The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Jingyu Wang - One of the best experts on this subject based on the ideXlab platform.
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autophagy benefits the replication of Egg Drop Syndrome virus in duck embryo fibroblasts
Frontiers in Microbiology, 2018Co-Authors: Xueping Wang, Bo Yang, Shuying Chen, Jingyu WangAbstract:Egg Drop Syndrome virus (EDSV) is an economically important pathogen with a broad host range, and it causes disease that leads to markedly decreased Egg production. Although EDSV is known to induce apoptosis in duck embryo fibroblasts (DEFs), the interaction between EDSV and its host needs to be further researched. Here, we provide the first evidence that EDSV infection triggers autophagy in DEFs through increases in autophagosome-like double-membrane vesicles, the conversion of LC3-I to LC3-II, and LC3 colocalization with viral hexon proteins. Conversely, P62/SQSTM1 degradation, LC3-II turnover, and colocalization of LAMP and LC3 confirmed that EDSV infection triggers complete autophagy. Furthermore, we demonstrated that inhibition of autophagy by chloroquine (CQ) and 3-methyladenine (3MA) or RNA interference targeting ATG-7 decreased the yield of EDSV progeny. In contrast, induction of autophagy by rapamycin increased the EDSV progeny yield. In addition, we preliminarily demonstrated that the class I phosphoinositide 3-kinase (PI3K)/Akt/mTOR pathway contributes to autophagic induction following EDSV infection. Altogether, these finding lead us to conclude that EDSV infection induces autophagy, which benefits its own replication in host cells. These findings provide novel insights into EDSV-host interactions.
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Image_1_Autophagy Benefits the Replication of Egg Drop Syndrome Virus in Duck Embryo Fibroblasts.TIF
2018Co-Authors: Xueping Wang, Bo Yang, Shuying Chen, Jingyu WangAbstract:Egg Drop Syndrome virus (EDSV) is an economically important pathogen with a broad host range, and it causes disease that leads to markedly decreased Egg production. Although EDSV is known to induce apoptosis in duck embryo fibroblasts (DEFs), the interaction between EDSV and its host needs to be further researched. Here, we provide the first evidence that EDSV infection triggers autophagy in DEFs through increases in autophagosome-like double-membrane vesicles, the conversion of LC3-I to LC3-II, and LC3 colocalization with viral hexon proteins. Conversely, P62/SQSTM1 degradation, LC3-II turnover, and colocalization of LAMP and LC3 confirmed that EDSV infection triggers complete autophagy. Furthermore, we demonstrated that inhibition of autophagy by chloroquine (CQ) and 3-methyladenine (3MA) or RNA interference targeting ATG-7 decreased the yield of EDSV progeny. In contrast, induction of autophagy by rapamycin increased the EDSV progeny yield. In addition, we preliminarily demonstrated that the class I phosphoinositide 3-kinase (PI3K)/Akt/mTOR pathway contributes to autophagic induction following EDSV infection. Altogether, these finding lead us to conclude that EDSV infection induces autophagy, which benefits its own replication in host cells. These findings provide novel insights into EDSV–host interactions.
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The role of hexon in Egg Drop Syndrome virus (EDSV) inducing apoptosis in duck embryo fibroblast cells.
Research in Veterinary Science, 2017Co-Authors: Zugui Wang, Xueping Wang, Jingyu WangAbstract:Although extensive efforts have been made to understand adenovirus infection in human cells, little is known for Egg Drop Syndrome virus (EDSV) infection in the avian-derived cells. In this study, the effects of EDSV infection as well as the possible role hexon protein, the main building block of the EDSV capsid, on apoptosis induction in duck embryo fibroblast (DEF) cells was examined. Flow cytometry analysis and TUNEL assay revealed that EDSV infection induced significant apoptosis in DEF cells compared with mock infected cells. Interestingly, the increase of the apoptosis rate detected in EDSV infected DEF cells were accompanied by an increased virus load in cells in a time-dependent manner. Furthermore, a time-dependent decrease in hexon protein expression levels in hexon transfected DEF cells in parallel with a gradual decrease in TUNEL-labeling cells was also observed in the current study. In addition, caspase activity detection and western blot analysis indicates that either EDSV infection or EDSV hexon transfection both induced apoptosis of DEF cells via activating both the exogenous and the mitochondrial pathway.
