The Experts below are selected from a list of 321 Experts worldwide ranked by ideXlab platform
Shunsuke Ishii - One of the best experts on this subject based on the ideXlab platform.
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Trans-activation by the Drosophila MYB Gene product requires a Drosophila homologue of CBP
FEBS letters, 1997Co-Authors: De-xing Hou, Hiroshi Akimaru, Shunsuke IshiiAbstract:Attempts to demonstrate trans-activation activity by the Drosophila MYB Gene product (D-MYB) have been unsuccessful so far. We demonstrate that co-transfection of Schneider cells with a plasmid expressing the Drosophila homologue of transcriptional co-activator CBP (dCBP) results in trans-activation by D-MYB. Using this assay system, the functional domains of D-MYB were analyzed. Two domains located in the N-proximal region, one of which is required for DNA binding and the other for dCBP binding, are both necessary and sufficient for trans-activation. In this respect, D-MYB is similar to c-MYB and A-MYB, but different from mammalian B-MYB. These results shed light on how the MYB Gene diverged during the course of evolution.
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Structure and function of the proteins encoded by the MYB Gene family.
Current topics in microbiology and immunology, 1996Co-Authors: Chie Kanei-ishii, Teruaki Nomura, Kazuhiro Ogata, Akinori Sarai, Takashi Yasukawa, Shigeki Tashiro, T. Takahashi, Y. Tanaka, Shunsuke IshiiAbstract:The nuclear proto-oncoGene c-MYB is the cellular homologue of the v-MYB Gene carried by the chicken leukemia viruses avian myelobastosis virus (AMV) and E26, which transform avian myeloid cells in vitro and in vivo (for review, see ref. 1). c-MYB expression is linked to the differentiation state of the cell, since expression is down-regulated during terminal differentiation of hemopoietic cells (2) and constitutive expression of introduced c-MYB blocks the induced differentiation of erythroleukemia (3). In addition, antisense oligonucleotides to c-MYB appear to impede in vitro hematopoiesis (4) and homozygous c-MYB mutant mice displayed a specific failure of fetal hepatic hematopoiesis (5). These results all indicate a role for c-MYB in maintaining the proliferative state of hematopoietic progenitor cells. Both c-MYB and v-MYB are transcriptional activators (6–9). The v-MYB proteins (v-MYB) encoded by AMV and E26 are amino (N)- and carboxyl (C)-terminally truncated versions of c-MYB. In this report, we shall address the structure and function of each functional domain in c-MYB, and also the relationship between the retroviral-transforming v-MYB Genes and its cellular homologue, the c-MYB Gene, to ask whether changes in the control of transcription may account for the Generation of the transformation phenotype.
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Cell type-specific trans-activation by the B-MYB Gene product: requirement of the putative cofactor binding to the C-terminal conserved domain.
Oncogene, 1995Co-Authors: Shigeki Tashiro, Hiroshi Handa, Takemoto Y, Shunsuke IshiiAbstract:The MYB Gene family has three members, c-MYB, A-MYB and B-MYB. We have examined the trans-activating capacity of the B-MYB Gene product (B-MYB) in various types of cells. B-MYB functions as a transcriptional activator in CV-1 and HeLa cells, but not in NIH3T3 cells, indicating that B-MYB is a cell type-specific transcriptional activator. Deletion analyses of B-MYB have demonstrated that the region conserved between three members of the MYB Gene family (CR for conserved region) is necessary for trans-activation by B-MYB. An in vivo competition assay suggests that regulatory factor(s) that binds to the CR of B-MYB is required for transactivation. Analyses using an affinity resin show that multiple proteins bind to the CR of B-MYB and that the CR-binding proteins in CV-1 and HeLa cells are different from those in NIH3T3 cells. These results suggest that the CR-binding cofactor(s) is critical for the cell type-specific trans-activation by B-MYB.
