Longidorus

14,000,000 Leading Edge Experts on the ideXlab platform

Scan Science and Technology

Contact Leading Edge Experts & Companies

Scan Science and Technology

Contact Leading Edge Experts & Companies

The Experts below are selected from a list of 312 Experts worldwide ranked by ideXlab platform

D J F Brown - One of the best experts on this subject based on the ideXlab platform.

  • description of Longidorus cholevae sp n nematoda dorylaimida from a riparian habitat in the rila mountains bulgaria
    ZooKeys, 2013
    Co-Authors: Vlada Peneva, Stela Lazarova, Francesca De Luca, D J F Brown
    Abstract:

    A description is provided of Longidorus cholevae sp. n., a bisexual species associated with wild cherry (Prunus avium L.) from the Rila Mountains, Bulgaria. The position of L. cholevae sp. n. among other species of the genus was elucidated by using morphological and molecular data. Phylogenetic analyses were performed of D2-D3 expansion domains of the 28S rRNA and the partial ITS1 containing regions by Neighbor-Joining, Maximum Likelihood and Bayesian Inference methods. The species is characterised by a female body length of 6.1-8.1 mm; long odontostyle (106-129 μm); lip region wide (21.5-24 μm) rounded and continuous with the body profile; amphidial pouches short and wide, funnel-shaped; a posteriorly situated guide ring (30-37 μm); normal arrangement of pharyngeal glands, and short bluntly rounded to hemispherical tail. Four juvenile stages indentified, first stage with elongate conoid tail. Males with 2-4 adanal pairs and a row of 11-13 single ventromedian supplements, spicules 96-120 μm long. Based both on morphological and molecular data the new species appearred to be the most similar witha group of species distributed in Europe sharing common charcters such as amphidial fovea, lip region and tail shapes, and having similar odontostyle and body length: L. poessneckensis, L. caespiticola, L. macrososma, L. helveticus, L. carniolensis and L. pius. An updated list of Longidorus species and a partial polytomous keys to the Longidorus species with long odontostyle (code A45) and short tail (code H1) are provided.

  • Longidoridae and nepoviruses in Bulgaria and Slovenia
    Helminthologia, 2012
    Co-Authors: V. K. Peneva, M. Knapič, Simon Širca, Simona Lazarova, Milka Elshishka, Gregor Urek, D J F Brown
    Abstract:

    Data on the distribution of Longidoridae and nepoviruses in Bulgaria and Slovenia are summarized. Six species of Longidorus (L. apulus, L. attenuatus, L. arthensis, L. fasciatus, L. elongatus, L. macrosoma), one ParaLongidorus species (P. maximus) and three Xiphinema species (X. diversicaudatum, X. index, X. rivesi) are known as natural vectors of nine nepoviruses in Europe. Currently, 10 and 13 species of Xiphinema; 6 and 15 of Longidorus are reported to occur in Slovenia and Bulgaria, respectively. ParaLongidorus maximus has been reported only in Bulgaria. Among the virus vector species X. index, X. diversicaudatum and L. elongatus occur in both countries, X. rivesi only in Slovenia and L. attenuatus, L. macrosoma, X. italiae and P. maximus only in Bulgaria. A report of X. index and Grapevine fanleaf virus (GFLV) in Bulgaria was related to transgenic grape tolerance to the same virus. Nepoviruses have been reported from Slovenia, but despite an evident relationship in the occurrence of GFLV and X. index in several vineyards the only laboratory proven transmission is that of TRSV and ToRSV to bait plants by a Slovenian population of X. rivesi.

  • description of Longidorus hangzhouensis sp n nemata longidoridae from zhejiang province new geographical records of l henanus xu cheng 1992 and an identification key for Longidorus species occurring in china
    Nematology, 2001
    Co-Authors: Jingwu Zheng, Robert T Robbins, Deliang Peng, D J F Brown
    Abstract:

    Specimens of a Longidorus species, here described as L. hangzhouensis sp. n., were recovered from the rhizosphere of sweet-scented Osmanthus growing in Hangzhou, Zhejiang province, China, and a second population was collected from Camellia japonica, Fuyang, Zhejiang province. The species is characterised by medium-sized body length (3.1-4.9 mm), head region narrow (8-12 μm), anteriorly rounded, with continuous, funnel-shaped amphidial pouches, a relatively long odontostyle (97-126 μm) and a short, almost hemispherical tail. Three juvenile development stages were identified, J2, J3 and J4. A second Longidorus species, identified as L. henanus Xu & Cheng, 1990, previously known only from the type locality in Henan province, was recovered from the rhizosphere of bamboo from Beijing and of poplar from Yuanqu county, Shanxi province. A dichotomous key is provided for the Longidorus species reported from China.

  • Longidorus camelliae n sp nematoda longidoridae associated with ornamental cultivars of camellia japonica l growing in a nursery at fuyang zhejiang province eastern china
    Systematic Parasitology, 2000
    Co-Authors: Jingwu Zheng, V Peneva, D J F Brown
    Abstract:

    A description is provided of Longidorus camelliae n. sp., a bisexual species associated with ornamental cultivars of Camellia japonica L. growing in Fuyang, Zhejiang Province, eastern China. The species is characterised by its small body length (1.83–3.54 mm), narrow (6–10 μm), anteriorly flattened, continuous head region, non-bilobed amphidial pouches, medium odontostyle length (80–91 μm) and elongate, conoid tail (32–39 μm; c′=1.1–1.4). Males are characterised by having short spicules (44–48 μm) and a row of seven supplements. The species has only three juvenile development stages.

