The Experts below are selected from a list of 321 Experts worldwide ranked by ideXlab platform
Jian Chen - One of the best experts on this subject based on the ideXlab platform.
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Acrylate copolymer: a rate-Controlling Membrane in the transdermal drug delivery system
e-Polymers, 2015Co-Authors: Xiaoping Zhan, Zhenmin Mao, Jian Chen, Yuankui ZhangAbstract:AbstractA film-like copolymer composed of 2-hydroxy-3-phenoxypropylacrylate, 4-hydroxybutyl acrylate and cyclohexyl methacrylate was synthesized and exploited as a rate-Controlling Membrane in the transdermal drug delivery systems (TDDs). A series of acrylate copolymers with different formulations were characterized by Fourier transform infrared spectroscopy, differential scanning calorimetry and tensile strength, and then evaluated by clonidine hydrochloride transporting through the films. It was found that the formulation M2 composed of three monomers at a ratio of 4:4:2 (w/w/w) showed excellent mechanical and permeation properties. The optimal formulation M2 was further characterized by scanning electron microscopy, contact angles and swelling ratio, and then the permeation behaviors of five different physicochemical drugs transporting through the M2 were evaluated. The results showed that the permeation behaviors were influenced by many factors including the thickness of the Membrane, the physicochemical properties of the drugs, the dose of the drugs and the interactions between the drugs and the Membrane. This type of copolymer Membrane might open new applications in the field of TDDs.
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treatment of synthetic wastewater by a novel mbr with granular sludge developed for Controlling Membrane fouling
Separation and Purification Technology, 2005Co-Authors: Fangshu Gao, Zhaozhe Hua, Jian ChenAbstract:Abstract A novel Membrane bioreactor system, a Membrane bioreactor with aerobic granular sludge (MGSBR), was developed to treat municipal wastewater in this paper. The main goal was to control the Membrane fouling by changing the configuration of aerobic sludge, also to examine the feasibility of the MGSBR used to treat wastewater. The results in the paper demonstrated that treatment performance of a MGSBR was stable and on a high level. The ranges of COD degradation and concentration in the effluent from a MGSBR were 80–95% and 15–50 mg L −1 , respectively, during the period of operation examined. Compared with the conventional MBR (MFSBR) system, anaerobic environment in a MGSBR system could be effectively provided inside aerobic granules, which benefited for the denitrification of nitrate nitrogen. The NH 4 –N concentration in effluent from the MGSBR system was all the time lower than 15 mg L −1 . During the examined time, the Membrane permeability of MGSBR was more 50% higher than that of a MFSBR. The substitute of floc sludge by aerobic granules in the MBR system benefited for Controlling flux decline and Membrane fouling with good performance of treatment. The supernatant (colloids + solutes) was almost the controller of the Membrane flux in a MGSBR. The cleaning recovery of hydraulic cleaning in Membrane flux from the MGSBR was 87.7%, which was near 10% lower than that of MFSBR system. However, the chemical cleaning could remove most of foulants on the Membrane surface and within the pores with the recovery of near 100%. As a novel MBR system possessing advantages in Controlling of Membrane fouling with good performance of wastewater treatment, the MGSBR process deserved more attention and deep investigation in the future.
Henry R. Bourne - One of the best experts on this subject based on the ideXlab platform.
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Localization of a peripheral Membrane protein: Gβγ targets GαZ
Proceedings of the National Academy of Sciences of the United States of America, 2000Co-Authors: C. Simone Fishburn, Sonia K. Pollitt, Henry R. BourneAbstract:To explore the relative roles of protein-binding partners vs. lipid modifications in Controlling Membrane targeting of a typical peripheral Membrane protein, Gαz, we directed its binding partner, βγ, to mislocalize on mitochondria. Mislocalized βγ directed wild-type Gαz and a palmitate-lacking Gαz mutant to mitochondria but did not alter localization of a Gαz mutant lacking both myristate and palmitate. Thus, in this paradigm, a protein–protein interaction controls targeting of a peripheral Membrane protein to the proper compartment, whereas lipid modifications stabilize interactions of proteins with Membranes and with other proteins.
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Localization of a peripheral Membrane protein: Gβγ targets GαZ
Proceedings of the National Academy of Sciences of the United States of America, 2000Co-Authors: C. Simone Fishburn, Sonia K. Pollitt, Henry R. BourneAbstract:To explore the relative roles of protein-binding partners vs. lipid modifications in Controlling Membrane targeting of a typical peripheral Membrane protein, Galpha(z), we directed its binding partner, betagamma, to mislocalize on mitochondria. Mislocalized betagamma directed wild-type Galpha(z) and a palmitate-lacking Galpha(z) mutant to mitochondria but did not alter localization of a Galpha(z) mutant lacking both myristate and palmitate. Thus, in this paradigm, a protein-protein interaction controls targeting of a peripheral Membrane protein to the proper compartment, whereas lipid modifications stabilize interactions of proteins with Membranes and with other proteins.
Sangmyung Rhee - One of the best experts on this subject based on the ideXlab platform.
