The Experts below are selected from a list of 303 Experts worldwide ranked by ideXlab platform

Stefano Guerra - One of the best experts on this subject based on the ideXlab platform.

  • CC16 Levels into Adult Life Are Associated with Nitrogen Dioxide Exposure at Birth.
    American Journal of Respiratory and Critical Care Medicine, 2019
    Co-Authors: Paloma I. Beamer, Jing Zhai, Debra A Stern, Stefano Guerra, Marilyn Halonen, Melissa Furlong, Nathan Lothrop, Dean Billheimer, Anne L. Wright, Fernando D Martinez
    Abstract:

    Rationale: Lung function and growth are adversely associated with nitrogen dioxide (NO2) exposure. Lower levels of circulating Club Cell secretory protein (CC16) in childhood are also associated wi...

  • serum Club Cell protein 16 is associated with asymptomatic airway responsiveness in adults findings from the french epidemiological study on the genetics and environment of asthma
    Respirology, 2015
    Co-Authors: Marta Rava, Alfred Bernard, Stefano Guerra, Nicole Le Moual, Xavier Dumont, Valerie Siroux, Benedicte Jacquemin, Francine Kauffmann, Rachel Nadif
    Abstract:

    Club Cell secretory protein (CC-16) is a sensitive biomarker of airways epithelium integrity. It has gained interest as a biological marker in chronic lung diseases because of its presumed relationship to inflammation. Little is known about the association between CC-16 serum level and asthma, lung function and airway responsiveness (AR). Serum CC-16 level was determined by latex immunoassay in 1298 participants from the French Epidemiological case-control and family-based study on Genetics and Environment of Asthma (EGEA) (mean age 43 years; 49% men, 38% with asthma). Pre-bronchodilator lung function (forced expiratory volume in 1 s (FEV1 ), forced vital capacity (FVC) and FEV1 /FVC) and degree of AR, expressed as a function of the dose-response slope to methacholine test were measured. Standardized residuals CC-16 z-scores were obtained by regressing CC-16 level on the glomerular filtration rate. CC-16 z-scores were correlated with asthma, lung function and AR in participants with and without asthma. CC-16 geometric mean level was 12.4 μg/L (range: 2.2-70.6 μg/L). In participants without asthma, lower CC-16 z-scores was associated with impaired FEV1 /FVC% (β = 0.50 (95% CI: 0.06, 0.95) and with higher degree of AR (β = 0.24 (95% CI: 0.09, 0.39)). CC-16 was not associated with impaired lung function or AR in participants with asthma. Lower CC-16 serum level was associated with impaired lung function and AR, suggesting that serum CC-16 level may reflect early damages to the lung epithelium in adults without asthma.

  • serum concentrations of Club Cell secretory protein clara and cancer mortality in adults a population based prospective cohort study
    The Lancet Respiratory Medicine, 2013
    Co-Authors: Stefano Guerra, Monica M Vasquez, Amber Spangenberg, Marilyn Halonen, Fernando D Martinez
    Abstract:

    Summary Background Club Cell secretory protein (Clara) (CC16) is produced mainly by bronchiolar Club Cells and has been shown to have protective effects against airway inflammation and oxidative stress from cigarette smoking and related carcinogens. The goal of this study was to establish whether serum CC16 concentrations predict all-cause and cancer-specific mortality in adults. Methods We used data from the population-based Tucson Epidemiological Study of Airway Obstructive Diseases (TESAOD), a prospective cohort study of respiratory health initiated in Tucson, AZ, USA, in 1972, that recruited a multistage stratified cluster sample of non-Hispanic white households. We measured serum CC16 concentrations in cryopreserved serum samples and reviewed vital status up to Jan 1, 2011, through contact with next of kin, collection of death certificates, and searches of the National Death Index. Our primary analysis was the relation of baseline serum CC16 to all-cause mortality or cause-specific mortality risk, analysed by adjusted Cox proportional hazards models. Findings 1086 TESAOD participants aged 21–70 years at enrolment were eligible for inclusion. Of these, 653 (60%) had died by 2011, and cause of death was ascertained for 649 (99%). When adjusted for sex, age, education, body-mass index, smoking and pack-years, and baseline levels of lung function, serum CC16 concentrations at baseline were inversely associated with mortality risk over the study follow-up. Mortality risk increased for each 1-SD decrease in CC16 (adjusted hazard ratio [HR] 1·16 [95% CI 1·06–1·26]; p=0·0007). For cause-specific mortality, each 1-SD decrease in serum CC16 was associated with an increased risk of dying of cancer (adjusted HR 1·41 [1·19–1·67]; p Interpretation Serum CC16 concentrations can predict mortality risk in the general adult population. The excess risk associated with lower CC16 concentrations is predominantly driven by cancer, particularly lung cancer. Funding National Heart, Lung, and Blood Institute.

