The Experts below are selected from a list of 261 Experts worldwide ranked by ideXlab platform
Arnaldo Zaha - One of the best experts on this subject based on the ideXlab platform.
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proteomics analysis of Echinococcus granulosus protoscolex stage
Veterinary Parasitology, 2016Co-Authors: Christian Hidalgo, Arnaldo Zaha, Maria Pia Garcia, Caroll Stoore, Juan Pablo Ramirez, Karina Mariante Monteiro, Ulf Hellman, Henrique Bunselmeyer Ferreira, Norbel Galanti, Eduardo LandererAbstract:Echinococcus granulosus protoscolex proteins were separated using two-dimensional electrophoresis and then identified using mass spectrometry; we identified 61 proteins, 28 which are newly described of which 4 could be involved in hydatid cyst fertility molecular mechanisms.
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Echinococcus ortleppi (G5) and Echinococcus granulosus sensu stricto (G1) loads in cattle from Southern Brazil
Veterinary Parasitology, 2012Co-Authors: Helier Balbinotti, Guilherme B. Santos, Jeferson Badaraco, Ana C. Arend, Daniel ângelo S. Graichen, Karen L. Haag, Arnaldo ZahaAbstract:Echinococcus granulosus sensu stricto (G1) and Echinococcus ortleppi (G5) are haplotypes of the parasite formerly known as Echinococcus granulosus sensu lato, which in its larval stage causes cystic hydatid disease, endemic in Southern Brazil. Epidemiological and molecular knowledge about the haplotypes occurring in a region is essential to control the spread of the disease. The aim of this work was to analyze the haplotype frequency and fertility of hydatid cysts in cattle from the state of Rio Grande do Sul. Cysts were collected and classified according to their fertility status. DNA was extracted from protoscoleces and germinal layers and then used as template for the amplification of the cytochrome c oxidase subunit 1 gene by PCR. Amplicons were purified and sequenced, and the sequences were analyzed for haplotype identification. A total of 638 fertile cysts collected in the last ten years were genotyped. On average, G1 (56.6%) was more frequent than G5 (43.4%). In lungs, the G5 haplotype exhibited a higher parasite load (52.8%), whereas in the liver, G1 was more frequent (90.4%). The analysis revealed an increase in the frequency of G5 haplotype cysts during the period of sampling, and an increase in the abundance of fertile cysts has also been observed in the last several years. Most infertile cysts were genotyped as G1. The possible factors involved in the increase in the proportion of E. ortleppi (G5) and the consequences of this increase are discussed. This study suggests that the proportion of E. ortleppi (G5) loads in cattle may be increasing overtime. © 2012 Elsevier B.V.
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DNA damage, RAD9 and fertility/infertility of Echinococcus granulosus hydatid cysts
Journal of Cellular Physiology, 2008Co-Authors: Gonzalo Cabrera, Arnaldo Zaha, Ulf Hellman, María Eugenia Cabrejos, Alessandra L. Morassutti, Carolina Cabezón, Juana Orellana, Norbel GalantiAbstract:Hydatidosis, caused by the larval stage of the platyhelminth parasite Echinococcus granulosus, affects human and animal health. Hydatid fertile cysts are formed in intermediate hosts (human and her ...
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Echinococcus granulosus: molecular cloning and phylogenetic analysis of an inducible glutathione S-transferase.
Experimental Parasitology, 2000Co-Authors: V. Fernández, Arnaldo Zaha, Cora Chalar, Claudio Martínez, Héctor Musto, Cecilia FernándezAbstract:Abstract Fernandez, V., Chalar, C., Martinez, C., Musto, H., Zaha, A., and Fernandez, C. 2000. Echinococcus granulosus: Molecular cloning and phylogenetic analysis of an inducible glutathione S-transferase. Experimental Parasitology96, 190–194.
Guangyou Yang - One of the best experts on this subject based on the ideXlab platform.
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characterization of a secretory annexin in Echinococcus granulosus
American Journal of Tropical Medicine and Hygiene, 2016Co-Authors: Xingju Song, Dandan Hu, Xiuqin Zhong, Ning Wang, Xiaobin Gu, Tao Wang, Xuerong Peng, Guangyou YangAbstract:Abstract Cystic echinococcosis, caused by Echinococcus granulosus, is a widespread parasitic zoonosis causing economic loss and public health problems. Annexins are important proteins usually present in the plasma membrane, but previous studies have shown that an annexin B33 protein of E. granulosus (Eg-ANX) could be detected in the excretory/secretory products and cyst fluid. In this study, we cloned and characterized Eg-ANX. In silico analysis showed that the amino acid sequence of Eg-ANX was conserved and lacked any signal peptides. The phospholipid-binding activity of recombinant Eg-ANX (rEg-ANX) was tested; liposomes could bind to rEg-ANX only in the presence of Ca2+. In addition, we performed western blotting and immunohistochemical analyses to further validate the secretory properties of Eg-ANX. The protein could be detected in the cyst fluid of E. granulosus and was also present in the intermediate host tissues, which suggested that Eg-ANX might play an important role in parasite–host interaction.
