The Experts below are selected from a list of 6564 Experts worldwide ranked by ideXlab platform
Jian Feng - One of the best experts on this subject based on the ideXlab platform.
-
physiological characterization of aluminum tolerance and accumulation in tartary and wild buckwheat
New Phytologist, 2015Co-Authors: Hua Wang, Rong Fu Chen, Takashi Iwashita, Ren Fang Shen, Jian FengAbstract:Summary Ionic aluminum (Al) is toxic for plant growth, but some plant species are able to accumulate Al at high concentrations without showing toxicity symptoms. In order to determine whether other species in the genus Fagopyrum are able to accumulate Al like common buckwheat (Fagopyrum esculentum), we investigated the external and internal detoxification mechanisms of Al in two self-compatible species: tartary (Fagopyrum tataricum) and wild buckwheat (Fagopyrum homotropicum). Both tartary and wild buckwheat showed high Al tolerance comparable to common buckwheat. Furthermore, these two species also secreted oxalate rapidly from the roots in response to Al in a time-dependent manner. Both tartary and wild buckwheat accumulated > 1 mg g−1 Al in the leaves after short-term exposure to Al. Analysis with 27Al-nuclear magnetic resonance (NMR) revealed that Al was present in the form of Al-oxalate (1 : 3 ratio) in the roots and leaves, but in the form of Al-citrate (1 : 1 ratio) in the xylem sap in both species. These results indicate that similar to common buckwheat, both tartary and wild buckwheat detoxify Al externally and internally, respectively, by secreting oxalate from the roots and by forming the Al-oxalate complex, which is a nonphytotoxic form. These features of Al response and accumulation may be conserved in genus Fagopyrum.
Maria D Logacheva - One of the best experts on this subject based on the ideXlab platform.
-
de novo sequencing and characterization of floral transcriptome in two species of buckwheat Fagopyrum
BMC Genomics, 2011Co-Authors: Maria D Logacheva, Artem S Kasianov, Dmitriy V Vinogradov, Tagir H Samigullin, Mikhail S Gelfand, Vsevolod J Makeev, Aleksey A PeninAbstract:Background Transcriptome sequencing data has become an integral component of modern genetics, genomics and evolutionary biology. However, despite advances in the technologies of DNA sequencing, such data are lacking for many groups of living organisms, in particular, many plant taxa. We present here the results of transcriptome sequencing for two closely related plant species. These species, Fagopyrum esculentum and F. tataricum, belong to the order Caryophyllales - a large group of flowering plants with uncertain evolutionary relationships. F. esculentum (common buckwheat) is also an important food crop. Despite these practical and evolutionary considerations Fagopyrum species have not been the subject of large-scale sequencing projects.
-
comparative chloroplast genomics and phylogenetics of Fagopyrum esculentum ssp ancestrale a wild ancestor of cultivated buckwheat
BMC Plant Biology, 2008Co-Authors: Maria D Logacheva, Tahir H Samigullin, Amit Dhingra, Aleksey A PeninAbstract:Chloroplast genome sequences are extremely informative about species-interrelationships owing to its non-meiotic and often uniparental inheritance over generations. The subject of our study, Fagopyrum esculentum, is a member of the family Polygonaceae belonging to the order Caryophyllales. An uncertainty remains regarding the affinity of Caryophyllales and the asterids that could be due to undersampling of the taxa. With that background, having access to the complete chloroplast genome sequence for Fagopyrum becomes quite pertinent. We report the complete chloroplast genome sequence of a wild ancestor of cultivated buckwheat, Fagopyrum esculentum ssp. ancestrale. The sequence was rapidly determined using a previously described approach that utilized a PCR-based method and employed universal primers, designed on the scaffold of multiple sequence alignment of chloroplast genomes. The gene content and order in buckwheat chloroplast genome is similar to Spinacia oleracea. However, some unique structural differences exist: the presence of an intron in the rpl2 gene, a frameshift mutation in the rpl23 gene and extension of the inverted repeat region to include the ycf1 gene. Phylogenetic analysis of 61 protein-coding gene sequences from 44 complete plastid genomes provided strong support for the sister relationships of Caryophyllales (including Polygonaceae) to asterids. Further, our analysis also provided support for Amborella as sister to all other angiosperms, but interestingly, in the bayesian phylogeny inference based on first two codon positions Amborella united with Nymphaeales. Comparative genomics analyses revealed that the Fagopyrum chloroplast genome harbors the characteristic gene content and organization as has been described for several other chloroplast genomes. However, it has some unique structural features distinct from previously reported complete chloroplast genome sequences. Phylogenetic analysis of the dataset, including this new sequence from non-core Caryophyllales supports the sister relationship between Caryophyllales and asterids.
Aleksey A Penin - One of the best experts on this subject based on the ideXlab platform.