  • Longidorus dalmassoi sp n nematoda dorylaimoidea from france
    Nematology, 1999
    Co-Authors: Vlada Peneva, Pieter A A Loof, D J F Brown
    Abstract:

    A description is provided of Longidorus dalmassoi sp. n., a bisexual species associated with grasses in France. The species is characterised by a body length of 6.9-7.8 mm, slightly expanded and anteriorly flattened head region, symmetrically bilobed amphidial pouches, long odontostyle (152-163 mu m), spear guiding system with compensation sacks, conoid tail (39-52.5 mu m) with narrow, rounded terminus, males with short spicules (50-51 mu m) and a row of 15 or 16 supplements. Longidorus dalmassoi sp. n. (Nematoda: Longidoridae) provenant de France - Description est donnee de Longidorus dalmassoi sp. n., espece bisexuee associee a une vegetation herbacee, en France. Cette espece est caracterisee par une longueur du corps de 6,9-7,8 mm, une extremite anterieure legerement elargie et aplatie a l'avant, des poches amphidiennes symetriques et bilobees, un odontostyle long (152-163 mu m), un systeme de guidage du stylet comportant des sacs de compensation, une queue conoide (39-52,5 mu m) a extremite etroite et arrondie, des males a spicules courts (20-51 mu m) et pourvus d'une rangee de 15 ou 16 supplements.

Pablo Castillo - One of the best experts on this subject based on the ideXlab platform.

  • an integrative taxonomic study of the needle nematode complex Longidorus goodeyi hooper 1961 nematoda longidoridae with description of a new species
    European Journal of Plant Pathology, 2020
    Co-Authors: Carolina Cantalapiedranavarrete, Pablo Castillo, Juan E Palomaresrius, Ruihang Cai, Thomas Prior, Bex Lawson, Antonio Archidonayuste
    Abstract:

    Needle nematodes are polyphagous root-ectoparasites parasitizing a wide range of economically important plants not only by directly feeding on root cells, but also by transmitting nepoviruses. This study deciphers the diversity of the complex Longidorus goodeyi through integrative diagnosis method, based on a combination of morphological, morphometrical, multivariate analysis and molecular data. A new Longidorus species, Longidorus panderaltum n. sp. is described and illustrated from a population associated with the rhizosphere of asphodel (Asphodelus ramosus L.) in southern Spain. Morphologically, L. panderaltum n. sp. is characterized by having a moderately long female body (5.2-7.0 mm), lip region bluntly rounded and slightly offset by a depression with body contour, amphidial pouch with slightly asymmetrical lobes, odontostyle 80.5–101.0 µm long, tail short and conoid rounded. Longidorus panderaltum n. sp. is quite similar to L. goodeyi and L. onubensis in major morphometrics and morphology. However, differential morphology in the tail shape of first-stage juvenile, phylogeny and haplonet analyses indicate they are three distinct valid species. This study defines those three species as members of L. goodeyi complex group and reveals the taxonomical complexity of the genus Longidorus. This L. goodeyi complex group demonstrated that the biodiversity of Longidorus in this region is still not fully clarified.

  • integrative descriptions and molecular phylogeny of two new needle nematodes of the genus Longidorus nematoda longidoridae from spain
    European Journal of Plant Pathology, 2020
    Co-Authors: Antonio Archidonayuste, Carolina Cantalapiedranavarrete, Juan E Palomaresrius, Ruihang Cai, Pablo Castillo
    Abstract:

    Needle nematodes have an economic importance by causing damage to a wide range of natural and cultivated plants not only by directly feeding on root cells, but also by transmitting plant nepoviruses. Two new Longidorus nematodes, Longidorus oakcrassus n. sp. and Longidorus oakgracilis n. sp., are described and illustrated from populations associated with the rhizosphere of Pyrenean oak (Quercus pyrenaica Wild.) in southern Spain. The taxonomic position of both new species within the genus was assigned using an integrative approach. Morphologically, L. oakcrassus n. sp. is characterized by a female with a large and robust body size (9.2–12.2 mm), lip region anteriorly flattened to slightly rounded and almost continuous or slightly offset by a depression with body contour, ca 25.5–32.0 μm wide, amphidial fovea with slightly asymmetrical lobes, stylet composed by an odontostyle moderately long (110.0–133.5 μm) and odontophore weakly developed, pharynx short ending in a terminal pharyngeal bulb with common arrangement of pharyngeal glands, tail short almost hemispherical shape. Longidorus oakgracilis n. sp. is characterized by having a moderately long and thin female body (5.4–7.9 mm in length), a bluntly-rounded lip region, set off from body contour by a slight depression, amphidial fovea funnel-shaped without lobe, odontostyle moderately long (94.0–106.0 μm), pharyngeal bulb with common arrangement of pharyngeal glands, short tail, bluntly hemispherical. The presence of males is common in both species. Integrative diagnosis was based on molecular data using D2-D3 expansion domains of the 28S rRNA, 18S rRNA, ITS1 rRNA and partial coxI gene sequences and morphology. Although different gene markers show variations in the phylogenetic relationships, phylogeny indicated that L. oakcrassus n. sp. is phylogenetically related with several species described from the Iberian Peninsula, including L. oakgracilis n. sp., which is clustered with L.cf. olegi, L. lusitanicus and L. silvestris.

