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Fabrice Martin-laurent - One of the best experts on this subject based on the ideXlab platform.
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Categorizing Chlordecone potential degradation products to explore their environmental fate
Sciences of the total Environment, 2017Co-Authors: Pierre Benoit, Laure Mamy, Rémi Servien, Eric Latrille, Virginie Rossard, Fabienne Bessac, Dominique Patureau, Fabrice Martin-laurentAbstract:Chlordecone (C10Cl10O; CAS number 143-50-0) has been used extensively as an organochlorine insecticide but is nowadays banned and listed on annex A in The Stockholm Convention on Persistent Organic Pollutants (POPs). Although experimental evidences of biodegradation of this compound are scarce, several dechlorination products have been proposed by Dolfing et al. (2012) using Gibbs free energy calculations to explore different potential transformation routes. We here present the results of an in silico classification (TyPol - Typology of Pollutants) of Chlordecone transformation products (TPs) based on statistical analyses combining several environmental endpoints and structural molecular descriptors. Starting from the list of putative Chlordecone TPs and considering available data on degradation routes of other organochlorine compounds, we used different clustering strategies to explore the potential environmental behaviour of putative Chlordecone TPs from the knowledge on their molecular descriptors. The method offers the possibility to focus on TPs present in different classes and to infer their environmental fate. Thus, we have deduced some hypothetical trends for the environmental behaviour of TPs of Chlordecone assuming that TPs, which were clustered away from Chlordecone, would have different environmental fate and ecotoxicological impact compared to Chlordecone. Our findings suggest that mono- and di-hydroChlordecone, which are TPs of Chlordecone often found in contaminated soils, may have similar environmental behaviour in terms of persistence.
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Evaluation of the ecotoxicological impact of the organochlorine Chlordecone on soil microbial community structure, abundance, and function
Environmental Science and Pollution Research, 2016Co-Authors: Chloé Merlin, Marion Devers, Jérémie Béguet, Baptiste Boggio, Nadine Rouard, Fabrice Martin-laurentAbstract:The insecticide Chlordecone applied for decades in banana plantations currently contaminates 20,000 ha of arable land in the French West Indies. Although the impact of various pesticides on soil microorganisms has been studied, Chlordecone toxicity to the soil microbial community has never been assessed. We investigated in two different soils (sandy loam and silty loam) exposed to different concentrations of CLD (D0, control; D1 and D10, 1 and 10 times the agronomical dose) over different periods of time (3, 7, and 32 days): (i) the fate of Chlordecone by measuring ^14C-Chlordecone mass balance and (ii) the impact of Chlordecone on microbial community structure, abundance, and function, using standardized methods (-A-RISA, taxon-specific quantitative PCR (qPCR), and ^14C-compounds mineralizing activity). Mineralization of ^14C-Chlordecone was inferior below 1 % of initial ^14C-activity. Less than 2 % of ^14C-activity was retrieved from the water-soluble fraction, while most of it remained in the organic-solvent-extractable fraction (75 % of initial ^14C-activity). Only 23 % of the remaining ^14C-activity was measured in nonextractable fraction. The fate of Chlordecone significantly differed between the two soils. The soluble and nonextractable fractions were significantly higher in sandy loam soil than in silty loam soil. All the measured microbiological parameters allowed discriminating statistically the two soils and showed a variation over time. The genetic structure of the bacterial community remained insensitive to Chlordecone exposure in silty loam soil. In response to Chlordecone exposure, the abundance of Gram-negative bacterial groups (β-, γ-Proteobacteria, Planctomycetes, and Bacteroidetes) was significantly modified only in sandy loam soil. The mineralization of ^14C-sodium acetate and ^14C-2,4- d was insensitive to Chlordecone exposure in silty loam soil. However, mineralization of ^14C-sodium acetate was significantly reduced in soil microcosms of sandy loam soil exposed to Chlordecone as compared to the control (D0). These data show that Chlordecone exposure induced changes in microbial community taxonomic composition and function in one of the two soils, suggesting microbial toxicity of this organochlorine.
