The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Pamela C. Yelick - One of the best experts on this subject based on the ideXlab platform.
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Advances and perspectives in Tooth Tissue engineering.
Journal of tissue engineering and regenerative medicine, 2016Co-Authors: Nelson Monteiro, Pamela C. YelickAbstract:Bio-engineered teeth that can grow and remodel in a manner similar to that of natural teeth have the potential to serve as permanent replacements to the currently used prosthetic teeth, such as dental implants. A major challenge in designing functional bio-engineered teeth is to mimic both the structural and anisotropic mechanical characteristics of the native Tooth. Therefore, the field of dental and whole Tooth regeneration has advanced towards the molecular and nanoscale design of bio-active, biomimetic systems, using biomaterials, drug delivery systems and stem cells. The focus of this review is to discuss recent advances in Tooth Tissue engineering, using biomimetic scaffolds that provide proper architectural cues, exhibit the capacity to support dental stem cell proliferation and differentiation and sequester and release bio-active agents, such as growth factors and nucleic acids, in a spatiotemporal controlled manner. Although many in vitro and in vivo studies on Tooth regeneration appear promising, before Tooth Tissue engineering becomes a reality for humans, additional research is needed to perfect methods that use adult human dental stem cells, as opposed to embryonic dental stem cells, and to devise the means to generate bio-engineered teeth of predetermined size and shape. Copyright © 2016 John Wiley & Sons, Ltd.
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Tooth Tissue engineering the importance of blood products as a supplement in Tissue culture medium for human pulp dental stem cells
Tissue Engineering Part A, 2015Co-Authors: Ricardo Luiz Pisciolaro, Pamela C. Yelick, Monica Talarico Duailibi, Joseph P Vacanti, Lydia Masako Ferreira, Neil Ferreira Novo, Yara Juliano, Debora Pallos, Silvio Eduardo DuailibiAbstract:One of the goals in using cells for Tissue engineering (TE) and cell therapy consists of optimizing the medium for cell culture. The present study compares three different blood product supplements for improved cell proliferation and protection against DNA damage in cultured human dental pulp stem cells for Tooth TE applications. Human cells from dental pulp were first characterized as adult stem cells (ectomesenchymal mixed origin) by flow cytometry. Next, four different cell culture conditions were tested: I, supplement-free; II, supplemented with fetal bovine serum; III, allogeneic human serum; and IV, autologous human serum. Cultured cells were then characterized for cell proliferation, mineralized nodule formation, and colony-forming units (CFU) capability. After 28 days in culture, the comet assay was performed to assess possible damage in cellular DNA. Our results revealed that Protocol IV achieved higher cell proliferation than Protocol I (p = 0.0112). Protocols II and III resulted in higher cell ...
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Tooth Tissue engineering the importance of blood products as a supplement in Tissue culture medium for human pulp dental stem cells
Tissue Engineering Part A, 2015Co-Authors: Ricardo Luiz Pisciolaro, Pamela C. Yelick, Monica Talarico Duailibi, Neil Ferreira Novo, Yara Juliano, Debora Pallos, Joseph P VacantiAbstract:One of the goals in using cells for Tissue engineering (TE) and cell therapy consists of optimizing the medium for cell culture. The present study compares three different blood product supplements for improved cell proliferation and protection against DNA damage in cultured human dental pulp stem cells for Tooth TE applications. Human cells from dental pulp were first characterized as adult stem cells (ectomesenchymal mixed origin) by flow cytometry. Next, four different cell culture conditions were tested: I, supplement-free; II, supplemented with fetal bovine serum; III, allogeneic human serum; and IV, autologous human serum. Cultured cells were then characterized for cell proliferation, mineralized nodule formation, and colony-forming units (CFU) capability. After 28 days in culture, the comet assay was performed to assess possible damage in cellular DNA. Our results revealed that Protocol IV achieved higher cell proliferation than Protocol I (p = 0.0112). Protocols II and III resulted in higher cell proliferation than Protocol I, but no statistical differences were found relative to Protocol IV. The comet assay revealed less cell damage in cells cultured using Protocol IV as compared to Protocols II and III. The damage percentage observed on Protocol II was significantly higher than all other protocols. CFUs capability was highest using Protocol IV (p = 0.0018) and III, respectively, and the highest degree of mineralization was observed using Protocol IV as compared to Protocols II and III. Protocol IV resulted in significantly improved cell proliferation, and no cell damage was observed. These results demonstrate that human blood product supplements can be used as feasible supplements for culturing adult human dental stem cells.
