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Rotimi E. Aluko - One of the best experts on this subject based on the ideXlab platform.

  • C: Food Chemistry Structural and Functional Properties of Hemp Seed Protein Products
    2020
    Co-Authors: Sunday A Malomo, Rong He, Rotimi E. Aluko
    Abstract:

    The effects of pH and protein concentration on some structural and functional properties of Hemp Seed protein isolate (HPI, 84.15% protein content) and defatted Hemp Seed protein meal (HPM, 44.32% protein content) were determined. The HPI had minimum protein solubility (PS) at pH 4.0, which increased as pH was decreased or increased. In contrast, the HPM had minimum PS at pH 3.0, which increased at higher pH values. Gel electrophoresis showed that some of the high molecular weight proteins (>45 kDa) present in HPM were not well extracted by the alkali and were absent or present in low ratio in the HPI polypeptide profile. The amino acid composition showed that the isolation process increased the Arg/Lys ratio of HPI (5.52%) when compared to HPM (3.35%). Intrinsic fluorescence and circular dichroism data indicate that the HPI proteins had a well-defined structure at pH 3.0, which was lost as pH value increased. The differences in structural conformation of HPI at different pH values were reflected as better foaming capacity at pH 3.0 when compared to pH 5.0, 7.0, and 9.0. At 10 and 25 mg/mL protein concentrations, emulsions formed by the HPM had smaller oil droplet sizes (higher quality), when compared to the HPI-formed emulsions. In contrast at 50 mg/mL protein concentration, the HPI-formed emulsions had smaller oil droplet sizes (except at pH 3.0). We conclude that the functional properties of Hemp Seed protein products are dependent on structural conformations as well as protein concentration and pH. Practical Application: Hemp Seed is a popular oil Seed crop grown in Canada and globally for its oil and protein products. Hemp Seed proteins (HSPs), which account for 25% of its composition, have been widely studied in detail, but their structural and functional properties at varying processing conditions are not well understood. This work provides information on the structural conformation and functional performance in model systems of HSP under varying pH conditions that cover the food processing range. The presented data could enhance manipulation of environmental pH conditions for the use of HSP as novel food ingredients.

  • a double blind randomized crossover trial protocol of whole Hemp Seed protein and Hemp Seed protein hydrolysate consumption for hypertension
    Trials, 2020
    Co-Authors: Maryam Samsamikor, Rebecca C. Mollard, Dylan S Mackay, Rotimi E. Aluko
    Abstract:

    BACKGROUND: Primary hypertension accounts for almost 95% of all cases of high blood pressure and is a major modifiable risk factor for cardiovascular diseases. Lifestyle interventions have been shown to prevent hypertension. One of the prominent potential therapeutic lifestyle strategies to prevent or manage hypertension is increasing dietary protein as a macronutrient or as bioactive peptides. An emerging plant-based protein source that may have anti-hypertensive properties is Hemp Seed. METHODS/DESIGN: A randomized, double-blind, crossover clinical trial will be conducted on 35 hypertensive participants aged 18-75 years, with a BMI between 18.5 and 40 kg/m2, systolic blood pressure (SBP) between 130 and 160 mmHg and diastolic blood pressure (DBP) ≤ 110 mmHg. The trial will be conducted for a period of 22 weeks and will consist of three treatment periods of 6 weeks, separated by 2-week washout periods. The treatments will be consumed twice a day and consist of 25 g casein, Hemp Seed protein (HSP), or HSP plus HSP hydrolysate (HSP+). The primary outcome of this trial is 24-h SBP, measured on the first day of first phase and the last day of each phase. Office-measured blood pressure, pulse-wave velocity and augmentation index and anthropometrics will be determined at the first and last days of each period. Also, body composition will be assessed by dual x-ray absorptiometry (DXA) scan on the first day of the first phase and within the last 2 days of each treatment period. Blood samples will be collected on the first and last 2 days of each treatment phase whereas urine samples will be collected on the first day of the first phase plus the last day of each phase to be analyzed for specific biomarkers. DISCUSSION: This trial protocol is designed to evaluate the hypotensive potential of consuming whole HSP, and HSP+, in comparison to casein protein. This study will be the first trial investigating the potential anti-hypertensive benefit of dietary Hemp protein plus bioactive peptide consumption in humans. TRIAL REGISTRATION: National Clinical Trial (NCT), ID: NCT03508895. Registered on 28 June 2018. Retrospectively registered on the publicly accessible Registry Databank at ClinicalTrials.gov (http://ClinicalTrials.gov).

