The Experts below are selected from a list of 4641 Experts worldwide ranked by ideXlab platform
Min Yan - One of the best experts on this subject based on the ideXlab platform.
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Genetic Purity testing of pumpkin hybrid seed by ultrathin-layer isoelectric focusing
Crop Breeding and Applied Biotechnology, 2013Co-Authors: Min YanAbstract:Ultrathin-layer isoelectric focusing of seed proteins was used to test the Genetic Purity of pumpkin hybrid seeds. In the pH range of 5-8, one male marker band and two female marker bands were found in the male and female parental line, respectively. Based on the male marker band and female marker bands, the Genetic Purity of F1 seeds can easily be determined in the laboratory.
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logo Genetic Purity testing of two-line hybrid rice seeds by ultrathin-layer isoelectric focusing of proteins
Seed Science and Technology, 2005Co-Authors: T. Zhao, Min Yan, F. Yang, J. Huang, Xiaofeng WangAbstract:To test for inbreds among F1 seeds of two-line hybrid rice (Oryza sativa L.) in the laboratory, ultrathin-layer isoelectric focusing (UTLIEF) of seed proteins was used. One female marker band in the photo-thermo-sensitive genic male sterile rice (cv. Peiai 64S) and one common male marker band in the four restorer lines (cv. G67, Peifu, I9-1 and Minkezhan) were found in the pH range of 5-8. Embryoless half-seeds contained the same male marker proteins as whole seeds and could be used for UTLIEF to test inbreds in the laboratory. The corresponding embryo-containing half-seeds could germinate to form seedlings on vermiculite and seedlings could be transplanted into the field for grow-out tests. The Genetic Purity determined by UTLIEF of proteins from embryoless half-seeds was confirmed by grow-out tests of the corresponding embryo-containing half-seeds. This work shows that UTLIEF of seed proteins could replace grow-out tests to determine F1 Genetic Purity of two-line hybrid rice. Using half-seeds, the comparison between laboratory and field tests was more objective and accurate than using whole seeds.
Igor V. Aksyonov - One of the best experts on this subject based on the ideXlab platform.
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USE OF ALBUMIN MARKERS FOR DEFINING Genetic Purity OF SUNFLOWER PARENT LINES AND HYBRIDS / UTILIZACIÓN DE MARCADORES ALBUMÍNICOS EN DEFINICIÓN DE PUREZA GENÉTICA DE LAS LÍNEAS PROGENITORAS E HÍBRIDOS DE GIRASOL / L’UTILISATION DE MARQUEURS ALBUMINEUX
Helia, 2005Co-Authors: Igor V. AksyonovAbstract:Experiments have been conducted at Institute of Oilseed Crops, UAAS, Ukraine, to study the usefulness of electrophoresis of seed storage proteins in defining Genetic Purity of sunflower seed material. They have shown it is possible to use albumin markers for Genetic homogeneity definition of sunflower parent lines and hybrids.
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use of albumin markers for defining Genetic Purity of sunflower parent lines and hybrids utilizacion de marcadores albuminicos en definicion de pureza Genetica de las lineas progenitoras e hibridos de girasol l utilisation de marqueurs albumineux dan
Helia, 2005Co-Authors: Igor V. AksyonovAbstract:Experiments have been conducted at Institute of Oilseed Crops, UAAS, Ukraine, to study the usefulness of electrophoresis of seed storage proteins in defining Genetic Purity of sunflower seed material. They have shown it is possible to use albumin markers for Genetic homogeneity definition of sunflower parent lines and hybrids.
Zhao Yan-ming - One of the best experts on this subject based on the ideXlab platform.
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Comparison of Two Methods Used to Test the Genetic Purity of Hybrid Rice Seeds
2008Co-Authors: Zhao Yan-mingAbstract:Ultrathin-layer isoelectric focusing of seed proteins(UTLIEF) and grow-out test were used to test the Genetic Purity of hybrid rice seeds.The results showed that Genetic Purity determined by UTLIEF was the same as that of grow-out test and UTLIEF cost less money and fewer hours than grow-out test.
