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Edward B Breitschwerdt - One of the best experts on this subject based on the ideXlab platform.
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Infection with Hemotropic Mycoplasma Species in Patients with or without Extensive Arthropod or Animal Contact
Journal of clinical microbiology, 2013Co-Authors: Ricardo G Maggi, S. M. Compton, Chelsea L Trull, Patricia E. Mascarelli, Bobak Robert Mozayeni, Edward B BreitschwerdtAbstract:PCR amplification targeting the 16S rRNA gene was used to test individuals with and without extensive arthropod and animal contact for the possibility of hemotropic Mycoplasma infection. The prevalence of hemotropic Mycoplasma infection (4.7%) was significantly greater in previously reported cohorts of veterinarians, veterinary technicians, spouses of veterinary professionals, and others with extensive arthropod exposure and/or frequent animal contact than in a previously reported cohort of patients examined by a rheumatologist because of chronic joint pain or evidence of small-vessel disease (0.7%). Based upon DNA sequence analysis, a Mycoplasma ovis-like species was the most prevalent organism detected; however, infection with “Candidatus Mycoplasma haematoparvum” and a potentially novel, but incompletely characterized, hemotropic Mycoplasma species was also documented. Historical exposure to animals and arthropod vectors that can harbor hemotropic Mycoplasma spp. should be considered during epidemiological investigations and in the evaluation of individual patients.
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human coinfection with bartonella henselae and two hemotropic Mycoplasma variants resembling Mycoplasma ovis
Journal of Clinical Microbiology, 2010Co-Authors: Jane E Sykes, Leann L Lindsay, Ricardo G Maggi, Edward B BreitschwerdtAbstract:Two variants of an organism resembling the ovine hemoplasma, Mycoplasma ovis, were detected by PCR in blood samples from a veterinarian in Texas. Coinfection with similar variants has been described in sheep. This represents the first report of human infection with this organism. The veterinarian was coinfected with Bartonella henselae.
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CASE REPORTS Human Coinfection with Bartonella henselae and Two Hemotropic Mycoplasma Variants Resembling Mycoplasma ovis
2010Co-Authors: Jane E Sykes, Leann L Lindsay, Ricardo G Maggi, Edward B BreitschwerdtAbstract:CASE REPORT A 49-year-old man with a 15-year history of intermittent numbness of the left leg and left hand subsequently developed an acute febrile illness (39.2°C) with nausea and malaise that abated over a 1-week period. In the subsequent months, progressively severe neurological symptoms developed, which included stumbling during jogging, muscle weakness, and fatigue, which were thought to be associated with a viral neuropathy. During the next year, running became impossible, the patient could not walk unaided, he developed leg myoclonus and numbness of the hands, and resting two to three times a day was required. Routine laboratory testing was unremarkable. Magnetic resonance imaging (MRI), performed 6 years before and 6 months after the onset of febrile illness, showed progressive multifocal paraventricular lesions of the white matter in the brain and spinal cord suggestive of a demyelinating disease. Tibial nerve conduction velocities were slightly slowed, consistent with a mild to moderate demyelinating peripheral neuropathy. Cerebrospinal fluid analysis, which had been performed before the onset of febrile illness, revealed the presence of oligoclonal bands in the absence of corresponding serum bands. Ultimately, on the basis of these findings, multiple sclerosis (MS) was diagnosed. Methylprednisolone sodium succinate (1 g) was administered intravenously once daily for 5 days in early 2005, prior to the diagnosis of MS, and again
Sándor Hornok - One of the best experts on this subject based on the ideXlab platform.
