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Guisla Boehs - One of the best experts on this subject based on the ideXlab platform.

  • micronutrientes metalicos nos moluscos bivalves Crassostrea rhizophorae e mytella guyanensis
    Acta Brasiliensis, 2021
    Co-Authors: Glaucia Batista Maciel Dos Santos, Guisla Boehs
    Abstract:

    Micronutrients, micro or oligo elements, include vitamins and some metals, being that a significant portion of the world population is nutritionally deficient in these elements. This study evaluated the levels of metallic micronutrients in Crassostrea rhizophorae (oyster) and Mytella guyanensis (mangrove mussel) collected in southern Bahia, Brazil, in a stretch of 280 km long. The metal contents (Cu, Fe, Mn and Zn) of the edible part of the species were evaluated with inductively coupled plasma optical emission spectrometry (ICP OES). Mean concentrations in mg kg-1 found for C. rhizophorae and M. guyanensis, respectively, were: Cu (2.09 ± 0.90 and 2.31 ± 0.63); Fe (32.03 ± 4.18 and 213.36 ± 51.67); Mn (1.75 ± 0.44 and 13.61 ± 2.83) and Zn (129.54 ± 25.88 and 12.13 ± 1.13). Both bivalves were relevant in the concentration of micronutrients investigated, with emphasis on the high values of Zn in C. rhizophorae and Fe in M. guyanensis.

  • microbiological quality and prevalence of β lactam antibiotic resistance genes in oysters Crassostrea rhizophorae
    Journal of Food Protection, 2017
    Co-Authors: Maria Aparecida Da Ressurreicao Brandao, Guisla Boehs, George Rego Albuquerque, Amanda Teixeira Sampaio Lopes, Maria Tereza Da Silva Neta, Rhyan Barros Farias De Oliveira, Rachel Passos Rezende, Veronica Dias Goncalves, Dalia Dos Prazeres Rodrigues, Bianca Mendes Maciel
    Abstract:

    ABSTRACT The microbiological quality of oysters reflects the microbiological quality of their habitats because they are filter feeders. The objective of this study was to assess the bacterial composition of the edible oyster Crassostrea rhizophorae in urban and preserved estuaries. Particularly, we assessed the presence of pathogenic bacteria, investigated antibiotic susceptibility in bacterial isolates, and quantified β-lactam antibiotic resistance genes (blaTEM, blaSHV, and blaKPC) via quantitative PCR of oyster DNA. Our results detected total coliforms, Escherichia coli, and enterobacteria in the oysters from urban estuaries, which is indicative of poor water quality. In addition, our detection of the eaeA and stxA2 virulence genes in 16.7% of E. coli isolates from oysters from this region suggests the presence of multiantibiotic-resistant enteropathogenic and enterohemorrhagic E. coli strains. During periods of low precipitation, increased contamination by E. coli (in winter) and Vibrio parahaemolytic...

  • deteccao de perkinsus marinus na ostra Crassostrea rhizophorae do sul da bahia por analise proteomica
    Brazilian Journal of Veterinary Research and Animal Science, 2017
    Co-Authors: Thiago Ramos Pinto, Guisla Boehs, Mariane Dos Santos Aguiar Luz, Wallace Felipe Blohem Pessoa, Helena Costa
    Abstract:

    Este estudo relata a presenca do patogeno Perkinsus marinus, de notificacao obrigatoria a Organizacao Internacional de Epizootias (OIE) na ostra Crassostrea rhizophorae no sul da Bahia, via analise proteomica. Foram analisadas as ostras Crassostrea brasiliana de um cultivo em espinhel e C. rhizophorae de um manguezal adjacente, na localidade de Porto do Campo, Baia de Camamu, Bahia. As coletas (n = 100) foram efetuadas em outubro de 2012. Em laboratorio, as ostras foram medidas e abertas para a retirada da carne, que foi macerada em gelo seco. Para a extracao das proteinas, foi adotada a adaptacao de um protocolo utilizado para mexilhoes, apos o que foi realizada a separacao na primeira dimensao, por focalizacao isoeletrica (IEF). Os peptideos foram transferidos para um Espectrometro de Massas. Os espectros obtidos foram analisados no software ProteinLynx Global Server 4.2 e tambem pela ferramenta MASCOT (Matrix Science) e comparados com os bancos de dados do SWISSPROT e do NCBI, respectivamente. A identificacao foi evidenciada por meio da beta-tubulina, homologia Perkinsus marinus ATCC 50983 e codigo da proteina no banco de dados NCBI = gi|294889481. Este e o primeiro registro de P. marinus na Bahia e o quarto no Brasil.

