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K Kalimuthu - One of the best experts on this subject based on the ideXlab platform.
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phytochemical evaluation and antioxidant activity of Holarrhena pubescens wall ex g don
Social Science Research Network, 2018Co-Authors: A Arunprasath, K KalimuthuAbstract:The selected medicinal plant Holarrhena pubescens. ex G.Don belongs to the family Apocynaceae, and it was collected in hillock of Muthu Malai hill in Coimbatore, Tamil Nadu. In the present study, preliminary phytochemical screening of H. pubescens a medicinal plant was carried out. Qualitative phytochemical analysis of these plants confirms the presence of various secondary metabolites such as steroids, tannins, alkaloids, and phenols. The results suggest that the phytochemical properties for curing various ailments possess potential anti-inflammatory, antimicrobial, and antioxidant and leads to the isolation of new and novel compounds. Gas chromatography-mass spectrometry analysis showed the existence of various compounds with different chemical structures. The presence of various bioactive compounds confirms the application of H. pubescens for various ailments by traditional practitioners. However, isolation of individual phytochemical constituents may proceed to find a novel drug. Extracts from H. pubescens showed varying antioxidant (free radical scavenging) activities when compared to Vitamin C, and the results suggest that the antioxidant activity of H. pubescens may contribute to their claimed medicinal property.
V S Bisaria - One of the best experts on this subject based on the ideXlab platform.
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alkaloid production by plant cell cultures of Holarrhena antidysenterica ii effect of precursor feeding and cultivation in stirred tank bioreactor
Biotechnology and Bioengineering, 1992Co-Authors: Amulya K Panda, V S Bisaria, Saroj MishraAbstract:Precursor feeding strategy for increasing the yield of conessine, a steroidal alkaloid of Holarrhena antidysenterica, was established in cell suspension culture. A total of 50 mg/L added cholesterol was converted into 43 mg/L of alkaloid, 90% of which constituted the conessine. By applying the precursor feeding policy to the cell suspension culture in modified Murashige and Skoog (MS) medium, a total of 143 mg/L of alkaloid was produced in 8 days. In this way the alkaloid content of the cells was increased more than six times compared to that obtained in the standard MS medium. The steps leading to biotransformation of cholesterol into alkaloids were unaffected by phosphate. The shake flask data were successfully transferred to a bench scale 6-L stirred tank bioreactor in which the specific biosynthetic rate of alkaloid production was 110 mg/100 g dry cell weight per day, about 160 times higher than that of whole plant.
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alkaloid production by plant cell suspension cultures of Holarrhena antidysenterica i effect of major nutrients
Biotechnology and Bioengineering, 1992Co-Authors: Amulya K Panda, Saroj Mishra, V S BisariaAbstract:The effect of major nutrients on growth and alkaloid production by plant cell culture of Holarrhena antidysenterica was studied with a view to increasing the yield of the alkaloid conessine, a therapeutic drug used for treatment of dysentery and helminthic disorders. The studies resulted in development of a modified Murashige and Skoog (MS) medium that contained 60 mM total nitrogen with a NH(4) (+)-to-NO(3) (-) ratio of 5:1, 0.25 mM phosphate, and 40 g/L sucrose. The growth regulators 2,4-dichlorophenoxy acetic acid (2,4-D) and kinetin (Kn) were also found to affect the synthesis of alkaloid. Using an optimal level of inoculum (3 g/L), the modified medium resulted in alkaloid synthesis of 0.66 g/100 g dry cell weight, which represented a 4.25-fold increase over that obtained in standard MS medium.
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cell culture of Holarrhena antidysenterica growth and alkaloid production
Phytochemistry, 1991Co-Authors: Amulya K Panda, V S Bisaria, Saroj Mishra, Sant S BhojwaniAbstract:Abstract Callus and suspension culture of Holarrhena antidysenterica were established for production of steroidal alkaloids, especially conessine. The doubling time and specific growth rate of cells in suspension culture were computed to be 47.5 hr and 0.35 per day respectively. A maximum of 300 mg alkaloids per 100 g dry cell wt in 40 days and 130 mg per 100 g dry cell wt in eight days were obtained in the callus and suspension cultures respectively. Alkaloid production in suspension culture was a combined growth and non-growth associated phenomena. About 90% of the total alkaloids produced in the cell culture was conessine, which was confirmed by spectral data.
