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Rosane Freitas Schwan - One of the best experts on this subject based on the ideXlab platform.
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effects of propionic acid and Lactobacillus buchneri ufla sil 72 addition on fermentative and microbiological characteristics of sugar cane silage treated with and without calcium oxide
Grass and Forage Science, 2012Co-Authors: B F Carvalho, C.l.s. Ávila, Jose Cardoso Pinto, M N Pereira, Rosane Freitas SchwanAbstract:The objective of this work was to evaluate the effects of a new strain of Lactobacillus buchneri (UFLA SIL 72) isolated from sugar cane (Saccharum spp.) silage and the addition of propionic acid [1% based on fresh matter (FM)] to silages treated with and without calcium oxide (1% of FM) at 60 and 170 d of ensiling. A randomized block design with a 2 × 2 × 2 × 2 factorial arrangement of treatments was used to analyse the results. The use of calcium oxide reduced the ethanol content and neutral detergent fibre in all silages, increased pH values and favoured the growth of clostridia and yeasts. The addition of propionic acid reduced the yeast population, but it was not able to reduce ethanol content of silage. The addition of L. buchneri resulted in silages with higher concentration of propionate, reduced the levels of ethanol and reduced the population of clostridia in all silages. The use of calcium oxide is not recommended for silage of sugar cane.
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effects of an indigenous and a commercial Lactobacillus buchneri strain on quality of sugar cane silage
Grass and Forage Science, 2009Co-Authors: C.l.s. Ávila, Jose Cardoso Pinto, Henrique Cesar Pereira Figueiredo, Rosane Freitas SchwanAbstract:The aim was to evaluate the effects of adding a novel Lactobacillus buchneri strain and a commercial inoculant on the fermentation and aerobic stability of sugar cane silages (Saccharum spp.). In the first experiment samples were collected from sugar cane silage at 5, 20, 40 and 80 d after ensilage in experimental silos and microorganisms belonging to the Lactobacillus genus were isolated and identified, with a wild strain of L. buchneri, UFLA SIL 72, being selected as an inoculant. In the second experiment sugar cane was inoculated with either the novel bacteria or a commercial inoculant at the moment of ensiling and compared with a control silage prepared without an inoculant. Experimental silos were opened at 0, 3, 10, 30, 60 and 90 d of ensilage and their chemical composition measured. The silages opened after 90 d were also assessed for aerobic stability. The addition of L. buchneri resulted in a higher concentration of acetic acid and reduced populations of yeasts in silage compared to the other silage treatments, and a lower ethanol concentration in the silage. The novel L. buchneri isolate and the commercial inoculant also improved aerobic stability of the sugar cane silages. It was concluded that the addition of the novel inoculants L. buchneri UFLA SIL 72 to sugar cane silage can be recommended.
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estabilidade aerobia de silagens de capim mombaca tratadas com Lactobacillus buchneri
Revista Brasileira De Zootecnia, 2009Co-Authors: C.l.s. Ávila, Odilon Gomes Pereira, Jose Cardoso Pinto, Henrique Cesar Pereira Figueiredo, Augusto Ramalho De Morais, Rosane Freitas SchwanAbstract:Objetivou-se avaliar o efeito da adicao de duas cepas de Lactobacillus buchneri sobre a estabilidade aerobia de silagens de capim-mombaca. O experimento foi conduzido em delineamento inteiramente casualizado com tres repeticoes, em esquema de parcelas subdivididas no tempo, de modo que os tratamentos (silagens sem inoculantes e com inoculante experimental e comercial) foram aleatorizados nas parcelas e os tempos de avaliacao aerobia das silagens (0, 2, 8 ou 10 dias apos a abertura dos silos) nas subparcelas. Apos 90 dias de armazenamento, os silos foram abertos e amostras de aproximadamente 2,5 kg foram retiradas para avaliacao da estabilidade aerobia com base na temperatura de cada amostra. As mudancas quimicas e microbiologicas foram avaliadas. Apos abertura dos silos, verificou-se aumento nas populacoes de bacterias do acido latico, leveduras e fungos filamentosos. Houve aumento nos valores de pH resultante da reducao da concentracao dos acidos orgnicos e de aumentos dos teores de materia seca e proteina bruta. A adicao de L. buchneri as silagens melhorou sua estabilidade, enquanto a silagem sem inoculante apresentou estabilidade de 55 horas. As silagens inoculadas nao ultrapassaram essa temperatura limite em um periodo de 10 dias. A melhoria da estabilidade foi ocasionada pela associacao de altas concentracoes de cido acetico ao acido propionico e pela menor concentracao de acido latico no momento de abertura, o que inibiu a proliferacao de fungos filamentosos e leveduras apos abertura dos silos.
