The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Lingxin Chen - One of the best experts on this subject based on the ideXlab platform.
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strategies of Molecular Imprinting based solid phase extraction prior to chromatographic analysis
Trends in Analytical Chemistry, 2020Co-Authors: Maryam Arabi, Lingxin Chen, Xiaoyan Wang, Abbas Ostovan, Ahmad Bagheri, Xiaotong Guo, Liyan WangAbstract:Abstract Molecular Imprinting-based solid-phase extraction (MI-SPE) has been in the spotlight to improve the recognition selectivity and detection sensitivity. MI-SPE provides a powerful tool for chemo/bioanalysis in complex matrices and meanwhile, benefits from distinguished advantages such as easy operation, high throughput, low cost, high selectivity and durability. This review proposed the recent advances in Molecular Imprinting concerning novel preparation strategies of Molecularly imprinted polymers (MIPs) and typical applications of MI-SPE. Preparation strategies are highlighted by dividing into ten sections mainly including dummy Imprinting, multi-template Imprinting, surface Imprinting, water-compatible Imprinting, restricted access material combining Imprinting etc.; each section provides the descriptions about what restrictions led to the emergence of any strategy, strengths/weaknesses of every strategy and universal applications of upgraded MIPs in various SPE modes prior to chromatographic analysis. The potential of MIPs for implementation in routine laboratory activities and scale-up is expected, and finally remaining challenges and future perspectives are proposed.
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Molecular Imprinting technology for microorganism analysis
Trends in Analytical Chemistry, 2018Co-Authors: Mengfan Jia, Lingxin Chen, Zhong Zhang, Xingbin YangAbstract:Abstract Molecular Imprinting technology has been widely applied to various fields, owing to unique features of structure predictability, recognition specificity and application universality. Microorganism Imprinting has attracted significant interests attributing to the high selectivity, simplicity rapidity, and excellent stability as well as low cost and eco-friendliness. Herein, we purpose to review the recent advances of MIT for microorganism analysis, concerning Imprinting methods, analytical detection methods and typical applications. Various Imprinting methods including direct and indirect Imprinting for microorganism-MIPs preparation are comprehensively summarized. MIPs based biosensors containing fluorescence, electrochemical, piezoelectric and surface plasmon resonance for analytical detection of microorganisms is highlighted. Representative applications of microbiological Imprinting are discussed, involving detection and quantification of bacteria, identification of bacterial species, and determination of yeast growth status. Finally, we propose the remaining challenges and future perspectives to accelerate the development and utilization of MIT in microorganism analysis and thereby push forwards microorganism identification and determination.
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rotational paper based microfluidic chip device for multiplexed and simultaneous fluorescence detection of phenolic pollutants based on a Molecular Imprinting technique
Analytical Chemistry, 2018Co-Authors: Xiaoyan Wang, Lingxin Chen, Liqiang LuoAbstract:In this study, we first present rotational paper-based microfluidic chips (RPADs) combined with a Molecular-Imprinting (MIP) technique to detect phenolic pollutants. The proposed rotational paper-based microfluidic chips could implement qualitative and quantitative analysis of two different phenolic contaminants, 4-nitrophenol (4-NP) and 2,4,6-trinitrophenol (TNP), simultaneously. Qualitative and quantitative analysis could be implemented simultaneously through fluorescence-intensity changes depending on the structures of quantum dots combined with a Molecular-Imprinting technique. Moreover, the rotational paper-based microfluidic chips provide a low cost, flexible, and easy way to operate the entire process conveniently. Under the optimal conditions, the proposed sensors showed high sensitivity and selectivity. Our final experimental results illustrated that the detection limits of 4-NP and TNP in the paper-based quantum-dot MIP (PQ-MIP) RPADs ranged from 0.5 to 20.0 mg/L, with detection limits of 0.097 ...
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Strategies of Molecular Imprinting-based fluorescence sensors for chemical and biological analysis.
