The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Allen W Miller - One of the best experts on this subject based on the ideXlab platform.
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Blackwell Science, LtdPathogen profile Barley yellow dwarf virus: Luteoviridae or Tombusviridae?
2014Co-Authors: Allen W Miller, S I Jun, Randy BeckettAbstract:Barley yellow dwarf virus (BYDV), the most economically important virus of small grains, features highly specialised relationships with its aphid vectors, a plethora of novel translation mechanisms mediated by long–distance RNA interactions, and an ambiguous taxonomic status. The structural and movement proteins of BYDV that confer aphid transmission and phloem-limitation properties resemble those of the Luteoviridae, the family in which BYDV is classified. In contrast, many genes and cis-acting signals involved in replication and gene expression most closely resemble those of the Tombusviridae. Taxonomy: BYDV is in genus Luteovirus, family Luteoviri-dae. BYDV includes at least two serotypes or viruses: BYDV-PAV and BYDV-MAV. The former BYDV-RPV is now Cereal yellow dwarf virus-RPV (CYDV-RPV). CYDV is in genus Polerovirus, fam-ily Luteoviridae. Genus Luteovirus shares many features with family Tombusviridae. Physical properties: ∼25 nm icosahedral (T = 3) virions. One major (22 kDa) and one minor (50–55 kDa) coat protein. 5.6–5.8 kb positive sense RNA genome with no 5′-cap and no poly(A) tail. Host range: Most grasses. Most important in oats, barley and wheat. Also infects maize and rice. Symptoms: Yellowing and dwarfing in barley, stunting in wheat; reddening, yellowing and blasting in oats. Some isolates cause leaf notching and curling. Key attractions: Model for the study of circulative trans-mission of aphid-transmitted viruses. Plethora of unusual trans-lation mechanisms. Evidence of recombination in recent evolutionary history creates taxonomic ambiguity. Economically important virus of wheat, barley and oats, worldwide. Useful websites/meetings: International symposium
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interfamilial recombination between viruses led to acquisition of a novel translation enhancing rna element that allows resistance breaking
New Phytologist, 2014Co-Authors: Manuel Miras, Allen W Miller, Raquel Navarro Sempere, Jelena J Kraft, Miguel A Aranda, Veronica TrunigerAbstract:Many plant viruses depend on functional RNA elements, called 3'-UTR cap-independent translation enhancers (3'-CITEs), for translation of their RNAs. In this manuscript we provide direct proof for the existing hypothesis that 3'-CITEs are modular and transferable by recombination in nature, and that this is associated with an advantage for the created virus. By characterizing a newly identified Melon necrotic spot virus (MNSV; Tombusviridae) isolate, which is able to overcome eukaryotic translation initiation factor 4E (eIF4E)-mediated resistance, we found that it contains a 55 nucleotide insertion in its 3'-UTR. We provide strong evidence that this insertion was acquired by interfamilial recombination with the 3'-UTR of an Asiatic Cucurbit aphid-borne yellows virus (CABYV; Luteoviridae). By constructing chimeric viruses, we showed that this recombined sequence is responsible for resistance breaking. Analysis of the translational efficiency of reporter constructs showed that this sequence functions as a novel 3'-CITE in both resistant and susceptible plants, being essential for translation control in resistant plants. In conclusion, we showed that a recombination event between two clearly identified viruses from different families led to the transfer of exactly the sequence corresponding to a functional RNA element, giving rise to a new isolate with the capacity to infect an otherwise nonsusceptible host.