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Genetic Purity Testing of Seed from Two-line Hybrid Rice(Oryza spp.) of Variety Peizaminkezhan
2008Co-Authors: Zhao Yan-mingAbstract:Ultrathin-layer isoelectric focusing of seed proteins(UTLIEF)test were used to test the Genetic Purity of hybrid rice seeds.Results showed:Genetic Purity determined by UTLIEF was the same as that of growout test and UTLIEF cost less money and fewer hours than grow-out test.
Yiqin Gong - One of the best experts on this subject based on the ideXlab platform.
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Genetic Purity testing of f1 hybrid seed with molecular markers in cabbage brassica oleracea var capitata
Scientia Horticulturae, 2013Co-Authors: Shan Ye, Danqiong Huang, Yiqin Gong, Jianbin Li, Yan Wang, Liang XuAbstract:Abstract The seed Genetic Purity of cabbage F1 hybrid cultivar, ‘Sugan 21’, was analyzed with PCR-based molecular markers including SSR, RAPD, ISSR and SRAP in this study. Among the total of 325 primers including 85 SSR, 80 RAPD, 96 ISSR and 64 SRAP screened, only seven primers could produce both female parent-specific (FPS) and male parent-specific (MPS) markers. A total of 216 F1 hybrid individuals were genotyped with these seven primers belonging to three molecular marker systems. It was found that four out of the 216 F1 individuals were false hybrids, among which three could only generate FPS markers and the rest generated MPS markers only. Therefore, the Genetic Purity of ‘Sugan 21’ seed lot used in this study was calculated as 98.15%. Grow-out-trials (GOTs) were carried out to test the seed Genetic Purity and validate the authenticity of the molecular markers analysis, and it was found that five out of the 216 F1 plants were false hybrids and the seed Purity from GOTs was 97.69%. These results indicated that the molecular markers could be used as a more rapid, practical and efficient tool in quality control of the cabbage commercial hybrid seeds.
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Genetic Purity testing of F1 hybrid seed with molecular markers in cabbage (Brassica oleracea var. capitata)
Scientia Horticulturae, 2013Co-Authors: Yan Wang, Danqiong Huang, Yiqin Gong, Liwang LiuAbstract:a b s t r a c t The seed Genetic Purity of cabbage F1 hybrid cultivar, 'Sugan 21', was analyzed with PCR-based molecular markers including SSR, RAPD, ISSR and SRAP in this study. Among the total of 325 primers including 85 SSR, 80 RAPD, 96 ISSR and 64 SRAP screened, only seven primers could produce both female parent-specific (FPS) and male parent-specific (MPS) markers. A total of 216 F1 hybrid individuals were genotyped with these seven primers belonging to three molecular marker systems. It was found that four out of the 216 F1 individuals were false hybrids, among which three could only generate FPS markers and the rest generated MPS markers only. Therefore, the Genetic Purity of 'Sugan 21' seed lot used in this study was calculated as 98.15%. Grow-out-trials (GOTs) were carried out to test the seed Genetic Purity and validate the authenticity of the molecular markers analysis, and it was found that five out of the 216 F1 plants were false hybrids and the seed Purity from GOTs was 97.69%. These results indicated that the molecular markers could be used as a more rapid, practical and efficient tool in quality control of the cabbage commercial hybrid seeds. © 2013 Elsevier B.V. All rights reserved.
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Assessment of Genetic Purity of tomato (Lycopersicon esculentum L.) hybrid using molecular markers
Scientia Horticulturae, 2007Co-Authors: Liwang Liu, Yiqin Gong, Yan Wang, Tong-min Zhao, Guang LiuAbstract:Abstract Three DNA molecular marker systems, RAPD, ISSR and SSR, were used to test seed Genetic Purity of two commercial hybrid tomato ( Lycopersicon esculentum L.) cultivars ‘Hezuo 903’ and ‘Sufen No. 8’. Genomic DNA from the two F 1 hybrid cultivars and their corresponding parental lines was screened with 218 RAPD decamer primers, 54 ISSR primers and 49 SSR primers. Among the 321 primers, 4 primers for ‘Hezuo 903’ and 3 for ‘Sufen No. 8’, which could produce both female and male parent-specific markers, were selected for testing the Genetic Purity. A total of 210 hybrid individuals of each cultivar were analyzed using the identified primers. The combined results of the marker analysis showed that eight of the 210 F 1 plants in ‘Hezuo 903’ and 13 of 210 in ‘Sufen No. 8’ were false hybrids, and the overall Genetic Purity of the two F 1 hybrid seed lots was 96.2 and 93.8%, respectively. This study showed that RAPD and SSR markers could provide a practical and efficient tool in quality control of the tomato commercial hybrid seeds.
