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Melody A. Keena - One of the best experts on this subject based on the ideXlab platform.
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Effects of Temperature on First Instar Lymantria (Lepidoptera: Erebidae) Survival and Development With and Without Food
Environmental Entomology, 2019Co-Authors: Melody A. KeenaAbstract:Lymantria dispar L. and Lymantria monacha (L.) are Eurasian pests that have the potential for accidental introduction via trade into other world areas. Establishment of first instars of Lymantria depends on larvae surviving long enough to disperse and finding suitable hosts. The survival and development of newly hatched Lymantria larvae from nine geographic populations at seven temperatures (1–30°C) held without food, with summer foliage of a preferred or conifer host was determined. There was considerable variation both within and among the Lymantria populations in the survival of larvae at different temperatures when held with and without food. Without food survival declined from about a month at 5°C to a few days at 30°C, following a typical enzymatic kinetic rate function. At 1°C larval survival was less than at 5°C likely because the larvae were susceptible to freezing. Larvae from the one L. monacha population fed and gained weight on the summer foliage, particularly on the conifer, at 10–15°C but < 20% survived for 14 d at 20–30°C. The newly hatched L. dispar larvae from all eight populations fed (at 10–30°C) and developed (at 15–30°C) on the summer foliage of one or both of the hosts. This suggests that they may be able to find adequate food for establishment even if hatch is not synchronous with bud break in the invaded habitat. Survival on the conifer was highest for one Chinese and two European populations of L. dispar, suggesting the ability to utilize conifers is population and not subspecies specific.
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effects of temperature on development of Lymantria dispar asiatica and Lymantria dispar japonica lepidoptera erebidae
Environmental Entomology, 2017Co-Authors: Samita Limbu, Melody A. Keena, Fang Chen, Gericke Cook, Hannah Nadel, Kelli HooverAbstract:Periodic introductions of the Asian subspecies of gypsy moth, Lymantria dispar asiatica Vnukovskij and Lymantria dispar japonica Motschulsky, in North America are threatening forests and interrupting foreign trade. Although Asian gypsy moth has similar morphology to that of European and North American gypsy moth, it has several traits that make it a greater threat, the most important being the flight capability of females. Asian gypsy moth is not yet established in North America; however, infestations have been detected multiple times in Canada and the United States. To facilitate detection and eradication efforts, we evaluated the effect of a range of temperatures on development time, survivorship, and fertility of eight populations of Asian gypsy moth. There were significant impacts of temperature and population on these life history characteristics. The larval developmental rate increased with temperature until it reached an optimum at 29 °C. Larvae experienced significant molting problems at the highest and lowest temperatures tested (10 °C and 30 °C). At 30 °C, female fitness was markedly compromised, as evidenced by reduced fecundity and fertility. This suggests that development and survival of Asian gypsy moth may be limited by summer temperature extremes in the Southern United States. We also determined the degree-day requirements for two critical life stages and two populations of Asian gypsy moth, which represent the extremes in latitude, to predict the timing for biopesticide application and adult trap deployment. Our data will benefit pest managers in developing management strategies, pest risk assessments, and timing for implementation of management tactics.