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Human A-MYB Gene encodes a transcriptional activator containing the negative regulatory domains
FEBS letters, 1995Co-Authors: Tomomi Takahashi, Hideki Nakagoshi, Akinori Sarai, Nobuo Nomura, Tadashi Yamamoto, Shunsuke IshiiAbstract:The MYB Gene family has three members, c-MYB, A-MYB, and B-MYB. A-MYB mRNA is mainly expressed in testis and peripheral blood leukocytes. A-MYB can activate transcription from the promoter containing MYB-binding sites in all cells examined. In addition to the two domains (a DNA-binding domain and a transcriptional activation domain), two negative regulatory domains have been identified in A-MYB. These results indicate that A-MYB functions as a transcriptional activator mainly in testis and peripheral blood cells, and the regulatory mechanism of A-MYB activity is similar to that of c-MYB.
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Multiple nuclear localization signals of the B-MYB Gene product.
FEBS letters, 1994Co-Authors: Yoshihiro Takemoto, Shigeki Tashiro, Hiroshi Handa, Shunsuke IshiiAbstract:Nuclear entry of the B-MYB Gene product (B-MYB) is dependent on multiple nuclear localization signals (NLS's). MutaGenesis of the putative NLS's of B-MYB has identified two separate NLS's, NLS1 and NLS2. Each of the two NLS's is essential for efficient nuclear targeting. NLS2 contains two interdependent basic domains separated by 8 intervening spacer amino acids, and both basic domains are required for nuclear entry. Thus, NLS2 belongs to a class of bipartite NLS's. Like the NLS's in yeast transcription factor SW15, NLS2 contains a putative cdc2 kinase site. However, unlike the case of SWI5, phosphorylation at this site did not affect the nuclear targeting of B-MYB.
Gu Yuan - One of the best experts on this subject based on the ideXlab platform.
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Exploration of G-quadruplex function in c-MYB Gene and its transcriptional regulation by topotecan
International journal of biological macromolecules, 2017Co-Authors: Jiang Zhou, Gu YuanAbstract:Our bioinformatics research shows that there are four G-rich sequences (S1-S4) in the upstream region of the transcription start site of c-MYB Gene, and we have proved that these sequences have the ability to form G-quadruplex structures. This work mainly focuses on G-quadruplex function, recognition and transcription regulation in c-MYB Gene, revealing a novel regulatory element in c-MYB proximal promoter region, and its transcription regulation by G-quadruplex binder. The research has identified that the enhancer effect in c-MYB transcription was primarily affected by the G-quadruplex formed by S1 sequence, and the up-regulation effect may due to the removal of repressive progress of MZF-1 by stabilizing G-quadruplex. Attentions were being paid to the development of G-quadruplex binders for selective recognition, and topotecan was found to have high binding affinity in vitro and could effectively affect the c-MYB transcription activities in cells. The regulation of G-quadruplex with binders in transcriptional, translational levels by Q-RT-PCR and western blot was in expectation of providing a strategy for Gene expression modulation. In conclusion, our study revealed a G-quadruplex structure in c-MYB proximal promoter region, which was of great importance in the regulation of c-MYB function.
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exploration of binding affinity and selectivity of brucine with g quadruplex in the c MYB proto oncoGene by electrospray ionization mass spectrometry
Rapid Communications in Mass Spectrometry, 2016Co-Authors: Jiang Zhou, Jinhui Hai, Gu YuanAbstract:RATIONALE The c-MYB Gene is a potential therapeutic target for human tumors and leukemias. Active ingredients from natural products may be used as drugs in chemotherapy for human cancers. Here, electrospray ionization mass spectrometry (ESI-MS) was used to probe the formation and recognition of the G-quadruplex structure from the G-rich sequence that is found in the c-MYB Gene promoter, 5'-GGGCTGGGCTGGGCGGGG-3'. The aim of our study is to evaluate a potential binder for the c-MYB Gene from natural products, and thereby to modulate c-MYB Gene expression. METHODS ESI-MS, as an effective method, was utilized not only to characterize the formation of the G-quadruplex in the c-MYB oncoGene, but also as a tool to probe the binding characteristics of alkaloid molecules with the target G-quadruplex DNA. RESULTS ESI-MS results with the support of circular dichroism (CD) spectra demonstrated the formation of an intramolecular parallel-stranded G-quadruplex in the c-MYB oncoGene promoter. A screening of six alkaloid molecules showed that brucine (P1) had a strong binding affinity to the c-MYB G-quadruplex DNA. It is notable that P1 can bind selectively to the c-MYB G-quadruplex with respect to duplex DNAs, as well as to G-quadruplexes in other types of Gene sequences. According to ESI-MS results, in which the stability was tested by capillary heating and collision-induced dissociation, the binding of P1 could thermally stabilize the c-MYB G-quadruplex DNA. CONCLUSIONS In this work, brucine (P1), an alkaloid molecule, has been found to bind to the intramolecular parallel G-quadruplex in the c-MYB oncoGene promoter with high affinity and selectivity, and could thermally stabilize the c-MYB G-quadruplex DNA, indicating that the binding of P1 has the potential to modulate c-MYB Gene expression. Copyright © 2015 John Wiley & Sons, Ltd.
