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Howard Thomas - One of the best experts on this subject based on the ideXlab platform.

  • introgression of crown rust puccinia coronata resistance from meadow fescue Festuca Pratensis into italian ryegrass lolium multiflorum genetic mapping and identification of associated molecular markers
    Plant Pathology, 2006
    Co-Authors: Ian P Armstead, I P King, W G Morgan, J A Harper, Howard Thomas, Leif Skot, Lesley B Turner, Mervyn O Humphreys, H W Roderick
    Abstract:

    Crown rust (Puccinia coronata) resistance (CRres), which had been introgressed from meadow fescue (Festuca Pratensis) into the Italian ryegrass (Lolium multiflorum) background, was genetically mapped with amplified fragment length polymorphism (AFLP) and sequence tagged site (STS) markers to a terminal segment of chromosome 5. Comparative mapping had previously shown that this region of the Lolium/Festuca genome has a degree of conserved genetic synteny with chromosomes 11 and 12 of rice. Sequences from rice chromosome 12 were used as templates for identifying further STS markers that cosegregated with CRres. The relative genomic positions of molecular markers associated with CRres in L. multiflorum, L. perenne, F. Pratensis and oats is discussed, along with their relationships to physical positions on rice chromosomes C11 and C12.

  • molecular tagging of a senescence gene by introgression mapping of a stay green mutation from Festuca Pratensis
    New Phytologist, 2004
    Co-Authors: B J Moore, Ian P Armstead, J A Harper, Howard Thomas, Iain S Donnison, Julie King, R N Jones, T Jones, Huw M Thomas, W G Morgan
    Abstract:

    B. J. Moore, I. S. Donnison, J. A. Harper, I. P. Armstead, J. King, H. Thomas, R. N. Jones, T. H. Jones, H. M. Thomas, W. G. Morgan, A. Thomas, H. J. Ougham, L. Huang, T. Fentem, L. A. Roberts and I. P. King (2005). Molecular tagging of a senescence gene by introgression mapping of a stay-green mutation from Festuca Pratensis. New Phytologist, 165(3) pp.801-806 Sponsorship: BBSRC RAE2008

  • a demonstration of a 1 1 correspondence between chiasma frequency and recombination using a lolium perenne Festuca Pratensis substitution
    Genetics, 2002
    Co-Authors: Julie King, W G Morgan, Ian P Armstead, Howard Thomas, R N Jones, M J Kearsey, L A Roberts, Lin Huang, I P King
    Abstract:

    A single chromosome of the grass species Festuca Pratensis has been introgressed into Lolium perenne to produce a diploid monosomic substitution line 2n = 2x = 14. The chromatin of F. Pratensis and L. perenne can be distinguished by genomic in situ hybridization (GISH), and it is therefore possible to visualize the substituted F. Pratensis chromosome in the L. perenne background and to study chiasma formation in a single marked bivalent. Recombination occurs freely in the F. Pratensis/L. perenne bivalent, and chiasma frequency counts give a predicted map length for this bivalent of 76 cM. The substituted F. Pratensis chromosome was also mapped with 104 EcoRI/Tru91 and HindIII/Tru91 amplified fragment length polymorphisms (AFLPs), generating a marker map of 81 cM. This map length is almost identical to the map length of 76 cM predicted from the chiasma frequency data. The work demonstrates a 1:1 correspondence between chiasma frequency and recombination and, in addition, the absence of chromatid interference across the Festuca and Lolium centromeres.

  • A Demonstration of a 1:1 Correspondence Between Chiasma Frequency and Recombination Using a Lolium perenne/Festuca Pratensis Substitution
    Genetics, 2002
    Co-Authors: Julie King, W G Morgan, Ian P Armstead, Howard Thomas, R N Jones, M J Kearsey, L A Roberts, Lin Huang, Ian P. King
    Abstract:

    A single chromosome of the grass species Festuca Pratensis has been introgressed into Lolium perenne to produce a diploid monosomic substitution line 2n = 2x = 14. The chromatin of F. Pratensis and L. perenne can be distinguished by genomic in situ hybridization (GISH), and it is therefore possible to visualize the substituted F. Pratensis chromosome in the L. perenne background and to study chiasma formation in a single marked bivalent. Recombination occurs freely in the F. Pratensis/L. perenne bivalent, and chiasma frequency counts give a predicted map length for this bivalent of 76 cM. The substituted F. Pratensis chromosome was also mapped with 104 EcoRI/Tru91 and HindIII/Tru91 amplified fragment length polymorphisms (AFLPs), generating a marker map of 81 cM. This map length is almost identical to the map length of 76 cM predicted from the chiasma frequency data. The work demonstrates a 1:1 correspondence between chiasma frequency and recombination and, in addition, the absence of chromatid interference across the Festuca and Lolium centromeres.