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geographic isolates of Lymantria dispar multiple nucleopolyhedrovirus genome sequence analysis and pathogenicity against european and asian gypsy moth strains
Journal of Invertebrate Pathology, 2016Co-Authors: Robert L Harrison, Daniel L Rowley, Melody A. KeenaAbstract:Isolates of the baculovirus species Lymantria dispar multiple nucleopolyhedrovirus have been formulated and applied to suppress outbreaks of the gypsy moth, L. dispar. To evaluate the genetic diversity in this species at the genomic level, the genomes of three isolates from Massachusetts, USA (LdMNPV-Ab-a624), Spain (LdMNPV-3054), and Japan (LdMNPV-3041) were sequenced and compared with four previously determined LdMNPV genome sequences. The LdMNPV genome sequences were collinear and contained the same homologous repeats (hrs) and clusters of baculovirus repeat orf (bro) gene family members in the same relative positions in their genomes, although sequence identities in these regions were low. Of 146 non-bro ORFs annotated in the genome of the representative isolate LdMNPV 5-6, 135 ORFs were found in every other LdMNPV genome, including the 37 core genes of Baculoviridae and other genes conserved in genus Alphabaculovirus. Phylogenetic inference with an alignment of the core gene nucleotide sequences grouped isolates 3041 (Japan) and 2161 (Korea) separately from a cluster containing isolates from Europe, North America, and Russia. To examine phenotypic diversity, bioassays were carried out with a selection of isolates against neonate larvae from three European gypsy moth (Lymantria dispar dispar) and three Asian gypsy moth (Lymantria dispar asiatica and Lymantria dispar japonica) colonies. LdMNPV isolates 2161 (Korea), 3029 (Russia), and 3041 (Japan) exhibited a greater degree of pathogenicity against all L. dispar strains than LdMNPV from a sample of Gypchek. This study provides additional information on the genetic diversity of LdMNPV isolates and their activity against the Asian gypsy moth, a potential invasive pest of North American trees and forests.
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differences in wing morphometrics of Lymantria dispar lepidoptera erebidae between populations that vary in female flight capability
Annals of The Entomological Society of America, 2015Co-Authors: Fang Chen, Melody A. KeenaAbstract:All male gypsy moths, Lymantria dispar L., are capable of strong directed flight, but flight in females varies, increasing from west to east geographically across Eurasia. To better understand how the wings differ between female flight capable and flightless strains, a wing morphometric analysis of 821 gypsy moths from eight geographic strains (three Lymantria dispar dispar L., four Lymantria dispar asiatica Vnukovskij, one Lymantria dispar japonica Motschulsky) was performed. Body mass; length and width of both fore- and hindwing; and wing area, aspect, and loads were measured on both sexes from each strain. Gypsy moths were sexually dimorphic; females had a higher wing load, larger aspect ratios, bigger wing area, and heavier body mass than males. Wing loads of females, but not males, differed significantly among geographic strains and were lower in flight capable strains. Wing aspect was less variable within each sex among the strains. Female fore- and hindwing area were both larger in strains with strong directed flight capabilities compared with flightless strains, suggesting both fore- and hindwing areas play significant roles in flight. A logistic regression model using female forewing length and wing load correctly predicted the female flight capability of the source strains >97% of the time and may be a useful tool to use in conjunction with molecular methods for detecting introductions of Asian gypsy moth. None of the male morphometric wing characters were found to reliably predict the female flight capability of the source population.
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classification genetic variation and pathogenicity of Lymantria dispar nucleopolyhedrovirus isolates from asia europe and north america
Journal of Invertebrate Pathology, 2014Co-Authors: Robert L Harrison, Melody A. Keena, Daniel L RowleyAbstract:Abstract Lymantria dispar multiple nucleopolyhedrovirus (LdMNPV) has been formulated and applied to control outbreaks of the gypsy moth, L. dispar. To classify and determine the degree of genetic variation among isolates of L. dispar NPVs from different parts of the range of the gypsy moth, partial sequences of the lef-8, lef-9, and polh genes were determined for Lymantria spp. virus samples from host populations throughout the world. Sequence analysis confirmed that all L. dispar virus samples tested contained isolates of the species Lymantria dispar multiple nucleopolyhedrovirus (Baculoviridae: Alphabaculovirus). Phylogenetic inference based on the lef-8 sequences indicated that the LdMNPV isolates formed two groups, one consisting primarily of isolates from Asia, and one consisting primarily of isolates from Europe and North America. The complete genome sequence was determined for an isolate from the Asian group, LdMNPV-2161 (S. Korea). The LdMNPV-2161 genome was 163,138 bp in length, 2092 bp larger than the previously determined genome of LdMNPV isolate 5–6 (CT, USA). The two genome sequences were co-linear, with an overall nucleotide sequence identity of 97.5% and some differences in ORF content. In droplet-feeding bioassays against neonate L. dispar larvae, isolates LdMNPV-3029 (Virin-ENSh/Russia) and LdMNPV-Ab-a624 (MA, USA) killed neonate larvae with an LC50 values that were 1.8- to 3.2-fold lower than a sample of Gypchek® (CT, USA) and isolates LdMNPV-3041 (Japan) and LdMNPV-2161. This study expands our knowledge about genetic variation among LdMNPV isolates and provides novel information on the distinct groups in which these NPVs occur.