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Egg Drop Syndrome virus enters duck embryonic fibroblast cells via clathrin mediated endocytosis
Virus Research, 2015Co-Authors: Jingjing Huang, Dan Tan, Yang Wang, Caihong Liu, Jingyu WangAbstract:Previous studies of Egg Drop Syndrome virus (EDSV) is restricted to serological surveys, disease diagnostics, and complete viral genome analysis. Consequently, the infection characteristics and entry routes of EDSV are poorly understood. Therefore, we aimed to explore the entry pathway of EDSV into duck embryonic fibroblast (DEF) cells as well as the infection characteristics and proliferation of EDSV in primary DEF and primary chicken embryo liver (CEL) cells. Transmission electron microscopy revealed that the virus triggered DEF cell membrane invagination as early as 10 min post-infection and that integrated endocytic vesicles formed at 20 min post-infection. The virus yield in EDSV-infected DEF cells treated with chlorpromazine (CPZ), sucrose, methyl-β-cyclodextrin (MβCD), or NH4Cl was measured by quantitative real-time PCR. Compared with the mock treatment, CPZ and sucrose greatly inhibited the production of viral progeny in a dose-dependent manner, while MβCD treatment did not result in a significant difference. Furthermore, NH4Cl had a strong inhibitory effect on the production of EDSV progeny. In addition, indirect immunofluorescence demonstrated that virus particles clustered on the surface of DEF cells treated with CPZ or sucrose. These results indicate that EDSV enters DEF cells through clathrin-mediated endocytosis followed by a pH-dependent step, which is similar to the mechanism of entry of human adenovirus types 2 and 5.
Gaiping Zhang - One of the best experts on this subject based on the ideXlab platform.
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unravelling the receptor binding property of Egg Drop Syndrome virus edsv from the crystal structure of edsv fiber head
International Journal of Biological Macromolecules, 2019Co-Authors: Yapeng Song, Qiang Wei, Yunchao Liu, Hua Feng, Yumei Chen, Yanwei Wang, Yilin Bai, Guangxu Xing, Ruiguang Deng, Gaiping ZhangAbstract:Abstract Egg Drop Syndrome virus (EDSV) is an avian adenovirus that causes markedly decrease in Egg production, and in the quality of the Eggs when it infects chickens. Until now, EDSV virus-cell interactions are poorly understood, and the cellular receptor is still unknown. In the present study, we determined the atomic structure of the fiber head of EDSV (residues 377–644) at 2.74 A resolution. Structure comparison with the (chick embryo lethal orphan) CELO long fiber head and human adenovirus fiber heads reveals that the avian adenovirus may interact with the same attachment factor in a unique fashion. Based on the previous studies of CELO virus, we assumed that the chicken coxsackievirus and adenovirus receptor (CAR) may be the attachment factor. We then demonstrate that the chicken CAR serves as a cellular attachment factor for EDSV based on three lines of evidences. Taken together, the results presented here are helpful for further exploring the pathogenesis related to the interaction between EDSV and host cells, and may be used for vaccine development and intervention strategies against EDSV infection.
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development of novel subunit vaccine based on truncated fiber protein of Egg Drop Syndrome virus and its immunogenicity in chickens
Virus Research, 2019Co-Authors: Yapeng Song, Qiang Wei, Yunchao Liu, Yilin Bai, Guangxu Xing, Ruiguang Deng, Gaiping ZhangAbstract:Abstract Egg-Drop Syndrome virus (EDSV) is an avian adenovirus that causes markedly decrease in Egg production and in the quality of the Eggs when it infects chickens. In this report, we engineered truncated fiber protein containing the entire knob domain and part of the shaft region as a vaccine candidate. The protein was obtained in the soluble fraction in Escherichia coli (E. coli), and expression level after nickel-affinity purification was 126 mg/L. By means of multiple characterization methods, it is demonstrated that the recombinant protein retains the native trimeric structure. A single inoculation with the structure-stabilized recombinant protein, even at the lowest dose of 2 μg, stimulated hemagglutination inhibition (HI) antibody responses in chickens, for at least 16 weeks. Neutralizing titers in sera from the protein immunized groups was similar to that of inactivated vaccine immunized group. The lymphocyte proliferation response and cytokine secretion were also induced in immunized SPF chickens. In addition, immunization with the fiber protein also significantly reduced the viral load in the liver. Taken together, these results suggest the truncated fiber protein as an effective single dose, long lasting and rapidly effective vaccine to protect against EDSV.