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Exploration of binding affinity and selectivity of brucine with G-quadruplex in the c-MYB proto-oncoGene by electrospray ionization mass spectrometry
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2016Co-Authors: Li Huihui, Hai Jinhui, Zhou Jiang, Gu YuanAbstract:RATIONALE: The c-MYB Gene is a potential therapeutic target for human tumors and leukemias. Active ingredients from natural products may be used as drugs in chemotherapy for human cancers. Here, electrospray ionization mass spectrometry (ESI-MS) was used to probe the formation and recognition of the G-quadruplex structure from the G-rich sequence that is found in the c-MYB Gene promoter, 5'-GGGCTGGGCTGGGCGGGG-3'. The aim of our study is to evaluate a potential binder for the c-MYB Gene from natural products, and thereby to modulate c-MYB Gene expression. METHODS: ESI-MS, as an effective method, was utilized not only to characterize the formation of the G-quadruplex in the c-MYB oncoGene, but also as a tool to probe the binding characteristics of alkaloid moleculeswith the target G-quadruplexDNA. RESULTS: ESI-MS results with the support of circular dichroism (CD) spectra demonstrated the formation of an intramolecular parallel-stranded G-quadruplex in the c-MYB oncoGene promoter. A screening of six alkaloid molecules showed that brucine (P1) had a strong binding affinity to the c-MYB G-quadruplex DNA. It is notable that P1 can bind selectively to the c-MYB G-quadruplex with respect to duplex DNAs, as well as to G-quadruplexes in other types of Gene sequences. According to ESI-MS results, in which the stability was tested by capillary heating and collision-induced dissociation, the binding of P1 could thermally stabilize the c-MYB G-quadruplex DNA. CONCLUSIONS: In this work, brucine (P1), an alkaloid molecule, has been found to bind to the intramolecular parallel G-quadruplex in the c-MYB oncoGene promoter with high affinity and selectivity, and could thermally stabilize the c-MYB G-quadruplex DNA, indicating that the binding of P1 has the potential to modulate c-MYB Gene expression. Copyright (C) 2015 John Wiley & Sons, Ltd.National Natural Science Foundation of China [21372021]; 973 Program [2012CB720600, 2012CB720601]; Natural Science Foundation of the Higher Education Institutions of Jiangsu Province [15KJB150017]; Beijing National Laboratory for Molecular Sciences [20140167]; State Key Laboratory of Analytical Chemistry for Life Science [SKLACLS1413]; Priority Academic Program Development of Jiangsu Higher Education InstitutionSCI(E)ARTICLEhuihuili@njnu.edu.cn; guyuan@pku.edu.cn3407-4143
Ralf Stracke - One of the best experts on this subject based on the ideXlab platform.
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The R2R3-MYB Gene family in banana (Musa acuminata): Genome-wide identification, classification and expression patterns.
PloS one, 2020Co-Authors: Boas Pucker, Ashutosh Pandey, Bernd Weisshaar, Ralf StrackeAbstract:The R2R3-MYB Genes comprise one of the largest transcription factor Gene families in plants, playing regulatory roles in plant-specific developmental processes, defense responses and metabolite accumulation. To date MYB family Genes have not yet been comprehensively identified in the major staple fruit crop banana. In this study, we present a comprehensive, genome-wide analysis of the MYB Genes from Musa acuminata DH-Pahang (A genome). A total of 285 R2R3-MYB Genes as well as Genes encoding three other classes of MYB proteins containing multiple MYB repeats were identified and characterised with respect to structure and chromosomal organisation. Organ- and development-specific expression patterns were determined from RNA-Seq data. For 280 M. acuminata MYB Genes for which expression was found in at least one of the analysed samples, a variety of expression patterns were detected. The M. acuminata R2R3-MYB Genes were functionally categorised, leading to the identification of seven clades containing only M. acuminata R2R3-MYBs. The encoded proteins may have specialised functions that were acquired or expanded in Musa during genome evolution. This functional classification and expression analysis of the MYB Gene family in banana establishes a solid foundation for future comprehensive functional analysis of MaMYBs and can be utilized in banana improvement programmes.
