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Guoqiang Zhu - One of the best experts on this subject based on the ideXlab platform.
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Sole recombinant Muscovy duck parvovirus infection in Muscovy ducklings can form characteristic intestinal embolism.
Veterinary microbiology, 2020Co-Authors: Jianye Wang, Zhixian Wang, Jingyu Jia, Guoqiang ZhuAbstract:Recombinant Muscovy duck parvovirus (rMDPV) has been recently identified as a novel pathogen circulating in Chinese Muscovy duck flocks in the past two decades. Different from classical MDPV, rMDPV infection can form embolism in the intestinal tract of deceased Muscovy ducklings. However, whether rMDPV acts as the sole causative agent involved in the formation of the characteristic embolism in Muscovy ducklings remains unclear. In this study, an infectious plasmid clone pZW containing the complete genome of strain ZW, a previously characterized rMDPV isolate, was constructed, and a single nucleotide mutation was then introduced in the VP1 gene within pZW as the genetic marker. Transfection of pZW in 11-day-old embryonated Muscovy duck eggs via the chorioallantoic membrane route resulted in the rescue of the infectious virus. The rescued virus exhibited similar biological characteristics to its parental strain ZW, as evaluated by the median embryo lethal dose and the replication kinetics in embryonated Muscovy duck eggs. Muscovy duckling infection tests showed that the rescued virus and parental strain can kill all Muscovy ducklings within 7 days post-infection. Postmortem examination revealed that embolism can be observed in the intestinal tracts of deceased ducklings in the rescued and parental virus infection groups. Collectively, the present study demonstrated that sole rMDPV infection of Muscovy ducklings, without participation of other pathogens, is enough to form characteristic embolism in the intestinal tract.
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Transfection of embryonated Muscovy duck eggs with a recombinant plasmid is suitable for rescue of infectious Muscovy duck parvovirus.
Archives of virology, 2017Co-Authors: Jianye Wang, Yu Huang, Jueyi Ling, Zhixiang Wang, Guoqiang ZhuAbstract:For members of the family Parvoviridae, rescue of infectious virus from recombinant plasmid is usually done in cultured cells. In this study, the whole genome of the pathogenic Muscovy duck parvovirus (MDPV) strain YY was cloned into the pBluescript II (SK) vector, generating recombinant plasmid pYY. With the aid of a transfection reagent, pYY plasmid was inoculated into 11-day-old embryonated Muscovy duck eggs via the chorioallantoic membrane route, resulting in the successful rescue of infectious virus and death of the embryos. The rescued virus exhibited pathogenicity in Muscovy ducklings similar to that of its parental strain, as evaluated based on the mortality rate. The results demonstrate that plasmid transfection in embryonated Muscovy duck eggs is a convenient and efficacious method for rescue of infectious MDPV in comparison to transfection of primary cells, which is somewhat time-consuming and laborious.
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construction and sequencing of an infectious clone of the goose embryo adapted Muscovy duck parvovirus vaccine strain fz91 30
Virology Journal, 2016Co-Authors: Jianye Wang, Yu Huang, Mingxu Zhou, Philip R Hardwidge, Guoqiang ZhuAbstract:Background Muscovy duck parvovirus (MDPV) is the etiological agent of Muscovy duckling parvoviral disease, which is characterized by diarrhea, locomotive dysfunction, stunting, and death in young ducklings, and causes substantial economic losses in the Muscovy duck industry worldwide. FZ91-30 is an attenuated vaccine strain that is safe and immunogenic to ducklings, but the genomic information and molecular mechanism underlining the attenuation are not understood.
Jianye Wang - One of the best experts on this subject based on the ideXlab platform.
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Sole recombinant Muscovy duck parvovirus infection in Muscovy ducklings can form characteristic intestinal embolism.