  • first reports morphological and molecular characterization of Longidorus caespiticola and Longidorus poessneckensis nematoda longidoridae from ukraine
    Journal of Nematology, 2018
    Co-Authors: Solomia Susulovska, Pablo Castillo, Antonio Archidonayuste
    Abstract:

    Seven needle nematode species of the genus Longidorus have been reported in Ukraine. Nematological surveys for needle nematodes were carried out in Ukraine between 2016 and 2017 and two nematode species of Longidorus ( L. caespiticola and L. poessneckensis ) were collected from natural and anthropogenically altered habitats on the territory of Opillia and Zakarpattia in Ukraine. Nematodes were extracted from 500 cm 3 of soil by modified sieving and decanting method. Extracted specimens were processed to glycerol and mounted on permanent slides and subsequently identified morphologically and molecularly. Nematode DNA was extracted from single individuals and PCR assays were conducted as previously described for D2–D3 expansion segments of 28S rRNA. Sequence alignments for D2–D3 from L. caespiticola showed 97%–99% similarity to other sequences of L. caespiticola deposited in GenBank from Belgium, Bulgaria, Czech Republic, Russia, Slovenia, and Scotland. Similarly, D2–D3 sequence align- ments from L. poessneckensis , showed 99% to other sequences of L. poessneckensis deposited in GenBank from Slovakia and Czech Republic. Morphology, morphometry, and molecular data obtained from these samples were consistent with L. caespiticola and L. poessneckensis identification. To our knowledge, these are the first reports of L. caespiticola and L. poessneckensis in Ukraine, extending the geographical distribution of these species.

  • morphological and molecular identification of Longidorus euonymus and helicotylenchus multicinctus from the rhizosphere of grapevine and banana in greece
    Journal of Nematology, 2017
    Co-Authors: Emmanuel A. Tzortzakakis, Carolina Cantalapiedranavarrete, Pablo Castillo, Juan E Palomaresrius, Antonio Archidonayuste
    Abstract:

    Plant-parasitic nematodes such as Longidorus euonymus and Helicotylenchus multicintctus are species widely distributed in central Europe as well as in Mediterranean area. In Greece, both species have been previously reported but no morphometrics or molecular data were available for these species. Nematode surveys in the rhizosphere of grapevines in Athens carried out in 2016 and 2017, yielded a Longidorus species identified as Longidorus euonymus. Similarly, a population of Helicotylenchus multicinctus was detected infecting banana roots from an outdoor crop in Tertsa, Crete. For both species, morphometrics and molecular data of Greek populations were provided, resulting in the first integrative identification of both nematode species based on morphometric and molecular markers, confirming the occurrence of these two nematodes in Greece as had been stated in earlier reports.

  • Molecular characterization and distribution of the needle nematode Longidorus laevicapitatus Williams, 1959 (Nematoda: Longidoridae) in Costa Rica
    European Journal of Plant Pathology, 2017
    Co-Authors: Walter Peraza-padilla, Antonio Archidona-yuste, Carolina Cantalapiedra-navarrete, Juan E. Palomares-rius, Tatiana Zamora-araya, Pablo Castillo
    Abstract:

    Correct identification of Longidorus species in Costa Rica is essential to establish appropriate control strategies for preventing the spread of these nematodes. Nematode surveys conducted in the rainy seasons from 2013 to 2015 in areas arbitrarily chosen and widely distributed in the whole territory of Costa Rica, resulted in an overall prevalence of Longidorus spp. infesting soils both cultivated, ornamental and wild plants of 40.26 %. Integrative morphometric and molecular data for Longidorus populations were obtained using D2-D3 expansion segments of 28S rRNA, ITS1-rRNA, and the partial 18S-rRNA, identifying a solely species identified as Longidorus laevicapitatus . Morphology and morphometrical traits analysis of these populations of L. laevicapitatus were in agreement with those of the original and posterior descriptions of the species, except for some minor differences, which may be a result of intraspecific variability. The phylogenetic relationships of this species with other representatives of Longidorus spp. using D2-D3 expansion segments and the partial 18S indicated that L. laevicapitatus clustered clearly separately in a basal position in both phylogenetic trees.

Castillo Pablo - One of the best experts on this subject based on the ideXlab platform.

  • An integrative taxonomic study of the needle nematode complex Longidorus goodeyi Hooper, 1961 (Nematoda: Longidoridae) with description of a new species
    'Springer Science and Business Media LLC', 2021
    Co-Authors: Cai Ruihang, Cantalapiedra-navarrete C., Castillo Pablo, Palomares Rius, Juan E., Prior Tom, Lawson Bex, Archidona-yuste Antonio
    Abstract:

    Needle nematodes are polyphagous root-ectoparasites parasitizing a wide range of economically important plants not only by directly feeding on root cells, but also by transmitting nepoviruses. This study deciphers the diversity of the complex Longidorus goodeyi through integrative diagnosis method, based on a combination of morphological, morphometrical, multivariate analysis and molecular data. A new Longidorus species, Longidorus panderaltum n. sp. is described and illustrated from a population associated with the rhizosphere of asphodel (Asphodelus ramosus L.) in southern Spain. Morphologically, L. panderaltum n. sp. is characterized by having a moderately long female body (5.2-7.0 mm), lip region bluntly rounded and slightly offset by a depression with body contour, amphidial pouch with slightly asymmetrical lobes, odontostyle 80.5–101.0 µm long, tail short and conoid rounded. Longidorus panderaltum n. sp. is quite similar to L. goodeyi and L. onubensis in major morphometrics and morphology. However, differential morphology in the tail shape of first-stage juvenile, phylogeny and haplonet analyses indicate they are three distinct valid species. This study defines those three species as members of L. goodeyi complex group and reveals the taxonomical complexity of the genus Longidorus. This L. goodeyi complex group demonstrated that the biodiversity of Longidorus in this region is still not fully clarified.This research was financially supported by grant 201740E042, “Análisis de diversidad molecular, barcoding, y relaciones filogenéticas de nematodos fitoparásitos en cultivos mediterráneos” from Spanish National Research Council (CSIC), and by the Humboldt Research Fellowship for Postdoctoral Researchers awarded for the corresponding author. The first author acknowledges the China Scholarship Council (CSC) for financial support. The fifth author acknowledges Spanish Ministry of Economy and Competitiveness for the “Ramon y Cajal” Fellowship RYC-2017-22228. The corresponding author is a recipient of Humboldt Research Fellowship for Postdoctoral Researchers at Helmholtz Centre for Environmental Research-UFZ, Leipzig, Germany. Open Access funding provided by Projekt DEAL

  • Integrative descriptions and molecular phylogeny of two new needle nematodes of the genus Longidorus (Nematoda: Longidoridae) from Spain
    'Springer Science and Business Media LLC', 2021
    Co-Authors: Cai Ruihang, Cantalapiedra-navarrete C., Palomares Rius, Juan E., Archidona-yuste Antonio, Castillo Pablo
    Abstract:

    Needle nematodes have an economic importance by causing damage to a wide range of natural and cultivated plants not only by directly feeding on root cells, but also by transmitting plant nepoviruses. Two new Longidorus nematodes, Longidorus oakcrassus n. sp. and Longidorus oakgracilis n. sp., are described and illustrated from populations associated with the rhizosphere of Pyrenean oak (Quercus pyrenaica Wild.) in southern Spain. The taxonomic position of both new species within the genus was assigned using an integrative approach. Morphologically, L. oakcrassus n. sp. is characterized by a female with a large and robust body size (9.2–12.2 mm), lip region anteriorly flattened to slightly rounded and almost continuous or slightly offset by a depression with body contour, ca 25.5–32.0 μm wide, amphidial fovea with slightly asymmetrical lobes, stylet composed by an odontostyle moderately long (110.0–133.5 μm) and odontophore weakly developed, pharynx short ending in a terminal pharyngeal bulb with common arrangement of pharyngeal glands, tail short almost hemispherical shape. Longidorus oakgracilis n. sp. is characterized by having a moderately long and thin female body (5.4–7.9 mm in length), a bluntly-rounded lip region, set off from body contour by a slight depression, amphidial fovea funnel-shaped without lobe, odontostyle moderately long (94.0–106.0 μm), pharyngeal bulb with common arrangement of pharyngeal glands, short tail, bluntly hemispherical. The presence of males is common in both species. Integrative diagnosis was based on molecular data using D2-D3 expansion domains of the 28S rRNA, 18S rRNA, ITS1 rRNA and partial coxI gene sequences and morphology. Although different gene markers show variations in the phylogenetic relationships, phylogeny indicated that L. oakcrassus n. sp. is phylogenetically related with several species described from the Iberian Peninsula, including L. oakgracilis n. sp., which is clustered with L.cf. olegi, L. lusitanicus and L. silvestris.This research was financially supported by grant 201740E042, “Análisis de diversidad molecular, barcoding, y relaciones filogenéticas de nematodos fitoparásitos en cultivos mediterráneos” from Spanish National Research Council (CSIC). Authors thank G. León Ropero and J. Martín Barbarroja (IAS-CSIC) for the excellent technical assistance. The first author acknowledges the China Scholarship Council (CSC) for financial support. The second author is a recipient of Humboldt Research Fellowship for Postdoctoral Researchers at Helmholtz Centre for Environmental Research-UFZ, Leipzig, Germany. The fourth author acknowledges Spanish Ministry of Economy and Competitiveness for the “Ramon y Cajal” Fellowship RYC-2017-22228

  • Molecular phylogenetic analysis and comparative morphology reveals the diversity and distribution of needle nematodes of the genus Longidorus (Dorylaimida: Longidoridae) from Spain
    'Brill', 2019
    Co-Authors: Archidona-yuste Antonio, Cantalapiedra-navarrete C., Castillo Pablo, Palomares Rius, Juan E.
    Abstract:

    The genus Longidorus constitutes a large group of approximately 170 species of plant-ectoparasitic nematodes that are polyphagous and distributed almost worldwide. Some of the species of this genus are vectors of plant viruses. Species discrimination in Longidorus is difficult because the morphology is very conservative, and morphometric characters often overlap, leading to potential misidentification. Integrative taxonomy, based on the combination of molecular analyses with morphology, is a useful and necessary approach in Longidorus species identification. In Spain from 2014 to 2017, we conducted nematode surveys among cultivated and wild plants, from which we identified 13 populations of Longidorus, two of which appeared to represent new species and are described herein as L. iliturgiensis sp. nov. and L. pacensis sp. nov., and 11 populations belonging to eight known species: L. africanus, L. baeticus, L. carpetanensis, L. fasciatus, L. nevesi, L. cf. olegi, L. pini, and L. vallensis. Three species are new geographical records for Spain (L. nevesi, L. cf. olegi, and L. africanus). We report molecular data for L. nevesi, L. cf. olegi, L. carpetanensis and L. pini for the first time. Additionally, we describe the males of L. pini and the juveniles of L. cf. olegi.Our research was supported by grants P12-AGR-1486 from ‘Consejería de Economía, Innovación y Ciencia’ of Junta de Andalucía, 219262 ArimNET_ERANET FP7 2012–2015 Project PESTOLIVE ‘Contribution of olive history for the management of soilborne parasites in the Mediterranean basin’ from Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), and FEDER financial support from the European Union is gratefully acknowledged. Antonio J. Archidona-Yuste is a recipient of the research fellowship BES-2013-063495 from the Spanish ‘Ministerio de Economía y Competitividad’.Peer reviewe