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Tooth Tissue engineering optimal dental stem cell harvest based on Tooth development
Artificial Organs, 2011Co-Authors: Monica Talarico Duailibi, Silvio Eduardo Duailibi, Joseph P Vacanti, Eduardo Felippe Duailibi Neto, Renata Matalon Negreiros, Waldyr Antonio Jorge, Lydia Masako Ferreira, Pamela C. YelickAbstract:Our long-term objective is to devise reliable methods to generate biological replacement teeth exhibiting the physical properties and functions of naturally formed human teeth. Previously, we demonstrated the successful use of Tissue engineering approaches to generate small, bioengineered Tooth crowns from harvested pig and rat postnatal dental stem cells (DSCs). To facilitate characterizations of human DSCs, we have developed a novel radiographic staging system to accurately correlate human third molar Tooth developmental stage with anticipated harvested DSC yield. Our results demonstrated that DSC yields were higher in less developed teeth (Stages 1 and 2), and lower in more developed teeth (Stages 3, 4, and 5). The greatest cell yields and colony-forming units (CFUs) capability was obtained from Stages 1 and 2 Tooth dental pulp. We conclude that radiographic developmental staging can be used to accurately assess the utility of harvested human teeth for future dental Tissue engineering applications.
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reconstructing mandibular defects using autologous Tissue engineered Tooth and bone constructs
Journal of Oral and Maxillofacial Surgery, 2009Co-Authors: Harutsugi Abukawa, Conan S Young, Joseph P Vacanti, Weibo Zhang, Rose Asrican, Leonard B Kaban, Maria J Troulis, Pamela C. YelickAbstract:Purpose Current strategies for jaw reconstruction require multiple operations to replace bone and teeth. To improve on these methods, we investigated simultaneous mandibular and Tooth reconstruction, using a Yucatan minipig model. Materials and Methods Tooth and bone constructs were prepared from third molar Tooth Tissue and iliac-crest bone marrow-derived osteoblasts isolated from, and implanted back into, the same pig as an autologous reconstruction. Implants were harvested after 12 and 20 weeks and evaluated by x-ray, ultrahigh-resolution volume computed tomographic (VCT), histological, and immunohistochemical analyses. Results Small Tooth structures were identified, and consisted of organized dentin, enamel, pulp, and periodontal ligament Tissues, surrounded by new bone. No dental Tissues formed in implants without Tooth-bud cells, and bone regeneration was observed to a limited extent. Immunohistochemical analyses using Tooth-specific and bone-specific antibodies confirmed the identity of regenerated Tissues. Conclusions This pilot study supports the feasibility of Tissue-engineering approaches for coordinated autologous Tooth and mandible reconstruction, and provides a basis for future improvement of this technique for eventual clinical use in humans.
Christian Cambillau - One of the best experts on this subject based on the ideXlab platform.
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characterization of the porphyromonas gingivalis type ix secretion trans envelope porklmnp core complex
Journal of Biological Chemistry, 2017Co-Authors: Maxence S Vincent, Mickael J Canestrari, Philippe Leone, Julien Stathopulos, Berengere Ize, Abdelrahim Zoued, Christian CambillauAbstract:The transport of proteins at the cell surface of Bacteroidetes depends on a secretory apparatus known as type IX secretion system (T9SS). This machine is responsible for the cell surface exposition of various proteins, such as adhesins, required for gliding motility in Flavobacterium, S-layer components in Tannerella forsythia, and Tooth Tissue-degrading enzymes in the oral pathogen Porphyromonas gingivalis Although a number of subunits of the T9SS have been identified, we lack details on the architecture of this secretion apparatus. Here we provide evidence that five of the genes encoding the core complex of the T9SS are co-transcribed and that the gene products are distributed in the cell envelope. Protein-protein interaction studies then revealed that these proteins oligomerize and interact through a dense network of contacts.