Biosensors & bioelectronics, 2018Co-Authors: Qian Yang, Xiaoyan Wang, Hailong Peng, Hua Xiong, Lingxin ChenAbstract:One pressing concern today is to construct sensors that can withstand various disturbances for highly selective and sensitive detecting trace analytes in complicated samples. Molecularly imprinted polymers (MIPs) with tailor-made binding sites are preferred to be recognition elements in sensors for effective targets detection, and fluorescence measurement assists in highly sensitive detection and user-friendly control. Accordingly, Molecular Imprinting-based fluorescence sensors (MI-FL sensors) have attracted great research interest in many fields such as chemical and biological analysis. Herein, we comprehensively review the recent advances in MI-FL sensors construction and applications, giving insights on sensing principles and signal transduction mechanisms, focusing on general construction strategies for intrinsically fluorescent or nonfluorescent analytes and improvement strategies in sensing performance, particularly in sensitivity. Construction strategies are well overviewed, mainly including the traditional indirect methods of competitive binding against pre-bound fluorescent indicators, employment of fluorescent functional monomers and embedding of fluorescence substances, and novel rational designs of hierarchical architecture (core-shell/hollow and mesoporous structures), post-Imprinting modification, and ratiometric fluorescence detection. Furthermore, MI-FL sensor based microdevices are discussed, involving micromotors, test strips and microfluidics, which are more portable for rapid point-of-care detection and in-field diagnosing. Finally, the current challenges and future perspectives of MI-FL sensors are proposed.
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Molecular Imprinting based hybrid ratiometric fluorescence sensor for the visual determination of bovine hemoglobin
ACS Sensors, 2018Co-Authors: Xiaoyan Wang, Shuangmei Yu, Longwen Fu, Jinhua Li, Yunqing Wang, Lingxin ChenAbstract:We describe a simple and effective strategy to construct a Molecular Imprinting ratiometric fluorescence sensor (MIR sensor) for the visual detection of bovine hemoglobin (BHb) used as a model protein. The sensor was prepared by simply mixing the solution of green and red CdTe quantum dots (QDs), which were embedded in core–shell structured Molecularly imprinted polymers and silica nanoparticles, respectively. The resultant hybrid MIR sensor can selectively bind with BHb and thus quench the fluorescence of the green QDs, while the red QDs wrapped with silica are insensitive to BHb with the fluorescence intensity unchanged. As a result, a continuous obvious fluorescence color change from green to red can be observed by naked eyes, with the detection limit of 9.6 nM. Moreover, the MIR sensor was successfully applied to determine BHb in bovine urine samples with satisfactory recoveries at three spiking levels ranging from 95.7 to 101.5%, indicating great potential application for detecting BHb in real sample...
Xiaoyan Wang - One of the best experts on this subject based on the ideXlab platform.
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strategies of Molecular Imprinting based solid phase extraction prior to chromatographic analysis
Trends in Analytical Chemistry, 2020Co-Authors: Maryam Arabi, Lingxin Chen, Xiaoyan Wang, Abbas Ostovan, Ahmad Bagheri, Xiaotong Guo, Liyan WangAbstract:Abstract Molecular Imprinting-based solid-phase extraction (MI-SPE) has been in the spotlight to improve the recognition selectivity and detection sensitivity. MI-SPE provides a powerful tool for chemo/bioanalysis in complex matrices and meanwhile, benefits from distinguished advantages such as easy operation, high throughput, low cost, high selectivity and durability. This review proposed the recent advances in Molecular Imprinting concerning novel preparation strategies of Molecularly imprinted polymers (MIPs) and typical applications of MI-SPE. Preparation strategies are highlighted by dividing into ten sections mainly including dummy Imprinting, multi-template Imprinting, surface Imprinting, water-compatible Imprinting, restricted access material combining Imprinting etc.; each section provides the descriptions about what restrictions led to the emergence of any strategy, strengths/weaknesses of every strategy and universal applications of upgraded MIPs in various SPE modes prior to chromatographic analysis. The potential of MIPs for implementation in routine laboratory activities and scale-up is expected, and finally remaining challenges and future perspectives are proposed.