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the complete nucleotide sequence of the genome of barley yellow dwarf virus rmv reveals it to be a new polerovirus distantly related to other yellow dwarf viruses
Frontiers in Microbiology, 2013Co-Authors: Elizabeth N Krueger, Randy J Beckett, Stewart M Gray, Allen W MillerAbstract:The yellow dwarf viruses (YDVs) of the Luteoviridae family represent the most widespread group of cereal viruses worldwide. They include the Barley yellow dwarf viruses (BYDVs) of genus Luteovirus, the Cereal yellow dwarf viruses (CYDVs) and Wheat yellow dwarf virus (WYDV) of genus Polerovirus. All of these viruses are obligately aphid transmitted and phloem-limited. The first described YDVs (initially all called BYDV) were classified by their most efficient vector. One of these viruses, BYDV-RMV, is transmitted most efficiently by the corn leaf aphid, Rhopalosiphum maidis. Here we report the complete 5612 nucleotide sequence of the genomic RNA of a Montana isolate of BYDV-RMV (isolate RMV MTFE87, Genbank accession no. KC921392). The sequence revealed that BYDV-RMV is a polerovirus, but it is quite distantly related to the CYDVs or WYDV, which are very closely related to each other. Nor is BYDV-RMV closely related to any other particular polerovirus. Depending on the gene that is compared, different poleroviruses (none of them a YDV) share the most sequence similarity to BYDV-RMV. Because of its distant relationship to other YDVs, and because it commonly infects maize via its vector, R. maidis, we propose that BYDV-RMV be renamed Maize yellow dwarf virus-RMV (MYDV-RMV).
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a peptide that binds the pea aphid gut impedes entry of pea enation mosaic virus into the aphid hemocoel
Virology, 2010Co-Authors: S Sivakumar, Allen W Miller, Wendy O Sparks, Bryony C. BonningAbstract:Abstract Development of ways to block virus transmission by aphids could lead to novel and broad-spectrum means of controlling plant viruses. Viruses in the Luteoviridae enhanced are obligately transmitted by aphids in a persistent manner that requires virion accumulation in the aphid hemocoel. To enter the hemocoel, the virion must bind and traverse the aphid gut epithelium. By screening a phage display library, we identified a 12-residue gut binding peptide (GBP3.1) that binds to the midgut and hindgut of the pea aphid Acyrthosiphon pisum . Binding was confirmed by labeling the aphid gut with a GBP3.1–green fluorescent protein fusion. GBP3.1 reduced uptake of Pea enation mosaic virus (Luteoviridae) from the pea aphid gut into the hemocoel. GBP3.1 also bound to the gut epithelia of the green peach aphid and the soybean aphid. These results suggest a novel strategy for inhibiting plant virus transmission by at least three major aphid pest species.
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baculovirus expressed virus like particles of pea enation mosaic virus vary in size and encapsidate baculovirus mrnas
Virus Research, 2009Co-Authors: S Sivakumar, Allen W Miller, Zhaohui Wang, Robert L Harrison, Bryony C. BonningAbstract:Abstract Pea enation mosaic virus (PEMV: family Luteoviridae ) is transmitted in a persistent, circulative manner by aphids. We inserted cDNAs encoding the structural proteins of PEMV, the coat protein (CP) and coat protein-read through domain (CPRT) into the genome of the baculovirus Autographa californica multiple nucleopolyhedrovirus with and without a histidine tag or an upstream Kozak consensus sequence. The Sf21 cell line provided the highest level of CP expression of the cell lines tested and resulted in production of virus-like particles (VLPs). The CPRT was not detected in recombinant baculovirus-infected cells by Western blot. Addition of a Kozak sequence increased the yield of baculovirus produced VLPs. Baculovirus-expressed VLPs purified on a nickel NTA column were of variable size (13–30 nm) and contained CP mRNA. The purified VLPs were also shown by RT-PCR to contain 70% of 154 baculovirus mRNAs, indicative of non-specific RNA encapsidation in the absence of viral RNA replication. When fed to the pea aphid, Acyrthosiphon pisum (Harris), the VLPs entered the aphid hemocoel, demonstrating that CPRT is not required for uptake of PEMV from the aphid gut. Baculovirus expression of PEMV VLPs provides a useful tool for future analysis of RNA encapsidation requirements and molecular aphid–virus interactions.
Veronique Brault - One of the best experts on this subject based on the ideXlab platform.