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Tick- and fly-borne bacteria in ungulates: the prevalence of Anaplasma phagocytophilum, haemoplasmas and rickettsiae in water buffalo and deer species in Central Europe, Hungary
BMC Veterinary Research, 2018Co-Authors: Sándor Hornok, José De La ,fuente, László Sugár, Isabel G. Fernández De Mera, Gábor Horváth, Tibor Kovács, Attila Micsutka, Enikő Gönczi, Barbara Flaisz, Nóra TakácsAbstract:Background Hunting constitutes an important industry in Europe. However, data on the prevalence of vector-borne bacteria in large game animal species are lacking from several countries. Blood or spleen samples (239 and 270, respectively) were taken from red, fallow and roe deer, as well as from water buffaloes, mouflons and wild boars in Hungary, followed by DNA extraction and molecular analyses for Anaplasma phagocytophilum , haemoplasmas and rickettsiae. Results Based on blood samples, the prevalence rate of A. phagocytophilum infection was significantly higher in red deer (97.9%) than in fallow deer (72.7%) and roe deer (60%), and in all these compared to mouflons (6.3%). In addition, 39.2% of the spleen samples from wild boars were PCR positive for A. phagocytophilum , but none of the buffalos. Based on blood samples, the prevalence rates of both Mycoplasma wenyonii (Mw) and ‘ Candidatus M. haemobos’ (CMh) infections were significantly higher in buffaloes (Mw: 91.2%; CMh: 73.3%) than in red deer (Mw: 64.6%; CMh: 45.8%), and in both of them compared to fallow deer (Mw: 30.3%; CMh: 9.1%) and roe deer (Mw: 20%; CMh: 1.5%). The prevalence of Mw and CMh infection significantly correlated with the body sizes of these hosts. Furthermore, Mw was significantly more prevalent than CMh in buffaloes, red and roe deer. Mycoplasma ovis was detected in mouflons, M. suis in wild boars, R. helvetica in one fallow deer and one mouflon, and an unidentified Rickettsia sp. in a fallow deer. Conclusions Forest-dwelling game animal species were found to be important carriers of A. phagocytophilum . In contrast, animals grazing grassland (i.e. buffaloes) were less likely to get infected with this Ixodes ricinus -borne pathogen. Water buffaloes, deer species, mouflons and wild boars harbored haemoplasmas that may affect domestic ungulates. Evaluated animals with larger body size had significantly higher prevalence of infection with haemoplasmas compared to smaller deer species. The above host species rarely carried rickettsiae.
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Tick- and fly-borne bacteria in ungulates: the prevalence of Anaplasma phagocytophilum, haemoplasmas and rickettsiae in water buffalo and deer species in Central Europe, Hungary.
BMC veterinary research, 2018Co-Authors: Sándor Hornok, José De La ,fuente, László Sugár, Gábor Horváth, Tibor Kovács, Attila Micsutka, Enikő Gönczi, Barbara Flaisz, Isabel G. Fernández De Mera, Nóra TakácsAbstract:Hunting constitutes an important industry in Europe. However, data on the prevalence of vector-borne bacteria in large game animal species are lacking from several countries. Blood or spleen samples (239 and 270, respectively) were taken from red, fallow and roe deer, as well as from water buffaloes, mouflons and wild boars in Hungary, followed by DNA extraction and molecular analyses for Anaplasma phagocytophilum, haemoplasmas and rickettsiae. Based on blood samples, the prevalence rate of A. phagocytophilum infection was significantly higher in red deer (97.9%) than in fallow deer (72.7%) and roe deer (60%), and in all these compared to mouflons (6.3%). In addition, 39.2% of the spleen samples from wild boars were PCR positive for A. phagocytophilum, but none of the buffalos. Based on blood samples, the prevalence rates of both Mycoplasma wenyonii (Mw) and ‘Candidatus M. haemobos’ (CMh) infections were significantly higher in buffaloes (Mw: 91.2%; CMh: 73.3%) than in red deer (Mw: 64.6%; CMh: 45.8%), and in both of them compared to fallow deer (Mw: 30.3%; CMh: 9.1%) and roe deer (Mw: 20%; CMh: 1.5%). The prevalence of Mw and CMh infection significantly correlated with the body sizes of these hosts. Furthermore, Mw was significantly more prevalent than CMh in buffaloes, red and roe deer. Mycoplasma ovis was detected in mouflons, M. suis in wild boars, R. helvetica in one fallow deer and one mouflon, and an unidentified Rickettsia sp. in a fallow deer. Forest-dwelling game animal species were found to be important carriers of A. phagocytophilum. In contrast, animals grazing grassland (i.e. buffaloes) were less likely to get infected with this Ixodes ricinus-borne pathogen. Water buffaloes, deer species, mouflons and wild boars harbored haemoplasmas that may affect domestic ungulates. Evaluated animals with larger body size had significantly higher prevalence of infection with haemoplasmas compared to smaller deer species. The above host species rarely carried rickettsiae.
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Molecular characterization of two different strains of haemotropic Mycoplasmas from a sheep flock with fatal haemolytic anaemia and concomitant Anaplasma ovis infection.
Veterinary microbiology, 2008Co-Authors: Sándor Hornok, Marina L Meli, András Erdos, István Hajtós, Hans Lutz, Regina Hofmann-lehmannAbstract:After the first outbreak of fatal Mycoplasma ovis infection (eperythrozoonosis) in a sheep flock in Hungary (1997), a second wave of the disease was noted in 2006, with different seasonal pattern and affected age group, as well as increased mortality (5.5%). The aim of the present study was to molecularly characterize the causative agent and to reveal underlying factors of the second wave of the disease. Remarkably, among the 33 sheep examined, 17 were infected with two strains of haemotropic Mycoplasmas. Cloning and sequencing isolates of the latter showed that one of the strains was 99.4-99.8% identical to M. ovis (AF338268), while the second was only 96.8-97.9% identical and contained a 17-bp deletion. Different isolates of both strains were demonstrated in the same animal. When analyzing possible risk factors for fatal disease outcome, we found that among sheep born prior to the 1997 outbreak significantly more animals survived the second outbreak than succumbed to disease. In addition, locally born sheep were less frequently diseased than sheep introduced into the flock from other places. This suggests an immunoprotective effect in some animals. Concurrent infection with Anaplasma ovis was detected in 24 of the 33 evaluated sheep. In conclusion, this is the first study to demonstrate the existence of and characterize two genetically distinct ovine haemotropic Mycoplasma strains in a sheep flock with fatal haemolytic anaemia.
