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Nuria Ferrol - One of the best experts on this subject based on the ideXlab platform.
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RESEARCH ARTICLE Characterization of a CuZn superoxide dismutase gene in the arbuscular mycorrhizal fungus Glomus Intraradices
2016Co-Authors: Elodie Oger, Karim Benabdellah, Luisa Lanfranco, Nuria FerrolAbstract:Abstract To gain further insights into the mechanisms of redox homeostasis in arbuscular mycorrhizal fungi, we characterized a Glomus Intraradices gene (GintSOD1) showing high similarity to previously described genes encoding CuZn superoxide dismutases (SODs). The Gint-SOD1 gene consists of an open reading frame of 471 bp, predicted to encode a protein of 157 amino acids with an estimated molecular mass of 16.3 kDa. Functional com-plementation assays in a CuZnSOD-defective yeast mutant showed that GintSOD1 protects the yeast cells from oxy-gen toxicity and that it, therefore, encodes a protein that scavenges reactive oxygen species (ROS). GintSOD1 transcripts differentially accumulate during the fungal life cycle, reaching the highest expression levels in the intra-radical mycelium. GintSOD1 expression is induced by the well known ROS-inducing agents paraquat and copper, and also by fenpropimorph, a sterol biosynthesis inhibitor (SBI) fungicide. These results suggest that GintSOD1 is involved in the detoxification of ROS generated from metabolic processes and by external agents. In particular, our data indicate that the antifungal effects of fenpropimorph might not be only due to the interference with sterol metabolism but also to the perturbation of other biological processes and that ROS production and scavenging systems are involved in the response to SBI fungicides
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gintamt2 a new member of the ammonium transporter family in the arbuscular mycorrhizal fungus Glomus Intraradices
Fungal Genetics and Biology, 2011Co-Authors: Jacob Pereztienda, Concepcion Azconaguilar, P S Testillano, Raffaella Balestrini, Valentina Fiorilli, Nuria FerrolAbstract:In the symbiotic association of plants and arbuscular mycorrhizal (AM) fungi, the fungus delivers mineral nutrients, such as phosphate and nitrogen, to the plant while receiving carbon. Previously, we identified an NH(4)(+) transporter in the AM fungus Glomus Intraradices (GintAMT1) involved in NH(4)(+) uptake from the soil when preset at low concentrations. Here, we report the isolation and characterization of a new G. IntraradicesNH(4)(+) transporter gene (GintAMT2). Yeast mutant complementation assays showed that GintAMT2 encodes a functional NH(4)(+) transporter. The use of an anti-GintAMT2 polyclonal antibody revealed a plasma membrane location of GintAMT2. GintAMT1 and GintAMT2 were differentially expressed during the fungal life cycle and in response to N. In contrast to GintAMT1, GintAMT2 transcript levels were higher in the intraradical than in the extraradical fungal structures. However, transcripts of both genes were detected in arbuscule-colonized cortical cells. GintAMT1 expression was induced under low N conditions. Constitutive expression of GintAMT2 in N-limiting conditions and transitory induction after N re-supply suggests a role for GintAMT2 to retrieve NH(4)(+) leaked out during fungal metabolism.
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Characterization of a CuZn superoxide dismutase gene in the arbuscular mycorrhizal fungus Glomus Intraradices
Current Genetics, 2010Co-Authors: Manuel González-guerrero, Elodie Oger, Karim Benabdellah, Concepción Azcón-aguilar, Luisa Lanfranco, Nuria FerrolAbstract:To gain further insights into the mechanisms of redox homeostasis in arbuscular mycorrhizal fungi, we characterized a Glomus Intraradices gene ( GintSOD1 ) showing high similarity to previously described genes encoding CuZn superoxide dismutases (SODs). The GintSOD1 gene consists of an open reading frame of 471 bp, predicted to encode a protein of 157 amino acids with an estimated molecular mass of 16.3 kDa. Functional complementation assays in a CuZnSOD -defective yeast mutant showed that GintSOD1 protects the yeast cells from oxygen toxicity and that it, therefore, encodes a protein that scavenges reactive oxygen species (ROS). GintSOD1 transcripts differentially accumulate during the fungal life cycle, reaching the highest expression levels in the intraradical mycelium. GintSOD1 expression is induced by the well known ROS-inducing agents paraquat and copper, and also by fenpropimorph, a sterol biosynthesis inhibitor (SBI) fungicide. These results suggest that GintSOD1 is involved in the detoxification of ROS generated from metabolic processes and by external agents. In particular, our data indicate that the antifungal effects of fenpropimorph might not be only due to the interference with sterol metabolism but also to the perturbation of other biological processes and that ROS production and scavenging systems are involved in the response to SBI fungicides.
