidae)
Bulletin of Entomological Research, 1994Co-Authors: Qin Ting-kui, G. Andrew C. Beattie, Peter S. Cranston, Penny J Gullan, John W. H. Trueman, Murray J Fletcher, D. P. A. SandsAbstract:Ceroplastes sinensis Del Guercio is a pest of commercial citrus in Australia and has been recorded from New Zealand, southern Europe, northern Africa, North America and Mexico. Its distribution is mainly temperate and it is more restricted climatically than previously believed. Uncertainty concerning the place of origin of C. sinensis has thwarted previous attempts at biological control. In the present study, a novel and cost-effective approach using cladistic estimates is demonstrated to determine the place of its origin. Cladistically derived phylogenies are commonplace for many organisms, but the method has not been used before to predict the area of origin of a cosmopolitan pest. Cladistic analysis of the wax scales predicts a native range for C. sinensis within Central or South America. This prediction appears to have been confirmed by the recent discovery of heavily parasitized individuals of C. sinensis in Argentina. The cladistic analysis also allows prediction of the geographical origin of three other widespread wax scale speries: Ceroplastes ceriferus (Fabricius) may be of Neotropical origin, C. rubens Maskell and C. rusci (Linnaeus) are probably native to the Afrotropical region. The approach used has wide implications because the native areas of many other economically important pests are still unknown or uncertain.
W M Wakgari - One of the best experts on this subject based on the ideXlab platform.
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population dynamics of the white wax scale Ceroplastes destructor hemiptera coccidae on citrus in south africa with implications for biological control
Bulletin of Entomological Research, 2001Co-Authors: W M Wakgari, J H GiliomeeAbstract:The population dynamics of the white wax scale, Ceroplastes destructor Newstead, was studied intensively in four easy-peel citrus orchards in the Western Cape Province of South Africa over three consecutive years (1997-1999). Key factor analysis was used to determine and quantify the contribution of individual mortality factors to the total generation mortality. Key stage mortality, determined from a cohort life table, was in the third instar and pre-ovipositing female stages. Mortality of C. destructor was caused primarily by parasitoids, predators and miscellaneous factors. Parasitoids and miscellaneous factors acted as density-dependent regulatory agents during the pre-ovipositional and first instar stages respectively. This has implications for biological control of C. destructor. Some of the mortality factors acted either randomly with no reference to the population densities or in an inverse density-dependent manner during the egg-crawler, second or third instar stages.
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the current status of the biocontrol of Ceroplastes destructor newstead hemiptera coccidae on citrus and syzygium in south africa
Biocontrol Science and Technology, 2001Co-Authors: W M WakgariAbstract:The incidence of parasitism and predation of the third instar and adult Ceroplastes destructor Newstead (Hemiptera: Coccidae) on Citrus reticulata (Blanco) and Syzygium (= Eugenia ) malaccensis (L.) in the Western Cape, South Africa, was assessed from June 1997 to December 1999. Seven primary and three secondary parasitoids, as well as four predator species were identified. Aprostocetus (= Tetrastichus ) ceroplastae (Girault) was the predominant parasitoid species, accounting for 78.9% of the total primary parasitoids. Peak numbers of parasitoids and predators were synchronized with peak emergence of the scale stages susceptible to parasitism and predation, indicating that the scale-parasitoid/predator association contained a density-dependent regulatory mechanism. Parasitoids demonstrated density-dependence at the third instar and pre-ovipositing female stage for scales on C. reticulata and S. malaccensis , respectively, whereas predators acted as a density-dependent mortality factor only during the pre-...
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fecundity fertility and phenology of white wax scale Ceroplastes destructor newstead hemiptera coccidae on citrus and syzygium in south africa
African Entomology, 2000Co-Authors: W M Wakgari, J H GiliomeeAbstract:The population density of Ceroplastes destructor Newstead has increased steadily since 1994, particularly on Citrus reticulata (Blanco), in'citrus-growing areas of the Western and Eastern Cape Provinces of Sou th Africa. The fecundity, body size and phenology of C. destructor were studied to provide a more informed basis for control programmes. No significant differences in fecundity were found between orchards (P > 0.05). However, fecundity varied signifi cantly between female size-classes from the same orchard (P 0.05). Ceroplustes destructor had one generation per year in the Western Cape Province, South Africa. Oviposition commences in mid November and con tinues until the end of December, with a few females ovipositing until mid January. The decline in population density of the second ins tar in February was followed by a steady increase in that of the third instar. The third instar stage extended to the end of July followed by a peak population of adults in AUgust.