  • Morphological and Molecular Characterization of ParaLongidorus sali Siddiqi, Hooper, and Khan, 1963 with a Description of the First-Stage Juvenile and Male of Longidorus jonesi Siddiqi, 1962 from China
    'Exeley Inc.', 2018
    Co-Authors: Cai Ruihang, Castillo Pablo, Maria Munawar, Qu Nan, Zheng Jing-wu
    Abstract:

    The family Longidoridae Thorne, 1935 comprises a group of migratory plant-parasitic species that damage a wide range of wild and cultivated plants through direct feeding on root cells and the transmission of several plant-pathogenic viruses (Decraemer and Robbins, 2007; Decraemer and Chaves, 2012). Members of these genera are known to transmit nepoviruses and are regulated by quarantine inspections in many countries (Gutiérrez-Gutiérrez et al., 2016). Longidorus and ParaLongidorus belong to family Longidoridae, both are globally distributed and have 160 and 90 known species, respectively (Palomares-Rius et al., 2013; Archidona-Yuste et al., 2016; Esmaeili et al., 2016). Some species of ParaLongidorus have controversial status due to synonymization of Longidoroides (Khan et al., 1978) and Siddiqia (Khan et al., 1978), and some ParaLongidorus species have been wrongly included and belong to genus Longidorus (Decraemer and Coomans, 2007). The major difference used to separate Longidorus, Longidoroides, and ParaLongidorus is the shape of amphids (pouch like in Longidorus and Longidoroides vs. funnel/stirrup shaped in ParaLongidorus) and the opening of amphidial aperture (pore-like in Longidorus vs. slit-like in Longidoroides and ParaLongidorus) (Oliveira and Neilson, 2006). Several new species of ParaLongidorus have published with complete molecular characterization and scanning electron microscopy (SEM) observations (Palomares-Rius et al., 2008, 2013; Pedram et al., 2012; Gutiérrez-Gutiérrez et al., 2017; Barsi and Luca, 2017) which enables the discrimination between Longidorus and ParaLongidorus species. However, there is no molecular evidence to distinguish Longidoroides species which leaves the status of this genus as junior synonym of ParaLongidorus as suggested by Decraemer and Coomans (2007). During a routine nematological survey of Hangzhou, Zhejiang Province, eastern China, two populations of longidorid nematodes were isolated from the rhizosphere of woody perennials. The population isolated from Cyclobalanopsis glauca (Thumb.) Oerst, 4 juvenile stages were recovered and identified as Longidorus jonesi, the population from Castanopsis sclerophylla (Lindl.) Schottky, 3 juvenile stages were recovered and was identified as ParaLongidorus sali. Robbins et al. (1995) reported 3 juveniles stages of L. jonesi and no first stage juvenile or male were observed additionally this is the first report of genus ParaLongidorus found in China. Therefore, the objectives of the present study were to: (i) provide updated morphological descriptions of first-stage juvenile P. sali, and male of L. jonesi, (ii) characterize the molecular data of both species using the D2–D3 expansion segments of 28S rRNA and partial 18S rRNA gene sequences, and (iii) demonstrate the phylogenetic relationships of both species with related species.This research was supported by the National Natural Science Foundation of China (No: 31772137).Peer reviewe

  • Morphological and Molecular Identification of Longidorus euonymus and Helicotylenchus multicinctus from the Rhizosphere of Grapevine and Banana in Greece
    'Exeley Inc.', 2017
    Co-Authors: Tzortzakakis E. A., Cantalapiedra-navarrete C., Castillo Pablo, Palomares Rius, Juan E., Archidona-yuste Antonio
    Abstract:

    Plant-parasitic nematodes such as Longidorus euonymus and Helicotylenchus multicintctus are species widely distributed in central Europe as well as in Mediterranean area. In Greece, both species have been previously reported but no morphometrics or molecular data were available for these species. Nematode surveys in the rhizosphere of grapevines in Athens carried out in 2016 and 2017, yielded a Longidorus species identified as Longidorus euonymus. Similarly, a population of Helicotylenchus multicinctus was detected infecting banana roots from an outdoor crop in Tertsa, Crete. For both species, morphometrics and molecular data of Greek populations were provided, resulting in the first integrative identification of both nematode species based on morphometric and molecular markers, confirming the occurrence of these two nematodes in Greece as had been stated in earlier reports.Peer reviewe

R. T. Robbins - One of the best experts on this subject based on the ideXlab platform.

  • Longidorus ferrisi n sp from california citrus
    Journal of Nematology, 2009
    Co-Authors: R. T. Robbins, Majid Pedram
    Abstract:

    In October 1999, the authors received fixed specimens of a species of Longidorus from Howard Ferris found about the roots of a citrus tree in Oakville, Napa County, CA. After determining it to be new a species, we requested additional specimens. The samples contained roughly equal numbers of males and females. Longidorus ferrisi n. sp. is most similar to L. elongatus, but can be distinguished by a greater c-ratio (111-187 vs 73-141), a lesser c (0.7-1.1 vs 1.0-1.3), a more offset head, a more posterior guide ring (35-40 vs 30-33 mm), the presence of sperm in the uterus in mature females, and the approximate 1:1 ratio of females to males. Other similar species include L. artemisiae, L. crassus, L. glycines, and L. milanis. Longidorus ferrisi n. sp. differs from L. artemisiae by a lesser a-ratio (74-102 vs 109-155), a lesser c value (0.7-1.1 vs 1.0-1.6), a more posterior guide ring (35-40 vs 27-34 mm), a longer odontostyle (91-108 vs 84-98 mm), a wider lip region (16-19 vs 14-17 mm), wider mid-body (53-69 vs 41-52 mm), and longer spicules (57-65 vs 39-49 mm). The new species differs substantially from L. crassus by its lip shape and the presence of males, and differs from L. glycines by a shorter body (4.33-5.97 vs 6.14-8.31 mm), a lesser c value (0.7-1.1 vs 0.9-1.4), a narrower lip region (16-19 vs 20-23 mm), wider mid-body (53-69 vs 39-57 mm), longer spicules (53-69 vs 45-53 mm), and fewer supplements (7-11 vs 11-17). Longidorus ferrisi n. sp. differs from L. milanis by a longer body (4.33-5.97vs 3.00-4.90 mm), a greater c value (111-187 vs 86-130), a wider mid-body (53-69 vs 43-56 mm), a different head shape, and longer spicules (53-69 vs 41-54 mm). The nuclear 18S ribosomal DNA sequence of this species revealed that this species is unique with respect to all sequenced Longidorus species.

  • morphological and molecular characterization of Longidorus americanum n sp nematoda longidoridae a needle nematode parasitizing pine in georgia
    Journal of Nematology, 2005
    Co-Authors: Z A Handoo, R. T. Robbins, S W Fraedrich, Sergei A. Subbotin, Lynn K Carta, Andrea M Skantar, M M Cram
    Abstract:

    We describe and illustrate a new needle nematode, Longidorus americanum n. sp., associated with patches of severely stunted and chlorotic loblolly pine, (Pinus taeda L.) seedlings in seedbeds at the Flint River Nursery (Byromville, GA). It is characterized by having females with a body length of 5.4-9.0 mm; lip region slightly swollen, anteriorly flattened, giving the anterior end a truncate appearance; long odontostyle (124-165 µm); vulva at 44%-52% of body length; and tail conoid, bluntly rounded to almost hemispherical. Males are rare but present, and in general shorter than females. The new species is morphologically similar to L. biformis, L. paravineacola, L. saginus, and L. tarjani but differs from these species either by the body, odontostyle and total stylet length, or by head and tail shape. Sequence data from the D2-D3 region of the 28S rDNA distinguishes this new species from other Longidorus species. Phylogenetic relationships of Longidorus americanum n. sp. with other longidorids based on analysis of this DNA fragment are presented. Additional information regarding the distribution of this species within the region is required.

  • cluster analysis of Longidorus species nematoda longidoridae a new approach in species identification
    Journal of Nematology, 2004
    Co-Authors: R. T. Robbins
    Abstract:

    Hierarchical cluster analysis based on female morphometric character means including body length, distance from vulva opening to anterior end, head width, odontostyle length, esophagus length, body width, tail length, and tail width were used to examine the morphometric relationships and create dendrograms for (i) 62 populations belonging to 9 Longidorus species from Arkansas, (ii) 137 published Longidorus species, and (iii) 137 published Longidorus species plus 86 populations of 16 Longidorus species from Arkansas and various other locations by using JMP 4.02 software (SAS Institute, Cary, NC). Cluster analysis dendograms visually illustrated the grouping and morphometric relationships of the species and populations. It provided a computerized statistical approach to assist by helping to identify and distinguish species, by indicating morphometric relationships among species, and by assisting with new species diagnosis. The preliminary species identification can be accomplished by running cluster analysis for unknown species together with the data matrix of known published Longidorus species.

  • Longidorus biformis n sp and l glycines n sp nematoda longidoridae two amphimictic species from arkansas
    Journal of Nematology, 2004
    Co-Authors: R. T. Robbins
    Abstract:

    Two new amphimictic species of Longidorus were found in Arkansas. Longidorus biformis n. sp., found in the rhizosphere of hardwood trees along streams in sandy soil in 14 Arkansas locations, is characterized by its long body (5.42-9.50 mm), wide expanded flattened head end, head width 20.0 to 26.0 µm, odontostyle 96 to 125 µm, guide ring 29 to 38 µm posterior to the anterior end, elongate conoid tail, and c' = 0.9-2.1. Females with 2 to 11 vetromedian supplement-like structures were found in 2 of 14 populations of this new species. Longidorus biformis n. sp. is closest to L. seinhorsti Peneva, Loof & Brown, 1998 and L. closelongatus Stoyanov, 1964. Among North American species it is closest to L. glycines n. sp. A distinguishing feature of L. biformis n. sp. is the presence of supplement-like organs in some females. Longidorus glycines n. sp., found in soybean microplots at the Main Research Station, Fayetteville, Arkansas, is characterized by its long body (6.14-8.31 mm), wide offset flattened head end, head width 20.3 to 23.3 µm, odontostyle 87.3 to 99.5 µm, guide ring 22.3 to 26.4 µm posterior to the anterior end, short conoid tail with rounded terminus, and c' = 0.9-1.4. Longidorus glycines n. sp. is closest to L. lusitanicus Macara, 1985. Among North American species it is close to L. biformis n. sp., L. breviannulatus Norton and Hoffman, 1975, and L. crassus Thorne, 1964. Both new species are believed to have four juvenile stages; the first stage was not found for L. biformis n. sp.