Amulya K Panda - One of the best experts on this subject based on the ideXlab platform.
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alkaloid production by plant cell cultures of Holarrhena antidysenterica ii effect of precursor feeding and cultivation in stirred tank bioreactor
Biotechnology and Bioengineering, 1992Co-Authors: Amulya K Panda, V S Bisaria, Saroj MishraAbstract:Precursor feeding strategy for increasing the yield of conessine, a steroidal alkaloid of Holarrhena antidysenterica, was established in cell suspension culture. A total of 50 mg/L added cholesterol was converted into 43 mg/L of alkaloid, 90% of which constituted the conessine. By applying the precursor feeding policy to the cell suspension culture in modified Murashige and Skoog (MS) medium, a total of 143 mg/L of alkaloid was produced in 8 days. In this way the alkaloid content of the cells was increased more than six times compared to that obtained in the standard MS medium. The steps leading to biotransformation of cholesterol into alkaloids were unaffected by phosphate. The shake flask data were successfully transferred to a bench scale 6-L stirred tank bioreactor in which the specific biosynthetic rate of alkaloid production was 110 mg/100 g dry cell weight per day, about 160 times higher than that of whole plant.
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alkaloid production by plant cell suspension cultures of Holarrhena antidysenterica i effect of major nutrients
Biotechnology and Bioengineering, 1992Co-Authors: Amulya K Panda, Saroj Mishra, V S BisariaAbstract:The effect of major nutrients on growth and alkaloid production by plant cell culture of Holarrhena antidysenterica was studied with a view to increasing the yield of the alkaloid conessine, a therapeutic drug used for treatment of dysentery and helminthic disorders. The studies resulted in development of a modified Murashige and Skoog (MS) medium that contained 60 mM total nitrogen with a NH(4) (+)-to-NO(3) (-) ratio of 5:1, 0.25 mM phosphate, and 40 g/L sucrose. The growth regulators 2,4-dichlorophenoxy acetic acid (2,4-D) and kinetin (Kn) were also found to affect the synthesis of alkaloid. Using an optimal level of inoculum (3 g/L), the modified medium resulted in alkaloid synthesis of 0.66 g/100 g dry cell weight, which represented a 4.25-fold increase over that obtained in standard MS medium.
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cell culture of Holarrhena antidysenterica growth and alkaloid production
Phytochemistry, 1991Co-Authors: Amulya K Panda, V S Bisaria, Saroj Mishra, Sant S BhojwaniAbstract:Abstract Callus and suspension culture of Holarrhena antidysenterica were established for production of steroidal alkaloids, especially conessine. The doubling time and specific growth rate of cells in suspension culture were computed to be 47.5 hr and 0.35 per day respectively. A maximum of 300 mg alkaloids per 100 g dry cell wt in 40 days and 130 mg per 100 g dry cell wt in eight days were obtained in the callus and suspension cultures respectively. Alkaloid production in suspension culture was a combined growth and non-growth associated phenomena. About 90% of the total alkaloids produced in the cell culture was conessine, which was confirmed by spectral data.
Saroj Mishra - One of the best experts on this subject based on the ideXlab platform.