  • first record of perkinsus chesapeaki infecting Crassostrea rhizophorae in south america
    Journal of Invertebrate Pathology, 2016
    Co-Authors: Maximiano Pinheiro Dantas Neto, Rachel Costa Sabry, Tereza Cristina Vasconcelos Gesteira, Guisla Boehs, Rubens Galdino Feijo, Jamille Martins Forte, Rodrigo Maggioni
    Abstract:

    Abstract This study investigated Perkinsus spp. infecting Crassostrea rhizophorae from the Jaguaribe River estuary, Ceara, Brazil. Fragments of gills and rectum of the oysters (n = 150) were incubated in Ray’s fluid thioglycollate medium (RFTM). Genus Perkinsus-specific PerkITS85/750 PCR assays were performed and their amplicons were sequenced by the Sanger method. The RFTM assays confirmed Perkinsus spp. The sequencing of the amplified fragments from the rDNA internal transcribed spacers (ITS) of Perkinsus spp. confirmed Perkinsus chesapeaki. Neighbor-Joining analyzes place P. chesapeaki identified in this study in a well-supported clade with other isolates of the same species. This is the first record of P. chesapeaki infecting C. rhizophorae in South America.

  • perkinsus beihaiensis infectando a ostra Crassostrea rhizophorae em cultivo e em estoque natural na baia de camamu bahia brasil
    Brazilian Journal of Veterinary Research and Animal Science, 2016
    Co-Authors: Mariane Dos Santos Aguiar Luz, Guisla Boehs
    Abstract:

    This study investigated the infection of Perkinsus beihaiensis (Perkinsozoa) in the oyster Crassostrea rhizophorae, both from a long-line cultivation system and from a nearby intertidal zone of mangrove, both in the state of Bahia, northeastern Brazil. The collections were performed in October and November 2012, and in January 2013. The oysters (n = 300) were measured, examined macroscopically for signs of infection and then submitted to the following laboratory techniques: histology, Ray's fluid thioglycollate medium assay (RFTM), polymerase chain reaction (PCR) and sequencing, which confirmed the identification of the pathogen. Histological and RFTM analyses showed, respectively, a mean prevalence of 93.3% and of 69%. The infection was usually mild or very mild. There was no significant difference (p > 0.05) between the environments in terms of infection prevalence or severity. This is the first record of P. beihaiensis in the state of Bahia and the second in oysters from Brazil and South America.

César Lodeiros - One of the best experts on this subject based on the ideXlab platform.

  • crecimiento y sobrevivencia de la ostra de mangle Crassostrea rhizophorae bajo condicion de cultivo intermareal y submareal
    Zootecnia Tropical, 2010
    Co-Authors: Maximiano Nunez, César Lodeiros, Enmary Ramirez, Noelis Narvae, Cesar Graziani
    Abstract:

    To evaluate the growth and the survival rates of the oyster Crassostrea rhizophorae in a regime of sub-tidal and inter-tidal culture at Laguna Grande del Obispo, Gulf of Cariaco, Edo. Sucre, Venezuela, seeds of 32-35 mm in back-ventral length of the shell, were placed in baskets maintained in fixed parks. The oysters growth in length and dry biomass as well as mass of the shell and the survival rates in two culture systems were compared in two periods: the first (I), from October 2005 to January 2006, and the second (II) from February to September of 2006. Environmental parameters of temperature, salinity, a chlorophyll, seston, biofouling and rainfall were taken, based on relating them to the variability of the growth and the survival rates. During the first months, for both periods, a high and progressive mortality in the system of subtidal culture was experienced, related to the

  • identification of environmental factors affecting growth and survival of the mangrove oyster Crassostrea rhizophorae guilding 1828 in suspended culture at la restinga lagoon margarita island venezuela
    Zootecnia Tropical, 2009
    Co-Authors: Esperanza Buitrago, Joaquin Buitrago, Luis Freites, César Lodeiros
    Abstract:

    Mangrove oyster, Crassostrea rhizophorae , is one of the mollusks with greatest perspectives for aquaculture development in the Caribbean and the tropical Atlantic coast. Even though, many studies have been carried out to establish its culture, there has not been established which and how environmental factors influence its growth under culture condition. Spats with a mean size of 30 mm were suspended in “Spanish baskets” at a depth of 4 m during 6 months. The experiment was carried on at La Restinga lagoon, Margarita Island, Venezuela. Each month oysters were measured (ventral-dorsal and antero-posterior), and weighted (dry weight from conch, tissue and fouling). Survivorship was also evaluated. Water temperature was recorded continuously and weekly measurements of Secchi depth, salinity, oxygen, total, organic and inorganic seston, and chlorophyll a were determined. Results show 89% increase in ventral-dorsal size reaching sizes of 54.5 mm. Dry weight of tissue at the end of the study period was 0.48 g after a decrease during the warmer months of august and september, indicating temperature as an important factor controlling spawning. The high and constant phytoplanktonic biomass present in La Restinga lagoon showed that food availability was not a limiting factor, which together with the small variability in the studied environmental variables, made impossible to identify factors controlling growth and survival of Crassostrea rhizophorae in long-line suspended culture. The exception was the occasional predation by Cymatium snails.

  • Effects of mass and position of artificial fouling added to the upper valve of the mangrove oyster Crassostrea rhizophorae on its growth and survival
    Aquaculture, 2007
    Co-Authors: César Lodeiros, Esperanza Buitrago, Lee Galindo, John H. Himmelman
    Abstract:

    Abstract We ran an experiment on mangrove oysters Crassostrea rhizophorae to evaluate the effects of adding different masses of artificial fouling to the upper valve, either to the umbo region or the ventral edge of the shell. Growth and survival were quantified after a 30 d period in suspended culture in the La Restinga Lagoon, Venezuela. The artificial fouling was cement weighing 0.5, 1, 2 or 3 fold the mass of the upper valve. No fouling was added to a control group. Fouling mass, but not the position of the artificial fouling, affected growth in shell length. However, only the heaviest fouling (3 times the mass of the upper valve) had a significant effect. In contrast, there was no affect of either fouling mass or position on tissue growth. Finally, our data indicated that mortality could be affected by the position where we added artificial fouling (greater mortality when fouling was added to the ventral edge of the shell), but not by fouling mass. Our study indicates it is unlikely that the levels of natural fouling that develop on oysters in suspended culture would be sufficient to affect either growth or survival.

  • evaluacion del tipo de cestos de cultivo para la ostra de mangle Crassostrea rhizophorae suspendidas en long line y balsa
    Ciencias Marinas, 2006
    Co-Authors: César Lodeiros, Esperanza Buitrago, Alejandro Guerra
    Abstract:

    Resumen es: Se evaluo el crecimiento y la supervivencia de la ostra de mangle Crassostrea rhizophorae (30 mm de longitud maxima) bajo cultivo en dos sistemas de flo...

  • evaluacion del tipo de cestos de cultivo para la ostra de mangle Crassostrea rhizophorae suspendidas en long line y balsa evaluation of culture enclosures for the mangrove oyster Crassostrea rhizophorae suspended from raft and long line systems
    2006
    Co-Authors: César Lodeiros, Esperanza Buitrago, Alejandro Guerra, Isla Margarita
    Abstract:

    We evaluated the growth and survival of the mangrove oyster Crassostrea rhizophorae (30 mm maximum length) reared in two suspended-culture systems (raft and long-line) using three types of enclosures (oyster enclosures, lantern nets and enclosures based on crates used to transport fish), at a density of 750 ind m –2 , in La Restinga Lagoon, Margarita Island, Venezuela, over the course of six months. No significant differences were found in shell growth (length and mass) due to the system and enclosures used; however, raft-cultured oysters showed better tissue growth. At the end of the study, the oysters in lantern nets suspended from rafts obtained a tissue mass that was around 40% higher than that of the rest of the oysters cultured in the other enclosures. Significant differences in oyster survival were not observed for the raft and long-line systems used, but the cumulative survival at the end of the study was significantly higher for the oyster enclosures (52–56%) than for the lantern nets (36–39%) and fish crates (24–25%). We therefore recommend employing rafts and discarding the use of crate-type enclosures in the culture of C. rhizophorae in lagoon systems.

