The Experts below are selected from a list of 5727 Experts worldwide ranked by ideXlab platform
Jin Ho Lee - One of the best experts on this subject based on the ideXlab platform.
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bioactive porous beads as an injectable urethral bulking agent in vivo animal study for the treatment of urinary incontinence
Tissue Engineering Part A, 2011Co-Authors: In Gul Kim, Ji Young Lee, Ji Youl Lee, Jin Ho LeeAbstract:In our previous study, growth factor (basic fibroblast growth factor [bFGF] or vascular endothelial growth factor)-immobilized polycaprolactone (PCL)/Pluronic F127 porous beads were fabricated by an isolated particle-Melting/Melt-Molding particulate-leaching method. The growth factors were easily immobilized onto the pore surfaces of the PCL/F127 beads via heparin binding, and were continuously released for up to 28 days. In this study, the growth factor-immobilized porous beads were investigated for their potential use as an injectable urethral bulking agent for the treatment of stress urinary incontinence (SUI). From the in vivo study using Sprague-Dawley rats as an urinary incontinent animal model, it was observed that the growth factor (bFGF or vascular endothelial growth factor)-immobilized porous beads had effective cure behaviors for SUI as follows: the narrowed urethral lumen and the regeneration of smooth muscle around the urethra. In particular, the bFGF-immobilized PCL/F127 porous beads showed ...
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bioactive porous beads as an injectable urethral bulking agent their in vitro evaluation on smooth muscle cell differentiation
Tissue Engineering Part A, 2011Co-Authors: In Gul Kim, Ji Young Lee, Ji Youl Lee, Jin Ho LeeAbstract:Growth factor (basic fibroblast growth factor or vascular endothelial growth factor)-immobilized polycaprolactone (PCL)/Pluronic F127 porous beads were prepared as an injectable bulking agent for effective treatment of urinary incontinence. The growth factor-immobilized porous beads may stimulate smooth muscle cell (SMC) differentiation of muscle-derived stem cells or defect tissues around urethra to improve the sphincter function (bioactive therapy) as well as to provide a bulking effect (passive therapy). The porous PCL/F127 beads were fabricated by an isolated particle-Melting/Melt-Molding particulate-leaching method. The growth factors were easily immobilized onto the surfaces of the PCL/F127 porous beads via heparin binding and were continuously released for up to 28 days. Both growth factor-immobilized porous beads had a positive effect for the SMC differentiation of muscle-derived stem cells, as were demonstrated by the analyses of quantitative polymerase chain reactions, Western blot using SMC-spe...
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degradation behavior of hydrophilized plga scaffolds prepared by Melt Molding particulate leaching method comparison with control hydrophobic one
Journal of Materials Science: Materials in Medicine, 2006Co-Authors: Soung Gon Kang, Jin Ho LeeAbstract:Porous PLGA/PVA scaffolds as hydrophilized PLGA scaffolds for tissue engineering applications were fabricated by a novel Melt-Molding particulate leaching method (non-solvent method). The prepared scaffolds exhibited highly porous and open-cellular pore structures with almost same surface and interior porosities (pore size, 200–300 μ m; porosity, about 90%). The in vitro degradation behavior of the PLGA and PLGA/PVA scaffolds was compared at 37∘C in PBS (pH 7.4) with and without the solution change everyday to see the effect of solution pH as well as scaffold hydrophilicity on the degradation behavior. The changes in dimension, molecular weight, mechanical properties (maximum load and modulus), and morphology of the scaffolds were examined with degradation time. The degradation behavior of the PLGA and PLGA/PVA scaffolds was further investigated in vivousing a rat model (subcutaneously implantation). It was observed that both PLGA and PLGA/PVA scaffolds in decreasing pH condition (PBS no change) showed faster degradation than those in constant pH condition (PBS change everyday), owing to the enhanced intramolecular depolymerization by the increment of chain hydrophilicity caused by carboxylate groups as well as the autocatalysis of carboxylic acids accumulated in the solution by the cleavage of PLGA backbone ester bonds. The scaffolds in vivo condition also showed faster degradation than those in vitro, probably due to the aid of foreign body giant cells or enzymes. The PLGA/PVA scaffold showed slightly faster degradation than the PLGA scaffold for both in vitro and in vivo conditions.