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GintABC1 encodes a putative ABC transporter of the MRP subfamily induced by Cu, Cd, and oxidative stress in Glomus Intraradices
Mycorrhiza, 2010Co-Authors: Manuel González-guerrero, Ascension Valderas, Karim Benabdellah, Concepción Azcón-aguilar, Nuria FerrolAbstract:A full-length cDNA sequence putatively encoding an ATP-binding cassette (ABC) transporter ( GintABC1 ) was isolated from the extraradical mycelia of the arbuscular mycorrhizal fungus Glomus Intraradices . Bioinformatic analysis of the sequence indicated that GintABC1 encodes a 1513 amino acid polypeptide, containing two six-transmembrane clusters (TMD) intercalated with sequences characteristics of the nucleotide binding domains (NBD) and an extra N-terminus extension (TMD0). GintABC1 presents a predicted TMD0-(TMD-NBD)_2 topology, typical of the multidrug resistance-associated protein subfamily of ABC transporters. Gene expression analyses revealed no difference in the expression levels of GintABC1 in the extra- vs the intraradical mycelia. GintABC1 was up-regulated by Cd and Cu, but not by Zn, suggesting that this transporter might be involved in Cu and Cd detoxification. Paraquat, an oxidative agent, also induced the transcription of GintABC1 . These data suggest that redox changes may be involved in the transcriptional regulation of GintABC1 by Cd and Cu.
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ultrastructural localization of heavy metals in the extraradical mycelium and spores of the arbuscular mycorrhizal fungus Glomus Intraradices
Canadian Journal of Microbiology, 2008Co-Authors: Manuel Gonzalezguerrero, Nuria Ferrol, Lewis Melvillel H H Melville, John N A LottAbstract:Arbuscular mycorrhizal fungi, obligate symbionts of most plant species, are able to accumulate heavy metals, thereby, protecting plants from metal toxicity. In this study, the ultrastructural localization of Zn, Cu, and Cd in the extraradical mycelium and spores of the arbuscular mycorrhizal fungus Glomus Intraradices grown in monoxenic cultures was investigated. Zinc, Cu, or Cd was applied to the extraradical mycelium to final concentrations of 7.5, 5.0, or 0.45 mmol/L, respectively. Samples were collected at time 0, 8 h, and 7 days after metal application and were prepared for rapid freezing and freeze substitution. Metal content in different subcellular locations (wall, cytoplasm, and vacuoles), both in hyphae and spores, was determined by energy-dispersive X-ray spectroscopy. In all treatments and fungal structures analysed, heavy metals accumulated mainly in the fungal cell wall and in the vacuoles, while minor changes in metal concentrations were detected in the cytoplasm. Incorporation of Zn into t...
Alicia Margarita Godeas - One of the best experts on this subject based on the ideXlab platform.