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Evaluation of the ecotoxicological impact of the organochlorine Chlordecone on soil microbial community structure, abundance, and function
Environmental Science and Pollution Research, 2016Co-Authors: Chloé Merlin, Marion Devers, Jérémie Béguet, Baptiste Boggio, Nadine Rouard, Fabrice Martin-laurentAbstract:The insecticide Chlordecone applied for decades in banana plantations currently contaminates 20,000 ha of arable land in the French West Indies. Although the impact of various pesticides on soil microorganisms has been studied, Chlordecone toxicity to the soil microbial community has never been assessed. We investigated in two different soils (sandy loam and silty loam) exposed to different concentrations of CLD (D0, control; D1 and D10, 1 and 10 times the agronomical dose) over different periods of time (3, 7, and 32 days): (i) the fate of Chlordecone by measuring C-14-Chlordecone mass balance and (ii) the impact of Chlordecone on microbial community structure, abundance, and function, using standardized methods (-A-RISA, taxon-specific quantitative PCR (qPCR), and C-14-compounds mineralizing activity). Mineralization of C-14-Chlordecone was inferior below 1 % of initial C-14-activity. Less than 2 % of C-14-activity was retrieved from the water-soluble fraction, while most of it remained in the organic-solvent-extractable fraction (75 % of initial C-14-activity). Only 23 % of the remaining C-14-activity was measured in nonextractable fraction. The fate of Chlordecone significantly differed between the two soils. The soluble and nonextractable fractions were significantly higher in sandy loam soil than in silty loam soil. All the measured microbiological parameters allowed discriminating statistically the two soils and showed a variation over time. The genetic structure of the bacterial community remained insensitive to Chlordecone exposure in silty loam soil. In response to Chlordecone exposure, the abundance of Gram-negative bacterial groups (beta-, gamma-Proteobacteria, Planctomycetes, and Bacteroidetes) was significantly modified only in sandy loam soil. The mineralization of C-14-sodium acetate and C-14-2,4-d was insensitive to Chlordecone exposure in silty loam soil. However, mineralization of C-14-sodium acetate was significantly reduced in soil microcosms of sandy loam soil exposed to Chlordecone as compared to the control (D0). These data show that Chlordecone exposure induced changes in microbial community taxonomic composition and function in one of the two soils, suggesting microbial toxicity of this organochlorine.
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Characterization of Chlordecone-tolerant fungal populations isolated from long-term polluted tropical volcanic soil in the French West Indies
Environmental Science and Pollution Research, 2014Co-Authors: Chloé Merlin, Christian Mougin, Marion Devers-lamrani, Olivier Crouzet, Cécile Héraud, Christian Steinberg, Fabrice Martin-laurentAbstract:The insecticide Chlordecone is a contaminant found in most of the banana plantations in the French West Indies. This study aims to search for fungal populations able to grow on it. An Andosol heavily contaminated with Chlordecone, perfused for 1 year in a soil–charcoal system, was used to conduct enrichment cultures. A total of 103 fungal strains able to grow on Chlordecone-mineral salt medium were isolated, purified, and deposited in the MIAE collection (Microorganismes d'Intérêt Agro-Environnemental, UMR Agroécologie, Institut National de la Recherche Agronomique, Dijon, France). Internal transcribed spacer sequencing revealed that all isolated strains belonged to the Ascomycota phylum and gathered in 11 genera: Metacordyceps, Cordyceps, Pochonia, Acremonium, Fusarium, Paecilomyces, Ophiocordyceps, Purpureocillium, Bionectria, Penicillium, and Aspergillus. Among predominant species, only one isolate, Fusarium oxysporum MIAE01197, was able to grow in a liquid culture medium that contained Chlordecone as sole carbon source. Chlordecone increased F. oxysporum MIAE01197 growth rate, attesting for its tolerance to this organochlorine. Moreover, F. oxysporum MIAE01197 exhibited a higher EC50 value than the reference strain F. oxysporum MIAE00047. This further suggests its adaptation to Chlordecone tolerance up to 29.2 mg l−1. Gas chromatography–mass spectrometry (GC-MS) analysis revealed that 40 % of Chlordecone was dissipated in F. oxysporum MIAE01197 suspension culture. No Chlordecone metabolite was detected by GC-MS. However, weak amount of 14CO2 evolved from 14C10-Chlordecone and 14C10-metabolites were observed. Sorption of 14C10-Chlordecone onto fungal biomass followed a linear relationship (r 2 = 0.99) suggesting that it may also account for Chlordecone dissipation in F. oxysporum MIAE01197 culture
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Detection and quantification of Chlordecone in contaminated soils from the French West Indies by GC-MS using the 13C10-Chlordecone stable isotope as a tracer.
Environmental science and pollution research international, 2013Co-Authors: Fabrice Martin-laurent, Chloé Merlin, Mehdi M. Sahnoun, Guy Vollmer, Markus LübkeAbstract:Chlordecone is an organochlorine insecticide that has been widely used to control banana weevil in the French West Indies. As a result of this intense use, up to 20,000 ha are contaminated by this insecticide in the French West Indies, and this causes environmental damage and health problems. A scenario of exposure was drawn by French authorities, based on land usage records. Many efforts have been made to monitor the occurrence of Chlordecone and its main metabolites using different analytical methods, including GC, GC/MS, LC/MS, and NIRS. Although these different methods allow for the detection and quantification of Chlordecone from soils, none of them estimate the bottleneck caused by extraction of this organochlorine from soils with high adsorption ability. In this study, we used 13C10-Chlordecone as a tracer to estimate Chlordecone extraction yield and to quantify Chlordecone in soil extracts based on the 13C/12C isotope dilution. We report the optimization of 13C10-Chlordecone extraction from an Andosol. The method was found to be linear from 0.118 to 43 mg kg−1 in the Andosol, with an instrumental detection limit estimated at 8.84 μg kg−1. This method showed that Chlordecone ranged from 35.4 down to 0.18 mg kg−1 in Andosol, Nitisol, Ferralsol, and Fluvisol soil types. Traces of the metabolite β-monohydroChlordecone were detected in the Andosol, Nitisol, and Ferralsol soil samples. This last result indicates that this method could be useful to monitor the fate of Chlordecone in soils of the French West Indies.
