The Experts below are selected from a list of 756 Experts worldwide ranked by ideXlab platform
Yoko Aida - One of the best experts on this subject based on the ideXlab platform.
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cat1 slc7a1 acts as a cellular receptor for Bovine leukemia virus infection
The FASEB Journal, 2019Co-Authors: Lanlan Bai, Hirotaka Sato, Yoshinao Kubo, Satoshi Wada, Yoko AidaAbstract:Bovine leukemia virus (BLV) is the causative agent of Enzootic Bovine Leukosis, the most common neoplastic disease of cattle, which is closely related to human T-cell leukemia viruses. BLV has spre...
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blv cocomo qpcr 2 improvements to the blv cocomo qpcr assay for Bovine leukemia virus by reducing primer degeneracy and constructing an optimal standard curve
Archives of Virology, 2015Co-Authors: Shinnosuke Takeshima, Yuri Kitamuramuramatsu, Yuan Yuan, Meripet Polat, Susumu Saito, Yoko AidaAbstract:Bovine leukemia virus (BLV) is the etiological agent of Enzootic Bovine Leukosis, which is the most common neoplastic disease of cattle. Because BLV infection can remain clinically silent, the proviral load is an important index for estimating disease progression. CoCoMo-qPCR, an assay developed to estimate BLV proviral load, allows the highly sensitive detection of BLV originating in different countries. Here, we developed a modified version of the CoCoMo-qPCR assay, the “BLV-CoCoMo-qPCR-2” assay, which uses optimized degenerate primers. We also constructed a new plasmid standard. Finally, we used both assays to examine DNA samples from BLV-infected cattle and compared the results.
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identification of Bovine leukemia virus tax function associated with host cell transcription signaling stress response and immune response pathway by microarray based gene expression analysis
BMC Genomics, 2012Co-Authors: Mariluz Arainga, Eri Takeda, Yoko AidaAbstract:Background Bovine leukemia virus (BLV) is associated with Enzootic Bovine Leukosis and is closely related to human T-cell leukemia virus type I. The Tax protein of BLV is a transcriptional activator of viral replication and a key contributor to oncogenic potential. We previously identified interesting mutant forms of Tax with elevated (TaxD247G) or reduced (TaxS240P) transactivation effects on BLV replication and propagation. However, the effects of these mutations on functions other than transcriptional activation are unknown. In this study, to identify genes that play a role in the cascade of signal events regulated by wild-type and mutant Tax proteins, we used a large-scale host cell gene-profiling approach.
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blv cocomo qpcr quantitation of Bovine leukemia virus proviral load using the cocomo algorithm
Retrovirology, 2010Co-Authors: Mayuko Jimba, Shinnosuke Takeshima, Kazuhiro Matoba, Daiji Endoh, Yoko AidaAbstract:Background Bovine leukemia virus (BLV) is closely related to human T-cell leukemia virus (HTLV) and is the etiological agent of Enzootic Bovine Leukosis, a disease characterized by a highly extended course that often involves persistent lymphocytosis and culminates in B-cell lymphomas. BLV provirus remains integrated in cellular genomes, even in the absence of detectable BLV antibodies. Therefore, to understand the mechanism of BLV-induced leukemogenesis and carry out the selection of BLV-infected animals, a detailed evaluation of changes in proviral load throughout the course of disease in BLV-infected cattle is required. The aim of this study was to develop a new quantitative real-time polymerase chain reaction (PCR) method using Coordination of Common Motifs (CoCoMo) primers to measure the proviral load of known and novel BLV variants in clinical animals.
Hisashi Inokuma - One of the best experts on this subject based on the ideXlab platform.
