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Atanas Todorov Atanasov - One of the best experts on this subject based on the ideXlab platform.
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antiplatelet aggregation activity of a fraction isolated from Galega officinalis l
Journal of Herbs Spices & Medicinal Plants, 2003Co-Authors: Atanas Todorov Atanasov, Bojidar TchorbanovAbstract:ABSTRACT A fraction isolated from crude aqueous extract of Galega officinalis L. and purified by column chromatography inhibit platelet aggregation in platelet-rich plasma. The active fraction of the extract, molecular weight of 100–140 kDa, appeared to be polysaccharide-protein complex. Aggregation of platelets initiated by 25 μM ADP was inhibited 50 percent by 11.2 μg/ml of the fraction. Aggregation of platelets initiated by 100 μg/ml collagen and 0.8 U/ml thrombin was completely inhibited by 16 μg/ml and 18.3 μg/ml, respectively.
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Anti-platelet fraction from Galega officinalis L. inhibits platelet aggregation.
Journal of medicinal food, 2002Co-Authors: Atanas Todorov Atanasov, Bojidar TchorbanovAbstract:A fraction from crude extract of Galega officinalis L. was purified by gel filtration on Sephadex G-25, Sepharose 4B, and ion-exchange chromatography on diethylaminoethyl (DEAE)-cellulose. The frac...
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inhibiting and disaggregating effect of gel filtered Galega officinalis l herbal extract on platelet aggregation
Journal of Ethnopharmacology, 2000Co-Authors: Atanas Todorov Atanasov, Vasil SpasovAbstract:The in vitro inhibiting and disaggregating effect on platelet aggregation of a gel-fractionated herbal extract from Galega officinalis L. is examined. The obtained Sephadex G-25 filtered fraction was 35-36 times more active than the crude extract. The threshold concentration at which this fraction inhibits platelet aggregation (5-10% inhibition) by 50 microM adenosine 5'-diphosphate (ADP) is 4.5-5 microg per 1 ml platelet-rich plasma (PRP). At a concentration of 35 microg/ml PRP the fraction inhibits 50% of aggregation by ADP and at a concentration of 125 microg/ml PRP fully inhibits the aggregation of PRP by ADP. At a concentration of 40 microg/ml PRP the fraction inhibits initiation of platelet aggregation by 0.18 mg/ml collagen and at 50 microg/ml PRP inhibits the initiation of aggregation by 0.7 units/ml thrombin. The G-25 filtered fraction shows a strong disaggregating effect on aggregated PRP. At a concentration of 65-75 microg/ml PRP, the fraction is able to disaggregate the 50-53% of aggregated platelet-rich plasma by 50 microM ADP, and 25% of aggregated PRP by 0.18 mg/ml collagen.
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inhibiting effect of desalted extract from Galega officinalis l on platelet aggregation
Folia Medica, 1999Co-Authors: Atanas Todorov AtanasovAbstract:The inhibiting and disaggregating effect of desalted and fractionated herbal extract of Galega officinalis L. on platelet aggregation in vitro is studied. At a concentration of 35 micrograms/ml in a platelet-rich plasma (PRP) the fraction inhibits 50% of aggregation by ADP and at 125 micrograms/ml PRP it inhibits fully the aggregation of PRP by ADP. At a concentration of 40 micrograms/ml PRP the fraction inhibits the initiation of platelet aggregation by collagen and at 50 micrograms/ml PRP inhibits the initiation of aggregation by thrombin. At a concentration of 65 micrograms/ml PRP the fraction can disaggregate 50% of the aggregated platelet-rich plasma by ADP and 25% of aggregated PRP by collagen.
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an effect of Galega officinalis l extract on platelet aggregation in rats
Journal of Herbs Spices & Medicinal Plants, 1996Co-Authors: Atanas Todorov AtanasovAbstract:The effect of aqueous extracts of Galega officinalis L. on platelet aggregation was studied by examining the blood from rats given an intravenous application of extract in the caudal vein. Treatment of test animals with extract at a ratio of 1 part extract (25.1±3.8 mg/ml dry substance) per 40 parts blood, suppressed platelet aggregation as compared with untreated controls. Suppression of aggregation was lost by 3 h after injection of extract. The components with antiaggregating activity have not identified.
Kristina Lindstrom - One of the best experts on this subject based on the ideXlab platform.