  • A double-blind, randomized, crossover trial protocol of whole Hemp Seed protein and Hemp Seed protein hydrolysate consumption for hypertension
    Trials, 2020
    Co-Authors: Maryam Samsamikor, Dylan Mackay, Rebecca C. Mollard, Rotimi E. Aluko
    Abstract:

    Background Primary hypertension accounts for almost 95% of all cases of high blood pressure and is a major modifiable risk factor for cardiovascular diseases. Lifestyle interventions have been shown to prevent hypertension. One of the prominent potential therapeutic lifestyle strategies to prevent or manage hypertension is increasing dietary protein as a macronutrient or as bioactive peptides. An emerging plant-based protein source that may have anti-hypertensive properties is Hemp Seed. Methods/design A randomized, double-blind, crossover clinical trial will be conducted on 35 hypertensive participants aged 18–75 years, with a BMI between 18.5 and 40 kg/m^2, systolic blood pressure (SBP) between 130 and 160 mmHg and diastolic blood pressure (DBP) ≤ 110 mmHg. The trial will be conducted for a period of 22 weeks and will consist of three treatment periods of 6 weeks, separated by 2-week washout periods. The treatments will be consumed twice a day and consist of 25 g casein, Hemp Seed protein (HSP), or HSP plus HSP hydrolysate (HSP+). The primary outcome of this trial is 24-h SBP, measured on the first day of first phase and the last day of each phase. Office-measured blood pressure, pulse-wave velocity and augmentation index and anthropometrics will be determined at the first and last days of each period. Also, body composition will be assessed by dual x-ray absorptiometry (DXA) scan on the first day of the first phase and within the last 2 days of each treatment period. Blood samples will be collected on the first and last 2 days of each treatment phase whereas urine samples will be collected on the first day of the first phase plus the last day of each phase to be analyzed for specific biomarkers. Discussion This trial protocol is designed to evaluate the hypotensive potential of consuming whole HSP, and HSP+, in comparison to casein protein. This study will be the first trial investigating the potential anti-hypertensive benefit of dietary Hemp protein plus bioactive peptide consumption in humans. Trial registration National Clinical Trial (NCT), ID: NCT03508895 . Registered on 28 June 2018. Retrospectively registered on the publicly accessible Registry Databank at ClinicalTrials.gov ( http://ClinicalTrials.gov ).

  • Kinetics of acetylcholinesterase inhibition by Hemp Seed protein-derived peptides.
    Journal of Food Biochemistry, 2019
    Co-Authors: Sunday A Malomo, Rotimi E. Aluko
    Abstract:

    The aim of this work was to enhance the acetylcholinesterase (AChE)-inhibitory activity of a pepsin-produced Hemp Seed protein hydrolysates (HPH) through reverse-phase HPLC separation followed by identification of peptide sequences present in the most active fraction. The HPH was separated into eight fractions (F1-F8) with F7 exhibiting significantly (p 

  • in vitro acetylcholinesterase inhibitory properties of enzymatic Hemp Seed protein hydrolysates
    Journal of the American Oil Chemists' Society, 2016
    Co-Authors: Sunday A Malomo, Rotimi E. Aluko
    Abstract:

    The aim of this work was to characterize the structural and functional properties of Hemp Seed protein-derived acetylcholinesterase (AChE)-inhibitory enzymatic hydrolysates. Hemp Seed protein isolate hydrolysis was performed using six different proteases (pepsin, papain, thermoase, flavourzyme, alcalase and pepsin + pancreatin) at different concentrations (1–4 %). The degree of hydrolysis was directly related to the amount of protease used but had no relationship with AChE-inhibitory activity. Amino acid composition results showed that the Hemp Seed protein hydrolysates (HPHs) had high levels of negatively charged amino acids (39.62–40.18 %) as well as arginine. The 1 % pepsin HPH was the most active AChE inhibitor with ~6 µg/mL IC50 value when compared to 8–11.6 µg/mL for the other HPHs. Mass spectrometry analysis showed that most of the peptides in all the hydrolysates were less than 1000 Da in size. However, the pepsin HPHs contained larger-sized peptides (244–1009 Da) than the papain HPHs (246–758 Da), which in turn was larger than the alcalase HPH (246–607 Da). The higher AChE-inhibitory effects of the pepsin HPHs may be due to increased synergistic effects from a wider peptide size range when compared to the papain and alcalase HPHs that had narrower ranges. The narrow peptide size range in the alcalase HPH confirms the higher efficiency of this protease in releasing small-sized peptides from food proteins.