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transmission of turnip yellows virus by myzus persicae is reduced by feeding aphids on double stranded rna targeting the ephrin receptor protein
Frontiers in Microbiology, 2018Co-Authors: Michael Mulot, Baptiste Monsion, Sylvaine Boissinot, Maryam Rastegar, Sophie Meyer, Nicole Bochet, Veronique BraultAbstract:Aphid-transmitted plant viruses are a threat for major crops causing massive economic loss worldwide. Members in the Luteoviridae family are transmitted by aphids in a circulative and non-replicative mode. Virions are acquired by aphids when ingesting sap from infected plants and are transported through the gut and the accessory salivary gland (ASG) cells by a transcytosis mechanism relying on virus-specific receptors largely unknown. Once released into the salivary canal, virions are inoculated to plants, together with saliva, during a subsequent feeding. In this paper, we bring in vivo evidence that the membrane-bound Ephrin receptor (Eph) is a novel aphid protein involved in the transmission of the Turnip yellows virus (TuYV, Polerovirus genus, Luteoviridae family) by Myzus persicae. The minor capsid protein of TuYV, essential for aphid transmission, was able to bind the external domain of Eph in yeast. Feeding M. persicae on in planta- or in vitro-synthesized dsRNA targeting Eph-mRNA (dsRNAEph) did not affect aphid feeding behavior but reduced accumulation of TuYV genomes in the aphid's body. Consequently, TuYV transmission efficiency by the dsRNAEph-treated aphids was reproducibly inhibited and we brought evidence that Eph is likely involved in intestinal uptake of the virion. The inhibition of virus uptake after dsRNAEph acquisition was also observed for two other poleroviruses transmitted by M. persicae, suggesting a broader role of Eph in polerovirus transmission. Finally, dsRNAEph acquisition by aphids did not affect nymph production. These results pave the way toward an ecologically safe alternative of insecticide treatments that are used to lower aphid populations and reduce polerovirus damages.
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Targeted disruption of aphid transmission: a vision for the management of crop diseases caused by Luteoviridae members
Current Opinion in Virology, 2018Co-Authors: Michelle Heck, Veronique BraultAbstract:Viruses in the Luteoviridae cause plant diseases that are notoriously difficult to manage. Referred to as luteovirids, these single stranded, positive-sense RNA viruses are transmitted by aphids in a circulative, non-propagative manner. This review highlights new potential strategies to control luteovirid disease by blocking virus transmission by aphids. These include: first, interfering with aphid–virus interactions to inhibit virus acquisition by aphids, second, manipulating the host plant to block virus acquisition and inoculation, and third, rapid identification of efficient vector populations for the delivery of targeted control strategies. Translation of these methods to the field requires further advances in basic and translational research and the development of new tools to study the tritrophic interactions among plants, luteovirids, and aphids.
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both structural and non structural forms of the readthrough protein of cucurbit aphid borne yellows virus are essential for efficient systemic infection of plants
PLOS ONE, 2014Co-Authors: Baptiste Monsion, Sylvaine Boissinot, Veronique Brault, Monique Erdinger, Veronique ZieglergraffAbstract:Cucurbit aphid-borne yellows virus (CABYV) is a polerovirus (Luteoviridae family) with a capsid composed of the major coat protein and a minor component referred to as the readthrough protein (RT). Two forms of the RT were reported: a full-length protein of 74 kDa detected in infected plants and a truncated form of 55 kDa (RT*) incorporated into virions. Both forms were detected in CABYV-infected plants. To clarify the specific roles of each protein in the viral cycle, we generated by deletion a polerovirus mutant able to synthesize only the RT* which is incorporated into the particle. This mutant was unable to move systemically from inoculated leaves inferring that the C-terminal half of the RT is required for efficient long-distance transport of CABYV. Among a collection of CABYV mutants bearing point mutations in the central domain of the RT, we obtained a mutant impaired in the correct processing of the RT which does not produce the RT*. This mutant accumulated very poorly in upper non-inoculated leaves, suggesting that the RT* has a functional role in long-distance movement of CABYV. Taken together, these results infer that both RT proteins are required for an efficient CABYV movement.
