The Experts below are selected from a list of 219 Experts worldwide ranked by ideXlab platform
Babu Varghese - One of the best experts on this subject based on the ideXlab platform.
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partial Regeneration of β cells in the islets of langerhans by nymphayol a sterol isolated from nymphaea stellata willd flowers
Bioorganic & Medicinal Chemistry, 2009Co-Authors: P Subashbabu, S Ignacimuthu, P Agastian, Babu VargheseAbstract:Reduction of the β-cell mass is critical in the pathogenesis of diabetes mellitus. The discovery of agents which Induce Regeneration of pancreatic β-cells would be useful to develop new therapeutic approaches to treat diabetes. The present study was aimed at identifying a new agent for the control of diabetes through Regeneration of pancreatic β cells and insulin secretory potential. Nymphaea stellata flower chloroform extract ( NSFCExt ) showed significant plasma glucose lowering effect. Further NSFCExt was utilized to isolate and identify the lead compound based on bioassay guided fractionation; we found Nymphayol (25,26-dinorcholest-5-en-3β-ol) a new crystal [space group P 2 1 (No. 4), a = 9.618(5), b = 7.518(5), c = 37.491(5)]. It was purified by repeat column. The structure was determined on the basis of X-ray crystallography and spectral data. Oral administration of Nymphayol for 45 days significantly ( p < 0.05) lowered the blood glucose level and more importantly it effectively increased the insulin content in diabetic rats. In addition, Nymphayol increased the number of β cell mass enormously. Islet-like cell clusters in the islets of Langerhans were clearly observed based on histochemical and immunohistochemical study.
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partial Regeneration of beta cells in the islets of langerhans by nymphayol a sterol isolated from nymphaea stellata willd flowers
Bioorganic & Medicinal Chemistry, 2009Co-Authors: P Subashbabu, S Ignacimuthu, P Agastian, Babu VargheseAbstract:Reduction of the beta-cell mass is critical in the pathogenesis of diabetes mellitus. The discovery of agents which Induce Regeneration of pancreatic beta-cells would be useful to develop new therapeutic approaches to treat diabetes. The present study was aimed at identifying a new agent for the control of diabetes through Regeneration of pancreatic beta cells and insulin secretory potential. Nymphaea stellata flower chloroform extract (NSFCExt) showed significant plasma glucose lowering effect. Further NSFCExt was utilized to isolate and identify the lead compound based on bioassay guided fractionation; we found Nymphayol (25,26-dinorcholest-5-en-3beta-ol) a new crystal [space group P2(1) (No. 4), a=9.618(5), b=7.518(5), c=37.491(5)]. It was purified by repeat column. The structure was determined on the basis of X-ray crystallography and spectral data. Oral administration of Nymphayol for 45 days significantly (p<0.05) lowered the blood glucose level and more importantly it effectively increased the insulin content in diabetic rats. In addition, Nymphayol increased the number of beta cell mass enormously. Islet-like cell clusters in the islets of Langerhans were clearly observed based on histochemical and immunohistochemical study.
Roberto P Revoltella - One of the best experts on this subject based on the ideXlab platform.
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transplanted human adipose tissue derived stem cells engraft and Induce Regeneration in mice olfactory neuroepithelium in response to dichlobenil subministration
Chemical Senses, 2014Co-Authors: Valeria Franceschini, Simone Bettini, Simone Pifferi, Anna Menini, Gabriele Siciliano, Emanuela Ognio, A T Brini, Roberto P RevoltellaAbstract:: We used immunodeficient mice, whose dorsomedial olfactory region was permanently damaged by dichlobenil inoculation, to test the neuroregenerative properties of transplanted human adipose tissue-derived stem cells after 30 and 60 days. Analysis of polymerase chain reaction bands revealed that stem cells preferentially engrafted in the lesioned olfactory epithelium compared with undamaged mucosa of untreated transplanted mice. Although basal cell proliferation in untransplanted lesioned mice did not give rise to neuronal cells in the olfactory mucosa, we observed clusters of differentiating olfactory cells in transplanted mice. After 30 days, and even more at 60 days, epithelial thickness was partially recovered to normal values, as also the immunohistochemical properties. Functional reactivity to odorant stimulation was also confirmed through electro-olfactogram recording in the dorsomedial epithelium. Furthermore, we demonstrated that engrafted stem cells fused with mouse cells in the olfactory organ, even if heterokaryons detected were too rare to hypothesize they directly repopulated the lesioned epithelium. The data reported prove that the migrating transplanted stem cells were able to Induce a neuroregenerative process in a specific lesioned sensory area, enforcing the perspective that they could become an available tool for stem cell therapy.