Abraham T Girgih - One of the best experts on this subject based on the ideXlab platform.

  • structural and antihypertensive properties of enzymatic Hemp Seed protein hydrolysates
    Nutrients, 2015
    Co-Authors: Sunday A Malomo, Abraham T Girgih, John O Onuh, Rotimi E. Aluko
    Abstract:

    The aim of this work was to produce antihypertensive protein hydrolysates through different forms of enzymatic hydrolysis (2% pepsin, 4% pepsin, 1% alcalase, 2% alcalase, 2% papain, and 2% pepsin + pancreatin) of Hemp Seed proteins (HSP). The Hemp Seed protein hydrolysates (HPHs) were tested for in vitro inhibitions of renin and angiotensin-converting enzyme (ACE), two of the enzymes that regulate human blood pressure. The HPHs were then administered orally (200 mg/kg body weight) to spontaneously hypertensive rats and systolic blood pressure (SBP)-lowering effects measured over a 24 h period. Size exclusion chromatography mainly showed a 300–9560 Da peptide size range for the HPHs, while amino acid composition data had the 2% pepsin HPH with the highest cysteine content. Fluorescence spectroscopy revealed higher fluorescence intensities for the peptides when compared to the unhydrolyzed Hemp Seed protein. Overall, the 1% alcalase HPH was the most effective (p < 0.05) SBP-reducing agent (−32.5 ± 0.7 mmHg after 4 h), while the pepsin HPHs produced longer-lasting effects (−23.0 ± 1.4 mmHg after 24 h). We conclude that an optimized combination of the fast-acting HPH (1% alcalase) with the longer-lasting HPHs (2% and 4% pepsin) could provide daily effective SBP reductions.

  • a novel Hemp Seed meal protein hydrolysate reduces oxidative stress factors in spontaneously hypertensive rats
    Nutrients, 2014
    Co-Authors: Abraham T Girgih, Adeola Alashi, Sunday A Malomo, Rong He, Thomas Netticadan, Rotimi E. Aluko
    Abstract:

    This report shows the antioxidant effects of a Hemp Seed meal protein hydrolysate (HMH) in spontaneously hypertensive rats (SHR). Defatted Hemp Seed meal was hydrolyzed consecutively with pepsin and pancreatin to yield HMH, which was incorporated into rat feed as a source of antioxidant peptides. Young (8-week old) SHRs were divided into three groups (8 rats/group) and fed diets that contained 0.0%, 0.5% or 1.0% (w/w) HMH for eight weeks; half of the rats were sacrificed for blood collection. After a 4-week washout period, the remaining 20-week old SHRs were fed for an additional four weeks and sacrificed for blood collection. Plasma total antioxidant capacity (TAC) and superoxide dismutase (SOD), catalase (CAT) and total peroxides (TPx) levels were determined. Results showed that plasma TAC, CAT and SOD levels decreased in the older 20-week old SHRs when compared to the young SHRs. The presence of HMH in the diets led to significant (p < 0.05) increases in plasma SOD and CAT levels in both young and adult SHR groups; these increases were accompanied by decreases in TPx levels. The results suggest that HMH contained antioxidant peptides that reduced the rate of lipid peroxidation in SHRs with enhanced antioxidant enzyme levels and total antioxidant capacity.

