The Experts below are selected from a list of 234 Experts worldwide ranked by ideXlab platform
Yae Zhao - One of the best experts on this subject based on the ideXlab platform.
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Study on the Relationship Between Microbial Composition and Living Environment in Important Medical Mites Based on Illumina MiSeq Sequencing Technology.
Journal of Medical Entomology, 2020Co-Authors: Yijie Guo, Rui-ling Wang, Yae Zhao, Dongling Niu, Xiaojuan GongAbstract:The microbiota of mites is closely related to their growth, development, and pathogenicity. Therefore, it is necessary to study the bacteria in mites. Here, for the first time, based on 16s rRNA V3-V4 region, the microbiota of 45 samples of nine species in six families of medically important mites were analyzed using Illumina MiSeq sequencing technique. The results showed that, at the phylum level, Proteobacteria (56.20-86.40%) were the dominant, followed by Firmicutes (6.41-19.43%), Bacteroidetes (5.56-13.38%) and Actinobacteria (1.93-28.07%). But at the genera the microbiota of mites are different, showing four characteristics: 1) The microbiota is related to the parasitic host. Demodex folliculorum (Acariforms: Demodicidae) and D. brevis (Acariforms: Demodicidae), both parasitizing humans, showed similar microbial composition, as did D. canis (Acariforms: Demodicidae) and Sarcoptes scabiei canis (Acariforms: Sarcoptidae) parasitizing dogs, but D. caprae (Acariforms: Demodicidae) parasitizing sheep showed unique microbial community; 2) The microbiota is related to mite's species. Dermatophagoides farinae and Cheyletus malaccensis (Acariforms: Cheyletidae), both collecting from flour, show respective microbial composition; 3) The microbiota is related to the life stage. There were differences in microbiota between adults and larvae of D. farinae, but no differences observed in Psoroptes cuniculi (Acariforms: Psoroptidae); and 4) The microbiota is related to the blood-feeding state. The microbiota of blood-fed Ornithonyssus bacoti (Parasitiformes: Macronyssidae) adults was significantly higher than that of unfed adults. This indicates that the microbiota of mites is affected by mite species, parasitic host, growth stage and habitat. Therefore, understanding these influencing factors will have a very important guiding significance for the prevention and control of mite-borne diseases.
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LSU rDNA D5 region: the DNA barcode for molecular classification and identification of Demodex.
Genome, 2019Co-Authors: Yae Zhao, Dongling Niu, Yuanjun Yang, Rui YangAbstract:Whether ribosomal genes can be used as DNA barcodes for molecular identification of Demodex (Acariformes: Demodicidae) is unclear. To examine this, Demodex folliculorum, D. brevis, D. canis, and D....
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Discrimination between Demodex folliculorum (Acari: Demodicidae) isolates from China and Spain based on mitochondrial cox1 sequences
Journal of Zhejiang University. Science. B, 2013Co-Authors: Yae Zhao, Manuel RojasAbstract:For a long time, classification of Demodex mites has been based mainly on their hosts and phenotypic characteristics. A new subspecies of Demodex folliculorum has been proposed, but not confirmed. Here, cox1 partial sequences of nine isolates of three Demodex species from two geographical sources (China and Spain) were studied to conduct molecular identification of D. folliculorum. Sequencing showed that the mitochondrial cox1 fragments of five D. folliculorum isolates from the facial skin of Chinese individuals were 429 bp long and that their sequence identity was 97.4%. The average sequence divergence was 1.24% among the five Chinese isolates, 0.94% between the two geographical isolate groups (China (5) and Spain (1)), and 2.15% between the two facial tissue sources (facial skin (6) and eyelids (1)). The genetic distance and rate of third-position nucleotide transition/transversion were 0.0125, 2.7 (3/1) among the five Chinese isolates, 0.0094, 3.1 (3/1) between the two geographical isolate groups, and 0.0217, 4.4 (3/1) between the two facial tissue sources. Phylogenetic trees showed that D. folliculorum from the two geographical isolate groups did not form sister clades, while those from different facial tissue sources did. According to the molecular characteristics, it appears that subspecies differentiation might not have occurred and that D. folliculorum isolates from the two geographical sources are of the same population. However, population differentiation might be occurring between isolates from facial skin and eyelids.
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Molecular identification of four phenotypes of human Demodex mites (Acari: Demodicidae) based on mitochondrial 16S rDNA.