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analysis of the bone morphogenetic protein 6 gene promoter region in young beef cattle affected by Enzootic Bovine Leukosis
Journal of Veterinary Medical Science, 2021Co-Authors: Masaki Maezawa, Yoshiyasu Kobayashi, Kotaro Matsumoto, Kenichi Watanabe, Haruko Ogawa, Hisashi InokumaAbstract:Enzootic Bovine Leukosis (EBL) is typically observed in cattle over 3 years old. However, some cases of EBL onset in young beef cattle have been reported in Japan. The mechanism for early EBL onset is unclear. In Japan, beef cattle are given large amounts of concentrated feed with low vitamin A. Bone morphogenetic proteins (BMPs) are regulators of cell proliferation, differentiation, and apoptosis, and thought to represent one of the key players in tumor malignancy. The purpose of this study was to evaluate the differences in BMP-6 methylation status between EBL beef cattle under 3 years old and other cattle. We investigated the methylation status of the BMP-6 promoter region in 32 EBL beef cattle under 3 years old. We also compared the methylation status of EBL dairy cattle to that of healthy cattle. Median methylation rate of the BMP-6 promoter region in EBL beef cattle under 3 years old was 8.9%, which was significantly higher than that of other groups. Hypermethylation of the BMP-6 promoter region might contribute to early onset of EBL in beef cattle under 3 years old, and animal feeding management practices specific to beef cattle may affect the methylation status of the BMP-6 promoter region.
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analysis of Bovine leukemia virus integration sites in cattle under 3 years old with Enzootic Bovine Leukosis
Archives of Virology, 2020Co-Authors: Masaki Maezawa, Hisashi InokumaAbstract:In the present study, we analyzed Bovine leukemia virus (BLV) integration sites in under 3 years old with Enzootic Bovine Leukosis (EBL) cattle and compared these to 30 cattle over 3 years old with EBL. BLV proviruses were integrated near CpG islands and into long interspersed nuclear elements more frequently in EBL cattle under 3 years old than in those over 3 years old. These results suggest that cattle under 3 years old with EBL have different BLV provirus integration sites from those of cattle over 3 years old with EBL, and the BLV provirus integration site may represent one factor contributing to early onset of EBL.
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detection of monoclonal integration of Bovine leukemia virus proviral dna as a malignant marker in two Enzootic Bovine Leukosis cases with difficult clinical diagnosis
Journal of Veterinary Medical Science, 2015Co-Authors: Saori Miura, Noriyuki Horiuchi, Yoshiyasu Kobayashi, Kotaro Matsumoto, Shinichiro Kawazu, Hisashi InokumaAbstract:Monoclonal integration of Bovine leukemia virus (BLV) proviral DNA into Bovine genomes was detected in peripheral blood from two clinical cases of Enzootic Bovine Leukosis (EBL) without enlargement of superficial lymph nodes. A BLV-specific probe hybridized with 1 to 3 EcoRI and HindIII fragments in these 2 atypical EBL cattle by Southern blotting and hybridization, as well as in 3 typical EBL cattle. The probe also hybridized to a large number of EcoRI and HindIII fragments in 5 cattle with persistent Leukosis. These results suggest that the detection of monoclonal integration of BLV provirus into the host genome may serve as a marker of monoclonal proliferation and malignancy in difficult to diagnose EBL cattle.
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NOTE Internal Medicine
2015Co-Authors: Hisashi InokumaAbstract:Detection of monoclonal integration of Bovine leukemia virus proviral DNA as a malignant marker in two Enzootic Bovine Leukosis cases with difficult clinical diagnosi
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Evaluation of gene expression in peripheral blood cells as a potential biomarker for Enzootic Bovine Leukosis.
The Journal of veterinary medical science, 2013Co-Authors: Mohammad Monir Tawfeeq, Hidefumi Furuoka, Noriyuki Horiuchi, Yoshiyasu Kobayashi, Hisashi InokumaAbstract:Expression of six selective genes in peripheral blood cells was evaluated as diagnostic biomarkers for Enzootic Bovine Leukosis (EBL) by using 10 EBL and 15 clinically healthy cattle. The clinically healthy cattle generally showed lower gene expression levels. Although wide variations of gene expression were found in some clinical cases of EBL, 4 and 5 among 10 EBL cattle showed higher expression of interleukin 2 receptor gene (IL2R) and Wilms' tumor gene (WT1), respectively. Expression of IL2R in peripheral blood cells in EBL cattle was statistically increased; however, the lower sensitivity and higher variation in the gene expressions among clinical cases of EBL would be problems as diagnostic biomarkers.
Kenji Murakami - One of the best experts on this subject based on the ideXlab platform.