Chloé Merlin - One of the best experts on this subject based on the ideXlab platform.
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Evaluation of the ecotoxicological impact of the organochlorine Chlordecone on soil microbial community structure, abundance, and function
Environmental Science and Pollution Research, 2016Co-Authors: Chloé Merlin, Marion Devers, Jérémie Béguet, Baptiste Boggio, Nadine Rouard, Fabrice Martin-laurentAbstract:The insecticide Chlordecone applied for decades in banana plantations currently contaminates 20,000 ha of arable land in the French West Indies. Although the impact of various pesticides on soil microorganisms has been studied, Chlordecone toxicity to the soil microbial community has never been assessed. We investigated in two different soils (sandy loam and silty loam) exposed to different concentrations of CLD (D0, control; D1 and D10, 1 and 10 times the agronomical dose) over different periods of time (3, 7, and 32 days): (i) the fate of Chlordecone by measuring ^14C-Chlordecone mass balance and (ii) the impact of Chlordecone on microbial community structure, abundance, and function, using standardized methods (-A-RISA, taxon-specific quantitative PCR (qPCR), and ^14C-compounds mineralizing activity). Mineralization of ^14C-Chlordecone was inferior below 1 % of initial ^14C-activity. Less than 2 % of ^14C-activity was retrieved from the water-soluble fraction, while most of it remained in the organic-solvent-extractable fraction (75 % of initial ^14C-activity). Only 23 % of the remaining ^14C-activity was measured in nonextractable fraction. The fate of Chlordecone significantly differed between the two soils. The soluble and nonextractable fractions were significantly higher in sandy loam soil than in silty loam soil. All the measured microbiological parameters allowed discriminating statistically the two soils and showed a variation over time. The genetic structure of the bacterial community remained insensitive to Chlordecone exposure in silty loam soil. In response to Chlordecone exposure, the abundance of Gram-negative bacterial groups (β-, γ-Proteobacteria, Planctomycetes, and Bacteroidetes) was significantly modified only in sandy loam soil. The mineralization of ^14C-sodium acetate and ^14C-2,4- d was insensitive to Chlordecone exposure in silty loam soil. However, mineralization of ^14C-sodium acetate was significantly reduced in soil microcosms of sandy loam soil exposed to Chlordecone as compared to the control (D0). These data show that Chlordecone exposure induced changes in microbial community taxonomic composition and function in one of the two soils, suggesting microbial toxicity of this organochlorine.
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Evaluation of the ecotoxicological impact of the organochlorine Chlordecone on soil microbial community structure, abundance, and function
Environmental Science and Pollution Research, 2016Co-Authors: Chloé Merlin, Marion Devers, Jérémie Béguet, Baptiste Boggio, Nadine Rouard, Fabrice Martin-laurentAbstract:The insecticide Chlordecone applied for decades in banana plantations currently contaminates 20,000 ha of arable land in the French West Indies. Although the impact of various pesticides on soil microorganisms has been studied, Chlordecone toxicity to the soil microbial community has never been assessed. We investigated in two different soils (sandy loam and silty loam) exposed to different concentrations of CLD (D0, control; D1 and D10, 1 and 10 times the agronomical dose) over different periods of time (3, 7, and 32 days): (i) the fate of Chlordecone by measuring C-14-Chlordecone mass balance and (ii) the impact of Chlordecone on microbial community structure, abundance, and function, using standardized methods (-A-RISA, taxon-specific quantitative PCR (qPCR), and C-14-compounds mineralizing activity). Mineralization of C-14-Chlordecone was inferior below 1 % of initial C-14-activity. Less than 2 % of C-14-activity was retrieved from the water-soluble fraction, while most of it remained in the organic-solvent-extractable fraction (75 % of initial C-14-activity). Only 23 % of the remaining C-14-activity was measured in nonextractable fraction. The fate of Chlordecone significantly differed between the two soils. The soluble and nonextractable fractions were significantly higher in sandy loam soil than in silty loam soil. All the measured microbiological parameters allowed discriminating statistically the two soils and showed a variation over time. The genetic structure of the bacterial community remained insensitive to Chlordecone exposure in silty loam soil. In response to Chlordecone exposure, the abundance of Gram-negative bacterial groups (beta-, gamma-Proteobacteria, Planctomycetes, and Bacteroidetes) was significantly modified only in sandy loam soil. The mineralization of C-14-sodium acetate and C-14-2,4-d was insensitive to Chlordecone exposure in silty loam soil. However, mineralization of C-14-sodium acetate was significantly reduced in soil microcosms of sandy loam soil exposed to Chlordecone as compared to the control (D0). These data show that Chlordecone exposure induced changes in microbial community taxonomic composition and function in one of the two soils, suggesting microbial toxicity of this organochlorine.