  • genetic and physical analysis of a single Festuca Pratensis chromosome segment substitution in lolium perenne
    Chromosoma, 2001
    Co-Authors: Ian P Armstead, I P King, W G Morgan, J A Harper, M D Hayward, John W Forster, R N Jones, Andrea L Bollard, Howard Thomas
    Abstract:

    Molecular marker analysis and genomic in situ hybridisation (GISH) were used to examine the process of chromosome segment introgression in BC2 diploid hybrids (2n=2x=14) between Lolium perenne and Festuca Pratensis. Two genotypes having what appeared to be the same, single, introgressed chromosome segment of F. Pratensis in the L. perenne background were crossed with diploid L. perenne to produce a recombinant series for the introgressed region. Physical and genetic analysis of this series showed that, while recombination seemed to be possible at all points along the chromosome arm, the rate of recombination varied depending on relative position: more recombination was detected in the interstitial region as compared with the centromeric or telomeric regions. The implications of these results for the use of GISH and molecular marker analysis in the measurement of linkage drag in backcross breeding programmes is discussed.

Arkadiusz Kosmala - One of the best experts on this subject based on the ideXlab platform.

  • Introgression mapping for genes of abiotic stress resistance in Lolium multiflorum and Festuca Pratensis
    Czech Journal of Genetics and Plant Breeding, 2020
    Co-Authors: Arkadiusz Kosmala, Marcin Rapacz, E Zwierzykowska, M W Humphreys, W. Joks, Z Zwierzykowski
    Abstract:

    Kosmala, A., Zwierzykowski, Z., Zwierzykowska, E., Rapacz, M., Joks, W., Humphreys, M. W. (2003). Introgression mapping for genes of abiotic stress resistance in Lolium multiflorum and Festuca Pratensis.  Czech Journal of Genetics and Plant Breeding, 39, (Special Issue), 342-344. Proceedings of the 25th EUCARPIA Fodder Crops and Amenity Grasses Section Meeting, Brno, Czech Republic, 1-4 September 2003.

  • karyotype reshufflings of Festuca Pratensis lolium perenne hybrids
    Protoplasma, 2018
    Co-Authors: Joanna Majka, Z Zwierzykowski, Maciej Majka, Arkadiusz Kosmala
    Abstract:

    Many different processes have an impact on the shape of plant karyotype. Recently, cytogenetic examination of Lolium species has revealed the occurrence of spontaneous fragile sites (FSs) associated with 35S rDNA regions. The FSs are defined as the chromosomal regions that are sensitive to forming gaps or breaks on chromosomes. The shape of karyotype can also be determined by interstitial telomeric sequences (ITSs), what was recognized for the first time in this paper in chromosomes of Festuca Pratensis × Lolium perenne hybrids. Both FSs and ITSs can contribute to genome instabilities and chromosome rearrangements. To evaluate whether these cytogenetic phenomena have an impact on karyotype reshuffling observed in Festuca × Lolium hybrids, we examined F1 F. Pratensis × L. perenne plants and generated F2-F9 progeny by fluorescent in situ hybridization (FISH) using rDNA sequences, telomere and centromere probes, as well as by genomic in situ hybridization (GISH). Analyses using a combination of FISH and GISH revealed that intergenomic rearrangements did not correspond to FSs but overlapped with ITSs for several analyzed genotypes. It suggests that internal telomeric repeats can affect the shape of F. Pratensis × L. perenne karyotypes. However, other factors that are involved in rearrangements and have a more crucial impact could exist, but they are still unknown.

