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Miguel Vences - One of the best experts on this subject based on the ideXlab platform.
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Mitochondrial Introgression, Color Pattern Variation, and Severe Demographic Bottlenecks in Three Species of Malagasy Poison Frogs, Genus Mantella
'MDPI AG', 2019Co-Authors: Angelica Crottini, Falitiana C E Rabemananjara, Pablo Orozco-terwengel, Susanne J. Hauswaldt, Miguel VencesAbstract:Madagascar is a biodiversity hotspot particularly rich in amphibian diversity and only a few charismatic Malagasy amphibians have been investigated for their population-level differentiation. The Mantella madagascariensis group is composed of two rainforest and three swamp forest species of poison frogs. We first confirm the monophyly of this clade using DNA sequences of three nuclear and four mitochondrial genes, and subsequently investigate the population genetic differentiation and demography of the swamp forest species using one mitochondrial, two nuclear and a set of nine microsatellite markers. Our results confirm the occurrence of two main mitochondrial lineages, one dominated by Mantella aurantiaca (a grouping supported also by our microsatellite-based tree) and the other by Mantella crocea + Mantella milotympanum. These two main lineages probably reflect an older divergence in swamp Mantella. Widespread mitochondrial introgression suggests a fairly common occurrence of inter-lineage gene flow. However, nuclear admixture seems to play only a limited role in this group, and the analyses of the RAG-1 marker points to a predominant incomplete lineage sorting scenario between all five species of the group, which probably diverged relatively recently. Our demographic analyses show a common, severe and recent demographic contraction, inferred to be in temporal coincidence with the massive deforestation events that took place in the past 1000 years. Current data do not allow to conclusively delimit independent evolutionary units in these frogs, and we therefore refrain to suggest any taxonomic changes
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RABEMANANJARA,
2016Co-Authors: David R. Vieites, Miguel Vences, Franco Andreone, Sandra Nieto-román, Axel MeyerAbstract:Blackwell Publishing LtdMitochondrial evidence for distinct phylogeographic units in the endangered Malagasy poison frog Mantella bernhard
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2002): Molecular phylogeny of Malagasy poison frogs, genus Mantella (Anura: Mantellidae): homoplastic evolution of colour pattern in aposematic amphibians. – Organisms Diversity and Evolution
2015Co-Authors: Hans-christian Schaefer, Miguel Vences, Michael VeithAbstract:We studied the evolution of colour pattern in Malagasy poison frogs, genus Mantella, a group of diurnal and toxic frogs endemic to Madagas-car. Based on a phylogeny reconstructed using 1130 bp of the mitochondrial 16S rRNA gene, the genus can be divided into five species groups. Within some of these groups, interspecific genetic divergences were very low (1.2–2.8 % sequence divergence) while colour patterns were markedly different. In contrast, Mantella madagascariensis and M. baroni, two species which show extremely similar dorsal coloration patterns, were not included in the same clade. This conclusion was supported by high bootstrap values and by significant rejection of alternative topolo-gies using KH-tests.Analysis of colour patterns and tentative reconstruction of ancestral states yielded five character states shared by these two species but not by their respective sister species, M. aurantiaca and M. nigricans. Considering these detailed similarities as symplesiomorphic therefore requires the assumption of multiple reversals in other species, whereas a homoplastic colour evolution in the sympatric M. madagas-cariensis and M. baroni appears as most parsimonious. This parallelism may have been triggered by Müllerian mimicry. However, additional data is necessary to support this hypothesis
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Individual and Geographic Variation of Skin Alkaloids in Three Swamp-Forest Species of Madagascan Poison Frogs (Mantella)
Journal of Chemical Ecology, 2015Co-Authors: Nirina R. Andriamaharavo, Miguel Vences, H. Martin Garraffo, Thomas F. Spande, Lesley-ann Giddings, David R. Vieites, Ralph A. SaporitoAbstract:Seventy skins of three mantellid frog species from Madagascan swamp-forest habitats, Mantella aurantiaca , M. crocea , and M. milotympanum, were individually examined for skin alkaloids using GC/MS. These poison frogs were found to differ significantly in their alkaloid composition from species of Mantella originating from non-flooded rainforest in eastern Madagascar, which were examined in earlier work. Only 16 of the previously detected 106 alkaloids were represented among the 60 alkaloids from the swamp-forest frogs of the present study. We hypothesize this difference is related mainly to habitat but cannot exclude a phylogenetic component as the three swamp-forest species are a closely related monophyletic group. The paucity of alkaloids with unbranched-carbon skeletons (ant-derived) and the commonness of alkaloids with branched-carbon skeletons (mite-derived) indicate that oribatid mites are a major source of alkaloids in these species of mantellids. Furthermore, most of the alkaloids have an oxygen atom in their formulae. Differences in alkaloids were observed among species, populations of the same species, and habitats. In M. aurantiaca , small geographic distances among populations were associated with differences in alkaloid profiles, with a remote third site illustrating even greater differences. The present study and an earlier study of three other mantellid species suggest that oribatid mites, and not ants, are the major source of alkaloids in the species of mantellids examined thus far.