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comparison of the copy numbers of Bovine leukemia virus in the lymph nodes of cattle with Enzootic Bovine Leukosis and cattle with latent infection
Archives of Virology, 2014Co-Authors: Yoshiko Somura, Kenji Murakami, Emi Sugiyama, Hiroshi FujikawaAbstract:To establish a diagnostic index for predicting Enzootic Bovine Leukosis (EBL), proviral Bovine leukemia virus (BLV) copies in whole blood, lymph nodes and spleen were examined by quantitative real-time PCR (qPCR). Cattle were divided into two groups, EBL and BLV-infected, based on meat inspection data. The number of BLV copies in all specimens of EBL cattle was significantly higher than those of BLV-infected cattle (p < 0.0001), and the number of BLV copies in the lymph nodes was particularly large. Over 70 % of the superficial cervical, medial iliac and jejunal lymph nodes from EBL cattle had more than 1,000 copies/10 ng DNA, whereas lymph nodes from BLV-infected cattle did not. These findings suggest that the cattle harboring more than 1,000 BLV copies may be diagnosed with EBL.
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risk factors associated with within herd transmission of Bovine leukemia virus on dairy farms in japan
BMC Veterinary Research, 2010Co-Authors: Sota Kobayashi, Misako Konishi, Toshiyuki Tsutsui, Kenichiro Kameyama, Takehisa Yamamoto, Yoko Hayama, Kenji MurakamiAbstract:Background Although several attempts have been made to control Enzootic Bovine Leukosis (EBL) at the local level, a nationwide control program has not been implemented in Japan, except for passive surveillance. Effective control of EBL requires that the transmission routes of Bovine leukemia virus (BLV) infection should be identified and intercepted based on scientific evidence. In this cross-sectional study, we examined the risk factors associated with within-herd transmission of BLV on infected dairy farms in Japan. Blood samples taken from 30 randomly selected adult cows at each of 139 dairy farms were tested by enzyme-linked immunosorbent assay (ELISA). Information on herd management was collected using a structured questionnaire.
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b 1a b 1b and conventional b cell lymphoma from Enzootic Bovine Leukosis
Veterinary Immunology and Immunopathology, 1996Co-Authors: Kazuhito Takahashi, Masanobu Goryo, M Asahina, Y Aida, Kenji Murakami, Kenji Tani, Atsushi Koguchi, Kosuke OkadaAbstract:In order to characterize the phenotypes of tumor cells and to clarify from which B cell lineage the lymphomas were derived, ten cows with Enzootic Bovine Leukosis were examined by means of immunohistologic staining and flow cytometry. The tumor cells expressed mainly major histocompatibility complex (MHC) class II+ (10/10), BoCD11b+ (9/10), IgG1+ (8/10), B-B2+ (8/10) BoCD5+ (7/10), and lambda light chain+ (7/10). Tumor cells from only one animal expressed sIgM+ (1/10). Tumor cells from all ten animals were negative for IgG2, BoCD3, BoCD4, BoCD8, WC1-N2, and IL-2R alpha. The phenotypes of these tumor cells were all slightly different, suggesting that Bovine leukemia virus (BLV)-induced lymphoma expresses phenotypic diversity. Moreover, tumor cells from seven cattle coexpressed BoCD5 and BoCD11b (B-1a cells). On the other hand, tumor cells from two of them only expressed BoCD11b (B-1b cells), and those from one were negative for both BoCD5 and BoCD11b (conventional B cells). Therefore, we concluded that BLV-induced lymphoma cells can be derived from B-1a, B-1b and conventional B cells.
Asami Nishimori - One of the best experts on this subject based on the ideXlab platform.