  • Karyotype reshufflings of Festuca Pratensis × Lolium perenne hybrids.
    Protoplasma, 2017
    Co-Authors: Joanna Majka, Z Zwierzykowski, Maciej Majka, Arkadiusz Kosmala
    Abstract:

    Many different processes have an impact on the shape of plant karyotype. Recently, cytogenetic examination of Lolium species has revealed the occurrence of spontaneous fragile sites (FSs) associated with 35S rDNA regions. The FSs are defined as the chromosomal regions that are sensitive to forming gaps or breaks on chromosomes. The shape of karyotype can also be determined by interstitial telomeric sequences (ITSs), what was recognized for the first time in this paper in chromosomes of Festuca Pratensis × Lolium perenne hybrids. Both FSs and ITSs can contribute to genome instabilities and chromosome rearrangements. To evaluate whether these cytogenetic phenomena have an impact on karyotype reshuffling observed in Festuca × Lolium hybrids, we examined F1 F. Pratensis × L. perenne plants and generated F2-F9 progeny by fluorescent in situ hybridization (FISH) using rDNA sequences, telomere and centromere probes, as well as by genomic in situ hybridization (GISH). Analyses using a combination of FISH and GISH revealed that intergenomic rearrangements did not correspond to FSs but overlapped with ITSs for several analyzed genotypes. It suggests that internal telomeric repeats can affect the shape of F. Pratensis × L. perenne karyotypes. However, other factors that are involved in rearrangements and have a more crucial impact could exist, but they are still unknown.

  • exploiting repetitive sequences and bac clones in Festuca Pratensis karyotyping
    PLOS ONE, 2017
    Co-Authors: Joanna Majka, Tomasz Ksiązczyk, Agnieszka Kielbowiczmatuk, David Kopecký, Arkadiusz Kosmala
    Abstract:

    The Festuca genus is thought to be the most numerous genus of the Poaceae family. One of the most agronomically important forage grasses, Festuca Pratensis Huds. is treated as a model plant to study the molecular mechanisms associated with tolerance to winter stresses, including frost. However, the precise mapping of the genes governing stress tolerance in this species is difficult as its karyotype remains unrecognized. Only two F. Pratensis chromosomes with 35S and 5S rDNA sequences can be easily identified, but its remaining chromosomes have not been distinguished to date. Here, two libraries derived from F. Pratensis nuclear DNA with various contents of repetitive DNA sequences were used as sources of molecular probes for fluorescent in situ hybridisation (FISH), a BAC library and a library representing sequences most frequently present in the F. Pratensis genome. Using FISH, six groups of DNA sequences were revealed in chromosomes on the basis of their signal position, including dispersed-like sequences, chromosome painting-like sequences, centromeric-like sequences, knob-like sequences, a group without hybridization signals, and single locus-like sequences. The last group was exploited to develop cytogenetic maps of diploid and tetraploid F. Pratensis, which are presented here for the first time and provide a remarkable progress in karyotype characterization.

  • different mechanisms trigger an increase in freezing tolerance in Festuca Pratensis exposed to flooding stress
    Environmental and Experimental Botany, 2013
    Co-Authors: Barbara Jurczyk, Arkadiusz Kosmala, Tomasz Krepski, Marcin Rapacz
    Abstract:

    Abstract Increased precipitation and snow melt during warmer winters may lead to low temperature flooding and ice encasement formation. These conditions are stressful to plants and may affect their winter survival and spring regrowth. The aim of this study was to assess the effects of low temperature flooding on frost tolerance, photosynthetic performance, osmotic potential, water soluble carbohydrate content and expression of CBF6 , Cor14b and LOS2 genes in four genotypes of Festuca Pratensis with distinct levels of frost tolerance. It was shown that plants cold acclimated under flooding increase their frost tolerance faster and/or to a greater extent than in non-flooded controls. Changes in the induction kinetics of transcription factors encoding genes are connected with transient growth of frost tolerance in two out of the four genotypes, irrespective of their frost tolerance. A significant and stable increase in frost tolerance observed in the genotype with the lowest tolerance under control conditions was related to higher carbohydrate concentration in the flooded plants. In more frost tolerant genotypes, low temperature flooding also improved their resistance to low-temperature induced photoinhibition of photosynthesis. In conclusion, low-temperature flooding of the plant roots and crowns may boost cold acclimation efficiency in F. Pratensis , but this effect is genotype-dependent and varies according to the background.

W G Morgan - One of the best experts on this subject based on the ideXlab platform.