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alleviation of salt stress of symbiotic Galega officinalis l goat s rue by co inoculation of rhizobium with root colonizing pseudomonas
Plant and Soil, 2013Co-Authors: Kristina Lindstrom, Dilfuza Egamberdieva, Gabriela Berg, Leena A RasanenAbstract:Background and aims Salt stress is an increasing problem in agricultural soils in many parts of the world, and salt tolerant cropping systems are in great demand. We investigated the effect of co-inoculation of Galega officinalis with Rhizobium Galegae and two plant growth promoting Pseudomonas species on plant growth, nodulation, and N content under salt stress.
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alleviation of salt stress of symbiotic Galega officinalis l goat s rue by co inoculation of rhizobium with root colonizing pseudomonas
Plant and Soil, 2013Co-Authors: Kristina Lindstrom, Dilfuza Egamberdieva, Gabriela Berg, Leena A RasanenAbstract:Salt stress is an increasing problem in agricultural soils in many parts of the world, and salt tolerant cropping systems are in great demand. We investigated the effect of co-inoculation of Galega officinalis with Rhizobium Galegae and two plant growth promoting Pseudomonas species on plant growth, nodulation, and N content under salt stress. The effect of inoculation with R. Galegae sv. officinalis HAMBI 1141 alone and in combination with the root-colonizing Pseudomonas extremorientalis TSAU20 or Pseudomonas trivialis 3Re27 on the growth of G. officinalis exposed to salt stress (50 and 75 mM NaCl) was studied under gnotobiotic and greenhouse conditions. The growth of goat’s rue was reduced at 50 and 75 mM NaCl both in the gnotobiotic sand system and in low-fertilized potting soil in the greenhouse. Co-inoculation of unstressed and salt-stressed goat’s rue with R. Galegae HAMBI 1141 and either P. extremorientalis TSAU20 or P. trivialis 3Re27 significantly improved root and shoot growth and increased nodulation of plant roots in both growth systems compared with plants inoculated with R. Galegae alone. The nitrogen content of co-inoculated plant roots was significantly increased at 75 mM NaCl in potting soil. Co-inoculation of G. officinalis with either of the two plant growth promoting (PGPR) Pseudomonas strains also improved root tip-colonization by R. Galegae cells. The co-inoculation of goat’s rue with Rhizobium and PGPR Pseudomonas strains alleviated salt stress of plants grown in NaCl-amended gnotobiotic sand systems and in potting soil in the greenhouse.
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symbiotic and genetic diversity of rhizobium Galegae isolates collected from the Galega orientalis gene center in the caucasus
Applied and Environmental Microbiology, 2003Co-Authors: Evgeny E Andronov, Zewdu Terefework, M L Roumiantseva, N I Dzyubenko, O P Onichtchouk, O N Kurchak, Aneta Dreslernurmi, J P W Young, B V Simarov, Kristina LindstromAbstract:This paper explores the relationship between the genetic diversity of rhizobia and the morphological diversity of their plant hosts. Rhizobium Galegae strains were isolated from nodules of wild Galega orientalis and Galega officinalis in the Caucasus, the center of origin for G. orientalis. All 101 isolates were characterized by genomic amplified fragment length polymorphism fingerprinting and by PCR-restriction fragment length polymorphism (RFLP) of the rRNA intergenic spacer and of five parts of the symbiotic region adjacent to nod box sequences. By all criteria, the R. Galegae bv. officinalis and R. Galegae bv. orientalis strains form distinct clusters. The nod box regions are highly conserved among strains belonging to each of the two biovars but differ structurally to various degrees between the biovars. The findings suggest varying evolutionary pressures in different parts of the symbiotic genome of closely related R. Galegae biovars. Sixteen R. Galegae bv. orientalis strains harbored copies of the same insertion sequence element; all were isolated from a particular site and belonged to a limited range of chromosomal genotypes. In all analyses, the Caucasian R. Galegae bv. orientalis strains were more diverse than R. Galegae bv. officinalis strains, in accordance with the gene center theory.
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AFLP fingerprinting as a tool to study the genetic diversity of Rhizobium Galegae isolated from Galega orientalis and Galega officinalis.
Journal of biotechnology, 2001Co-Authors: Zewdu Terefework, S Kaijalainen, Kristina LindstromAbstract:AFLP fingerprints of Rhizobium Galegae strains that infect Galega orientalis and Galega officinalis obtained from different geographical sources, and of taxonomically diverse rhizobia representing the recognized species, were generated. Comparisons of the fingerprints from fluorescent labeled AFLP products using capillary electrophoresis on ABI prism 310, slab gel electrophoresis on ABI prism 377 genetic analyzers and silver staining were in good agreement. All methods delineated the G. orientalis strains from G. officinalis strains, the G. orientalis strains formed a tight cluster whereas the G. officinalis strains seem to show a greater level of genetic diversity. Comparison of fluorescent AFLP with other detection methods revealed that fluorescent labeling is more sensitive and practical, in addition, the deleterious effect of radioactivity associated with 32P-labeling, the delicate process of blotting polyacrylamide gels or the tedious procedure of silver staining can be avoided. The automated system facilitated a large number of runs at a time and the subsequent analysis of the data by generating exportable raw data. The congruency of the experiments was analyzed using the Bionumerics software.