  • phylogenetic relationships and genetic variation in Longidorus and xiphinema species nematoda longidoridae using its1 sequences of nuclear ribosomal dna
    Journal of Nematology, 2004
    Co-Authors: Weimin Ye, Allen L. Szalanski, R. T. Robbins
    Abstract:

    Genetic analyses using DNA sequences of nuclear ribosomal DNA ITS1 were conducted to determine the extent of genetic variation within and among Longidorus and Xiphinema species. DNA sequences were obtained from samples collected from Arkansas, California and Australia as well as 4 Xiphinema DNA sequences from GenBank. The sequences of the ITS1 region including the 3' end of the 18S rDNA gene and the 5' end of the 5.8S rDNA gene ranged from 1020 bp to 1244 bp for the 9 Longidorus species, and from 870 bp to 1354 bp for the 7 Xiphinema species. Nucleotide frequencies were: A = 25.5%, C = 21.0%, G = 26.4%, and T = 27.1%. Genetic variation between the two genera had a maximum divergence of 38.6% between X. chambersi and L. crassus. Genetic variation among Xiphinema species ranged from 3.8% between X. diversicaudatum and X. bakeri to 29.9% between X. chambersi and X. italiae. Within Longidorus, genetic variation ranged from 8.9% between L. crassus and L. grandis to 32.4% between L. fragilis and L. diadecturus. Intraspecific genetic variation in X. americanum sensu lato ranged from 0.3% to 1.9%, while genetic variation in L. diadecturus had 0.8% and L. biformis ranged from 0.6% to 10.9%. Identical sequences were obtained between the two populations of L. grandis, and between the two populations of X. bakeri. Phylogenetic analyses based on the ITS1 DNA sequence data were conducted on each genus separately using both maximum parsimony and maximum likelihood analysis. Among the Longidorus taxa, 4 subgroups are supported: L. grandis, L. crassus, and L. elongatus are in one cluster; L. biformis and L. paralongicaudatus are in a second cluster; L. fragilis and L. breviannulatus are in a third cluster; and L. diadecturus is in a fourth cluster. Among the Xiphinema taxa, 3 subgroups are supported: X. americanum with X. chambersi, X. bakeri with X. diversicaudatum, and X. italiae and X. vuittenezi forming a sister group with X. index. The relationships observed in this study correspond to previous genera and species defined by morphology.

Vlada Peneva - One of the best experts on this subject based on the ideXlab platform.

  • molecular and morphological characterisation of Longidorus polyae sp n and l pisi edward misra singh 1964 dorylaimida longidoridae from bulgaria
    ZooKeys, 2019
    Co-Authors: Stela Lazarova, Milka Elshishka, Jingwu Zheng, Francesca De Luca, Georgi Radoslavov, Lydmila Lozanova, Peter Hristov, Alexander Mladenov, Elena Fanelli, Vlada Peneva
    Abstract:

    Longidoruspolyaesp. n., a bisexual nematode species found in the rhizosphere of pear tree (Pyruscommunis L.), is described and characterised using an integrative approach. The new species has a female body length of 6.8-9.1 mm; a comparatively long odontostyle (114.0-127.5 μm); a narrow lip region (14.0-15.5 μm), anteriorly flattened and almost continuous with the body profile; pocket-like amphidial pouches long, deeply bilobed, and slightly asymmetrical, a guide ring at 37-42 μm from the anterior end; normal arrangement of pharyngeal glands; and a short bluntly rounded to hemispherical tail. Four juvenile stages identified: the first stage with a digitate tail, and the second and subsequent stages with a bluntly rounded tail. Males have one adcloacal pair and a row of 10 or 11 single ventromedian supplements; spicules 71.0-74.5 μm long. Based on morphometric data, the new species belongs to a group of species spread over Europe (L.arthensis, L.silvae, L.uroshis,), Iran (L.kheirii), and Syria (L.pauli), which share common characters such as amphidial fovea, lip region and tail shapes, similar odontostyle and body length, and similar first-stage juvenile tail shape. Codes for identifying the new species are A5, B2, C34, D3, E3, F45, G12, H1, I2, J1, K7. The phylogenetic analysis based on D2-D3 expansion domains of the rRNA gene revealed that the new species has the closest relationships with L.athesinus from Italy and three unidentified Longidorus spp. from USA (Longidorus sp. 1, Longidorus sp. 2, and Longidorus sp. 6). New morphometric and molecular data (18S rRNA gene, ITS1-5.8S-ITS2 regions and D2-D3 28S rRNA gene sequences) for three populations of L.pisi from Bulgaria were obtained and variations between populations are discussed.