Monica Talarico Duailibi - One of the best experts on this subject based on the ideXlab platform.
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Tooth Tissue engineering the importance of blood products as a supplement in Tissue culture medium for human pulp dental stem cells
Tissue Engineering Part A, 2015Co-Authors: Ricardo Luiz Pisciolaro, Pamela C. Yelick, Monica Talarico Duailibi, Joseph P Vacanti, Lydia Masako Ferreira, Neil Ferreira Novo, Yara Juliano, Debora Pallos, Silvio Eduardo DuailibiAbstract:One of the goals in using cells for Tissue engineering (TE) and cell therapy consists of optimizing the medium for cell culture. The present study compares three different blood product supplements for improved cell proliferation and protection against DNA damage in cultured human dental pulp stem cells for Tooth TE applications. Human cells from dental pulp were first characterized as adult stem cells (ectomesenchymal mixed origin) by flow cytometry. Next, four different cell culture conditions were tested: I, supplement-free; II, supplemented with fetal bovine serum; III, allogeneic human serum; and IV, autologous human serum. Cultured cells were then characterized for cell proliferation, mineralized nodule formation, and colony-forming units (CFU) capability. After 28 days in culture, the comet assay was performed to assess possible damage in cellular DNA. Our results revealed that Protocol IV achieved higher cell proliferation than Protocol I (p = 0.0112). Protocols II and III resulted in higher cell ...
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Tooth Tissue engineering the importance of blood products as a supplement in Tissue culture medium for human pulp dental stem cells
Tissue Engineering Part A, 2015Co-Authors: Ricardo Luiz Pisciolaro, Pamela C. Yelick, Monica Talarico Duailibi, Neil Ferreira Novo, Yara Juliano, Debora Pallos, Joseph P VacantiAbstract:One of the goals in using cells for Tissue engineering (TE) and cell therapy consists of optimizing the medium for cell culture. The present study compares three different blood product supplements for improved cell proliferation and protection against DNA damage in cultured human dental pulp stem cells for Tooth TE applications. Human cells from dental pulp were first characterized as adult stem cells (ectomesenchymal mixed origin) by flow cytometry. Next, four different cell culture conditions were tested: I, supplement-free; II, supplemented with fetal bovine serum; III, allogeneic human serum; and IV, autologous human serum. Cultured cells were then characterized for cell proliferation, mineralized nodule formation, and colony-forming units (CFU) capability. After 28 days in culture, the comet assay was performed to assess possible damage in cellular DNA. Our results revealed that Protocol IV achieved higher cell proliferation than Protocol I (p = 0.0112). Protocols II and III resulted in higher cell proliferation than Protocol I, but no statistical differences were found relative to Protocol IV. The comet assay revealed less cell damage in cells cultured using Protocol IV as compared to Protocols II and III. The damage percentage observed on Protocol II was significantly higher than all other protocols. CFUs capability was highest using Protocol IV (p = 0.0018) and III, respectively, and the highest degree of mineralization was observed using Protocol IV as compared to Protocols II and III. Protocol IV resulted in significantly improved cell proliferation, and no cell damage was observed. These results demonstrate that human blood product supplements can be used as feasible supplements for culturing adult human dental stem cells.
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Tooth Tissue engineering optimal dental stem cell harvest based on Tooth development
Artificial Organs, 2011Co-Authors: Monica Talarico Duailibi, Silvio Eduardo Duailibi, Joseph P Vacanti, Eduardo Felippe Duailibi Neto, Renata Matalon Negreiros, Waldyr Antonio Jorge, Lydia Masako Ferreira, Pamela C. YelickAbstract:Our long-term objective is to devise reliable methods to generate biological replacement teeth exhibiting the physical properties and functions of naturally formed human teeth. Previously, we demonstrated the successful use of Tissue engineering approaches to generate small, bioengineered Tooth crowns from harvested pig and rat postnatal dental stem cells (DSCs). To facilitate characterizations of human DSCs, we have developed a novel radiographic staging system to accurately correlate human third molar Tooth developmental stage with anticipated harvested DSC yield. Our results demonstrated that DSC yields were higher in less developed teeth (Stages 1 and 2), and lower in more developed teeth (Stages 3, 4, and 5). The greatest cell yields and colony-forming units (CFUs) capability was obtained from Stages 1 and 2 Tooth dental pulp. We conclude that radiographic developmental staging can be used to accurately assess the utility of harvested human teeth for future dental Tissue engineering applications.