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flavonoids from shoots and roots of trifolium repens white clover grown in presence or absence of the arbuscular mycorrhizal fungus Glomus Intraradices
Phytochemistry, 2004Co-Authors: Maria A Ponce, J A Ocampo, Jose M Scervino, Rosa Errabalsells, Alicia Margarita GodeasAbstract:White clover (Trifolium repens) plants were grown in the presence or absence of the arbuscular mycorrhizal fungus Glomus Intraradices. Flavones, 4 0 ,5,6,7,8-pentahydroxy-3-methoxyflavone and 5,6,7,8-tetrahydroxy-3-methoxyflavone, as well as two flavones 3,7-dihydroxy-4 0 -methoxyflavone and 5,6,7,8-tetrahydroxy-4 0 -methoxyflavone never previously reported in plants, were isolated. The known 3,5,6,7,8-pentahydroxy-4 0 -methoxyflavone, 2 0 ,3 0 ,4 0 ,5 0 ,6 0 -pentahydroxy-chalcone, 6-hydroxykaempferol, 4 0 ,5,6,7,8-pentahydroxyflavone and 3,4 0 -dimethoxykaempferol were also obtained. Analysis of extracts obtained from roots and shoots revealed that the compositions of the flavonoid mixtures varied with growing conditions. Quercetin, acacetin and rhamnetin accumulated in roots of inoculated plants, whereas they were not detected in non-inoculated plants. 2004 Published by Elsevier Ltd.
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flavonoids from shoots and roots of trifolium repens white clover grown in presence or absence of the arbuscular mycorrhizal fungus Glomus Intraradices
Phytochemistry, 2004Co-Authors: Maria A Ponce, J A Ocampo, Jose M Scervino, Rosa Errabalsells, Alicia Margarita GodeasAbstract:White clover (Trifolium repens) plants were grown in the presence or absence of the arbuscular mycorrhizal fungus Glomus Intraradices. Flavones, 4',5,6,7,8-pentahydroxy-3-methoxyflavone and 5,6,7,8-tetrahydroxy-3-methoxyflavone, as well as two flavones 3,7-dihydroxy-4'-methoxyflavone and 5,6,7,8-tetrahydroxy-4'-methoxyflavone never previously reported in plants, were isolated. The known 3,5,6,7,8-pentahydroxy-4'-methoxyflavone, 2',3',4',5',6'-pentahydroxy-chalcone, 6-hydroxykaempferol, 4',5,6,7,8-pentahydroxyflavone and 3,4'-dimethoxykaempferol were also obtained. Analysis of extracts obtained from roots and shoots revealed that the compositions of the flavonoid mixtures varied with growing conditions. Quercetin, acacetin and rhamnetin accumulated in roots of inoculated plants, whereas they were not detected in non-inoculated plants.
Marcelo E. Doucet - One of the best experts on this subject based on the ideXlab platform.
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Effect of the arbuscular mycorrhizal fungus Glomus Intraradices on the false root-knot nematode Nacobbus aberrans in tomato plants
Biology and Fertility of Soils, 2011Co-Authors: Paola Lax, Alejandra G. Becerra, Florencia Soteras, Marta Cabello, Marcelo E. DoucetAbstract:Information about the interaction between arbuscular mycorrhizal fungi (AMF) and the false root-knot nematode Nacobbus aberrans (Thorne, 1935) Thorne & Allen, 1944 is scarce. The effect of Glomus Intraradices Schenk & Smith on tomato ( Lycopersicon esculentum L.) cv. Platense inoculated with nematode juveniles from Lisandro Olmos (Argentina) was studied under greenhouse conditions. Six treatments with five replications were performed. After 80 days, nematode reproduction and percentage of AMF colonization in roots were estimated. Some plant growth parameters were also measured. In general, plants with AMF and AMF plus nematodes grew as well as the control without AMF and without nematodes. Furthermore, G. Intraradices was beneficial in reducing nematode-induced damage in roots (lower number of galls) as well as in having a suppressive effect on parasite reproduction. This is the first study on the use of G. Intraradices as a possible strategy in the control of N. aberrans in tomato.
Maria A Ponce - One of the best experts on this subject based on the ideXlab platform.