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characterization of Chlordecone tolerant fungal populations isolated from long term polluted tropical volcanic soil in the french west indies
Environmental Science and Pollution Research, 2014Co-Authors: Chloé Merlin, Christian Mougin, Olivier Crouzet, Cécile Héraud, Marion Devers, Christian E W Steinberg, Fabrice MartinlaurentAbstract:The insecticideChlordecone isa contaminant found in most of the banana plantations in the French West Indies. This study aims to search for fungal populations able to grow on it. An Andosol heavily contaminated with Chlordecone, perfused for 1 year in a soil-charcoal system, was used to conduct enrichment cultures. A total of 103 fungal strains able to grow on Chlordecone-mineral salt medium were isolated, purified, and deposited in the MIAE collection (Microorganismes d'Interet Agro-Environnemental, UMR Agroecologie, Institut National de la Recherche Agronomique, Dijon, France). Internal tran- scribed spacer sequencing revealed that all isolated strains belonged to the Ascomycota phylum and gathered in 11 genera: Metacordyceps, Cordyceps, Pochonia, Acremonium, Fusarium, Paecilomyces, Ophiocordyceps, Purpureocillium, Bionectria, Penicillium ,a ndAspergillus. Among predominant species, only one isolate,Fusarium oxysporumMIAE01197, was able to grow in a liquid culture medium that contained Chlordecone as sole carbon source. Chlordecone increased F. oxysporum MIAE01197 growth rate, attesting for its tolerance to this organ- ochlorine. Moreover, F. oxysporum MIAE01197 exhibited a higher EC50 value than the reference strain F. oxysporum MIAE00047. This further suggests its adaptation to Chlordecone tolerance up to 29.2 mg l �1 . Gas chromatography-mass spec- trometry (GC-MS) analysis revealed that 40 % of Chlordecone was dissipated in F. oxysporum MIAE01197 suspension culture. No Chlordecone metabolite was detected by GC-MS. However, weak amount of 14 CO2 evolved from 14 C10-Chlordecone and 14 C10-metabolites were observed. Sorption of 14 C10-Chlordecone onto fungal biomass followed a linear relationship (r 2 =0.99) suggesting that it may also account for Chlordecone dissipation in F. oxysporum MIAE01197 culture.
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Biodechlord search for evidence for microbial degradation of Chlordecone in French West Indies contaminated soils
2014Co-Authors: Fabrice Martin, Stéphane Vuilleumier, Chloé Merlin, G. Imfeld, Timothy M Vogel, Sébastien Cecillon, W. Achouak, Pierre Benoit, Laure Mamy, Christian MouginAbstract:Objectives: Chlordecone was intensively used in the French West Indies (FWI) to control the banana weewil. Recent surveys showed that arable soils cropped with banana, representing up to 20,000 ha, are contaminated this insecticide with concentrations ranging between 0.2 and 37.4 mg kg−1. Chlordecone was classified as a persistent organic pollutant in May 2009. It is not only contaminating soil resources but also water resources where it is often detected. This insecticide is bioaccumulated in different plant and animals thereby contaminating the food chain causing an important human health. This contaminant is recalcitrant to biodegradation and very few information is available on microbial degradation of Chlordecone in soils and almost nothing in the soil of the French West Indies. In this context, the program of research Biodechlord funded by the INRA (National Institute of Agronomical Research) in the framework the National Plan for Chlordecone (PNAC), was aiming at searching for traces of Chlordecone microbial degradation in FWI soils. This project relied on a multidisciplinary approach developed by a broad scientific consortium involving researchers from CEA, Ecole Centrale Lyon, INRA, Irstea, Strasbourg and Lyon Universities. This program was made of several approaches consisting in (i) in silico analyses aiming at (ia) classifying Chlordecone and its known metabolites using the QSAR Typol in order to define hypotheses on possible degradation pathways (ib) searching for microbial genes coding deshalogenases in genomic databank that could be used to dechlorinate Chlordecone; (ii) chemical analyses aiming at (iia) optimizing approaches to quantify Chlordecone and its main metabolites in soils, (iib) identify chemical processes (photodegradation and photocatalysis) that can be used to transform Chlordecone; (iii) search for microbes tolerant to Chlordecone able to grow on it under aerobic or anaerobic conditions; (iv) characterize the impact of Chlordecone on soil microbial communities using soil metagenomics. A synthesis of the work carried out in the framework of Biodechlord will be presented. Innovative nature of the proposed topic: Very little information is available on the processes involved in the transformation of Chlordecone in the contaminated soils of the French West Indies. Regarding to the environmental and the human health problems caused by the pollutant there is an urgent to document this question. The Biodechlord project proposed to search for chemical and microbial processes possibly involved in Chlordecone transformation in the contaminated soils of the French West Indies. Among the results accumulated by the Biodechlord project, results about the isolation of microbial cultures tolerant to Chlordecone will be highlighted.