Ellen Tufvesson - One of the best experts on this subject based on the ideXlab platform.

  • Club Cell secretory protein cc16 in gastric fluid at birth and subsequent lung disease in preterm infants
    Pediatric Pulmonology, 2018
    Co-Authors: Cecilia Hagman, Ellen Tufvesson, Lars J Bjorklund, Gunnel Hellgren, Ingrid Hansenpupp
    Abstract:

    Background: Club Cell secretory protein (CC16) probably has a role in protecting the lung from inflammation. Aim: To evaluate if low levels of CC16 in gastric fluid at birth, reflecting low levels of CC16 in the lung, would be associated with lung inflammation and respiratory morbidity. Methods: A study of 64 infants with mean gestational age 26.1 weeks. CC16 was analyzed in gastric fluid at birth. CC16, pro-inflammatory cytokines, and MMP-9 were analyzed in tracheal aspirate within 24 h from birth. Results: CC16 in gastric fluid increased with gestational age (P = 0.033). Lower concentrations of CC16 in gastric fluid at birth were associated with higher concentrations of IL-1β (P = 0.028), TNF-α (P = 0.034), and MMP-9 (P = 0.015) in tracheal aspirate. Infants who needed mechanical ventilation at 24 and 72 h of age had lower CC16 in gastric fluid than those not ventilated at these ages (P = 0.011 and P = 0.024, respectively). Lower CC16 in gastric fluid was associated with higher FiO2 at 6 h (P = 0.009), higher PaCO2 at 24 h (P = 0.03), more ventilator days (P = 0.012) and more days with supplemental oxygen (P = 0.03). Infants who had either died or were still treated with supplemental oxygen at 36 weeks postmenstrual age had lower CC16 in gastric fluid than infants with none of these outcomes (P = 0.049). Conclusion: A low CC16 concentration in gastric fluid at birth was associated with increased inflammation in the trachea within the first 24 h of life and with more need for respiratory support in the neonatal period. (Less)

  • Club Cell protein cc16 in plasma bronchial brushes bal and urine following an inhaled allergen challenge in allergic asthmatics
    Biomarkers, 2017
    Co-Authors: Henning Stenberg, Leif Bjermer, Erik Wadelius, Subhabrata Moitra, Ida Aberg, Jaro Ankerst, Zuzana Diamant, Ellen Tufvesson
    Abstract:

    Background: Club Cell protein (CC16) is a pneumoprotein secreted by epithelial Club Cells. CC16 possesses anti-inflammatory properties and is a potential biomarker for airway epithelial damage. We studied the effect of inhaled allergen on pulmonary and systemic CC16 levels. Methods: Thirty-four subjects with allergic asthma underwent an inhaled allergen challenge. Bronchoscopy with bronchoalveolar lavage (BAL) and brushings was performed before and 24 h after the challenge. CC16 was quantified in BAL and CC16 positive Cells and CC16 mRNA in bronchial brushings. CC16 was measured in plasma and urine before and repeatedly after the challenge. Thirty subjects performed a mannitol inhalation challenge prior to the allergen challenge. Results: Compared to baseline, CC16 in plasma was significantly increased in all subjects 0-1 h after the allergen challenge, while CC16 in BAL was only increased in subjects without a late allergic response. Levels of CC16 in plasma and in the alveolar fraction of BAL correlated significantly after the challenge. There was no increase in urinary levels of CC16 post-challenge. Mannitol responsiveness was greater in subjects with lower baseline levels of CC16 in plasma. Conclusions: The increase in plasma CC16 following inhaled allergen supports the notion of CC16 as a biomarker of epithelial dysfunction.