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description of the stages of the white wax scale Ceroplastes destructor newstead homoptera coccidae
African Entomology, 1998Co-Authors: W M Wakgari, J H GiliomeeAbstract:The white wax-scale, Ceroplastes destructor Newstead, has recently increased in numbers and distribution in some Easy-peel (Citrus reticulata (Blanco)) orchards in the Western Cape Province of South Africa. A study was conducted to investigate its morphology and biology. Characteristics of the immature and adult females are descriptionbed and illustrated from field-collected and slide-mounted specimens. A key to the different stages is provided. The biology of C. destructor is briefly descriptionbed. Morphometric characteristics useful for separating the stages are discussed.
Yanzhou Zhang - One of the best experts on this subject based on the ideXlab platform.
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dna barcoding of common soft scales hemiptera coccoidea coccidae in china
Bulletin of Entomological Research, 2015Co-Authors: Xubo Wang, Jia Deng, J T Zhang, Qingsong Zhou, Yanzhou ZhangAbstract:The soft scales (Hemiptera: Coccoidea: Coccidae) are a group of sap-sucking plant parasites, many of which are notorious agricultural pests. The quarantine and economic importance of soft scales necessitates rapid and reliable identification of these taxa. Nucleotide sequences of the mitochondrial cytochrome c oxidase subunit I (COI) gene (barcoding region) and 28S rDNA were generated from 340 individuals of 36 common soft scales in China. Distance-based [(best match, Automated Barcode Gap Discovery (ABGD)], tree-based (neighbor-joining, Bayesian inference), Klee diagrams, and general mixed Yule coalescent (GMYC) models were used to evaluate barcoding success rates in the data set. Best match showed that COI and 28S sequences could provide 100 and 95.52% correct identification, respectively. The average interspecific divergences were 19.81% for COI data and 20.38% for 28S data, and mean intraspecific divergences were 0.56 and 0.07%, respectively. For COI data, multiple methods (ABGD, Klee, and tree-based methods) resulted in general congruence with morphological identifications. However, GMYC analysis tended to provide more molecular operational taxonomic units (MOTUs). Twelve MOTUs derived from five morphospecies (Rhodococcus sariuoni, Pulvinaria vitis, Pulvinaria aurantii, Parasaissetia nigra, and Ceroplastes rubens) were observed using the GMYC approach. In addition, tree-based methods showed that 28S sequences could be used for species-level identification (except for Ceroplastes ceriferus - Ceroplastes pseudoceriferus), even with low genetic variation (<1%). This report demonstrates the robustness of DNA barcoding for species discrimination of soft scales with two molecular markers (COI and 28S) and provides a reliable barcode library and rapid diagnostic tool for common soft scales in China.
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dna barcoding of six Ceroplastes species hemiptera coccoidea coccidae from china
Molecular Ecology Resources, 2012Co-Authors: Jun Deng, Tongxin Zhang, Chaodong Zhu, Yanzhou ZhangAbstract:Ceroplastes Gray (wax scales) is one of the genera of Coccidae, most species of which are considered to be serious economic pests. However, identification of Ceroplastes species is always difficult owing to the shortage of easily distinguishable morphological characters. Mitochondrial cytochrome c oxidase I (COI) sequences (or DNA barcodes) and the D2 expansion segments of the large subunit ribosomal RNA gene 28S were used for accurate identification of six Ceroplastes species (C. floridensis Comstock, C. japonicus Green, C. ceriferus (Fabricius), C. pseudoceriferus Green, C. rubens Maskell and C. kunmingensis Tang et Xie) from 20 different locations in China. For COI data, low G·C content was found in all species, averaging about 20.4%. Sequence divergences (K2P) between congeneric species averaged 12.19%, while intra-specific divergences averaged 0.42%. All 112 samples fell into six reciprocally monophyletic clades in the COI neighbour-joining (NJ) tree. The NJ tree inferred from 28S showed almost same results, but samples of two closely related species, C. ceriferus and C. pseudoceriferus, were clustered together. This research indicates that the standard barcode region of COI can efficiently identify similar Ceroplastes species. This study provides an example of the usefulness of barcoding for Ceroplastes identification.