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Characterization of Chlordecone-tolerant fungal populations isolated from long-term polluted tropical volcanic soil in the French West Indies
Environmental Science and Pollution Research, 2014Co-Authors: Chloé Merlin, Christian Mougin, Marion Devers-lamrani, Olivier Crouzet, Cécile Héraud, Christian Steinberg, Fabrice Martin-laurentAbstract:The insecticide Chlordecone is a contaminant found in most of the banana plantations in the French West Indies. This study aims to search for fungal populations able to grow on it. An Andosol heavily contaminated with Chlordecone, perfused for 1 year in a soil–charcoal system, was used to conduct enrichment cultures. A total of 103 fungal strains able to grow on Chlordecone-mineral salt medium were isolated, purified, and deposited in the MIAE collection (Microorganismes d'Intérêt Agro-Environnemental, UMR Agroécologie, Institut National de la Recherche Agronomique, Dijon, France). Internal transcribed spacer sequencing revealed that all isolated strains belonged to the Ascomycota phylum and gathered in 11 genera: Metacordyceps, Cordyceps, Pochonia, Acremonium, Fusarium, Paecilomyces, Ophiocordyceps, Purpureocillium, Bionectria, Penicillium, and Aspergillus. Among predominant species, only one isolate, Fusarium oxysporum MIAE01197, was able to grow in a liquid culture medium that contained Chlordecone as sole carbon source. Chlordecone increased F. oxysporum MIAE01197 growth rate, attesting for its tolerance to this organochlorine. Moreover, F. oxysporum MIAE01197 exhibited a higher EC50 value than the reference strain F. oxysporum MIAE00047. This further suggests its adaptation to Chlordecone tolerance up to 29.2 mg l−1. Gas chromatography–mass spectrometry (GC-MS) analysis revealed that 40 % of Chlordecone was dissipated in F. oxysporum MIAE01197 suspension culture. No Chlordecone metabolite was detected by GC-MS. However, weak amount of 14CO2 evolved from 14C10-Chlordecone and 14C10-metabolites were observed. Sorption of 14C10-Chlordecone onto fungal biomass followed a linear relationship (r 2 = 0.99) suggesting that it may also account for Chlordecone dissipation in F. oxysporum MIAE01197 culture
Jean-pierre Thomé - One of the best experts on this subject based on the ideXlab platform.
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Environmental fate of Chlordecone in coastal habitats: recent studies conducted in Guadeloupe and Martinique (Lesser Antilles)
Environmental Science and Pollution Research, 2019Co-Authors: Charlotte R Dromard, Damien A. Devault, Yolande Bouchon-navaro, Soazig Lemoine, Lionel Reynal, Jean-pierre Allenou, Sébastien Cordonnier, Nathalie Tapie, Hélène Budzinski, Jean-pierre ThoméAbstract:The organochlorine pollution by Chlordecone, an insecticide spread in the past in banana plantations, is now recognized as a major ecological, economic, and social crisis in Guadeloupe and Martinique Islands. Due to its physical and chemical properties, this molecule is particularly persistent in the natural environment. Volcanic soil of Guadeloupe and Martinique contain allophanes (amorphous clays), which favor Chlordecone trapping due to their structure and physical properties. Thus, with this trapping ability, allophanes serve as a vector allowing Chlordecone to contaminate runoff waters and, finally, the sea. In the present publication, several studies recently conducted in the Lesser Antilles have been compiled in order to evaluate the desorption of Chlordecone from allophanes when arriving in the estuarine environment and to determine the transfer of Chlordecone along marine trophic food webs. The experiments showed that 20% of the initial quantity of Chlordecone was released from allophanes in estuarine conditions and 10% in the marine environment. These results could explain the high level of contamination found in the suspended organic matter and zooplankton in the coastal areas located downstream of the contaminated watersheds. The contamination of the marine food webs of mangroves, seagrass beds, and coral reefs is dominated by a contamination “by bath” in littoral waters containing Chlordecone and by bioamplification seawards.
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Environmental fate of Chlordecone in coastal habitats: recent studies conducted in Guadeloupe and Martinique (Lesser Antilles)
Environmental Science and Pollution Research, 2019Co-Authors: Charlotte R Dromard, Damien A. Devault, Yolande Bouchon-navaro, Soazig Lemoine, Lionel Reynal, Jean-pierre Allenou, Sébastien Cordonnier, Nathalie Tapie, Hélène Budzinski, Jean-pierre ThoméAbstract:The organochlorine pollution by Chlordecone, an insecticide spread in the past in banana plantations, is now recognized as a major ecological, economic and social crisis in Guadeloupe and Martinique Islands. Due to its physical and chemical properties, this molecule is particularly persistent in the natural environment. Volcanic soil of Guadeloupe and Martinique contain allophanes (amorphous clays), which favor Chlordecone trapping due to their structure and physical properties. Thus, with this trapping ability, allophanes could constitute a vector allowing Chlordecone to contaminate runoff waters, and finally the sea. In the present publication, several studies recently conducted in the Lesser Antilles have been compiled in order to evaluate the desorption of Chlordecone from allophanes when arriving in the estuarine environment and to determine the transfer of Chlordecone along marine trophic food webs. The experiments showed that 20% of the initial quantity of Chlordecone was released from allophones in estuarine conditions and 10% in marine environment. These results could explain the high level of contamination found in the suspended organic matter and zooplankton in the coastal areas located downstream the contaminated watersheds. The contamination of the marine food webs of mangroves seagrass beds and coral reefs, is dominated by a contamination 'by bath" in littoral waters containing Chlordecone and by bioamplification seawards.
