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Eder C. Lima - One of the best experts on this subject based on the ideXlab platform.

  • magnetic activated carbon nanocomposite from nigella sativa l waste mnsa for the removal of Coomassie Brilliant Blue dye from aqueous solution statistical design of experiments for optimization of the adsorption conditions
    Journal of Advanced Research, 2019
    Co-Authors: N T Abdelghani, El-shaimaa A. Rawash, Ghadir A Elchaghaby, Eder C. Lima
    Abstract:

    Abstract The present work was carried out to evaluate the removal of Coomassie Brilliant Blue dye by adsorption onto a magnetized activated carbon nanocomposite (MNSA) prepared from Nigella sativa L. (NS) waste. Different techniques, including infrared spectroscopy, scanning electron microscopy, and nitrogen adsorption/desorption, were used to characterize MNSA to investigate its adsorption properties. Adsorption experiments were carried out by simultaneously optimizing four variables that usually present a strong effect in adsorption studies. A full 24 factorial design with 3 central points was used. The four independent variables were the initial pH of the dye solution (pH), the initial dye concentration (Co), the adsorbent mass (m), and the contact time (t). The sorption capacity (q) of the adsorbent and the percentage of dye removal (% Rem) from an aqueous solution were used as the responses of the factorial design. The results indicated that pH, Co, and m were essential factors for the overall optimization of both responses (q and % Rem) and that several interactions of two, three and four factors occurred. Based on the design of the experiments (DOE), the optimized conditions for adsorption were pH = 2.00, Co = 40.0 mg L−1, m = 30.0 mg, and t = 3.0 h. Under these conditions, both responses, q and % Rem, were maximized, with a desirability of 85.54%. The findings of this study could be useful for industrial wastewater treatment systems.

  • Magnetic activated carbon nanocomposite from Nigella sativa L. waste (MNSA) for the removal of Coomassie Brilliant Blue dye from aqueous solution: Statistical design of experiments for optimization of the adsorption conditions
    'Elsevier BV', 2019
    Co-Authors: Nour T. Abdel-ghani, Ghadir A. El-chaghaby, El-shaimaa A. Rawash, Eder C. Lima
    Abstract:

    The present work was carried out to evaluate the removal of Coomassie Brilliant Blue dye by adsorption onto a magnetized activated carbon nanocomposite (MNSA) prepared from Nigella sativa L. (NS) waste. Different techniques, including infrared spectroscopy, scanning electron microscopy, and nitrogen adsorption/desorption, were used to characterize MNSA to investigate its adsorption properties. Adsorption experiments were carried out by simultaneously optimizing four variables that usually present a strong effect in adsorption studies. A full 24 factorial design with 3 central points was used. The four independent variables were the initial pH of the dye solution (pH), the initial dye concentration (Co), the adsorbent mass (m), and the contact time (t). The sorption capacity (q) of the adsorbent and the percentage of dye removal (% Rem) from an aqueous solution were used as the responses of the factorial design. The results indicated that pH, Co, and m were essential factors for the overall optimization of both responses (q and % Rem) and that several interactions of two, three and four factors occurred. Based on the design of the experiments (DOE), the optimized conditions for adsorption were pH = 2.00, Co = 40.0 mg L−1, m = 30.0 mg, and t = 3.0 h. Under these conditions, both responses, q and % Rem, were maximized, with a desirability of 85.54%. The findings of this study could be useful for industrial wastewater treatment systems. Keywords: Nigella sativa L. waste, Nanocomposite, Coomassie Brilliant Blue, Central composite design, Adsorptio

  • adsorption of Coomassie Brilliant Blue r 250 dye onto novel activated carbon prepared from nigella sativa l waste equilibrium kinetics and thermodynamics running title adsorption of Brilliant Blue dye onto nigella sativa l waste activated carbon
    Journal of The Chilean Chemical Society, 2017
    Co-Authors: N T Abdelghani, El-shaimaa A. Rawash, Ghadir A Elchaghaby, Eder C. Lima
    Abstract:

    In this paper a novel adsorbent was prepared from Nigella sativa L. waste and used for the removal of Coomassie Brilliant Blue dye from wastewater. The preparation of Nigella sativa waste carbon (NSWC) was achieved by adding concentrated sulfuric acid to the precursor material at an impregnation ratio (1: 1) and the mixture was left overnight. The resulting material was washed with sodium bicarbonates and finally oven dried. The kinetics of Coomassie Brilliant Blue adsorption onto NSWC was investigated by three kinetic models. The pseudo-second order model (R2 = 0.99) was the best model fitted the experimental data.The equilibrium results revealed that Freundlich model was the best isotherm model fitted (R2= 0.994). Also the value of the Freundlich exponent (n) was found to be 1.174 suggesting the favorable dye adsorption onto NSWC. The thermodynamics results indicated negative values of ΔG proving the spontaneous nature of Brilliant Blue dye adsorption on NSWC. The exothermic nature of the adsorption was also confirmed by the negative value of change in enthalpy ΔH°. Also, the negative value of the activation entropy ΔS° demonstrates the decreased randomness at the solid–solution interface during adsorption. The present study results suggest the possible use of a waste such as Nigella sativa L. waste as a precursor for the development of a new cheap and efficient adsorbent that could be used in dyes removal from wastewater.

Biji T Kurien - One of the best experts on this subject based on the ideXlab platform.