Robert L Harrison - One of the best experts on this subject based on the ideXlab platform.
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geographic isolates of Lymantria dispar multiple nucleopolyhedrovirus genome sequence analysis and pathogenicity against european and asian gypsy moth strains
Journal of Invertebrate Pathology, 2016Co-Authors: Robert L Harrison, Daniel L Rowley, Melody A. KeenaAbstract:Isolates of the baculovirus species Lymantria dispar multiple nucleopolyhedrovirus have been formulated and applied to suppress outbreaks of the gypsy moth, L. dispar. To evaluate the genetic diversity in this species at the genomic level, the genomes of three isolates from Massachusetts, USA (LdMNPV-Ab-a624), Spain (LdMNPV-3054), and Japan (LdMNPV-3041) were sequenced and compared with four previously determined LdMNPV genome sequences. The LdMNPV genome sequences were collinear and contained the same homologous repeats (hrs) and clusters of baculovirus repeat orf (bro) gene family members in the same relative positions in their genomes, although sequence identities in these regions were low. Of 146 non-bro ORFs annotated in the genome of the representative isolate LdMNPV 5-6, 135 ORFs were found in every other LdMNPV genome, including the 37 core genes of Baculoviridae and other genes conserved in genus Alphabaculovirus. Phylogenetic inference with an alignment of the core gene nucleotide sequences grouped isolates 3041 (Japan) and 2161 (Korea) separately from a cluster containing isolates from Europe, North America, and Russia. To examine phenotypic diversity, bioassays were carried out with a selection of isolates against neonate larvae from three European gypsy moth (Lymantria dispar dispar) and three Asian gypsy moth (Lymantria dispar asiatica and Lymantria dispar japonica) colonies. LdMNPV isolates 2161 (Korea), 3029 (Russia), and 3041 (Japan) exhibited a greater degree of pathogenicity against all L. dispar strains than LdMNPV from a sample of Gypchek. This study provides additional information on the genetic diversity of LdMNPV isolates and their activity against the Asian gypsy moth, a potential invasive pest of North American trees and forests.
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complete genome sequence of the strain of Lymantria dispar multiple nucleopolyhedrovirus found in the gypsy moth biopesticide virin ensh
Genome Announcements, 2015Co-Authors: Robert L Harrison, Daniel L RowleyAbstract:ABSTRACT We report the genome sequence of an alphabaculovirus from the gypsy moth (Lymantria dispar) biopesticide Virin-ENSh. The genome sequence is 161,712 bp, and its structure and sequence similarity indicate that the virus used in Virin-ENSh is a strain of the species Lymantria dispar multiple nucleopolyhedrovirus.
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Lymantria dispar iflavirus 1 ldiv1 a new model to study iflaviral persistence in lepidopterans
Journal of General Virology, 2014Co-Authors: Jimena Carrillotripp, Robert L Harrison, Elizabeth N Krueger, Amy L Toth, Allen W Miller, Bryony C BonningAbstract:The cell line IPLB-LD-652Y, derived from the gypsy moth (Lymantria dispar L.), is routinely used to study interactions between viruses and insect hosts. Here we report the full genome sequence and biological characteristics of a small RNA virus, designated Lymantria dispar iflavirus 1 (LdIV1), that was discovered to persistently infect IPLB-LD-652Y. LdIV1 belongs to the genus Iflavirus. LdIV1 formed icosahedral particles of approx. 30 nm in diameter and contained a 10 044 nt polyadenylated, positive-sense RNA genome encoding a predicted polyprotein of 2980 aa. LdIV1 was induced by a viral suppressor of RNA silencing, suggesting that acute infection is restricted by RNA interference (RNAi). We detected LdIV1 in all tested tissues of gypsy-moth larvae and adults, but the virus was absent from other L. dispar-derived cell lines. We confirmed LdIV1 infectivity in two of these cell lines (IPLB-LD-652 and IPLB-LdFB). Our results provide a novel system to explore persistent infections in lepidopterans and a new model for the study of iflaviruses, a rapidly expanding group of viruses, many of which covertly infect their hosts.
