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Kit-lam Chan - One of the best experts on this subject based on the ideXlab platform.
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antiadipogenic effects of a standardized quassinoids enriched fraction and Eurycomanone from Eurycoma longifolia
Phytotherapy Research, 2018Co-Authors: D Balan, Kit-lam Chan, Dharmani Devi Murugan, Sazaly Abubakar, Pooifong WongAbstract:Bioactive compounds of Eurycoma longifolia (EL) jack were previously shown to reduce omentum fat mass and oestradiol-induced fatty uterine adhesion in rats. However, the exact role of EL on adipogenesis remains unknown. This study sought to investigate the effects of an EL standardized quassinoids-enriched fraction (SQEL) and the pure compound, Eurycomanone, on adipogenesis in 3T3-L1 preadipocyte cells. 3T3-L1 cells were induced to differentiate and treated for 8 days. The treatment reduced intracellular accumulation of lipid droplets and triglycerides in the differentiating adipocytes and induced lipolysis in matured adipocytes. The expressions of adipogenic transcription factors and markers were also significantly downregulated during the early stage of differentiation. Furthermore, SQEL also suppressed body weight gain, decreased epididymal and perirenal fat pad mass and size, and reduced the accumulation of fat in the livers of C57BL/6J mice fed with normal or high-fat diet that were concurrently given 5 mg/kg and 10 mg/kg (i.p) of SQEL for 12 weeks. SQEL also improved glucose intolerance and decreased the elevated total cholesterol and triglyceride levels in these mice groups. These findings suggest that SQEL could be explored as an alternative pharmacologic agent inhibiting adipogenesis for the prevention of obesity.
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Effects of Eurycoma longifolia standardized extract on estradiol valerate-induced teratogenicity in female rats
'WorldAcademics BOSON Academic Publishers', 2018Co-Authors: Abdul Rahman Suzanah, Ahmad, Nur Amalina, Kit-lam ChanAbstract:Estradiol valerate, a synthetic steroidal estradiol is used to treat female reproductive disorders as well as contraception. Consumption of estradiol valerate may produce abortional and teratogenic effects. Possible effects of the standardized extract of Eurycoma longifolia, TAF 273, on foetal development in female rats exposed to estradiol valerate were therefore investigated. Pregnant female rats on day 12 of gestation were administered with a single subcutaneous injection of estradiol valerate (0.15 mg/kg). TAF 273 was administered orally at doses of 25, 50 and 100 mg/kg body weight from day 12 to day 19 of gestation followed by termination of pregnancy on day 21. A decreased the number of live foetuses with high number of post-implantation loss and early resorption were observed. Administration of 100 mg/kg TAF 273 resulted in high number of live foetuses but with decreased body weight and crown-rump length possibly due to competition for space for embryonic development and blood supply. Kyphosis, scoliosis and kinky tail following treatment with estradiol valerate were apparent. Eurycoma longifolia may have potential in ameliorating teratogenic effects of estradiol valerate by reducing external abnormalities in the rat foetuses and may have prospects in the treatment of female-linked disorders and complications
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comparative x ray and conformational analysis of a new crystal of 13α 21 dihydroEurycomanone with Eurycomanone from Eurycoma longifolia and their anti estrogenic activity using the uterotrophic assay
Planta Medica, 2011Co-Authors: Chinhoe Teh, Motoo Shiro, Hiroshi Morita, Mahfoudh Abdulghani, Abas Hj Hussin, Kit-lam ChanAbstract:13 α,21-DihydroEurycomanone (1), a known quassinoid of Eurycoma longifolia Jack was recrystallized from chloroform into a novel crystal structure in space group P2 (1). Its X-ray data were compared with those of Eurycomanone ( 2). Following intraperioneal injections at similar doses of 2.44 µmol/kg/day for 3 consecutive days, 2 displayed comparable potency with tamoxifen but was more potent than 1 in the anti-estrogenic effect against 17 α-ethynylestradiol (EE)-induced uterotrophy of immature rats.