-
de novo sequencing and characterization of floral transcriptome in two species of buckwheat Fagopyrum
BMC Genomics, 2011Co-Authors: Maria D Logacheva, Artem S Kasianov, Dmitriy V Vinogradov, Tagir H Samigullin, Mikhail S Gelfand, Vsevolod J Makeev, Aleksey A PeninAbstract:Background Transcriptome sequencing data has become an integral component of modern genetics, genomics and evolutionary biology. However, despite advances in the technologies of DNA sequencing, such data are lacking for many groups of living organisms, in particular, many plant taxa. We present here the results of transcriptome sequencing for two closely related plant species. These species, Fagopyrum esculentum and F. tataricum, belong to the order Caryophyllales - a large group of flowering plants with uncertain evolutionary relationships. F. esculentum (common buckwheat) is also an important food crop. Despite these practical and evolutionary considerations Fagopyrum species have not been the subject of large-scale sequencing projects.
-
comparative chloroplast genomics and phylogenetics of Fagopyrum esculentum ssp ancestrale a wild ancestor of cultivated buckwheat
BMC Plant Biology, 2008Co-Authors: Maria D Logacheva, Tahir H Samigullin, Amit Dhingra, Aleksey A PeninAbstract:Chloroplast genome sequences are extremely informative about species-interrelationships owing to its non-meiotic and often uniparental inheritance over generations. The subject of our study, Fagopyrum esculentum, is a member of the family Polygonaceae belonging to the order Caryophyllales. An uncertainty remains regarding the affinity of Caryophyllales and the asterids that could be due to undersampling of the taxa. With that background, having access to the complete chloroplast genome sequence for Fagopyrum becomes quite pertinent. We report the complete chloroplast genome sequence of a wild ancestor of cultivated buckwheat, Fagopyrum esculentum ssp. ancestrale. The sequence was rapidly determined using a previously described approach that utilized a PCR-based method and employed universal primers, designed on the scaffold of multiple sequence alignment of chloroplast genomes. The gene content and order in buckwheat chloroplast genome is similar to Spinacia oleracea. However, some unique structural differences exist: the presence of an intron in the rpl2 gene, a frameshift mutation in the rpl23 gene and extension of the inverted repeat region to include the ycf1 gene. Phylogenetic analysis of 61 protein-coding gene sequences from 44 complete plastid genomes provided strong support for the sister relationships of Caryophyllales (including Polygonaceae) to asterids. Further, our analysis also provided support for Amborella as sister to all other angiosperms, but interestingly, in the bayesian phylogeny inference based on first two codon positions Amborella united with Nymphaeales. Comparative genomics analyses revealed that the Fagopyrum chloroplast genome harbors the characteristic gene content and organization as has been described for several other chloroplast genomes. However, it has some unique structural features distinct from previously reported complete chloroplast genome sequences. Phylogenetic analysis of the dataset, including this new sequence from non-core Caryophyllales supports the sister relationship between Caryophyllales and asterids.
Ji-rong Shao - One of the best experts on this subject based on the ideXlab platform.
-
psbE-psbL and ndhA Intron, the Promising Plastid DNA Barcode of Fagopyrum.
International journal of molecular sciences, 2019Co-Authors: Huang Yue, Ji-rong Shao, Chenglong Wang, Li Zhiqiang, Chen-yan Zou, Wen Wen, Xue-mei ZhuAbstract:Buckwheat is an important functional food material with high nutritional value. However, it is still a difficult task for the taxonomy studies of wild buckwheat that are only based on morphology. In order to demonstrate the most efficient DNA barcode in the phylogenetic research of buckwheat, promote the investigation of wild buckwheat, and also reveal the phylogenetic relationship between Fagopyrum species, psbE-psbL and ndhA intron were validated here, which previously have been proved to be promising DNA barcode candidates for phylogenetic studies in genera Fagopyrum. Meanwhile, ndhA intron + psbE-psbL and matK + psbE-psbL could distinguish the relationship between species clearly. Combining the results of morphology and molecular markers, we suggested the buckwheat species should be divided into two subgroups, one subgroup consisted of F. tataricum, F. esculentum, F. cymosum and its related wild species, and the other subgroup included other wild buckwheat species. Our results could fulfill molecular markers of taxonomy research in genera Fagopyrum, promote wild buckwheat species identification, and assist in the use of wild buckwheat resources in the future. Additionally, the phylogenetic relationship revealed here could provide valuable information for molecular breeding of buckwheat and provide reference for inter-species hybridization.
-
Description of Cultivated Tartary Buckwheat
Buckwheat Germplasm in the World, 2018Co-Authors: Meiliang Zhou, Chen Ruan, Mengqi Ding, Ji-rong Shao, Xianyu Deng, Yu Tang, Yi-xiong TangAbstract:Abstract Tartary buckwheat is one of the cultivars of Fagopyrum , whose species name is Fagopyrum tataricum (L) Gaertn and whose English name is Tartary buckwheat. Tartary buckwheat is called phapar in India, tite phapar in Nepal, and bjo in Bhutan. In China and Nepal, it is also called bitter buckwheat. Tartary buckwheat is mainly grown in the south of China, India, the southern Himalayas, Nepal, Bhutan, and Pakistan, etc. The grains of Tartary buckwheat are rich in proteins, fats, vitamins, and minerals, as well as rutin, quercitin, and other flavonoids that other Gramineae crops don’t contain. Hence, Tartary buckwheat has considerable nutritional and medicinal values, which is considered as an ideal functional food source for humans.