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alkaloid production by plant cell cultures of Holarrhena antidysenterica ii effect of precursor feeding and cultivation in stirred tank bioreactor
Biotechnology and Bioengineering, 1992Co-Authors: Amulya K Panda, V S Bisaria, Saroj MishraAbstract:Precursor feeding strategy for increasing the yield of conessine, a steroidal alkaloid of Holarrhena antidysenterica, was established in cell suspension culture. A total of 50 mg/L added cholesterol was converted into 43 mg/L of alkaloid, 90% of which constituted the conessine. By applying the precursor feeding policy to the cell suspension culture in modified Murashige and Skoog (MS) medium, a total of 143 mg/L of alkaloid was produced in 8 days. In this way the alkaloid content of the cells was increased more than six times compared to that obtained in the standard MS medium. The steps leading to biotransformation of cholesterol into alkaloids were unaffected by phosphate. The shake flask data were successfully transferred to a bench scale 6-L stirred tank bioreactor in which the specific biosynthetic rate of alkaloid production was 110 mg/100 g dry cell weight per day, about 160 times higher than that of whole plant.
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alkaloid production by plant cell suspension cultures of Holarrhena antidysenterica i effect of major nutrients
Biotechnology and Bioengineering, 1992Co-Authors: Amulya K Panda, Saroj Mishra, V S BisariaAbstract:The effect of major nutrients on growth and alkaloid production by plant cell culture of Holarrhena antidysenterica was studied with a view to increasing the yield of the alkaloid conessine, a therapeutic drug used for treatment of dysentery and helminthic disorders. The studies resulted in development of a modified Murashige and Skoog (MS) medium that contained 60 mM total nitrogen with a NH(4) (+)-to-NO(3) (-) ratio of 5:1, 0.25 mM phosphate, and 40 g/L sucrose. The growth regulators 2,4-dichlorophenoxy acetic acid (2,4-D) and kinetin (Kn) were also found to affect the synthesis of alkaloid. Using an optimal level of inoculum (3 g/L), the modified medium resulted in alkaloid synthesis of 0.66 g/100 g dry cell weight, which represented a 4.25-fold increase over that obtained in standard MS medium.
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cell culture of Holarrhena antidysenterica growth and alkaloid production
Phytochemistry, 1991Co-Authors: Amulya K Panda, V S Bisaria, Saroj Mishra, Sant S BhojwaniAbstract:Abstract Callus and suspension culture of Holarrhena antidysenterica were established for production of steroidal alkaloids, especially conessine. The doubling time and specific growth rate of cells in suspension culture were computed to be 47.5 hr and 0.35 per day respectively. A maximum of 300 mg alkaloids per 100 g dry cell wt in 40 days and 130 mg per 100 g dry cell wt in eight days were obtained in the callus and suspension cultures respectively. Alkaloid production in suspension culture was a combined growth and non-growth associated phenomena. About 90% of the total alkaloids produced in the cell culture was conessine, which was confirmed by spectral data.
Walter Luyten - One of the best experts on this subject based on the ideXlab platform.
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metabolic diversity and therapeutic potential of Holarrhena pubescens an important ethnomedicinal plant
Biomolecules, 2020Co-Authors: Kulsoom Zahara, Sujogya Kumar Panda, Shasank S Swain, Walter LuytenAbstract:Holarrhena pubescens is an important medicinal plant of the Apocynaceae family that is widely distributed over the Indian subcontinent. The plant is extensively used in Ayurveda and other traditional medicinal systems without obvious adverse effects. Beside notable progress in the biological and phytochemical evaluation of this plant over the past few years, comprehensive reviews of H. pubescens are limited in scope. It has economic importance due to the extensive use of seeds as an antidiabetic. Furthermore, the plant is extensively reported in traditional uses among the natives of Asia and Africa, while scientifical validation for various ailments has not been studied either in vitro or in vivo. This review aims to summarize information on the pharmacology, traditional uses, active constituents, safety and toxicity of H. pubescens. Chemical analysis of H. pubescens extracts revealed the presence of several bioactive compounds, such as conessine, isoconnessine, conessimine, conimine, conessidine, conkurchicine, holarrhimine, conarrhimine, mokluangin A-D and antidysentericine. Overall, this review covers the ethnopharmacology, phytochemical composition, and pharmacological potential of H. pubescens, with a critical discussion of its toxicity, biological activities (in vitro and in vivo), the mechanism of action, as well as suggestions for further basic and clinical research.