The Experts below are selected from a list of 429 Experts worldwide ranked by ideXlab platform
Won Ho Park - One of the best experts on this subject based on the ideXlab platform.
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Formation of Ag nanoparticles in PVA solution and catalytic activity of their electrospun PVA nanofibers
Fibers and Polymers, 2015Co-Authors: Ja Young Cheon, Yun Ok Kang, Won Ho ParkAbstract:Ag nanoparticles (NPs) were prepared by chemical reduction based on green synthesis in an aqueous poly(vinyl alcohol) (PVA) solution with different temperatures and pHs. The PVA and maltose were used as stabilizing and reducing agents, respectively. Silver nitrate (AgNO_3) precursor for Ag NPs was also used by 1 wt% on the base of the weight of PVA. The formation of Ag NPs was examined by UV-Vis spectrophotometer, and their size was measured by transmission electron microscopy (TEM), and nanoparticle size analyzer (NPSA). The formation rate of Ag NPs in the PVA solution increased with increasing temperature and pH, whereas the size of Ag NPs stabilized with PVA increased with increasing temperature, or with decreasing pH. Subsequently, the PVA Nanofibrous Matrix containing Ag NPs was prepared, by electrospinning PVA solution with Ag NPs, and followed by heat treatment. The morphology and crystalline structure of PVA nanofibers with Ag NPs was observed with field emission scanning electron microscopy (FE-SEM), and X-ray diffractometer (XRD), respectively. From the degradation reaction of methylene blue (MB) using PVA nanofibers web and film, it was found that the catalytic activity of PVA matrices with Ag NPs was strongly dependent on the surface area of the PVA matrices.
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Cellular response to poly(vinyl alcohol) nanofibers coated with biocompatible proteins and polysaccharides
Applied Surface Science, 2012Co-Authors: Da Hyun Jang, Lim Jeong, Hyun Ki Kang, Yun Ok Kang, Won Ho ParkAbstract:Abstract A PVA Nanofibrous Matrix was prepared by electrospinning an aqueous 10 wt% PVA solution. The mean diameter of the PVA nanofibers electrospun from the aqueous PVA solution was 240 nm. The water resistance of the as-spun PVA Nanofibrous Matrix was improved by physically crosslinking the PVA nanofibers by heat treatment at 150 °C for 10 min. In addition, the heat-treated PVA Nanofibrous Matrix was coated with biocompatible polysaccharides (chitosan (CHI) or hyaluronic acid (HA)) and proteins (collagen (COL) or silk fibroin (SF)) to construct biomimetic Nanofibrous scaffolds. The coating of proteins or polysaccharides on the PVA Nanofibrous Matrix was confirmed by ATR-IR spectra, and the degree of coating was determined by elemental analysis based on nitrogen content. The coated PVA matrices exhibited less hydrophilicity, except for the HA coating, and better tensile properties than the pure PVA Nanofibrous Matrix. The increase in tensile properties was due to interfiber bonds formed by the coating. The effect of protein and polysaccharide coating on normal human keratinocytes (NHEKs) and fibroblasts (NHEFs) was examined by cytocompatibility assessment in vitro. Among the CHI-, COL-, HA- and SF-coated PVA matrices, the SF-coated PVA Nanofibrous Matrix was found to be the most promising scaffold for the attachment and spreading of NHEKs and NHEFs as compared to the pure PVA Matrix. This approach to controlling the surface properties of Nanofibrous structures with SF may be useful in the design and tailoring of novel matrices for skin regeneration.
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Epidermal cellular response to poly(vinyl alcohol) nanofibers containing silver nanoparticles.