Veterinary microbiology, 2020Co-Authors: Jianye Wang, Zhixian Wang, Jingyu Jia, Guoqiang ZhuAbstract:Recombinant Muscovy duck parvovirus (rMDPV) has been recently identified as a novel pathogen circulating in Chinese Muscovy duck flocks in the past two decades. Different from classical MDPV, rMDPV infection can form embolism in the intestinal tract of deceased Muscovy ducklings. However, whether rMDPV acts as the sole causative agent involved in the formation of the characteristic embolism in Muscovy ducklings remains unclear. In this study, an infectious plasmid clone pZW containing the complete genome of strain ZW, a previously characterized rMDPV isolate, was constructed, and a single nucleotide mutation was then introduced in the VP1 gene within pZW as the genetic marker. Transfection of pZW in 11-day-old embryonated Muscovy duck eggs via the chorioallantoic membrane route resulted in the rescue of the infectious virus. The rescued virus exhibited similar biological characteristics to its parental strain ZW, as evaluated by the median embryo lethal dose and the replication kinetics in embryonated Muscovy duck eggs. Muscovy duckling infection tests showed that the rescued virus and parental strain can kill all Muscovy ducklings within 7 days post-infection. Postmortem examination revealed that embolism can be observed in the intestinal tracts of deceased ducklings in the rescued and parental virus infection groups. Collectively, the present study demonstrated that sole rMDPV infection of Muscovy ducklings, without participation of other pathogens, is enough to form characteristic embolism in the intestinal tract.
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Transfection of embryonated Muscovy duck eggs with a recombinant plasmid is suitable for rescue of infectious Muscovy duck parvovirus.
Archives of virology, 2017Co-Authors: Jianye Wang, Yu Huang, Jueyi Ling, Zhixiang Wang, Guoqiang ZhuAbstract:For members of the family Parvoviridae, rescue of infectious virus from recombinant plasmid is usually done in cultured cells. In this study, the whole genome of the pathogenic Muscovy duck parvovirus (MDPV) strain YY was cloned into the pBluescript II (SK) vector, generating recombinant plasmid pYY. With the aid of a transfection reagent, pYY plasmid was inoculated into 11-day-old embryonated Muscovy duck eggs via the chorioallantoic membrane route, resulting in the successful rescue of infectious virus and death of the embryos. The rescued virus exhibited pathogenicity in Muscovy ducklings similar to that of its parental strain, as evaluated based on the mortality rate. The results demonstrate that plasmid transfection in embryonated Muscovy duck eggs is a convenient and efficacious method for rescue of infectious MDPV in comparison to transfection of primary cells, which is somewhat time-consuming and laborious.
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construction and sequencing of an infectious clone of the goose embryo adapted Muscovy duck parvovirus vaccine strain fz91 30
Virology Journal, 2016Co-Authors: Jianye Wang, Yu Huang, Mingxu Zhou, Philip R Hardwidge, Guoqiang ZhuAbstract:Background Muscovy duck parvovirus (MDPV) is the etiological agent of Muscovy duckling parvoviral disease, which is characterized by diarrhea, locomotive dysfunction, stunting, and death in young ducklings, and causes substantial economic losses in the Muscovy duck industry worldwide. FZ91-30 is an attenuated vaccine strain that is safe and immunogenic to ducklings, but the genomic information and molecular mechanism underlining the attenuation are not understood.
Lin Fengqiang - One of the best experts on this subject based on the ideXlab platform.
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Muscovy Duck Reovirus Infection Disrupts the Composition of Intestinal Microbiota in Muscovy Ducklings.
Current microbiology, 2020Co-Authors: Xiuqin Chen, Lin Fengqiang, Chen Shaoying, Min Zheng, Meiqing Huang, Xiao Shifeng, Shi-long ChenAbstract:Muscovy duck reovirus (MDRV) is highly pathogenic to young Muscovy ducklings. Although MDRV infection results in ducklings’ acute watery diarrhea, the effect of MDRV infection on the composition of host’s intestinal microbiota remains poorly understood. This study was conducted to investigate the impacts of MDRV on the composition of Muscovy ducklings’ intestinal bacterial community. Three-day-old Muscovy ducklings were inoculated with either the virulent MDRV strain MW9710 or sterile Hank's solution, respectively. The cecal microbiota was analyzed between control and mock MDRV-infected ducklings using Illumina MiSeq sequencing at 6 dpi and 17 dpi, respectively. The results indicated that MDRV infection damaged the intestinal mucosa. In addition, MDRV infection caused severe perturbations of gut microbiota by decreasing microbial richness, altering the abundance of certain genera of the gut microbiota at 6 dpi. Specifically, the relative abundance of short chain fatty acids-producing bacteria (including Shuttleworthia, Streptococcus, and Ruminococcus) was reduced in MDRV-infected ducklings than those of control group, whereas, with an enrichment of Enterobacteriaceae (including Plesiomonas, Escherichia_Shigella and Proteus). Furthermore, microbiota analysis showed that the gut microbiota dysbiosis caused by MDRV infection was basically recovered at 17 dpi. Collectively, this study demonstrated that the gut microbiota of Muscovy ducklings were altered due to MDRV infection, mainly featuring as a net loss of beneficial bacteria and a compensatory proliferation of pathogenic bacteria, which may lead to severe pathology to the intestinal mucosa, and ultimately acute diarrhea. These results will provide insights into the pathology of MDRV infection.