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rotational paper based microfluidic chip device for multiplexed and simultaneous fluorescence detection of phenolic pollutants based on a Molecular Imprinting technique
Analytical Chemistry, 2018Co-Authors: Xiaoyan Wang, Lingxin Chen, Liqiang LuoAbstract:In this study, we first present rotational paper-based microfluidic chips (RPADs) combined with a Molecular-Imprinting (MIP) technique to detect phenolic pollutants. The proposed rotational paper-based microfluidic chips could implement qualitative and quantitative analysis of two different phenolic contaminants, 4-nitrophenol (4-NP) and 2,4,6-trinitrophenol (TNP), simultaneously. Qualitative and quantitative analysis could be implemented simultaneously through fluorescence-intensity changes depending on the structures of quantum dots combined with a Molecular-Imprinting technique. Moreover, the rotational paper-based microfluidic chips provide a low cost, flexible, and easy way to operate the entire process conveniently. Under the optimal conditions, the proposed sensors showed high sensitivity and selectivity. Our final experimental results illustrated that the detection limits of 4-NP and TNP in the paper-based quantum-dot MIP (PQ-MIP) RPADs ranged from 0.5 to 20.0 mg/L, with detection limits of 0.097 ...
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Strategies of Molecular Imprinting-based fluorescence sensors for chemical and biological analysis.
Biosensors & bioelectronics, 2018Co-Authors: Qian Yang, Xiaoyan Wang, Hailong Peng, Hua Xiong, Lingxin ChenAbstract:One pressing concern today is to construct sensors that can withstand various disturbances for highly selective and sensitive detecting trace analytes in complicated samples. Molecularly imprinted polymers (MIPs) with tailor-made binding sites are preferred to be recognition elements in sensors for effective targets detection, and fluorescence measurement assists in highly sensitive detection and user-friendly control. Accordingly, Molecular Imprinting-based fluorescence sensors (MI-FL sensors) have attracted great research interest in many fields such as chemical and biological analysis. Herein, we comprehensively review the recent advances in MI-FL sensors construction and applications, giving insights on sensing principles and signal transduction mechanisms, focusing on general construction strategies for intrinsically fluorescent or nonfluorescent analytes and improvement strategies in sensing performance, particularly in sensitivity. Construction strategies are well overviewed, mainly including the traditional indirect methods of competitive binding against pre-bound fluorescent indicators, employment of fluorescent functional monomers and embedding of fluorescence substances, and novel rational designs of hierarchical architecture (core-shell/hollow and mesoporous structures), post-Imprinting modification, and ratiometric fluorescence detection. Furthermore, MI-FL sensor based microdevices are discussed, involving micromotors, test strips and microfluidics, which are more portable for rapid point-of-care detection and in-field diagnosing. Finally, the current challenges and future perspectives of MI-FL sensors are proposed.
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Molecular Imprinting based hybrid ratiometric fluorescence sensor for the visual determination of bovine hemoglobin
ACS Sensors, 2018Co-Authors: Xiaoyan Wang, Shuangmei Yu, Longwen Fu, Jinhua Li, Yunqing Wang, Lingxin ChenAbstract:We describe a simple and effective strategy to construct a Molecular Imprinting ratiometric fluorescence sensor (MIR sensor) for the visual detection of bovine hemoglobin (BHb) used as a model protein. The sensor was prepared by simply mixing the solution of green and red CdTe quantum dots (QDs), which were embedded in core–shell structured Molecularly imprinted polymers and silica nanoparticles, respectively. The resultant hybrid MIR sensor can selectively bind with BHb and thus quench the fluorescence of the green QDs, while the red QDs wrapped with silica are insensitive to BHb with the fluorescence intensity unchanged. As a result, a continuous obvious fluorescence color change from green to red can be observed by naked eyes, with the detection limit of 9.6 nM. Moreover, the MIR sensor was successfully applied to determine BHb in bovine urine samples with satisfactory recoveries at three spiking levels ranging from 95.7 to 101.5%, indicating great potential application for detecting BHb in real sample...
