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Antonio De Harobailon - One of the best experts on this subject based on the ideXlab platform.

  • quantification of glucosinolates in leaves of leaf rape brassica napus ssp pabularia by near infrared spectroscopy
    Phytochemistry, 2005
    Co-Authors: Rafael Font, Mercedes Del Riocelestino, Elena Cartea, Antonio De Harobailon
    Abstract:

    Abstract The potential of near-infrared spectroscopy (NIRS) for screening the total glucosinolate (t-GSL) content, and also, the aliphatic glucosinolates Gluconapin (GNA), glucobrassicanapin (GBN), progoitrin (PRO), glucoalyssin (GAL), and the indole glucosinolate glucobrassicin (GBS) in the leaf rape ( Brassica napus L. ssp. pabularia DC), was assessed. This crop is grown for edible leaves for both fodder and human consumption. In Galicia (northwestern Spain) it is highly appreciated for human nutrition and have the common name of “nabicol”. A collection of 36 local populations of nabicol was analysed by NIRS for glucosinolate composition. The reference values for glucosinolates, as they were obtained by high performance liquid chromatography on the leaf samples, were regressed against different spectral transformations by modified partial least-squares (MPLS) regression. The coefficients of determination in cross-validation ( r 2 ) shown by the equations for t-GSL, GNA, GBN, PRO, GAL and GBS were, respectively, 0.88, 0.73, 0.81, 0.78, 0.37 and 0.41. The standard deviation to standard error of cross-validation ratio, were for these constituents, as follows: t-GSL, 2.96; GNA, 1.94; GBN, 2.31; PRO, 2.11; GAL, 1.27, and GBS, 1.29. These results show that the equations developed for total glucosinolates, as well as those for Gluconapin, glucobrassicanapin and progoitrin, can be used for screening these compounds in the leaves of this species. In addition, the glucoalyssin and glucobrassicin equations obtained, can be used to identify those samples with low and high contents. From the study of the MPLS loadings of the first three terms of the different equations, it can be concluded that some major cell components as protein and cellulose, highly participated in modelling the equations for glucosinolates.

  • use of near infrared spectroscopy for screening the individual and total glucosinolate contents in indian mustard seed brassica juncea l czern coss
    Journal of Agricultural and Food Chemistry, 2004
    Co-Authors: Rafael Font, Jose M Fernandezmartinez, Antonio De Harobailon
    Abstract:

    The potential of near-infrared spectroscopy (NIRS) for screening the sinigrin, Gluconapin, 4-hydroxyglucobrassicin, and total glucosinolate contents of Indian mustard (Brassica juncea L. Czern. & Coss.) seed was assessed. Intact seed samples of this species were analyzed by NIRS and their reference values regressed against different spectral transformations by modified partial least-squares (MPLS) regression. The coefficients of determination (r 2) for sinigrin, Gluconapin, 4-hydroxyglucobrassicin, and total glucosinolate contents were, respectively, 0.86, 0.95, 0.33, and 0.82. The standard deviation to standard error of prediction (SEP) ratio, and SEP to standard error of laboratory ratio were for these constituents as follows:  sinigrin, 2.59 and 2.70; Gluconapin, 4.16 and 2.08; 4-hydroxyglucobrassicin, 1.18 and 1.40; and total glucosinolates, 2.18 and 1.60. By comparison of commercial sinigrin spectrum with the first MPLS loadings of the sinigrin equation, it can be concluded that the molecule of sinig...

Ruud Verkerk - One of the best experts on this subject based on the ideXlab platform.

  • Overexpression of the MYB29 transcription factor affects aliphatic glucosinolate synthesis in Brassica oleracea
    Plant Molecular Biology, 2019
    Co-Authors: Diana L. Zuluaga, Ruud Verkerk, Neil S. Graham, Annett Klinder, A. E. Elaine Ommen Kloeke, Angelo R. Marcotrigiano, Carol Wagstaff, Gabriella Sonnante, Mark G. M. Aarts
    Abstract:

    Key message Overexpression of BoMYB29 gene up-regulates the aliphatic glucosinolate pathway in Brassica oleracea plants increasing the production of the anti-cancer metabolite glucoraphanin, and the toxic and pungent sinigrin. Abstract Isothiocyanates, the bio-active hydrolysis products of glucosinolates, naturally produced by several Brassicaceae species, play an important role in human health and agriculture. This study aims at correlating the content of aliphatic glucosinolates to the expression of genes involved in their synthesis in Brassica oleracea , and perform functional analysis of BoMYB29 gene. To this purpose, three genotypes were used: a sprouting broccoli, a cabbage, and a wild genotype (Winspit), a high glucosinolate containing accession. Winspit showed the highest transcript level of BoMYB28 , BoMYB29 and BoAOP2 genes, and BoAOP2 expression was positively correlated with that of the two MYB genes. Further analyses of the aliphatic glucosinolates also showed a positive correlation between the expression of BoAOP2 and the production of sinigrin and Gluconapin in Winspit. The Winspit BoMYB29 CDS was cloned and overexpressed in Winspit and in the DH AG1012 line. Overexpressing Winspit plants produced higher quantities of alkenyl glucosinolates, such as sinigrin. Conversely, the DH AG1012 transformants showed a higher production of methylsulphinylalkyl glucosinolates, including glucoraphanin, and, despite an up-regulation of the aliphatic glucosinolate genes, no increase in alkenyl glucosinolates. The latter may be explained by the absence of a functional AOP2 gene in DH AG1012. Nevertheless, an extract of DH AG1012 lines overexpressing BoMYB29 provided a chemoprotective effect on human colon cells. This work exemplifies how the genetic diversity of B. oleracea may be used by breeders to select for higher expression of transcription factors for glucosinolate biosynthesis to improve its natural, health-promoting properties.

  • Stir-Frying of Chinese Cabbage and Pakchoi Retains Health-Promoting Glucosinolates
    Plant Foods for Human Nutrition, 2017
    Co-Authors: Probo Y. Nugrahedi, Jenneke K. Heising, Teresa Oliviero, Matthijs Dekker, Ruud Verkerk
    Abstract:

    Stir-frying is a cooking method, originating from Asia, in which food is fried in small amount of very hot oil. Nowadays in many other parts of the world stir-frying is a very popular method to prepare vegetables, because it is fast and fried vegetables are tasty. However, the retention of phytochemicals like the health-beneficial glucosinolates in Brassica vegetables is less explored for stir-frying in comparison to other cooking methods. This study investigates the retention of glucosinolates in Chinese cabbage (Brassica rapa ssp. pekinensis) and pakchoi (Brassica rapa ssp. chinensis) as affected by stir-frying at various cooking durations and temperatures. Stir-frying experiments were performed at set pan temperatures ranging from 160 to 250 °C for a duration of 1 to 8 min. Results showed that aliphatic glucobrassicanapin is the most abundant glucosinolate identified in fresh Chinese cabbage and pakchoi, contributing for 48 and 63% of the total glucosinolate content, respectively, followed by glucoiberin and Gluconapin. Stir-frying retains the glucosinolates even at the highest temperature applied. Such retention is explained by the quick inactivation of the glucosinolate-hydrolytic enzyme myrosinase during the first minutes of frying, and by the thermal stability of the glucosinolates at those temperature/time conditions. Moreover, due to the absence of a separate water phase, leaching losses did not occur, in contrast to what is observed when boiling Brassica vegetables. These results show that stir-frying may be a suitable health-beneficial cooking option that prevents the loss of glucosinolates.

  • Genetic variation in glucosinolate content within Brassica rapa vegetables
    Acta Horticulturae, 2012
    Co-Authors: Lou Ping, Matthijs Dekker, Guusje Bonnema, Ruud Verkerk
    Abstract:

    Glucosinolates (GSs) were analyzed in 56 accessions of Brassica rapa grown in the greenhouse. Eight different glucosinolates were identified in the Brassica rapa group. They are the aliphatic glucosinolates progoitrin (PRO), Gluconapin (NAP), glucobrassicanapin (GBN), the indolyl glucosinolates 4-hydroxyglucobrassicin (4OH), glucobrassicin (GBC), 4-methoxyglucobrassicin (4ME), neoglucobrassicin (NEO) and the aromatic glucosinolate gluconasturtiin (NAS). Gluconapin, glucobrassicanapin, progoitrin and gluconasturtiin are the most abundant GSs in the Brassica rapa, but there is considerable variation in content among accessions. The total glucosinolate contents in Brassica rapa group varied substantially between the different accessions. The highest amount of GSs (361 µmol/100 g FW) was observed in leaves of vegetable turnip, followed by rapid cycling and yellow sarson, with the amount of 200 and 178 µmol/100 g FW respectively. Whereas the lowest GSs content was found in turnip greens (20.8 µmol/100 g FW) and Wutacai (22.6 µmol/100 g FW). The total aliphatic GSs proportion varied from 50 to 90% of the total GS, while generally the content of indolyl glucosinoloates, especially 4OH glucobrassicin and neoglucobrassicin is low. Gluconasturtiin was found in relatively high concentrations in komastsuna (14.6 µmol/100 g FW), yellow sarson (7.1 µmol/ 100 g FW) and constitutes as much as 24% of the total amount of glucosinolates. Relatively high amounts of Gluconapin (281 mmol/100 g FW) and glucobrassicanapin (60.0 mmol/100 g FW) were observed in the leaves of vegetable turnip. Compared with the Brassica oleracea group, Brassica rapa lacks glucoraphanin and sinigrin but contains Gluconapin and glucobrassicanapin. Variations in glucosinolate content among genotypes suggest differences in their health-promoting properties and the opportunity for enhancement of their levels through breeding or genetic modification.

Rafael Font - One of the best experts on this subject based on the ideXlab platform.

  • quantification of glucosinolates in leaves of leaf rape brassica napus ssp pabularia by near infrared spectroscopy
    Phytochemistry, 2005
    Co-Authors: Rafael Font, Mercedes Del Riocelestino, Elena Cartea, Antonio De Harobailon
    Abstract:

    Abstract The potential of near-infrared spectroscopy (NIRS) for screening the total glucosinolate (t-GSL) content, and also, the aliphatic glucosinolates Gluconapin (GNA), glucobrassicanapin (GBN), progoitrin (PRO), glucoalyssin (GAL), and the indole glucosinolate glucobrassicin (GBS) in the leaf rape ( Brassica napus L. ssp. pabularia DC), was assessed. This crop is grown for edible leaves for both fodder and human consumption. In Galicia (northwestern Spain) it is highly appreciated for human nutrition and have the common name of “nabicol”. A collection of 36 local populations of nabicol was analysed by NIRS for glucosinolate composition. The reference values for glucosinolates, as they were obtained by high performance liquid chromatography on the leaf samples, were regressed against different spectral transformations by modified partial least-squares (MPLS) regression. The coefficients of determination in cross-validation ( r 2 ) shown by the equations for t-GSL, GNA, GBN, PRO, GAL and GBS were, respectively, 0.88, 0.73, 0.81, 0.78, 0.37 and 0.41. The standard deviation to standard error of cross-validation ratio, were for these constituents, as follows: t-GSL, 2.96; GNA, 1.94; GBN, 2.31; PRO, 2.11; GAL, 1.27, and GBS, 1.29. These results show that the equations developed for total glucosinolates, as well as those for Gluconapin, glucobrassicanapin and progoitrin, can be used for screening these compounds in the leaves of this species. In addition, the glucoalyssin and glucobrassicin equations obtained, can be used to identify those samples with low and high contents. From the study of the MPLS loadings of the first three terms of the different equations, it can be concluded that some major cell components as protein and cellulose, highly participated in modelling the equations for glucosinolates.

  • use of near infrared spectroscopy for screening the individual and total glucosinolate contents in indian mustard seed brassica juncea l czern coss
    Journal of Agricultural and Food Chemistry, 2004
    Co-Authors: Rafael Font, Jose M Fernandezmartinez, Antonio De Harobailon
    Abstract:

    The potential of near-infrared spectroscopy (NIRS) for screening the sinigrin, Gluconapin, 4-hydroxyglucobrassicin, and total glucosinolate contents of Indian mustard (Brassica juncea L. Czern. & Coss.) seed was assessed. Intact seed samples of this species were analyzed by NIRS and their reference values regressed against different spectral transformations by modified partial least-squares (MPLS) regression. The coefficients of determination (r 2) for sinigrin, Gluconapin, 4-hydroxyglucobrassicin, and total glucosinolate contents were, respectively, 0.86, 0.95, 0.33, and 0.82. The standard deviation to standard error of prediction (SEP) ratio, and SEP to standard error of laboratory ratio were for these constituents as follows:  sinigrin, 2.59 and 2.70; Gluconapin, 4.16 and 2.08; 4-hydroxyglucobrassicin, 1.18 and 1.40; and total glucosinolates, 2.18 and 1.60. By comparison of commercial sinigrin spectrum with the first MPLS loadings of the sinigrin equation, it can be concluded that the molecule of sinig...