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2 3 dehydro 4α hydroxylongilactone a novel quassinoid and two known phenyl propanoids from Eurycoma longifolia jack
Food Chemistry, 2010Co-Authors: Chinhoe Teh, Hiroshi Morita, Osamu Shirota, Kit-lam ChanAbstract:Abstract A phytochemical study on the root of Eurycoma longifolia Jack afforded a novel 2,3-dehydro-4α-hydroxylongilactone (1) and two known phenyl propanoids, 2,3-dihydroxy-1-(4′-hydroxy-3′-methoxyphenyl)-propan-1-one (2) and scopolin (3), isolated from this plant for the first time. All structures were characterised by spectroscopic analysis (1D and 2D NMR experiments, HRMS, UV, IR and optical rotation) and by comparison with known data. This is the first report of a C 19 quassinoid from the Simaroubaceae family possessing an unsubstituted vinyl function and a C-4 methyl group of β-configuration in ring A.
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activity of Eurycoma longifolia root extract against plasmodium falciparum in vitro
Wiener Klinische Wochenschrift, 2009Co-Authors: Walther H Wernsdorfer, Kit-lam Chan, Sabariah Ismail, Kanungnit Congpuong, Gunther WernsdorferAbstract:The habitats of Eurycoma longifolia Jack, a slender tree, are jungles in Malaysia and Indonesia. It belongs to the family Simaroubaceae and is a source of quassinoids with anabolic, antimalarial and cytostatic activity. In this study, conducted during 2008 in Mae Sot, Thailand, a standardized extract of E. longifolia containing three major quassinoids, Eurycomanone (1), 13,21-dihydroEurycomanone (2) and 13alpha(21)-epoxyEurycomanone (3) was evaluated for antiplasmodial activity against Plasmodium falciparum and its activity has been compared with that of artemisinin, using 38 fresh parasite isolates and assessment of inhibition of schizont maturation. The IC(50), IC(90) and IC(99) values for artemisinin were 4.30, 45.48 and 310.97 microg/l, and those for the root extract from E. longifolia 14.72, 139.65 and 874.15 microg/l respectively. The GMCOC for artemisinin was 337.81 mug/l, and for the plant extract it was 807.41 microg/l. The log-concentration probit regressions were parallel. The inhibitory activity of the E. longifolia extract was higher than that expected from the three quassinoids isolated from the plant, suggesting synergism between the quassinoids or the presence of other unidentified compounds.
Chee Yan Choo - One of the best experts on this subject based on the ideXlab platform.
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Eurycomanone and Eurycomanol from Eurycoma longifolia jack as regulators of signaling pathways involved in proliferation cell death and inflammation
Molecules, 2014Co-Authors: Sheherazade Hajjouli, Chee Yan Choo, Sebastien Chateauvieux, Mariehelene Teiten, Barbora Orlikova, Marc Schumacher, Mario Dicato, Marc DiederichAbstract:Eurycomanone and Eurycomanol are two quassinoids from the roots of Eurycoma longifolia Jack. The aim of this study was to assess the bioactivity of these compounds in Jurkat and K562 human leukemia cell models compared to peripheral blood mononuclear cells from healthy donors. Both Eurycomanone and Eurycomanol inhibited Jurkat and K562 cell viability and proliferation without affecting healthy cells. Interestingly, Eurycomanone inhibited NF-κB signaling through inhibition of IκBα phosphorylation and upstream mitogen activated protein kinase (MAPK) signaling, but not Eurycomanol. In conclusion, both quassinoids present differential toxicity towards leukemia cells, and the presence of the α,β-unsaturated ketone in Eurycomanone could be prerequisite for the NF-κB inhibition.
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antiplasmodial studies of Eurycoma longifolia jack using the lactate dehydrogenase assay of plasmodium falciparum
Journal of Ethnopharmacology, 2004Co-Authors: Kit-lam Chan, Chee Yan Choo, Noor Rain Abdullah, Zakiah IsmailAbstract:The roots of Eurycoma longifolia Jack have been used as traditional medicine to treat malaria. A systematic bioactivity-guided fractionation of this plant was conducted involving the determination of the effect of its various extracts and their chemical constituents on the lactate dehydrogenase activity of in vitro chloroquine-resistant Gombak A isolate and chloroquine-sensitive D10 strain of Plasmodium falciparum parasites. Their antiplasmodial activity was also compared with their known in vitro cytotoxicity against KB cells. Four quassinoids, Eurycomanone (1), 13,21-dihydroEurycomanone (3), 13α(21)-epoxyEurycomanone (4), Eurycomalactone (6) and an alkaloid, 9-methoxycanthin-6-one (7), displayed higher antiplasmodial activity against Gombak A isolate but were less active against the D10 strain when compared with chloroquine. Amongst the compounds tested, 1 and 3 showed higher selectivity indices obtained for the cytotoxicity to antiplasmodial activity ratio than 14,15β-dihydroxyklaineanone (2), Eurycomanol (5), 6 and 7.