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Molecular insights into a tetraspanin in the hydatid tapeworm Echinococcus granulosus.
Parasites & Vectors, 2015Co-Authors: Dandan Hu, Xingju Song, Xiuqin Zhong, Ning Wang, Yu Zheng, Xiaobin Gu, Tao Wang, Xuerong Peng, Guangyou YangAbstract:Background Cystic echinococcosis (hydatid disease), caused by the tapeworm Echinococcus granulosus (class Cestoda; family Taeniidae), is a neglected tropical disease that results in morbidity and mortality in millions of humans, as well as in huge economic losses in the livestock industry globally. Proteins from the tetraspanin family in parasites have recently become regarded as crucial molecules in interaction with hosts in parasitism and are therefore suitable for the development of vaccines and diagnostic agents. However, no information is available to date on E. granulosus tetraspanin.
Hui Kang - One of the best experts on this subject based on the ideXlab platform.
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the genome of the hydatid tapeworm Echinococcus granulosus
Nature Genetics, 2013Co-Authors: Huajun Zheng, Wenbao Zhang, L Zhang, Zhuangzhi Zhang, Jun Li, Gang Lu, Yuezhu Wang, Yin Huang, Hui KangAbstract:Shengyue Wang and colleagues report the draft genome sequence and transcriptome analysis for Echinococcus granulosus, a parasitic helminth and cause of human hydatid disease. Their comparative genomic analysis identifies genes acquired by E. granulosus that are associated with host immune response, parasite survival and growth.
L Zhang - One of the best experts on this subject based on the ideXlab platform.
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the genome of the hydatid tapeworm Echinococcus granulosus
Nature Genetics, 2013Co-Authors: Huajun Zheng, Wenbao Zhang, L Zhang, Zhuangzhi Zhang, Jun Li, Gang Lu, Yuezhu Wang, Yin Huang, Hui KangAbstract:Shengyue Wang and colleagues report the draft genome sequence and transcriptome analysis for Echinococcus granulosus, a parasitic helminth and cause of human hydatid disease. Their comparative genomic analysis identifies genes acquired by E. granulosus that are associated with host immune response, parasite survival and growth.
Xingju Song - One of the best experts on this subject based on the ideXlab platform.
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characterization of a secretory annexin in Echinococcus granulosus
American Journal of Tropical Medicine and Hygiene, 2016Co-Authors: Xingju Song, Dandan Hu, Xiuqin Zhong, Ning Wang, Xiaobin Gu, Tao Wang, Xuerong Peng, Guangyou YangAbstract:Abstract Cystic echinococcosis, caused by Echinococcus granulosus, is a widespread parasitic zoonosis causing economic loss and public health problems. Annexins are important proteins usually present in the plasma membrane, but previous studies have shown that an annexin B33 protein of E. granulosus (Eg-ANX) could be detected in the excretory/secretory products and cyst fluid. In this study, we cloned and characterized Eg-ANX. In silico analysis showed that the amino acid sequence of Eg-ANX was conserved and lacked any signal peptides. The phospholipid-binding activity of recombinant Eg-ANX (rEg-ANX) was tested; liposomes could bind to rEg-ANX only in the presence of Ca2+. In addition, we performed western blotting and immunohistochemical analyses to further validate the secretory properties of Eg-ANX. The protein could be detected in the cyst fluid of E. granulosus and was also present in the intermediate host tissues, which suggested that Eg-ANX might play an important role in parasite–host interaction.
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Molecular insights into a tetraspanin in the hydatid tapeworm Echinococcus granulosus.
Parasites & Vectors, 2015Co-Authors: Dandan Hu, Xingju Song, Xiuqin Zhong, Ning Wang, Yu Zheng, Xiaobin Gu, Tao Wang, Xuerong Peng, Guangyou YangAbstract:Background Cystic echinococcosis (hydatid disease), caused by the tapeworm Echinococcus granulosus (class Cestoda; family Taeniidae), is a neglected tropical disease that results in morbidity and mortality in millions of humans, as well as in huge economic losses in the livestock industry globally. Proteins from the tetraspanin family in parasites have recently become regarded as crucial molecules in interaction with hosts in parasitism and are therefore suitable for the development of vaccines and diagnostic agents. However, no information is available to date on E. granulosus tetraspanin.