Lin Liang - One of the best experts on this subject based on the ideXlab platform.
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real time fluorescence loop mediated isothermal amplification assay for direct detection of Egg Drop Syndrome virus
BMC Veterinary Research, 2018Co-Authors: Makay Zheney, Zhambul Kaziyev, Gulmira Kassenova, Lingna Zhao, Wei Liu, Lin LiangAbstract:Egg Drop Syndrome (EDS), caused by the adenovirus “Egg Drop Syndrome virus” (EDSV) causes severe economic losses through reduced Egg production in breeder and layer flocks. The diagnosis of EDSV has been done by molecular tools since its complete genome sequence was identified. In order to enhance the capabilities of the real-time fluorescence loop-mediated isothermal amplification (RealAmp) assay, we aimed to apply the method for direct detection of the EDSV without viral DNA extraction. In order to detect the presence of the EDSV DNA, three pairs of primers were designed, from the conserved region of fiber gene of the EDSV. For our assay, test and control samples were directly used in the reaction mixture in 10-fold serial dilution. The target DNA was amplified at 65 °C, which yield positive results in a relatively short period of 40–45 min. The method reported in this study is highly sensitive as compared to polymerase chain reaction (PCR) and showed no sign of cross-reactivity or false positive results. The RealAmp accomplished specific identification of EDSV among a variety of poultry disease viruses. The direct RealAmp can be used to detect the presence of EDSV. As our result showed, the RealAmp method could be suitable for the direct detection of other DNA viruses.
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Real-time fluorescence loop-mediated isothermal amplification assay for direct detection of Egg Drop Syndrome virus
'Springer Science and Business Media LLC', 2018Co-Authors: Makay Zheney, Zhambul Kaziyev, Gulmira Kassenova, Lingna Zhao, Wei Liu, Lin LiangAbstract:Abstract Background Egg Drop Syndrome (EDS), caused by the adenovirus “Egg Drop Syndrome virus” (EDSV) causes severe economic losses through reduced Egg production in breeder and layer flocks. The diagnosis of EDSV has been done by molecular tools since its complete genome sequence was identified. In order to enhance the capabilities of the real-time fluorescence loop-mediated isothermal amplification (RealAmp) assay, we aimed to apply the method for direct detection of the EDSV without viral DNA extraction. In order to detect the presence of the EDSV DNA, three pairs of primers were designed, from the conserved region of fiber gene of the EDSV. Results For our assay, test and control samples were directly used in the reaction mixture in 10-fold serial dilution. The target DNA was amplified at 65 °C, which yield positive results in a relatively short period of 40–45 min. The method reported in this study is highly sensitive as compared to polymerase chain reaction (PCR) and showed no sign of cross-reactivity or false positive results. The RealAmp accomplished specific identification of EDSV among a variety of poultry disease viruses. Conclusions The direct RealAmp can be used to detect the presence of EDSV. As our result showed, the RealAmp method could be suitable for the direct detection of other DNA viruses
Johnson J. Rajeswar - One of the best experts on this subject based on the ideXlab platform.
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doi:10.5455/vetworld.2013.350-353 Incidence of Egg Drop Syndrome – 1976 in Namakkal district,
2013Co-Authors: P. Suresh, K. Shoba, Johnson J. RajeswarAbstract:Aim: To know the magnitude of influence by Egg Drop Syndrome – 1976 (EDS –'76) virus infection in causing Drop in Egg production in and around Namakkal. Materials and Methods: A total of 150 cloacal swabs and 15 pouch shell glands (uteri) homogenates from 15 poultry farms in and around Namakkal area were used for virus isolation. Three numbers of 10 –day- old embryonated duck Eggs were used for the inoculation of each suspected material for virus isolation. The isolate was identified by HA property, by specific inhibition of HA and by AGPT using hyperimmune serum raised against reference EDS –'76 virus strain 127. Results: Out of samples from 15 farms only one isolate (6.6%) was obtained from poultry farm No.5. Conclusion: The results of the present study revealed that the EDS –'76 virus influence in causing Drop in Egg production in this area to be minimal. Key words: cloacal swab, duck embryo, Egg Drop Syndrome, identification, isolatio
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incidence of Egg Drop Syndrome 1976 in namakkal district tamil nadu india
Veterinary World, 2013Co-Authors: P. Suresh, K. Shoba, Johnson J. RajeswarAbstract:Aim: To know the magnitude of influence by Egg Drop Syndrome – 1976 (EDS –'76) virus infection in causing Drop in Egg production in and around Namakkal. Materials and Methods: A total of 150 cloacal swabs and 15 pouch shell glands (uteri) homogenates from 15 poultry farms in and around Namakkal area were used for virus isolation. Three numbers of 10 –day- old embryonated duck Eggs were used for the inoculation of each suspected material for virus isolation. The isolate was identified by HA property, by specific inhibition of HA and by AGPT using hyperimmune serum raised against reference EDS –'76 virus strain 127. Results: Out of samples from 15 farms only one isolate (6.6%) was obtained from poultry farm No.5. Conclusion: The results of the present study revealed that the EDS –'76 virus influence in causing Drop in Egg production in this area to be minimal.