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Development of a live attenuated vaccine against Muscovy duck reovirus infection.
Vaccine, 2018Co-Authors: Shi-long Chen, Lin Fengqiang, Chen Shaoying, Zhu Xiaoli, Cheng Xiaoxia, Shao Wang, Hu Qilin, Bin Jiang, Min Zheng, Meiqing HuangAbstract:Abstract The Muscovy duck reovirus (MDRV) is a highly pathogenic virus that causes substantial economic losses in the Muscovy duck industry. While MDRV poses a significant threat to Muscovy ducklings, no vaccine candidates are available to date to alleviate MDRV infection throughout the world. The present study presents efforts toward establishing an attenuated vaccine for MDRV. For this purpose, a live attenuated vaccine strain named CA was obtained via alternate propagation of the MDRV isolate MW9710 in both Muscovy duck embryo fibroblasts (MDEFs) and chicken embryo fibroblasts (CEFs) for 90 passages. The CA strain achieved an adaptive growth capacity in CEFs with a viral titer that ranged between 105.0–105.5 TCID50/100 μL and lost its pathogenicity in 1-day-old Muscovy ducklings. Compared to the parent strain MW9710, the CA strain has 42 scattered amino acid substitutions, most of which are located in the λB, λC, μB, σB, and σC protein. The CA strain maintained its attenuation and showed no gene mutation or virulence reversion after back propagation into 1-day-old ducklings for five rounds. The minimum protective dose was calculated to be 300 TCID50 of the CA strain. Furthermore, a single dose of CA vaccine protected immunized ducklings against lethal challenge by the virulent MDRV strain MW9710 and significantly decreased viral loads. In summary, the CA strain exhibited striking genetic stability, excellent safety, and effective immunogenicity. This CA strain of MDRV is a promising vaccine candidate for the prevention and control of MDRV infection.
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Genetic Characterization of a Potentially Novel Goose Parvovirus Circulating in Muscovy Duck Flocks in Fujian Province, China
The Journal of veterinary medical science, 2013Co-Authors: Shao Wang, Chen Shaoying, Zhu Xiaoli, Cheng Xiaoxia, Shi-long Chen, Lin FengqiangAbstract:We report a novel goose parvovirus (MDGPV/PT) isolated from an affected Muscovy duck in Fujian Province, China. In this study, the NS1 sequence analyses indicated a close genetic relationship between MDGPV/PT and Muscovy duck parvovirus (MDPV) strains, although MDGPV/DY, which was isolated from a Muscovy duck in 2006 in Sichuan Province, could be divided into GPV-related groups. Phylogenetic analysis showed that except for differences in the NS1 gene, MDGPV strains PT and DY are closely related to a parvovirus that infects domestic waterfowls. This is the first demonstration of recombination between goose and Muscovy duck parvoviruses in nature, and MDGPV/PT might have led to the generation of a novel waterfowl parvovirus strain circulating in Muscovy duck flocks in China.