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Prenatal exposure to Chlordecone, gestational weight gain, and birth weight in a Guadeloupean birth cohort.
Environmental research, 2016Co-Authors: David Hervé, Jean-pierre Thomé, Luc Multigner, Christine Monfort, Philippe Kadhel, Nathalie Costet, Florence Rouget, Sylvaine CordierAbstract:Abstract Background Chlordecone is a persistent organochlorine insecticide with well-defined estrogenic properties. It was intensively used in the French West Indies until 1993 to control the banana root borer. Because of the long-term contamination of soils and water, the population is currently exposed to Chlordecone through food consumption. Chlordecone has been found in the blood of pregnant women and in cord blood. It has been shown to be an endocrine-disrupting chemical and exposure during pregnancy may affect fetal growth. Objectives The objective of our study was to examine the association between prenatal exposure to Chlordecone and fetal growth based on the TIMOUN birth cohort conducted in Guadeloupe, with a focus on the potential modification of this relationship by maternal body mass index (BMI) and gestational weight gain (GWG). Methods Chlordecone was determined in cord plasma at birth in 593 babies. Birth weight was the indicator of fetal growth. Maternal pre-pregnancy BMI and GWG were determined. Adherence to GWG recommendations of the US Institute of Medicine based on maternal pre-pregnancy BMI was assessed. Birth weight was analyzed relative to cord blood Chlordecone levels using linear and non-linear regression models. Results Overall Chlordecone in cord blood was not associated with birth weight, but we found an interaction between Chlordecone exposure with GWG and adherence to GWG recommendations. After stratification by GWG, we found a significant U-shaped association between birth weight and Chlordecone exposure, within the upper quartiles of GWG or excessive GWG. Conclusion Chlordecone exposure may affect fetal growth, particularly when excessive GWG is present.
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vitellogenin and vitellogenin receptor gene expression and 20 hydroxyecdysone concentration in macrobrachium rosenbergii exposed to Chlordecone
Environmental Science and Pollution Research, 2016Co-Authors: Anne Lafontaine, Jean-pierre Thomé, Celine Boulangelecomte, Joelle Forgetleray, Marc Hanikenne, Eric GismondiAbstract:Chlordecone is a persistent organochlorine pesticide widely used in Guadeloupe (French West Indies) to control the banana weevil Cosmopolites sordidus. Although it was previously highlighted that Chlordecone may affect the reproduction and growth of vertebrate species, little information is available on the Chlordecone effects in invertebrates. The present study investigated the effects of Chlordecone on a hormone and a protein having key roles in reproduction and growth of the decapod crustacean Macrobrachium rosenbergii, by measuring the 20-hydroxyecdysone concentration, vitellogenin, and vitellogenin receptor gene expression, as well as the bioconcentration of Chlordecone in exposed prawns. First, the results revealed that Chlordecone was accumulated in M. rosenbergii. Then, it was found that Vg and VgR gene expression were increased in male and female M. rosenbergii exposed to Chlordecone for 90 and 240 days, while the 20-hydroxyecdysone concentrations were decreased. This work suggests that Chlordecone accumulates in prawn tissues and could affect key molecules involved in the reproduction and the growth of the invertebrate M. rosenbergii. However, many questions remain unresolved regarding the impacts of Chlordecone on growth and reproduction and the signaling pathways responsible for these effects, as well as the potential role of confounding factors present in in situ studies.
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Pesticide exposure of pregnant women in Guadeloupe: ability of a food frequency questionnaire to estimate blood concentration of Chlordecone.
Environmental Research, 2010Co-Authors: Laurence Guldner, Jean-pierre Thomé, Luc Multigner, Fanny Héraud, Christine Monfort, Arnaud Giusti, Philippe Kadhel, Sylvaine CordierAbstract:CONTEXT: Chlordecone, an environmentally persistent organochlorine insecticide used intensively in banana culture in the French West Indies until 1993, has permanently polluted soils and contaminated foodstuffs. Consumption of contaminated food is the main source of exposure nowadays. We sought to identify main contributors to blood Chlordecone concentration (BCC) and to validate an exposure indicator based on food intakes. MATERIAL AND METHODS: We used a food frequency questionnaire (FFQ) completed by a sample of 194 pregnant women to estimate their dietary exposure to Chlordecone and compared it to blood levels. In a first approach, Chlordecone daily intake was estimated as the product of daily eaten quantity of 214 foodstuffs, multiplied by their Chlordecone content, and summed over all items. We then predicted individual blood Chlordecone concentration with empirical weight regression models based on frequency of food consumption, and without contamination data. RESULTS: Among the 191 subjects who had BCC determination, 146 (76%) had detectable values and mean BCC was 0.86 ng/mL (range < LOD-13.2). Mean per capita dietary intake of Chlordecone was estimated at 3.3 microg/day (range: 0.1-22.2). Blood Chlordecone levels were significantly correlated with food exposure predicted from the empirical weight models (r=0.47, p
Thierry Woignier - One of the best experts on this subject based on the ideXlab platform.