Bruno Burlando - One of the best experts on this subject based on the ideXlab platform.
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Therapeutic Potential of Temperate Forage Legumes: A Review.
Critical reviews in food science and nutrition, 2016Co-Authors: Laura Cornara, Jianbo Xiao, Bruno BurlandoAbstract:The discovery of bioactive molecules from botanical sources is an expanding field, preferentially oriented to plants having a tradition of use in medicine and providing high yields and availability. Temperate forage legumes are Fabaceae species that include worldwide-important crops. These plants possess therapeutic virtues that have not only been used in veterinary and folk medicine, but have also attracted the interest of official medicine. We have examined here Medicago sativa (alfalfa), Trifolium pratense and T. repens (clovers), Melilotus albus and M. officinalis (sweet clovers), Lotus corniculatus (birdsfoot trefoil), Onobrychis viciifolia (sainfoin), Lespedeza capitata (roundhead lespedeza), and Galega officinalis (goat's rue). The phytochemical complexes of these species contain secondary metabolites whose pharmacological potentials deserve investigation. Major classes of compounds include alkaloids and amines, cyanogenic glycosides, flavonoids, coumarins, condensed tannins, and saponins. Some of these phytochemicals have been related to antihypercholesterolemia, antidiabetic, antimenopause, anti-inflammatory, antiedema, anthelmintic, and kidney protective effects. Two widely prescribed drugs have been developed starting from temperate forage legumes, namely, the antithrombotic warfarin, inspired from sweet clover's coumarin, and the antidiabetic metformin, a derivative of sainfoin's guanidine. Available evidence suggests that temperate forage legumes are a potentially important resource for the extraction of active principles to be used as nutraceuticals and pharmaceuticals.
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therapeutic potential of temperate forage legumes a review
Critical Reviews in Food Science and Nutrition, 2016Co-Authors: Laura Cornara, Jianbo Xiao, Bruno BurlandoAbstract:The discovery of bioactive molecules from botanical sources is an expanding field, preferentially oriented to plants having a tradition of use in medicine and providing high yields and availability. Temperate forage legumes are Fabaceae species that include worldwide-important crops. These plants possess therapeutic virtues that have not only been used in veterinary and folk medicine, but have also attracted the interest of official medicine. We have examined here Medicago sativa (alfalfa), Trifolium pratense and T. repens (clovers), Melilotus albus and M. officinalis (sweet clovers), Lotus corniculatus (birdsfoot trefoil), Onobrychis viciifolia (sainfoin), Lespedeza capitata (roundhead lespedeza), and Galega officinalis (goat's rue). The phytochemical complexes of these species contain secondary metabolites whose pharmacological potentials deserve investigation. Major classes of compounds include alkaloids and amines, cyanogenic glycosides, flavonoids, coumarins, condensed tannins, and saponins. Some of ...
Natalia Sybirna - One of the best experts on this subject based on the ideXlab platform.
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medicinal plants Galega officinalis l and yacon leaves as potential sources of antidiabetic drugs
Antioxidants, 2021Co-Authors: Halyna Hachkova, Mariia Nagalievska, Zoriana Soliljak, Olena Kanyuka, Alicja Z Kucharska, Anna Sokolłetowska, Elena Belonovskaya, Vyacheslav Buko, Natalia SybirnaAbstract:Hypoglycemic and antioxidant properties of extracts of medicinal plants Galega officinalis L. (aboveground part) and yacon (Smallanthus sonchifolius Poepp. & Endl.) (leaves) as potential sources of biologically active substances with antidiabetic action have been studied. The pronounced hypoglycemic effect of Galega officinalis extract, devoid of alkaloids, at a dose of 600 mg/kg in experimental diabetes mellitus (DM) has been proven. The established effect is evidenced by a decrease in the concentration of glucose and glycosylated hemoglobin in the blood, increase glucose tolerance of cells, increase C-peptide and insulin content in the plasma of rats’ blood. The effective hypoglycemic effect of the extract in the studied pathology was confirmed by histological examination of the pancreas. The cytoprotective effect of the studied extract on pancreatic cells at a dose of 1200 mg/kg was experimentally confirmed. In the standard cut area, an increase was found in the number of Langerhans islets, their average area, diameter, volume, and a number of β-cells relative to these indicators in animals with diabetes. Comparative screening of the antioxidant properties of 30, 50, 70, and 96% water–ethanol extracts of yacon indicates the highest potential of 50% water-ethanol extract to block free radicals in in vitro model experiments. The non-alkaloid fraction of Galega officinalis extract showed moderate antioxidant activity and was inferior to yacon extract in its ability to neutralize reactive oxygen species (ROS) and bind metal ions of variable valence. The level of antioxidant potential of the studied extracts is due to differences in the quantitative content of compounds of phenolic nature in their compositions. The obtained data on the biological effects of Galega officinalis extract on the structural and functional state of β-cells of the pancreas and antioxidant properties of Galega officinalis and yacon extracts substantiate the prospects of using these plants to create antidiabetic medicines and functional foods based on them.