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Influence of different infective dose of MDRV on immune responses and cytotoxic activity in Muscovy ducks
Chinese journal of veterinary science, 2010Co-Authors: Lin Fengqiang, Wang Shao, Chen Shilong, Chen Shaoying, Zhu Xiaoli, Cheng Xiaoxia, Gao Chunliang, Li ZhaolongAbstract:To access the effect of different infective dose of MDRV(Muscovy duck reovirus) on the immune response in Muscovy ducks,immune responses of the lymphocytes in the blood to ConA and LPS,cytotoxic activity of cytotoxic T lymphocytes(CTL) and natural killer cells were detected.The results showed that MDRV infection,not only at higher dose but at low dose,could cause the immunosuppression of lymphocytes proliferation response to ConA and LPS in Muscovy ducks.The cytotoxic activities of NK cel1 and CTL were reduced in Muscovy ducks infected with MDRV.And Muscovy ducks infected with MDRV were slow-growth,splenomegaly,thymus and bursa atrophy.So MDRV infection appeared to depress certain immune response,that was related to the infection dose.
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Detection of Muscovy duck reovirus by RT-PCR
Chinese journal of veterinary science, 2004Co-Authors: Hu Qilin, Chen Shao-ying, Lin Fengqiang, Chen Shilong, Zhu Xiaoli, Cheng Xiaoxia, Lin Tianlong, Jiang Bin, Li Yi-ying, Cheng YuquanAbstract:A pair of primers HP11, HP12 were designed according to the nucleatide sequence of S1 gene of Muscovy duck reovirus(MDRV) in GenBank to amplify the S1 gene of MDRV from 4 MDRV strains MDRV-MW9710, MW9806, MW9809 and MW9810 and avian reovirus(ARV) S1133 strain and Muscovy duck embryo fibroblast(MDEF) cell cultures. Only one MDRV-specific 300 bp cDNA products were amplified from all the 4 Muscovy duck reovirus, but not from ARV S1133 strain and MDEF cell cultures. The detectable viral dsRNA amount of this RT-PCR was 0.01 pg. The results of 3 individual times were consistant. These results indicate that this RT-PCR can be used for rapid detection of the Muscovy duck reovirus disease.
Marie Dominique Bernadet - One of the best experts on this subject based on the ideXlab platform.
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Influence of grand-mother diet on offspring performances through the male line in Muscovy duck
BMC genetics, 2015Co-Authors: J. M. Brun, Stéphane Davail, Marie Dominique Bernadet, A. Cornuez, Sophie Leroux, Loys Bodin, Benjamin Basso, Mathilde Jaglin, Michel Lessire, X. MartinAbstract:Background In mammals, multigenerational environmental effects have been documented by either epidemiological studies in human or animal experiments in rodents. Whether such phenomena also occur in birds for more than one generation is still an open question. The objective of this study was to investigate if a methionine deficiency experienced by a mother (G0) could affect her grand-offspring phenotypes (G2 hybrid mule ducks and G2 purebred Muscovy ducks), through their Muscovy sons (G1). Muscovy drakes are used for the production of mule ducks, which are sterile offspring of female common duck (Anas platyrhynchos) and Muscovy drakes (Cairina moschata). In France, mule ducks are bred mainly for the production of “foie gras”, which stems from hepatic steatosis under two weeks of force-feeding (FF). Two groups of female Muscovy ducks received either a methionine deficient diet or a control diet. Their sons were mated to Muscovy or to common duck females to produce Muscovy or Mule ducks, respectively. Several traits were measured in the G2 progenies, concerning growth, feed efficiency during FF, body composition after FF, and quality of foie gras and magret.
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Fractional apoB-VLDL hepatic synthesis rate in Muscovy and Pekin ducks fed ad libitum
Archiv für Geflügelkunde Archives de Science Avicole Archives of Poultry Science, 2012Co-Authors: Gladys Saez, Elisabeth Baeza, Stéphane Davail, Marie Dominique Bernadet, Isabelle Savary-auzeloux, Delphine Durand, Dominique Bauchart, Dominique GruffatAbstract:Muscovy duck has a higher ability to develop hepatic steatosis than Pekin duck. This could result from a lower ability of liver to secrete lipids and lipoproteins. To test this hypothesis, we measured the liver apoB-VLDL secretion in Muscovy and Pekin ducks ad libitum -fed by injecting a flooding dose of [1-13C]-valine. In a first experiment, we found that the delay required for the secretion of apoB by the liver was similar for Muscovy and Pekin ducks (25 min, approximatively). In a second study, the liver ability for apoB synthesis and secretion in both species was assessed in vivo 55 min after the injection of the flooding dose of [1-13C]-valine. The fractional apoB-VLDL synthesis rateswas similar for Muscovy and Pekin ducks ( P = 0.449). However, TG concentration measured in plasma VLDL 3 h after the last meal was higher in Pekin ducks than in Muscovy ducks. Pekin duck seemed to limit the accumulation of lipids in liver by increasing the TG content in VLDL withoutincreasing apoB secretion. Consequently, plasma lipids couldparticipate to the higher lipid deposition in adipose and muscle tissues. However, the concentration of TG-VLDL in plasma results from a balance between liver secretion, peripheral tissue uptake and liver uptake. These last mechanisms require further investigation.