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flavonoids from shoots and roots of trifolium repens white clover grown in presence or absence of the arbuscular mycorrhizal fungus Glomus Intraradices
Phytochemistry, 2004Co-Authors: Maria A Ponce, J A Ocampo, Jose M Scervino, Rosa Errabalsells, Alicia Margarita GodeasAbstract:White clover (Trifolium repens) plants were grown in the presence or absence of the arbuscular mycorrhizal fungus Glomus Intraradices. Flavones, 4 0 ,5,6,7,8-pentahydroxy-3-methoxyflavone and 5,6,7,8-tetrahydroxy-3-methoxyflavone, as well as two flavones 3,7-dihydroxy-4 0 -methoxyflavone and 5,6,7,8-tetrahydroxy-4 0 -methoxyflavone never previously reported in plants, were isolated. The known 3,5,6,7,8-pentahydroxy-4 0 -methoxyflavone, 2 0 ,3 0 ,4 0 ,5 0 ,6 0 -pentahydroxy-chalcone, 6-hydroxykaempferol, 4 0 ,5,6,7,8-pentahydroxyflavone and 3,4 0 -dimethoxykaempferol were also obtained. Analysis of extracts obtained from roots and shoots revealed that the compositions of the flavonoid mixtures varied with growing conditions. Quercetin, acacetin and rhamnetin accumulated in roots of inoculated plants, whereas they were not detected in non-inoculated plants. 2004 Published by Elsevier Ltd.
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flavonoids from shoots and roots of trifolium repens white clover grown in presence or absence of the arbuscular mycorrhizal fungus Glomus Intraradices
Phytochemistry, 2004Co-Authors: Maria A Ponce, J A Ocampo, Jose M Scervino, Rosa Errabalsells, Alicia Margarita GodeasAbstract:White clover (Trifolium repens) plants were grown in the presence or absence of the arbuscular mycorrhizal fungus Glomus Intraradices. Flavones, 4',5,6,7,8-pentahydroxy-3-methoxyflavone and 5,6,7,8-tetrahydroxy-3-methoxyflavone, as well as two flavones 3,7-dihydroxy-4'-methoxyflavone and 5,6,7,8-tetrahydroxy-4'-methoxyflavone never previously reported in plants, were isolated. The known 3,5,6,7,8-pentahydroxy-4'-methoxyflavone, 2',3',4',5',6'-pentahydroxy-chalcone, 6-hydroxykaempferol, 4',5,6,7,8-pentahydroxyflavone and 3,4'-dimethoxykaempferol were also obtained. Analysis of extracts obtained from roots and shoots revealed that the compositions of the flavonoid mixtures varied with growing conditions. Quercetin, acacetin and rhamnetin accumulated in roots of inoculated plants, whereas they were not detected in non-inoculated plants.
Paola Lax - One of the best experts on this subject based on the ideXlab platform.
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Effect of the arbuscular mycorrhizal fungus Glomus Intraradices on the false root-knot nematode Nacobbus aberrans in tomato plants
Biology and Fertility of Soils, 2011Co-Authors: Paola Lax, Alejandra G. Becerra, Florencia Soteras, Marta Cabello, Marcelo E. DoucetAbstract:Information about the interaction between arbuscular mycorrhizal fungi (AMF) and the false root-knot nematode Nacobbus aberrans (Thorne, 1935) Thorne & Allen, 1944 is scarce. The effect of Glomus Intraradices Schenk & Smith on tomato ( Lycopersicon esculentum L.) cv. Platense inoculated with nematode juveniles from Lisandro Olmos (Argentina) was studied under greenhouse conditions. Six treatments with five replications were performed. After 80 days, nematode reproduction and percentage of AMF colonization in roots were estimated. Some plant growth parameters were also measured. In general, plants with AMF and AMF plus nematodes grew as well as the control without AMF and without nematodes. Furthermore, G. Intraradices was beneficial in reducing nematode-induced damage in roots (lower number of galls) as well as in having a suppressive effect on parasite reproduction. This is the first study on the use of G. Intraradices as a possible strategy in the control of N. aberrans in tomato.