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Chromosome Data for Malagasy Poison Frogs (Amphibia: Ranidae: Mantella) and Their Bearing on Taxonomy and Phylogeny
2014Co-Authors: Gaetano Odierna, Miguel Vences, Gennaro Aprea, Stefan Lötters, Franco AndreoneAbstract:ABSTRACT—We compared chromosome morphologies for 11 species of Malagasy poison frogs, genus Mantella, and three outgroup taxa (genus Mantidactylus) using conventional and fluorescence staining tech-niques. All species studied had a karyotype of 2n=26, with five larger and eight smaller chromosome pairs. The 11th pair was acrocentic in Mantella nigricans which represents the first such observation in the genus. The nucleolus organizer region (NOR) was located at secondary constrictions on chromosome pair 2 in all Mantella studied and in Mantidactylus grandisonae (while located on other chromosomes in all other species of Mantidactylus studied so far). Heterochromatin distribution was highly variable among Mantella species; C-bands positively staining with DAPI and CMA3 were observed. The possible structure of these bands, seemingly containing both A+T rich and C+G rich heterochromatin, is discussed. Phylogenetic reconstruc-tion using chromosomal characters provided very little information. Evolution of the characters studied is probably either too fast (heterochromatin arrangement) or too slow (NOR location) to match the main cladogenetic events among Mantella species groups
Falitiana C E Rabemananjara - One of the best experts on this subject based on the ideXlab platform.
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Mitochondrial Introgression, Color Pattern Variation, and Severe Demographic Bottlenecks in Three Species of Malagasy Poison Frogs, Genus Mantella
'MDPI AG', 2019Co-Authors: Angelica Crottini, Falitiana C E Rabemananjara, Pablo Orozco-terwengel, Susanne J. Hauswaldt, Miguel VencesAbstract:Madagascar is a biodiversity hotspot particularly rich in amphibian diversity and only a few charismatic Malagasy amphibians have been investigated for their population-level differentiation. The Mantella madagascariensis group is composed of two rainforest and three swamp forest species of poison frogs. We first confirm the monophyly of this clade using DNA sequences of three nuclear and four mitochondrial genes, and subsequently investigate the population genetic differentiation and demography of the swamp forest species using one mitochondrial, two nuclear and a set of nine microsatellite markers. Our results confirm the occurrence of two main mitochondrial lineages, one dominated by Mantella aurantiaca (a grouping supported also by our microsatellite-based tree) and the other by Mantella crocea + Mantella milotympanum. These two main lineages probably reflect an older divergence in swamp Mantella. Widespread mitochondrial introgression suggests a fairly common occurrence of inter-lineage gene flow. However, nuclear admixture seems to play only a limited role in this group, and the analyses of the RAG-1 marker points to a predominant incomplete lineage sorting scenario between all five species of the group, which probably diverged relatively recently. Our demographic analyses show a common, severe and recent demographic contraction, inferred to be in temporal coincidence with the massive deforestation events that took place in the past 1000 years. Current data do not allow to conclusively delimit independent evolutionary units in these frogs, and we therefore refrain to suggest any taxonomic changes
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evidence for recent gene flow between north eastern and south eastern madagascan poison frogs from a phylogeography of the Mantella cowani group
Frontiers in Zoology, 2007Co-Authors: Falitiana C E Rabemananjara, Olga Ramilijaona, Ylenia Chiari, Miguel VencesAbstract:Background The genus Mantella, endemic poison frogs of Madagascar with 16 described species, are known in the field of international pet trade and entered under the CITES control for the last four years. The phylogeny and phylogeography of this genus have been recently subject of study for conservation purposes. Here we report on the studies of the phylogeography of the Mantella cowani group using a fragment of 453 bp of the mitochondrial cytochrome b gene from 195 individuals from 21 localities. This group is represented by five forms: M. cowani, a critically endangered species, a vulnerable species, M. haraldmeieri, and the non-threatened M. baroni, M. aff. baroni, and M. nigricans.