  • effect of terbutaline on hyperpnoea induced bronchoconstriction and urinary Club Cell protein 16 in athletes
    Journal of Applied Physiology, 2013
    Co-Authors: Andrew J. Simpson, Ellen Tufvesson, Sandra D. Anderson, Lee M. Romer, Leif Bjermer, Pascale Kippelen
    Abstract:

    Repeated injury of the airway epithelium caused by hyperpnoea of poorly conditioned air has been proposed as a key factor in the pathogenesis of exercise-induced bronchoconstriction (EIB) in athletes. In animals, the short-acting beta(2)-agonist terbutaline has been shown to reduce dry airflow-induced bronchoconstriction and the associated shedding of airway epithelial Cells. Our aim was to test the efficacy of inhaled terbutaline in attenuating hyperpnoea-induced bronchoconstriction and airway epithelial injury in athletes. Twenty-seven athletes with EIB participated in a randomized, double-blind, placebo-controlled, crossover study. Athletes completed an 8-min eucapnic voluntary hyperpnoea (EVH) test with dry air on two separate days 15 min after inhaling 0.5 mg terbutaline or a matching placebo. Forced expiratory volume in 1 s (FEV1) and urinary concentration of the Club Cell (Clara Cell) protein 16 (CC16, a marker of airway epithelial perturbation) were measured before and up to 60 min after EVH. The maximum fall in FEV1 of 17 +/- 8% (SD) on placebo was reduced to 8 +/- 5% following terbutaline (P < 0.001). Terbutaline gave bronchoprotection (i.e., post-EVH FEV1 fall <10%) to 22 (81%) athletes. EVH caused an increase in urinary excretion of CC16 in both conditions (P < 0.001), and terbutaline significantly reduced this rise (pre- to postchallenge CC16 increase 416 +/- 495 pg/mu mol creatinine after placebo vs. 315 +/- 523 pg/mu mol creatinine after terbutaline, P = 0.016). These results suggest that the inhalation of a single therapeutic dose of terbutaline offers significant protection against hyperpnoea-induced bronchoconstriction and attenuates acute airway epithelial perturbation in athletes. (Less)

  • increase of Club Cell clara protein cc16 in plasma and urine after exercise challenge in asthmatics and healthy controls and correlations to exhaled breath temperature and exhaled nitric oxide
    Respiratory Medicine, 2013
    Co-Authors: Ellen Tufvesson, Jaro Ankerst, Henning Svensson, Leif Bjermer
    Abstract:

    Exercise is known to affect the airway epithelium through dehydration, followed by a release of mediators, such as Club Cell (Clara) protein (CC16). The aim of this study was to follow the CC16 levels at repeated time points in plasma and urine after exercise in asthmatic subjects and controls, and to relate the findings to exhaled breath temperature (EBT) and exhaled nitric oxide (NO). Twenty-two asthmatics and 18 healthy subjects performed an exercise challenge test on a treadmill. Lung function, CC16 in plasma and urine, EBT and fractional exhaled NO were investigated before and repeatedly for 60 min after the exercise. The increase in CC16 concentration in plasma was seen already one minute after exercise (p < 0.001) and increased further after 20 (p = 0.009) until 60 min (p = 0.001). An increase in urinary levels of CC16 peaked after 30 min (p < 0.001), and declined after 60 min but were still higher than baseline (p = 0.002). There were no differences in plasma or urine CC16 levels between asthmatics and controls, but males had higher plasma levels compared to females (p < 0.001) at all time points. EBT peaked at 15 min (p < 0.001) and thereafter declined, and FENO50 (p < 0.0001), alveolar NO concentration (p = 0.049) and bronchial flux of NO (p = 0.0055) decreased after exercise. In conclusion, this study shows that CC16 in plasma increased during 60 min after exercise, not synchronized with CC16 levels in urine. CC16 levels in plasma correlated to EBT and exhaled NO, reflecting an overall epithelial involvement. There was no difference between asthmatics and healthy controls, showing a physiological rather than pathophysiological response. (Less)