L Kung - One of the best experts on this subject based on the ideXlab platform.
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effects of combining Lactobacillus buchneri 40788 with various lactic acid bacteria on the fermentation and aerobic stability of corn silage
Animal Feed Science and Technology, 2010Co-Authors: Lindsey J Reich, L KungAbstract:The objective was to compare the effectiveness of combining Lactobacillus buchneri 40788 with three lactic acid bacteria on the fermentation, aerobic stability and nutritive value of corn silage. Freshly chopped whole plant corn was untreated or treated with L. buchneri 40788 paired with Lactobacillus plantarum, Pediococcus acidilactici ,o rPediococcus pentosaceus. L. buchneri was added to achieve 4 × 105 CFU/g of fresh forage and the homolactic
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the development of lactic acid bacteria and Lactobacillus buchneri and their effects on the fermentation of alfalfa silage
Journal of Dairy Science, 2009Co-Authors: R J Schmidt, W Hu, Jason A Mills, L KungAbstract:Abstract This study was conducted to document the development of populations of lactic acid bacteria (LAB) and Lactobacillus buchneri in alfalfa silage treated with various inoculants. Wilted and chopped alfalfa (45% dry matter) was treated with 1) distilled water (untreated, U), 2) Lactobacillus buchneri 40788 (4×10 5 cfu/g; LB), or 3) L. buchneri 40788 (4×10 5 cfu/g) and Pediococcus pentosaceus (1×10 5 cfu/g; LBPP). Forages were packed into triplicate vacuum-sealed, nylon-polyethylene bags per treatment, and ensiled for 2, 5, 45, 90, and 180 d. Viable (cfu) LAB in forage and silage were quantified by traditional plating on selective agar, and numbers of L. buchneri (cfu-equivalent, cfu-E) were quantified by real-time quantitative PCR. Fresh, untreated forage had 5.52 log cfu of LAB/g and 3.79 log cfu-E of L. buchneri /g. After 2 d of ensiling, numbers of LAB increased to >8 log cfu/g in all silages. In contrast, numbers of L. buchneri in U remained below 4 log cfu-E/g but reached approximately 7 log cfu-E/g in LB and LBPP. From d 5 onward, numbers of L. buchneri in U remained below 6 log cfu-E/g but approached 9 log cfu-E/g in LB and LBPP. The pH was lower in LBPP compared with U and LB after 2 and 5 d of ensiling, but pH was lower for U compared with LB and LBPP thereafter. Treatments LB and LBPP had more acetic acid than U at 45 d of ensiling, which coincided with detectable amounts of 1,2 propanediol. Inoculation with LBPP resulted in silage with the highest concentration of 1,2 propanediol after 180 d of ensiling. From d 45 onward, LB and LBPP silages had lower concentrations of residual water-soluble carbohydrates but had higher concentrations of ammonia-N than U. In conclusion, epiphytic L. buchneri can be detected in alfalfa but this population is unable to lead the silage fermentation. In contrast, when L. buchneri was added to silage as an inoculant, the numbers of L. buchneri (cfu-E) increased markedly but did not dictate fermentation until 45 d of ensiling. These findings help to explain why the response (in increased acetic acid) from the addition of L. buchneri in silages is not immediate.
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the use of a quantitative real time polymerase chain reaction assay for identification and enumeration of Lactobacillus buchneri in silage
Journal of Applied Microbiology, 2008Co-Authors: R J Schmidt, M G Emara, L KungAbstract:Aims: To detect and quantify Lactobacillus buchneri in plant samples with the aid of polymerase chain reaction (PCR) methods. Methods and Results: DNA from silage samples spiked with different amounts of L. buchneri cells was isolated using a lysozyme/sodium dodecyl sulfate lysis and phenol/chloroform extraction method. The DNA served as a template for PCR amplification with primers specific for the bacterium. The primers were developed by comparison of 16S rDNA sequences from different lactic acid bacteria (LAB) and testing for specificity with 11 different strains of LAB. As few as 100 L. buchneri colony-forming units per gram of silage could be detected. Additionally, the technique was successfully applied to quantify the population of L. buchneri in two cultivars of corn with or without inoculation. Conclusions: The PCR assay provided a specific and rapid tool for identifying and enumerating L. buchneri in silage samples. Significance and Impact of the Study: The use of microbial inoculants for silage production is a safe and environment friendly practice, but the full potential of such additives can only be achieved with a better understanding of the fate and activity of the microbes involved. The current study describes a methodology to detect and enumerate L. buchneri, a micro-organism used as an inoculant.