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vaccination trial for Egg Drop Syndrome 1976 eds 76
Indian Journal of Field Veterinarians, 2013Co-Authors: P. Suresh, K. Shoba, Johnson J. RajeswarAbstract:A vaccination trial for Egg Drop Syndrome - 1976 (EDS -‘76) was conducted in commercial layers. The commercial vaccine used in this study was found protective against a local isolate. The birds in group I which received a booster dose three weeks after first vaccination did not excrete any virus after challenge throughout the observation period but birds in group II which received single vaccination alone excreted the virus upto 9 days PI. There was no Egg abnormalities detected in vaccinated birds. The virus excretion in control group was present upto 15 days PI and the Egg abnormalities were noticed 25 days PI. The post mortem examination and histopathological findings were supportive of EDS -‘76 virus infection in control group.
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Incidence of Egg Drop Syndrome – 1976 in Namakkal district, Tamil Nadu, India
Veterinary World, 2013Co-Authors: P. Suresh, K. Shoba, Johnson J. RajeswarAbstract:Aim: To know the magnitude of influence by Egg Drop Syndrome – 1976 (EDS –'76) virus infection in causing Drop in Egg production in and around Namakkal. Materials and Methods: A total of 150 cloacal swabs and 15 pouch shell glands (uteri) homogenates from 15 poultry farms in and around Namakkal area were used for virus isolation. Three numbers of 10 –day- old embryonated duck Eggs were used for the inoculation of each suspected material for virus isolation. The isolate was identified by HA property, by specific inhibition of HA and by AGPT using hyperimmune serum raised against reference EDS –'76 virus strain 127. Results: Out of samples from 15 farms only one isolate (6.6%) was obtained from poultry farm No.5. Conclusion: The results of the present study revealed that the EDS –'76 virus influence in causing Drop in Egg production in this area to be minimal. [Vet World 2013; 6(6.000): 350-353
Xueping Wang - One of the best experts on this subject based on the ideXlab platform.
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rna seq analysis of duck embryo fibroblast cell gene expression during the early stage of Egg Drop Syndrome virus infection
Poultry Science, 2019Co-Authors: Xueping Wang, Baoping Yang, Shuntao Chen, Junwei WangAbstract:Egg Drop Syndrome virus (EDSV), a member of the family Adenoviridae and an economically important pathogen with a broad host range, leads to markedly decreased Egg production. However, the molecular mechanism underlying the host-EDSV interaction remains unclear. Here, we performed high-throughput RNA sequencing (RNA-Seq) to study the dynamic changes in host gene expression at 6, 12, and 24 hours post-infection in duck embryo fibroblasts (DEFs) infected with EDSV. Atotal of 441 differentially expressed genes (DEGs) were identified after EDSV infection. Gene Ontology category and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis revealed that these DEGs were associated with multiple biological functions, including signal transduction, host immunity, virus infection, cell apoptosis, cell proliferation, and pathogenicity-related and metabolic process signaling pathways. We screened and identified 12 DEGs for further examination by using qRT-PCR. The qRT-PCR and RNA-Seq results were highly consistent. This study analyzed viral infection and host immunity induced by EDSV infection from a novel perspective, and the results provide valuable information regarding the mechanisms underlying host-EDSV interactions, which will prove useful for the future development of antiviral drugs or vaccines for poultry, thus benefiting the entire poultry industry.