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evaluation of Genetic Purity of f1 hybrid seeds in cabbage with rapd issr srap and ssr markers
Hortscience, 2007Co-Authors: Yiqin Gong, Yan Wang, Fanmin YuAbstract:Additional index words. Brassica oleracea var. capitata, 'Zaoxia 16' (F1), codominant marker Abstract. Four molecular marker systems—RAPD (random amplified polymorphic DNA), ISSR (intersimple sequence repeat), SRAP (sequence-related amplified poly- morphism), and SSR (simple sequence repeat)—were used to evaluate seed Genetic Purity of a hybrid cabbage cultivar 'Zaoxia 16'. Genetic relationships of the F1 hybrids and their parents were analyzed with 157 RAPD primers, 54 ISSR primers, 84 SRAP primer combinations, and 44 SSR primers. Three RAPD primers (NAURP2006, NAURP2020, and NAURP2031), two ISSR primers (NAUISR1058 and NAUISR1062), one SRAP primer combination (NAUSR04/NAURS05), and two SSR primers (NAUSSR1011 and NAUSSR1031), which produced male and female parent-specific markers simulta- neously, were selected for testing the Genetic Purity of the F1 seeds. A total of 210 'Zaoxia 16' hybrid individuals were investigated with these eight selected primers. Of these, 12 appeared to be false hybrids. Nine of the 12 putative false hybrids, confirmed with all eight primers, exhibited similar banding patterns to the female parent, suggesting that they could be derived from selfing of the female parent. The results were in accordance with those from field evaluations. This study showed that RAPD, ISSR, SRAP, and SSR markers are highly efficient and reproducible for Genetic Purity testing of cabbage commercial hybrid seeds.
Yan Wang - One of the best experts on this subject based on the ideXlab platform.
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Genetic Purity testing of f1 hybrid seed with molecular markers in cabbage brassica oleracea var capitata
Scientia Horticulturae, 2013Co-Authors: Shan Ye, Danqiong Huang, Yiqin Gong, Jianbin Li, Yan Wang, Liang XuAbstract:Abstract The seed Genetic Purity of cabbage F1 hybrid cultivar, ‘Sugan 21’, was analyzed with PCR-based molecular markers including SSR, RAPD, ISSR and SRAP in this study. Among the total of 325 primers including 85 SSR, 80 RAPD, 96 ISSR and 64 SRAP screened, only seven primers could produce both female parent-specific (FPS) and male parent-specific (MPS) markers. A total of 216 F1 hybrid individuals were genotyped with these seven primers belonging to three molecular marker systems. It was found that four out of the 216 F1 individuals were false hybrids, among which three could only generate FPS markers and the rest generated MPS markers only. Therefore, the Genetic Purity of ‘Sugan 21’ seed lot used in this study was calculated as 98.15%. Grow-out-trials (GOTs) were carried out to test the seed Genetic Purity and validate the authenticity of the molecular markers analysis, and it was found that five out of the 216 F1 plants were false hybrids and the seed Purity from GOTs was 97.69%. These results indicated that the molecular markers could be used as a more rapid, practical and efficient tool in quality control of the cabbage commercial hybrid seeds.