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the effects of Lactobacillus buchneri 40788 and pediococcus pentosaceus r1094 on the fermentation of corn silage
Journal of Dairy Science, 2006Co-Authors: D H Kleinschmit, L KungAbstract:Abstract The effect of inoculating whole-plant corn at the time of harvest with Lactobacillus buchneri 40788 (4×10 5 cfu/g of fresh forage) combined with Pediococcus pentosaceus R1094 (1×10 5 cfu/g) on the fermentation and aerobic stability of corn silage (37% dry matter) through 361 d of ensiling was investigated. Dry matter recovery was similar between treatments throughout the study except at one early time point (14 d), when treated silage had a lower recovery than untreated silage. The concentration of lactic acid was unaffected by inoculation but inoculated silages had greater concentrations of 1,2-propanediol and acetic acid from 56 to 361 d of storage. In general, inoculation decreased the concentration of water-soluble carbohydrates but increased the concentration of ethanol. The numbers of yeasts was lower in inoculated silage at 42, 56, 70, and 282 d of ensiling. However, inoculation did not consistently improve the aerobic stability of silage, suggesting that microbes other than yeasts may have been responsible for aerobic instability in this study. Even after prolonged storage (361 d), silage treated with L. buchneri 40788 and P. pentosaceus R1094 had normal silage fermentation characteristics.
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effect of Lactobacillus buchneri 40788 on the fermentation aerobic stability and nutritive value of maize silage
Grass and Forage Science, 2002Co-Authors: N K Ranjit, C C Taylor, L KungAbstract:The effect of adding Lactobacillus buchneri 40788, a heterofermentative lactobacilli, to whole-plant maize and its effect on fermentation and aerobic stability of the resulting silage were evaluated. Whole-plant maize (380 g DM kg–1 fresh weight) was ensiled in laboratory silos after the following treatments: untreated, L. buchneri 40788 at 1 × 105, 2·5 × 105, 5 × 105, and 1 × 106 colony forming units g–1 of fresh forage or, a commercial inoculant containing homolactic acid bacteria, a single species of propionibacteria and enzymes. Addition of L. buchneri 40788 resulted in a decrease in lactic acid concentration but increased the concentration of acetic acid in silage and markedly decreased the numbers of yeasts present in the silage. All levels of L. buchneri 40788, increased the aerobic stability of silages but the effect was greatest for the two highest levels of inoculation. Treatment with the commercial inoculant had no effect on the fermentation or aerobic stability of silage. On-farm treatment of maize silage with L. buchneri 40788, stored in a bag silo, resulted in changes in fermentation that were similar to those observed in laboratory silos. Treatment decreased the numbers of yeast in silage and increased aerobic stability (+25 h) of the silage when exposed to air. Treatment had no effect on the dry-matter intake of silage fed to sheep. These results showed that treating silage with L. buchneri 40788 can increase the aerobic stability of maize silage in laboratory and farm silos.
C.l.s. Ávila - One of the best experts on this subject based on the ideXlab platform.
