The Experts below are selected from a list of 234 Experts worldwide ranked by ideXlab platform
Mohd Yazid Abdul Manap - One of the best experts on this subject based on the ideXlab platform.
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purification of serine protease from mango mangifera indica cv chokanan peel using an alcohol salt aqueous two phase system
Food Chemistry, 2012Co-Authors: Mehrnoush Amid, M Shuhaimi, Md Zaidul Islam Sarker, Mohd Yazid Abdul ManapAbstract:An alcohol/salt-based aqueous two-phase (ATPS) system, as a novel method of purification, was employed to purify serine proteases from mango (Mangifera Indica Cv. Chokanan) peel. The effectiveness of different parameters, such as type and concentration of alcohol (1-propanol, 2-propanol, and ethanol), type of salt (sodium Citrate, Potassium phosphate, and ammonium sulphate), pH, and NaCl, on the purification and selective separation of serine protease was investigated. Desirable conditions of partition coefficient (K), selectivity (S), purification factor (P), and yield (Y%) of serine protease, using ATPS, were determined. The highest partition coefficient (64.5) and selectivity (343.2) for serine protease purification value were achieved in an ATPS of 16% (w/w) 2-propanaol, 19% (w/w) Potassium phosphate, and 5% (w/v) NaCl at pH 7.5. It was demonstrated that serine protease could be recovered with a yield of 96.7% and a purification factor of 11.6.
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Purification of serine protease from mango (Mangifera Indica Cv. Chokanan) peel using an alcohol/salt aqueous two phase system
Food chemistry, 2011Co-Authors: Mehrnoush Amid, M Shuhaimi, Zaidul Islam Sarker, Mohd Yazid Abdul ManapAbstract:An alcohol/salt-based aqueous two-phase (ATPS) system, as a novel method of purification, was employed to purify serine proteases from mango (Mangifera Indica Cv. Chokanan) peel. The effectiveness of different parameters, such as type and concentration of alcohol (1-propanol, 2-propanol, and ethanol), type of salt (sodium Citrate, Potassium phosphate, and ammonium sulphate), pH, and NaCl, on the purification and selective separation of serine protease was investigated. Desirable conditions of partition coefficient (K), selectivity (S), purification factor (P), and yield (Y%) of serine protease, using ATPS, were determined. The highest partition coefficient (64.5) and selectivity (343.2) for serine protease purification value were achieved in an ATPS of 16% (w/w) 2-propanaol, 19% (w/w) Potassium phosphate, and 5% (w/v) NaCl at pH 7.5. It was demonstrated that serine protease could be recovered with a yield of 96.7% and a purification factor of 11.6.
Fei Yang - One of the best experts on this subject based on the ideXlab platform.
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Process for coating of reconstituted tobacco sheet with Citrates
Journal of Analytical and Applied Pyrolysis, 2015Co-Authors: Wenhua Gao, Kefu Chen, Rendang Yang, Fei YangAbstract:Abstract The combustion property and thermal pyrolysis behavior of reconstituted tobacco sheet (RTS) with combustion improvers added to the RTS coatings were studied. The RTS coatings comprised of water-soluble extracts from tobacco stems and leaves, carboxymethyl cellulose and sodium Citrate/Potassium Citrate. The coating was mixed and stirred by a high-speed dispersion machine for 40 min, and then coated on the RTS base paper by papermaking method. Effects of Citrates on the properties of RTS, such as major chemical compositions, surface microstructure, tar and carbon monoxide (CO) release per cigarette were investigated. The results showed that adding sodium Citrate and Potassium Citrate in the coatings did not affect the main chemical contents and surface microstructure of RTS. With the ratio of Potassium Citrate increasing in the coating, the amount of tar and CO release per cigarette decreased significantly. Thermogravimetric (TG) analysis and the corresponding derivative thermogravimetric analysis (DTG) indicated that, compared to sodium Citrated, Potassium Citrate accelerated the thermal degradation of RTS between 200 and 400 °C, and decreased the activation energy ( E ) according to the kinetic analysis results of Coats–Redfern model. Therefore, Potassium Citrate may become a potential burning additive controlling CO and tar release in mainstream smoke of RTS.