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a Molecular Imprinting based turn on ratiometric fluorescence sensor for highly selective and sensitive detection of 2 4 dichlorophenoxyacetic acid 2 4 d
Biosensors and Bioelectronics, 2016Co-Authors: Xiaoyan Wang, Qi Kang, Dazhong Shen, Lingxin ChenAbstract:A novel Molecular Imprinting-based turn-on ratiometric fluorescence sensor was constructed via a facile sol-gel polymerization for detection of 2,4-dichlorophenoxyacetic acid (2,4-D) on the basis of photoinduced electron transfer (PET) by using nitrobenzoxadiazole (NBD) as detection signal source and quantum dots (QDs) as reference signal source. With the presence and increase of 2,4-D, the amine groups on the surface of QDs@SiO2 could bind with 2,4-D and thereby the NBD fluorescence intensities could be significantly enhanced since the PET process was inhibited, while the QDs maintained constant intensities. Accordingly, the ratio of the dual-emission intensities of green NBD and red QDs could be utilized for turn-on fluorescent detection of 2,4-D, along with continuous color changes from orange-red to green readily observed by the naked eye. The as-prepared fluorescence sensor obtained high sensitivity with a low detection limit of 0.14μM within 5min, and distinguished recognition selectivity for 2,4-D over its analogs. Moreover, the sensor was successfully applied to determine 2,4-D in real water samples, and high recoveries at three spiking levels of 2,4-D ranged from 95.0% to 110.1% with precisions below 4.5%. The simple, rapid and reliable visual sensing strategy would not only provide potential applications for high selective ultratrace analysis of complicated matrices, but also greatly enrich the research connotations of Molecularly imprinted sensors.
Banshi Dhar Gupta - One of the best experts on this subject based on the ideXlab platform.
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surface plasmon resonance based optical fiber sensor for atrazine detection using Molecular Imprinting technique
Sensors and Actuators B-chemical, 2016Co-Authors: Harshit Agrawal, Anand M. Shrivastav, Banshi Dhar GuptaAbstract:Abstract We reported a successful effort for fabrication and characterization of surface plasmon resonance based fiber optic sensor to detect atrazine by Molecular imprinted technique. The fabrication of sensing probe is done by coating a 40 nm thick silver film over the unclad core of an optical fiber. Further, an over layer of Molecular imprinted (MIP) polymer having atrazine as template molecule has been coated over Ag coated region. Spectral interrogation method has been used for characterization of the probe. A red shift of 38 nm in resonance wavelength has been observed for atrazine concentration range of 0 M–10−7 M. Sensitivity of the sensor has been found to be 17.34 nm/log M for atrazine concentration of 10−12 M. The sensor has lowest detection limit of 1.92 × 10−14 M and quantification limit of 7.61 × 10−14 M. Sensor has been found to be highly selective as well as highly sensitive due to a combined approach of Molecular Imprinting and surface plasmon resonance. Besides them, sensor has numerous advantages such as low cost, fast response, immune to electromagnetic interface and capability of online monitoring and remote sensing.
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Fiber optic profenofos sensor based on surface plasmon resonance technique and Molecular Imprinting.
Biosensors & bioelectronics, 2015Co-Authors: Anand M. Shrivastav, Sruthi P. Usha, Banshi Dhar GuptaAbstract:Abstract A successful approach for the fabrication and characterization of an optical fiber sensor for the detection of profenofos based on surface plasmon resonance (SPR) and Molecular Imprinting is introduced. Molecular Imprinting technology is used for the creation of three dimensional binding sites having complementary shape and size of the specific template molecule over a polymer for the recognition of the same. Binding of template molecule with Molecularly imprinted polymer (MIP) layer results in the change in the dielectric nature of the sensing surface (polymer) and is identified by SPR technique. Spectral interrogation method is used for the characterization of the sensing probe. The operating profenofos concentration range of the sensor is from 10 −4 to 10 −1 µg/L. A red shift of 18.7 nm in resonance wavelength is recorded for this profenofos concentration range. The maximum sensitivity of the sensor is 12.7 nm/log (µg/L) at 10 −4 µg/L profenofos concentration. Limit of detection (LOD) of the sensor is found to be 2.5×10 −6 µg/L. Selectivity measurements predict the probe highly selective for the profenofos molecule. Besides high sensitivity due to SPR technique and selectivity due to Molecular Imprinting, proposed sensor has numerous other advantages like immunity to electromagnetic interference, fast response, low cost and capability of online monitoring and remote sensing of analyte due to the fabrication of the probe on optical fiber.