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Meta‐analysis of the effects of Lactobacillus plantarum and Lactobacillus buchneri on fermentation, chemical composition and aerobic stability of sugarcane silage
Grassland Science, 2018Co-Authors: Carlos Henrique Silveira Rabelo, Carla Joice Härter, C.l.s. Ávila, Ricardo Andrade ReisAbstract:We conducted a meta‐analysis to investigate the impact of Lactobacillus plantarum or Lactobacillus buchneri on the fermentation, chemical composition, and aerobic stability of sugarcane silage. Two databases that included 15 and 27 published articles (81 and 83 silages evaluated) were used to evaluate L. plantarum and L. buchneri, respectively. For each database, the means of the treatments compiled from the studies were classified into two categories: (a) sugarcane silage without inoculant (untreated) and (b) sugarcane silage inoculated with L. plantarum (1 × 10⁵–1.8 × 10⁶ cfu/g of fresh forage) or with L. buchneri (2.5 × 10⁴–1 × 10⁶ cfu/g of fresh forage; inoculated). Sugarcane silage inoculated with L. plantarum displayed increased ethanol concentration (+43.8%, p
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effects of propionic acid and Lactobacillus buchneri ufla sil 72 addition on fermentative and microbiological characteristics of sugar cane silage treated with and without calcium oxide
Grass and Forage Science, 2012Co-Authors: B F Carvalho, C.l.s. Ávila, Jose Cardoso Pinto, M N Pereira, Rosane Freitas SchwanAbstract:The objective of this work was to evaluate the effects of a new strain of Lactobacillus buchneri (UFLA SIL 72) isolated from sugar cane (Saccharum spp.) silage and the addition of propionic acid [1% based on fresh matter (FM)] to silages treated with and without calcium oxide (1% of FM) at 60 and 170 d of ensiling. A randomized block design with a 2 × 2 × 2 × 2 factorial arrangement of treatments was used to analyse the results. The use of calcium oxide reduced the ethanol content and neutral detergent fibre in all silages, increased pH values and favoured the growth of clostridia and yeasts. The addition of propionic acid reduced the yeast population, but it was not able to reduce ethanol content of silage. The addition of L. buchneri resulted in silages with higher concentration of propionate, reduced the levels of ethanol and reduced the population of clostridia in all silages. The use of calcium oxide is not recommended for silage of sugar cane.
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effects of an indigenous and a commercial Lactobacillus buchneri strain on quality of sugar cane silage
Grass and Forage Science, 2009Co-Authors: C.l.s. Ávila, Jose Cardoso Pinto, Henrique Cesar Pereira Figueiredo, Rosane Freitas SchwanAbstract:The aim was to evaluate the effects of adding a novel Lactobacillus buchneri strain and a commercial inoculant on the fermentation and aerobic stability of sugar cane silages (Saccharum spp.). In the first experiment samples were collected from sugar cane silage at 5, 20, 40 and 80 d after ensilage in experimental silos and microorganisms belonging to the Lactobacillus genus were isolated and identified, with a wild strain of L. buchneri, UFLA SIL 72, being selected as an inoculant. In the second experiment sugar cane was inoculated with either the novel bacteria or a commercial inoculant at the moment of ensiling and compared with a control silage prepared without an inoculant. Experimental silos were opened at 0, 3, 10, 30, 60 and 90 d of ensilage and their chemical composition measured. The silages opened after 90 d were also assessed for aerobic stability. The addition of L. buchneri resulted in a higher concentration of acetic acid and reduced populations of yeasts in silage compared to the other silage treatments, and a lower ethanol concentration in the silage. The novel L. buchneri isolate and the commercial inoculant also improved aerobic stability of the sugar cane silages. It was concluded that the addition of the novel inoculants L. buchneri UFLA SIL 72 to sugar cane silage can be recommended.
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estabilidade aerobia de silagens de capim mombaca tratadas com Lactobacillus buchneri
Revista Brasileira De Zootecnia, 2009Co-Authors: C.l.s. Ávila, Odilon Gomes Pereira, Jose Cardoso Pinto, Henrique Cesar Pereira Figueiredo, Augusto Ramalho De Morais, Rosane Freitas SchwanAbstract:Objetivou-se avaliar o efeito da adicao de duas cepas de Lactobacillus buchneri sobre a estabilidade aerobia de silagens de capim-mombaca. O experimento foi conduzido em delineamento inteiramente casualizado com tres repeticoes, em esquema de parcelas subdivididas no tempo, de modo que os tratamentos (silagens sem inoculantes e com inoculante experimental e comercial) foram aleatorizados nas parcelas e os tempos de avaliacao aerobia das silagens (0, 2, 8 ou 10 dias apos a abertura dos silos) nas subparcelas. Apos 90 dias de armazenamento, os silos foram abertos e amostras de aproximadamente 2,5 kg foram retiradas para avaliacao da estabilidade aerobia com base na temperatura de cada amostra. As mudancas quimicas e microbiologicas foram avaliadas. Apos abertura dos silos, verificou-se aumento nas populacoes de bacterias do acido latico, leveduras e fungos filamentosos. Houve aumento nos valores de pH resultante da reducao da concentracao dos acidos orgnicos e de aumentos dos teores de materia seca e proteina bruta. A adicao de L. buchneri as silagens melhorou sua estabilidade, enquanto a silagem sem inoculante apresentou estabilidade de 55 horas. As silagens inoculadas nao ultrapassaram essa temperatura limite em um periodo de 10 dias. A melhoria da estabilidade foi ocasionada pela associacao de altas concentracoes de cido acetico ao acido propionico e pela menor concentracao de acido latico no momento de abertura, o que inibiu a proliferacao de fungos filamentosos e leveduras apos abertura dos silos.