  • Effect of terbutaline on hyperpnoea-induced bronchoconstriction and urinary Club Cell protein 16 in athletes.
    Journal of Applied Physiology, 2013
    Co-Authors: Andrew J. Simpson, Ellen Tufvesson, Sandra D. Anderson, Lee M. Romer, Leif Bjermer, Pascale Kippelen
    Abstract:

    Repeated injury of the airway epithelium caused by hyperpnoea of poorly conditioned air has been proposed as a key factor in the pathogenesis of exercise-induced bronchoconstriction (EIB) in athletes. In animals, the short-acting beta(2)-agonist terbutaline has been shown to reduce dry airflow-induced bronchoconstriction and the associated shedding of airway epithelial Cells. Our aim was to test the efficacy of inhaled terbutaline in attenuating hyperpnoea-induced bronchoconstriction and airway epithelial injury in athletes. Twenty-seven athletes with EIB participated in a randomized, double-blind, placebo-controlled, crossover study. Athletes completed an 8-min eucapnic voluntary hyperpnoea (EVH) test with dry air on two separate days 15 min after inhaling 0.5 mg terbutaline or a matching placebo. Forced expiratory volume in 1 s (FEV1) and urinary concentration of the Club Cell (Clara Cell) protein 16 (CC16, a marker of airway epithelial perturbation) were measured before and up to 60 min after EVH. The maximum fall in FEV1 of 17 +/- 8% (SD) on placebo was reduced to 8 +/- 5% following terbutaline (P < 0.001). Terbutaline gave bronchoprotection (i.e., post-EVH FEV1 fall

Arnaud Bourdin - One of the best experts on this subject based on the ideXlab platform.

  • Club Cell secretory protein serum concentration is a surrogate marker of small airway involvement in asthmatic patients
    The Journal of Allergy and Clinical Immunology, 2017
    Co-Authors: Sebastien Bommart, Anne Sophie Gamez, Aurelie Petit, Lucie Knabe, Nicolas Molinari, Isabelle Vachier, Pascal Chanez, Gregory Marin, Catherine Devautour, Arnaud Bourdin
    Abstract:

    Poor asthma control and recurrent exacerbations have been shown to be a phenotypic counterpart of asthma with predominantly small-airway involvement.1 Biomarkers are not always accurate in asthmatic patients, especially in serum, because compartmentalization can occur between the blood and airways. Blood eosinophil counts do not represent an overall view of airway inflammation, and exhaled nitric oxide measurements at different flow rates (fraction of exhaled nitric oxide [Feno] and alveolar nitric oxide [Calvno]) have been developed and validated to reflect more accurately proximal and distal airway inflammation.2 Club Cell secretory protein (CCSP) serum concentration has been shown to be associated with chronic obstructive pulmonary disease, bronchiolitis obliterans syndrome, and sarcoidosis, which are all predominantly diseases involving the small airways. Ranges of CCSP concentrations in healthy subjects, reproducibility, and relationships between serum and airway levels are known and can be used as potential surrogate markers. Our aim was to assess small-airway disease in asthmatic patients and to find a related biomarker. We used a dynamic assessment of gas trapping using computed tomographic (CT) imaging of the chest during methacholine challenge as a marker of small-airway disease.

  • supplementing defect in Club Cell secretory protein attenuates airway inflammation in copd
    Chest, 2015
    Co-Authors: Anne Sophie Gamez, Delphine Gras, Aurelie Petit, Lucie Knabe, Nicolas Molinari, Isabelle Vachier, Pascal Chanez, Arnaud Bourdin
    Abstract:

    BACKGROUND Club Cell secretory protein (CCSP) is a protective biomarker associated with annual decline in lung function. COPD progression results from an imbalance between injury and repair initially triggered by cigarette smoking. OBJECTIVE We investigated the effect of CCSP as a therapeutic strategy to restore the balance between injury and repair in COPD simultaneously, validating an ex vivo air-liquid interface (ALI) culture of human bronchial epithelial Cells. METHODS Endobronchial biopsy specimens (EBBs) were obtained from 13 patients with COPD, eight smokers, and eight control subjects. Morphometric analysis of the initial EBBs was performed. ALI cultures derived from the same EBBs were exposed to cigarette smoke extract (CSE) with or without exogenous recombinant human CCSP (rhCCSP) supplementation. CCSP and IL-8 concentrations were assessed at steady state and after CSE exposure. RESULTS Morphometric analysis of the initial EBBs showed increased Cell density but decreased immunostaining of CCSP+ Cells in EBBs of patients with COPD (P = .03 vs control subjects). At steady state, lower CCSP (P = .04) and higher IL-8 levels (P CONCLUSIONS In vitro, rhCCSP exogenous supplementation can reverse CSE-induced IL-8 release in biopsy specimens from patients with COPD, indicating a potential use of this strategy in vivo.