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classification genetic variation and pathogenicity of Lymantria dispar nucleopolyhedrovirus isolates from asia europe and north america
Journal of Invertebrate Pathology, 2014Co-Authors: Robert L Harrison, Melody A. Keena, Daniel L RowleyAbstract:Abstract Lymantria dispar multiple nucleopolyhedrovirus (LdMNPV) has been formulated and applied to control outbreaks of the gypsy moth, L. dispar. To classify and determine the degree of genetic variation among isolates of L. dispar NPVs from different parts of the range of the gypsy moth, partial sequences of the lef-8, lef-9, and polh genes were determined for Lymantria spp. virus samples from host populations throughout the world. Sequence analysis confirmed that all L. dispar virus samples tested contained isolates of the species Lymantria dispar multiple nucleopolyhedrovirus (Baculoviridae: Alphabaculovirus). Phylogenetic inference based on the lef-8 sequences indicated that the LdMNPV isolates formed two groups, one consisting primarily of isolates from Asia, and one consisting primarily of isolates from Europe and North America. The complete genome sequence was determined for an isolate from the Asian group, LdMNPV-2161 (S. Korea). The LdMNPV-2161 genome was 163,138 bp in length, 2092 bp larger than the previously determined genome of LdMNPV isolate 5–6 (CT, USA). The two genome sequences were co-linear, with an overall nucleotide sequence identity of 97.5% and some differences in ORF content. In droplet-feeding bioassays against neonate L. dispar larvae, isolates LdMNPV-3029 (Virin-ENSh/Russia) and LdMNPV-Ab-a624 (MA, USA) killed neonate larvae with an LC50 values that were 1.8- to 3.2-fold lower than a sample of Gypchek® (CT, USA) and isolates LdMNPV-3041 (Japan) and LdMNPV-2161. This study expands our knowledge about genetic variation among LdMNPV isolates and provides novel information on the distinct groups in which these NPVs occur.
Daniel L Rowley - One of the best experts on this subject based on the ideXlab platform.
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geographic isolates of Lymantria dispar multiple nucleopolyhedrovirus genome sequence analysis and pathogenicity against european and asian gypsy moth strains
Journal of Invertebrate Pathology, 2016Co-Authors: Robert L Harrison, Daniel L Rowley, Melody A. KeenaAbstract:Isolates of the baculovirus species Lymantria dispar multiple nucleopolyhedrovirus have been formulated and applied to suppress outbreaks of the gypsy moth, L. dispar. To evaluate the genetic diversity in this species at the genomic level, the genomes of three isolates from Massachusetts, USA (LdMNPV-Ab-a624), Spain (LdMNPV-3054), and Japan (LdMNPV-3041) were sequenced and compared with four previously determined LdMNPV genome sequences. The LdMNPV genome sequences were collinear and contained the same homologous repeats (hrs) and clusters of baculovirus repeat orf (bro) gene family members in the same relative positions in their genomes, although sequence identities in these regions were low. Of 146 non-bro ORFs annotated in the genome of the representative isolate LdMNPV 5-6, 135 ORFs were found in every other LdMNPV genome, including the 37 core genes of Baculoviridae and other genes conserved in genus Alphabaculovirus. Phylogenetic inference with an alignment of the core gene nucleotide sequences grouped isolates 3041 (Japan) and 2161 (Korea) separately from a cluster containing isolates from Europe, North America, and Russia. To examine phenotypic diversity, bioassays were carried out with a selection of isolates against neonate larvae from three European gypsy moth (Lymantria dispar dispar) and three Asian gypsy moth (Lymantria dispar asiatica and Lymantria dispar japonica) colonies. LdMNPV isolates 2161 (Korea), 3029 (Russia), and 3041 (Japan) exhibited a greater degree of pathogenicity against all L. dispar strains than LdMNPV from a sample of Gypchek. This study provides additional information on the genetic diversity of LdMNPV isolates and their activity against the Asian gypsy moth, a potential invasive pest of North American trees and forests.