Colloids and Surfaces B: Biointerfaces, 2010Co-Authors: Ja Young Chun, Lim Jeong, Sung Youn Jung, Hyun Ki Kang, Yun Ok Kang, Ju-eun Oh, Won Ho ParkAbstract:Abstract A heat-treated PVA Nanofibrous Matrix containing silver (Ag) was prepared by electrospinning an aqueous 10 wt% PVA solution and followed by heat treatment at 150 °C for 10 min. The average diameter of the as-spun and heat-treated PVA nanofibers was 330 nm. The heat-treated PVA Nanofibrous Matrix containing Ag was irradiated with UV light to transform the Ag ions in the Nanofibrous Matrix into Ag nanoparticles. The in vitro cytotoxicity of the Ag ions and/or nanoparticles on normal human epidermal keratinocytes (NHEK) and fibroblasts (NHEF) cultures was examined. The PVA Nanofibrous Matrix containing Ag showed slightly higher level of attachment and spreading in the early stage culture (1 h) than the PVA nanofibers without Ag (control). However, compared with the PVA nanofibers without Ag, the heat-treated and UV-irradiated PVA nanofibers, containing mainly Ag ions and nanoparticles, respectively, showed reduced cell attachment and spreading. This shows that both Ag ions and Ag nanoparticles are cytotoxic to NHEK and NHEF. There was no significant difference in cytotoxicity to NHEK and NHEF between Ag ions and Ag nanoparticles. NHEF appeared to be more sensitive to Ag ions or particles than NHEK. In addition, the residual nitrate ions (NO3−) in the PVA nanofibers had an adverse effect on the culture of both cells.
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Chitosan‐coated poly(vinyl alcohol) nanofibers for wound dressings
Journal of Biomedical Materials Research Part B, 2009Co-Authors: Yun Ok Kang, Won Ho Park, In-soo Yoon, Samuel M. HudsonAbstract:A PVA Nanofibrous Matrix was prepared by electrospinning an aqueous 10 wt % PVA solution. The mean diameter of the PVA nanofibers electrospun from the PVA aqueous solution was 240 nm. The water resistance of the as-spun PVA Nanofibrous Matrix was improved by physically crosslinking the PVA nanofibers by heat treatment at 150°C for 10 min, which were found to be the optimal heat treatment conditions determined from chemical and morphological considerations. In addition, the heat-treated PVA (H-PVA) Nanofibrous Matrix was coated with a chitosan solution to construct biomimetic Nanofibrous wound dressings. The chitosan-coated PVA (C-PVA) Nanofibrous Matrix showed less hydrophilic and better tensile properties than the H-PVA Nanofibrous Matrix. The effect of the chitosan coating on open wound healing in a mouse was examined. The C-PVA and H-PVA Nanofibrous matrices showed faster wound healing than the control. The histological examination and mechanical stability revealed the C-PVA Nanofibrous Matrix to be more effective as a wound-healing accelerator in the early stages of wound healing than the H-PVA Nanofibrous Matrix. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2010
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Collagen-based biomimetic Nanofibrous scaffolds: Preparation and characterization of collagen/silk fibroin bicomponent Nanofibrous structures
Biomacromolecules, 2008Co-Authors: In Sung Yeo, Lim Jeong, Jun Eun Oh, Won Ho Park, Seung Jin Lee, Taek Seung Lee, Byung Moo MinAbstract:Electrospinning of collagen (COL)/silk fibroin (SF) blend solutions in 1,1,1,3,3,3-hexafluoro-2-propanol was investigated for fabrication of a biocompatible and biomimetic nanostructured scaffold for tissue engineering. The morphology of the electrospun COL/SF blend nanofibers was observed by scanning electron microscopy. The average diameters of COL/SF blend fibers ranged from 320 to 360 nm, irrespective of SF content in the blends. Both COL and SF components in the as-spun COL/SF blend matrices were stabilized by glutaraldehyde and water vapor, respectively, under the saturated glutaraldehyde aqueous solution at 25 degrees C. The glutaraldehyde vapor chemically stabilized the COL component via cross-linking, whereas the water vapor physically stabilized the SF component via crystallization to the beta-sheet structure. These structural changes of after-treated COL/SF blend matrices were examined using ATR-IR and CP/MAS (13)C NMR spectroscopy. To assay the cytocompatibility and cellular behavior of the COL/SF blend Nanofibrous scaffolds, cell attachment and the spreading of normal human epidermal keratinocytes (NHEK) and fibroblasts (NHEF) seeded on the scaffolds were studied. In addition, both morphological changes and cellular responses of COL/SF blend Nanofibrous matrices were also compared with COL/SF hybrid Nanofibrous matrices. Generally similar levels of cell attachment and spreading of NHEF were shown in the COL/SF blend Nanofibrous Matrix compared with those of the pure COL and pure SF matrices; the cellular responses of NHEK were, however, markedly decreased in the COL/SF blend Nanofibrous Matrix as compared to the pure matrices. In contrast, cell attachment and spreading of NHEK on the COL/SF hybrid Nanofibrous Matrix were significantly higher than that of the COL/SF blend Nanofibrous Matrix. Our results indicate that a COL/SF hybrid Nanofibrous Matrix may be a better candidate than a COL/SF blend Nanofibrous Matrix for biomedical applications such as wound dressing and scaffolds for tissue engineering.