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The R2R3-MYB Gene family in banana (Musa acuminata): genome-wide identification, classification and expression patterns
2020Co-Authors: Boas Pucker, Ashutosh Pandey, Bernd Weisshaar, Ralf StrackeAbstract:The R2R3-MYB Genes comprise one of the largest transcription factor Gene families in plants, playing regulatory roles in plant-specific developmental processes, defense responses and metabolite accumulation. To date MYB family Genes have not yet been comprehensively identified in the major staple fruit crop banana. In this study, we present a comprehensive, genome-wide analysis of the MYB Genes from Musa acuminata DH-Pahang (A genome). A total of 286 R2R3-MYB Genes as well as Genes encoding three other classes of MYB proteins containing multiple MYB repeats were identified and characterised with respect to structure and chromosomal organisation. Organ- an delopment-specific expression patterns were determined from RNA-seq data. For 279 M. acuminata MYB Genes for which expression was found in at least one of the analysed samples, a variety of expression patterns were detected. The M. acuminata R2R3-MYB Genes were functionally categorised which led to the identification of seven clades containing only M. acuminata R2R3 MYBs. The encoded proteins may have specialised functions that were acquired or expanded in Musa during genome evolution. The presented functional classification and expression analyses of the MYB Gene family in banana establish a solid foundation for future comprehensive functional analysis of MaMYBs and can be utilized in banana improvement programmes.
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the r2r3 MYB Gene family in arabidopsis thaliana
Current Opinion in Plant Biology, 2001Co-Authors: Ralf Stracke, Martin Werber, Bernd WeisshaarAbstract:MYB factors represent a family of proteins that include the conserved MYB DNA-binding domain. In contrast to animals, plants contain a MYB-protein subfamily that is characterised by the R2R3-type MYB domain. ‘Classical’ MYB factors, which are related to c-MYB, seem to be involved in the control of the cell cycle in animals, plants and other higher eukaryotes. Systematic screens for knockout mutations in MYB Genes, followed by phenotypic analyses and the dissection of mutants with interesting phenotypes, have started to unravel the functions of the 125 R2R3-MYB Genes in Arabidopsis thaliana. R2R3-type MYB Genes control many aspects of plant secondary metabolism, as well as the identity and fate of plant cells.
Bernd Weisshaar - One of the best experts on this subject based on the ideXlab platform.
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The R2R3-MYB Gene family in banana (Musa acuminata): Genome-wide identification, classification and expression patterns.
PloS one, 2020Co-Authors: Boas Pucker, Ashutosh Pandey, Bernd Weisshaar, Ralf StrackeAbstract:The R2R3-MYB Genes comprise one of the largest transcription factor Gene families in plants, playing regulatory roles in plant-specific developmental processes, defense responses and metabolite accumulation. To date MYB family Genes have not yet been comprehensively identified in the major staple fruit crop banana. In this study, we present a comprehensive, genome-wide analysis of the MYB Genes from Musa acuminata DH-Pahang (A genome). A total of 285 R2R3-MYB Genes as well as Genes encoding three other classes of MYB proteins containing multiple MYB repeats were identified and characterised with respect to structure and chromosomal organisation. Organ- and development-specific expression patterns were determined from RNA-Seq data. For 280 M. acuminata MYB Genes for which expression was found in at least one of the analysed samples, a variety of expression patterns were detected. The M. acuminata R2R3-MYB Genes were functionally categorised, leading to the identification of seven clades containing only M. acuminata R2R3-MYBs. The encoded proteins may have specialised functions that were acquired or expanded in Musa during genome evolution. This functional classification and expression analysis of the MYB Gene family in banana establishes a solid foundation for future comprehensive functional analysis of MaMYBs and can be utilized in banana improvement programmes.