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fabrication and characterization of hydrophilic poly lactic co glycolic acid poly vinyl alcohol blend cell scaffolds by Melt Molding particulate leaching method
Biomaterials, 2003Co-Authors: Soung Gon Kang, Eun Kim, Sang Ho Cho, Jin Ho LeeAbstract:Porous PLGA/PVA scaffolds were fabricated by blending poly(lactic-co-glycolic acid) (PLGA) with polyvinyl alcohol (PVA) to improve the hydrophilicity and cell compatibility of the scaffolds for tissue engineering applications. PLGA/PVA blend scaffolds with different PVA compositions up to 20wt% were fabricated by a Melt-Molding particulate-leaching method (non-solvent method). The prepared scaffolds were investigated by scanning electron microscopy (SEM), mercury intrusion porosimetry, the measurements of water contact angles and bi-axial tensile strengths, etc. for their surface and bulk characterizations. The scaffolds exhibited highly porous and open-cellular pore structures with almost same surface and interior porosities (pore size, 200-300 microm; porosity, about 90%). The PLGA/PVA blend scaffolds with PVA compositions more than 5% were easily wetted in cell culture medium without any prewetting treatments, which is highly desirable for tissue engineering applications. In vitro cell compatibility of the control hydrophobic PLGA and hydrophilized PLGA/PVA (5wt%) blend scaffolds was compared by the culture of human chondrocytes in the scaffolds and the following analyses by MTT assay and SEM observation. It was observed that the PLGA/PVA blend scaffold had better cell adhesion and growth than the control PLGA scaffold. For in vivo evaluation of tissue compatibility, the scaffolds were implanted into the skull defects of rabbits. The results were evaluated by histology examinations. The PLGA/PVA (5wt%) blend scaffold showed better bone ingrowth into the scaffold and new bone formation inside the scaffold than the PLGA scaffold. It seems that 5% addition of PVA to PLGA to fabricate PLGA/PVA blend scaffolds is enough for improving the hydrophilicity and cell compatibility of the scaffolds.
Wei Wang - One of the best experts on this subject based on the ideXlab platform.
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porous polycaprolactone nanohydroxyapatite tissue engineering scaffolds fabricated by combining nacl and peg as co porogens structure property and chondrocyte scaffold interaction in vitro
Journal of Biomedical Materials Research Part B, 2012Co-Authors: Yuanyuan Wang, Zhenyu Wang, Wei WangAbstract:In this study, porous polycaprolactone/nanohydroxyapatite (PCL/nHA) composite scaffolds were fabricated using a modified Melt-Molding/leaching technique, by the combination of salt particulate (NaCl) and water-soluble polymer (PEG) as co-porogens. The porogens were kept at a constant proportion of 70% in the blends but varied in the NaCl/PEG ratio and the PEG variety to generate PCL/nHA scaffolds with various pore architectures. The resultant composite scaffolds were investigated on their morphologies, physicochemical properties, mechanical properties, and in vitro degradation. The cell–scaffold interactions were evaluated in vitro using chondrocyte. Generally, the PCL/nHA scaffolds exhibited multimodal pore morphologies consisting of macropores and interconnected micropores, created by the extraction of NaCl particulate and continuous PEG phase. The evolution of porogens led to much effect on the overall pore architecture of the scaffolds; subsequently, their physiochemical and mechanical properties and degradation behaviors, as well as the cell binding and proliferation. The PCL/nHA scaffold prepared from NaCl/PEG 4000 (20/50) presented more macropores (>50 μm) with interconnectivity and showed higher strength and improved bioactivity than the others. All of these results suggest promising potentials of PCL/nHA scaffolds developed in this study desired for cartilage tissue engineering. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2012.