Byung Moo Min - One of the best experts on this subject based on the ideXlab platform.
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Collagen-based biomimetic Nanofibrous scaffolds: Preparation and characterization of collagen/silk fibroin bicomponent Nanofibrous structures
Biomacromolecules, 2008Co-Authors: In Sung Yeo, Lim Jeong, Jun Eun Oh, Won Ho Park, Seung Jin Lee, Taek Seung Lee, Byung Moo MinAbstract:Electrospinning of collagen (COL)/silk fibroin (SF) blend solutions in 1,1,1,3,3,3-hexafluoro-2-propanol was investigated for fabrication of a biocompatible and biomimetic nanostructured scaffold for tissue engineering. The morphology of the electrospun COL/SF blend nanofibers was observed by scanning electron microscopy. The average diameters of COL/SF blend fibers ranged from 320 to 360 nm, irrespective of SF content in the blends. Both COL and SF components in the as-spun COL/SF blend matrices were stabilized by glutaraldehyde and water vapor, respectively, under the saturated glutaraldehyde aqueous solution at 25 degrees C. The glutaraldehyde vapor chemically stabilized the COL component via cross-linking, whereas the water vapor physically stabilized the SF component via crystallization to the beta-sheet structure. These structural changes of after-treated COL/SF blend matrices were examined using ATR-IR and CP/MAS (13)C NMR spectroscopy. To assay the cytocompatibility and cellular behavior of the COL/SF blend Nanofibrous scaffolds, cell attachment and the spreading of normal human epidermal keratinocytes (NHEK) and fibroblasts (NHEF) seeded on the scaffolds were studied. In addition, both morphological changes and cellular responses of COL/SF blend Nanofibrous matrices were also compared with COL/SF hybrid Nanofibrous matrices. Generally similar levels of cell attachment and spreading of NHEF were shown in the COL/SF blend Nanofibrous Matrix compared with those of the pure COL and pure SF matrices; the cellular responses of NHEK were, however, markedly decreased in the COL/SF blend Nanofibrous Matrix as compared to the pure matrices. In contrast, cell attachment and spreading of NHEK on the COL/SF hybrid Nanofibrous Matrix were significantly higher than that of the COL/SF blend Nanofibrous Matrix. Our results indicate that a COL/SF hybrid Nanofibrous Matrix may be a better candidate than a COL/SF blend Nanofibrous Matrix for biomedical applications such as wound dressing and scaffolds for tissue engineering.
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Electrospinning of collagen nanofibers: Effects on the behavior of normal human keratinocytes and early-stage wound healing
Biomaterials, 2006Co-Authors: Kyong Su Rho, Lim Jeong, Byoung Moo Seo, Seong Doo Hong, Sangho Roh, Jae Jin Cho, Won Ho Park, Yoon Jeong Park, Gene Lee, Byung Moo MinAbstract:Electrospinning of type I collagen in 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) to fabricate a biomimetic Nanofibrous extracellular Matrix for tissue engineering was investigated. The average diameter of collagen nanofibers electrospun from 8% collagen solution in HFIP was 460 nm (range of 100-1200 nm). The as-spun collagen Nanofibrous Matrix was chemically cross-linked by glutaraldehyde vapor with a saturated aqueous solution and then treated with aqueous 0.1 m glycine to block unreacted aldehyde groups. With vapor phase cross-linking for 12 h, porosity of the collagen Matrix decreased from 89% to 71%. The collagen Nanofibrous Matrix showed good tensile strength, even in aqueous solution. Effects on cytocompatibility, cell behavior, cell and collagen nanofiber interactions, and open wound healing in rats were examined. Relatively low cell adhesion was observed on uncoated collagen nanofibers, whereas collagen Nanofibrous matrices treated with type I collagen or laminin were functionally active in responses in normal human keratinocytes. Collagen Nanofibrous matrices were very effective as wound-healing accelerators in early-stage wound healing. Our results indicate that cross-linked collagen nanofibers coated with ECM proteins, particularly type I collagen, may be a good candidate for biomedical applications, such as wound dressing and scaffolds for tissue engineering. © 2005 Elsevier Ltd. All rights reserved.