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Identification and characterisation of host factors involved in plant virus long distance movement
2009Co-Authors: S. Chapuis, Sylvaine Boissinot, Veronique Brault, Frederic Revers, Véronique Ziegler-graffAbstract:Poleroviruses are an agronomically important genus of plant vlruses (Luteoviridae family) tbat infect a wide range of plants. lnterestingly, tbey are restricted to the phloem tissue and tberefore provide a unique model for studying long distance movement of viruses in plants. Moreover the mechanism underlying this transport, although essential for plant viruses, has never been extensively investigated. The aim of this project is to identify phloem specifie genes, especially those from companion cells that are either deregulated by viral infection or en code proteins that interact directly with viral proteins involved in transport. This project is based on the specifie isolation of companlOn cells by laser capture microdissection that will be used in two different types of experiments: - the construction of a specifie companion cells cDNA library that will serve as a pray in the yeast two hybrid system to screen for proteins interacting with viral factors (bait) involved in long distance movement. - a comparative transcriptional analysis of Arabidopsis thaliana cDNA microarrays using healthy and companion cells infected by BWYV (beet western yellows virus). After hybridization of microarrays, data analysis will supply a set of deregulated phloem genes. Alteration in gene expression will be confirmed and the best candidates identified by quantitative RT-PCR will be further analyzed. The role of a few selected candidate genes identified either by transcriptomic or biochemical (yeast two hybrid) approach will be evaluated regarding virus long distance movement. As this project only recently started, this poster will mainly focus on technical design and optimization of sample preparation and microdissection.
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studies on the role of the minor capsid protein in transport of beet western yellows virus through myzus persicae
Journal of General Virology, 2001Co-Authors: Catherine Reinbold, F E Gildow, E Herrbach, Marcos Cesar Goncalves, Veronique Zieglergraff, J F J M Van Den Heuvel, Veronique BraultAbstract:Beet western yellows virus (BWYV), family Luteoviridae, is an icosahedral plant virus which is strictly transmitted by aphids in a persistent and circulative manner. Virions cross two cellular barriers in the aphid by receptor-based mechanisms involving endocytosis and exocytosis. Particles are first transported across intestinal cells into the haemolymph and then across accessory salivary gland cells for delivery to the plant via saliva. We identified the midgut part of the digestive tract as the site of intestinal passage by BWYV virions. To analyse the role in transmission of the minor capsid component, the readthrough (RT) protein, the fate of a BWYV RT-deficient non-transmissible mutant was followed by transmission electron microscopy in the vector Myzus persicae. This mutant was observed in the gut lumen but was never found inside midgut cells. However, virion aggregates were detected in the basal lamina of midgut cells when BWYV antiserum was microinjected into the haemolymph. The presence of virions in the haemolymph was confirmed by a sensitive molecular technique for detecting viral RNA. Thus, transport of the mutant virions through intestinal cells occurred but at a low frequency. Even when microinjected into the haemolymph, the RT protein mutant was never detected near or in the accessory salivary gland cells. We conclude that the RT protein is not strictly required for the transport of virus particles through midgut cells, but is necessary for the maintenance of virions in the haemolymph and their passage through accessory salivary gland cells.
Randy J Beckett - One of the best experts on this subject based on the ideXlab platform.