  • Preventive and treatment effects of a Hemp Seed (Cannabis sativa L.) meal protein hydrolysate against high blood pressure in spontaneously hypertensive rats
    European Journal of Nutrition, 2014
    Co-Authors: Abraham T Girgih, Adeola Alashi, Rong He, S. Malomo, Rotimi E. Aluko
    Abstract:

    PurposeThis work determined the ability of Hemp Seed meal protein hydrolysate (HMH)-containing diets to attenuate elevated blood pressure (hypertension) development in spontaneously hypertensive rats (SHRs). Effects of diets on plasma levels of renin and angiotensin I-converting enzyme (ACE) in the SHRs were also determined.MethodsDefatted Hemp Seed protein meal was hydrolyzed using simulated gastrointestinal tract digestion with pepsin followed by pancreatin, and the resulting HMH used as a source of antihypertensive peptides. The HMH was substituted for casein at 0.5 and 1.0 % levels and fed to young growing rats for 8 weeks (preventive phase) or adult rats for 4 weeks (treatment phase).ResultsFeeding of young growing SHRs with HMH resulted in attenuation of the normal increases in systolic blood pressure (SBP) with an average value of ~120 mmHg when compared to the casein-only group of rats (control) with a maximum of 158 mm Hg (p 

  • preventive and treatment effects of a Hemp Seed cannabis sativa l meal protein hydrolysate against high blood pressure in spontaneously hypertensive rats
    European Journal of Nutrition, 2014
    Co-Authors: Abraham T Girgih, Adeola Alashi, Sunday A Malomo, Rong He, Rotimi E. Aluko
    Abstract:

    Purpose This work determined the ability of Hemp Seed meal protein hydrolysate (HMH)-containing diets to attenuate elevated blood pressure (hypertension) development in spontaneously hypertensive rats (SHRs). Effects of diets on plasma levels of renin and angiotensin I-converting enzyme (ACE) in the SHRs were also determined.

  • structural and functional characterization of Hemp Seed cannabis sativa l protein derived antioxidant and antihypertensive peptides
    Journal of Functional Foods, 2014
    Co-Authors: Abraham T Girgih, Sunday A Malomo, Marina Offengenden, Rong He, Jianping Wu, Rotimi E. Aluko
    Abstract:

    Abstract The aim of this study was to identify and test efficacy of antioxidant and antihypertensive peptides present in Hemp Seed protein hydrolysate (HPH), which was produced through simulated gastrointestinal tract digestion of Hemp Seed proteins. Consecutive fractionation of HPH by reverse-phase HPLC followed by tandem mass spectrometry analysis of active peaks led to identification of 23 short-chain (⩽5 amino acids) peptides. At 0.5 mg/mL, Trp-Val-Tyr-Tyr (WVYY) and Pro-Ser-Leu-Pro-Ala (PSLPA) were the most active antioxidant peptides with 67% and 58% DPPH scavenging and metal chelation activity of 94% and 96%, respectively. WVYY and PSLPA showed maximum systolic blood pressure (SBP) reduction in spontaneously hypertensive rats by 34 (2 h) and 40 mmHg (4 h), respectively, after oral administration of 30 mg/kg body weight dose. Trp-Tyr-Thr (WYT) at 2 h, Ser-Val-Tyr-Thr (SVYT) at 6 h, and Ile-Pro-Ala-Gly-Val (IPAGV) at 4 h, each at 30 mg/kg body weight dose had maximum SBP reductions of 13, 24, and 36 mmHg, respectively.

Sunday A Malomo - One of the best experts on this subject based on the ideXlab platform.

  • C: Food Chemistry Structural and Functional Properties of Hemp Seed Protein Products
    2020
    Co-Authors: Sunday A Malomo, Rong He, Rotimi E. Aluko
    Abstract:

    The effects of pH and protein concentration on some structural and functional properties of Hemp Seed protein isolate (HPI, 84.15% protein content) and defatted Hemp Seed protein meal (HPM, 44.32% protein content) were determined. The HPI had minimum protein solubility (PS) at pH 4.0, which increased as pH was decreased or increased. In contrast, the HPM had minimum PS at pH 3.0, which increased at higher pH values. Gel electrophoresis showed that some of the high molecular weight proteins (>45 kDa) present in HPM were not well extracted by the alkali and were absent or present in low ratio in the HPI polypeptide profile. The amino acid composition showed that the isolation process increased the Arg/Lys ratio of HPI (5.52%) when compared to HPM (3.35%). Intrinsic fluorescence and circular dichroism data indicate that the HPI proteins had a well-defined structure at pH 3.0, which was lost as pH value increased. The differences in structural conformation of HPI at different pH values were reflected as better foaming capacity at pH 3.0 when compared to pH 5.0, 7.0, and 9.0. At 10 and 25 mg/mL protein concentrations, emulsions formed by the HPM had smaller oil droplet sizes (higher quality), when compared to the HPI-formed emulsions. In contrast at 50 mg/mL protein concentration, the HPI-formed emulsions had smaller oil droplet sizes (except at pH 3.0). We conclude that the functional properties of Hemp Seed protein products are dependent on structural conformations as well as protein concentration and pH. Practical Application: Hemp Seed is a popular oil Seed crop grown in Canada and globally for its oil and protein products. Hemp Seed proteins (HSPs), which account for 25% of its composition, have been widely studied in detail, but their structural and functional properties at varying processing conditions are not well understood. This work provides information on the structural conformation and functional performance in model systems of HSP under varying pH conditions that cover the food processing range. The presented data could enhance manipulation of environmental pH conditions for the use of HSP as novel food ingredients.