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evidence for recent gene flow between north eastern and south eastern madagascan poison frogs from a phylogeography of the Mantella cowani group
Frontiers in Zoology, 2007Co-Authors: Falitiana C E Rabemananjara, Olga Ramilijaona, Ylenia Chiari, Miguel VencesAbstract:The genus Mantella, endemic poison frogs of Madagascar with 16 described species, are known in the field of international pet trade and entered under the CITES control for the last four years. The phylogeny and phylogeography of this genus have been recently subject of study for conservation purposes. Here we report on the studies of the phylogeography of the Mantella cowani group using a fragment of 453 bp of the mitochondrial cytochrome b gene from 195 individuals from 21 localities. This group is represented by five forms: M. cowani, a critically endangered species, a vulnerable species, M. haraldmeieri, and the non-threatened M. baroni, M. aff. baroni, and M. nigricans. The Bayesian phylogenetic and haplotype network analyses revealed the presence of three separated haplotype clades: (1) M. baroni, M. aff. baroni, M. nigricans, and putative hybrids of M. cowani and M. baroni, (2) M. cowani and putative hybrids of M. cowani and M. baroni, and (3) M. haraldmeieri. The putative hybrids were collected from sites where M. cowani and M. baroni live in sympatry. These results suggest (a) a probable hybridization between M. cowani and M. baroni, (b) a lack of genetic differentiation between M. baroni/M. aff. baroni and M. nigricans, (c) evidence of recent gene-flow between the northern (M. nigricans), eastern (M. baroni), and south-eastern (M. aff. baroni) forms of distinct coloration, and (d) the existence of at least three units for conservation in the Mantella cowani group.
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Evidence for recent gene flow between north-eastern and south-eastern Madagascan poison frogs from a phylogeography of the Mantella cowani group
Frontiers in Zoology, 2007Co-Authors: Falitiana C E Rabemananjara, Olga Ramilijaona, Ylenia Chiari, Miguel VencesAbstract:Background The genus Mantella , endemic poison frogs of Madagascar with 16 described species, are known in the field of international pet trade and entered under the CITES control for the last four years. The phylogeny and phylogeography of this genus have been recently subject of study for conservation purposes. Here we report on the studies of the phylogeography of the Mantella cowani group using a fragment of 453 bp of the mitochondrial cytochrome b gene from 195 individuals from 21 localities. This group is represented by five forms: M. cowani , a critically endangered species, a vulnerable species, M. haraldmeieri , and the non-threatened M. baroni, M . aff. baroni , and M. nigricans . Results The Bayesian phylogenetic and haplotype network analyses revealed the presence of three separated haplotype clades: (1) M. baroni, M . aff. baroni, M. nigricans , and putative hybrids of M. cowani and M. baroni , (2) M. cowani and putative hybrids of M. cowani and M. baroni , and (3) M. haraldmeieri . The putative hybrids were collected from sites where M. cowani and M. baroni live in sympatry. Conclusion These results suggest (a) a probable hybridization between M. cowani and M. baroni , (b) a lack of genetic differentiation between M. baroni/M . aff. baroni and M. nigricans , (c) evidence of recent gene-flow between the northern ( M. nigricans ), eastern ( M. baroni ), and south-eastern ( M . aff. baroni ) forms of distinct coloration, and (d) the existence of at least three units for conservation in the Mantella cowani group.