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the complete nucleotide sequence of the genome of barley yellow dwarf virus rmv reveals it to be a new polerovirus distantly related to other yellow dwarf viruses
Frontiers in Microbiology, 2013Co-Authors: Elizabeth N Krueger, Randy J Beckett, Stewart M Gray, Allen W MillerAbstract:The yellow dwarf viruses (YDVs) of the Luteoviridae family represent the most widespread group of cereal viruses worldwide. They include the Barley yellow dwarf viruses (BYDVs) of genus Luteovirus, the Cereal yellow dwarf viruses (CYDVs) and Wheat yellow dwarf virus (WYDV) of genus Polerovirus. All of these viruses are obligately aphid transmitted and phloem-limited. The first described YDVs (initially all called BYDV) were classified by their most efficient vector. One of these viruses, BYDV-RMV, is transmitted most efficiently by the corn leaf aphid, Rhopalosiphum maidis. Here we report the complete 5612 nucleotide sequence of the genomic RNA of a Montana isolate of BYDV-RMV (isolate RMV MTFE87, Genbank accession no. KC921392). The sequence revealed that BYDV-RMV is a polerovirus, but it is quite distantly related to the CYDVs or WYDV, which are very closely related to each other. Nor is BYDV-RMV closely related to any other particular polerovirus. Depending on the gene that is compared, different poleroviruses (none of them a YDV) share the most sequence similarity to BYDV-RMV. Because of its distant relationship to other YDVs, and because it commonly infects maize via its vector, R. maidis, we propose that BYDV-RMV be renamed Maize yellow dwarf virus-RMV (MYDV-RMV).
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barley yellow dwarf virus Luteoviridae or tombusviridae
Molecular Plant Pathology, 2002Co-Authors: Allen W Miller, Randy J BeckettAbstract:Summary Barley yellow dwarf virus (BYDV), the most economically important virus of small grains, features highly specialised relationships with its aphid vectors, a plethora of novel translation mechanisms mediated by long–distance RNA interactions, and an ambiguous taxonomic status. The structural and movement proteins of BYDV that confer aphid transmission and phloem-limitation properties resemble those of the Luteoviridae , the family in which BYDV is classified. In contrast, many genes and cis -acting signals involved in replication and gene expression most closely resemble those of the Tombusviridae . Taxonomy: BYDV is in genus Luteovirus, family Luteoviridae. BYDV includes at least two serotypes or viruses: BYDV-PAV and BYDV-MAV. The former BYDV-RPV is now Cereal yellow dwarf virus-RPV (CYDV-RPV). CYDV is in genus Polerovirus, family Luteoviridae. Genus Luteovirus shares many features with family Tombusviridae. Physical properties: ∼25 nm icosahedral (T = 3) virions. One major (22 kDa) and one minor (50–55 kDa) coat protein. 5.6–5.8 kb positive sense RNA genome with no 5′-cap and no poly(A) tail. Host range: Most grasses. Most important in oats, barley and wheat. Also infects maize and rice. Symptoms: Yellowing and dwarfing in barley, stunting in wheat; reddening, yellowing and blasting in oats. Some isolates cause leaf notching and curling. Key attractions: Model for the study of circulative transmission of aphid-transmitted viruses. Plethora of unusual translation mechanisms. Evidence of recombination in recent evolutionary history creates taxonomic ambiguity. Economically important virus of wheat, barley and oats, worldwide. Useful websites/meetings: International symposium: ‘Barley Yellow Dwarf Disease: Recent Advances and Future Strategies’, CIMMYT, El Batan, Mexico, 1–5 September 2002, http://www.cimmyt.cgiar.org/Research/wheat/Conf_BYD_02/invitation.htm http://www.cimmyt.org/Research/wheat/BYDVNEWS/htm/BYDVNEWS.htm Aphid transmission animation: http://www.ppws.vt.edu/~sforza/tmv/bydv_aph.html
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the 3 terminal structure required for replication of barley yellow dwarf virus rna contains an embedded 3 end
Virology, 2002Co-Authors: Gennadiy Koev, Randy J Beckett, Sijun Liu, Allen W MillerAbstract:Abstract We determined the 3′-terminal primary and secondary structures required for replication of Barley yellow dwarf virus (BYDV) RNA in oat protoplasts. Computer predictions, nuclease probing, phylogenetic comparisons, and replication assays of specific mutants and chimeras revealed that the 3′-terminal 109 nucleotides (nt) form a structure with three to four stem-loops followed by a coaxially stacked helix incorporating the last four nt [(A/U)CCC]. Sequences upstream of the 109-nt region also contributed to RNA accumulation. The base-pairing but not the sequences or bulges in the stems were essential for replication, but any changes to the 3′-terminal helix destroyed replication. The two 3′-proximal tetraloops tolerated all changes, but the two 3′-distal tetraloops gave most efficient replication if they fit the GNRA consensus. A mutant lacking the 3′-proximal stem-loop produced elevated levels of less-than-full-length minus strands, and no (+) strand. We propose that a “pocket” structure is the origin of (−)-strand synthesis, which is negatively regulated by the inaccessible conformation of the 3′ terminus, thus favoring a high (+)/(−) ratio. This 3′ structure and the polymerase homologies suggest that genus Luteovirus is more closely related to the Tombusviridae family than to other Luteoviridae genera.