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Galega officinalis extract regulate the diabetes mellitus related violations of proliferation functions and apoptosis of leukocytes
BMC Complementary and Alternative Medicine, 2018Co-Authors: Mariia Nagalievska, Halyna Hachkova, Mariya Sabadashka, Natalia SybirnaAbstract:An impaired leukocytes function is the factor causing the susceptibility of patients with diabetes mellitus to infections. The outmost importance for the understanding of the immunological processes involved in diabetes pathogenesis is to give the characteritics of the immunological profile and changes therein, during the course of desease. Long-used in folk medicine to treat diabetes Galega officinalis L. has been chosen for the correction of the immune system dysfunction. The experiments were conducted on male Wistar rats. Fractionation of bone marrow cells suspension was performed in a three-layer ficoll–sodium amidotrizoate density gradient. The lymphocytic-granulocytic cells proliferative activity was studied using enzyme immunoassay with 5-bromo-2′-deoxyuridine (BrdU). For staining of bone marrow preparations May-Gruenwald-Romanowsky-Giemsa (Pappenheim) method was used. To evaluate the content of cationic proteins and myeloperoxidase in neutrophilic leukocytes cytochemical studies were performed. Content of tumor necrosis factor alpha was carried out by immuno-enzymatic analysis. Lymphocytes apoptosis was examined by fluorescent analysis using annexin V. Diabetes mellitus development was accompanied with violation of neutrophils and lymphocytes proliferation, increased activity of myeloperoxidase and enhanced apoptosis process. Administration of Galega officinalis extract under the condition of diabetes promotes the restoration of neutrophils bone marrow pool and the reduction of lymphoblasts number and causes inhibition of the lymphocytes apoptosis process. Investigated medicine has a pronounced immunocorrective effect under the conditions of diabetes mellitus and can become the basis for creating a new generation of antidiabetic drugs.
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Leukocyte actin cytoskeleton reorganization and redistribution of sialylated membrane glycoconjugates under experimental diabetes mellitus and against the administration of the Galega officinalis L. extract
Cytology and Genetics, 2017Co-Authors: M. Lupak, H. Hachkova, M. Khokhla, Ya. Chajka, M. Skybitska, Natalia SybirnaAbstract:The article describes the effect of alkaloid-free fraction of the Galega officinalis extract (AFFGE) on the aggregation ability of immunocompetent blood cells, as well as on the process of actin polymerization and structural rearrangements among sialylated glycoconjugates of the peripheral blood leukocyte membranes of rats in the norm and under experimental diabetes mellitus (EDM) conditions. The flow cytometry method (using phalloidin labelled with fluorescent tetramethyl rhodamine-5-isothiocyanate (TRITC)) and the western blot analysis have allowed us to detect an increase in the rat leukocyte F-actin content in the event of diabetes mellitus, which indicated changes in the structural and functional properties of the leukocytes and their preactivation phase. A quantitative analysis of the total polymerized actin pool redistribution between its constituent fraction (represented by cytoskeletal filaments) and short actin filaments has shown that, against an increase in the total F-actin level, the number of actin filaments of the cytoskeleton decreased and the content of short actin filaments increased in leukocytes of animals with EDM. The use of sialylated lectins has allowed a conclusion to be made on the study of the pathology that the number of exposed oligosaccharide determinants on leukocyte membrane, the structure of which contained N-acetyl-β-D-glucosamine and sialic acid residues, increased, whereas the number of sialic acid-containing surface glycoconjugates bound to subterminal galactose residues by α2→3 and α2→6-glycoside bonds decreased. The administration of AFFGE to diabetic animals led to an increase in the content of F-actin and short filaments of the leukocyte cytoskeleton and a reduction in the lectin-induced leukocyte aggregation. The correction effect of the studied extract on the functional state of leukocytes can be realized through the action on the processes underlying the formation of the actin cytoskeletal elements and due to the quantitative redistribution of leukocyte membrane glycoconjugates with different structures of carbohydrate determinants, such as, due to a decrease in the exposure of N-acetyl-β-D-glucosamine residues and an increase in the exposure of sialic acids bound to subterminal galactose residues by α2→3 and α2→6-glycoside bonds.