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porous polycaprolactone nanohydroxyapatite tissue engineering scaffolds fabricated by combining nacl and peg as co porogens structure property and chondrocyte scaffold interaction in vitro
Journal of Biomedical Materials Research Part B, 2012Co-Authors: Li Liu, Yuanyuan Wang, Zhenyu Wang, Shengrong Guo, Wei WangAbstract:In this study, porous polycaprolactone/nanohydroxyapatite (PCL/nHA) composite scaffolds were fabricated using a modified Melt-Molding/leaching technique, by the combination of salt particulate (NaCl) and water-soluble polymer (PEG) as co-porogens. The porogens were kept at a constant proportion of 70% in the blends but varied in the NaCl/PEG ratio and the PEG variety to generate PCL/nHA scaffolds with various pore architectures. The resultant composite scaffolds were investigated on their morphologies, physicochemical properties, mechanical properties, and in vitro degradation. The cell-scaffold interactions were evaluated in vitro using chondrocyte. Generally, the PCL/nHA scaffolds exhibited multimodal pore morphologies consisting of macropores and interconnected micropores, created by the extraction of NaCl particulate and continuous PEG phase. The evolution of porogens led to much effect on the overall pore architecture of the scaffolds; subsequently, their physiochemical and mechanical properties and degradation behaviors, as well as the cell binding and proliferation. The PCL/nHA scaffold prepared from NaCl/PEG 4000 (20/50) presented more macropores (>50 μm) with interconnectivity and showed higher strength and improved bioactivity than the others. All of these results suggest promising potentials of PCL/nHA scaffolds developed in this study desired for cartilage tissue engineering.
Yuanyuan Wang - One of the best experts on this subject based on the ideXlab platform.
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porous polycaprolactone nanohydroxyapatite tissue engineering scaffolds fabricated by combining nacl and peg as co porogens structure property and chondrocyte scaffold interaction in vitro
Journal of Biomedical Materials Research Part B, 2012Co-Authors: Yuanyuan Wang, Zhenyu Wang, Wei WangAbstract:In this study, porous polycaprolactone/nanohydroxyapatite (PCL/nHA) composite scaffolds were fabricated using a modified Melt-Molding/leaching technique, by the combination of salt particulate (NaCl) and water-soluble polymer (PEG) as co-porogens. The porogens were kept at a constant proportion of 70% in the blends but varied in the NaCl/PEG ratio and the PEG variety to generate PCL/nHA scaffolds with various pore architectures. The resultant composite scaffolds were investigated on their morphologies, physicochemical properties, mechanical properties, and in vitro degradation. The cell–scaffold interactions were evaluated in vitro using chondrocyte. Generally, the PCL/nHA scaffolds exhibited multimodal pore morphologies consisting of macropores and interconnected micropores, created by the extraction of NaCl particulate and continuous PEG phase. The evolution of porogens led to much effect on the overall pore architecture of the scaffolds; subsequently, their physiochemical and mechanical properties and degradation behaviors, as well as the cell binding and proliferation. The PCL/nHA scaffold prepared from NaCl/PEG 4000 (20/50) presented more macropores (>50 μm) with interconnectivity and showed higher strength and improved bioactivity than the others. All of these results suggest promising potentials of PCL/nHA scaffolds developed in this study desired for cartilage tissue engineering. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2012.