Stewart M Gray - One of the best experts on this subject based on the ideXlab platform.
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the interaction dynamics of two potato leafroll virus movement proteins affects their localization to the outer membranes of mitochondria and plastids
Viruses, 2018Co-Authors: Stacy L Deblasio, Michael J Maccoss, Richard S Johnson, Ana Rita Rebelo, Stewart M Gray, Michelle HeckAbstract:The Luteoviridae is an agriculturally important family of viruses whose replication and transport are restricted to plant phloem. Their genomes encode for four proteins that regulate viral movement. These include two structural proteins that make up the capsid and two non-structural proteins known as P3a and P17. Little is known about how these proteins interact with each other and the host to coordinate virus movement within and between cells. We used quantitative, affinity purification-mass spectrometry to show that the P3a protein of Potato leafroll virus complexes with virus and that this interaction is partially dependent on P17. Bimolecular complementation assays (BiFC) were used to validate that P3a and P17 self-interact as well as directly interact with each other. Co-localization with fluorescent-based organelle markers demonstrates that P3a directs P17 to the mitochondrial outer membrane while P17 regulates the localization of the P3a-P17 heterodimer to plastids. Residues in the C-terminus of P3a were shown to regulate P3a association with host mitochondria by using mutational analysis and also varying BiFC tag orientation. Collectively, our work reveals that the PLRV movement proteins play a game of intracellular hopscotch along host organelles to transport the virus to the cell periphery.
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potato leafroll virus structural proteins manipulate overlapping yet distinct protein interaction networks during infection
Proteomics, 2015Co-Authors: Stacy L Deblasio, Michael J Maccoss, Stewart M Gray, Michelle Cilia, Richard J Johnson, Michelle Sweeney, Alexander V KarasevAbstract:Potato leafroll virus (PLRV) produces a readthrough protein (RTP) via translational readthrough of the coat protein amber stop codon. The RTP functions as a structural component of the virion and as a nonincorporated protein in concert with numerous insect and plant proteins to regulate virus movement/transmission and tissue tropism. Affinity purification coupled to quantitative MS was used to generate protein interaction networks for a PLRV mutant that is unable to produce the read through domain (RTD) and compared to the known wild-type PLRV protein interaction network. By quantifying differences in the protein interaction networks, we identified four distinct classes of PLRV-plant interactions: those plant and nonstructural viral proteins interacting with assembled coat protein (category I); plant proteins in complex with both coat protein and RTD (category II); plant proteins in complex with the RTD (category III); and plant proteins that had higher affinity for virions lacking the RTD (category IV). Proteins identified as interacting with the RTD are potential candidates for regulating viral processes that are mediated by the RTP such as phloem retention and systemic movement and can potentially be useful targets for the development of strategies to prevent infection and/or viral transmission of Luteoviridae species that infect important crop species.
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Circulative, "nonpropagative" virus transmission: an orchestra of virus-, insect-, and plant-derived instruments.