  • Kinetics of acetylcholinesterase inhibition by Hemp Seed protein-derived peptides.
    Journal of Food Biochemistry, 2019
    Co-Authors: Sunday A Malomo, Rotimi E. Aluko
    Abstract:

    The aim of this work was to enhance the acetylcholinesterase (AChE)-inhibitory activity of a pepsin-produced Hemp Seed protein hydrolysates (HPH) through reverse-phase HPLC separation followed by identification of peptide sequences present in the most active fraction. The HPH was separated into eight fractions (F1-F8) with F7 exhibiting significantly (p 

  • in vitro acetylcholinesterase inhibitory properties of enzymatic Hemp Seed protein hydrolysates
    Journal of the American Oil Chemists' Society, 2016
    Co-Authors: Sunday A Malomo, Rotimi E. Aluko
    Abstract:

    The aim of this work was to characterize the structural and functional properties of Hemp Seed protein-derived acetylcholinesterase (AChE)-inhibitory enzymatic hydrolysates. Hemp Seed protein isolate hydrolysis was performed using six different proteases (pepsin, papain, thermoase, flavourzyme, alcalase and pepsin + pancreatin) at different concentrations (1–4 %). The degree of hydrolysis was directly related to the amount of protease used but had no relationship with AChE-inhibitory activity. Amino acid composition results showed that the Hemp Seed protein hydrolysates (HPHs) had high levels of negatively charged amino acids (39.62–40.18 %) as well as arginine. The 1 % pepsin HPH was the most active AChE inhibitor with ~6 µg/mL IC50 value when compared to 8–11.6 µg/mL for the other HPHs. Mass spectrometry analysis showed that most of the peptides in all the hydrolysates were less than 1000 Da in size. However, the pepsin HPHs contained larger-sized peptides (244–1009 Da) than the papain HPHs (246–758 Da), which in turn was larger than the alcalase HPH (246–607 Da). The higher AChE-inhibitory effects of the pepsin HPHs may be due to increased synergistic effects from a wider peptide size range when compared to the papain and alcalase HPHs that had narrower ranges. The narrow peptide size range in the alcalase HPH confirms the higher efficiency of this protease in releasing small-sized peptides from food proteins.

  • conversion of a low protein Hemp Seed meal into a functional protein concentrate through enzymatic digestion of fibre coupled with membrane ultrafiltration
    Innovative Food Science and Emerging Technologies, 2015
    Co-Authors: Sunday A Malomo, Rotimi E. Aluko
    Abstract:

    Abstract Industrial Hemp Seed meal (HPM) containing ~37% protein content was digested with carbohydrases and a phytase. The digest was then passed through a 10 kDa ultrafiltration membrane and the retentate freeze-dried as the membrane protein concentrate (mHPC), which had 74% protein content. Protein digestibility (89%) was significantly (P Industrial relevance Hemp Seed oil remains the most valuable product of the Hemp Seed industry but the proteins are also becoming very popular due to the high arginine content. Arginine is perceived as a heart-healthy amino acid because it serves as a precursor of the vasodilatory agent, nitric oxide. The traditional method of protein isolation involves isoelectric precipitation, which damages protein functionality and reduces performance as an ingredient below the required level for high quality food products manufacture. Therefore, in this research work, a novel, industrially scalable method that uses food-grade enzymes to digest most of the polysaccharides in a defatted low protein Hemp Seed meal was developed. The digested polysaccharide fragments were then removed by ultrafiltration/diafiltration to leave behind a protein product with twice the original protein content of the starting raw material. Most importantly, the work confirmed that the membrane-isolated protein possessed superior functionality, especially protein solubility and digestibility in comparison with existing similar Hemp Seed protein products. Since the membrane ultrafiltration setup is available on a commercial scale, work could be adapted by the Hemp Seed processing industry to manufacture a new line of high protein products with desirable functionality in food systems. However, spray-drying must be used instead of freeze-drying in order to make the process economically feasible.