R T Zijlstra - One of the best experts on this subject based on the ideXlab platform.

  • diet nutrient digestibility and growth performance of weaned pigs fed solvent extracted brassica juncea canola meal
    Animal Feed Science and Technology, 2013
    Co-Authors: J L Landero, E Beltranena, R T Zijlstra
    Abstract:

    The effects of substituting soybean meal (SBM) with increasing levels of solvent-extracted canola meal (CM) derived from modern Brassica juncea were evaluated on diet nutrient digestibility and growth performance of young pigs. In total, 240 weaned pigs with an initial body weight of 7.7 kg, starting 1 week after weaning at 19 days of age were involved. Pigs were fed Phase 1 test diets for 2 weeks (days 0–14) and sequentially Phase 2 test diets for 3 weeks (days 15–35). Five pelleted wheat-based diets containing 0, 60, 120, 180 or 240 g juncea CM/kg were formulated to contain 10.0 and 9.7 MJ net energy (NE)/kg and 1.2 and 1.1 g standardised ileal digestible (SID) lysine/MJ NE, for the Phase 1 and 2 diets, respectively. Juncea CM was added at the expense of SBM and the diets were balanced for NE by increasing canola oil from 56 to 80 and 26 to 50 g/kg for Phase 1 and 2 diets, respectively, and for amino acids by increasing crystalline amino acids. Increasing inclusion of juncea CM linearly reduced (P<0.001) the apparent total tract digestibility of gross energy, dry matter, and crude protein for both feeding phases. Increasing inclusion of juncea CM also decreased diet digestible energy values in Phase 1 (P<0.001) and Phase 2 (P<0.05). For the entire trial (days 0–35), increasing inclusion of juncea CM linearly reduced (P<0.01) body weight gain, feed intake and feed efficiency (gain:feed). At the end of the experiment, pigs fed 60, 120, 180 and 240 g juncea CM/kg were 0.9, 1.1, 1.3 and 1.9 kg lighter than pigs not fed juncea CM. In conclusion, substitution of SBM with increasing inclusion of juncea CM in nursery diets formulated to equal NE and SID amino acid content linearly reduced diet nutrient digestibility and growth performance of weaned pigs in a dose–response manner. These reductions were likely due to a sensitivity of young pigs to the glucosinolate Gluconapin that is the most abundant in juncea CM or high dietary crude fat content due to oil addition.

  • growth performance and preference studies to evaluate solvent extracted brassica napus or brassica juncea canola meal fed to weaned pigs1
    Journal of Animal Science, 2012
    Co-Authors: J L Landero, E Beltranena, R T Zijlstra
    Abstract:

    : Inclusion of conventional dark-seeded (Brassica napus) and novel yellow-seeded (Brassica juncea) canola meal (CM) can potentially replace soybean (Glycine max) meal (SBM) in pig diets. Our objective was to examine the preference of weaned pigs fed diets containing SBM or B. napus or B. juncea CM and to compare it against previously reported growth performance data (Exp. 1 and 2). In Exp. 1 and 2, growth performance was evaluated using 220 and 240 weaned pigs, respectively, by replacing dietary SBM with up to 20% B. napus (Exp. 1) or 24% B. juncea CM (Exp. 2). Feeding up to 20% B. napus CM to pigs did not affect growth performance, but increasing inclusion of B. juncea CM linearly reduced (P < 0.001) ADFI, ADG, and G:F most likely due to the higher content of glucosinolates, particularly Gluconapin in B. juncea CM as confirmed by principle component analysis. In Exp. 3 and 4, SBM and B. napus and B. juncea CM fed at 20% dietary inclusion were evaluated in 2 preference studies using 216 and 144 pigs of 35 d of age, respectively. Pens equipped with 2 feeders housed 8 or 4 pigs per pen, in Exp. 3 and 4, respectively. Diets formulated to equal NE and standardized ileal digestible AA were offered in a paired choice as mash (Exp. 3) or pellets (Exp. 4) for 3 consecutive 7-d periods (3 d nontest and 4 d preference test). The 3 treatments offered were (i) SBM vs. B. napus CM, (ii) SBM vs. B. juncea CM, and (iii) B. napus vs. B. juncea CM. Pigs preferred SBM (P < 0.001) over B. napus and B. juncea CM diets, and pigs preferred B. napus (P < 0.001) over B. juncea CM diet. High content of the glucosinolate Gluconapin likely reduced feed preference in B. juncea more than in B. napus CM. In conclusion, the contrast between preference and performance studies feeding CM to pigs indicates that preference studies should be interpreted cautiously until validated by growth performance data.