P Subashbabu - One of the best experts on this subject based on the ideXlab platform.
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partial Regeneration of β cells in the islets of langerhans by nymphayol a sterol isolated from nymphaea stellata willd flowers
Bioorganic & Medicinal Chemistry, 2009Co-Authors: P Subashbabu, S Ignacimuthu, P Agastian, Babu VargheseAbstract:Reduction of the β-cell mass is critical in the pathogenesis of diabetes mellitus. The discovery of agents which Induce Regeneration of pancreatic β-cells would be useful to develop new therapeutic approaches to treat diabetes. The present study was aimed at identifying a new agent for the control of diabetes through Regeneration of pancreatic β cells and insulin secretory potential. Nymphaea stellata flower chloroform extract ( NSFCExt ) showed significant plasma glucose lowering effect. Further NSFCExt was utilized to isolate and identify the lead compound based on bioassay guided fractionation; we found Nymphayol (25,26-dinorcholest-5-en-3β-ol) a new crystal [space group P 2 1 (No. 4), a = 9.618(5), b = 7.518(5), c = 37.491(5)]. It was purified by repeat column. The structure was determined on the basis of X-ray crystallography and spectral data. Oral administration of Nymphayol for 45 days significantly ( p < 0.05) lowered the blood glucose level and more importantly it effectively increased the insulin content in diabetic rats. In addition, Nymphayol increased the number of β cell mass enormously. Islet-like cell clusters in the islets of Langerhans were clearly observed based on histochemical and immunohistochemical study.
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partial Regeneration of beta cells in the islets of langerhans by nymphayol a sterol isolated from nymphaea stellata willd flowers
Bioorganic & Medicinal Chemistry, 2009Co-Authors: P Subashbabu, S Ignacimuthu, P Agastian, Babu VargheseAbstract:Reduction of the beta-cell mass is critical in the pathogenesis of diabetes mellitus. The discovery of agents which Induce Regeneration of pancreatic beta-cells would be useful to develop new therapeutic approaches to treat diabetes. The present study was aimed at identifying a new agent for the control of diabetes through Regeneration of pancreatic beta cells and insulin secretory potential. Nymphaea stellata flower chloroform extract (NSFCExt) showed significant plasma glucose lowering effect. Further NSFCExt was utilized to isolate and identify the lead compound based on bioassay guided fractionation; we found Nymphayol (25,26-dinorcholest-5-en-3beta-ol) a new crystal [space group P2(1) (No. 4), a=9.618(5), b=7.518(5), c=37.491(5)]. It was purified by repeat column. The structure was determined on the basis of X-ray crystallography and spectral data. Oral administration of Nymphayol for 45 days significantly (p<0.05) lowered the blood glucose level and more importantly it effectively increased the insulin content in diabetic rats. In addition, Nymphayol increased the number of beta cell mass enormously. Islet-like cell clusters in the islets of Langerhans were clearly observed based on histochemical and immunohistochemical study.
Praveen K Saxena - One of the best experts on this subject based on the ideXlab platform.
