The Experts below are selected from a list of 309 Experts worldwide ranked by ideXlab platform

M. C. Blázquez - One of the best experts on this subject based on the ideXlab platform.

  • characterization of 10 microsatellite loci in the spiny tailed iguana Ctenosaura hemilopha
    Molecular Ecology Notes, 2006
    Co-Authors: M. C. Blázquez, Ricardo Rodríguez Estrella, Munguia A Vega
    Abstract:

    Ten microsatellite loci were developed for the Baja California spiny-tailed iguana Ctenosaura hemilopha, using an enriched genomic DNA library. In the Cerralvo Island population, seven loci were polymorphic and presented moderate levels of variation. Number of alleles ranged from two to eight (average 4.43), and observed heterozygosity from 0.150 to 0.857 (average 0.492). Polymorphism was detected at six loci on C. hemilopha individuals from the southern Baja California Peninsula. These markers will be useful to study familiar relationships and behaviour on the Cerralvo Island population.

  • Characterization of 10 microsatellite loci in the spiny‐tailed iguana Ctenosaura hemilopha
    Molecular Ecology Notes, 2006
    Co-Authors: M. C. Blázquez, Ricardo Rodríguez Estrella, A. Munguía Vega
    Abstract:

    Ten microsatellite loci were developed for the Baja California spiny-tailed iguana Ctenosaura hemilopha, using an enriched genomic DNA library. In the Cerralvo Island population, seven loci were polymorphic and presented moderate levels of variation. Number of alleles ranged from two to eight (average 4.43), and observed heterozygosity from 0.150 to 0.857 (average 0.492). Polymorphism was detected at six loci on C. hemilopha individuals from the southern Baja California Peninsula. These markers will be useful to study familiar relationships and behaviour on the Cerralvo Island population.

Emily A. Fetzer - One of the best experts on this subject based on the ideXlab platform.

  • Invasive black spiny‐tailed iguanas (Ctenosaura similis) on Gasparilla Island, Florida, USA
    Integrative zoology, 2014
    Co-Authors: Michael L. Avery, Eric A. Tillman, Conny Spurfeld, Richard M. Engeman, Kelin P. Maciejewski, Jessica D. Brown, Emily A. Fetzer
    Abstract:

    The native range of Ctenosaura similis extends from southern Mexico through Panama. From an initial introduction of 3 animals in 1979, the species now numbers in the thousands on Gasparilla Island in southwest Florida. In response to complaints of property damage from residents and threats to native species, local officials and the US Department of Agriculture Wildlife Services began a removal program in 2008. Through 2011, trappers removed 9467 ctenosaurs. The number removed declined from 32 iguanas/day in 2008 to 1.9 iguanas/day in 2011 despite no easing of the control effort. We necropsied 2757 ctenosaurs to document aspects of their natural history. Females outnumbered males overall, although the largest size class (>300 mm snout-vent length) included 32 males and just 2 females. Reproduction was seasonal. We found oviducal eggs in females from early Apr to early Jun, approximately 2 months later than C. similis in its native range. We trapped hatchlings from late Jul to early Oct coincident with the summer rainy season. Clutch size increased with female body size, with 62 being the largest clutch size recorded. In general, the biology of the invasive population on Gasparilla Island resembles native C. similis populations in Central America, except for the lack of large individuals. We suggest that shorter day length and colder temperatures create environmental conditions that are suboptimal for individual growth compared to those in the native range.

  • invasive black spiny tailed iguanas Ctenosaura similis on gasparilla island florida usa
    Integrative Zoology, 2014
    Co-Authors: Michael L. Avery, Eric A. Tillman, Conny Spurfeld, Richard M. Engeman, Kelin P. Maciejewski, Jessica D. Brown, Emily A. Fetzer
    Abstract:

    The native range of Ctenosaura similis extends from southern Mexico through Panama. From an initial introduction of 3 animals in 1979, the species now numbers in the thousands on Gasparilla Island in southwest Florida. In response to complaints of property damage from residents and threats to native species, local officials and the US Department of Agriculture Wildlife Services began a removal program in 2008. Through 2011, trappers removed 9467 ctenosaurs. The number removed declined from 32 iguanas/day in 2008 to 1.9 iguanas/day in 2011 despite no easing of the control effort. We necropsied 2757 ctenosaurs to document aspects of their natural history. Females outnumbered males overall, although the largest size class (>300 mm snout-vent length) included 32 males and just 2 females. Reproduction was seasonal. We found oviducal eggs in females from early Apr to early Jun, approximately 2 months later than C. similis in its native range. We trapped hatchlings from late Jul to early Oct coincident with the summer rainy season. Clutch size increased with female body size, with 62 being the largest clutch size recorded. In general, the biology of the invasive population on Gasparilla Island resembles native C. similis populations in Central America, except for the lack of large individuals. We suggest that shorter day length and colder temperatures create environmental conditions that are suboptimal for individual growth compared to those in the native range.

Larry J. Buckley - One of the best experts on this subject based on the ideXlab platform.