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genetic parameters of product quality and hepatic metabolism in fattened mule ducks
Journal of Animal Science, 2011Co-Authors: C Marieetancelin, Elisabeth Baeza, Stéphane Davail, Marie Dominique Bernadet, Benjamin Basso, Karine Gontier, X Fernandez, Zulma G Vitezica, Denis Bastianelli, Jeanpaul BrunAbstract:Genetic parameters of traits related to hepatic lipid metabolism, carcass composition, and product quality of overfed mule ducks were estimated on both parental lines of this hybrid: the common duck line for the maternal side and the Muscovy line for the paternal side. The originality of the statistical model was to include simultaneously the additive genetic effect of the common ducks and that of the Muscovy ducks, revealing a greater genetic determinism in common than in Muscovy. Plasma metabolic indicators (glucose, triglyceride, and cholesterol contents) were heritable, in particular at the end of the overfeeding period, and heritabilities increased with the overfeeding stage. Carcass composition traits were highly heritable in the common line, with values ranging from 0.15 for liver weight, 0.21 for carcass weight, and 0.25 for abdominal fat weight to 0.32 for breast muscle weight. Heritabilities of technological outputs were greater for the fatty liver (0.19 and 0.08, respectively, on common and Muscovy sides for liver melting rate) than for the pectoralis major muscle (between 0.02 and 0.05 on both parental sides for cooking losses). Fortunately, the processing industry is mainly facing problems in liver quality, such as too high of a melting rate, than in meat quality. The meat quality appraisal criteria (such as texture and cooking losses), usually dependent on pH and the rate of decline of pH, were also very lowly heritable. This study demonstrated that genetic determinism of meat quality and ability of overfeeding is not similar in the common population and in the Muscovy population; traits related to fattening, muscle development, and BW have heritability values from 2 to 4 times greater on the common line than on the Muscovy line, which is relevant for considering different selection strategies. (Resume d'auteur)
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Genetic parameters of product's quality and hepatic metabolism in fattened mule ducks
Journal of Animal Science, 2011Co-Authors: Christel Marie-etancelin, Elisabeth Baeza, Stéphane Davail, Marie Dominique Bernadet, Benjamin Basso, Karine Gontier, X Fernandez, Zulma G Vitezica, Denis Bastianelli, Gerard GuyAbstract:Genetic parameters of traits related to hepatic lipid metabolism, carcass composition and products quality of overfed mule ducks were estimated on both parental lines of this hybrid: the “common duck” line for the maternal side and the “Muscovy” line for the paternal side. The originality of the statistical model was to include simultaneously the additive genetic effect of the common ducks and that of the Muscovy ducks, revealing a higher genetic determinism in common than in Muscovy. Plasma metabolic indicators (glucose, triglycerides and cholesterol contents) were heritable, in particular at the end of the overfeeding period, and heritabilities increased with the overfeeding stage. Carcass composition traits were highly heritable in the common line, with values ranging from 0.15 for liver weight, 0.21 for carcass weight and 0.25 for abdominal fat weight to 0.32 for breast muscle weight. Heritabilities of technological outputs were higher for the fatty liver (0.19 and 0.08 respectively on common and Muscovy sides for liver melting rate) than for the Pectoralis major muscle (between 0.02 and 0.05 on both parental sides for cooking losses). Fortunately, the processing industry is mainly facing problems in liver quality –such as too high melting rate- than in meat quality. The meat quality appraisal criteria such as texture and cooking losses), usually dependent on the pH and the falling speed of the pH, were also very lowly heritable. This study demonstrated that genetic determinism of meat quality and ability of over-feeding is not similar in the common population and in the Muscovy population: traits related to fattening, muscle development and body weights have heritabilities values from 2 to 4 times higher on the common line than on the Muscovy line, which is relevant for considering different selection strategies.