João Fábio Soares - One of the best experts on this subject based on the ideXlab platform.
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First molecular detection of Mycoplasma ovis (Hemotropic Mycoplasmas) from sheep in Brazil
Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia , 2019Co-Authors: Ugo Araújo Souza, Karina Oberrather, Renata Fagundes-moreira, Bruno Albuquerque De Almeida, Stella De Faria Valle, Aline Girotto-soares, João Fábio SoaresAbstract:Mycoplasma ovis is an emerging zoonotic pathogen with a worldwide distribution and can cause mild to severe hemolytic anemia, icterus, and poor weight gain in animals. Although M. ovis has been described in small ruminants worldwide, data on M. ovis in sheep in Brazil is unknown. The objective of the present study was to present the first report of hemotropic Mycoplasma (HM) in sheep from Brazil. We evaluated factors associated with this infection, such age group, tick presence, and anemia. Blood samples were collected from 33 sheep from a farm in southern Brazil and screened for hemoplasmas using PCR. Out of 33 samples, 26 (78.8%) tested positive for M. ovis. The sequencing of positive samples showed 100% identity with multiple M. ovis 16S rDNA sequences. No association was observed between the presence of M. ovis and the FAMACHA© score (p = 0.620). Rhipicephalus (Boophilus) microplus (15/33, 45.4%) was the tick species found on the animals. No significant association between M. ovis infection and presence of ticks (p = 0.4134) and age group (p = 0.4221) was observed. This is the first report of M. ovis infection in sheep from Brazil and only the second report of this pathogen in sheep in Latin America.
D.h. Aguirre - One of the best experts on this subject based on the ideXlab platform.
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Brote de micoplasmosis clínica por Mycoplasma ovis en ovinos de Salta, Argentina: Diagnóstico clínico, microbiológico y molecular
Revista Argentina de microbiologia, 2009Co-Authors: D.h. Aguirre, Carolina S. Thompson, R. D. Neumann, A. O. Salatin, A. B. Gaido, S. Torioni De EchaideAbstract:Clinical mycoplasmosis outbreak due to Mycoplasma ovis in sheep from Salta, Argentina. Clinical, microbiological and molecular diagnosis. Mycoplasma ovis is an obligatory parasite of the erythrocytes from small ruminants (sheep, goat), wherein it causes chronic or acute anaemia. This agent shows worldwide distribution. However, its dispersion is still unknown in Argentina. This work describes an outbreak of mycoplasmosis occurred in January 2007 in a sheep flock from Rosario de la Frontera, Salta, Argentina. Adult sheep became ill with a mortality rate of 17.8%. All blood smears (n = 11) examined by Giemsa stain showed the presence of small basophile bodies characteristic of M. ovis infection, indicating a high prevalence of the infection in the flock. The molecular diagnosis (n = 9) confirmed the findings through the amplification of two fragments from the 16S rRNA gene. This is the third report of M. ovis in Argentina and the first one concomitant with clinical signs at flock level.