Liusi Sheng - One of the best experts on this subject based on the ideXlab platform.
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Effect of Potassium organic and inorganic salts on thermal decomposition of reconstituted tobacco sheet
Journal of Thermal Analysis and Calorimetry, 2017Co-Authors: Mengmeng Ding, Bin Wei, Zhao Zhang, She Shike, Huang Lan, Liusi ShengAbstract:In order to deeply investigate the role of Potassium in modifying thermal behavior and burning characteristics of reconstituted tobacco sheet (RTS), electrodialysis (ED) technology was employed at first to remove the main ions in the tobacco extract to reduce interference of endogenous ions. Characterization on thermal behavior of RTS treated with three organic (acetate, malate and Citrate) Potassium salts and three inorganic (chloride, sulfate and phosphate) Potassium salts was then performed using thermogravimetric analyzer coupled with Fourier transforms infrared spectrometer (TG-FTIR) at the heating rate of 20 °C min−1. TG results illustrated that Potassium decreased the peak temperature of carbohydrate pyrolysis, retarded the thermal degradation of the major components of RTS and meanwhile promoted the formation of stable char firmly depending on its chemical state. The organic Potassium salts had a more significant effect on the process of char oxidation than inorganic ones with a new peak at around 570 °C. Analyses of evolved gaseous products distribution demonstrated a significant reduction of CO and CO2 during carbohydrates pyrolysis in the presence of Potassium, especially organic Potassium salts. Moreover, the maximum firecone temperature was also measured by Infrared thermography, and it had been found that the burning cone temperature of ED-modified RTS was averagely 13.27 °C higher than that of organic Potassium treated RTS but averagely 16.57 °C lower than that of inorganic ones.
Charles Y.c. Pak - One of the best experts on this subject based on the ideXlab platform.
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Bioavailability of Potassium and magnesium, and citraturic response from Potassium-magnesium Citrate
The Journal of Urology, 1991Co-Authors: Karl Koenig, Paulette Padalino, George Alexandrides, Charles Y.c. PakAbstract:AbstractThe bioavailability of Potassium and magnesium, and the citraturic response were determined for the new compound, Potassium-magnesium Citrate, in 14 normal volunteers. Results were compared to those of Potassium Citrate and magnesium Citrate. Each subject participated in 4 phases of study: Potassium-magnesium Citrate, Potassium Citrate, magnesium Citrate and Potassium chloride. After stabilization on a metabolic diet, each subject ingested a single load of a test medication followed by timed urine collections for the next 24 hours. Test loads included Potassium-magnesium Citrate (49mEq. Potassium, 24.5mEq. magnesium and 73.5mEq. Citrate), Potassium Citrate (50mEq.), Potassium chloride (50mEq.) and magnesium Citrate (25mEq.). Urinary Potassium, magnesium and Citrate were measured for each collection period.Potassium-magnesium Citrate provided an equivalent Potassium bioavailability as Potassium Citrate and Potassium chloride, and a comparable magnesium bioavailability as magnesium Citrate. However,...
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Contrasting effects of various Potassium salts on renal Citrate excretion.