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porous polycaprolactone nanohydroxyapatite tissue engineering scaffolds fabricated by combining nacl and peg as co porogens structure property and chondrocyte scaffold interaction in vitro
Journal of Biomedical Materials Research Part B, 2012Co-Authors: Li Liu, Yuanyuan Wang, Zhenyu Wang, Shengrong Guo, Wei WangAbstract:In this study, porous polycaprolactone/nanohydroxyapatite (PCL/nHA) composite scaffolds were fabricated using a modified Melt-Molding/leaching technique, by the combination of salt particulate (NaCl) and water-soluble polymer (PEG) as co-porogens. The porogens were kept at a constant proportion of 70% in the blends but varied in the NaCl/PEG ratio and the PEG variety to generate PCL/nHA scaffolds with various pore architectures. The resultant composite scaffolds were investigated on their morphologies, physicochemical properties, mechanical properties, and in vitro degradation. The cell-scaffold interactions were evaluated in vitro using chondrocyte. Generally, the PCL/nHA scaffolds exhibited multimodal pore morphologies consisting of macropores and interconnected micropores, created by the extraction of NaCl particulate and continuous PEG phase. The evolution of porogens led to much effect on the overall pore architecture of the scaffolds; subsequently, their physiochemical and mechanical properties and degradation behaviors, as well as the cell binding and proliferation. The PCL/nHA scaffold prepared from NaCl/PEG 4000 (20/50) presented more macropores (>50 μm) with interconnectivity and showed higher strength and improved bioactivity than the others. All of these results suggest promising potentials of PCL/nHA scaffolds developed in this study desired for cartilage tissue engineering.
Zhenyu Wang - One of the best experts on this subject based on the ideXlab platform.
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porous polycaprolactone nanohydroxyapatite tissue engineering scaffolds fabricated by combining nacl and peg as co porogens structure property and chondrocyte scaffold interaction in vitro
Journal of Biomedical Materials Research Part B, 2012Co-Authors: Yuanyuan Wang, Zhenyu Wang, Wei WangAbstract:In this study, porous polycaprolactone/nanohydroxyapatite (PCL/nHA) composite scaffolds were fabricated using a modified Melt-Molding/leaching technique, by the combination of salt particulate (NaCl) and water-soluble polymer (PEG) as co-porogens. The porogens were kept at a constant proportion of 70% in the blends but varied in the NaCl/PEG ratio and the PEG variety to generate PCL/nHA scaffolds with various pore architectures. The resultant composite scaffolds were investigated on their morphologies, physicochemical properties, mechanical properties, and in vitro degradation. The cell–scaffold interactions were evaluated in vitro using chondrocyte. Generally, the PCL/nHA scaffolds exhibited multimodal pore morphologies consisting of macropores and interconnected micropores, created by the extraction of NaCl particulate and continuous PEG phase. The evolution of porogens led to much effect on the overall pore architecture of the scaffolds; subsequently, their physiochemical and mechanical properties and degradation behaviors, as well as the cell binding and proliferation. The PCL/nHA scaffold prepared from NaCl/PEG 4000 (20/50) presented more macropores (>50 μm) with interconnectivity and showed higher strength and improved bioactivity than the others. All of these results suggest promising potentials of PCL/nHA scaffolds developed in this study desired for cartilage tissue engineering. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2012.
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porous polycaprolactone nanohydroxyapatite tissue engineering scaffolds fabricated by combining nacl and peg as co porogens structure property and chondrocyte scaffold interaction in vitro
Journal of Biomedical Materials Research Part B, 2012Co-Authors: Li Liu, Yuanyuan Wang, Zhenyu Wang, Shengrong Guo, Wei WangAbstract:In this study, porous polycaprolactone/nanohydroxyapatite (PCL/nHA) composite scaffolds were fabricated using a modified Melt-Molding/leaching technique, by the combination of salt particulate (NaCl) and water-soluble polymer (PEG) as co-porogens. The porogens were kept at a constant proportion of 70% in the blends but varied in the NaCl/PEG ratio and the PEG variety to generate PCL/nHA scaffolds with various pore architectures. The resultant composite scaffolds were investigated on their morphologies, physicochemical properties, mechanical properties, and in vitro degradation. The cell-scaffold interactions were evaluated in vitro using chondrocyte. Generally, the PCL/nHA scaffolds exhibited multimodal pore morphologies consisting of macropores and interconnected micropores, created by the extraction of NaCl particulate and continuous PEG phase. The evolution of porogens led to much effect on the overall pore architecture of the scaffolds; subsequently, their physiochemical and mechanical properties and degradation behaviors, as well as the cell binding and proliferation. The PCL/nHA scaffold prepared from NaCl/PEG 4000 (20/50) presented more macropores (>50 μm) with interconnectivity and showed higher strength and improved bioactivity than the others. All of these results suggest promising potentials of PCL/nHA scaffolds developed in this study desired for cartilage tissue engineering.