Patricia Mirella Da Silva - One of the best experts on this subject based on the ideXlab platform.

  • Health assessment of the oyster Crassostrea rhizophorae on the southern coast of Bahia, northeastern Brazil
    Colégio Brasileiro de Parasitologia Veterinária, 2013
    Co-Authors: Brandão,rosana Pinho, Patricia Mirella Da Silva
    Abstract:

    This study investigated the health of natural stocks of the oyster Crassostrea rhizophorae on the southern coast of Bahia in northeastern Brazil, during summer and winter 2010, at three localities (sampling points) in the estuaries of the Maraú (Camamu Bay) and Graciosa rivers. A total of 180 oysters (30/sampling point/season) were examined macroscopically for the presence of pathogens and anatomical changes. The specimens were subsequently fixed in Davidson solution, processed for paraffin embedding, sectioned and stained with Harris' hematoxylin and eosin. Histological analysis revealed the presence of Rickettsia-like organisms (RLOs), Ancistrocoma, Trichodina, Sphenophrya, Nematopsis, Urastoma, Bucephalus in the sporocyst phase, a nonspecific metacercaria, and a metacestode of genus Tylocephalum. The prevalence of infection was low except for parasitism by Nematopsis sp. which also caused histopathological changes. The presence of Bucephalus sp. caused parasitic castration. These two pathogens significantly affect the health of C. rhizophorae

  • First report of the protozoan parasite Perkinsus marinus in South America, infecting mangrove oysters Crassostrea rhizophorae from the Paraíba River (NE, Brazil)
    Journal of Invertebrate Pathology, 2013
    Co-Authors: Patricia Mirella Da Silva, Cristhiane Guertler, Liana Pinho Ferreira, Rogério Tubino Vianna, Lucas Nunes Santana, Sergio Fernández-boo, Andrea Ramilo, Antonio Villalba
    Abstract:

    The present work aimed to study the infection by Perkinsus sp. in the mangrove oysters Crassostrea rhizophorae from the estuary of the Paraiba River (Paraiba State, Brazil). Perkinsosis was detected by incubation of oyster gill pieces in Ray's fluid thioglycollate medium. The monthly prevalence values were all above 70%, thus infection was not likely to be a transient event. Perkinsus sp. parasites isolated from eight oysters were propagated in vitro. PCR-RFLP analysis of in vitro cultured cells as well as the sequences of the rDNA ITS region allowed the identification of the in vitro propagated parasites as Perkinsus marinus. Phylogenetic analyses using rDNA ITS region sequences strongly supported the Perkinsus sp. from Paraiba in a monophyletic group with P. marinus. Thus, the results confirmed the species affiliation of Paraiba Perkinsus sp. as P. marinus. This is the first report of P. marinus in Brazil and South America and the first report of P. marinus naturally infecting C. rhizophorae.

  • Perkinsus sp. infecting oyster Crassostrea rhizophorae (Guilding, 1828) on the coast of Bahia, Brazil.
    Journal of invertebrate pathology, 2012
    Co-Authors: Rosana Pinho Brandão, Rachel Costa Sabry, Fernando Ramos Queiroga, Guisla Boehs, Liliane Oliveira Ceuta, Mariane Dos Santos Aguiar Luz, Patricia Mirella Da Silva
    Abstract:

    This study investigated the occurrence of the protozoan Perkinsus in the oyster Crassostrea rhizophorae on the coast of Bahia State, Brazil. The oysters (n = 900) were collected in February-March and July-August 2010. The Ray's fluid thioglycollate medium (RFTM) analysis of gills and rectum revealed hypnospores of Perkinsus sp. with a high mean prevalence (63%). The infection intensity varied from very light to advanced. The polymerase chain reaction confirmed Perkinsus in 87.2% of the RFTM-positive oysters. Histological analysis showed trophozoites and schizonts phagocytized by hemocytes, mainly in the intestine and the stomach epithelium.