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Genetic Purity testing of F1 hybrid seed with molecular markers in cabbage (Brassica oleracea var. capitata)
Scientia Horticulturae, 2013Co-Authors: Yan Wang, Danqiong Huang, Yiqin Gong, Liwang LiuAbstract:a b s t r a c t The seed Genetic Purity of cabbage F1 hybrid cultivar, 'Sugan 21', was analyzed with PCR-based molecular markers including SSR, RAPD, ISSR and SRAP in this study. Among the total of 325 primers including 85 SSR, 80 RAPD, 96 ISSR and 64 SRAP screened, only seven primers could produce both female parent-specific (FPS) and male parent-specific (MPS) markers. A total of 216 F1 hybrid individuals were genotyped with these seven primers belonging to three molecular marker systems. It was found that four out of the 216 F1 individuals were false hybrids, among which three could only generate FPS markers and the rest generated MPS markers only. Therefore, the Genetic Purity of 'Sugan 21' seed lot used in this study was calculated as 98.15%. Grow-out-trials (GOTs) were carried out to test the seed Genetic Purity and validate the authenticity of the molecular markers analysis, and it was found that five out of the 216 F1 plants were false hybrids and the seed Purity from GOTs was 97.69%. These results indicated that the molecular markers could be used as a more rapid, practical and efficient tool in quality control of the cabbage commercial hybrid seeds. © 2013 Elsevier B.V. All rights reserved.
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Assessment of Genetic Purity of tomato (Lycopersicon esculentum L.) hybrid using molecular markers
Scientia Horticulturae, 2007Co-Authors: Liwang Liu, Yiqin Gong, Yan Wang, Tong-min Zhao, Guang LiuAbstract:Abstract Three DNA molecular marker systems, RAPD, ISSR and SSR, were used to test seed Genetic Purity of two commercial hybrid tomato ( Lycopersicon esculentum L.) cultivars ‘Hezuo 903’ and ‘Sufen No. 8’. Genomic DNA from the two F 1 hybrid cultivars and their corresponding parental lines was screened with 218 RAPD decamer primers, 54 ISSR primers and 49 SSR primers. Among the 321 primers, 4 primers for ‘Hezuo 903’ and 3 for ‘Sufen No. 8’, which could produce both female and male parent-specific markers, were selected for testing the Genetic Purity. A total of 210 hybrid individuals of each cultivar were analyzed using the identified primers. The combined results of the marker analysis showed that eight of the 210 F 1 plants in ‘Hezuo 903’ and 13 of 210 in ‘Sufen No. 8’ were false hybrids, and the overall Genetic Purity of the two F 1 hybrid seed lots was 96.2 and 93.8%, respectively. This study showed that RAPD and SSR markers could provide a practical and efficient tool in quality control of the tomato commercial hybrid seeds.
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evaluation of Genetic Purity of f1 hybrid seeds in cabbage with rapd issr srap and ssr markers
Hortscience, 2007Co-Authors: Yiqin Gong, Yan Wang, Fanmin YuAbstract:Additional index words. Brassica oleracea var. capitata, 'Zaoxia 16' (F1), codominant marker Abstract. Four molecular marker systems—RAPD (random amplified polymorphic DNA), ISSR (intersimple sequence repeat), SRAP (sequence-related amplified poly- morphism), and SSR (simple sequence repeat)—were used to evaluate seed Genetic Purity of a hybrid cabbage cultivar 'Zaoxia 16'. Genetic relationships of the F1 hybrids and their parents were analyzed with 157 RAPD primers, 54 ISSR primers, 84 SRAP primer combinations, and 44 SSR primers. Three RAPD primers (NAURP2006, NAURP2020, and NAURP2031), two ISSR primers (NAUISR1058 and NAUISR1062), one SRAP primer combination (NAUSR04/NAURS05), and two SSR primers (NAUSSR1011 and NAUSSR1031), which produced male and female parent-specific markers simulta- neously, were selected for testing the Genetic Purity of the F1 seeds. A total of 210 'Zaoxia 16' hybrid individuals were investigated with these eight selected primers. Of these, 12 appeared to be false hybrids. Nine of the 12 putative false hybrids, confirmed with all eight primers, exhibited similar banding patterns to the female parent, suggesting that they could be derived from selfing of the female parent. The results were in accordance with those from field evaluations. This study showed that RAPD, ISSR, SRAP, and SSR markers are highly efficient and reproducible for Genetic Purity testing of cabbage commercial hybrid seeds.