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the toxicity of some quassinoids from Eurycoma longifolia
Planta Medica, 2002Co-Authors: Kit-lam Chan, Chee Yan ChooAbstract:The 50 % aqueous ethanol extract of Eurycoma longifolia Jack (Simaroubaceace) roots was partitioned with diethyl ether, n-butanol and then water. Acute toxicity studies of each fraction on mice administered orally and brine shrimps revealed that the n-butanol fraction was the most toxic. Toxicity-guided chromatographic fractionation of the n-butanol fraction identified Eurycomanone as the most toxic component. 13,21-DihydroEurycoma-none, Eurycomanol, longilactone, 14,15 beta-dihydroxyklaineanone and Eurycomanol-2- O-beta-glucopyranoside were 2.8, 33, 44, 88.9 and > 100 times less toxic on brine shrimps, respectively. A C 20 -type quassinoid, an alpha,beta-unsaturated ketone in ring A, an exomethylene function at C-13 and an oxymethylene bridge connecting C-8 and C-11 of ring C contributed to increased toxicity.
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high performance liquid chromatography analysis of canthinone alkaloids from Eurycoma longifolia
Planta Medica, 2002Co-Authors: Chee Yan Choo, Kit-lam ChanAbstract:Abstract A reversed phase-high performance liquid chromatography method with a photodiode array detector was developed for the simultaneous determination of three major alkaloids, 9-methoxycanthin-6-one (1), 3-methylcanthin-5,6-dione (2) and its 9-methoxy analogue (3) in Eurycoma longifolia Jack. These alkaloids were easily separated by a gradient elution protocol of 20 - 42 % acetonitrile in 0.1 % acetic acid. Compound 1 showed characteristic absorption at 350 nm only whereas its dione analogues, 2 and 3 displayed strong absorptions at both 350 and 451 nm. The linear calibration ranges were 0.7 - 50 microg x mL(-1) for 1, 1.5-50 microg x mL(-1) for 2 and 3.1 -100 microg x mL(-1) for 3. The recoveries of the three alkaloids were 90.8-101.0% with relative standard deviations from 0.35 to 6.31 % (n = 3). The limits of detection for all the alkaloids were within the range of 0.35 - 0.7 microg x mL(-1). This method was successfully applied to the phytochemical analysis of E. longifolia roots obtained from different sources.
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high performance liquid chromatography in phytochemical analysis of Eurycoma longifolia
Planta Medica, 1998Co-Authors: Kit-lam Chan, Hiroshi Morita, Chee Yan Choo, Hideji ItokawaAbstract:An analytical method using HPLC with UV detection was developed to investigate the quassinoid content of Eurycoma longifolia Jack (Simaroubaceae) collected from various sources. Eurycomanone (1), longilactone (2), 14,15beta-dihydroxyklaineanone (3), 15beta-acetyl-14-hydroxyklaineanone (4), 6alpha-hydroxyEurycomalactone (5), and Eurycomalactone (7) were isolated as reference standards and together with the synthesized 1beta,12alpha,15beta-triacetylEurycomanone (6, internal standard), were identified by NMR, MS, UV and IR spectroscopies. Their coefficient of variation values for 0.50-35 microg ml(-1) concentrations of quassinoids and their retention times measured within- and between-day were small. The recoveries of the spiked quassinoids in E. longifolia samples and their detection limits at 8.5 times signal to noise ratio were 99.75-109.13% and 0.01 microg ml(-1), respectively. From the root samples analysed, 1 had the highest concentration, being about 16.8-39.6 fold higher than the other quassinoids 2, 3, 5, 7 but 145.3 fold higher than 4 which showed the lowest concentration.
Hooi Hoon Ang - One of the best experts on this subject based on the ideXlab platform.