  • Parasitological survey of mangrove oyster, Crassostrea rhizophorae, in the Pacoti River Estuary, Ceará State, Brazil.
    Journal of Invertebrate Pathology, 2012
    Co-Authors: Rachel Costa Sabry, Cristhiane Guertler, Liana Pinho Ferreira, Rogério Tubino Vianna, Aime Rachel Magenta Magalhaes, Tereza Cristina Vasconcelos Gesteira, Margherita Anna Barracco, Patricia Mirella Da Silva
    Abstract:

    Abstract The mangrove oyster, Crassostrea rhizophorae (Bivalvia, Ostreidae) is commonly collected by fisherwomen in the estuaries of the Ceara State (CE), Northeastern Brazil. Despite the socioeconomic importance of this natural resource, there are few studies on the health of the oysters in this region. This study aimed to survey pathological changes in the mangrove oyster C. rhizophorae in the estuary of the Pacoti River, CE. Adult oysters were collected in August 2008 (N = 450) and December 2009 (N = 450) at three sites of the Pacoti estuary and in 2010 (N = 600) samplings were done quarterly at one site which has showed the higher prevalence de Perkinsus. Macroscopical and histological analyses were used to evaluate pathological changes, Ray’s Fluid Thioglycollate Medium (RFTM) to detect Perkinsus spp. and polymerase chain reactions (PCR) and DNA sequencing to identify Perkinsus species. In 2009, RFTM assay detected Perkinsus sp. infecting the tissues of C. rhizophorae with low prevalences of 1.3%, 6.7% e 7.3% in sites 1, 2 and 3, respectively, and in 2010, in site 3, prevalence was 2% (12 of 600 oysters). PCR did not confirm any positive case in 2009 and only 5 in 2010. The phylogenetic analyses strongly indicate that the Perkinsus species infecting oysters C. rhizophorae of this study belongs to Perkinsus beihaiensis. The histology confirmed 11 cases of Perkinsus sp. infecting the C. rhizophorae in 2009, and only two cases in 2010. Nematopsis sp. was the protozoan observed with greater prevalence (up 96.7%). Other found protozoa were: Trichodina, Sphenophrya, Ancistrocoma – like and an unknown ovarian parasite. The metazoa found were the polychaete Polydora with high prevalences, a turbellarian, possibly of the genus Urastoma, an unidentified digenean metacercariae and larvae of cestode Tylocephalum. A continuous monitoring of diseases in bivalves from this natural population is recommended, since the phylogenetic analyses indicate the occurrence of P. beihaiensis infecting oysters C. rhizophorae whose pathogenic potential is unknown.

  • First report of Perkinsus sp. infecting mangrove oysters Crassostrea rhizophorae from the Brazilian coast.
    Diseases of Aquatic Organisms, 2009
    Co-Authors: Rachel Costa Sabry, Rafael Diego Rosa, Aime Rachel Magenta Magalhaes, Tereza Cristina Vasconcelos Gesteira, Margherita Anna Barracco, Patricia Mirella Da Silva
    Abstract:

    Protozoan parasites of the genus Perkinsus are considered important pathogens responsible for mass mortalities in several mollusk species worldwide. In the present study we describe for the first time a parasite of the genus Perkinsus infecting the mangrove oyster Crassostrea rhizophorae from the Brazilian coast. Prevalence of this parasite was low in the Pacoti River estuary (Ceara, northeast Brazil) and absent in oysters from southern Brazil. Oyster gill and rectum tissues incubated in Ray's fluid thioglycollate medium (RFTM) revealed the presence of spherical hypnospores (5 to 55 microm diam.). Histological analysis showed the occurrence of typical signet-ring trophozoites and schizonts (3 to 6 microm diam.) infecting connective tissues of several organs and digestive epithelia. PCR assays specific to the genus Perkinsus, followed by cloning and sequencing of the internal transcribed spacer (ITS) region of the ribosomal ribonucleic acid (rRNA) gene complex, confirmed a close phylogenetic relationship between Brazilian Perkinsus sp. and P. beihaiensis infecting Chinese oysters.

Gabriel Soto - One of the best experts on this subject based on the ideXlab platform.

Esperanza Buitrago - One of the best experts on this subject based on the ideXlab platform.