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bioengineered teeth from cultured rat Tooth bud cells
Journal of Dental Research, 2004Co-Authors: Monica Talarico Duailibi, Silvio Eduardo Duailibi, Conan S Young, John D Bartlett, Joseph P Vacanti, Pamela C. YelickAbstract:The recent bioengineering of complex Tooth structures from pig Tooth bud Tissues suggests the potential for the regeneration of mammalian dental Tissues. We have improved Tooth bioengineering methods by comparing the utility of cultured rat Tooth bud cells obtained from three- to seven-day post-natal (dpn) rats for Tooth-Tissue-engineering applications. Cell-seeded biodegradable scaffolds were grown in the omenta of adult rat hosts for 12 wks, then harvested. Analyses of 12-week implant Tissues demonstrated that dissociated 4-dpn rat Tooth bud cells seeded for 1 hr onto PGA or PLGA scaffolds generated bioengineered Tooth Tissues most reliably. We conclude that Tooth-Tissue-engineering methods can be used to generate both pig and rat Tooth Tissues. Furthermore, our ability to bioengineer Tooth structures from cultured Tooth bud cells suggests that dental epithelial and mesenchymal stem cells can be maintained in vitro for at least 6 days.
Minoru Ueda - One of the best experts on this subject based on the ideXlab platform.
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evaluation of scaffold materials for Tooth Tissue engineering
Journal of Biomedical Materials Research Part A, 2010Co-Authors: Takayuki Ohara, Toshimitsu Itaya, Kazutada Usami, Yusuke Ando, Hiroya Sakurai, Masaki J Honda, Minoru UedaAbstract:Recently, the possibility of Tooth Tissue engineering has been reported. Although there are a number of available materials, information about scaffolds for Tooth Tissue engineering is still limited. To improve the manageability of Tooth Tissue engineering, the effect of scaffolds on in vivo Tooth regeneration was evaluated. Collagen and fibrin were selected for this study based on the biocompatibility to dental papilla-derived cells and the results were compared with those of polyglycolic acid (PGA) fiber and beta-tricalcium phosphate (beta-TCP) porous block, which are commonly used for Tooth, dentin and bone Tissue engineering. Isolated porcine Tooth germ-derived cells were seeded onto one of those scaffolds and transplanted to the back of nude mice. Tooth bud-like structures were observed more frequently in collagen and fibrin gels than on PGA or beta-TCP, while the amount of hard Tissue formation was less. The results showed that collagen and fibrin gel support the initial regeneration process of Tooth buds possibly due to their ability to support the growth of epithelial and mesenchymal cells. On the other hand, maturation of Tooth buds was difficult in fibrin and collagen gels, which may require other factors.