Sunju Kim - One of the best experts on this subject based on the ideXlab platform.

  • Effect of different proportion of sulphur treatments on the contents of glucosinolate in kale (Brassica oleracea var. acephala) commonly consumed in Republic of Korea
    Elsevier, 2018
    Co-Authors: Ye-jin Park, Mariadhas Valan Arasu, Naif Abdullah Al-dhabi, Hye-min Lee, Minjung Shin, Doug Young Chung, Sunju Kim
    Abstract:

    Kale (Brassica oleracea L. Acephala Group) is the rich source of medicinal value sulphur compounds, glucosinolates (GLSs). The aim of this study was to investigate the effect of different proportion of sulphur (S) supplementation levels on the accumulation of GLSs in the leaves of the kale cultivar ('TBC'). High performance liquid chromatography (HPLC) separation method guided to identify and quantify six GSLs including three aliphatic (progoitrin, sinigrin and Gluconapin) and three indolyl (glucobrassicin, 4-methoxyglucobrassicin and neoglucobrasscin) respectively. Analysis of these distinct levels of S supplementation revealed that the accumulation of individual and total GLSs was directly proportional to the S concentration. The maximum levels of total GLSs (26.8 µmol/g DW) and glucobrassicin (9.98 µmol/g DW) were found in lower and upper parts of the leaves supplemented with 1 mM and 2 mM S, respectively. Interestingly, aliphatic GSLs were noted predominant in all the parts (50.1, 59.3 and 56% of total GSLs). Among the aliphatic and indolyl GSLs, sinigrin and glucobrassicin account 35.3 and 30.88% of the total GSLs. From this study, it is concluded that supply of S enhance the GSLs accumulation in kale

  • Variation in G lucosinolate C ontents of C ruciferous P lants
    ACG Publications, 2017
    Co-Authors: Won Park, Sunju Kim, Kwang-soo Kim, Young-seok Jang, Kyungbo Lee, Sung-ju Ahn, Suk Whan Hong, Yong-hwa Lee
    Abstract:

    Glucosinolates are secondary metabolites of almost all plants of the order Brassicales, and have been known to control nematode populations. In this study, 14 glucosinolates were identified, quantified, and compared in several varieties and cultivars of cruciferous plants including Brassica campestris ssp. pekinensis (Chinese cabbage), Brassica juncea var. crispifolia L. H. Bailey (mustard), Brassica juncea L. Czern. var. juncea (leaf mustard), Brassica oleracea L. var. acephala (kale), Raphanus sativus L. (radish), and Brassica campestris L. ssp. oleifera (winter turnip rape). The most abundant glucosinolate in mustard, leaf mustard, kale, and radish was sinigrin. In leaf mustard, the sinigrin content ranged from 193.05 μmol/g to 215.52 μmol/g, and in mustard, the sinigrin contents of blue mustard and red mustard were 219.08 μmol/g and 215.73 μmol/g, respectively. Kale and radish contained 137.79 μmol/g and 120.25 μmol/g, respectively, of sinigrin. Gluconapin was the most abundant glucosinolate in winter turnip rape, at 121.17 μmol/g. Chinese cabbage contained mostly glucocochlearin (79.88 μmol/g). These results will be useful in the development of environmentally friendly plant-based pesticides by allowing for proper control of glucosinolates based on those present in the chosen plant species

  • variation of glucoraphanin and glucobrassicin anticancer components in brassica during processing
    Food Science and Technology International, 2013
    Co-Authors: Me-hea Park, Mariadhas Valan Arasu, Naif Abdullah Aldhabi, No-young Park, Yun-jong Choi, Sang-won Lee, Jung Bong Kim, Sunju Kim
    Abstract:

    Effects of cold storage and three common cooking practices, blanching, sauteing, and microwave cooking at different time intervals, on the content of glucosinolate (GSL) anticancer components in six Brassica vegetables were investigated. Eleven GSLs including progoitrin, glucoraphanin, sinigrin, glucoalyssin, Gluconapin, glucobrassicanapin, glucoerucin, glucobrassicin, 4-methoxyglucobrassicin, gluconasturtiin, and neoglucobrassicin were quantified using LC-MS and HPLC. Storage at 4 oC indicated no significant loss of GSLs in broccoli, kohlrabi, and cabbage, and approximately 90-100% of the total concentration of aliphatic and indolyl GSLs were detected. Interestingly, glucoraphanin and glucobrassicin, known as a cancer prevention agents, increased approximately above 50% in broccoli, kohlrabi, and cabbage, while the amount of glucobrassicin decreased by 5% in cauliflower for 5 days at 4 oC. Blanching of broccoli at 120 sec significantly (36%) decreased total GSLs; however, sauteing and microwaving decreased by13-26%. Individual GSLs have different response at blanching. These findings suggest that different processing methods for each vegetable would be preferred to preserve the nutritional qualities.

  • Variation of glucoraphanin and glucobrassicin: anticancer components in Brassica during processing
    Sociedade Brasileira de Ciência e Tecnologia de Alimentos, 2013
    Co-Authors: Me-hea Park, Mariadhas Valan Arasu, No-young Park, Yun-jong Choi, Sang-won Lee, Naif Abdullah Al-dhabi, Jung Bong Kim, Sunju Kim
    Abstract:

    Effects of cold storage and three common cooking practices, blanching, sauteing, and microwave cooking at different time intervals, on the content of glucosinolate (GSL) anticancer components in six Brassica vegetables were investigated. Eleven GSLs including progoitrin, glucoraphanin, sinigrin, glucoalyssin, Gluconapin, glucobrassicanapin, glucoerucin, glucobrassicin, 4-methoxyglucobrassicin, gluconasturtiin, and neoglucobrassicin were quantified using LC-MS and HPLC. Storage at 4 ºC indicated no significant loss of GSLs in broccoli, kohlrabi, and cabbage, and approximately 90-100% of the total concentration of aliphatic and indolyl GSLs were detected. Interestingly, glucoraphanin and glucobrassicin, known as a cancer prevention agents, increased approximately above 50% in broccoli, kohlrabi, and cabbage, while the amount of glucobrassicin decreased by 5% in cauliflower for 5 days at 4 ºC. Blanching of broccoli at 120 sec significantly (36%) decreased total GSLs; however, sautéing and microwaving decreased by13-26%. Individual GSLs have different response at blanching. These findings suggest that different processing methods for each vegetable would be preferred to preserve the nutritional qualities

  • MYB Transcription Factors Regulate Glucosinolate Biosynthesis in Different Organs of Chinese Cabbage (Brassica rapa ssp. pekinensis)
    MDPI AG, 2013
    Co-Authors: Hyeran Kim, Sunju Kim, Sang Un Park, Haeng Hoon Kim, Jeongyeo Lee, Yeon Bok Kim
    Abstract:

    In this study, we investigated the expression of seven MYB transcription factors (a total of 17 genes that included Dof1.1, IQD1-1, MYB28, MYB29, MYB34, MYB51, and MYB122 and their isoforms) involved in aliphatic and indolic glucosinolate (GSL) biosynthesis and analyzed the aliphatic and indolic GSL content in different organs of Chinese cabbage (Brassica rapassp. Pekinensis). MYB28 and MYB29 expression in the stem was dramatically different when compared with the levels in the other organs. MYB34, MYB122, MYB51, Dof1.1, and IQD1-1 showed very low transcript levels among different organs. HPLC analysis showed that the glucosinolates (GSLs) consisted of five aliphatic GSLs (progoitrin, sinigrin, glucoalyssin, Gluconapin, and glucobrassicanapin) and four indolic GSLs (4-hydroxyglucobrassicin, glucobrassicin, 4-methoxygluco-brassicin, and neoglucobrassicin). Aliphatic GSLs exhibited 63.3% of the total GSLs content, followed by aromatic GSL (19.0%), indolic GSLs (10%), and unknown GSLs (7.7%) in different organs of Chinese cabbage. The total GSL content of different parts (ranked in descending order) was as follows: seed > flower > young leaves > stem > root > old leaves. The relationship between GSLs accumulation and expression of GSLs biosynthesis MYB TFs genes in different organs may be helpful to understand the mechanism of MYB TFs regulating GSL biosynthesis in Chinese cabbage