The Experts below are selected from a list of 270 Experts worldwide ranked by ideXlab platform

Ann-mari Svennerholm - One of the best experts on this subject based on the ideXlab platform.

  • Resistance Pattern and Molecular Characterization of Enterotoxigenic Escherichia Coli (ETEC) Strains Isolated in Bangladesh.
    PloS one, 2016
    Co-Authors: Yasmin Ara Begum, Ann-mari Svennerholm, Alaullah Sheikh, Kaisar A. Talukder, Ishrat J. Azmi, Mohammad Shahnaij, Salma Sharmin, Firdausi Qadri
    Abstract:

    Background Enterotoxigenic Escherichia Coli (ETEC) is a common cause of bacterial infection leading to acute watery diarrhea in infants and young children as well as in travellers to ETEC endemic countries. Ciprofloxacin is a broad-spectrum antimicrobial agent nowadays used for the treatment of diarrhea. This study aimed to characterize ciprofloxacin resistant ETEC strains isolated from diarrheal patients in Bangladesh.

  • Implications of Enterotoxigenic Escherichia Coli genomics for vaccine development.
    Expert review of vaccines, 2014
    Co-Authors: Åsa Sjöling, Astrid Von Mentzer, Ann-mari Svennerholm
    Abstract:

    Enterotoxigenic Escherichia Coli (ETEC) is a major cause of morbidity and mortality caused by diarrhea in children less than 5 years of age in low- and middle-income countries. Despite a wealth of research elucidating the mechanisms of disease, the immunological responses and vaccine development, ETEC is still relatively uncharacterized when it comes to regulation of virulence and detailed immune mechanisms. The recent emergence of next-generation sequencing now offers the possibility to screen genomes of ETEC strains isolated globally to identify novel vaccine targets in addition to those already established. In this review, we discuss how recent findings on ETEC genomics using novel sequencing techniques will aid in finding novel protective antigens that can be used in vaccine approaches.

  • recent progress toward an Enterotoxigenic Escherichia Coli vaccine
    Expert Review of Vaccines, 2012
    Co-Authors: Ann-mari Svennerholm, Anna Lundgren
    Abstract:

    Enterotoxigenic Escherichia Coli(ETEC) is the most common cause of bacterial diarrhea in children in Africa, Asia and Latin America and in travelers to these regions. Despite this, no effective vaccine for ETEC is available. ETEC causes disease by colonizing the small intestine with colonization factors, most of which are fimbriae, and production of heat-labile and/or heat-stable enterotoxins. Antibodies against heat-labile enterotoxin and the colonization factors have been shown to be protective, and local immunity in the gut seems to be of prime importance for protection. Hence, several inactivated and live candidate ETEC vaccines consisting of toxin antigens, alone or together with colonization factors, have been evaluated in clinical trials. In this review, the authors describe ETEC vaccine development in progress and the rationale for constructing different types of vaccines. They also discuss possibilities of enhancing immune responses to candidate ETEC vaccines, particularly in children.

  • Enterotoxigenic Escherichia Coli multilocus sequence types in Guatemala and Mexico.
    Emerging infectious diseases, 2010
    Co-Authors: Matilda Nicklasson, Ann-mari Svennerholm, John D. Klena, Claudia Rodas, A. L. Bourgeois, Olga Torres, Åsa Sjöling
    Abstract:

    The genetic backgrounds of 24 Enterotoxigenic Escherichia Coli (ETEC) strains from Mexico and Guatemala expressing heat-stable toxin (ST) and Coli surface antigen 6 (CS6) were analyzed. US travelers to these countries and resident children in Guatemala were infected by ETEC strains of sequence type 398, expressing STp and carrying genetically identical CS6 sequences.

  • Development of Multiplex PCR Assays for Detection of Enterotoxigenic Escherichia Coli Colonization Factors and Toxins
    Journal of clinical microbiology, 2009
    Co-Authors: Claudia Rodas, Ann-mari Svennerholm, Firdausi Qadri, Volga Iñiguez, Gudrun Wiklund, Åsa Sjöling
    Abstract:

    Four multiplex PCR assays for detection of 19 Enterotoxigenic Escherichia Coli (ETEC) colonization factors and an improved ETEC toxin multiplex PCR were developed and tested on Bangladeshi and Bolivian ETEC strain collections. The assays will be useful for surveillance of ETEC infections in diagnostic laboratories that have access to PCR.