Parasitology research, 2013Co-Authors: Yae ZhaoAbstract:Classification of Demodex mites has long depended on hosts and morphological characteristics. However, the fact that two species coexist in the same host and phenotype is easily influenced by environment causes difficulty and indeterminacy in traditional classification. Genotype, which directly reflects the molecular structure characteristics, is relatively stable. In this study, species identification of four phenotypes of human Demodex mites was conducted. Mites were morphologically classified into four phenotypes: long- and short-bodied Demodex folliculorum with finger-like terminus and Demodex brevis with finger- or cone-like terminus. The mitochondrial 16S ribosomal DNA (rDNA) fragment of individual mite was amplified, cloned, sequenced, and aligned. Sequence divergences, genetic distances, transition/transversion rates, and phylogenetic trees were analyzed. The results demonstrated that the 16S rDNA sequence of three phenotypes with finger-like terminus was 337 bp, and that of phenotype with cone-like terminus was 342 bp. The divergences, genetic distances, and transition/transversion rates among the three phenotypes with finger-like terminus were 0.0–2.7 %, 0.000–0.029, and 5.0–7/0 (5/1–7/0), respectively, indicating an intraspecific variation. Yet, those between these three phenotypes and the one with cone-like terminus were 21.6–22.8 %, 2.510–2.589, and 0.47–0.59 (22/47–27/46), respectively, suggesting an interspecific variation. The five phylogenetic trees showed that the three phenotypes with finger-like terminus clustered into one branch, while the phenotype with cone-like terminus clustered into another. In conclusion, terminus is a major morphological characteristic for the identification of human Demodex species. The three phenotypes with finger-like terminus belong to D. folliculorum, while the phenotype with cone-like terminus belongs to D. brevis. Molecular identification can verify and replenish morphological identification.
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rapd scar marker and genetic relationship analysis of three demodex species acari Demodicidae
Parasitology Research, 2012Co-Authors: Yae ZhaoAbstract:For a long time, classification of Demodex mites has been mainly based on their hosts and phenotype characteristics. The study was the first to conduct molecular identification and genetic relationship analysis for six isolates of three Demodex species by random amplified polymorphic DNA (RAPD) and sequence-characterized amplified region (SCAR) marker. Totally, 239 DNA fragments were amplified from six Demodex isolates with 10 random primers in RAPD, of which 165 were polymorphic. Using a single primer, at least five fragments and at most 40 in the six isolates were amplified, whereas within a single isolate, a range of 35–49 fragments were amplified. DNA fingerprints of primers CZ 1–9 revealed intra- and interspecies difference in six Demodex isolates, whereas primer CZ 10 only revealed interspecies difference. The genetic distance and dendrogram showed the intraspecific genetic distances were closer than the interspecific genetic distances. The interspecific genetic distances of Demodex folliculorum and Demodex canis (0.7931–0.8140) were shorter than that of Demodex brevis and D. canis (0.8182–0.8987). The RAPD–SCAR marker displayed primer CZ 10 could be applied to identify the three Demodex species. The 479-bp fragment was specific for D. brevis, and the 261-bp fragment was specific for D. canis. The conclusion was that the RAPD–SCAR multi-marker was effective in molecular identification of three Demodex species. The genetic relationship between D. folliculorum and D. canis was nearer than that between D. folliculorum and D. brevis.
Joanna N. Izdebska - One of the best experts on this subject based on the ideXlab platform.
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Demodex phocidi (Acariformes: Demodecidae) from Phoca vitulina (Carnivora: Phocidae) – the second observation in the world and a supplement to the species description
Oceanological and Hydrobiological Studies, 2020Co-Authors: Joanna N. Izdebska, Leszek Rolbiecki, Karolina Cierocka, Iwona PawliczkaAbstract:Abstract The present study describes a finding of the demodecid mite Demodex phocidi in the seal Phoca vitulina in the Baltic Sea. This is the first identification in Europe and the second in the world. This is also the first observation of the Demodecidae family in the pinnipeds outside North America. A high density of demodecid mites was observed in the skin of the examined seal, but no symptoms of parasitosis were observed. Our findings also supplement the taxonomic description and morphometry of D. phocidi.
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Morphological and ontogenetic characteristics of Demodex plecoti sp. nov. (Acariformes: Demodecidae) from the brown long-eared bat Plecotus auritus (Chiroptera: Vespertilionidae), with comments on parasitism
Systematic and Applied Acarology, 2019Co-Authors: Joanna N. Izdebska, Leszek Rolbiecki, Łukasz Mierzyński, Konrad BidzińskiAbstract:A new species, Demodex plecotisp. nov. (Acariformes: Demodecidae), is described based on adult and immature stages from the brown long-eared bat Plecotus auritus (Linnaeus, 1758) in Poland. The mites were found in 100% of the examined bat individuals, with a mean intensity of 23.5 parasites per host. Demodex plecotisp. nov. was observed only in the hairless body regions, were the skin was membranous, thin and delicate.