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The R2R3-MYB Gene family in banana (Musa acuminata): genome-wide identification, classification and expression patterns
2020Co-Authors: Boas Pucker, Ashutosh Pandey, Bernd Weisshaar, Ralf StrackeAbstract:The R2R3-MYB Genes comprise one of the largest transcription factor Gene families in plants, playing regulatory roles in plant-specific developmental processes, defense responses and metabolite accumulation. To date MYB family Genes have not yet been comprehensively identified in the major staple fruit crop banana. In this study, we present a comprehensive, genome-wide analysis of the MYB Genes from Musa acuminata DH-Pahang (A genome). A total of 286 R2R3-MYB Genes as well as Genes encoding three other classes of MYB proteins containing multiple MYB repeats were identified and characterised with respect to structure and chromosomal organisation. Organ- an delopment-specific expression patterns were determined from RNA-seq data. For 279 M. acuminata MYB Genes for which expression was found in at least one of the analysed samples, a variety of expression patterns were detected. The M. acuminata R2R3-MYB Genes were functionally categorised which led to the identification of seven clades containing only M. acuminata R2R3 MYBs. The encoded proteins may have specialised functions that were acquired or expanded in Musa during genome evolution. The presented functional classification and expression analyses of the MYB Gene family in banana establish a solid foundation for future comprehensive functional analysis of MaMYBs and can be utilized in banana improvement programmes.
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the r2r3 MYB Gene family in arabidopsis thaliana
Current Opinion in Plant Biology, 2001Co-Authors: Ralf Stracke, Martin Werber, Bernd WeisshaarAbstract:MYB factors represent a family of proteins that include the conserved MYB DNA-binding domain. In contrast to animals, plants contain a MYB-protein subfamily that is characterised by the R2R3-type MYB domain. ‘Classical’ MYB factors, which are related to c-MYB, seem to be involved in the control of the cell cycle in animals, plants and other higher eukaryotes. Systematic screens for knockout mutations in MYB Genes, followed by phenotypic analyses and the dissection of mutants with interesting phenotypes, have started to unravel the functions of the 125 R2R3-MYB Genes in Arabidopsis thaliana. R2R3-type MYB Genes control many aspects of plant secondary metabolism, as well as the identity and fate of plant cells.
Yixiong Tang - One of the best experts on this subject based on the ideXlab platform.
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the r2r3 MYB transcription factor Gene family in maize
PLOS ONE, 2012Co-Authors: Borun Feng, Sisi Yang, Yubi Huang, Yixiong TangAbstract:MYB proteins comprise a large family of plant transcription factors, members of which perform a variety of functions in plant biological processes. To date, no genome-wide characterization of this Gene family has been conducted in maize (Zea mays). In the present study, we performed a comprehensive computational analysis, to yield a complete overview of the R2R3-MYB Gene family in maize, including the phylogeny, expression patterns, and also its structural and functional characteristics. The MYB Gene structure in maize and Arabidopsis were highly conserved, indicating that they were originally compact in size. Subgroup-specific conserved motifs outside the MYB domain may reflect functional conservation. The genome distribution strongly supports the hypothesis that segmental and tandem duplication contribute to the expansion of maize MYB Genes. We also performed an updated and comprehensive classification of the R2R3-MYB Gene families in maize and other plant species. The result revealed that the functions were conserved between maize MYB Genes and their putative orthologs, demonstrating the origin and evolutionary diversification of plant MYB Genes. Species-specific groups/subgroups may evolve or be lost during evolution, resulting in functional divergence. Expression profile study indicated that maize R2R3-MYB Genes exhibit a variety of expression patterns, suggesting diverse functions. Furthermore, computational prediction potential targets of maize microRNAs (miRNAs) revealed that miR159, miR319, and miR160 may be implicated in regulating maize R2R3-MYB Genes, suggesting roles of these miRNAs in post-transcriptional regulation and transcription networks. Our comparative analysis of R2R3-MYB Genes in maize confirm and extend the sequence and functional characteristics of this Gene family, and will facilitate future functional analysis of the MYB Gene family in maize.