  • introgression of crown rust puccinia coronata resistance from meadow fescue Festuca Pratensis into italian ryegrass lolium multiflorum genetic mapping and identification of associated molecular markers
    Plant Pathology, 2006
    Co-Authors: Ian P Armstead, I P King, W G Morgan, J A Harper, Howard Thomas, Leif Skot, Lesley B Turner, Mervyn O Humphreys, H W Roderick
    Abstract:

    Crown rust (Puccinia coronata) resistance (CRres), which had been introgressed from meadow fescue (Festuca Pratensis) into the Italian ryegrass (Lolium multiflorum) background, was genetically mapped with amplified fragment length polymorphism (AFLP) and sequence tagged site (STS) markers to a terminal segment of chromosome 5. Comparative mapping had previously shown that this region of the Lolium/Festuca genome has a degree of conserved genetic synteny with chromosomes 11 and 12 of rice. Sequences from rice chromosome 12 were used as templates for identifying further STS markers that cosegregated with CRres. The relative genomic positions of molecular markers associated with CRres in L. multiflorum, L. perenne, F. Pratensis and oats is discussed, along with their relationships to physical positions on rice chromosomes C11 and C12.

  • molecular tagging of a senescence gene by introgression mapping of a stay green mutation from Festuca Pratensis
    New Phytologist, 2004
    Co-Authors: B J Moore, Ian P Armstead, J A Harper, Howard Thomas, Iain S Donnison, Julie King, R N Jones, T Jones, Huw M Thomas, W G Morgan
    Abstract:

    B. J. Moore, I. S. Donnison, J. A. Harper, I. P. Armstead, J. King, H. Thomas, R. N. Jones, T. H. Jones, H. M. Thomas, W. G. Morgan, A. Thomas, H. J. Ougham, L. Huang, T. Fentem, L. A. Roberts and I. P. King (2005). Molecular tagging of a senescence gene by introgression mapping of a stay-green mutation from Festuca Pratensis. New Phytologist, 165(3) pp.801-806 Sponsorship: BBSRC RAE2008

  • physical and genetic mapping in the grasses lolium perenne and Festuca Pratensis
    Genetics, 2002
    Co-Authors: Julie King, W G Morgan, Ian P Armstead, Iain S Donnison, R N Jones, Huw M Thomas, Ann Thomas, M J Kearsey, L A Roberts, I P King
    Abstract:

    A single chromosome of the grass species Festuca Pratensis has been introgressed into Lolium perenne to produce a diploid monosomic substitution line 2n = 2x = 14. In this line recombination occurs throughout the length of the F. Pratensis/L. perenne bivalent. The F. Pratensis chromosome and recombinants between it and its L. perenne homeologue can be visualized using genomic in situ hybridization (GISH). GISH junctions represent the physical locations of sites of recombination, enabling a range of recombinant chromosomes to be used for physical mapping of the introgressed F. Pratensis chromosome. The physical map, in conjunction with a genetic map composed of 104 F. Pratensis-specific amplified fragment length polymorphisms (AFLPs), demonstrated: (1) the first large-scale analysis of the physical distribution of AFLPs; (2) variation in the relationship between genetic and physical distance from one part of the F. Pratensis chromosome to another (e.g., variation was observed between and within chromosome arms); (3) that nucleolar organizer regions (NORs) and centromeres greatly reduce recombination; (4) that coding sequences are present close to the centromere and NORs in areas of low recombination in plant species with large genomes; and (5) apparent complete synteny between the F. Pratensis chromosome and rice chromosome 1.

  • a demonstration of a 1 1 correspondence between chiasma frequency and recombination using a lolium perenne Festuca Pratensis substitution
    Genetics, 2002
    Co-Authors: Julie King, W G Morgan, Ian P Armstead, Howard Thomas, R N Jones, M J Kearsey, L A Roberts, Lin Huang, I P King
    Abstract:

    A single chromosome of the grass species Festuca Pratensis has been introgressed into Lolium perenne to produce a diploid monosomic substitution line 2n = 2x = 14. The chromatin of F. Pratensis and L. perenne can be distinguished by genomic in situ hybridization (GISH), and it is therefore possible to visualize the substituted F. Pratensis chromosome in the L. perenne background and to study chiasma formation in a single marked bivalent. Recombination occurs freely in the F. Pratensis/L. perenne bivalent, and chiasma frequency counts give a predicted map length for this bivalent of 76 cM. The substituted F. Pratensis chromosome was also mapped with 104 EcoRI/Tru91 and HindIII/Tru91 amplified fragment length polymorphisms (AFLPs), generating a marker map of 81 cM. This map length is almost identical to the map length of 76 cM predicted from the chiasma frequency data. The work demonstrates a 1:1 correspondence between chiasma frequency and recombination and, in addition, the absence of chromatid interference across the Festuca and Lolium centromeres.