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autophagy benefits the replication of Egg Drop Syndrome virus in duck embryo fibroblasts
Frontiers in Microbiology, 2018Co-Authors: Xueping Wang, Bo Yang, Shuying Chen, Jingyu WangAbstract:Egg Drop Syndrome virus (EDSV) is an economically important pathogen with a broad host range, and it causes disease that leads to markedly decreased Egg production. Although EDSV is known to induce apoptosis in duck embryo fibroblasts (DEFs), the interaction between EDSV and its host needs to be further researched. Here, we provide the first evidence that EDSV infection triggers autophagy in DEFs through increases in autophagosome-like double-membrane vesicles, the conversion of LC3-I to LC3-II, and LC3 colocalization with viral hexon proteins. Conversely, P62/SQSTM1 degradation, LC3-II turnover, and colocalization of LAMP and LC3 confirmed that EDSV infection triggers complete autophagy. Furthermore, we demonstrated that inhibition of autophagy by chloroquine (CQ) and 3-methyladenine (3MA) or RNA interference targeting ATG-7 decreased the yield of EDSV progeny. In contrast, induction of autophagy by rapamycin increased the EDSV progeny yield. In addition, we preliminarily demonstrated that the class I phosphoinositide 3-kinase (PI3K)/Akt/mTOR pathway contributes to autophagic induction following EDSV infection. Altogether, these finding lead us to conclude that EDSV infection induces autophagy, which benefits its own replication in host cells. These findings provide novel insights into EDSV-host interactions.
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Image_1_Autophagy Benefits the Replication of Egg Drop Syndrome Virus in Duck Embryo Fibroblasts.TIF
2018Co-Authors: Xueping Wang, Bo Yang, Shuying Chen, Jingyu WangAbstract:Egg Drop Syndrome virus (EDSV) is an economically important pathogen with a broad host range, and it causes disease that leads to markedly decreased Egg production. Although EDSV is known to induce apoptosis in duck embryo fibroblasts (DEFs), the interaction between EDSV and its host needs to be further researched. Here, we provide the first evidence that EDSV infection triggers autophagy in DEFs through increases in autophagosome-like double-membrane vesicles, the conversion of LC3-I to LC3-II, and LC3 colocalization with viral hexon proteins. Conversely, P62/SQSTM1 degradation, LC3-II turnover, and colocalization of LAMP and LC3 confirmed that EDSV infection triggers complete autophagy. Furthermore, we demonstrated that inhibition of autophagy by chloroquine (CQ) and 3-methyladenine (3MA) or RNA interference targeting ATG-7 decreased the yield of EDSV progeny. In contrast, induction of autophagy by rapamycin increased the EDSV progeny yield. In addition, we preliminarily demonstrated that the class I phosphoinositide 3-kinase (PI3K)/Akt/mTOR pathway contributes to autophagic induction following EDSV infection. Altogether, these finding lead us to conclude that EDSV infection induces autophagy, which benefits its own replication in host cells. These findings provide novel insights into EDSV–host interactions.
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The role of hexon in Egg Drop Syndrome virus (EDSV) inducing apoptosis in duck embryo fibroblast cells.
Research in Veterinary Science, 2017Co-Authors: Zugui Wang, Xueping Wang, Jingyu WangAbstract:Although extensive efforts have been made to understand adenovirus infection in human cells, little is known for Egg Drop Syndrome virus (EDSV) infection in the avian-derived cells. In this study, the effects of EDSV infection as well as the possible role hexon protein, the main building block of the EDSV capsid, on apoptosis induction in duck embryo fibroblast (DEF) cells was examined. Flow cytometry analysis and TUNEL assay revealed that EDSV infection induced significant apoptosis in DEF cells compared with mock infected cells. Interestingly, the increase of the apoptosis rate detected in EDSV infected DEF cells were accompanied by an increased virus load in cells in a time-dependent manner. Furthermore, a time-dependent decrease in hexon protein expression levels in hexon transfected DEF cells in parallel with a gradual decrease in TUNEL-labeling cells was also observed in the current study. In addition, caspase activity detection and western blot analysis indicates that either EDSV infection or EDSV hexon transfection both induced apoptosis of DEF cells via activating both the exogenous and the mitochondrial pathway.