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soil microstructure and organic matter keys for Chlordecone sequestration
Journal of Hazardous Materials, 2013Co-Authors: Thierry Woignier, Paula Fernandes, Florence Clostre, Alain Soler, Luc Rangon, Céline Carles, Magalie LesueurjannoyerAbstract:Past applications of Chlordecone, a persistent organochlorine pesticide, have resulted in diffuse pollution of agricultural soils, and these have become sources of contamination of cultivated crops as well as terrestrial and marine ecosystems. Chlordecone is a very stable and recalcitrant molecule, mainly present in the solid phase, and has a strong affinity for organic matter. To prevent consumer and ecosystem exposure, factors that influence Chlordecone migration in the environment need to be evaluated. In this study, we measured the impact of incorporating compost on Chlordecone sequestration in andosols as a possible way to reduce plant contamination. We first characterized the transfer of Chlordecone from soil to plants (radish, cucumber, and lettuce). Two months after incorporation of the compost, soil-plant transfers were reduced by a factor of 1.9-15 depending on the crop. Our results showed that adding compost modified the fractal microstructure of allophane clays thus favoring Chlordecone retention in andosols. The complex structure of allophane and the associated low accessibility are important characteristics governing the fate of Chlordecone. These results support our proposal for an alternative strategy that is quite the opposite of total soil decontamination: Chlordecone sequestration.
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Soil microstructure and organic matter: Keys for Chlordecone sequestration ☆
Journal of hazardous materials, 2013Co-Authors: Thierry Woignier, Paula Fernandes, Florence Clostre, Alain Soler, Luc Rangon, Céline Carles, Magalie Lesueur-jannoyerAbstract:Past applications of Chlordecone, a persistent organochlorine pesticide, have resulted in diffuse pollution of agricultural soils, and these have become sources of contamination of cultivated crops as well as terrestrial and marine ecosystems. Chlordecone is a very stable and recalcitrant molecule, mainly present in the solid phase, and has a strong affinity for organic matter. To prevent consumer and ecosystem exposure, factors that influence Chlordecone migration in the environment need to be evaluated. In this study, we measured the impact of incorporating compost on Chlordecone sequestration in andosols as a possible way to reduce plant contamination. We first characterized the transfer of Chlordecone from soil to plants (radish, cucumber, and lettuce). Two months after incorporation of the compost, soil-plant transfers were reduced by a factor of 1.9-15 depending on the crop. Our results showed that adding compost modified the fractal microstructure of allophane clays thus favoring Chlordecone retention in andosols. The complex structure of allophane and the associated low accessibility are important characteristics governing the fate of Chlordecone. These results support our proposal for an alternative strategy that is quite the opposite of total soil decontamination: Chlordecone sequestration.
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Chlordecone retention in the fractal structure of volcanic clay.
Journal of hazardous materials, 2012Co-Authors: Thierry Woignier, Florence Clostre, Hervé Macarie, Magalie JannoyerAbstract:Chlordecone (CHLD), a soil and foodstuff pollutant, as well as an environmentally persistent organochlorine insecticide, was used intensively in banana fields. The Chlordecone uptake of three crops was measured for two types of polluted soils: allophanic and non-allophanic. The uptake is lower for allophanic soils even if their Chlordecone content is higher than with non-allophanic soils. The fractal structure of the allophane aggregates was characterized at the nanoscale by small angle X-rays scattering, pore size distribution and transmission electron microscopy. We showed that clay microstructures should be an important physico-chemical factor governing the fate of Chlordecone in the environment. Allophanic clays result in two counterintuitive findings: higher contaminant trappings yet lower contaminant availability. We propose that this specific, tortuous structure, along with its associated low accessibility, partly explains the low availability of Chlordecone confined in allophanic soils. Capsule The fractal and tortuous microstructure of allophane clay favours the Chlordecone retention in soils and disfavours the crop uptake.