  • A Demonstration of a 1:1 Correspondence Between Chiasma Frequency and Recombination Using a Lolium perenne/Festuca Pratensis Substitution
    Genetics, 2002
    Co-Authors: Julie King, W G Morgan, Ian P Armstead, Howard Thomas, R N Jones, M J Kearsey, L A Roberts, Lin Huang, Ian P. King
    Abstract:

    A single chromosome of the grass species Festuca Pratensis has been introgressed into Lolium perenne to produce a diploid monosomic substitution line 2n = 2x = 14. The chromatin of F. Pratensis and L. perenne can be distinguished by genomic in situ hybridization (GISH), and it is therefore possible to visualize the substituted F. Pratensis chromosome in the L. perenne background and to study chiasma formation in a single marked bivalent. Recombination occurs freely in the F. Pratensis/L. perenne bivalent, and chiasma frequency counts give a predicted map length for this bivalent of 76 cM. The substituted F. Pratensis chromosome was also mapped with 104 EcoRI/Tru91 and HindIII/Tru91 amplified fragment length polymorphisms (AFLPs), generating a marker map of 81 cM. This map length is almost identical to the map length of 76 cM predicted from the chiasma frequency data. The work demonstrates a 1:1 correspondence between chiasma frequency and recombination and, in addition, the absence of chromatid interference across the Festuca and Lolium centromeres.

Marcin Rapacz - One of the best experts on this subject based on the ideXlab platform.

  • Introgression mapping for genes of abiotic stress resistance in Lolium multiflorum and Festuca Pratensis
    Czech Journal of Genetics and Plant Breeding, 2020
    Co-Authors: Arkadiusz Kosmala, Marcin Rapacz, E Zwierzykowska, M W Humphreys, W. Joks, Z Zwierzykowski
    Abstract:

    Kosmala, A., Zwierzykowski, Z., Zwierzykowska, E., Rapacz, M., Joks, W., Humphreys, M. W. (2003). Introgression mapping for genes of abiotic stress resistance in Lolium multiflorum and Festuca Pratensis.  Czech Journal of Genetics and Plant Breeding, 39, (Special Issue), 342-344. Proceedings of the 25th EUCARPIA Fodder Crops and Amenity Grasses Section Meeting, Brno, Czech Republic, 1-4 September 2003.

  • different mechanisms trigger an increase in freezing tolerance in Festuca Pratensis exposed to flooding stress
    Environmental and Experimental Botany, 2013
    Co-Authors: Barbara Jurczyk, Arkadiusz Kosmala, Tomasz Krepski, Marcin Rapacz
    Abstract:

    Abstract Increased precipitation and snow melt during warmer winters may lead to low temperature flooding and ice encasement formation. These conditions are stressful to plants and may affect their winter survival and spring regrowth. The aim of this study was to assess the effects of low temperature flooding on frost tolerance, photosynthetic performance, osmotic potential, water soluble carbohydrate content and expression of CBF6 , Cor14b and LOS2 genes in four genotypes of Festuca Pratensis with distinct levels of frost tolerance. It was shown that plants cold acclimated under flooding increase their frost tolerance faster and/or to a greater extent than in non-flooded controls. Changes in the induction kinetics of transcription factors encoding genes are connected with transient growth of frost tolerance in two out of the four genotypes, irrespective of their frost tolerance. A significant and stable increase in frost tolerance observed in the genotype with the lowest tolerance under control conditions was related to higher carbohydrate concentration in the flooded plants. In more frost tolerant genotypes, low temperature flooding also improved their resistance to low-temperature induced photoinhibition of photosynthesis. In conclusion, low-temperature flooding of the plant roots and crowns may boost cold acclimation efficiency in F. Pratensis , but this effect is genotype-dependent and varies according to the background.

