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Y Li - One of the best experts on this subject based on the ideXlab platform.

  • pharmacokinetic study of Mangiferin in human plasma after oral administration
    Food Chemistry, 2012
    Co-Authors: Fang Wang, Y Li, Maoqing Wang
    Abstract:

    Abstract Mangiferin, an active component of traditional Chinese herbal medicine, although it is reported to have various pharmacological effects, the limited number of pharmacokinetic studies limit its wide application. To evaluate the pharmacokinetics of Mangiferin in human, a sensitive high performance liquid chromatography–mass spectrometry (HPLC–MS) method for the determination of Mangiferin in human plasma was developed. The proposed HPLC–MS method is selective, precise and accurate enough and enables the identification and quantification of Mangiferin for the use in clinical studies. After single oral administration of 0.1, 0.3 and 0.9 g Mangiferin, respectively, the method was successfully applied for the pharmacokinetics of Mangiferin in 21 healthy male Chinese volunteers. The pharmacokinetic of Mangiferin was fit to the non-compartmental model. The pharmacokinetics parameters were calculated. Mangiferin concentration in plasma reached 38.64 ± 6.75 ng/mL about 1 h after oral administration of 0.9 g Mangiferin and the the apparent elimination half-life ( t 1/2 ) was 7.85 ± 1.72 h. The absorption of Mangiferin was increased with the administration of a large dose and it was concluded that the pharmacokinetics of Mangiferin in human was nonlinear.

  • Mangiferin decreases plasma free fatty acids through promoting its catabolism in liver by activation of ampk
    PLOS ONE, 2012
    Co-Authors: Songtao Li, Lixin Na, Rennan Feng, Y Li
    Abstract:

    Mangiferin has been shown to have the effect of improving dyslipidemia. Plasma free fatty acids (FFA) are closely associated with blood lipid metabolism as well as many diseases including metabolic syndrome. This study is to investigate whether Mangiferin has effects on FFA metabolism in hyperlipidemic rats. Wistar rats were fed a high-fat diet and administered Mangiferin simultaneously for 6 weeks. Mangiferin (50, 100, 150 mg/kg BW) decreased dose-dependently FFA and triglycerides (TG) levels in plasma, and their accumulations in liver, but increased the β-hydroxybutyrate levels in both plasma and liver of hyperlipidemic rats. HepG2 cells were treated with oleic acid (OA, 0.2 mmol/L) to simulate the condition of high level of plasma FFA in vitro, and were treated with different concentrations of Mangiferin simultaneously for 24 h. We found that Mangiferin significantly increased FFA uptake, significantly decreased intracellular FFA and TG accumulations in HepG2 cells. Mangiferin significantly increased AMP-activated protein kinase (AMPK) phosphorylation and its downstream proteins involved in fatty acid translocase (CD36) and carnitine palmitoyltransferase 1 (CPT1), but significantly decreased acyl-CoA: diacylgycerol acyltransferase 2 (DGAT2) expression and acetyl-CoA carboxylase (ACC) activity by increasing its phosphorylation level in both in vivo and in vitro studies. Furthermore, these effects were reversed by Compound C, an AMPK inhibitor in HepG2 cells. For upstream of AMPK, Mangiferin increased AMP/ATP ratio, but had no effect on LKB1 phosphorylation. In conclusion, Mangiferin decreased plasma FFA levels through promoting FFA uptake and oxidation, inhibiting FFA and TG accumulations by regulating the key enzymes expression in liver through AMPK pathway. Therefore, Mangiferin is a possible beneficial natural compound for metabolic syndrome by improving FFA metabolism.

  • beneficial effects of Mangiferin on hyperlipidemia in high fat fed hamsters
    Molecular Nutrition & Food Research, 2011
    Co-Authors: Conghui Huang, Rennan Feng, Xilu Liao, Yemei Wang, Ying He, Y Li
    Abstract:

    Scope: Mangiferin, a natural polyphenol, has been shown to have hypolipidemic effect in rat and mouse. However, the mechanism of action is not well understood. This study was conducted to determine the effect and mechanism of action of Mangiferin on hyperlipidemia induced in hamsters by a high-fat diet. Methods and results: Forty male hamsters were randomly assigned to normal control, high-fat control, and high fat with Mangiferin (50 and 150 mg/kg BW) groups. Mangiferin treatment significantly decreased final body weight, liver weight and visceral fat-pad weight, serum triglyceride (TG) and total free fatty acid (FFA) concentrations, hepatic TG levels and hepatic and muscle total FFA contents. Mangiferin upregulated mRNA expression of peroxisome proliferator-activated receptor-α (PPAR-α), fatty acid translocase (CD36) and carnitine palmitoyltransferase 1 (CPT-1), but downregulated mRNA expression of sterol regulatory element-binding protein 1c (SREBP-1c), acetyl CoA carboxylase (ACC), acyl-CoA:diacylglycerol acyltransferase 2 (DGAT-2) and microsomal triglyceride transfer protein (MTP) in liver. Mangiferin also stimulated mRNA expression of PPAR-α, CD36, CPT-1 and lipoprotein lipase (LPL) in muscle. Conclusions: The results suggest that Mangiferin may ameliorate hypertriglyceridemia partly by modulating the expression levels of genes involved in lipid oxidation and lipogenesis.