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Fiber optic SPR sensor for the detection of melamine using Molecular Imprinting
Sensors and Actuators B: Chemical, 2015Co-Authors: Anand M. Shrivastav, Satyendra K Mishra, Banshi Dhar GuptaAbstract:Fabrication and characterization of a surface plasmon resonance based fiber optic sensor for the detection of melamine using Molecular Imprinting are reported. The probe is fabricated by coating a thin film of silver over the unclad core of an optical fiber which is further coated with the Molecular imprinted (MIP) polymer using melamine as template molecule. The MIP layer creates binding sites, complementary of the template molecule on the surface. The template molecules have the capability to bind with these active sites. The performance of the sensor is tested for the melamine concentration range from 10-7M to 10-1M. A shift of 19 nm in resonance wavelength is recorded for this concentration range. The sensitivity is maximized by optimizing melamine concentration in MIP layer formation and the pH of the sample solution. The selectivity of the probe is checked using different analytes and is found to be highly selective for melamine. The sensitivity of the sensor is improved by introducing a thin layer of aluminum between silver and MIP layer. The sensor has advantages of fast response, high selectivity and sensitivity, low cost and can be used for online monitoring and remote sensing applications.
Klaus Mosbach - One of the best experts on this subject based on the ideXlab platform.
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Molecular Imprinting: Synthetic materials as substitutes for biological antibodies and receptors
Chemistry of Materials, 2008Co-Authors: Klaus MosbachAbstract:Molecular Imprinting is a versatile technique providing functional materials able to recognize and in some cases respond to biological and chemical agents of interest. In contrast to biological antibodies, the best known receptors derived from biological combinatorial processes, Molecularly imprinted polymers (MIPs) are obtained by template-directed synthesis. Thus, Molecular Imprinting can more properly be characterized as a "rational design" approach, allowing research and application problems to be solved. Using simple Molecular building blocks, material chemists can now produce tailored synthetic materials of much improved stabilities able to replace or complement natural receptors.
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non covalent Molecular Imprinting with emphasis on its application in separation and drug development
Journal of Molecular Recognition, 2006Co-Authors: Huiqi Zhang, Klaus MosbachAbstract:The Molecular Imprinting technique can be defined as the formation of specific nano-sized cavities by means of template-directed synthesis. The resulting Molecularly imprinted polymers (MIPs), which often have an affinity and a selectivity approaching those of antibody-antigen systems, have thus been coined "artificial antibodies." MIPs are characterized by their high specificity, ease of preparation, and their thermal and chemical stability. They have been widely studied in connection with many potential applications, including their use for separation and isolation purposes, as antibody mimics (biomimetic assays and sensors), as enzyme mimics, in organic synthesis, and in drug delivery. The non-covalent Imprinting approach, developed mainly in Lund, has proven to be more versatile than the alternative covalent approach because of its preparation being less complicated and of the broad selection of functional monomers and possible target molecules that are available. The paper presents a review of studies of this versatile technique in the areas of separation and drug development, with emphasis being placed on work carried out in our laboratory.
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peer reviewed Molecular Imprinting new possibilities for sensor technology
Analytical Chemistry, 1997Co-Authors: Dario Kriz, Olof Ramstrom, Klaus MosbachAbstract:Molecular Imprinting-based biomimetic sensors could provide an alternative to often unstable biosensors for industry, medicine, and environmental analysis
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carbon carbon bond formation using substrate selective catalytic polymers prepared by Molecular Imprinting an artificial class ii aldolase
Journal of Organic Chemistry, 1996Co-Authors: Jun Matsui, Ian A Nicholls, Isao Karube, Klaus MosbachAbstract:Carbon-carbon bond formation using substrate selective catalytic polymers prepared by Molecular Imprinting: an artificial class II aldolase
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the emerging technique of Molecular Imprinting and its future impact on biotechnology
Nature Biotechnology, 1996Co-Authors: Klaus Mosbach, Olof RamstromAbstract:The technique of Molecular Imprinting allows the formation of specific recognition and catalytic sites in macromolecules by the use of templates. Molecularly imprinted polymers have been applied in an increasing number of applications where Molecular binding events are of interest. These include (i) the use of Molecularly imprinted polymers as tailor-made separation materials, (ii) antibody and receptor binding site mimics in recognition and assay systems, (iii) enzyme mimics for catalytic applications, and (iv) recognition elements in bio-sensors. The stability and low cost of Molecularly imprinted polymers make them advantageous for use in analysis as well as in industrial scale production and application.