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sexual arousal in sexually sluggish old male rats after oral administration of Eurycoma longifolia jack
Journal of basic and clinical physiology and pharmacology, 2004Co-Authors: Hooi Hoon Ang, Kheng Leng Lee, Matsumoto KiyoshiAbstract:Eurycoma longifolia Jack commonly known as Tongkat Ali in Malaysia, has been used in Malaysia to increase male virility and sexual prowess. The objective of this study is to evaluate sexual arousal in sexually sluggish old male rats, 24 months old and retired breeders, receiving 200, 400, or 800 mg/kg of various fractions of E. longifolia Jack, twice daily, for 10 days. Control rats received 3 ml/kg of normal saline. The aphrodisiac effect was monitored by the act of yawning and stretching because yawning, either alone or associated with stretching, is considered an ancestral vestige surviving throughout evolution that promotes sexual arousal. The results showed that 800 mg/kg of E. longifolia Jack increased yawning by 50% and stretching by 16.7% in sexually sluggish old male rats, by 676-719% and 31-336%, respectively, in sexually active male rats, and by 22-44% and 75-100%, respectively, in middle aged, 9 months old and retired breeders. We conclude that the results of this study support the folk use of this plant as an aphrodisiac.
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Eurycoma longifolia jack enhances sexual motivation in middle aged male mice
Journal of basic and clinical physiology and pharmacology, 2003Co-Authors: Hooi Hoon Ang, Kheng Leng Lee, Matsumoto KiyoshiAbstract:Eurycoma longifolia Jack was investigated for sexual motivation activity in adult, middle-aged male mice and in retired breeders, using the modified open field and the modified runway choice methods. Each mouse received 500 mg/kg of one of 4 fractions of E. longifolia Jack, viz. chloroform, methanol, butanol, and water, whereas the mice in the control and yohimbine groups received 3 ml/kg of normal saline and 30 mg/kg of yohimbine daily respectively for 10 d. The results show a transient increase in the percentage of male mice responding to the right choice after chronic consumption of the fractions with 50 percent of the adult middle-aged male mice treated with E. longifolia Jack and yohimbine scoring the right choice after 8 and 5 days post-treatment respectively. In conclusion, this study has shown that E. longifolia Jack continues to enhance sexual motivation in adult, middle-aged male mice and in retired breeders.
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Quassinoids from Eurycoma longifolia
Phytochemistry, 2002Co-Authors: Hooi Hoon Ang, Yukio Hitotsuyanagi, Haruhiko Fukaya, Koichi TakeyaAbstract:Three quassinoids, eurycolactone D (1), eurycolactone E (2) and eurycolactone F (3) were isolated from the roots of Eurycoma longifolia Jack. The structures of 1-3 were elucidated by spectroscopic methods, and that of 3 was further confirmed by X-ray crystallography. The known quassinoids, laurycolactone B (4) and Eurycomalactone (5) were also identified.
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effect of Eurycoma longifolia jack on libido in middle aged male rats
Journal of basic and clinical physiology and pharmacology, 2002Co-Authors: Hooi Hoon Ang, Kheng Leng LeeAbstract:The effect of increasing doses of various fractions of Eurycoma longifolia Jack extracts on libido was examined in middle-aged male rats. The results showed that a high dose (800 mg/kg) of all E. longifolia Jack extracts significantly increased mount frequency (MF) (P < 0.05) over that of untreated controls, but had no effect on the frequency of intromission or ejaculation. Methanol, chloroform, water, and butanol fractions exhibited MF of 2.5 +/- 0.1, 2.6 +/- 0.3, 2.5 +/- 0.1 and 2.6 +/- 0.2, respectively, in adult, middle-aged male rats, and retired breeders versus 2.3 +/- 0.1 in untreated controls. This translated to a minor increase in MF of 8.7%, 13.0%, 8.7%, and 13.0% for these fractions, respectively, during the 20-minute observation period. The results of this study show that E. longifolia Jack extracts can increase libido in middle-aged male rats.
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eurycolactones a c novel quassinoids from Eurycoma longifolia
Tetrahedron Letters, 2000Co-Authors: Hooi Hoon Ang, Yukio Hitotsuyanagi, Koichi TakeyaAbstract:Abstract Three novel quassinoids, eurycolactones A–C (1–3), were isolated from the roots of Eurycoma longifolia Jack. Their structures were elucidated by interpretation of the spectroscopic data.