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influence of lipoprotein lipase activity on plasma triacylglycerol concentration and lipid storage in three genotypes of ducks
Comparative Biochemistry and Physiology A-molecular & Integrative Physiology, 2007Co-Authors: Jean-marc Andre, K Gontierlatonnelle, Marie Dominique Bernadet, B Davail, R Hooparis, Stéphane DavailAbstract:Abstract The lipoprotein-lipase (LPL) hydrolyses the triacylglycerols (TG) secreted by the liver and, thus, allows the storage of lipids onto the extrahepatic tissues. The LPL activity has been studied by injection of LPL antibodies in three genotypes of ducks (Muscovy ( Cairina moschat ), Pekin ( Anas plathyrhynchos ) and Mule (hybrids of male Muscovy ducks and female Pekin ducks)) under overfeeding condition. The results show a similar weight gain between injected and control animals. A higher liver steatosis is observed in Mule ducks (616 ± 18 g; 8.79% of body mass (BW)) and Muscovy ducks (514 ± 13 g; 7.05% BW) compared to Pekin ducks (353 ± 21 g; 5.89% BW, p p p
Feng Chen - One of the best experts on this subject based on the ideXlab platform.
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Isolation and characterization of a recombinant Muscovy duck parvovirus circulating in Muscovy ducks in South China.
Archives of virology, 2020Co-Authors: Hanqin Shen, Jianfei Huang, Zhuanqiang Yan, Lijuan Yin, Qingfeng Zhou, Feng ChenAbstract:In 2019, flocks of Muscovy ducks presented with clinical signs typical of MDPV infection. The MDPV GD201911 strain was isolated by inoculating samples from positive birds into Muscovy duck embryos. Challenge with the isolate GD201911 caused typical MDPV disease symptoms and resulted in 25%-40% mortality, depending on the challenge dose, indicating the high pathogenicity of GD201911 for Muscovy ducks. Genome sequencing and phylogenetic analysis demonstrated that GD201911 clustered with recombinant MDPV strains, indicating that recombinant MDPV is circulating in China. Epidemiological monitoring should be performed continuously to assist with decision making for disease control.
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Isolation and characterization of a subtype C avian metapneumovirus circulating in Muscovy ducks in China
Veterinary Research, 2014Co-Authors: Shikai Sun, Feng Chen, Sheng Cao, Jiajia Liu, Wen Lei, Guangwei Li, Yongfeng Song, Junpeng Lu, Chuang Liu, Jianping QinAbstract:Subtype C avian metapneumovirus (aMPV-C), is an important pathogen that can cause egg-drop and acute respiratory diseases in poultry. To date, aMPV-C infection has not been documented in Muscovy ducks in China. Here, we isolated and characterized an aMPV-C, designated S-01, which has caused severe respiratory disease and noticeable egg drop in Muscovy duck flocks in south China since 2010. Electron microscopy showed that the isolate was an enveloped virus exhibiting multiple morphologies with a diameter of 20–500 nm. The S-01 strain was able to produce a typical cytopathic effect (CPE) on Vero cells and cause death in 10- to 11-day-old Muscovy duck embryos. In vivo infection of layer Muscovy ducks with the isolate resulted in typical clinical signs and pathological lesions similar to those seen in the original infected cases. We report the first complete genomic sequence of aMPV-C from Muscovy ducks. A phylogenetic analysis strongly suggested that the S-01 virus belongs to the aMPV-C family, sharing 92.3%-94.3% of nucleotide identity with that of aMPV-C, and was most closely related to the aMPV-C strains isolated from Muscovy ducks in France. The deduced eight main proteins (N, P, M, F, M2, SH, G and L) of the novel isolate shared higher identity with hMPV than with other aMPV (subtypes A, B and D). S-01 could bind a monoclonal antibody against the F protein of hMPV. Together, our results indicate that subtype-C aMPV has been circulating in Muscovy duck flocks in South China, and it is urgent for companies to develop new vaccines to control the spread of the virus in China.