  • Club Cells, CC10 and self-control at the epithelial surface
    European Respiratory Journal, 2014
    Co-Authors: Pieter S. Hiemstra, Arnaud Bourdin
    Abstract:

    There has been a recent marked increase in our insight into mechanisms that control inflammation, unwanted immune responses and tissue injury. This is illustrated by the wealth of literature on the role of regulatory T- and B-Cells in lung disease, but also by studies on anti-inflammatory cytokines such as interleukin (IL)-10, and products of microbial metabolism such as short-chain fatty acids. The airway epithelium also shows clear signs of self-control, and studies on the Club Cell 10-kDa protein (CC10; also known as e.g. Club Cell secretory protein or secretoglobin family 1A member 1 (SCGB1A1)) have identified it as one of the mediators involved [1]. CC10 is a main product of Club Cells, which are especially abundant in the peripheral airways where they contribute to maintenance of airway integrity and repair [2]. The consequences of injury to the small airways are clear from the involvement of small airway dysfunction in an increasing number of lung diseases. Moreover, a defect in the relative abundance of CC10-expressing Cells in the peripheral airways of patients with asthma, chronic obstructive pulmonary disease (COPD) and post-transplant obliterative bronchiolitis was reported …

Douglas P Chivers - One of the best experts on this subject based on the ideXlab platform.

  • the interactive effects of multiple stressors on physiological stress responses and Club Cell investment in fathead minnows
    Science of The Total Environment, 2014
    Co-Authors: Aditya K Manek, Maud C O Ferrari, Som Niyogi, Douglas P Chivers
    Abstract:

    Abstract Anthropogenic activities have dramatically increased over the past decades, with the consequence that many organisms are simultaneously exposed to multiple stressors. Understanding how organisms respond to these stressors is a key focus for scientists from many disciplines. Here we investigated the interactive effects of two stressors, UV radiation (UVR) and cadmium (Cd) exposure on a common freshwater fish, fathead minnow ( Pimephales promelas ). UVR is known to influence the density of epidermal Club Cells (ECCs), which are not only a key component of the innate immune system of fishes, but are also the source of chemical alarm cues that serve to warn other fishes of nearby predators. In contrast, Cd impairs the physiological stress response and ability of fish to respond to alarm cues. We used an integrative approach to examine physiological stress response as well as investment in ECCs. Fish exposed to UVR had higher levels of cortisol than non-exposed controls, but Cd reduced cortisol levels substantially for fish exposed to UVR. Fish exposed to UVR, either in the presence or absence of Cd, showed consistent decreases in ECC investment compared to non-exposed controls. Despite differences in ECC number, there was no difference in the potency of alarm cues prepared from the skin of UVR and Cd exposed or non-exposed fish indicating that UVR and Cd exposure combined may have little influence on chemically-mediated predator–prey interactions.

  • within and between population variation in epidermal Club Cell investment in a freshwater prey fish a cautionary tale for evolutionary ecologists
    PLOS ONE, 2013
    Co-Authors: Aditya K Manek, R J Pollock, Maud C O Ferrari, Daniel Vicente, Lynn P Weber, Douglas P Chivers
    Abstract:

    Many prey fishes possess large Club Cells in their epidermis. The role of these Cells has garnered considerable attention from evolutionary ecologists. These Cells likely form part of the innate immune system of fishes, however, they also have an alarm function, releasing chemical cues that serve to warn nearby conspecifics of danger. Experiments aimed at understanding the selection pressures leading to the evolution of these Cells have been hampered by a surprisingly large intraspecific variation in epidermal Club Cell (ECC) investment. The goal of our current work was to explore the magnitude and nature of this variation in ECC investment. In a field survey, we documented large differences in ECC investment both within and between several populations of minnows. We then tested whether we could experimentally reduce variation in mean ECC number by raising fish under standard laboratory conditions for 4 weeks. Fish from different populations responded very differently to being held under standard laboratory conditions; some populations showed an increase in ECC investment while others remained unchanged. More importantly, we found some evidence that we could reduce within population variation in ECC investment through time, but could not reduce among-population variation in mean ECC investment. Given the large variation we observed in wild fish and our limited ability to converge mean Cell number by holding the fish under standard conditions, we caution that future studies may be hard pressed to find subtle effects of various experimental manipulations; this will make elucidating the selection pressures leading to the evolution of the Cells challenging.

  • the effects of ultraviolet radiation on a freshwater prey fish physiological stress response Club Cell investment and alarm cue production
    Biological Journal of The Linnean Society, 2012
    Co-Authors: Aditya K Manek, Maud C O Ferrari, Som Niyogi, Jeff M Sereda, Douglas P Chivers
    Abstract:

    Recent anthropogenic activities have caused deleterious effects to the stratospheric ozone layer, resulting in a global increase in the level of ultraviolet radiation (UVR). Understanding the way that organisms respond to such stressors is key to predicting the effects of anthropogenic activities on aquatic ecosystems and the species that inhabit them. The epidermal layer of the skin of fishes is not keratinized and acts as the primary interface between the fish and its environment. The skin of many species of fishes contains large epidermal Club Cells (ECCs) that are known to release chemicals (alarm cues) serving to warn other fishes of danger. However, the alarm role of the Cells is likely secondary to their role in the immune system. Recent research suggests that ECCs in the epidermis may play a role in protecting the fish from damage caused by UVR. In the present study, we examined the effects of in vivo exposure to UVR on fathead minnows (Pimephales promelas), specifically investigating ECC investment, physiological stress responses, and alarm cue production. We found that fish exposed to UVR showed an increase in cortisol levels and a substantive decrease in ECC investment compared to non-exposed controls. Unexpectedly, our subsequent analysis of the behavioural response of fish to alarm cues revealed no difference in the potency of the cues prepared from the skin of UV-exposed or non-exposed minnows. Our results indicate that, although nonlethal, UVR exposure may lead to secondary mortality by altering the fish immune system, although this same exposure may have little influence on chemically-mediated predator–prey interactions. © 2012 The Linnean Society of London, Biological Journal of the Linnean Society, 2012, 105, 832–841.

  • do fathead minnows pimephales promelas rafinesque alter their Club Cell investment in responses to variable risk of infection from saprolegnia
    Journal of Fish Diseases, 2012
    Co-Authors: R J Pollock, Michael S Pollock, Maud C O Ferrari, Susan G W Kaminskyj, Douglas P Chivers
    Abstract:

    Fish in the Superorder Ostariophysi possess large epidermal Club Cells that release chemical cues warning nearby conspecifics of danger. Despite the long‐held assumption that such Club Cells evolved under the selective force of predation, recent studies demonstrated that predation has no effect on Club Cell investment. Rather, Club Cells have an immune function and Cell production may be stimulated by skin‐penetrating pathogens and parasites. The current work investigates whether fathead minnows, Pimephales promelas, alter their Club Cell characteristics based on variation in infection risk. In a 2 A— 3 design, we exposed minnows to infective cysts of two oomycete species (Saprolegnia ferax and S. parasitica) at three different concentrations (2, 20 or 200 cysts L−1). Club Cell characteristics (number and size) were quantified 12 days after exposure. Saprolegnia parasitica is thought to be more pathogenic than S. ferax, hence we predicted greater Club Cell investment and a larger turnover rate of Cells by minnows exposed to S. parasitica than S. ferax. We also predicted that minnows exposed to higher numbers of cysts should invest more in Club Cells and have a higher turnover rate of Cells. We found no difference in Club Cell density or size between fish exposed to the two Saprolegnia species; however, fish exposed to high concentrations of pathogens had smaller Club Cells than those exposed to low concentrations, indicating a higher rate of turnover of Cells in the epidermis.

Alfred Bernard - One of the best experts on this subject based on the ideXlab platform.