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Molecular systematics of Malagasy poison frogs in the Mantella betsileo and M. laevigata species groups
'Pharmapress LLC', 2007Co-Authors: Falitiana C E Rabemananjara, Y. Chiari, P. Bora, A. Crottini, F. Andreone, F. Glaw, R. Duguet, Ravoahangimalala O. Ramilijaona, Miguel VencesAbstract:Malagasy poison frogs of the genus Mantella with its 16 species are currently sub-divided into 5 major groups. Of these, the Mantella betsileo group is traditionally understood as containing four species, Mantella betsileo, M. expectata, M. viridis and M. manery, while the M. laevigata group is considered to be monospecific. A phylogenetic analysis of samples from multiple localities of all species in these two groups, based on sequences of the mitochondrial cytochrome b gene, shows the existence of several well-distinct clades in what is currently considered to be Mantella betsileo: (1) central-western populations from Kirindy, Isalo, and near Antsirabe close to the Betsileo region, to which the name M. betsileo is to be applied, (2) populations of the north-east and north-west, which are closely related to M. viridis and to which the name M. ebenaui is to be applied, and (3) a clade from southernmost Madagascar and from the Tsingy de Bemaraha, which is sister to M. expectata and furthermore includes important intra-clade variation, therefore probably representing one or two undescribed species. Our data also support a large genetic distance of M. manery to all other species and its probable sister-group relationship to the sympatric M. laevigata; M. manery is consequently transferred from the M. betsileo group to the M. laevigata group
Ylenia Chiari - One of the best experts on this subject based on the ideXlab platform.
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evidence for recent gene flow between north eastern and south eastern madagascan poison frogs from a phylogeography of the Mantella cowani group
Frontiers in Zoology, 2007Co-Authors: Falitiana C E Rabemananjara, Olga Ramilijaona, Ylenia Chiari, Miguel VencesAbstract:Background The genus Mantella, endemic poison frogs of Madagascar with 16 described species, are known in the field of international pet trade and entered under the CITES control for the last four years. The phylogeny and phylogeography of this genus have been recently subject of study for conservation purposes. Here we report on the studies of the phylogeography of the Mantella cowani group using a fragment of 453 bp of the mitochondrial cytochrome b gene from 195 individuals from 21 localities. This group is represented by five forms: M. cowani, a critically endangered species, a vulnerable species, M. haraldmeieri, and the non-threatened M. baroni, M. aff. baroni, and M. nigricans.
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evidence for recent gene flow between north eastern and south eastern madagascan poison frogs from a phylogeography of the Mantella cowani group
Frontiers in Zoology, 2007Co-Authors: Falitiana C E Rabemananjara, Olga Ramilijaona, Ylenia Chiari, Miguel VencesAbstract:The genus Mantella, endemic poison frogs of Madagascar with 16 described species, are known in the field of international pet trade and entered under the CITES control for the last four years. The phylogeny and phylogeography of this genus have been recently subject of study for conservation purposes. Here we report on the studies of the phylogeography of the Mantella cowani group using a fragment of 453 bp of the mitochondrial cytochrome b gene from 195 individuals from 21 localities. This group is represented by five forms: M. cowani, a critically endangered species, a vulnerable species, M. haraldmeieri, and the non-threatened M. baroni, M. aff. baroni, and M. nigricans. The Bayesian phylogenetic and haplotype network analyses revealed the presence of three separated haplotype clades: (1) M. baroni, M. aff. baroni, M. nigricans, and putative hybrids of M. cowani and M. baroni, (2) M. cowani and putative hybrids of M. cowani and M. baroni, and (3) M. haraldmeieri. The putative hybrids were collected from sites where M. cowani and M. baroni live in sympatry. These results suggest (a) a probable hybridization between M. cowani and M. baroni, (b) a lack of genetic differentiation between M. baroni/M. aff. baroni and M. nigricans, (c) evidence of recent gene-flow between the northern (M. nigricans), eastern (M. baroni), and south-eastern (M. aff. baroni) forms of distinct coloration, and (d) the existence of at least three units for conservation in the Mantella cowani group.