Chenggang Huang - One of the best experts on this subject based on the ideXlab platform.

  • structure elucidation of in vivo and in vitro metabolites of Mangiferin
    Journal of Pharmaceutical and Biomedical Analysis, 2011
    Co-Authors: Ke Wang, Yihong Tang, Longhai Jian, Zhixiong Li, Bin Wu, Chenggang Huang
    Abstract:

    Abstract The in vivo and in vitro metabolism of Mangiferin was systematically investigated. Urine, plasma, feces, contents of intestinal tract and various organs were collected after oral administration of Mangiferin to healthy rats at a dose of 200 mg/kg body weight. For comparison, Mangiferin was also incubated in vitro with intestinal flora of rats. With the aid of a specific and sensitive liquid chromatography coupled with electrospray ionization tandem hybrid ion trap mass spectrometry (LC–ESI–IT–MS n ), a total of thirty-three metabolites of Mangiferin were detected and their structures were tentatively elucidated on the basis of the characteristics of their precursor ions, product ions and chromatographic retention times. The biotransformation pathways of Mangiferin involved deglycosylation, dehydroxylation, methylation, glycosylation, glucuronidation and sulfation.

  • high performance liquid chromatographic method for the determination of Mangiferin in rat plasma and urine
    Biomedical Chromatography, 2006
    Co-Authors: Hui Wang, Yihong Tang, Guan Ye, Chenggang Huang
    Abstract:

    A reversed-phase high-performance liquid chromatography assay for Mangiferin in rat plasma and urine was developed. Rutin was employed as an internal standard. The mobile phase consisted of acetonitrile–water (16:84, v/v) containing 3% acetic acid at a flow rate of 1 mL/min. Detection was at 257 and 365 nm for Mangiferin in plasma and urine, respectively. The limit of quantitation (LOQ) of Mangiferin was 0.6 mg/mL in plasma, and 0.48 mg/mL in urine. The standard curve was linear from 0.6 to 24 mg/mL in plasma, and 0.48 to 24 mg/mL in urine, both intra- and inter-day precision of the Mangiferin were determined and their RSD did not exceed 10%. The method provides a technique for rapid analysis of Mangiferin in rat plasma and urine, which can be used in pharmacokinetic studies. Copyright © 2006 John Wiley & Sons, Ltd.

Shri Hari Mishra - One of the best experts on this subject based on the ideXlab platform.

  • Rapid validated high performance thin layer chromatography method for simultaneous estimation of Mangiferin and scopoletin in Canscora decussata (South Indian Shankhpushpi) extract
    Revista Brasileira de Farmacognosia, 2015
    Co-Authors: Neeraj K. Sethiya, Ashish Trivedi, Shri Hari Mishra
    Abstract:

    Mangiferin (polyphenolic xanthone) and scopoletin (phenolic coumarin) are well-studied biological markers present in Canscora decussata (Roxb.) Roem. & Schult., Gentianaceae. The objective set for the present studies is to establish and develop a new, simple, selective, sensitive, and precise high performance thin layer chromatography method for the simultaneous estimation of Mangiferin and scopoletin in hydroalcoholic extract of C. decussata . The thin layer chromatographic separation of these biomarkers was carried out on aluminum plate pre-coated with silica gel 60F_254, eluted with ethyl acetate:acetic:acid:formic acid:water (10:0.5:0.5:1.5). The plate was then dried and densitometric scanning was performed at 254 nm using a Camag TLC scanner III. The system was found to give compact spots for Mangiferin ( R _F0.22) and scopoletin ( R _F0.78). A good relationship of linear precision between the concentrations (100–600 ng/spot) and peak areas was obtained with correlation coefficient ( r ) of 0.9979 (Mangiferin) and 0.9962 (scopoletin), respectively. The limits of detection and limit of quantification were determined to be 46 and 94 ng/spot for Mangiferin and 31 and 78 ng/spot for scopoletin respectively. The percentage of recovery was found from 99.91 to 99.94% for Mangiferin and 99.75 to 99.86% for scopoletin. Results obtained from recovery studies showed excellent reliability and reproducibility of the method. Present communication on validated high performance thin layer chromatography method may provide a new, selective, sensitive, and precise method to estimate Mangiferin and scopoletin as phytomarkers in the hydroalcoholic extract of C. decussata used in Ayurvedic formulations.© 2015 Sociedade Brasileira de Farmacognosia. Published by Elsevier Editora Ltda. All rights reserved.