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the morphoregulatory role of thidiazuron metabolomics guided hypothesis generation for mechanisms of activity
Biomolecules, 2020Co-Authors: Lauren A E Erland, J M R Victor, Susan J Murch, Ryland T Giebelhaus, Praveen K SaxenaAbstract:Thidiazuron (TDZ) is a diphenylurea synthetic herbicide and plant growth regulator used to defoliate cotton crops and to Induce Regeneration of recalcitrant species in plant tissue culture. In vitro cultures of African violet thin petiole sections are an ideal model system for studies of TDZ-Induced morphogenesis. TDZ Induces de novo shoot organogenesis at low concentrations and somatic embryogenesis at higher concentrations of exposure. We used an untargeted metabolomics approach to identify metabolites in control and TDZ-treated tissues. Statistical analysis including metabolite clustering, pattern and pathway tools, logical algorithms, synthetic biotransformations and hormonomics identified TDZ-Induced changes in metabolism. A total of 18,602 putative metabolites with extracted masses and predicted formulae were identified with 1412 features that were found only in TDZ-treated tissues and 312 that increased in response to TDZ. The monomer of TDZ was not detected intact in the tissues but putative oligomers were found in the database and we hypothesize that these may form by a Diels–Alder reaction. Accumulation oligomers in the tissue may act as a reservoir, slowly releasing the active TDZ monomer over time. Cleavage of the amide bridge released TDZ-metabolites into the tissues including organic nitrogen and sulfur containing compounds. Metabolomics data analysis generated six novel hypotheses that can be summarized as an overall increase in uptake of sugars from the culture media, increase in primary metabolism, redirection of terpene metabolism and mediation of stress metabolism via indoleamine and phenylpropanoid metabolism. Further research into the specific mechanisms hypothesized is likely to unravel the mode of action of TDZ and to provide new insights into the control of plant morphogenesis.
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somatic embryogenesis and organogenesis in peanut the role of thidiazuron and n6 benzylaminopurine in the induction of plant morphogenesis
Plant Growth Regulation, 1999Co-Authors: J M R Victor, Susan J Murch, Sankaran Krishnaraj, Praveen K SaxenaAbstract:Intact peanut (Arachis hypogaea L.) seeds, incubated on media containing N6-benzylaminopurine (BAP) or thidiazuron (TDZ) exhibited de novo Regeneration at the hypocotyledonary notch region. Regeneration was observed when seeds were cultured on either TDZ or BAP but the optimal level of media supplementation was 10 μmol·L−1 for TDZ and 50 μmol−L−1 for BAP. Light microscopic observations revealed that the regenerants Induced by TDZ were somatic embryos while those Induced by BAP were shoots. An alternative approach of exposing the seeds to TDZ was through vacuum infiltration followed by culture on basal media but BAP did not Induce Regeneration by this method. Although TDZ has often been classified as a synthetic cytokinin, our results clearly demonstrate that seedlings treated with TDZ undergo a different morphological route of development than that Induced by purine cytokinins.
Valeria Franceschini - One of the best experts on this subject based on the ideXlab platform.
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transplanted human adipose tissue derived stem cells engraft and Induce Regeneration in mice olfactory neuroepithelium in response to dichlobenil subministration
Chemical Senses, 2014Co-Authors: Valeria Franceschini, Simone Bettini, Simone Pifferi, Anna Menini, Gabriele Siciliano, Emanuela Ognio, A T Brini, Roberto P RevoltellaAbstract:: We used immunodeficient mice, whose dorsomedial olfactory region was permanently damaged by dichlobenil inoculation, to test the neuroregenerative properties of transplanted human adipose tissue-derived stem cells after 30 and 60 days. Analysis of polymerase chain reaction bands revealed that stem cells preferentially engrafted in the lesioned olfactory epithelium compared with undamaged mucosa of untreated transplanted mice. Although basal cell proliferation in untransplanted lesioned mice did not give rise to neuronal cells in the olfactory mucosa, we observed clusters of differentiating olfactory cells in transplanted mice. After 30 days, and even more at 60 days, epithelial thickness was partially recovered to normal values, as also the immunohistochemical properties. Functional reactivity to odorant stimulation was also confirmed through electro-olfactogram recording in the dorsomedial epithelium. Furthermore, we demonstrated that engrafted stem cells fused with mouse cells in the olfactory organ, even if heterokaryons detected were too rare to hypothesize they directly repopulated the lesioned epithelium. The data reported prove that the migrating transplanted stem cells were able to Induce a neuroregenerative process in a specific lesioned sensory area, enforcing the perspective that they could become an available tool for stem cell therapy.