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Evidence for recent gene flow between north-eastern and south-eastern Madagascan poison frogs from a phylogeography of the Mantella cowani group
Frontiers in Zoology, 2007Co-Authors: Falitiana C E Rabemananjara, Olga Ramilijaona, Ylenia Chiari, Miguel VencesAbstract:Background The genus Mantella , endemic poison frogs of Madagascar with 16 described species, are known in the field of international pet trade and entered under the CITES control for the last four years. The phylogeny and phylogeography of this genus have been recently subject of study for conservation purposes. Here we report on the studies of the phylogeography of the Mantella cowani group using a fragment of 453 bp of the mitochondrial cytochrome b gene from 195 individuals from 21 localities. This group is represented by five forms: M. cowani , a critically endangered species, a vulnerable species, M. haraldmeieri , and the non-threatened M. baroni, M . aff. baroni , and M. nigricans . Results The Bayesian phylogenetic and haplotype network analyses revealed the presence of three separated haplotype clades: (1) M. baroni, M . aff. baroni, M. nigricans , and putative hybrids of M. cowani and M. baroni , (2) M. cowani and putative hybrids of M. cowani and M. baroni , and (3) M. haraldmeieri . The putative hybrids were collected from sites where M. cowani and M. baroni live in sympatry. Conclusion These results suggest (a) a probable hybridization between M. cowani and M. baroni , (b) a lack of genetic differentiation between M. baroni/M . aff. baroni and M. nigricans , (c) evidence of recent gene-flow between the northern ( M. nigricans ), eastern ( M. baroni ), and south-eastern ( M . aff. baroni ) forms of distinct coloration, and (d) the existence of at least three units for conservation in the Mantella cowani group.
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Frontiers in Zoology BioMed Central
2007Co-Authors: Falitiana C E Rabemananjara, Olga Ramilijaona, Ylenia Chiari, Miguel VencesAbstract:Evidence for recent gene flow between north-eastern and south-eastern Madagascan poison frogs from a phylogeography of the Mantella cowani grou
Olga Ramilijaona - One of the best experts on this subject based on the ideXlab platform.
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evidence for recent gene flow between north eastern and south eastern madagascan poison frogs from a phylogeography of the Mantella cowani group
Frontiers in Zoology, 2007Co-Authors: Falitiana C E Rabemananjara, Olga Ramilijaona, Ylenia Chiari, Miguel VencesAbstract:Background The genus Mantella, endemic poison frogs of Madagascar with 16 described species, are known in the field of international pet trade and entered under the CITES control for the last four years. The phylogeny and phylogeography of this genus have been recently subject of study for conservation purposes. Here we report on the studies of the phylogeography of the Mantella cowani group using a fragment of 453 bp of the mitochondrial cytochrome b gene from 195 individuals from 21 localities. This group is represented by five forms: M. cowani, a critically endangered species, a vulnerable species, M. haraldmeieri, and the non-threatened M. baroni, M. aff. baroni, and M. nigricans.
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evidence for recent gene flow between north eastern and south eastern madagascan poison frogs from a phylogeography of the Mantella cowani group
Frontiers in Zoology, 2007Co-Authors: Falitiana C E Rabemananjara, Olga Ramilijaona, Ylenia Chiari, Miguel VencesAbstract:The genus Mantella, endemic poison frogs of Madagascar with 16 described species, are known in the field of international pet trade and entered under the CITES control for the last four years. The phylogeny and phylogeography of this genus have been recently subject of study for conservation purposes. Here we report on the studies of the phylogeography of the Mantella cowani group using a fragment of 453 bp of the mitochondrial cytochrome b gene from 195 individuals from 21 localities. This group is represented by five forms: M. cowani, a critically endangered species, a vulnerable species, M. haraldmeieri, and the non-threatened M. baroni, M. aff. baroni, and M. nigricans. The Bayesian phylogenetic and haplotype network analyses revealed the presence of three separated haplotype clades: (1) M. baroni, M. aff. baroni, M. nigricans, and putative hybrids of M. cowani and M. baroni, (2) M. cowani and putative hybrids of M. cowani and M. baroni, and (3) M. haraldmeieri. The putative hybrids were collected from sites where M. cowani and M. baroni live in sympatry. These results suggest (a) a probable hybridization between M. cowani and M. baroni, (b) a lack of genetic differentiation between M. baroni/M. aff. baroni and M. nigricans, (c) evidence of recent gene-flow between the northern (M. nigricans), eastern (M. baroni), and south-eastern (M. aff. baroni) forms of distinct coloration, and (d) the existence of at least three units for conservation in the Mantella cowani group.