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Establishment of a simplified inverse polymerase chain reaction method for diagnosis of Enzootic Bovine Leukosis
Archives of Virology, 2021Co-Authors: Asami Nishimori, Kiyohiko Andoh, Yuichi Matsuura, Asuka Kumagai, Shinichi HatamaAbstract:Enzootic Bovine Leukosis (EBL) is a malignant B-cell lymphoma of cattle caused by infection with Bovine leukemia virus (BLV). It is defined by clonal and neoplastic expansion of BLV-infected B cells. Currently, multiple examinations are able to comprehensively diagnose this condition. Inverse polymerase chain reaction (PCR) is a useful method to determine retrovirus integration sites. Here, we established a simplified inverse PCR method, involving the evaluation of clonality and similarity of BLV integration sites, to clinically diagnose EBL, and we also assessed its reliability. We found that the novel BLV inverse PCR could detect clonal expansion of infected cells even if they constituted only 5% of the total number of cells, while not amplifying any fragments from BLV-uninfected cells, thus confirming its sufficient sensitivity and specificity for use in EBL diagnosis. Furthermore, 50 clinical cases of Bovine leukemia were analyzed using BLV inverse PCR and other PCR-based methods, wherein our method most efficiently determined virus-dependent Bovine leukemia, including unidentified clinical cases observed in a previous report. Following further clinical investigations to enhance its reliability, the proposed BLV inverse PCR method has the potential to be applied to EBL diagnosis.
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identification of an atypical Enzootic Bovine Leukosis in japan by using a novel classification of Bovine leukemia based on immunophenotypic analysis
Clinical and Vaccine Immunology, 2017Co-Authors: Asami Nishimori, Satoru Konnai, Yuzumi Chiba, Tomohiro Okagawa, Naoya Maekawa, Shinya Goto, Ryoyo Ikebuchi, Ayako Nakahara, Masaho Ikeda, Shiro MurataAbstract:Bovine leukemia is classified into two types: Enzootic Bovine Leukosis (EBL) and sporadic Bovine Leukosis (SBL). EBL is caused by infection with Bovine leukemia virus (BLV), which induces persistent lymphocytosis and B-cell lymphoma in cattle after a long latent period. Although it has been demonstrated that BLV-associated lymphoma occurs predominantly in adult cattle of >3 to 5 years, suspicious cases of EBL onset in juvenile cattle were recently reported in Japan. To investigate the current status of Bovine leukemia in Japan, we performed immunophenotypic analysis of samples from 50 cattle that were clinically diagnosed as having Bovine leukemia. We classified the samples into five groups on the basis of the analysis and found two different types of EBL: classic EBL (cEBL), which has the familiar phenotype commonly known as EBL, and polyclonal EBL (pEBL), which exhibited neoplastic proliferation of polyclonal B cells. Moreover, there were several atypical EBL cases even in cEBL, including an early onset of EBL in juvenile cattle. A comparison of the cell marker expressions among cEBL, pEBL, and B-cell-type SBL (B-SBL) revealed characteristic patterns in B-cell leukemia, and these patterns could be clearly differentiated from those of healthy phenotypes, whereas it was difficult to discriminate between cEBL, pEBL, and B-SBL only by the expression patterns of cell markers. This study identified novel characteristics of Bovine leukemia that should contribute to a better understanding of the mechanism underlying tumor development in BLV infection.
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identification of an atypical Enzootic Bovine Leukosis in japan by using a novel classification of Bovine leukemia based on immunophenotypic analysis
Clinical and Vaccine Immunology, 2017Co-Authors: Asami Nishimori, Satoru Konnai, Yuzumi Chiba, Tomohiro Okagawa, Naoya Maekawa, Shinya Goto, Ryoyo Ikebuchi, Ayako Nakahara, Masaho Ikeda, Shiro MurataAbstract:Bovine leukemia is classified into two types: Enzootic Bovine Leukosis (EBL) and sporadic Bovine Leukosis (SBL). EBL is caused by infection with Bovine leukemia virus (BLV) which induces persistent lymphocytosis and B-cell lymphoma in cattle after a long latent period. Although it has been demonstrated that BLV-associated lymphoma occurs predominantly in adult cattle >3--5 years, suspicious cases of EBL onset in juvenile cattle were recently reported in Japan. To investigate the current status of Bovine leukemia in Japan, we performed immunophenotypic analysis of samples from 50 cattle that were clinically diagnosed as having Bovine leukemia. We classified the samples into five groups on the basis of the analysis and found two different types of EBL: classic EBL (cEBL) was the familiar phenotype commonly known as EBL, and polyclonal EBL (pEBL) exhibited neoplastic proliferation of polyclonal B cells. Moreover, there were several atypical EBL cases even in cEBL, including early onset of EBL in juvenile cattle. Comparison of the cell-marker expressions among cEBl, pEBL, and B-cell-type SBL (B-SBL) revealed characteristic patterns of the expression in B-cell leukemia, and these patterns could be clearly differentiated from those of healthy phenotypes, whereas it was difficult to discriminate between cEBl, pEBL, and B-SBL only by expression patterns of cell markers. This study identified novel characteristics of Bovine leukemia that should contribute to a better understanding of the mechanism underlying tumor development in BLV infection.