  • Never judge an iguana by its spines: Systematics of the Yucatan spiny tailed iguana, Ctenosaura defensor (Cope, 1866)
    Molecular phylogenetics and evolution, 2017
    Co-Authors: Catherine L. Malone, Victorhugo Reynoso, Larry J. Buckley
    Abstract:

    Spiny tailed iguanas are highly diverse clade of lizards in Mesoamerica, ranging from northern Mexico through Panama. Utilizing 2 regions of mitochondrial DNA (1948bp) and 4 nuclear loci (2232bp) we explored the relationships between these species and the phylogeographic history of the major clades. We discovered that the lineage endemic to the Yucatan Peninsula renders the genus Ctenosaura paraphyletic. To resolve this non-monophyly, we resurrect the taxon Cachryx Cope, 1866, and provide a new diagnosis for the genus. We also find that small body-size and highly spinose tails in the species previously referred to the subgenus Enyaliosaurus, have evolved independently 3 times. Cachryx were recovered as sister to the lineage of iguanines endemic to the Galapagos Islands, and we discuss biogeographic scenarios to explain this relationship as well as those among the primary clades of Ctenosaura in Mesoamerica.

  • Evidence for the Specific Status of the Honduran Lizards Formerly Referred to Ctenosaura palearis (Reptilia: Squamata: Iguanidae)
    Copeia, 1997
    Co-Authors: Larry J. Buckley, Ralph W. Axtell
    Abstract:

    Ctenosaura melanosterna, a new species of spiny-tailed iguana, is described from the Aguan Valley and the Cayos Cochinos in Honduras. Its closest phylogenetic affinities are with C. palearis from the Motagua Valley in Guatemala with which it has previously been considered conspecific. Previous work on relationships within

Xichen Zhang - One of the best experts on this subject based on the ideXlab platform.

  • Identification of a novel amelogenin gene splicing transcript in Ctenosaura similis.
    2013
    Co-Authors: Xinping Wang, Xuliang Deng, Xichen Zhang
    Abstract:

    Chromatograms showed the partial sequence of two Ctenosaura similis amelogenin transcripts identified by sequencing PCR-amplified products. The cDNA clones corresponding to the transcripts were designed as C. similis-T2L (UP) and C. similis-T2S (low), respectively. In relation to C. similis-T2S, C. similis-T2L contains additional nucleotides (UP).

  • Heterogeneous expression of amelogenin in iguana Ctenosaura similis tooth tissue.
    2013
    Co-Authors: Xinping Wang, Xuliang Deng, Xichen Zhang
    Abstract:

    Detection of amelogenin expression in iguana Ctenosaura similis tooth organ using antibody against iguana recombinant amelogenin protein. One major band with molecular weight (MW) of 20 kDa, and two minor bands with MW of 22 kDa and 8 kDa were detected (lane 2), respectively, in relation to proteins marker (lane 1). The non-tooth tissue was used as a negative control (lane 3).

  • Expression of exon X protein in tooth organ. (
    2013
    Co-Authors: Xinping Wang, Xuliang Deng, Xichen Zhang
    Abstract:

    A) The microphotograph illustrates the components of the developing iguana tooth organ, including the enamel layer (en), dental layer (de) and dental pulp (pl). (B) Expression of amelogenin protein isoform containing exon X in the Ctenosaura similis tooth organs by immunohistochemistry staining using antibody against iguana amelogenin exon X peptide. Immunoreaction with antibody against exon X was detected in the enamel layer (en) and dental pulp.

  • Novel iguana amelogenin gene cDNA structure.
    2013
    Co-Authors: Xinping Wang, Xuliang Deng, Xichen Zhang
    Abstract:

    Analysis of novel iguana amelogenin sequence revealed the full-length amelogenin cDNA containing 7 exons including exon 1, 2, 3, 5, X, 6, and 7 (exon numbers is relative to published mammalian amelogenin exon numbers). Different from amelogenin genes of all species so far, an unique exon named exon X between exon 5 and exon 6 was detected; Similar to other species, no corresponding sequence elements were detected resembling exon 4, suggesting that amelogenin exon 4 is skipped or deleted in Ctenosaura similis.

  • Identification of a Novel Splicing Form of Amelogenin Gene in a Reptile, Ctenosaura similis
    PLoS ONE, 2012
    Co-Authors: Xinping Wang, Xuliang Deng, Xichen Zhang
    Abstract:

    Amelogenin, the major enamel matrix protein in tooth development, has been demonstrated to play a significant role in tooth enamel formation. Previous studies have identified the alternative splicing of amelogenin in many mammalian vertebrates as one mechanism for amelogenin heterogeneous expression in teeth. While amelogenin and its splicing forms in mammalian vertebrates have been cloned and sequenced, the amelogenin gene, especially its splicing forms in non-mammalian species, remains largely unknown. To better understand the mechanism underlying amelogenin evolution, we previously cloned and characterized an amelogenin gene sequence from a squamate, the green iguana. In this study, we employed RT-PCR to amplify the amelogenin gene from the black spiny-tailed iguana Ctenosaura similis teeth, and discovered a novel splicing form of the amelogenin gene. The transcript of the newly identified iguana amelogenin gene (named C. Similis-T2L) is 873 nucleotides long encoding an expected polypeptide of 206 amino acids. The C. Similis-T2L contains a unique exon denominated exon X, which is located between exon 5 and exon 6. The C. Similis-T2L contains 7 exons including exon 1, 2, 3, 5, X, 6, and 7. Analysis of the secondary and tertiary structures of T2L amelogenin protein demonstrated that exon X has a dramatic effect on the amelogenin structures. This is the first report to provide definitive evidence for the amelogenin alternative splicing in non-mammalian vertebrates, revealing a unique exon X and the splicing form of the amelogenin gene transcript in Ctenosaura similis.