Soung Gon Kang - One of the best experts on this subject based on the ideXlab platform.
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degradation behavior of hydrophilized plga scaffolds prepared by Melt Molding particulate leaching method comparison with control hydrophobic one
Journal of Materials Science: Materials in Medicine, 2006Co-Authors: Soung Gon Kang, Jin Ho LeeAbstract:Porous PLGA/PVA scaffolds as hydrophilized PLGA scaffolds for tissue engineering applications were fabricated by a novel Melt-Molding particulate leaching method (non-solvent method). The prepared scaffolds exhibited highly porous and open-cellular pore structures with almost same surface and interior porosities (pore size, 200–300 μ m; porosity, about 90%). The in vitro degradation behavior of the PLGA and PLGA/PVA scaffolds was compared at 37∘C in PBS (pH 7.4) with and without the solution change everyday to see the effect of solution pH as well as scaffold hydrophilicity on the degradation behavior. The changes in dimension, molecular weight, mechanical properties (maximum load and modulus), and morphology of the scaffolds were examined with degradation time. The degradation behavior of the PLGA and PLGA/PVA scaffolds was further investigated in vivousing a rat model (subcutaneously implantation). It was observed that both PLGA and PLGA/PVA scaffolds in decreasing pH condition (PBS no change) showed faster degradation than those in constant pH condition (PBS change everyday), owing to the enhanced intramolecular depolymerization by the increment of chain hydrophilicity caused by carboxylate groups as well as the autocatalysis of carboxylic acids accumulated in the solution by the cleavage of PLGA backbone ester bonds. The scaffolds in vivo condition also showed faster degradation than those in vitro, probably due to the aid of foreign body giant cells or enzymes. The PLGA/PVA scaffold showed slightly faster degradation than the PLGA scaffold for both in vitro and in vivo conditions.
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fabrication and characterization of hydrophilic poly lactic co glycolic acid poly vinyl alcohol blend cell scaffolds by Melt Molding particulate leaching method
Biomaterials, 2003Co-Authors: Soung Gon Kang, Eun Kim, Sang Ho Cho, Jin Ho LeeAbstract:Porous PLGA/PVA scaffolds were fabricated by blending poly(lactic-co-glycolic acid) (PLGA) with polyvinyl alcohol (PVA) to improve the hydrophilicity and cell compatibility of the scaffolds for tissue engineering applications. PLGA/PVA blend scaffolds with different PVA compositions up to 20wt% were fabricated by a Melt-Molding particulate-leaching method (non-solvent method). The prepared scaffolds were investigated by scanning electron microscopy (SEM), mercury intrusion porosimetry, the measurements of water contact angles and bi-axial tensile strengths, etc. for their surface and bulk characterizations. The scaffolds exhibited highly porous and open-cellular pore structures with almost same surface and interior porosities (pore size, 200-300 microm; porosity, about 90%). The PLGA/PVA blend scaffolds with PVA compositions more than 5% were easily wetted in cell culture medium without any prewetting treatments, which is highly desirable for tissue engineering applications. In vitro cell compatibility of the control hydrophobic PLGA and hydrophilized PLGA/PVA (5wt%) blend scaffolds was compared by the culture of human chondrocytes in the scaffolds and the following analyses by MTT assay and SEM observation. It was observed that the PLGA/PVA blend scaffold had better cell adhesion and growth than the control PLGA scaffold. For in vivo evaluation of tissue compatibility, the scaffolds were implanted into the skull defects of rabbits. The results were evaluated by histology examinations. The PLGA/PVA (5wt%) blend scaffold showed better bone ingrowth into the scaffold and new bone formation inside the scaffold than the PLGA scaffold. It seems that 5% addition of PVA to PLGA to fabricate PLGA/PVA blend scaffolds is enough for improving the hydrophilicity and cell compatibility of the scaffolds.