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performance of collagen sponge as a 3 d scaffold for Tooth Tissue engineering
Biomaterials, 2006Co-Authors: Takayuki Ohara, Masaki J Honda, Minoru Ueda, Yoshinori Sumita, Shuhei Tsuchiya, Hiroshi Sagara, Hideaki KagamiAbstract:Abstract Tooth structure can be regenerated by seeding dissociated Tooth cells onto polyglycolic acid fiber mesh, although the success rate of Tooth production is low. The present study was designed to compare the performance of collagen sponge with polyglycolic acid fiber mesh as a 3-D scaffold for Tooth-Tissue engineering. Porcine third molar teeth at the early stage of crown formation were enzymatically dissociated into single cells, and the heterogeneous cells were seeded onto collagen sponge or the polyglycolic acid fiber mesh scaffolds. Scaffolds were then cultured to evaluate cell adhesion and ALP activity in vitro. An in vivo analysis was performed by implanting the constructs into the omentum of immunocompromised rats and evaluating Tooth production up to 25 weeks. After 24 h, there were a significantly higher number of cells attached to the collagen sponge scaffold than the polyglycolic acid fiber mesh scaffold. Similarly, the ALP activity was significantly higher for the collagen sponge scaffold was than the polyglycolic acid fiber mesh scaffold after 7 days of culture. The area of calcified Tissue formed in the collagen sponge scaffold was also larger than in the polyglycolic acid fiber mesh scaffold. The results from in vivo experiments show conclusively that a collagen sponge scaffold allows Tooth production with a higher degree of success than polyglycolic acid fiber mesh. Taken together, the results from this study show that collagen sponge scaffold is superior to the polyglycolic acid fiber mesh scaffold for Tooth-Tissue engineering.
Joseph P Vacanti - One of the best experts on this subject based on the ideXlab platform.
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Tooth Tissue engineering the importance of blood products as a supplement in Tissue culture medium for human pulp dental stem cells
Tissue Engineering Part A, 2015Co-Authors: Ricardo Luiz Pisciolaro, Pamela C. Yelick, Monica Talarico Duailibi, Joseph P Vacanti, Lydia Masako Ferreira, Neil Ferreira Novo, Yara Juliano, Debora Pallos, Silvio Eduardo DuailibiAbstract:One of the goals in using cells for Tissue engineering (TE) and cell therapy consists of optimizing the medium for cell culture. The present study compares three different blood product supplements for improved cell proliferation and protection against DNA damage in cultured human dental pulp stem cells for Tooth TE applications. Human cells from dental pulp were first characterized as adult stem cells (ectomesenchymal mixed origin) by flow cytometry. Next, four different cell culture conditions were tested: I, supplement-free; II, supplemented with fetal bovine serum; III, allogeneic human serum; and IV, autologous human serum. Cultured cells were then characterized for cell proliferation, mineralized nodule formation, and colony-forming units (CFU) capability. After 28 days in culture, the comet assay was performed to assess possible damage in cellular DNA. Our results revealed that Protocol IV achieved higher cell proliferation than Protocol I (p = 0.0112). Protocols II and III resulted in higher cell ...
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Tooth Tissue engineering the importance of blood products as a supplement in Tissue culture medium for human pulp dental stem cells
Tissue Engineering Part A, 2015Co-Authors: Ricardo Luiz Pisciolaro, Pamela C. Yelick, Monica Talarico Duailibi, Neil Ferreira Novo, Yara Juliano, Debora Pallos, Joseph P VacantiAbstract:One of the goals in using cells for Tissue engineering (TE) and cell therapy consists of optimizing the medium for cell culture. The present study compares three different blood product supplements for improved cell proliferation and protection against DNA damage in cultured human dental pulp stem cells for Tooth TE applications. Human cells from dental pulp were first characterized as adult stem cells (ectomesenchymal mixed origin) by flow cytometry. Next, four different cell culture conditions were tested: I, supplement-free; II, supplemented with fetal bovine serum; III, allogeneic human serum; and IV, autologous human serum. Cultured cells were then characterized for cell proliferation, mineralized nodule formation, and colony-forming units (CFU) capability. After 28 days in culture, the comet assay was performed to assess possible damage in cellular DNA. Our results revealed that Protocol IV achieved higher cell proliferation than Protocol I (p = 0.0112). Protocols II and III resulted in higher cell proliferation than Protocol I, but no statistical differences were found relative to Protocol IV. The comet assay revealed less cell damage in cells cultured using Protocol IV as compared to Protocols II and III. The damage percentage observed on Protocol II was significantly higher than all other protocols. CFUs capability was highest using Protocol IV (p = 0.0018) and III, respectively, and the highest degree of mineralization was observed using Protocol IV as compared to Protocols II and III. Protocol IV resulted in significantly improved cell proliferation, and no cell damage was observed. These results demonstrate that human blood product supplements can be used as feasible supplements for culturing adult human dental stem cells.
