The Experts below are selected from a list of 192 Experts worldwide ranked by ideXlab platform
A J Ainsworth - One of the best experts on this subject based on the ideXlab platform.
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glucan administration potentiates immune defence mechanisms of channel catfish Ictalurus punctatus rafinesque
Journal of Fish Diseases, 1992Co-Authors: D Chen, A J AinsworthAbstract:. Intraperitoncal injection of β-l,3 glucan in channel catfish, Ictalurus punctatus Rafinesque, greatly reduces mortality from experimental infection with Edwardsiella ictaluri. Anterior kidney phagocytes from fish receiving glucan had enhanced phagocytic and bactericidal ability. The elevated bactericidal ability of phagocytes was not accompanied by increased production of hydrogen peroxide. Fish injected with glucan responded to subsequent E. ictaluri immunizations with higher serum antibody titres relative to the control catfish. The timing of glucan administration and antigen immunization was also important. These results indicate that β-1,3 glucan potentially could be utilized prophylactically as an immunomodulator in channel catfish.
P.j. Bentley - One of the best experts on this subject based on the ideXlab platform.
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Influx of zinc by channel catfish (Ictalurus punctatus): Uptake from external environmental solutions
Comparative Biochemistry and Physiology Part C: Comparative Pharmacology, 1992Co-Authors: P.j. BentleyAbstract:Abstract 1. Channel catfish ( Ictalurus punctatus ) take up zinc (measured with 65 Zn) from external ambient solutions in a concentration dependent manner. At a concentration of 10 −6 M Zn, this uptake is equivalent to 0.4% of the total body Zn each day. 2. Zinc influx was increased by external acid conditions (decreasing pH from 7.3 to 5). 3. Elevated Ca 2+ and Cd 2+ , but not Al 3+ , concentrations markedly decreased the uptake of Zn. 4. These observations may be relevant to circumstances that occur under natural conditions, and influence the zinc nutrition and toxicity of the fish.
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Some observations on the calcium metabolism of channel catfish (Ictalurus punctatus)
Comparative Biochemistry and Physiology Part A: Physiology, 1992Co-Authors: P.j. BentleyAbstract:Abstract 1. 1. Calcium concentrations in the serum and body tissues of channel catfish (Ictalurus punctatus) are described. 2. 2. About 45% of the Ca in serum was bound to serum proteins. The albumin fraction had a higher binding capacity than whole serum. The concentration dependence of the binding suggested the presence of high- and low-affinity sites. Binding of Ca to serum proteins may not only help maintain its “free” concentrations in plasma, but also limit diffusional losses across the integument. 3. 3. An influx of Ca (as 45Ca) takes place across the integument of catfish. The possible physiological significance of this uptake is discussed.
Phillip H Klesius - One of the best experts on this subject based on the ideXlab platform.
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flavobacterium columnare genomovar influences mortality in channel catfish Ictalurus punctatus
Veterinary Microbiology, 2008Co-Authors: Craig A Shoemaker, Oscar Olivaresfuster, Covadonga R. Arias, Phillip H KlesiusAbstract:Abstract Flavobacterium columnare, causal agent of columnaris disease, is pathogenic to many species of freshwater fish throughout the world. The United States channel catfish (Ictalurus punctatus) aquaculture industry is severely impacted by columnaris disease. The majority of the F. columnare isolates recovered from diseased channel catfish belonged to either genomovars I or II. The objective of the present study was to determine if differences existed in the ability of these genomovars to induce mortality in channel catfish. Single strand conformation polymorphism analysis (SSCP) was used to ascribe the isolates used in this study to the appropriate genomovar. Immersion challenge experiments (15 min immersion exposure to ∼5 × 105 to 1 × 106 CFU/mL) were carried out to assess virulence of genomovar I and II isolates to channel catfish. The results demonstrated that genomovar II (n = 4) isolates were significantly (P
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Flavobacterium columnare genomovar influences mortality in channel catfish (Ictalurus punctatus).
Veterinary microbiology, 2007Co-Authors: Craig A Shoemaker, Oscar Olivares-fuster, Covadonga R. Arias, Phillip H KlesiusAbstract:Flavobacterium columnare, causal agent of columnaris disease, is pathogenic to many species of freshwater fish throughout the world. The United States channel catfish (Ictalurus punctatus) aquaculture industry is severely impacted by columnaris disease. The majority of the F. columnare isolates recovered from diseased channel catfish belonged to either genomovars I or II. The objective of the present study was to determine if differences existed in the ability of these genomovars to induce mortality in channel catfish. Single strand conformation polymorphism analysis (SSCP) was used to ascribe the isolates used in this study to the appropriate genomovar. Immersion challenge experiments (15min immersion exposure to approximately 5x10(5) to 1x10(6) CFU/mL) were carried out to assess virulence of genomovar I and II isolates to channel catfish. The results demonstrated that genomovar II (n=4) isolates were significantly (P
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Development of an Indirect ELISA to Detect Humoral Response to Flavobacterium columnare Infection of Channel Catfish, Ictalurus punctatus
Journal of Applied Aquaculture, 2003Co-Authors: Craig A Shoemaker, Richard A. Shelby, Phillip H KlesiusAbstract:ABSTRACT The humoral antibody response of channel catfish, Ictalurus punctatus, to experimental Flavobacterium columnare infection was measured in control (non-infected) and infected (intraperitoneal- and intramuscular-injected) fish by an indirect enzyme-linked immunoassay (ELISA). The antibody response of the experimentally infected fish was significantly higher (P
D Chen - One of the best experts on this subject based on the ideXlab platform.
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glucan administration potentiates immune defence mechanisms of channel catfish Ictalurus punctatus rafinesque
Journal of Fish Diseases, 1992Co-Authors: D Chen, A J AinsworthAbstract:. Intraperitoncal injection of β-l,3 glucan in channel catfish, Ictalurus punctatus Rafinesque, greatly reduces mortality from experimental infection with Edwardsiella ictaluri. Anterior kidney phagocytes from fish receiving glucan had enhanced phagocytic and bactericidal ability. The elevated bactericidal ability of phagocytes was not accompanied by increased production of hydrogen peroxide. Fish injected with glucan responded to subsequent E. ictaluri immunizations with higher serum antibody titres relative to the control catfish. The timing of glucan administration and antigen immunization was also important. These results indicate that β-1,3 glucan potentially could be utilized prophylactically as an immunomodulator in channel catfish.
Huseyin Kucuktas - One of the best experts on this subject based on the ideXlab platform.
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enhanced bacterial disease resistance of transgenic channel catfish Ictalurus punctatus possessing cecropin genes
Marine Biotechnology, 2002Co-Authors: Rex A Dunham, Gregory W Warr, Amy Nichols, Patricia L Duncan, Brad J Argue, Darlene L Middleton, Huseyin KucuktasAbstract:The cecropin B gene from the moth Hyalophora cecropia, driven by the cytomegalovirus promoter, was transferred to the channel catfish Ictalurus punctatus. Transgenic individuals (P1) were mated to produce individuals (F1) that exhibited enhanced disease resistance and survival when challenged with pathogenic bacteria. During the epizootic of Flavobacterium columnare in an earthen pond, the percentage of transgenic individuals containing preprocecropin B construct that survived (100%) was significantly greater (P 0.05). Inheritance of the transgene by the F1 generation, 20.2% to 30.7% was typical of that in studies with transgenic channel catfish.