  • urinary Club Cell protein 16 cc16 utility of its assay during acute bronchiolitis
    Pediatric Pulmonology, 2020
    Co-Authors: C Egron, A Labbe, Emmanuelle Rochette, Aurelien Mulliez, Alfred Bernard, Amat Flore
    Abstract:

    : Acute bronchiolitis is responsible for high morbidity in infants. Club Cell protein 16 kDa (CC16) is a major pneumoprotein secreted by Club Cells of the bronchial epithelium and eliminated by the renal pathway. CC16 seems to be a biomarker of epithelial damage in asthma. However, its value as a marker of acute bronchiolitis severity and later recurrent wheezing are uncertain, especially the value of its urinary assay for this purpose. A prospective, observational, analytical study was conducted at Clermont-Ferrand University Hospital to correlate serum CC16 level with clinical severity of bronchiolitis in hospitalized infants aged less than 1 year. We analyzed correlations between serum and urinary CC16, CC16 levels and Wainwright score, immediate morbidity due to bronchiolitis, causal viruses, and recurrent wheezing 1 year after inclusion. In 166 infants, serum CC16 did not correlate with acute bronchiolitis severity (P = .49), but urinary CC16 did (P < .001). In multivariate analysis, urinary CC16 correlated mainly with urinary retinol binding protein (RBP; r = 0.70; P < .001). The logCC16u/logRBPu ratio correlated significantly with severity (P = .02). CC16 levels were not correlated with recurrent wheezing at 1 year. Urinary CC16 could be a useful biomarker in acute bronchiolitis for specific indications. This noninvasive assay would be particularly useful in the young infant population. Several factors must be taken into account in its interpretation, mainly tubular function. Further studies are needed to assess these factors.

  • serum Club Cell protein 16 is associated with asymptomatic airway responsiveness in adults findings from the french epidemiological study on the genetics and environment of asthma
    Respirology, 2015
    Co-Authors: Marta Rava, Alfred Bernard, Stefano Guerra, Nicole Le Moual, Xavier Dumont, Valerie Siroux, Benedicte Jacquemin, Francine Kauffmann, Rachel Nadif
    Abstract:

    Club Cell secretory protein (CC-16) is a sensitive biomarker of airways epithelium integrity. It has gained interest as a biological marker in chronic lung diseases because of its presumed relationship to inflammation. Little is known about the association between CC-16 serum level and asthma, lung function and airway responsiveness (AR). Serum CC-16 level was determined by latex immunoassay in 1298 participants from the French Epidemiological case-control and family-based study on Genetics and Environment of Asthma (EGEA) (mean age 43 years; 49% men, 38% with asthma). Pre-bronchodilator lung function (forced expiratory volume in 1 s (FEV1 ), forced vital capacity (FVC) and FEV1 /FVC) and degree of AR, expressed as a function of the dose-response slope to methacholine test were measured. Standardized residuals CC-16 z-scores were obtained by regressing CC-16 level on the glomerular filtration rate. CC-16 z-scores were correlated with asthma, lung function and AR in participants with and without asthma. CC-16 geometric mean level was 12.4 μg/L (range: 2.2-70.6 μg/L). In participants without asthma, lower CC-16 z-scores was associated with impaired FEV1 /FVC% (β = 0.50 (95% CI: 0.06, 0.95) and with higher degree of AR (β = 0.24 (95% CI: 0.09, 0.39)). CC-16 was not associated with impaired lung function or AR in participants with asthma. Lower CC-16 serum level was associated with impaired lung function and AR, suggesting that serum CC-16 level may reflect early damages to the lung epithelium in adults without asthma.

  • serum levels of Club Cell secretory protein clara and short and long term exposure to particulate air pollution in adolescents
    Environment International, 2014
    Co-Authors: Alfred Bernard, Eline B Provost, Agnes Chaumont, Michal Kicinski, Frans Fierens, Tim S Nawrot
    Abstract:

    Belgian science policy under the Science for Sustainable Development program (grant number SD/HE/01); Flemish Scientific fund (grant number 1.2.506.07.N.00/G.0.919.10.N.10); EU program 'ENVIRONAGE' (grant number ERC-2012-StG310898); Ph. D. fellowship of the Research Foundation-Flanders (FWO); VITO-FWO Ph. D. fellowship