The Journal of clinical endocrinology and metabolism, 1991Co-Authors: Khashayar Sakhaee, Harry R. Jacobson, Robert J. Alpern, Charles Y.c. PakAbstract:Mechanisms for the citraturic response to Potassium Citrate treatment were sought by assessing renal Citrate clearance and acid-base status after oral administration of Potassium Citrate, Potassium bicarbonate, and Potassium chloride. After 2 weeks of treatment of eight patients with stones at a dose of 80 meq/day, urinary Citrate rose significantly from 2.5 +/- 1.6 mmol/day (no drug) to 5.1 +/- 1.7 mmol/day with Potassium Citrate and to 4.5 +/- 1.5 mmol/day with Potassium bicarbonate (P less than 0.05), but did not change significantly with Potassium chloride. Citrate clearance increased from 8.0 to 27.4 mL/min with Potassium Citrate and 25.8 mL/min with Potassium bicarbonate (P less than 0.05), but did not increase with Potassium chloride. Both Potassium Citrate and Potassium bicarbonate significantly raised urinary bicarbonate and decreased urinary ammonium, titratable acid, and net acid excretion. Potassium chloride was without effect. Effects of Potassium Citrate on urinary Citrate, Citrate clearance, and acid-base status tended to be more prominent than those of Potassium bicarbonate, but these changes were not significant. Thus, the citraturic action of Potassium Citrate is largely accountable for by provision of an alkali load. Potassium itself had no effect in the absence of Potassium deficiency.
Harry R. Jacobson - One of the best experts on this subject based on the ideXlab platform.
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Contrasting effects of various Potassium salts on renal Citrate excretion.
The Journal of Clinical Endocrinology and Metabolism, 1991Co-Authors: Khashayar Sakhaee, Robert J. Alpern, Harry R. JacobsonAbstract:Mechanisms for the citraturic response to Potassium Citrate treatment were sought by assessing renal Citrate clearance and acid-base status after oral administration of Potassium Citrate, Potassium bicarbonate, and Potassium chloride. After 2 weeks of treatment of eight patients with stones at a dose of 80 meq/day, urinary Citrate rose significantly from 2.5 ± 1.6 mmol/day (no drug) to 5.1 ± 1.7 mmol/day with Potassium Citrate and to 4.5 ± 1.5 mmol/day with Potassium bicarbonate (P < 0.05), but did not change significantly with Potassium chloride. Citrate clearance increased from 8.0 to 27.4 mL/min with Potassium Citrate and 25.8 mL/min with Potassium bicarbonate (P < 0.05), but did not ir. crease with Potassium chloride. Both Potassium Citrate and Potassium bicarbonate significantly raised urinary bicarbonate and decreased urinary ammonium, titratable acid, and net acid excretion. Potassium chloride was without effect. Effects of Potassium Citrate on urinary Citrate, Citrate clearance, and acid-base stat...
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Contrasting effects of various Potassium salts on renal Citrate excretion.
The Journal of clinical endocrinology and metabolism, 1991Co-Authors: Khashayar Sakhaee, Harry R. Jacobson, Robert J. Alpern, Charles Y.c. PakAbstract:Mechanisms for the citraturic response to Potassium Citrate treatment were sought by assessing renal Citrate clearance and acid-base status after oral administration of Potassium Citrate, Potassium bicarbonate, and Potassium chloride. After 2 weeks of treatment of eight patients with stones at a dose of 80 meq/day, urinary Citrate rose significantly from 2.5 +/- 1.6 mmol/day (no drug) to 5.1 +/- 1.7 mmol/day with Potassium Citrate and to 4.5 +/- 1.5 mmol/day with Potassium bicarbonate (P less than 0.05), but did not change significantly with Potassium chloride. Citrate clearance increased from 8.0 to 27.4 mL/min with Potassium Citrate and 25.8 mL/min with Potassium bicarbonate (P less than 0.05), but did not increase with Potassium chloride. Both Potassium Citrate and Potassium bicarbonate significantly raised urinary bicarbonate and decreased urinary ammonium, titratable acid, and net acid excretion. Potassium chloride was without effect. Effects of Potassium Citrate on urinary Citrate, Citrate clearance, and acid-base status tended to be more prominent than those of Potassium bicarbonate, but these changes were not significant. Thus, the citraturic action of Potassium Citrate is largely accountable for by provision of an alkali load. Potassium itself had no effect in the absence of Potassium deficiency.