Widyarini Sitarina - One of the best experts on this subject based on the ideXlab platform.
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The Effect of Pasak Bumi (Eurycoma longifolia, Jack) Roots Ethanol Extract against Hematology Profile of Healthy Volunteers
'Universitas Gadjah Mada', 2019Co-Authors: Nurani, Laela Hayu, Azzahra Fara, Widyarini Sitarina, Rohman AbdulAbstract:The roots of Eurycoma longifolia, Jack has a lot of beneficial activity on human health. The use of Eurycoma longifolia, Jack roots as a traditional medicine should be made in the form of a dosage that is more effective, safe, and had no side effects, especially on hematology. Easy preparations are capsule. This study aimed at identifying the effect of ethanol extract capsule of Eurycoma longifolia, Jack roots on hematology of healthy volunteers. Healthy volunteers were assigned in the study, each of which were 10 male and 10 female who met the inclusion criteria. Both groups were given ethanol extract capsule of Eurycoma longifolia, Jack roots for 14 days, with the dose of 300 mg extract once a day, after meal, at night. Hematology examination was performed on day 0; 14 and 42. Results were compared statistically using Repeated ANOVA and Friedman tests. The result of the study indicated that the average value of hematology at the examination day of 0; 14; and 42, there were significant differences in MCV and leukocyte parameters in healthy male volunteers (p
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Cek Similarity "Formulasi Kapsul Ekstrak Etanol Akar Pasak Bumi (Eurycoma longifolia Jack.,)dan Pengaruhnya terhadap Vital Sign Manusia Sehat"
Fakultas Farmasi Universitas Gadjah Mada, 2017Co-Authors: Nurani, Laela Hayu, Rohman Abdul, Kumalasari Eka, Widyarini SitarinaAbstract:Pasak bumi (Eurycoma longifolia) has the potential to be developed as antihypertensive, antipyretic, aphrodisiacs and health supplements. The use of E. longifolia as a traditional medicine needs to be pursued in the form of more effective and appropriate formulation. The capsule preparations are easy to make and can cover the bitter taste of E. longifolia. Clinical trials in this study use design pre-post treatment in healthy humans. Subjects used were male - healthy men and healthy women who met inclusion criteria and were subjected with formulated capsule for 14 days. The study resulted capsule formula comprising of the ethanolic extract of E. longifolia 300 mg, vivapur 101 300 mg, 58 mg maydis starch, aerosil 3%, talc 2%, and Mg stearate 1%. The results showed that the capsule of E. longifolia did not affect the value of heart rate, respiration rate, body temperature and weight (P > 0.05), based on paired t-test, but they causes a decrease in blood pressure of healthy human. The ethanol extract of E. longifolia caused vasodilation of blood vessels that can be used in antihypertensive therapy. Keywords: Eurycoma longifolia, capsules, vital sign
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Hasil Peer Review Teman Sejawat "Formulasi Kapsul Ekstrak Etanol Akar Pasak Bumi(Eurycoma longifolia Jack.,)dan Pengaruhnya terhadap Vital Sign Manusia Sehat"
Fakultas Farmasi UGM, 2017Co-Authors: Nurani, Laela Hayu, Rohman Abdul, Kumalasari Eka, Widyarini SitarinaAbstract:Pasak bumi (Eurycoma longifolia) has the potential to be developed as antihypertensive, antipyretic, aphrodisiacs and health supplements. The use of E. longifolia as a traditional medicine needs to be pursued in the form of more effective and appropriate formulation. The capsule preparations are easy to make and can cover the bitter taste of E. longifolia. Clinical trials in this study use design pre-post treatment in healthy humans. Subjects used were male - healthy men and healthy women who met inclusion criteria and were subjected with formulated capsule for 14 days. The study resulted capsule formula comprising of the ethanolic extract of E. longifolia 300 mg, vivapur 101 300 mg, 58 mg maydis starch, aerosil 3%, talc 2%, and Mg stearate 1%. The results showed that the capsule of E. longifolia did not affect the value of heart rate, respiration rate, body temperature and weight (P > 0.05), based on paired t-test, but they causes a decrease in blood pressure of healthy human. The ethanol extract of E. longifolia caused vasodilation of blood vessels that can be used in antihypertensive therapy. Keywords: Eurycoma longifolia, capsules, vital sign
Nurani, Laela Hayu - One of the best experts on this subject based on the ideXlab platform.