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Demodex foveolator (Acariformes: Demodecidae) from Crocidura suaveolens (Soricomorpha: Soricidae) – the second observation worldwide, and a checklist of the demodecid mites of soricomorphs
Annals of parasitology, 2019Co-Authors: Karolina Cierocka, Joanna N. Izdebska, Leszek RolbieckiAbstract:Thus far, only six species of parasitic mites of the Demodecidae family have been recorded in hosts from the Soricomorpha: two from the common shrew (Sorex araneus), two from the Mediterranean water shrew (Neomys anomalus), one from the lesser white-toothed shrew (Crocidura suaveolens) and one from the European mole (Talpa europaea). The present study describes the identification of Demodex foveolator in C. suaveolens from the area of Poland (the Wielkopolska region). This is only the second record of this species in the world, a new mite species for the Polish fauna and a new host record in Poland, i.e. the first identification of Demodecidae in Crocidura mammals.
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Demodex melesinus (Acariformes: Demodecidae) — the forgotten European badger parasite, rediscovered after 100 years
Acta Parasitologica, 2018Co-Authors: Joanna N. Izdebska, Leszek Rolbiecki, Karolina Cierocka, Paulina Kozina, Marta Kolodziej-sobocinskaAbstract:Among 15 demodecid mite species (Acariformes: Demodecidae) recorded from carnivorans Carnivora, 3 species were described from mustelids Mustelidae. They are known only from single records, for which Demodex erminae has been described from the stoat Mustela erminea from Great Britain and New Zealand, D. melesinus from the European badger Meles meles known solely from Great Britain and D. lutrae discovered in the Eurasian otter Lutra lutra from Poland. The current record confirms the existence of D. melesinus , in badger from Poland, after close to one hundred years from its original description, as well as the first detection of the male for this species.
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The status of Demodex cornei: description of the species and developmental stages, and data on demodecid mites in the domestic dog Canis lupus familiaris.
Medical and Veterinary Entomology, 2018Co-Authors: Joanna N. Izdebska, Leszek RolbieckiAbstract:Demodecosis canina is one of the most important dog parasitoses, but its aetiology is still not well known. There are currently two known species of demodecid mite specific to the domestic dog Canis lupus familiaris Linnaeus, 1758 (Carnivora: Canidae). These are Demodex canis Leydig, 1859 (Acariformes: Demodecidae) and Demodex injai Desch & Hillier, 2003. There have also been many reports of the so‐called ‘short form’, considered to be a separate species functioning under the name Demodex cornei nomen nudum, for which, however, no formal valid description has been documented. Taxonomic analysis of short forms of dog demodecid mites, associated with the stratum corneum, was performed, in line with the taxonomic criteria of Demodecidae systematics. This form was found to be a distinct species with features that differ from those of the other known species of this family. The species, including the adult and immature stages, is described. It is likely that different Demodex species parasitizing the domestic dog may be responsible for differentiated symptoms and different courses of demodecosis. However, the basis for clarifying this issue should be the correct, unambiguous identification of the species causing parasitosis.
Leszek Rolbiecki - One of the best experts on this subject based on the ideXlab platform.
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Demodex phocidi (Acariformes: Demodecidae) from Phoca vitulina (Carnivora: Phocidae) – the second observation in the world and a supplement to the species description
Oceanological and Hydrobiological Studies, 2020Co-Authors: Joanna N. Izdebska, Leszek Rolbiecki, Karolina Cierocka, Iwona PawliczkaAbstract:Abstract The present study describes a finding of the demodecid mite Demodex phocidi in the seal Phoca vitulina in the Baltic Sea. This is the first identification in Europe and the second in the world. This is also the first observation of the Demodecidae family in the pinnipeds outside North America. A high density of demodecid mites was observed in the skin of the examined seal, but no symptoms of parasitosis were observed. Our findings also supplement the taxonomic description and morphometry of D. phocidi.
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Morphological and ontogenetic characteristics of Demodex plecoti sp. nov. (Acariformes: Demodecidae) from the brown long-eared bat Plecotus auritus (Chiroptera: Vespertilionidae), with comments on parasitism
Systematic and Applied Acarology, 2019Co-Authors: Joanna N. Izdebska, Leszek Rolbiecki, Łukasz Mierzyński, Konrad BidzińskiAbstract:A new species, Demodex plecotisp. nov. (Acariformes: Demodecidae), is described based on adult and immature stages from the brown long-eared bat Plecotus auritus (Linnaeus, 1758) in Poland. The mites were found in 100% of the examined bat individuals, with a mean intensity of 23.5 parasites per host. Demodex plecotisp. nov. was observed only in the hairless body regions, were the skin was membranous, thin and delicate.