Åsa Sjöling - One of the best experts on this subject based on the ideXlab platform.

  • The different ecological niches of Enterotoxigenic Escherichia Coli
    Environmental microbiology, 2015
    Co-Authors: Lucia Gonzales-siles, Åsa Sjöling
    Abstract:

    Enterotoxigenic Escherichia Coli (ETEC) is a water and food-borne pathogen that infects the small intestine of the human gut and causes diarrhoea. Enterotoxigenic E. Coli adheres to the epithelium by means of colonization factors and secretes two enterotoxins, the heat labile toxin and/or the heat stable toxin that both deregulate ion channels and cause secretory diarrhoea. Enterotoxigenic E. Coli as all E. Coli, is a versatile organism able to survive and grow in different environments. During transmission and infection, ETEC is exposed to various environmental cues that have an impact on survivability and virulence. The ability to cope with exposure to different stressful habitats is probably shaping the pool of virulent ETEC strains that cause both endemic and epidemic infections. This review will focus on the ecology of ETEC in its different habitats and interactions with other organisms as well as abiotic factors.

  • Implications of Enterotoxigenic Escherichia Coli genomics for vaccine development.
    Expert review of vaccines, 2014
    Co-Authors: Åsa Sjöling, Astrid Von Mentzer, Ann-mari Svennerholm
    Abstract:

    Enterotoxigenic Escherichia Coli (ETEC) is a major cause of morbidity and mortality caused by diarrhea in children less than 5 years of age in low- and middle-income countries. Despite a wealth of research elucidating the mechanisms of disease, the immunological responses and vaccine development, ETEC is still relatively uncharacterized when it comes to regulation of virulence and detailed immune mechanisms. The recent emergence of next-generation sequencing now offers the possibility to screen genomes of ETEC strains isolated globally to identify novel vaccine targets in addition to those already established. In this review, we discuss how recent findings on ETEC genomics using novel sequencing techniques will aid in finding novel protective antigens that can be used in vaccine approaches.

  • Enterotoxigenic Escherichia Coli multilocus sequence types in Guatemala and Mexico.
    Emerging infectious diseases, 2010
    Co-Authors: Matilda Nicklasson, Ann-mari Svennerholm, John D. Klena, Claudia Rodas, A. L. Bourgeois, Olga Torres, Åsa Sjöling
    Abstract:

    The genetic backgrounds of 24 Enterotoxigenic Escherichia Coli (ETEC) strains from Mexico and Guatemala expressing heat-stable toxin (ST) and Coli surface antigen 6 (CS6) were analyzed. US travelers to these countries and resident children in Guatemala were infected by ETEC strains of sequence type 398, expressing STp and carrying genetically identical CS6 sequences.

  • Development of Multiplex PCR Assays for Detection of Enterotoxigenic Escherichia Coli Colonization Factors and Toxins
    Journal of clinical microbiology, 2009
    Co-Authors: Claudia Rodas, Ann-mari Svennerholm, Firdausi Qadri, Volga Iñiguez, Gudrun Wiklund, Åsa Sjöling
    Abstract:

    Four multiplex PCR assays for detection of 19 Enterotoxigenic Escherichia Coli (ETEC) colonization factors and an improved ETEC toxin multiplex PCR were developed and tested on Bangladeshi and Bolivian ETEC strain collections. The assays will be useful for surveillance of ETEC infections in diagnostic laboratories that have access to PCR.

Firdausi Qadri - One of the best experts on this subject based on the ideXlab platform.

Philip R. Hardwidge - One of the best experts on this subject based on the ideXlab platform.

  • Complete Genome Sequences of Two Porcine Enterotoxigenic Escherichia Coli Strains.
    Genome announcements, 2018
    Co-Authors: Ningning Song, Philip R. Hardwidge, Siguo Liu
    Abstract:

    ABSTRACT Enterotoxigenic Escherichia Coli (ETEC) is one of the main causes of illness and death in neonatal and recently weaned pigs. Here, we sequenced the genomes of two ETEC strains that were previously used as inactivated vaccines in China.