Shiro Murata - One of the best experts on this subject based on the ideXlab platform.
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identification of an atypical Enzootic Bovine Leukosis in japan by using a novel classification of Bovine leukemia based on immunophenotypic analysis
Clinical and Vaccine Immunology, 2017Co-Authors: Asami Nishimori, Satoru Konnai, Yuzumi Chiba, Tomohiro Okagawa, Naoya Maekawa, Shinya Goto, Ryoyo Ikebuchi, Ayako Nakahara, Masaho Ikeda, Shiro MurataAbstract:Bovine leukemia is classified into two types: Enzootic Bovine Leukosis (EBL) and sporadic Bovine Leukosis (SBL). EBL is caused by infection with Bovine leukemia virus (BLV), which induces persistent lymphocytosis and B-cell lymphoma in cattle after a long latent period. Although it has been demonstrated that BLV-associated lymphoma occurs predominantly in adult cattle of >3 to 5 years, suspicious cases of EBL onset in juvenile cattle were recently reported in Japan. To investigate the current status of Bovine leukemia in Japan, we performed immunophenotypic analysis of samples from 50 cattle that were clinically diagnosed as having Bovine leukemia. We classified the samples into five groups on the basis of the analysis and found two different types of EBL: classic EBL (cEBL), which has the familiar phenotype commonly known as EBL, and polyclonal EBL (pEBL), which exhibited neoplastic proliferation of polyclonal B cells. Moreover, there were several atypical EBL cases even in cEBL, including an early onset of EBL in juvenile cattle. A comparison of the cell marker expressions among cEBL, pEBL, and B-cell-type SBL (B-SBL) revealed characteristic patterns in B-cell leukemia, and these patterns could be clearly differentiated from those of healthy phenotypes, whereas it was difficult to discriminate between cEBL, pEBL, and B-SBL only by the expression patterns of cell markers. This study identified novel characteristics of Bovine leukemia that should contribute to a better understanding of the mechanism underlying tumor development in BLV infection.
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identification of an atypical Enzootic Bovine Leukosis in japan by using a novel classification of Bovine leukemia based on immunophenotypic analysis
Clinical and Vaccine Immunology, 2017Co-Authors: Asami Nishimori, Satoru Konnai, Yuzumi Chiba, Tomohiro Okagawa, Naoya Maekawa, Shinya Goto, Ryoyo Ikebuchi, Ayako Nakahara, Masaho Ikeda, Shiro MurataAbstract:Bovine leukemia is classified into two types: Enzootic Bovine Leukosis (EBL) and sporadic Bovine Leukosis (SBL). EBL is caused by infection with Bovine leukemia virus (BLV) which induces persistent lymphocytosis and B-cell lymphoma in cattle after a long latent period. Although it has been demonstrated that BLV-associated lymphoma occurs predominantly in adult cattle >3--5 years, suspicious cases of EBL onset in juvenile cattle were recently reported in Japan. To investigate the current status of Bovine leukemia in Japan, we performed immunophenotypic analysis of samples from 50 cattle that were clinically diagnosed as having Bovine leukemia. We classified the samples into five groups on the basis of the analysis and found two different types of EBL: classic EBL (cEBL) was the familiar phenotype commonly known as EBL, and polyclonal EBL (pEBL) exhibited neoplastic proliferation of polyclonal B cells. Moreover, there were several atypical EBL cases even in cEBL, including early onset of EBL in juvenile cattle. Comparison of the cell-marker expressions among cEBl, pEBL, and B-cell-type SBL (B-SBL) revealed characteristic patterns of the expression in B-cell leukemia, and these patterns could be clearly differentiated from those of healthy phenotypes, whereas it was difficult to discriminate between cEBl, pEBL, and B-SBL only by expression patterns of cell markers. This study identified novel characteristics of Bovine leukemia that should contribute to a better understanding of the mechanism underlying tumor development in BLV infection.