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Demodex foveolator (Acariformes: Demodecidae) from Crocidura suaveolens (Soricomorpha: Soricidae) – the second observation worldwide, and a checklist of the demodecid mites of soricomorphs
Annals of parasitology, 2019Co-Authors: Karolina Cierocka, Joanna N. Izdebska, Leszek RolbieckiAbstract:Thus far, only six species of parasitic mites of the Demodecidae family have been recorded in hosts from the Soricomorpha: two from the common shrew (Sorex araneus), two from the Mediterranean water shrew (Neomys anomalus), one from the lesser white-toothed shrew (Crocidura suaveolens) and one from the European mole (Talpa europaea). The present study describes the identification of Demodex foveolator in C. suaveolens from the area of Poland (the Wielkopolska region). This is only the second record of this species in the world, a new mite species for the Polish fauna and a new host record in Poland, i.e. the first identification of Demodecidae in Crocidura mammals.
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Demodex melesinus (Acariformes: Demodecidae) — the forgotten European badger parasite, rediscovered after 100 years
Acta Parasitologica, 2018Co-Authors: Joanna N. Izdebska, Leszek Rolbiecki, Karolina Cierocka, Paulina Kozina, Marta Kolodziej-sobocinskaAbstract:Among 15 demodecid mite species (Acariformes: Demodecidae) recorded from carnivorans Carnivora, 3 species were described from mustelids Mustelidae. They are known only from single records, for which Demodex erminae has been described from the stoat Mustela erminea from Great Britain and New Zealand, D. melesinus from the European badger Meles meles known solely from Great Britain and D. lutrae discovered in the Eurasian otter Lutra lutra from Poland. The current record confirms the existence of D. melesinus , in badger from Poland, after close to one hundred years from its original description, as well as the first detection of the male for this species.
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The status of Demodex cornei: description of the species and developmental stages, and data on demodecid mites in the domestic dog Canis lupus familiaris.
Medical and Veterinary Entomology, 2018Co-Authors: Joanna N. Izdebska, Leszek RolbieckiAbstract:Demodecosis canina is one of the most important dog parasitoses, but its aetiology is still not well known. There are currently two known species of demodecid mite specific to the domestic dog Canis lupus familiaris Linnaeus, 1758 (Carnivora: Canidae). These are Demodex canis Leydig, 1859 (Acariformes: Demodecidae) and Demodex injai Desch & Hillier, 2003. There have also been many reports of the so‐called ‘short form’, considered to be a separate species functioning under the name Demodex cornei nomen nudum, for which, however, no formal valid description has been documented. Taxonomic analysis of short forms of dog demodecid mites, associated with the stratum corneum, was performed, in line with the taxonomic criteria of Demodecidae systematics. This form was found to be a distinct species with features that differ from those of the other known species of this family. The species, including the adult and immature stages, is described. It is likely that different Demodex species parasitizing the domestic dog may be responsible for differentiated symptoms and different courses of demodecosis. However, the basis for clarifying this issue should be the correct, unambiguous identification of the species causing parasitosis.
J. Cano - One of the best experts on this subject based on the ideXlab platform.
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Demodectic mange in a white-tailed deer from Walker County, Texas.
Journal of medical entomology, 2008Co-Authors: J. C. Turner, J. CanoAbstract:The first documented occurrence of demodectic (Acari: Prostigmata: Demodicidae) mange in white-tailed deer, Odocoileus virginianus Zimmerman, in Texas was diagnosed from deep skin scrapings taken from the neck, shoulders, and withers of a fair-to-poor condition 6 yr-old male deer captured at Elkins Lake (latitude 30° 39′ 27″ N, longitude 95° 32′ 33″ W), Walker County. Approximately 35% of the animal’s skin showed effects of the demodicosis, which may have contributed to the animal’s body condition. The hematology of the infected deer seemed little altered from mean erythrocyte and leukocyte values of the local deer population, except for an observed eosinophilia (23% increase), which is consistent with an ectoparasitic infection and the concomitant bacterial infection observed. Predicated on host specificity and total body length measurements, Demodex odocoilei Desch & Nutting is probably the parasite collected from the captured white-tailed deer.
J. C. Turner - One of the best experts on this subject based on the ideXlab platform.
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Demodectic mange in a white-tailed deer from Walker County, Texas.
Journal of medical entomology, 2008Co-Authors: J. C. Turner, J. CanoAbstract:The first documented occurrence of demodectic (Acari: Prostigmata: Demodicidae) mange in white-tailed deer, Odocoileus virginianus Zimmerman, in Texas was diagnosed from deep skin scrapings taken from the neck, shoulders, and withers of a fair-to-poor condition 6 yr-old male deer captured at Elkins Lake (latitude 30° 39′ 27″ N, longitude 95° 32′ 33″ W), Walker County. Approximately 35% of the animal’s skin showed effects of the demodicosis, which may have contributed to the animal’s body condition. The hematology of the infected deer seemed little altered from mean erythrocyte and leukocyte values of the local deer population, except for an observed eosinophilia (23% increase), which is consistent with an ectoparasitic infection and the concomitant bacterial infection observed. Predicated on host specificity and total body length measurements, Demodex odocoilei Desch & Nutting is probably the parasite collected from the captured white-tailed deer.