Steve L Taylor - One of the best experts on this subject based on the ideXlab platform.
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factors controlling histamine production in swiss cheese inoculated with Lactobacillus buchneri
Journal of Dairy Science, 1990Co-Authors: S S Sumner, Steve L Taylor, Frances RocheAbstract:Abstract Swiss cheese was made from raw milk inoculated with various concentrations of a histamine-producing strain of Lactobacillus buchneri . Histamine production in these cheeses was proportional to the initial number of L. buchneri present in the raw milk. The highest inoculum level tested was 10 5 L. buchneri /ml. This cheese contained 80mg of histamine/100g of cheese after 90 d of storage. Only 15mg of histamine/100g of cheese were detected after 90 d at the lowest inoculum level, 10 2 L. buchneri /ml. No histamine was detected in any of the Swiss cheese samples until after the brining stage. Perceptible growth of L. buchneri also did not occur until after the warm room treatment. Therefore, control of histamine formation in Swiss cheese requires control of the number of histamine-producing bacteria in the raw milk. A 5.5% NaCl concentration in DeMan, Rogosa, Sharpe (MRS) broth inhibited the production of histamine by L. buchneri , but the concentrations of NaCl typically found in Swiss cheese were not inhibitory. The histamine-producing isolate of L. buchneri survived heating at 49 to 80°C for 10min, suggesting that this organism would easily survive the normal heating process applied to raw milk used prior to making Swiss cheese.
Ricardo Andrade Reis - One of the best experts on this subject based on the ideXlab platform.
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Meta‐analysis of the effects of Lactobacillus plantarum and Lactobacillus buchneri on fermentation, chemical composition and aerobic stability of sugarcane silage
Grassland Science, 2018Co-Authors: Carlos Henrique Silveira Rabelo, Carla Joice Härter, C.l.s. Ávila, Ricardo Andrade ReisAbstract:We conducted a meta‐analysis to investigate the impact of Lactobacillus plantarum or Lactobacillus buchneri on the fermentation, chemical composition, and aerobic stability of sugarcane silage. Two databases that included 15 and 27 published articles (81 and 83 silages evaluated) were used to evaluate L. plantarum and L. buchneri, respectively. For each database, the means of the treatments compiled from the studies were classified into two categories: (a) sugarcane silage without inoculant (untreated) and (b) sugarcane silage inoculated with L. plantarum (1 × 10⁵–1.8 × 10⁶ cfu/g of fresh forage) or with L. buchneri (2.5 × 10⁴–1 × 10⁶ cfu/g of fresh forage; inoculated). Sugarcane silage inoculated with L. plantarum displayed increased ethanol concentration (+43.8%, p
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effects of Lactobacillus buchneri as a silage inoculant and as a probiotic on feed intake apparent digestibility and ruminal fermentation and microbiology in wethers fed low dry matter whole crop maize silage
Grass and Forage Science, 2018Co-Authors: Carlos Henrique Silveira Rabelo, Fernanda Carvalho Basso, E. C. Lara, Carla Joice Härter, L G O Jorge, L G Mesquita, Luis Felipe Prada E Silva, Ricardo Andrade ReisAbstract:Lactobacillus buchneri was investigated as a silage inoculant and as a probiotic on feed intake, apparent digestibility, and ruminal fermentation and microbiology in wethers fed low-dry-matter (DM) whole-crop maize silage. Maize forage (279 g/kg DM) was ensiled without inoculant (untreated) and with L. buchneri CNCM I-4323 at 1 × 105 cfu/g fresh forage (inoculated). Six cannulated wethers were arranged in a double 3 × 3 Latin square and assigned to one of three diets: (i) untreated maize silage (untreated), (ii) inoculated maize silage (inoculated), and (iii) untreated maize silage with a daily dose of L. buchneri (1 × 107 cfu/g supplied silage) injected directly into the rumen (LB-probiotic). Wethers fed the inoculated diet had a higher (p = .050) DM intake (1.30% body weight [BW]) than wethers fed untreated and LB-probiotic diets (1.17% and 1.18% BW respectively). The relative proportion of Ruminococcus flavefaciens (proportion of total estimated rumen bacterial 16S rDNA) in the rumen of wethers fed inoculated and LB-probiotic diets (both 0.42%) tended (p = .098) to be lower than in the untreated diet (0.83%). Lactobacillus buchneri as a silage inoculant or as a probiotic had little effect on the variables measured in wethers.