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The Effect of Pasak Bumi (Eurycoma longifolia, Jack) Roots Ethanol Extract against Hematology Profile of Healthy Volunteers
'Universitas Gadjah Mada', 2019Co-Authors: Nurani, Laela Hayu, Azzahra Fara, Widyarini Sitarina, Rohman AbdulAbstract:The roots of Eurycoma longifolia, Jack has a lot of beneficial activity on human health. The use of Eurycoma longifolia, Jack roots as a traditional medicine should be made in the form of a dosage that is more effective, safe, and had no side effects, especially on hematology. Easy preparations are capsule. This study aimed at identifying the effect of ethanol extract capsule of Eurycoma longifolia, Jack roots on hematology of healthy volunteers. Healthy volunteers were assigned in the study, each of which were 10 male and 10 female who met the inclusion criteria. Both groups were given ethanol extract capsule of Eurycoma longifolia, Jack roots for 14 days, with the dose of 300 mg extract once a day, after meal, at night. Hematology examination was performed on day 0; 14 and 42. Results were compared statistically using Repeated ANOVA and Friedman tests. The result of the study indicated that the average value of hematology at the examination day of 0; 14; and 42, there were significant differences in MCV and leukocyte parameters in healthy male volunteers (p
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Cek Similarity "Formulasi Kapsul Ekstrak Etanol Akar Pasak Bumi (Eurycoma longifolia Jack.,)dan Pengaruhnya terhadap Vital Sign Manusia Sehat"
Fakultas Farmasi Universitas Gadjah Mada, 2017Co-Authors: Nurani, Laela Hayu, Rohman Abdul, Kumalasari Eka, Widyarini SitarinaAbstract:Pasak bumi (Eurycoma longifolia) has the potential to be developed as antihypertensive, antipyretic, aphrodisiacs and health supplements. The use of E. longifolia as a traditional medicine needs to be pursued in the form of more effective and appropriate formulation. The capsule preparations are easy to make and can cover the bitter taste of E. longifolia. Clinical trials in this study use design pre-post treatment in healthy humans. Subjects used were male - healthy men and healthy women who met inclusion criteria and were subjected with formulated capsule for 14 days. The study resulted capsule formula comprising of the ethanolic extract of E. longifolia 300 mg, vivapur 101 300 mg, 58 mg maydis starch, aerosil 3%, talc 2%, and Mg stearate 1%. The results showed that the capsule of E. longifolia did not affect the value of heart rate, respiration rate, body temperature and weight (P > 0.05), based on paired t-test, but they causes a decrease in blood pressure of healthy human. The ethanol extract of E. longifolia caused vasodilation of blood vessels that can be used in antihypertensive therapy. Keywords: Eurycoma longifolia, capsules, vital sign
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Hasil Peer Review Teman Sejawat "Formulasi Kapsul Ekstrak Etanol Akar Pasak Bumi(Eurycoma longifolia Jack.,)dan Pengaruhnya terhadap Vital Sign Manusia Sehat"
Fakultas Farmasi UGM, 2017Co-Authors: Nurani, Laela Hayu, Rohman Abdul, Kumalasari Eka, Widyarini SitarinaAbstract:Pasak bumi (Eurycoma longifolia) has the potential to be developed as antihypertensive, antipyretic, aphrodisiacs and health supplements. The use of E. longifolia as a traditional medicine needs to be pursued in the form of more effective and appropriate formulation. The capsule preparations are easy to make and can cover the bitter taste of E. longifolia. Clinical trials in this study use design pre-post treatment in healthy humans. Subjects used were male - healthy men and healthy women who met inclusion criteria and were subjected with formulated capsule for 14 days. The study resulted capsule formula comprising of the ethanolic extract of E. longifolia 300 mg, vivapur 101 300 mg, 58 mg maydis starch, aerosil 3%, talc 2%, and Mg stearate 1%. The results showed that the capsule of E. longifolia did not affect the value of heart rate, respiration rate, body temperature and weight (P > 0.05), based on paired t-test, but they causes a decrease in blood pressure of healthy human. The ethanol extract of E. longifolia caused vasodilation of blood vessels that can be used in antihypertensive therapy. Keywords: Eurycoma longifolia, capsules, vital sign