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complete genome sequence of the strain of Lymantria dispar multiple nucleopolyhedrovirus found in the gypsy moth biopesticide virin ensh
Genome Announcements, 2015Co-Authors: Robert L Harrison, Daniel L RowleyAbstract:ABSTRACT We report the genome sequence of an alphabaculovirus from the gypsy moth (Lymantria dispar) biopesticide Virin-ENSh. The genome sequence is 161,712 bp, and its structure and sequence similarity indicate that the virus used in Virin-ENSh is a strain of the species Lymantria dispar multiple nucleopolyhedrovirus.
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classification genetic variation and pathogenicity of Lymantria dispar nucleopolyhedrovirus isolates from asia europe and north america
Journal of Invertebrate Pathology, 2014Co-Authors: Robert L Harrison, Melody A. Keena, Daniel L RowleyAbstract:Abstract Lymantria dispar multiple nucleopolyhedrovirus (LdMNPV) has been formulated and applied to control outbreaks of the gypsy moth, L. dispar. To classify and determine the degree of genetic variation among isolates of L. dispar NPVs from different parts of the range of the gypsy moth, partial sequences of the lef-8, lef-9, and polh genes were determined for Lymantria spp. virus samples from host populations throughout the world. Sequence analysis confirmed that all L. dispar virus samples tested contained isolates of the species Lymantria dispar multiple nucleopolyhedrovirus (Baculoviridae: Alphabaculovirus). Phylogenetic inference based on the lef-8 sequences indicated that the LdMNPV isolates formed two groups, one consisting primarily of isolates from Asia, and one consisting primarily of isolates from Europe and North America. The complete genome sequence was determined for an isolate from the Asian group, LdMNPV-2161 (S. Korea). The LdMNPV-2161 genome was 163,138 bp in length, 2092 bp larger than the previously determined genome of LdMNPV isolate 5–6 (CT, USA). The two genome sequences were co-linear, with an overall nucleotide sequence identity of 97.5% and some differences in ORF content. In droplet-feeding bioassays against neonate L. dispar larvae, isolates LdMNPV-3029 (Virin-ENSh/Russia) and LdMNPV-Ab-a624 (MA, USA) killed neonate larvae with an LC50 values that were 1.8- to 3.2-fold lower than a sample of Gypchek® (CT, USA) and isolates LdMNPV-3041 (Japan) and LdMNPV-2161. This study expands our knowledge about genetic variation among LdMNPV isolates and provides novel information on the distinct groups in which these NPVs occur.
Gernot Hoch - One of the best experts on this subject based on the ideXlab platform.
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Physiological effects of a microsporidian infection: alterations in carbohydrate and fatty acid levels in Lymantria dispar larvae
2020Co-Authors: Gernot Hoch, Christa Schafellner, Axel SchopfAbstract:Vairimorpha sp. is a virulent microsporidian pathogen isolated from Lymantria dispar L. in Central Europe.
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influence of the forest caterpillar hunter calosoma sycophanta on the transmission of microsporidia in larvae of the gypsy moth Lymantria dispar
Agricultural and Forest Entomology, 2013Co-Authors: Dorte Goertz, Gernot HochAbstract:1 The behaviour of predators can be an important factor in the transmission success of an insect pathogen. We studied how Calosoma sycophanta influences the interaction between its prey [Lymantria dispar (L.) (Lepidoptera, Lymantriidae)] and two microsporidian pathogens [Nosema Lymantriae (Microsporidia, Nosematidae) and Vairimorpha disparis (Microsporidia, Burellenidae)] infecting the prey. 2 Using laboratory experiments, C. sycophanta was allowed to forage on infected and uninfected L. dispar larvae and to disseminate microsporidian spores when preying or afterwards with faeces. 3 The beetle disseminated spores of N. Lymantriae and V. disparis when preying upon infected larvae, as well as after feeding on such prey. Between 45% and 69% of test larvae became infected when C. sycophanta was allowed to disseminate spores of either microsporidium. 4 Laboratory choice experiments showed that C. sycophanta did not discriminate between Nosema-infected and uninfected gypsy moth larvae. Calosoma sycophanta preferred Vairimorpha-infected over uninfected gypsy moth larvae and significantly influenced transmission. 5 When C. sycophanta was allowed to forage during the latent period on infected and uninfected larvae reared together on caged, potted oak saplings, the percentage of V. disparis infection among test larvae increased by more than 70%. The transmission of N. Lymantriae was not affected significantly in these experiments. 6 Beetles never became infected with either microsporidian species after feeding on infected prey. 7 We conclude that the transmission of N. Lymantriae is not affected. Because no V. disparis spores are released from living larvae, feeding on infected larvae might enhance transmission by reducing the time to death and therefore the latent period.