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Influence of Lactobacillus buchneri as silage additive and forage: concentrate ratio on the growth performance, fatty acid profile in longissimus muscle, and meat quality of beef cattle
Canadian Journal of Animal Science, 2016Co-Authors: Carlos Henrique Silveira Rabelo, Fernanda Carvalho Basso, Telma Teresinha Berchielli, Tim A. Mcallister, Josiane Fonseca Lage, Gustavo José Correa Gonçalves, E. C. Lara, A. D. Oliveira, Ricardo Andrade ReisAbstract:Our objective was to investigate Lactobacillus buchneri as a silage additive and the forage:concentrate (F:C) ratio on growth performance and meat quality of finishing beef cattle. The trial was a 2 (corn silage untreated or inoculated with L. buchneri) × 2 (two F:C ratios, 60:40 or 40:60) factorial. Bulls fed a 40:60 F:C diet containing inoculated silage had greater dry matter (DM) intake (P
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influence of Lactobacillus buchneri as silage additive and forage concentrate ratio on the growth performance fatty acid profile in longissimus muscle and meat quality of beef cattle
Canadian Journal of Animal Science, 2016Co-Authors: Carlos Henrique Silveira Rabelo, Fernanda Carvalho Basso, Telma Teresinha Berchielli, Tim A. Mcallister, Josiane Fonseca Lage, Gustavo José Correa Gonçalves, E. C. Lara, A. D. Oliveira, Ricardo Andrade ReisAbstract:Our objective was to investigate Lactobacillus buchneri as a silage additive and the forage:concentrate (F:C) ratio on growth performance and meat quality of finishing beef cattle. The trial was a 2 (corn silage untreated or inoculated with L. buchneri) × 2 (two F:C ratios, 60:40 or 40:60) factorial. Bulls fed a 40:60 F:C diet containing inoculated silage had greater dry matter (DM) intake (P < 0.01) and average daily gain (P = 0.029) compared with other treatments. DM (P = 0.02) and neutral detergent digestibility (P < 0.01) were depressed by inoculation of corn silage with L. buchneri. Thiobarbituric acid concentration in meat increased (P < 0.01) by 15% in bulls fed the 60:40 F:C diet compared with the 40:60 F:C diet. Inoculation of silage increased (P = 0.02) the ratio of unsaturated fatty acids to saturated fatty acids. Compared with the 60:40 F:C diet, the concentrations of polyunsaturated fatty acids (P = 0.03) and omega-6 fatty acids (P = 0.02) increased in longissimus muscle by 23% and 26%, respe...
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fermentation and aerobic stability of high moisture corn silages inoculated with different levels of Lactobacillus buchneri
Revista Brasileira De Zootecnia, 2012Co-Authors: Fernanda Carvalho Basso, Carlos Henrique Silveira Rabelo, T F Bernardes, Anna Paula De Toledo Piza Roth, Ana Claudia Ruggieri, Ricardo Andrade ReisAbstract:Fermentation and aerobic stability were evaluated in high-moisture corn (HMC) silage inoculated with different levels of Lactobacillus buchneri. The HMC composed of 654 g/kg dry matter (DM) was ensiled in quadruplicate laboratory silos (7 L) per treatment. L. buchneri 40788 was applied at 5 × 104; 1 × 105; 5 × 105; and 1 × 106 cfu/g to the ground corn. Silages with no additive were used as controls. After 140 d of ensiling, the silages were subjected to an aerobic stability evaluation for 12 days in which the chemical parameters, microbiological parameters and silage temperature were measured to determine the aerobic deterioration. The lactic acid, acetic acid and propionic acid concentrations did not differ between silages. The fermentation parameters of HMC were not affected by L. buchneri. The HMC containing L. buchneri had a low number of yeast and mould colonies and a more stable pH until in the eighth measurement, which improved the aerobic stability without affecting gas loss. Doses of L. buchneri greater than or equal to 5 × 105 cfu/g applied to the HMC were the most efficient in control of aerobic deterioration.