  • rapid validated high performance thin layer chromatography method for simultaneous estimation of Mangiferin and scopoletin in canscora decussata south indian shankhpushpi extract
    Revista Brasileira De Farmacognosia-brazilian Journal of Pharmacognosy, 2015
    Co-Authors: Neeraj K. Sethiya, Ashish Trivedi, Shri Hari Mishra
    Abstract:

    Mangiferin (polyphenolic xanthone) and scopoletin (phenolic coumarin) are well-studied biological markers present in Canscora decussata(Roxb.) Roem. & Schult., Gentianaceae. The objective set for the present studies is to establish and develop a new, simple, selective, sensitive, and precise high performance thin layer chromatography method for the simultaneous estimation of Mangiferin and scopoletin in hydroalcoholic extract of C. decussata. The thin layer chromatographic separation of these biomarkers was carried out on aluminum plate pre-coated with silica gel 60F254, eluted with ethyl acetate:acetic acid:formic acid:water (10:0.5:0.5:1.5). The plate was then dried and densitometric scanning was performed at 254 nm using a Camag TLC scanner III. The system was found to give compact spots for Mangiferin (RF 0.22) and scopoletin (RF 0.78). A good relationship of linear precision between the concentrations (100–600 ng/spot) and peak areas was obtained with correlation coefficient (r) of 0.9979 (Mangiferin) and 0.9962 (scopoletin), respectively. The limits of detection and limit of quantification were determined to be 46 and 94 ng/spot for Mangiferin and 31 and 78 ng/spot for scopoletin respectively. The percentage of recovery was found from 99.91 to 99.94% for Mangiferin and 99.75 to 99.86% for scopoletin. Results obtained from recovery studies showed excellent reliability and reproducibility of the method. Present communication on validated high performance thin layer chromatography method may provide a new, selective, sensitive, and precise method to estimate Mangiferin and scopoletin as phytomarkers in the hydroalcoholic extract of C. decussata used in Ayurvedic formulations.

Wang Hanmin - One of the best experts on this subject based on the ideXlab platform.

  • protective effects of Mangiferin on cerebral ischemia reperfusion injury and its mechanisms
    European Journal of Pharmacology, 2016
    Co-Authors: Zhang Yang, Chen Weian, Huang Susu, Wang Hanmin
    Abstract:

    Abstract The aim of our study was to investigate the protective properties of Mangiferin, a natural glucosyl xanthone found in both mango and papaya on the cerebral ischemia–reperfusion injury and the underlying mechanism. Wistar male rats were subjected to middle cerebral artery occlusion for 2 h followed by 24 h of reperfusion. Mangiferin (25, 50, and 100 mg/kg, ig) or 0.5% carboxymethyl cellulose sodium was administered three times before ischemia and once at 2 h after the onset of ischemia. Neurological score, infarct volume, and brain water content, some oxidative stress markers and inflammatory cytokines were evaluated after 24 h of reperfusion. Treatment with Mangiferin significantly ameliorated neurologic deficit, infarct volume and brain water content after cerebral ischemia reperfusion. Mangiferin also reduced the content of malondialdehyde (MDA), IL-1β and TNF-α, and up-regulated the activities of superoxide dismutase (SOD), glutathione (GSH) and IL-10 levels in the brain tissue of rats with the cerebral ischemia–reperfusion injury. Moreover, Mangiferin up-regulated the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream anti-oxidant protein heme oxygenase-1 (HO-1). The results indicate that Mangiferin can play a certain protective role in the cerebral ischemia–reperfusion injury, and the protective effect of Mangiferin may be related to the improvement on the antioxidant capacity of brain tissue and the inhibition of overproduction of inflammatory cytokines. The mechanisms are associated with enhancing the oxidant defense systems via the activation of Nrf2/HO-1 pathway.

Dharamvir Singh Arya - One of the best experts on this subject based on the ideXlab platform.

  • protective effect of Mangiferin on myocardial ischemia reperfusion injury in streptozotocin induced diabetic rats role of age rage mapk pathways
    Scientific Reports, 2017
    Co-Authors: Kapil Suchal, Salma Malik, Sana Irfan Khan, Rajiv Kumar Malhotra, Sameer N Goyal, Jagriti Bhatia, Santosh Kumari, Shreesh Ojha, Dharamvir Singh Arya
    Abstract:

    Hyperglycemia induced advanced glycation end products-receptor for advanced glycation end products (AGE-RAGE) activation is thought to involve in the development of cardiovascular disease in diabetics. Activation of AGE-RAGE axis results in the oxidative stress and inflammation. Mangiferin is found in the bark of mango tree and is known to treat diseases owing to its various biological activities. Thus, this study was designed to evaluate the effect of Mangiferin in ischemia-reperfusion (IR) induced myocardial injury in diabetic rats. A single injection of STZ (70 mg/kg; i.p.) was injected to male albino Wistar rats to induce diabetes. After confirmation of diabetes, rats were administered vehicle (2 ml/kg; i.p.) and Mangiferin (40 mg/kg; i.p.) for 28 days. On 28th day, left anterior descending coronary artery was ligated for 45 min and then reperfused for 60 min. Mangiferin treatment significantly improved cardiac function, restored antioxidant status, reduced inflammation, apoptosis and maintained myocardial architecture. Furthermore, Mangiferin significantly inhibited the activation of AGE-RAGE axis, c-Jun N-terminal kinase (JNK) and p38 and increased the expression of extracellular regulated kinase 1/2 (ERK1/2) in the myocardium. Thus, Mangiferin attenuated IR injury in diabetic rats by modulation of AGE-RAGE/MAPK pathways which further prevented oxidative stress, inflammation and apoptosis in the myocardium.