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quantifying horizontal transmission of nosema Lymantriae a microsporidian pathogen of the gypsy moth Lymantria dispar lep lymantriidae in field cage studies
Journal of Invertebrate Pathology, 2008Co-Authors: Gernot Hoch, Vincent Damico, Leellen F Solter, Milan Zubrik, Michael L McmanusAbstract:Abstract Nosema Lymantriae is a microsporidian pathogen of the gypsy moth, Lymantria dispar that has been documented to be at least partially responsible for the collapse of L. dispar outbreak populations in Europe. To quantify horizontal transmission of this pathogen under field conditions we performed caged-tree experiments that varied (1) the density of the pathogen through the introduction of laboratory-infected larvae, and (2) the total time that susceptible (test) larvae were exposed to these infected larvae. The time frame of the experiments extended from the early phase of colonization of the target tissues by the microsporidium to the onset of pathogen-induced mortality or pupation of test larvae. Upon termination of each experiment, the prevalence of infection in test larvae was evaluated. In the experiments performed over a range of pathogen densities, infection of test larvae increased with increasing density of inoculated larvae, from 14.2 ± 3.5% at density of 10 inoculated per 100 larvae to 36.7 ± 5.7% at 30 inoculated per 100 larvae. At higher densities, percent infection in test larvae appeared to level off (35.7 ± 5.5% at 50 inoculated per 100 larvae). When larval exposure to the pathogen was varied, transmission of N. Lymantriae did not occur within the first 15 d post-inoculation (dpi) (11 d post-exposure of test larvae to inoculated larvae). We found the first infected test larvae in samples taken 20 dpi (16 d post-exposure). Transmission increased over time; in the cages sampled 25 dpi (21 d post-exposure), Nosema prevalence in test larvae ranged from 20.6% to 39.2%.
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horizontal transmission pathways of terrestrial microsporidia a quantitative comparison of three pathogens infecting different organs in Lymantria dispar l lep lymantriidae larvae
Biological Control, 2008Co-Authors: Dorte Goertz, Gernot HochAbstract:Abstract Pathways for horizontal transmission of three microsporidian species, such as transmission via silk, feces, cadaver, exuviae and direct contact, were studied in Lymantria dispar larvae. The midgut infecting Endoreticulatus schubergi caused low larval mortality; 60% of infected L. dispar survived until the adult stage. An infected larva released on average 1.6 × 108 spores with feces until pupation, indicating that this is the main route for transmission. The more virulent fat body parasite, Vairimorpha disparis, caused the early death of infected L. dispar larvae. The high spore load of the cadaver (4.6 × 109 spores/cadaver), which was independent of the inoculation dosage, indicates that the release of spores from decomposing cadavers is the main transmission pathway. The third studied microsporidium, Nosema Lymantriae, caused a systemic infection and killed the host mostly in the larval stage (>90%). Spores were released from living hosts with feces (2.7 × 108 spores in total), and later from decomposing cadavers (4.9 × 109 spores/cadaver on average). Thus, we assume that both pathways are important for this species. Susceptible larvae contracted an infection with N. Lymantriae or E. schubergi when they came into contact with either infected larvae or their exuviae.
Ren Iwaizumi - One of the best experts on this subject based on the ideXlab platform.