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Sequestration of Chlordecone in the porous structure of an andosol and effects of added organic matter: an alternative to decontamination
European Journal of Soil Science, 2012Co-Authors: Thierry Woignier, Paula Fernandes, Magalie Jannoyer-lesueur, Alain SolerAbstract:The context of this study is the pollution of soils and water by a persistent organo-chlorinated insecticide, Chlordecone, in a tropical environment. The application of Chlordecone to control the banana black weevil has led to continuing diffuse pollution of soils, and to its being a source of contamination for cultivated plants, as well as for terrestrial and marine ecosystems. Chlordecone is toxic and stable and is considered to be a persistent organic pesticide. Consequently, the amounts of Chlordecone that could migrate through the environment and contaminate agricultural products need to be controlled. We measured the impact of two composts (5% weight) on Chlordecone sequestration in andosols. To this end, we first characterized the transfer of Chlordecone from soil to water, and then its transfer from soil to plants. After 3 months of maturation, soil-water and soil-plant transfers were reduced by a factor of from 3 to 10. We also showed that adding compost to contaminated soils increases Chlordecone sequestration because it leads to changes in soil microstructure in the form of pore collapse and closure of the fractal structure of the allophane content. (Resume d'auteur)
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Soil microstructure and added organic matter: keys for Chlordecone sequestration
2012Co-Authors: Paula Fernandes, Magalie Jannoyer-lesueur, Florence Clostre, Alain Soler, Luc Rangon, Thierry WoignierAbstract:The scientific and economic context of our study is related to the pollution of the soils, fresh and marine water by a persistent organochlorine pesticide (Chlordecone) in a tropical context. The former application of Chlordecone results today in a diffuse pollution in agricultural soils, which are sources of contamination for cultivated roots, tubers, vegetables and terrestrial and marine ecosystems. Chlordecone is a very though and stable molecule (considered as a POP), it is mainly present in solid phase and has a strong affinity with organic matters. To prevent consumers and ecosystems exposure, it is thus necessary for us to evaluate the factors that influence Chlordecone migration in the environment. In our research, we studied the impacts of clay microstructure on the Chlordecone retention. We showed that allophane aggregates had a great ability to trap Chlordecone mainly due to their fractal structure. We also measured the effects of added composts on Chlordecone lability and transfer rate from soil to plant . We show that compost addition modifies the fractal structure of allophane clays favouring the Chlordecone retention. These results allow us to propose a new strategy in opposition to the complete soil decontamination: the Chlordecone sequestration. ( Resume d'auteur)
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Detection and quantification of Chlordecone in contaminated soils from the French West Indies by GC-MS using the C-13(10)-Chlordecone stable isotope as a tracer
Environmental Science and Pollution Research, 2014Co-Authors: Fabrice Martin, Chloé Merlin, Mehdi M. Sahnoun, Guy Vollmer, Markus LübkeAbstract:Chlordecone is an organochlorine insecticide that has been widely used to control banana weevil in the French West Indies. As a result of this intense use, up to 20,000 ha are contaminated by this insecticide in the French West Indies, and this causes environmental damage and health problems. A scenario of exposure was drawn by French authorities, based on land usage records. Many efforts have been made to monitor the occurrence of Chlordecone and its main metabolites using different analytical methods, including GC, GC/MS, LC/MS, and NIRS. Although these different methods allow for the detection and quantification of Chlordecone from soils, none of them estimate the bottleneck caused by extraction of this organochlorine from soils with high adsorption ability. In this study, we used C-13(10)-Chlordecone as a tracer to estimate Chlordecone extraction yield and to quantify Chlordecone in soil extracts based on the C-13/C-12 isotope dilution. We report the optimization of C-13(10)-Chlordecone extraction from an Andosol. The method was found to be linear from 0.118 to 43 mg kg(-1) in the Andosol, with an instrumental detection limit estimated at 8.84 mu g kg(-1). This method showed that Chlordecone ranged from 35.4 down to 0.18 mg kg(-1) in Andosol, Nitisol, Ferralsol, and Fluvisol soil types. Traces of the metabolite beta-monohydroChlordecone were detected in the Andosol, Nitisol, and Ferralsol soil samples. This last result indicates that this method could be useful to monitor the fate of Chlordecone in soils of the French West Indies.
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Detection and quantification of Chlordecone in contaminated soils from the French West Indies by GC-MS using the 13C10-Chlordecone stable isotope as a tracer.
Environmental science and pollution research international, 2013Co-Authors: Fabrice Martin-laurent, Chloé Merlin, Mehdi M. Sahnoun, Guy Vollmer, Markus LübkeAbstract:Chlordecone is an organochlorine insecticide that has been widely used to control banana weevil in the French West Indies. As a result of this intense use, up to 20,000 ha are contaminated by this insecticide in the French West Indies, and this causes environmental damage and health problems. A scenario of exposure was drawn by French authorities, based on land usage records. Many efforts have been made to monitor the occurrence of Chlordecone and its main metabolites using different analytical methods, including GC, GC/MS, LC/MS, and NIRS. Although these different methods allow for the detection and quantification of Chlordecone from soils, none of them estimate the bottleneck caused by extraction of this organochlorine from soils with high adsorption ability. In this study, we used 13C10-Chlordecone as a tracer to estimate Chlordecone extraction yield and to quantify Chlordecone in soil extracts based on the 13C/12C isotope dilution. We report the optimization of 13C10-Chlordecone extraction from an Andosol. The method was found to be linear from 0.118 to 43 mg kg−1 in the Andosol, with an instrumental detection limit estimated at 8.84 μg kg−1. This method showed that Chlordecone ranged from 35.4 down to 0.18 mg kg−1 in Andosol, Nitisol, Ferralsol, and Fluvisol soil types. Traces of the metabolite β-monohydroChlordecone were detected in the Andosol, Nitisol, and Ferralsol soil samples. This last result indicates that this method could be useful to monitor the fate of Chlordecone in soils of the French West Indies.