Anand M. Shrivastav - One of the best experts on this subject based on the ideXlab platform.
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surface plasmon resonance based optical fiber sensor for atrazine detection using Molecular Imprinting technique
Sensors and Actuators B-chemical, 2016Co-Authors: Harshit Agrawal, Anand M. Shrivastav, Banshi Dhar GuptaAbstract:Abstract We reported a successful effort for fabrication and characterization of surface plasmon resonance based fiber optic sensor to detect atrazine by Molecular imprinted technique. The fabrication of sensing probe is done by coating a 40 nm thick silver film over the unclad core of an optical fiber. Further, an over layer of Molecular imprinted (MIP) polymer having atrazine as template molecule has been coated over Ag coated region. Spectral interrogation method has been used for characterization of the probe. A red shift of 38 nm in resonance wavelength has been observed for atrazine concentration range of 0 M–10−7 M. Sensitivity of the sensor has been found to be 17.34 nm/log M for atrazine concentration of 10−12 M. The sensor has lowest detection limit of 1.92 × 10−14 M and quantification limit of 7.61 × 10−14 M. Sensor has been found to be highly selective as well as highly sensitive due to a combined approach of Molecular Imprinting and surface plasmon resonance. Besides them, sensor has numerous advantages such as low cost, fast response, immune to electromagnetic interface and capability of online monitoring and remote sensing.
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Fiber optic profenofos sensor based on surface plasmon resonance technique and Molecular Imprinting.
Biosensors & bioelectronics, 2015Co-Authors: Anand M. Shrivastav, Sruthi P. Usha, Banshi Dhar GuptaAbstract:Abstract A successful approach for the fabrication and characterization of an optical fiber sensor for the detection of profenofos based on surface plasmon resonance (SPR) and Molecular Imprinting is introduced. Molecular Imprinting technology is used for the creation of three dimensional binding sites having complementary shape and size of the specific template molecule over a polymer for the recognition of the same. Binding of template molecule with Molecularly imprinted polymer (MIP) layer results in the change in the dielectric nature of the sensing surface (polymer) and is identified by SPR technique. Spectral interrogation method is used for the characterization of the sensing probe. The operating profenofos concentration range of the sensor is from 10 −4 to 10 −1 µg/L. A red shift of 18.7 nm in resonance wavelength is recorded for this profenofos concentration range. The maximum sensitivity of the sensor is 12.7 nm/log (µg/L) at 10 −4 µg/L profenofos concentration. Limit of detection (LOD) of the sensor is found to be 2.5×10 −6 µg/L. Selectivity measurements predict the probe highly selective for the profenofos molecule. Besides high sensitivity due to SPR technique and selectivity due to Molecular Imprinting, proposed sensor has numerous other advantages like immunity to electromagnetic interference, fast response, low cost and capability of online monitoring and remote sensing of analyte due to the fabrication of the probe on optical fiber.
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Fiber optic SPR sensor for the detection of melamine using Molecular Imprinting
Sensors and Actuators B: Chemical, 2015Co-Authors: Anand M. Shrivastav, Satyendra K Mishra, Banshi Dhar GuptaAbstract:Fabrication and characterization of a surface plasmon resonance based fiber optic sensor for the detection of melamine using Molecular Imprinting are reported. The probe is fabricated by coating a thin film of silver over the unclad core of an optical fiber which is further coated with the Molecular imprinted (MIP) polymer using melamine as template molecule. The MIP layer creates binding sites, complementary of the template molecule on the surface. The template molecules have the capability to bind with these active sites. The performance of the sensor is tested for the melamine concentration range from 10-7M to 10-1M. A shift of 19 nm in resonance wavelength is recorded for this concentration range. The sensitivity is maximized by optimizing melamine concentration in MIP layer formation and the pH of the sample solution. The selectivity of the probe is checked using different analytes and is found to be highly selective for melamine. The sensitivity of the sensor is improved by introducing a thin layer of aluminum between silver and MIP layer. The sensor has advantages of fast response, high selectivity and sensitivity, low cost and can be used for online monitoring and remote sensing applications.