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Identification of Japanese Lymantria species (Lepidoptera: Lymantriidae) based on DNA sequences
Applied Entomology and Zoology, 2018Co-Authors: Makoto Arimoto, Ren IwaizumiAbstract:We examined the utility of the cytochrome c oxidase subunit I (COI) and II (COII) genes of mitochondrial DNA (mtDNA) as a tool to identify nine Japanese Lymantria species, including four Asian gypsy moth species [Lymantria dispar japonica (Motschulsky), Lymantria umbrosa (Butler), Lymantria albescens Hori and Umeno, and Lymantria postalba Inoue]. In phylogenetic trees constructed for the COI and COII genes using the maximum likelihood methods, we could identify seven out of the nine Lymantria species (L. albescens and L. postalba could not be identified). These results suggest that the DNA sequences of the COI and COII genes are useful for identifying Japanese Lymantria species.
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PCR-RFLP analysis of the ITS2 region to identify Japanese Lymantria species (Lepidoptera: Lymantriidae)
Applied Entomology and Zoology, 2015Co-Authors: Makoto Arimoto, Ren IwaizumiAbstract:In this study, a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)-based method for species identification was applied to eight Japanese Lymantria species, including the four “Asian gypsy moth” species [Lymantria dispar japonica (Motschulsky), Lymantria umbrosa (Butler), Lymantria albescens Hori and Umeno, and Lymantria postalba Inoue] and four other species [Lymantria xylina Swinhoe, Lymantria mathura Moore, Lymantria monacha (Linnaeus), and Lymantria lucescens (Butler)]. We sequenced the second internal transcribed spacer (ITS2) region of nuclear ribosomal DNA (rDNA) of one individual from each of the eight species. We analyzed the recognition sites of a restriction endonuclease (MnlI) to identify the Lymantria species by PCR-RFLP. We then applied PCR-RFLP analysis to 330 individuals from 54 populations of the 8 species. As a result, these species could be correctly identified with the exception of two species pairs (L. dispar japonica and L. umbrosa; L. albescens and L. postalba). These results suggest that this PCR-RFLP method based on the ITS2 region is useful for identifying Japanese Lymantria species.
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identification of japanese Lymantria species lepidoptera lymantriidae based on pcr rflp analysis of mitochondrial dna
Applied Entomology and Zoology, 2014Co-Authors: Makoto Arimoto, Ren IwaizumiAbstract:A polymerase chain reaction-restriction fragment length polymorphism (PCR–RFLP)-based method for species identification was applied to seven Japanese Lymantria species, including four Asian gypsy moth (AGM) species. We sequenced the partial end of the cytochrome c oxidase I (COI) gene, tRNA leucine, COII gene, and partial end of the tRNA lysine in mitochondrial DNA (mtDNA) for one individual of each of the seven species. We analyzed the recognition sites of three restriction endonucleases and constructed a scheme for Lymantria species identification using PCR–RFLP. We then applied the scheme to 291 individuals from 45 populations of seven species. We found that all seven species were correctly identified using PCR–RFLP. These results suggest that PCR–RFLP is useful for identifying Japanese Lymantria species, which may be detected at Japanese ports.
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Identification of Japanese Lymantria species (Lepidoptera: Lymantriidae) based on PCR–RFLP analysis of mitochondrial DNA
Applied Entomology and Zoology, 2013Co-Authors: Makoto Arimoto, Ren IwaizumiAbstract:A polymerase chain reaction-restriction fragment length polymorphism (PCR–RFLP)-based method for species identification was applied to seven Japanese Lymantria species, including four Asian gypsy moth (AGM) species. We sequenced the partial end of the cytochrome c oxidase I (COI) gene, tRNA leucine, COII gene, and partial end of the tRNA lysine in mitochondrial DNA (mtDNA) for one individual of each of the seven species. We analyzed the recognition sites of three restriction endonucleases and constructed a scheme for Lymantria species identification using PCR–RFLP. We then applied the scheme to 291 individuals from 45 populations of seven species. We found that all seven species were correctly identified using PCR–RFLP. These results suggest that PCR–RFLP is useful for identifying Japanese Lymantria species, which may be detected at Japanese ports.