  • destaining Coomassie Brilliant Blue stained sodium dodecyl sulfate polyacrylamide protein gels using a household detergent
    Methods of Molecular Biology, 2018
    Co-Authors: Rachna Aggarwal, Biji T Kurien
    Abstract:

    We report here a one-step method using the household detergent Vim Ultra to destain sodium dodecyl sulfate-polyacrylamide protein gels stained with Coomassie Brilliant Blue. This method, originally described by Pal and group, uses a 5% suspension of the detergent to destain gels efficiently. This method is cheap and user-friendly compared to the commonly used methanol-acetic acid-water containing destaining solvent.

  • Coomassie Brilliant Blue removal disposal from gel destain and used gel stain in an environment friendly manner
    Methods of Molecular Biology, 2012
    Co-Authors: Biji T Kurien, Yaser Dorri
    Abstract:

    : Toxic reagents are employed to destain Coomassie Brilliant Blue (CBB) stained gels. We tested the efficacy of various paper adsorbents in adsorbing CBB released from gels during destaining. Kimwipes were the most efficient, followed by Teri towels, multifold towels, and Whatman (numbers 1 and 3) filter papers. Three Kimwipes added during destaining of a CBB-stained mini-gel helped adsorb the released dye. Thus, stain removal with Kimwipes helps reduce destain use and organic waste accumulation, enables recycling of nonradioactive destaining solution, and is 7.5-fold cheaper than an available method for CBB disposal. Next, we used Kimwipes to deplete the dye from a used CBB staining solution awaiting proper disposal by our Institutional Safety Office. Seventy-five Kimwipes successfully helped remove the dye from a 0.05% CBB staining solution in 5 to 10 min. The Blue-colored Kimwipes did not release the stain even when squeezed dry after incubation in various salts, water, or acid solutions for five weeks. The CBB removed thus can be simply disposed as solid waste and will not leach out from solid landfills. Kimwipes, thus, enables CBB disposal in an environmentally friendly manner and allows recycling of destaining solution.

  • environmentally safe removal disposal of Coomassie Brilliant Blue from gel destain and used gel stain
    Analytical Biochemistry, 2010
    Co-Authors: Yaser Dorri, Biji T Kurien
    Abstract:

    Gel destaining following Coomassie Brilliant Blue (CBB) staining involves the use of toxic reagents. Here we demonstrate the efficacy of various paper adsorbents in adsorbing CBB. Kimwipes adsorbed the best, followed by Teri towels, multifold towels, and Whatman numbers 1 and 3 filter papers. Three Kimwipes completely adsorbed the dye released from a CBB-stained mini-gel. Nonradioactive destain solution can, therefore, be recycled for destaining CBB-stained gels. Stain removal with Kimwipes helps in reducing destain use and in reducing organic liquid waste, and it is 7.5-fold cheaper compared with an available method for CBB disposal. Following this, we determined the suitability of this procedure to remove the dye from a used CBB staining solution awaiting proper disposal by our Institutional Safety Office. The dye from a 0.05% CBB staining solution could be removed in 5 to 10 min using 75 Kimwipes. The CBB-adsorbed Kimwipes did not release the stain when squeezed dry even after incubation in various salts over 1 week and in water for 5 weeks. The CBB removed allows its easy disposal as solid waste and will not leach out from solid landfills. Thus, stain removal with Kimwipes helps in disposing CBB in an environmentally friendly manner and allows recycling of destaining solution.

Katherine M Williams - One of the best experts on this subject based on the ideXlab platform.

  • interference in the Coomassie Brilliant Blue and pyrogallol red protein dye binding assays is increased by the addition of sodium dodecyl sulfate to the dye reagents
    Analytical Biochemistry, 2004
    Co-Authors: Thomas Marshall, Katherine M Williams
    Abstract:

    Abstract We have investigated the effect of sodium dodecyl sulfate (SDS) upon the response of the Coomassie Brilliant Blue (CBB) and Pyrogallol Red-molybdate (PRM) protein dye-binding assays to interference from aminoglycosides, ampholytes, detergents, phenothiazines, reducing agents, and miscellaneous substances previously reported to interfere with the assays. The CBB assay was less prone to interference than the PRM assay but gave positive interference with the detergents and the phenothiazines and negative interference with dextran sulfate. The PRM assay gave positive interference with the aminoglycosides, ampholytes, and phenothiazines and negative interference with SDS, citric acid, dextran sulfate, EDTA, oxalic acid, and tartaric acid. The level of interference varied in the presence of different proteins (albumin, gamma globulin, α 1 -acid glycoprotein, or lysozyme) and increased when SDS was added to the dye reagents.

  • recovery of protein by Coomassie Brilliant Blue precipitation prior to electrophoresis
    Electrophoresis, 1992
    Co-Authors: Thomas Marshall, Katherine M Williams
    Abstract:

    The interaction of protein with Coomassie Brilliant Blue G-250 results in formation of an insoluble protein-dye complex which can be recovered by centrifugation and redissolved for electrophoretic analysis. The precipitated protein can be washed in acetone to remove excess dye in order to enhance resolution. The residual dye becomes dissociated from the proteins on electrophoresis and can be exploited as a "dye front". The method allows simultaneous protein assay and recovery of microgram amounts of protein from dilute solution and could be widely applied for conserving, concentrating and desalting minute amounts of valuable sample prior to electrophoretic analysis.

Chunyi Liau - One of the best experts on this subject based on the ideXlab platform.

Gyurngsoo Yoo - One of the best experts on this subject based on the ideXlab platform.