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Tooth Tissue engineering optimal dental stem cell harvest based on Tooth development
Artificial Organs, 2011Co-Authors: Monica Talarico Duailibi, Silvio Eduardo Duailibi, Joseph P Vacanti, Eduardo Felippe Duailibi Neto, Renata Matalon Negreiros, Waldyr Antonio Jorge, Lydia Masako Ferreira, Pamela C. YelickAbstract:Our long-term objective is to devise reliable methods to generate biological replacement teeth exhibiting the physical properties and functions of naturally formed human teeth. Previously, we demonstrated the successful use of Tissue engineering approaches to generate small, bioengineered Tooth crowns from harvested pig and rat postnatal dental stem cells (DSCs). To facilitate characterizations of human DSCs, we have developed a novel radiographic staging system to accurately correlate human third molar Tooth developmental stage with anticipated harvested DSC yield. Our results demonstrated that DSC yields were higher in less developed teeth (Stages 1 and 2), and lower in more developed teeth (Stages 3, 4, and 5). The greatest cell yields and colony-forming units (CFUs) capability was obtained from Stages 1 and 2 Tooth dental pulp. We conclude that radiographic developmental staging can be used to accurately assess the utility of harvested human teeth for future dental Tissue engineering applications.
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reconstructing mandibular defects using autologous Tissue engineered Tooth and bone constructs
Journal of Oral and Maxillofacial Surgery, 2009Co-Authors: Harutsugi Abukawa, Conan S Young, Joseph P Vacanti, Weibo Zhang, Rose Asrican, Leonard B Kaban, Maria J Troulis, Pamela C. YelickAbstract:Purpose Current strategies for jaw reconstruction require multiple operations to replace bone and teeth. To improve on these methods, we investigated simultaneous mandibular and Tooth reconstruction, using a Yucatan minipig model. Materials and Methods Tooth and bone constructs were prepared from third molar Tooth Tissue and iliac-crest bone marrow-derived osteoblasts isolated from, and implanted back into, the same pig as an autologous reconstruction. Implants were harvested after 12 and 20 weeks and evaluated by x-ray, ultrahigh-resolution volume computed tomographic (VCT), histological, and immunohistochemical analyses. Results Small Tooth structures were identified, and consisted of organized dentin, enamel, pulp, and periodontal ligament Tissues, surrounded by new bone. No dental Tissues formed in implants without Tooth-bud cells, and bone regeneration was observed to a limited extent. Immunohistochemical analyses using Tooth-specific and bone-specific antibodies confirmed the identity of regenerated Tissues. Conclusions This pilot study supports the feasibility of Tissue-engineering approaches for coordinated autologous Tooth and mandible reconstruction, and provides a basis for future improvement of this technique for eventual clinical use in humans.
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bioengineered teeth from cultured rat Tooth bud cells
Journal of Dental Research, 2004Co-Authors: Monica Talarico Duailibi, Silvio Eduardo Duailibi, Conan S Young, John D Bartlett, Joseph P Vacanti, Pamela C. YelickAbstract:The recent bioengineering of complex Tooth structures from pig Tooth bud Tissues suggests the potential for the regeneration of mammalian dental Tissues. We have improved Tooth bioengineering methods by comparing the utility of cultured rat Tooth bud cells obtained from three- to seven-day post-natal (dpn) rats for Tooth-Tissue-engineering applications. Cell-seeded biodegradable scaffolds were grown in the omenta of adult rat hosts for 12 wks, then harvested. Analyses of 12-week implant Tissues demonstrated that dissociated 4-dpn rat Tooth bud cells seeded for 1 hr onto PGA or PLGA scaffolds generated bioengineered Tooth Tissues most reliably. We conclude that Tooth-Tissue-engineering methods can be used to generate both pig and rat Tooth Tissues. Furthermore, our ability to bioengineer Tooth structures from cultured Tooth bud cells suggests that dental epithelial and mesenchymal stem cells can be maintained in vitro for at least 6 days.