  • Description of Longidorus cheni sp. n. (Nematoda, Longidoridae) from China
    Pensoft Publishers, 2018
    Co-Authors: Eda Marie Barsalote, Stela Lazarova, Vlada Peneva, Hoa Thi Pham, Jingwu Zheng
    Abstract:

    Longidorus cheni sp. n., an amphimictic species recovered from the rhizosphere of Larix principis-rupprechtii and Pyracantha fortuneana in Shanxi and Beijing, China, is described and illustrated. The taxonomic position of L. cheni sp. n. among other species within the genus was elucidated using morphometric and molecular data, and phylogenetic relationships were inferred using D2–D3 expansion domains of 28S and 18S rRNA genes by Bayesian Inference (BI) method. The new species is characterised by females with a medium body size (L = 4.9–6.6 mm), a lip region slightly expanded, broadly rounded frontally and laterally, the amphidial fovea broad and symmetrically bilobed at base, odontostyle long and slender (143–168 μm), odonthophore slightly swollen at the base, tail short bluntly conoid to rounded. Guide ring located far posterior from the oral aperture (70–93 μm). Males with two ad-cloacal pairs of supplements preceded by a row of 10–14 ventromedian supplements, with robust spicules measuring 111–126 μm along the median line. Three juvenile stages were present, tail shape of J1 elongate conoid while in J2 and J3 the tail gradually becomes bluntly rounded. Codes for identifying the new species are: A6-B3-C5-D2-E2-F3-G1-H1-I2-J2-K2. Longidorus cheni sp. n. belongs to a group of species with a guide ring at the mid-odontostyle position that have a predominantly Asiatic origin. It differs from all of them by a combination of morphological characters and unique sequences of partial 18S and D2–D3 region of 28S rRNA genes. The percentage dissimilarities in partial 18S and D2–D3 28S rRNA genes of L. cheni to the closest species (L. litchii, L. fangi, L. jonesi and L. juglans) were 1.5 %–1.8 % and 16.8–18.3 %, respectively

  • the morphological and molecular identity of Longidorus piceicola liskova robbins brown 1997 from romania nematoda dorylaimida
    ZooKeys, 2017
    Co-Authors: Mariana Groza, Milka Elshishka, Stela Lazarova, Francesca De Luca, Georgi Radoslavov, Peter Hristov, Elena Fanelli, Mihaela Coman, Vlada Peneva
    Abstract:

    Longidorus piceicola, a new geographical and host record from Romania, was described and illustrated on the basis of two populations originating from a coniferous and a deciduous forest. The main morphological characters of specimens from Romania correspond very well with the type material collected from the soil around Picea abies L. (Slovakia) except for the shorter body and tail. The D2-D3 fragment of 28S rDNA from both populations was amplified and sequenced, and the sequences were identical to L. piceicola sequence from Slovakia. The partial 18S-ITS1-5.8S-ITS2 rDNA regions from one of the populations were sequenced for the first time. The evolutionary relationships between L. piceicola and the closest species L. intermedius based on D2-D3 sequence divergence and single-nucleotide polymorphisms are discussed. Although having very low sequence dissimilarity (0.3-0.9 %) both species have distinct morphology and biology. Longidorus piceicola differs from L. intermedius in having a much longer odontostyle, body, distance anterior end - guide ring, a wider lip region, more ventromedian supplements (11 vs 5-7) in the male, and develops through four rather than three juvenile stages. Furthermore, L. piceicola occurs more frequently in association with conifers, while L. intermedius is found mainly in oak forests.

  • morphology and distribution of Longidorus euonymus nematoda from romania
    Scientific Papers - Series A Agronomy, 2014
    Co-Authors: Mariana Groza, Stela Lazarova, Ioan Rosca, Vlada Peneva
    Abstract:

    Longidorus euonymus Mali et Hooper, 1974 from three localities in Romania is described and illustrated on the basis of adult and juvenile specimens. This species was recovered in association with barley, strawberry, blackberry, cherry, sour cherry, plum and represents a new geographical record for Romanian longidorid fauna.

  • description of Longidorus cholevae sp n nematoda dorylaimida from a riparian habitat in the rila mountains bulgaria
    ZooKeys, 2013
    Co-Authors: Vlada Peneva, Stela Lazarova, Francesca De Luca, D J F Brown
    Abstract:

    A description is provided of Longidorus cholevae sp. n., a bisexual species associated with wild cherry (Prunus avium L.) from the Rila Mountains, Bulgaria. The position of L. cholevae sp. n. among other species of the genus was elucidated by using morphological and molecular data. Phylogenetic analyses were performed of D2-D3 expansion domains of the 28S rRNA and the partial ITS1 containing regions by Neighbor-Joining, Maximum Likelihood and Bayesian Inference methods. The species is characterised by a female body length of 6.1-8.1 mm; long odontostyle (106-129 μm); lip region wide (21.5-24 μm) rounded and continuous with the body profile; amphidial pouches short and wide, funnel-shaped; a posteriorly situated guide ring (30-37 μm); normal arrangement of pharyngeal glands, and short bluntly rounded to hemispherical tail. Four juvenile stages indentified, first stage with elongate conoid tail. Males with 2-4 adanal pairs and a row of 11-13 single ventromedian supplements, spicules 96-120 μm long. Based both on morphological and molecular data the new species appearred to be the most similar witha group of species distributed in Europe sharing common charcters such as amphidial fovea, lip region and tail shapes, and having similar odontostyle and body length: L. poessneckensis, L. caespiticola, L. macrososma, L. helveticus, L. carniolensis and L. pius. An updated list of Longidorus species and a partial polytomous keys to the Longidorus species with long odontostyle (code A45) and short tail (code H1) are provided.

Related terms

Longidorus - 15 days free trial to Access Experts & Abstracts