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Application of biogenic surfactants for stabilization on alkaloid-free fraction isolated from Galega officinalis extract
Львівський національний університет імені Івана Франка, 2015Co-Authors: M. Lupak, M. Khokhla, G. Hachkova, O. Shulga, N. Sheglova, R. Vildanova, А. Zyn, Natalia SybirnaAbstract:The article contains data on the influence of biogenic surfactants synthesized by bacteria Pseudomonas sp. PS-17 on the stability of emulsions based on alkaloid-free fraction isolated from Galega officinalis L. extract. The optimum concentrations of biogenic surfactants to stabilize investigated emulsionswere experimentally chosen. The chemical composition of alkaloid-free fractions from Galega officinalis L. extract before and after stabilization were investigated. The resulting substance is easier to administer to animals through a tube, which not only improves the accuracy of the dosage, but also increases its bioavailability. Reduction of glycosylated hemoglobin at 13.4 % and glucose content to the physiological values in the rats blood with experimental diabetes mellitus by administration within 14 days stabilized emulsions compared with the original emulsion shows that emulsion stabilized by the biogenic surfactant based on alkaloid-free fraction isolated from Galega officinalis an extract provides effective hyperglycemia compensation in rats under experimental diabetes mellitus, compared with the original mixture, which may be caused by changes in the proportion of biologically active substances in the extract while stabilizing and its better bioavailability
Laura Cornara - One of the best experts on this subject based on the ideXlab platform.
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Therapeutic Potential of Temperate Forage Legumes: A Review.
Critical reviews in food science and nutrition, 2016Co-Authors: Laura Cornara, Jianbo Xiao, Bruno BurlandoAbstract:The discovery of bioactive molecules from botanical sources is an expanding field, preferentially oriented to plants having a tradition of use in medicine and providing high yields and availability. Temperate forage legumes are Fabaceae species that include worldwide-important crops. These plants possess therapeutic virtues that have not only been used in veterinary and folk medicine, but have also attracted the interest of official medicine. We have examined here Medicago sativa (alfalfa), Trifolium pratense and T. repens (clovers), Melilotus albus and M. officinalis (sweet clovers), Lotus corniculatus (birdsfoot trefoil), Onobrychis viciifolia (sainfoin), Lespedeza capitata (roundhead lespedeza), and Galega officinalis (goat's rue). The phytochemical complexes of these species contain secondary metabolites whose pharmacological potentials deserve investigation. Major classes of compounds include alkaloids and amines, cyanogenic glycosides, flavonoids, coumarins, condensed tannins, and saponins. Some of these phytochemicals have been related to antihypercholesterolemia, antidiabetic, antimenopause, anti-inflammatory, antiedema, anthelmintic, and kidney protective effects. Two widely prescribed drugs have been developed starting from temperate forage legumes, namely, the antithrombotic warfarin, inspired from sweet clover's coumarin, and the antidiabetic metformin, a derivative of sainfoin's guanidine. Available evidence suggests that temperate forage legumes are a potentially important resource for the extraction of active principles to be used as nutraceuticals and pharmaceuticals.
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therapeutic potential of temperate forage legumes a review
Critical Reviews in Food Science and Nutrition, 2016Co-Authors: Laura Cornara, Jianbo Xiao, Bruno BurlandoAbstract:The discovery of bioactive molecules from botanical sources is an expanding field, preferentially oriented to plants having a tradition of use in medicine and providing high yields and availability. Temperate forage legumes are Fabaceae species that include worldwide-important crops. These plants possess therapeutic virtues that have not only been used in veterinary and folk medicine, but have also attracted the interest of official medicine. We have examined here Medicago sativa (alfalfa), Trifolium pratense and T. repens (clovers), Melilotus albus and M. officinalis (sweet clovers), Lotus corniculatus (birdsfoot trefoil), Onobrychis viciifolia (sainfoin), Lespedeza capitata (roundhead lespedeza), and Galega officinalis (goat's rue). The phytochemical complexes of these species contain secondary metabolites whose pharmacological potentials deserve investigation. Major classes of compounds include alkaloids and amines, cyanogenic glycosides, flavonoids, coumarins, condensed tannins, and saponins. Some of ...