Advances in virus research, 2014Co-Authors: Stewart M Gray, Michelle Cilia, Murad GhanimAbstract:Species of plant viruses within the Luteoviridae, Geminiviridae, and Nanoviridae are transmitted by phloem-feeding insects in a circulative, nonpropagative manner. The precise route of virus movement through the vector can differ across and within virus families, but these viruses all share many biological, biochemical, and ecological features. All share temporal and spatial constraints with respect to transmission efficiency. The viruses also induce physiological changes in their plant hosts resulting in behavioral changes in the insects that optimize the transmission of virus to new hosts. Virus proteins interact with insect, endosymbiont, and plant proteins to orchestrate, directly and indirectly, virus movement in insects and plants to facilitate transmission. Knowledge of these complex interactions allows for the development of new tools to reduce or prevent transmission, to quickly identify important vector populations, and to improve the management of these economically important viruses affecting agricultural and natural plant populations.
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the complete nucleotide sequence of the genome of barley yellow dwarf virus rmv reveals it to be a new polerovirus distantly related to other yellow dwarf viruses
Frontiers in Microbiology, 2013Co-Authors: Elizabeth N Krueger, Randy J Beckett, Stewart M Gray, Allen W MillerAbstract:The yellow dwarf viruses (YDVs) of the Luteoviridae family represent the most widespread group of cereal viruses worldwide. They include the Barley yellow dwarf viruses (BYDVs) of genus Luteovirus, the Cereal yellow dwarf viruses (CYDVs) and Wheat yellow dwarf virus (WYDV) of genus Polerovirus. All of these viruses are obligately aphid transmitted and phloem-limited. The first described YDVs (initially all called BYDV) were classified by their most efficient vector. One of these viruses, BYDV-RMV, is transmitted most efficiently by the corn leaf aphid, Rhopalosiphum maidis. Here we report the complete 5612 nucleotide sequence of the genomic RNA of a Montana isolate of BYDV-RMV (isolate RMV MTFE87, Genbank accession no. KC921392). The sequence revealed that BYDV-RMV is a polerovirus, but it is quite distantly related to the CYDVs or WYDV, which are very closely related to each other. Nor is BYDV-RMV closely related to any other particular polerovirus. Depending on the gene that is compared, different poleroviruses (none of them a YDV) share the most sequence similarity to BYDV-RMV. Because of its distant relationship to other YDVs, and because it commonly infects maize via its vector, R. maidis, we propose that BYDV-RMV be renamed Maize yellow dwarf virus-RMV (MYDV-RMV).
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cross linking measurements of the potato leafroll virus reveal protein interaction topologies required for virion stability aphid transmission and virus plant interactions
Journal of Proteome Research, 2012Co-Authors: Juan D Chavez, Stewart M Gray, Michelle Cilia, Chad R Weisbrod, Jimmy K Eng, James E BruceAbstract:Protein interactions are critical determinants of insect transmission for viruses in the family Luteoviridae. Two luteovirid structural proteins, the capsid protein (CP) and the readthrough protein (RTP), contain multiple functional domains that regulate virus transmission. There is no structural information available for these economically important viruses. We used Protein Interaction Reporter (PIR) technology, a strategy that uses chemical cross-linking and high resolution mass spectrometry, to discover topological features of the Potato leafroll virus (PLRV) CP and RTP that are required for the diverse biological functions of PLRV virions. Four cross-linked sites were repeatedly detected, one linking CP monomers, two within the RTP, and one linking the RTP and CP. Virus mutants with triple amino acid deletions immediately adjacent to or encompassing the cross-linked sites were defective in virion stability, RTP incorporation into the capsid, and aphid transmission. Plants infected with a new, infectio...
Marcos Cesar Goncalves - One of the best experts on this subject based on the ideXlab platform.