-
Perennial Self-Incompatible Wild Fagopyrum Species
Buckwheat Germplasm in the World, 2018Co-Authors: Meiliang Zhou, Chen Ruan, Mengqi Ding, Ji-rong Shao, Xianyu Deng, Yu Tang, Yi-xiong TangAbstract:Abstract In this chapter, the botanical descriptions of Fagopyrum cymosum, Fagopyrum urophyllum, and Fagopyrum statice are presented, and the global distributions, habitats, and growth habits of these perennial self-incompatible wild Fagopyrum species are described. Fagopyrum cymosum has been known about for a long time, and its changing nomenclature throughout the years is also presented in the chapter.
-
Perennial Self-Compatible Wild Fagopyrum Species: F. hailuogouense
Buckwheat Germplasm in the World, 2018Co-Authors: Meiliang Zhou, Chen Ruan, Mengqi Ding, Ji-rong Shao, Xianyu Deng, Yu Tang, Yi-xiong TangAbstract:Abstract Fagopyrum hailuogouense is a perennial self-compatible wild Fagopyrum species. In this chapter, the botanical description, global distribution, habitat and growth habits of F. hailuogouense are described.
-
Fagopyrum luojishanense, a New Species of Polygonaceae from Sichuan, China
Novon: A Journal for Botanical Nomenclature, 2015Co-Authors: Ling-li Hou, Meiliang Zhou, Yu Tang, Xue-mei Zhu, Qian Zhang, Xiong-bang Yang, Ji-rong ShaoAbstract:Fagopyrum luojishanense J. R. Shao, a new species of Fagopyrum Mill. (Polygonaceae), is described and illustrated from Liangshan in Sichuan Province, China. This species is distinguished by its leaf blades that are ovate, narrowly ovate or triangular, winged seeds, and inflorescences that are either axillary or terminal, and we further distinguish the new species based on karyotype analysis. Fruit surface ornamentation of F. luojishanense is densely and irregularly warty, while fruit ornamentation is sparser in F. gracilipes (Hemsl.) Dammer ex Diels. Fagopyrum luojishanense is a diploid taxon, with 2n = 2x = 16 and a karyotype of 16 metacentric chromosomes, while F. gracilipes is tetraploid, 4n = 4x = 32, with a karyotype of 30 metacentric and two submetacentric chromosomes.
Hua Wang - One of the best experts on this subject based on the ideXlab platform.
-
genome wide transcriptomic and phylogenetic analyses reveal distinct aluminum tolerance mechanisms in the aluminum accumulating species buckwheat Fagopyrum tataricum
BMC Plant Biology, 2015Co-Authors: Haifeng Zhu, Hua Wang, Yifang Zhu, Jianwen Zou, Fangjie Zhao, Chaofeng HuangAbstract:Background Similar to common buckwheat (Fagopyrum esculentum), tartary buckwheat (Fagopyrum tataricum) shows a high level of aluminum (Al) tolerance and accumulation. However, the molecular mechanisms for Al detoxification and accumulation are still poorly understood. To begin to elucidate the molecular basis of Al tolerance and accumulation, we used the Illumina high-throughput mRNA sequencing (RNA-seq) technology to conduct a genome-wide transcriptome analysis on both tip and basal segments of the roots exposed to Al.
-
physiological characterization of aluminum tolerance and accumulation in tartary and wild buckwheat
New Phytologist, 2015Co-Authors: Hua Wang, Rong Fu Chen, Takashi Iwashita, Ren Fang Shen, Jian FengAbstract:Summary Ionic aluminum (Al) is toxic for plant growth, but some plant species are able to accumulate Al at high concentrations without showing toxicity symptoms. In order to determine whether other species in the genus Fagopyrum are able to accumulate Al like common buckwheat (Fagopyrum esculentum), we investigated the external and internal detoxification mechanisms of Al in two self-compatible species: tartary (Fagopyrum tataricum) and wild buckwheat (Fagopyrum homotropicum). Both tartary and wild buckwheat showed high Al tolerance comparable to common buckwheat. Furthermore, these two species also secreted oxalate rapidly from the roots in response to Al in a time-dependent manner. Both tartary and wild buckwheat accumulated > 1 mg g−1 Al in the leaves after short-term exposure to Al. Analysis with 27Al-nuclear magnetic resonance (NMR) revealed that Al was present in the form of Al-oxalate (1 : 3 ratio) in the roots and leaves, but in the form of Al-citrate (1 : 1 ratio) in the xylem sap in both species. These results indicate that similar to common buckwheat, both tartary and wild buckwheat detoxify Al externally and internally, respectively, by secreting oxalate from the roots and by forming the Al-oxalate complex, which is a nonphytotoxic form. These features of Al response and accumulation may be conserved in genus Fagopyrum.