  • identification of environmental factors affecting growth and survival of the mangrove oyster Crassostrea rhizophorae guilding 1828 in suspended culture at la restinga lagoon margarita island venezuela
    Zootecnia Tropical, 2009
    Co-Authors: Esperanza Buitrago, Joaquin Buitrago, Luis Freites, César Lodeiros
    Abstract:

    Mangrove oyster, Crassostrea rhizophorae , is one of the mollusks with greatest perspectives for aquaculture development in the Caribbean and the tropical Atlantic coast. Even though, many studies have been carried out to establish its culture, there has not been established which and how environmental factors influence its growth under culture condition. Spats with a mean size of 30 mm were suspended in “Spanish baskets” at a depth of 4 m during 6 months. The experiment was carried on at La Restinga lagoon, Margarita Island, Venezuela. Each month oysters were measured (ventral-dorsal and antero-posterior), and weighted (dry weight from conch, tissue and fouling). Survivorship was also evaluated. Water temperature was recorded continuously and weekly measurements of Secchi depth, salinity, oxygen, total, organic and inorganic seston, and chlorophyll a were determined. Results show 89% increase in ventral-dorsal size reaching sizes of 54.5 mm. Dry weight of tissue at the end of the study period was 0.48 g after a decrease during the warmer months of august and september, indicating temperature as an important factor controlling spawning. The high and constant phytoplanktonic biomass present in La Restinga lagoon showed that food availability was not a limiting factor, which together with the small variability in the studied environmental variables, made impossible to identify factors controlling growth and survival of Crassostrea rhizophorae in long-line suspended culture. The exception was the occasional predation by Cymatium snails.

  • Effects of mass and position of artificial fouling added to the upper valve of the mangrove oyster Crassostrea rhizophorae on its growth and survival
    Aquaculture, 2007
    Co-Authors: César Lodeiros, Esperanza Buitrago, Lee Galindo, John H. Himmelman
    Abstract:

    Abstract We ran an experiment on mangrove oysters Crassostrea rhizophorae to evaluate the effects of adding different masses of artificial fouling to the upper valve, either to the umbo region or the ventral edge of the shell. Growth and survival were quantified after a 30 d period in suspended culture in the La Restinga Lagoon, Venezuela. The artificial fouling was cement weighing 0.5, 1, 2 or 3 fold the mass of the upper valve. No fouling was added to a control group. Fouling mass, but not the position of the artificial fouling, affected growth in shell length. However, only the heaviest fouling (3 times the mass of the upper valve) had a significant effect. In contrast, there was no affect of either fouling mass or position on tissue growth. Finally, our data indicated that mortality could be affected by the position where we added artificial fouling (greater mortality when fouling was added to the ventral edge of the shell), but not by fouling mass. Our study indicates it is unlikely that the levels of natural fouling that develop on oysters in suspended culture would be sufficient to affect either growth or survival.

  • evaluacion del tipo de cestos de cultivo para la ostra de mangle Crassostrea rhizophorae suspendidas en long line y balsa
    Ciencias Marinas, 2006
    Co-Authors: César Lodeiros, Esperanza Buitrago, Alejandro Guerra
    Abstract:

    Resumen es: Se evaluo el crecimiento y la supervivencia de la ostra de mangle Crassostrea rhizophorae (30 mm de longitud maxima) bajo cultivo en dos sistemas de flo...

  • evaluacion del tipo de cestos de cultivo para la ostra de mangle Crassostrea rhizophorae suspendidas en long line y balsa evaluation of culture enclosures for the mangrove oyster Crassostrea rhizophorae suspended from raft and long line systems
    2006
    Co-Authors: César Lodeiros, Esperanza Buitrago, Alejandro Guerra, Isla Margarita
    Abstract:

    We evaluated the growth and survival of the mangrove oyster Crassostrea rhizophorae (30 mm maximum length) reared in two suspended-culture systems (raft and long-line) using three types of enclosures (oyster enclosures, lantern nets and enclosures based on crates used to transport fish), at a density of 750 ind m –2 , in La Restinga Lagoon, Margarita Island, Venezuela, over the course of six months. No significant differences were found in shell growth (length and mass) due to the system and enclosures used; however, raft-cultured oysters showed better tissue growth. At the end of the study, the oysters in lantern nets suspended from rafts obtained a tissue mass that was around 40% higher than that of the rest of the oysters cultured in the other enclosures. Significant differences in oyster survival were not observed for the raft and long-line systems used, but the cumulative survival at the end of the study was significantly higher for the oyster enclosures (52–56%) than for the lantern nets (36–39%) and fish crates (24–25%). We therefore recommend employing rafts and discarding the use of crate-type enclosures in the culture of C. rhizophorae in lagoon systems.