Michael L. Avery - One of the best experts on this subject based on the ideXlab platform.

  • Invasive black spiny‐tailed iguanas (Ctenosaura similis) on Gasparilla Island, Florida, USA
    Integrative zoology, 2014
    Co-Authors: Michael L. Avery, Eric A. Tillman, Conny Spurfeld, Richard M. Engeman, Kelin P. Maciejewski, Jessica D. Brown, Emily A. Fetzer
    Abstract:

    The native range of Ctenosaura similis extends from southern Mexico through Panama. From an initial introduction of 3 animals in 1979, the species now numbers in the thousands on Gasparilla Island in southwest Florida. In response to complaints of property damage from residents and threats to native species, local officials and the US Department of Agriculture Wildlife Services began a removal program in 2008. Through 2011, trappers removed 9467 ctenosaurs. The number removed declined from 32 iguanas/day in 2008 to 1.9 iguanas/day in 2011 despite no easing of the control effort. We necropsied 2757 ctenosaurs to document aspects of their natural history. Females outnumbered males overall, although the largest size class (>300 mm snout-vent length) included 32 males and just 2 females. Reproduction was seasonal. We found oviducal eggs in females from early Apr to early Jun, approximately 2 months later than C. similis in its native range. We trapped hatchlings from late Jul to early Oct coincident with the summer rainy season. Clutch size increased with female body size, with 62 being the largest clutch size recorded. In general, the biology of the invasive population on Gasparilla Island resembles native C. similis populations in Central America, except for the lack of large individuals. We suggest that shorter day length and colder temperatures create environmental conditions that are suboptimal for individual growth compared to those in the native range.

  • invasive black spiny tailed iguanas Ctenosaura similis on gasparilla island florida usa
    Integrative Zoology, 2014
    Co-Authors: Michael L. Avery, Eric A. Tillman, Conny Spurfeld, Richard M. Engeman, Kelin P. Maciejewski, Jessica D. Brown, Emily A. Fetzer
    Abstract:

    The native range of Ctenosaura similis extends from southern Mexico through Panama. From an initial introduction of 3 animals in 1979, the species now numbers in the thousands on Gasparilla Island in southwest Florida. In response to complaints of property damage from residents and threats to native species, local officials and the US Department of Agriculture Wildlife Services began a removal program in 2008. Through 2011, trappers removed 9467 ctenosaurs. The number removed declined from 32 iguanas/day in 2008 to 1.9 iguanas/day in 2011 despite no easing of the control effort. We necropsied 2757 ctenosaurs to document aspects of their natural history. Females outnumbered males overall, although the largest size class (>300 mm snout-vent length) included 32 males and just 2 females. Reproduction was seasonal. We found oviducal eggs in females from early Apr to early Jun, approximately 2 months later than C. similis in its native range. We trapped hatchlings from late Jul to early Oct coincident with the summer rainy season. Clutch size increased with female body size, with 62 being the largest clutch size recorded. In general, the biology of the invasive population on Gasparilla Island resembles native C. similis populations in Central America, except for the lack of large individuals. We suggest that shorter day length and colder temperatures create environmental conditions that are suboptimal for individual growth compared to those in the native range.

  • Acetaminophen and zinc phosphide for lethal management of invasive lizards Ctenosaura similis
    Current Zoology, 2011
    Co-Authors: Michael L. Avery, John D. Eisemann, Kandy L. Keacher, Peter J. Savarie
    Abstract:

    Reducing populations of invasive lizards through trapping and shooting is feasible in many cases but effective inte- grated management relies on a variety of tools, including toxicants. In Florida, using wild-caught non-native black spiny-tailed iguanas Ctenosaura similis, we screened acetaminophen and zinc phosphide to determine their suitability for effective population management of this prolific invasive species. Of the animals that received acetaminophen, none died except at the highest test dose, 240 mg per lizard, which is not practical for field use. Zinc phosphide produced 100% mortality at dose levels as little as 25 mg per lizard, equivalent to about 0.5% in bait which is lower than currently used in commercial baits for commensal rodent con- trol. We conclude that zinc phosphide has potential as a useful tool for reducing populations of invasive lizards such as the black spiny-tailed iguana provided target-selective delivery methods are developed (Current Zoology 57 (5): 625-629, 2011). Keywords Acetaminophen, Black spiny-tailed iguana, Ctenosaura similis, Florida, Invasive, Zinc phosphide