  • similarities and differences in leaf proteome response to cold acclimation between Festuca Pratensis and lolium perenne
    2013
    Co-Authors: Arkadiusz Kosmala, Marcin Rapacz, A Bocian, Barbara Jurczyk, łukasz Marczak, Z Zwierzykowski
    Abstract:

    Lolium perenne is used extensively as a forage and turf grass due to its high nutritive values, rapid establishment and persistence. Festuca Pratensis, a species closely related to L. perenne, has lower nutritive values but is one of the most winter-hardy species within the Lolium-Festuca complex. Frost tolerance is the main component of winter-hardiness and the plant species growing in temperate regions can acquire it through exposure to low, non-lethal temperatures, a phenomenon known as cold acclimation. Herein, we review our recent results of two proteomic projects, focused on F. Pratensis and L. perenne. Each project involved the comparison of leaf protein accumulation profiles during cold acclimation between plants with different levels of frost tolerance by the use of two-dimensional electrophoresis and identification of differentially accumulated proteins by mass spectrometry. In the present paper similarities and differences in leaf proteome response to cold acclimation between F. Pratensis and L. perenne are summarized.

  • Introgression of genes for abiotic stress resistance from Festuca Pratensis and F. arundinacea into Lolium multiflorum germplasm
    2009
    Co-Authors: M. Skibinska, Arkadiusz Kosmala, Marcin Rapacz, M W Humphreys, W. Joks, Z Zwierzykowski
    Abstract:

    Kosmala, A., Skibinska, M., Zwierzykowski, Z., Humphreys, M. W., Rapacz, M., Joks, W. (2003). Introgression of genes for abiotic stress resistance from Festuca Pratensis and F. arundinacea into Lolium multiflorum germplasm. Vortage fur Pflanzenzuchtung, Proceedings 24th EUCARPIA Fodder Crops and Amenity Grasses Section Meeting, Braunschweig, Germany, 22-26 September 2002, 59, 225-231

  • Locating, and utilising Festuca Pratensis genes for winter hardiness for the future development of more persistent high quality Lolium cultivars
    2009
    Co-Authors: Arkadiusz Kosmala, Z Zwierzykowski, Dagmara Gasior, M W Humphreys, O. A. Rognli, Marcin Rapacz
    Abstract:

    Humphreys, M. W., Gasior, D., Kosmala, A., Rognli, O. A., Zwierzykowski, Z., Rapacz, M. (2005). Locating, and utilising Festuca Pratensis genes for winter hardiness for the future development of more persistent high quality Lolium cultivars. Page 160 in: Humphreys, M. O.(Ed.), Molecular Breeding for the Genetic Improvement of Forage Crops and Turf. Proceedings of the 4th International Symposium on the Molecular Breeding of Forage and Turf. XXth International Grassland Congress, July 2005, Aberystwyth, Wales.

Ian P Armstead - One of the best experts on this subject based on the ideXlab platform.

  • transmission frequencies of introgressed Festuca Pratensis chromosomes and chromosome segments in lolium perenne
    Crop Science, 2013
    Co-Authors: Julie King, J A Harper, Ian P Armstead, I P King
    Abstract:

    The introgression of genetic variation from related species into crops provides an important route by which superior plant varieties can be produced. The primary aim of introgression involves the transfer of a small chromosome segment from a related species into a chromosome of a crop species (via recombination at meiosis) to generate an interspecific recombinant chromosome. Very little is known about the selective pressures that act on the products of interspecific recombination. Seven monosomic substitution lines were developed between Lolium perenne and Festuca Pratensis. When each line was backcrossed to L. perenne recombination occurred between the F. Pratensis chromosome and its L. perenne homoeologue, resulting in backcross populations carrying L. perenne/F. Pratensis recombinant chromosomes. This paper describes the relationship between the frequency of generation of interspecific recombinant chromosomes with the frequency of their transmission to the next generation. The results reveal the presence of neutral, negative, and positive selection pressures for the transmission of F. Pratensis chromosomes and L. perenne/F. Pratensis recombinant chromosomes through the gametes to the next generation. The type of selection pressure observed depended on which linkage group the F. Pratensis chromosome under study was derived from. The implications of these results are discussed.