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Brote de micoplasmosis clínica por Mycoplasma ovis en ovinos de Salta, Argentina: Diagnóstico clínico, microbiológico y molecular Clinical mycoplasmosis outbreak due to Mycoplasma ovis in sheep from Salta, Argentina: Clinical, microbiological and mol
Elsevier España S.L.U., 2009Co-Authors: D.h. Aguirre, R. D. Neumann, A. O. Salatin, A. B. Gaido, C. Thompson, Torioni S. De EchaideAbstract:Mycoplasma ovis es un parásito obligado de los eritrocitos de los pequeños rumiantes (ovinos, caprinos), en los que produce anemia crónica o aguda. Su distribución es mundial, aunque se desconoce la difusión de esta bacteria en la Argentina. Este trabajo describe un brote de micoplasmosis en un rebaño ovino de la localidad salteña de Rosario de la Frontera, ocurrido en enero de 2007. Durante ese brote resultó afectada la categoría de ovinos adultos, con una mortalidad del 17,8%. El diagnóstico en extendidos de sangre (tinción de Giemsa) reveló pequeños cuerpos basófilos, característicos de la infección por M. ovis, en todas las muestras examinadas (n = 11), lo que indica una alta prevalencia de la infección en la majada. El diagnóstico molecular (n = 9) confirmó los hallazgos mediante la amplificación de dos fragmentos del gen 16S rRNA. Este representa el tercer registro del microorganismo en la Argentina y el primero con expresión clínica a escala poblacional (rebaño).Mycoplasma ovis is an obligatory parasite of the erythrocytes from small ruminants (sheep, goat), wherein it causes chronic or acute anaemia. This agent shows worldwide distribution. However, its dispersion is still unknown in Argentina. This work describes an outbreak of mycoplasmosis occurred in January 2007 in a sheep flock from Rosario de la Frontera, Salta, Argentina. Adult sheep became ill with a mortality rate of 17.8%. All blood smears (n = 11) examined by Giemsa stain showed the presence of small basophile bodies characteristic of M. ovis infection, indicating a high prevalence of the infection in the flock. The molecular diagnosis (n = 9) confirmed the findings through the amplification of two fragments from the 16S rRNA gene. This is the third report of M. ovis in Argentina and the first one concomitant with clinical signs at flock level
Ricardo G Maggi - One of the best experts on this subject based on the ideXlab platform.
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Infection with Hemotropic Mycoplasma Species in Patients with or without Extensive Arthropod or Animal Contact
Journal of clinical microbiology, 2013Co-Authors: Ricardo G Maggi, S. M. Compton, Chelsea L Trull, Patricia E. Mascarelli, Bobak Robert Mozayeni, Edward B BreitschwerdtAbstract:PCR amplification targeting the 16S rRNA gene was used to test individuals with and without extensive arthropod and animal contact for the possibility of hemotropic Mycoplasma infection. The prevalence of hemotropic Mycoplasma infection (4.7%) was significantly greater in previously reported cohorts of veterinarians, veterinary technicians, spouses of veterinary professionals, and others with extensive arthropod exposure and/or frequent animal contact than in a previously reported cohort of patients examined by a rheumatologist because of chronic joint pain or evidence of small-vessel disease (0.7%). Based upon DNA sequence analysis, a Mycoplasma ovis-like species was the most prevalent organism detected; however, infection with “Candidatus Mycoplasma haematoparvum” and a potentially novel, but incompletely characterized, hemotropic Mycoplasma species was also documented. Historical exposure to animals and arthropod vectors that can harbor hemotropic Mycoplasma spp. should be considered during epidemiological investigations and in the evaluation of individual patients.
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human coinfection with bartonella henselae and two hemotropic Mycoplasma variants resembling Mycoplasma ovis
Journal of Clinical Microbiology, 2010Co-Authors: Jane E Sykes, Leann L Lindsay, Ricardo G Maggi, Edward B BreitschwerdtAbstract:Two variants of an organism resembling the ovine hemoplasma, Mycoplasma ovis, were detected by PCR in blood samples from a veterinarian in Texas. Coinfection with similar variants has been described in sheep. This represents the first report of human infection with this organism. The veterinarian was coinfected with Bartonella henselae.
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CASE REPORTS Human Coinfection with Bartonella henselae and Two Hemotropic Mycoplasma Variants Resembling Mycoplasma ovis
2010Co-Authors: Jane E Sykes, Leann L Lindsay, Ricardo G Maggi, Edward B BreitschwerdtAbstract:CASE REPORT A 49-year-old man with a 15-year history of intermittent numbness of the left leg and left hand subsequently developed an acute febrile illness (39.2°C) with nausea and malaise that abated over a 1-week period. In the subsequent months, progressively severe neurological symptoms developed, which included stumbling during jogging, muscle weakness, and fatigue, which were thought to be associated with a viral neuropathy. During the next year, running became impossible, the patient could not walk unaided, he developed leg myoclonus and numbness of the hands, and resting two to three times a day was required. Routine laboratory testing was unremarkable. Magnetic resonance imaging (MRI), performed 6 years before and 6 months after the onset of febrile illness, showed progressive multifocal paraventricular lesions of the white matter in the brain and spinal cord suggestive of a demyelinating disease. Tibial nerve conduction velocities were slightly slowed, consistent with a mild to moderate demyelinating peripheral neuropathy. Cerebrospinal fluid analysis, which had been performed before the onset of febrile illness, revealed the presence of oligoclonal bands in the absence of corresponding serum bands. Ultimately, on the basis of these findings, multiple sclerosis (MS) was diagnosed. Methylprednisolone sodium succinate (1 g) was administered intravenously once daily for 5 days in early 2005, prior to the diagnosis of MS, and again