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first report of maize yellow mosaic virus infecting maize in brazil
Plant Disease, 2017Co-Authors: Marcos Cesar Goncalves, Marcio Tadeu Godinho, D M T Alvesfreitas, Arvind Varsani, S G RibeiroAbstract:Maize (Zea mays) is a staple crop for human consumption and widely used as a livestock feed worldwide. Therefore, potential new diseases affecting this crop are of major concern. In May 2013, yellow mosaic and dwarfing symptoms were observed in several maize plants in a field located in the rural area of Casa Branca (21° 46′ 26″ S, 47° 5′ 9″ W), Sao Paulo state (Southeastern Brazil). Symptomatic leaves were sampled, and total RNA was isolated using Trizol™ reagent and further purified with PureLink™ RNA Mini Kit spin columns (Invitrogen, USA). A library was prepared with an Illumina TruSeq Stranded Total RNA sample preparation kit with Ribo-Zero Plant and sequenced on an Illumina Hiseq2500 platform at Macrogen Inc, South Korea. De novo assembly using Abyss 1.9 with K-mer = 64 yieldeda contig of 1183 nt with high similarities (92-93%) to several isolates sequences of Maize yellow mosaic virus (MaYMV), a putative new member of the genus Polerovirus, family Luteoviridae, recently reported in China (Chen et a...
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studies on the role of the minor capsid protein in transport of beet western yellows virus through myzus persicae
Journal of General Virology, 2001Co-Authors: Catherine Reinbold, F E Gildow, E Herrbach, Marcos Cesar Goncalves, Veronique Zieglergraff, J F J M Van Den Heuvel, Veronique BraultAbstract:Beet western yellows virus (BWYV), family Luteoviridae, is an icosahedral plant virus which is strictly transmitted by aphids in a persistent and circulative manner. Virions cross two cellular barriers in the aphid by receptor-based mechanisms involving endocytosis and exocytosis. Particles are first transported across intestinal cells into the haemolymph and then across accessory salivary gland cells for delivery to the plant via saliva. We identified the midgut part of the digestive tract as the site of intestinal passage by BWYV virions. To analyse the role in transmission of the minor capsid component, the readthrough (RT) protein, the fate of a BWYV RT-deficient non-transmissible mutant was followed by transmission electron microscopy in the vector Myzus persicae. This mutant was observed in the gut lumen but was never found inside midgut cells. However, virion aggregates were detected in the basal lamina of midgut cells when BWYV antiserum was microinjected into the haemolymph. The presence of virions in the haemolymph was confirmed by a sensitive molecular technique for detecting viral RNA. Thus, transport of the mutant virions through intestinal cells occurred but at a low frequency. Even when microinjected into the haemolymph, the RT protein mutant was never detected near or in the accessory salivary gland cells. We conclude that the RT protein is not strictly required for the transport of virus particles through midgut cells, but is necessary for the maintenance of virions in the haemolymph and their passage through accessory salivary gland cells.
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molecular evidence that sugarcane yellow leaf virus scylv is a member of the Luteoviridae family
Archives of Virology, 2000Co-Authors: Ivan De Godoy Maia, Marcos Cesar Goncalves, Paulo Arruda, Jorge VegaAbstract:A previously uncharacterized virus was reported in southeast Brazil causing a yellowing leaf disease in sugarcane. The virus, termed sugarcane yellow leaf virus (ScYLV), shares features typical of the luteoviruses. To start the molecular characterization of ScYLV, the nucleotide sequence of the coat protein (CP), 17 kDa protein and C-terminus of the RNA-dependent RNA polymerase coding regions was determined from an RT-PCR amplification product. Comparisons showed that the deduced amino acid sequences share a considerable degree of identity and similarity with corresponding sequences of known luteoviruses, thus clearly establishing ScYLV as a member of the family Luteoviridae. The authenticity of the CP open reading frame was confirmed by its expression in Escherichia coli. The recombinant CP positively reacted in immunoblot assays with polyclonal antibodies raised against native ScYLV. Furthermore, phylogenetic analyses also suggest that the 5′ and 3′ coding blocks of the ScYLV genome possess different taxonomic affinities within the Luteoviridae family, as does also the genome of soybean dwarf virus.