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Evidence for recent gene flow between north-eastern and south-eastern Madagascan poison frogs from a phylogeography of the Mantella cowani group
Frontiers in Zoology, 2007Co-Authors: Falitiana C E Rabemananjara, Olga Ramilijaona, Ylenia Chiari, Miguel VencesAbstract:Background The genus Mantella , endemic poison frogs of Madagascar with 16 described species, are known in the field of international pet trade and entered under the CITES control for the last four years. The phylogeny and phylogeography of this genus have been recently subject of study for conservation purposes. Here we report on the studies of the phylogeography of the Mantella cowani group using a fragment of 453 bp of the mitochondrial cytochrome b gene from 195 individuals from 21 localities. This group is represented by five forms: M. cowani , a critically endangered species, a vulnerable species, M. haraldmeieri , and the non-threatened M. baroni, M . aff. baroni , and M. nigricans . Results The Bayesian phylogenetic and haplotype network analyses revealed the presence of three separated haplotype clades: (1) M. baroni, M . aff. baroni, M. nigricans , and putative hybrids of M. cowani and M. baroni , (2) M. cowani and putative hybrids of M. cowani and M. baroni , and (3) M. haraldmeieri . The putative hybrids were collected from sites where M. cowani and M. baroni live in sympatry. Conclusion These results suggest (a) a probable hybridization between M. cowani and M. baroni , (b) a lack of genetic differentiation between M. baroni/M . aff. baroni and M. nigricans , (c) evidence of recent gene-flow between the northern ( M. nigricans ), eastern ( M. baroni ), and south-eastern ( M . aff. baroni ) forms of distinct coloration, and (d) the existence of at least three units for conservation in the Mantella cowani group.
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Frontiers in Zoology BioMed Central
2007Co-Authors: Falitiana C E Rabemananjara, Olga Ramilijaona, Ylenia Chiari, Miguel VencesAbstract:Evidence for recent gene flow between north-eastern and south-eastern Madagascan poison frogs from a phylogeography of the Mantella cowani grou
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Individual variation in alkaloid content of poison frogs of Madagascar (Mantella; Mantellidae
2006Co-Authors: Valerie C Clark, Olga Ramilijaona, Brian L Fisher, Valerie Rakotomalala, Leif Abrell, V. C. Clark, Rakotomalala :o. V. Ramilijaona, B. L. FisherAbstract:Abstract Brightly colored Malagasy poison frogs, Mantella spp., sequester lipophilic, basic alkaloids from arthropod prey for their own chemical defense. Consequently, microsympatric prey diversity is expected to influence alkaloid diversity observed in poison frogs. Twenty-two specimens of three Mantella species from four localities in moist forests of southeastern Madagascar were analyzed individually via gas chromatography-mass spectrometry, revealing that they contain over 80 known alkaloids. Frogs within a locality possessed significantly similar alkaloid content and diversity, while frogs from areas that varied in disturbance, elevation, and/or species showed greater differences. Based on dietary data, the larger frog species Mantella baroni consumed more and larger prey, and showed greater diversity in skin alkaloids than significantly smaller Mantella bernhardi. Additionally, frogs from the most pristine locality had the greatest number of alkaloids, whereas individuals from the most disturbed localities had the least. In a comparison of frog alkaloid profiles over a 10- to 14-yr period, alkaloid turnover, and thus presumably alkaloid-source arthropod turnover, was high in a disturbed locality and low in the pristine primary forest locality. We demonstrate that the nonlethal transcutaneous amphibia
Franco Andreone - One of the best experts on this subject based on the ideXlab platform.