  • structural and antihypertensive properties of enzymatic Hemp Seed protein hydrolysates
    Nutrients, 2015
    Co-Authors: Sunday A Malomo, Abraham T Girgih, John O Onuh, Rotimi E. Aluko
    Abstract:

    The aim of this work was to produce antihypertensive protein hydrolysates through different forms of enzymatic hydrolysis (2% pepsin, 4% pepsin, 1% alcalase, 2% alcalase, 2% papain, and 2% pepsin + pancreatin) of Hemp Seed proteins (HSP). The Hemp Seed protein hydrolysates (HPHs) were tested for in vitro inhibitions of renin and angiotensin-converting enzyme (ACE), two of the enzymes that regulate human blood pressure. The HPHs were then administered orally (200 mg/kg body weight) to spontaneously hypertensive rats and systolic blood pressure (SBP)-lowering effects measured over a 24 h period. Size exclusion chromatography mainly showed a 300–9560 Da peptide size range for the HPHs, while amino acid composition data had the 2% pepsin HPH with the highest cysteine content. Fluorescence spectroscopy revealed higher fluorescence intensities for the peptides when compared to the unhydrolyzed Hemp Seed protein. Overall, the 1% alcalase HPH was the most effective (p < 0.05) SBP-reducing agent (−32.5 ± 0.7 mmHg after 4 h), while the pepsin HPHs produced longer-lasting effects (−23.0 ± 1.4 mmHg after 24 h). We conclude that an optimized combination of the fast-acting HPH (1% alcalase) with the longer-lasting HPHs (2% and 4% pepsin) could provide daily effective SBP reductions.

Rong He - One of the best experts on this subject based on the ideXlab platform.

  • C: Food Chemistry Structural and Functional Properties of Hemp Seed Protein Products
    2020
    Co-Authors: Sunday A Malomo, Rong He, Rotimi E. Aluko
    Abstract:

    The effects of pH and protein concentration on some structural and functional properties of Hemp Seed protein isolate (HPI, 84.15% protein content) and defatted Hemp Seed protein meal (HPM, 44.32% protein content) were determined. The HPI had minimum protein solubility (PS) at pH 4.0, which increased as pH was decreased or increased. In contrast, the HPM had minimum PS at pH 3.0, which increased at higher pH values. Gel electrophoresis showed that some of the high molecular weight proteins (>45 kDa) present in HPM were not well extracted by the alkali and were absent or present in low ratio in the HPI polypeptide profile. The amino acid composition showed that the isolation process increased the Arg/Lys ratio of HPI (5.52%) when compared to HPM (3.35%). Intrinsic fluorescence and circular dichroism data indicate that the HPI proteins had a well-defined structure at pH 3.0, which was lost as pH value increased. The differences in structural conformation of HPI at different pH values were reflected as better foaming capacity at pH 3.0 when compared to pH 5.0, 7.0, and 9.0. At 10 and 25 mg/mL protein concentrations, emulsions formed by the HPM had smaller oil droplet sizes (higher quality), when compared to the HPI-formed emulsions. In contrast at 50 mg/mL protein concentration, the HPI-formed emulsions had smaller oil droplet sizes (except at pH 3.0). We conclude that the functional properties of Hemp Seed protein products are dependent on structural conformations as well as protein concentration and pH. Practical Application: Hemp Seed is a popular oil Seed crop grown in Canada and globally for its oil and protein products. Hemp Seed proteins (HSPs), which account for 25% of its composition, have been widely studied in detail, but their structural and functional properties at varying processing conditions are not well understood. This work provides information on the structural conformation and functional performance in model systems of HSP under varying pH conditions that cover the food processing range. The presented data could enhance manipulation of environmental pH conditions for the use of HSP as novel food ingredients.