  • Porcine aminopeptidase N binds to F4+ Enterotoxigenic Escherichia Coli fimbriae
    Veterinary Research, 2016
    Co-Authors: Pengpeng Xia, Philip R. Hardwidge, Yiting Wang, Congrui Zhu, Yajie Zou, Ying Yang, Wei Liu, Guoqiang Zhu
    Abstract:

    AbstractF4+ Enterotoxigenic Escherichia Coli (ETEC) strains cause diarrheal disease in neonatal and post-weaned piglets. Several different host receptors for F4 fimbriae have been described, with porcine aminopeptidase N (APN) reported most recently. The FaeG subunit is essential for the binding of the three F4 variants to host cells. Here we show in both yeast two-hybrid and pulldown assays that APN binds directly to FaeG, the major subunit of F4 fimbriae, from three serotypes of F4+ ETEC. Modulating APN gene expression in IPEC-J2 cells affected ETEC adherence. Antibodies raised against APN or F4 fimbriae both reduced ETEC adherence. Thus, APN mediates the attachment of F4+E. Coli to intestinal epithelial cells.

  • Draft Genome Sequence of Enterotoxigenic Escherichia Coli Strain W25K.
    Genome announcements, 2014
    Co-Authors: Wenkai Ren, Gang Liu, Jie Yin, Shuai Chen, Xiangfeng Kong, Yuanyi Peng, Yulong Yin, Philip R. Hardwidge
    Abstract:

    ABSTRACT Enterotoxigenic Escherichia Coli (ETEC) is a major cause of diarrheal disease in humans and newly weaned pigs. Here, we report the draft genome sequence of ETEC strain W25K, which causes diarrhea in piglets.

  • Enterotoxigenic Escherichia Coli infection induces intestinal epithelial cell autophagy
    Veterinary Microbiology, 2014
    Co-Authors: Yulong Tang, Gang Liu, Xiangfeng Kong, Philip R. Hardwidge, Bie Tan, Yulong Yin
    Abstract:

    The morbidity and mortality in piglets caused by Enterotoxigenic Escherichia Coli (ETEC) results in large economic losses to the swine industry, but the precise pathogenesis of ETEC-associated diseases remains unknown. Intestinal epithelial cell autophagy serves as a host defense against pathogens. We found that ETEC induced autophagy, as measured by both the increased punctae distribution of GFP-LC3 and the enhanced conversion of LC3-I to LC3-II. Inhibiting autophagy resulted in decreased survival of IPEC-1 cells infected with ETEC. ETEC triggered autophagy in IPEC-1 cells through a pathway involving the mammalian target of rapamycin (mTOR), the extracellular signal-regulated kinases 1/2 (ERK1/2), and the AMP-activated protein kinase (AMPK).

  • Molecular mechanisms of Enterotoxigenic Escherichia Coli infection.
    Microbes and infection, 2009
    Co-Authors: James M. Fleckenstein, George P. Munson, Philip R. Hardwidge, Halvor Sommerfelt, David A. Rasko, Hans Steinsland
    Abstract:

    Enterotoxigenic Escherichia Coli (ETEC) are a major cause of diarrheal illness in developing countries, and perennially the most common cause of traveller's diarrhea. ETEC constitute a diverse pathotype that elaborate heat-labile and/or heat-stable enterotoxins. Recent molecular pathogenesis studies reveal sophisticated pathogen-host interactions that might be exploited in efforts to prevent these important infections. While vaccine development for these important pathogens remains a formidable challenge, extensive efforts that attempt to exploit new genomic and proteomic technology platforms in discovery of novel targets are presently ongoing.

Piao Fan-ze - One of the best experts on this subject based on the ideXlab platform.

  • Identification of virulence factors of Enterotoxigenic Escherichia Coli strains from calves via multiplex PCR
    Chinese journal of veterinary science, 2007
    Co-Authors: Piao Fan-ze
    Abstract:

    Enterotoxigenic Escherichia Coli(ETEC) with virulence factors of K99 and/or F41 fimbriae and heat-stable enterotoxin a(STa) is a common pathogen causing diarrhea and acute enterotoxinemia in calves.Fast and reliable detection of the virulence factors is essential for identification and characterization of ETEC.A multiplex PCR method was developed to identify Enterotoxigenic Escherichia Coli strains by amplifying genes encoding K99 and F41 fimbriae,heat-stable enterotoxin.The parameters of PCR amplification,including concentration of dNTP,Mg2+,primers and annealing temperature were optimized.The specification and sensitivity of the multiplex PCR experiment were carried out on the basis of optimized parameters.It suggested that this multiplex PCR method was specific,sensitive and useful tool for identify ETEC strains from calves.