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RABEMANANJARA,
2016Co-Authors: David R. Vieites, Miguel Vences, Franco Andreone, Sandra Nieto-román, Axel MeyerAbstract:Blackwell Publishing LtdMitochondrial evidence for distinct phylogeographic units in the endangered Malagasy poison frog Mantella bernhard
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8 Natur-Museum Luzern.
2015Co-Authors: Monografie Museo, Franco Andreone, Frank Glaw, Regionale Scienze Naturali, Zoologische Staatssammlung München, Hessisches Landesmuseum Darmstadt, Neil A. Cox, Jörn Köhler, Nirhy H. C. Rabibisoa, Herilala RandriamahazoAbstract:for Madagascar in light of species discoveries, nomenclature changes, and new field information We updated the Global Amphibian Assessment for Malagasy amphibians, including considerations on the species described or resurrected between 2005-2007, and newly available information on other taxa. The revised assessment allowed us to include 66 threatened species: 6 Critically Endangered, 31 Endangered, and 29 Vulnerable. Three species formerly assessed as Critically Endangered (Mantella expectata, M. viridis, and Scaphiophryne gottlebei) are downlisted to Endangered, since they are more widespread than formerly presumed. Other recently described species have been assessed as threatened: seven are categorised as Endangered (Boophis tampoka, Gephyromantis azzurrae, G. runewsweeki, Mantidactylus noralottae, Tsingymantis antitra, Cophyla berara, and Plethodontohyla fonetana), and a single species (Boophis sambirano) categorised as Vulnerable. The little known and enigmatic Mantella manery, formerly assessed as Data Deficient, has been recently found on the Tsaratanana Massif. In view of this enlarged distribution and ongoing degradation of the habitats where it lives, it has been re-assessed as Vulnerable
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Chromosome Data for Malagasy Poison Frogs (Amphibia: Ranidae: Mantella) and Their Bearing on Taxonomy and Phylogeny
2014Co-Authors: Gaetano Odierna, Miguel Vences, Gennaro Aprea, Stefan Lötters, Franco AndreoneAbstract:ABSTRACT—We compared chromosome morphologies for 11 species of Malagasy poison frogs, genus Mantella, and three outgroup taxa (genus Mantidactylus) using conventional and fluorescence staining tech-niques. All species studied had a karyotype of 2n=26, with five larger and eight smaller chromosome pairs. The 11th pair was acrocentic in Mantella nigricans which represents the first such observation in the genus. The nucleolus organizer region (NOR) was located at secondary constrictions on chromosome pair 2 in all Mantella studied and in Mantidactylus grandisonae (while located on other chromosomes in all other species of Mantidactylus studied so far). Heterochromatin distribution was highly variable among Mantella species; C-bands positively staining with DAPI and CMA3 were observed. The possible structure of these bands, seemingly containing both A+T rich and C+G rich heterochromatin, is discussed. Phylogenetic reconstruc-tion using chromosomal characters provided very little information. Evolution of the characters studied is probably either too fast (heterochromatin arrangement) or too slow (NOR location) to match the main cladogenetic events among Mantella species groups
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phylogeography of the poison frog Mantella viridis amphibia mantellidae reveals chromatic and genetic differentiation across ecotones in northern madagascar
Journal of Zoological Systematics and Evolutionary Research, 2012Co-Authors: Angelica Crottini, Miguel Vences, Vincenzo Mercurio, Jason L Brown, Frank Glaw, Franco AndreoneAbstract:Mantella viridis is a threatened poison frog species endemic to the ecologically very heterogeneous northern region of Madagascar. The existence of several colour morphs within M. viridis and its very low genetic differentiation to the allopatrically distributed Mantella ebenaui raise questions about the processes driving the differentiation between these poison frog populations and about their taxonomic status. Using a DNA fragment of 476 nucleotides of the mitochondrial cytochrome b gene from 240 individuals of this species complex, we investigated the genetic variability of all known colour morphs of M. viridis, sampling this species throughout its known range, as well as several populations of M. ebenaui. Our genetic results confirm that M. viridis and M. ebenaui are closely related but reveal that no haplotype sharing occurs between these two taxa. Further, our molecular analyses provided evidence for barriers to gene flow among some of the colour morphs. Estimates of overlap of bioclimatic envelopes as assessed by ecological niche modelling also suggest a distinct bioclimatic niche of some of the lineages studied.