  • a novel Hemp Seed meal protein hydrolysate reduces oxidative stress factors in spontaneously hypertensive rats
    Nutrients, 2014
    Co-Authors: Abraham T Girgih, Adeola Alashi, Sunday A Malomo, Rong He, Thomas Netticadan, Rotimi E. Aluko
    Abstract:

    This report shows the antioxidant effects of a Hemp Seed meal protein hydrolysate (HMH) in spontaneously hypertensive rats (SHR). Defatted Hemp Seed meal was hydrolyzed consecutively with pepsin and pancreatin to yield HMH, which was incorporated into rat feed as a source of antioxidant peptides. Young (8-week old) SHRs were divided into three groups (8 rats/group) and fed diets that contained 0.0%, 0.5% or 1.0% (w/w) HMH for eight weeks; half of the rats were sacrificed for blood collection. After a 4-week washout period, the remaining 20-week old SHRs were fed for an additional four weeks and sacrificed for blood collection. Plasma total antioxidant capacity (TAC) and superoxide dismutase (SOD), catalase (CAT) and total peroxides (TPx) levels were determined. Results showed that plasma TAC, CAT and SOD levels decreased in the older 20-week old SHRs when compared to the young SHRs. The presence of HMH in the diets led to significant (p < 0.05) increases in plasma SOD and CAT levels in both young and adult SHR groups; these increases were accompanied by decreases in TPx levels. The results suggest that HMH contained antioxidant peptides that reduced the rate of lipid peroxidation in SHRs with enhanced antioxidant enzyme levels and total antioxidant capacity.

  • Preventive and treatment effects of a Hemp Seed (Cannabis sativa L.) meal protein hydrolysate against high blood pressure in spontaneously hypertensive rats
    European Journal of Nutrition, 2014
    Co-Authors: Abraham T Girgih, Adeola Alashi, Rong He, S. Malomo, Rotimi E. Aluko
    Abstract:

    PurposeThis work determined the ability of Hemp Seed meal protein hydrolysate (HMH)-containing diets to attenuate elevated blood pressure (hypertension) development in spontaneously hypertensive rats (SHRs). Effects of diets on plasma levels of renin and angiotensin I-converting enzyme (ACE) in the SHRs were also determined.MethodsDefatted Hemp Seed protein meal was hydrolyzed using simulated gastrointestinal tract digestion with pepsin followed by pancreatin, and the resulting HMH used as a source of antihypertensive peptides. The HMH was substituted for casein at 0.5 and 1.0 % levels and fed to young growing rats for 8 weeks (preventive phase) or adult rats for 4 weeks (treatment phase).ResultsFeeding of young growing SHRs with HMH resulted in attenuation of the normal increases in systolic blood pressure (SBP) with an average value of ~120 mmHg when compared to the casein-only group of rats (control) with a maximum of 158 mm Hg (p 

  • structural and functional properties of Hemp Seed protein products
    Journal of Food Science, 2014
    Co-Authors: Sunday A Malomo, Rong He, Rotimi E. Aluko
    Abstract:

    : The effects of pH and protein concentration on some structural and functional properties of Hemp Seed protein isolate (HPI, 84.15% protein content) and defatted Hemp Seed protein meal (HPM, 44.32% protein content) were determined. The HPI had minimum protein solubility (PS) at pH 4.0, which increased as pH was decreased or increased. In contrast, the HPM had minimum PS at pH 3.0, which increased at higher pH values. Gel electrophoresis showed that some of the high molecular weight proteins (>45 kDa) present in HPM were not well extracted by the alkali and were absent or present in low ratio in the HPI polypeptide profile. The amino acid composition showed that the isolation process increased the Arg/Lys ratio of HPI (5.52%) when compared to HPM (3.35%). Intrinsic fluorescence and circular dichroism data indicate that the HPI proteins had a well-defined structure at pH 3.0, which was lost as pH value increased. The differences in structural conformation of HPI at different pH values were reflected as better foaming capacity at pH 3.0 when compared to pH 5.0, 7.0, and 9.0. At 10 and 25 mg/mL protein concentrations, emulsions formed by the HPM had smaller oil droplet sizes (higher quality), when compared to the HPI-formed emulsions. In contrast at 50 mg/mL protein concentration, the HPI-formed emulsions had smaller oil droplet sizes (except at pH 3.0). We conclude that the functional properties of Hemp Seed protein products are dependent on structural conformations as well as protein concentration and pH.