  • introgression of crown rust puccinia coronata resistance from meadow fescue Festuca Pratensis into italian ryegrass lolium multiflorum genetic mapping and identification of associated molecular markers
    Plant Pathology, 2006
    Co-Authors: Ian P Armstead, I P King, W G Morgan, J A Harper, Howard Thomas, Leif Skot, Lesley B Turner, Mervyn O Humphreys, H W Roderick
    Abstract:

    Crown rust (Puccinia coronata) resistance (CRres), which had been introgressed from meadow fescue (Festuca Pratensis) into the Italian ryegrass (Lolium multiflorum) background, was genetically mapped with amplified fragment length polymorphism (AFLP) and sequence tagged site (STS) markers to a terminal segment of chromosome 5. Comparative mapping had previously shown that this region of the Lolium/Festuca genome has a degree of conserved genetic synteny with chromosomes 11 and 12 of rice. Sequences from rice chromosome 12 were used as templates for identifying further STS markers that cosegregated with CRres. The relative genomic positions of molecular markers associated with CRres in L. multiflorum, L. perenne, F. Pratensis and oats is discussed, along with their relationships to physical positions on rice chromosomes C11 and C12.

  • identification of perennial ryegrass lolium perenne l and meadow fescue Festuca Pratensis huds candidate orthologous sequences to the rice hd1 se1 and barley hvco1 constans like genes through comparative mapping and microsynteny
    New Phytologist, 2005
    Co-Authors: Ian P Armstead, Iain S Donnison, Leif Skot, Lesley B Turner, Kirsten P Skot, Mervyn O Humphreys, I P King
    Abstract:

    I. P. Armstead , L. Skot , L. B. Turner , K. P. Skot , I. S. Donnison , M. O. Humphreys and I. P. King (2005) Identification of perennial ryegrass (Lolium perenne (L.)) and meadow fescue (Festuca Pratensis (Huds.)) candidate orthologous sequences to the rice Hd1(Se1) and barley HvCO1 CONSTANS-like genes through comparative mapping and microsynteny. New Phytologist, 167 (1) pp.239-247 Key words: CONSTANS, Festuca (Schedonorus) Pratensis (meadow fescue), flowering, Hd1, HvCO1, Lolium perenne (perennial ryegrass), LpCO, microsynteny. Sponsorship: BBSRC RAE2008

  • molecular tagging of a senescence gene by introgression mapping of a stay green mutation from Festuca Pratensis
    New Phytologist, 2004
    Co-Authors: B J Moore, Ian P Armstead, J A Harper, Howard Thomas, Iain S Donnison, Julie King, R N Jones, T Jones, Huw M Thomas, W G Morgan
    Abstract:

    B. J. Moore, I. S. Donnison, J. A. Harper, I. P. Armstead, J. King, H. Thomas, R. N. Jones, T. H. Jones, H. M. Thomas, W. G. Morgan, A. Thomas, H. J. Ougham, L. Huang, T. Fentem, L. A. Roberts and I. P. King (2005). Molecular tagging of a senescence gene by introgression mapping of a stay-green mutation from Festuca Pratensis. New Phytologist, 165(3) pp.801-806 Sponsorship: BBSRC RAE2008

  • physical and genetic mapping in the grasses lolium perenne and Festuca Pratensis
    Genetics, 2002
    Co-Authors: Julie King, W G Morgan, Ian P Armstead, Iain S Donnison, R N Jones, Huw M Thomas, Ann Thomas, M J Kearsey, L A Roberts, I P King
    Abstract:

    A single chromosome of the grass species Festuca Pratensis has been introgressed into Lolium perenne to produce a diploid monosomic substitution line 2n = 2x = 14. In this line recombination occurs throughout the length of the F. Pratensis/L. perenne bivalent. The F. Pratensis chromosome and recombinants between it and its L. perenne homeologue can be visualized using genomic in situ hybridization (GISH). GISH junctions represent the physical locations of sites of recombination, enabling a range of recombinant chromosomes to be used for physical mapping of the introgressed F. Pratensis chromosome. The physical map, in conjunction with a genetic map composed of 104 F. Pratensis-specific amplified fragment length polymorphisms (AFLPs), demonstrated: (1) the first large-scale analysis of the physical distribution of AFLPs; (2) variation in the relationship between genetic and physical distance from one part of the F. Pratensis chromosome to another (e.g., variation was observed between and within chromosome arms); (3) that nucleolar organizer regions (NORs) and centromeres greatly reduce recombination; (4) that coding sequences are present close to the centromere and NORs in areas of low recombination in plant species with large genomes; and (5) apparent complete synteny between the F. Pratensis chromosome and rice chromosome 1.