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phylogeography of the poison frog Mantella viridis amphibia mantellidae reveals chromatic and genetic differentiation across ecotones in northern madagascarphylogeographische untersuchungen an einem madagassischen giftfrosch Mantella viridis amphibia mantellidae belegen genetische differenzierung und unterschiede im farbmuster uber einen okologischen gradienten in nordmadagaskar
Journal of Zoological Systematics and Evolutionary Research, 2012Co-Authors: Angelica Crottini, Miguel Vences, Vincenzo Mercurio, Jason L Brown, Frank Glaw, Franco AndreoneAbstract:Mantella viridis is a threatened poison frog species endemic to the ecologically very heterogeneous northern region of Madagascar. The existence of several colour morphs within M. viridis and its very low genetic differentiation to the allopatrically distributed Mantella ebenaui raise questions about the processes driving the differentiation between these poison frog populations and about their taxonomic status. Using a DNA fragment of 476 nucleotides of the mitochondrial cytochrome b gene from 240 individuals of this species complex, we investigated the genetic variability of all known colour morphs of M. viridis, sampling this species throughout its known range, as well as several populations of M. ebenaui. Our genetic results confirm that M. viridis and M. ebenaui are closely related but reveal that no haplotype sharing occurs between these two taxa. Further, our molecular analyses provided evidence for barriers to gene flow among some of the colour morphs. Estimates of overlap of bioclimatic envelopes as assessed by ecological niche modelling also suggest a distinct bioclimatic niche of some of the lineages studied. Zusammenfassung Mantella viridis ist eine bedrohte Art madagassischer Giftfrosche, deren Verbreitung auf die okologisch sehr heterogene Nordspitze Madagaskars beschrankt ist. Jungere Untersuchungen haben unterschiedliche Farbmorphen von M. viridis und eine anscheinend sehr geringe genetische Distanz der Art zu ihrer Schwesterart M. ebenaui nachgewiesen. Dies wirft Fragen zum taxonomischen Status dieser Populationen und zu den Differenzierungsprozessen auf, die zu ihrer Ausbildung in Nordmadagaskar gefuhrt haben. Um zur Klarung dieser Fragen beizusteuern, untersuchten wir die genetische Variabilitat und Differenzierung von M. viridis und M. ebenaui auf der Basis von DNA Sequenzen des mitochondrialen Cytochrome b-Gens (476 Nukleotide) von insgesamt 240 Individuen, unter Abdeckung des gesamten Verbreitungsgebiets und aller bekannten Populationen von M. viridis. Unsere Ergebnisse bestatigen die geringe Auspragung mitochondrialer Unterschiede zwischen M. ebenaui und M. viridis, obwohl beide Arten reziprok monophyletische Linien bilden und keine gemeinsamen Haplotypen besitzen. Trotz gemeinsamer Haploytpen zwischen mehrerer der anderen Farbmorphen in Nordmadagaskar und dem Nachweis von Isolationsprozessen abhangig von der geographischen Distanz konnten unsere Analysen Genfluss-Barrieren zwischen einigen Farbmorphen von M. viridis nachweisen. Zudem zeigten Analysen ihrer bioklimatischen Nischen, dass M. ebenaui in bioklimatisch unterschiedlichen Gebieten im Vergleich zu den anderen Farbmorphen lebt. Schlusselworter: Amphibia: Mantellidae: Mantella viridis, Madagaskar, Mikroendemismus, adaptive Diversifizierung, Cytochrome b.