  • preventive and treatment effects of a Hemp Seed cannabis sativa l meal protein hydrolysate against high blood pressure in spontaneously hypertensive rats
    European Journal of Nutrition, 2014
    Co-Authors: Abraham T Girgih, Adeola Alashi, Sunday A Malomo, Rong He, Rotimi E. Aluko
    Abstract:

    Purpose This work determined the ability of Hemp Seed meal protein hydrolysate (HMH)-containing diets to attenuate elevated blood pressure (hypertension) development in spontaneously hypertensive rats (SHRs). Effects of diets on plasma levels of renin and angiotensin I-converting enzyme (ACE) in the SHRs were also determined.

Adeola Alashi - One of the best experts on this subject based on the ideXlab platform.

  • a novel Hemp Seed meal protein hydrolysate reduces oxidative stress factors in spontaneously hypertensive rats
    Nutrients, 2014
    Co-Authors: Abraham T Girgih, Adeola Alashi, Sunday A Malomo, Rong He, Thomas Netticadan, Rotimi E. Aluko
    Abstract:

    This report shows the antioxidant effects of a Hemp Seed meal protein hydrolysate (HMH) in spontaneously hypertensive rats (SHR). Defatted Hemp Seed meal was hydrolyzed consecutively with pepsin and pancreatin to yield HMH, which was incorporated into rat feed as a source of antioxidant peptides. Young (8-week old) SHRs were divided into three groups (8 rats/group) and fed diets that contained 0.0%, 0.5% or 1.0% (w/w) HMH for eight weeks; half of the rats were sacrificed for blood collection. After a 4-week washout period, the remaining 20-week old SHRs were fed for an additional four weeks and sacrificed for blood collection. Plasma total antioxidant capacity (TAC) and superoxide dismutase (SOD), catalase (CAT) and total peroxides (TPx) levels were determined. Results showed that plasma TAC, CAT and SOD levels decreased in the older 20-week old SHRs when compared to the young SHRs. The presence of HMH in the diets led to significant (p < 0.05) increases in plasma SOD and CAT levels in both young and adult SHR groups; these increases were accompanied by decreases in TPx levels. The results suggest that HMH contained antioxidant peptides that reduced the rate of lipid peroxidation in SHRs with enhanced antioxidant enzyme levels and total antioxidant capacity.

  • Preventive and treatment effects of a Hemp Seed (Cannabis sativa L.) meal protein hydrolysate against high blood pressure in spontaneously hypertensive rats
    European Journal of Nutrition, 2014
    Co-Authors: Abraham T Girgih, Adeola Alashi, Rong He, S. Malomo, Rotimi E. Aluko
    Abstract:

    PurposeThis work determined the ability of Hemp Seed meal protein hydrolysate (HMH)-containing diets to attenuate elevated blood pressure (hypertension) development in spontaneously hypertensive rats (SHRs). Effects of diets on plasma levels of renin and angiotensin I-converting enzyme (ACE) in the SHRs were also determined.MethodsDefatted Hemp Seed protein meal was hydrolyzed using simulated gastrointestinal tract digestion with pepsin followed by pancreatin, and the resulting HMH used as a source of antihypertensive peptides. The HMH was substituted for casein at 0.5 and 1.0 % levels and fed to young growing rats for 8 weeks (preventive phase) or adult rats for 4 weeks (treatment phase).ResultsFeeding of young growing SHRs with HMH resulted in attenuation of the normal increases in systolic blood pressure (SBP) with an average value of ~120 mmHg when compared to the casein-only group of rats (control) with a maximum of 158 mm Hg (p 

  • preventive and treatment effects of a Hemp Seed cannabis sativa l meal protein hydrolysate against high blood pressure in spontaneously hypertensive rats
    European Journal of Nutrition, 2014
    Co-Authors: Abraham T Girgih, Adeola Alashi, Sunday A Malomo, Rong He, Rotimi E. Aluko
    Abstract:

    Purpose This work determined the ability of Hemp Seed meal protein hydrolysate (HMH)-containing diets to attenuate elevated blood pressure (hypertension) development in spontaneously hypertensive rats (SHRs). Effects of diets on plasma levels of renin and angiotensin I-converting enzyme (ACE) in the SHRs were also determined.