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mdia1 regulates breast cancer invasion by Controlling Membrane type 1 matrix metalloproteinase localization
Oncotarget, 2016Co-Authors: Daehwan Kim, Jangho Jung, Eunae You, Sangmyung RheeAbstract:// Daehwan Kim 1 , Jangho Jung 1 , Eunae You 1 , Panseon Ko 1 , Somi Oh 1 , Sangmyung Rhee 1 1 Department of Life Science, Chung-Ang University, Seoul 06974, Korea Correspondence to: Sangmyung Rhee, e-mail: Sangmyung.rhee@cau.ac.kr Keywords: breast cancer cells, invasion, mDia1, Membrane type 1-matrix metalloproteinase (MT1-MMP), microtubule dynamics Received: July 27, 2015 Accepted: February 11, 2016 Published: February 17, 2016 ABSTRACT Mammalian diaphanous-related formin 1 (mDia1) expression has been linked with progression of malignant cancers in various tissues. However, the precise molecular mechanism underlying mDia1-mediated invasion in cancer cells has not been fully elucidated. In this study, we found that mDia1 is upregulated in invasive breast cancer cells. Knockdown of mDia1 in invasive breast cancer profoundly reduced invasive activity by Controlling cellular localization of Membrane type 1-matrix metalloproteinase (MT1-MMP) through interaction with microtubule tracks. Gene silencing and ectopic expression of the active form of mDia1 showed that mDia1 plays a key role in the intracellular trafficking of MT1-MMP to the plasma Membrane through microtubules. We also demonstrated that highly invasive breast cancer cells possessed invasive activity in a 3D culture system, which was significantly reduced upon silencing mDia1 or MT1-MMP. Furthermore, mDia1-deficient cells cultured in 3D matrix showed impaired expression of the cancer stem cell marker genes, CD44 and CD133. Collectively, our findings suggest that regulation of cellular trafficking and microtubule-mediated localization of MT1-MMP by mDia1 is likely important in breast cancer invasion through the expression of cancer stem cell genes.
Feng Zhang - One of the best experts on this subject based on the ideXlab platform.
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Membrane fouling control in a submerged Membrane bioreactor with porous flexible suspended carriers
Desalination, 2006Co-Authors: Qiyong Yang, Jihua Chen, Feng ZhangAbstract:Abstract Membrane fouling has been the main obstacle to the wide application of Membrane bioreactors (MBR). A hybrid Membrane bioreactor (HMBR) with porous, flexible suspended carriers was explored that was efficient in Controlling Membrane fouling, especially cake layers. In order to investigate the influence of suspended carriers on the filtration performance of the HMBR, two kinds of bioreactors—MBR and HMBR—were tested in parallel processing. In the short-term trials, the critical flux of HMBR increased by 20% and the cake resistance of HMBR decreased by 86% in comparison with MBR. During the long-term experiments, the increase rate of suction pressure for HMBR accounted for 30% of that for MBR, indicating that the degree of Membrane fouling for HMBR was far lower than that for MBR. These observations revealed that suspended carriers play an important role in governing the filtration conditions and decreasing fouling resistance. In order to understand the influence of suspended carriers better on the system performance, several parameters such as cake layers of Membrane surface, characteristics of the mixed liquor and filterability of the supernatant and the activated sludge suspension were investigated.
C. Simone Fishburn - One of the best experts on this subject based on the ideXlab platform.
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Localization of a peripheral Membrane protein: Gβγ targets GαZ
Proceedings of the National Academy of Sciences of the United States of America, 2000Co-Authors: C. Simone Fishburn, Sonia K. Pollitt, Henry R. BourneAbstract:To explore the relative roles of protein-binding partners vs. lipid modifications in Controlling Membrane targeting of a typical peripheral Membrane protein, Gαz, we directed its binding partner, βγ, to mislocalize on mitochondria. Mislocalized βγ directed wild-type Gαz and a palmitate-lacking Gαz mutant to mitochondria but did not alter localization of a Gαz mutant lacking both myristate and palmitate. Thus, in this paradigm, a protein–protein interaction controls targeting of a peripheral Membrane protein to the proper compartment, whereas lipid modifications stabilize interactions of proteins with Membranes and with other proteins.
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Localization of a peripheral Membrane protein: Gβγ targets GαZ
Proceedings of the National Academy of Sciences of the United States of America, 2000Co-Authors: C. Simone Fishburn, Sonia K. Pollitt, Henry R. BourneAbstract:To explore the relative roles of protein-binding partners vs. lipid modifications in Controlling Membrane targeting of a typical peripheral Membrane protein, Galpha(z), we directed its binding partner, betagamma, to mislocalize on mitochondria. Mislocalized betagamma directed wild-type Galpha(z) and a palmitate-lacking Galpha(z) mutant to mitochondria but did not alter localization of a Galpha(z) mutant lacking both myristate and palmitate. Thus, in this paradigm, a protein-protein interaction controls targeting of a peripheral Membrane protein to the proper compartment, whereas lipid modifications stabilize interactions of proteins with Membranes and with other proteins.