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Biomimetic Nanofibrous scaffolds: Preparation and characterization of chitin/silk fibroin blend nanofibers
International Journal of Biological Macromolecules, 2006Co-Authors: Ko Eun Park, Byung Moo Min, Sung Youn Jung, Seung Jin Lee, Won Ho ParkAbstract:Electrospinning of chitin/silk fibroin (SF) blend solutions in 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) was investigated to fabricate a biomimetic nanostructured scaffolds for tissue engineering. The morphology of the electrospun chitin/SF blend nanofibers was investigated with a field emission scanning electron microscope (FE-SEM). The average diameters of chitin/SF blend fibers decreased from 920 to 340 nm, with the increase of chitin content in blend compositions. The miscibility of chitin/SF blend fibers was examined by solution viscosity measurement. The chitin and SF were immiscible in the as-spun Nanofibrous structure. The dimensional stability of chitin/SF blend nanofibers, with or without water vapor after-treatment, was conducted by immersing in water. As-spun SF-rich blend Nanofibrous matrices were lost their fibrous structure after the water immersion for 24 h, and then changed into membrane-like structure. On the contrary, Nanofibrous structures of water vapor-treated SF-rich blends were almost maintained. To assay the cytocompatibility and cell behavior on the chitin/SF blend Nanofibrous scaffolds, cell attachment and spreading of normal human epidermal keratinocyte and fibroblasts seeded on the scaffolds were studied. Our results indicate that chitin/SF blend Nanofibrous Matrix, particularly the one that contained 75% chitin and 25% SF, could be a potential candidate for tissue engineering scaffolds because it has both biomimetic three-dimensional structure and an excellent cell attachment and spreading for NHEK and NHEF. © 2006 Elsevier B.V. All rights reserved.
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Biomimetic Nanofibrous scaffolds: Preparation and characterization of PGA/chitin blend nanofibers
Biomacromolecules, 2006Co-Authors: Ko Eun Park, Byung Moo Min, Hyun Ki Kang, Seung Jin Lee, Won Ho ParkAbstract:Electrospinning of poly(glycolic acid) (PGA)/chitin blend solutions in 1,1,1,3,3,3-hexafluoro-2-propanol was investigated to fabricate biodegradable and biomimetic nanostructured scaffolds for tissue engineering. The morphology of the electrospun PGA/chitin blend nanofibers was investigated with a field emission scanning electron microscope. The PGA/chitin blend fibers have average diameters of around 140 nm, and their diameters have a distribution in the range 50-350 nm. The miscibility of PGA/chitin blend fibers was examined by differential scanning calorimetry. The PGA and chitin were immiscible in the as-spun Nanofibrous structure. An in vitro degradation study of PGA/chitin blend nanofibers was conducted in phosphate-buffered saline, pH 7.2. It was found that the hydrolytic cleavage of PGA in the blend nanofibers was accelerated by the coexistence of hydrophilie chitin. To assay the cytocompatibility and cell behavior on the PGA/chitin blend Nanofibrous scaffolds, cell attachment and spreading of normal human epidermal fibroblasts seeded on the scaffolds were studied. Our results indicate that the PGA/chitin blend Nanofibrous Matrix, particularly the one that contained 25% PGA and 75% chitin with bovine serum albumin coating, could be a good candidate for tissue engineering scaffolds, because it has an excellent cell attachment and spreading for normal human fibroblasts.
Seeram Ramakrishna - One of the best experts on this subject based on the ideXlab platform.
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Fabrication of a biomimetic ZeinPDA Nanofibrous scaffold impregnated with BMP-2 peptide conjugated TiO2 nanoparticle for bone tissue engineering.
Journal of Tissue Engineering and Regenerative Medicine, 2017Co-Authors: S. Babitha, Jayarama Reddy Venugopal, Seeram Ramakrishna, Meenakshi Annamalai, Michal Marcin Dykas, Surajit Saha, Kingshuk Poddar, Thirumalai Venkatesan, Purna Sai KorrapatiAbstract:A biomimetic Zein polydopamine (PDA) based nanofiber scaffold was fabricated to deliver bone morphogenic protein-2 (BMP-2) peptide conjugated titanium dioxide (TiO2) nanoparticles in a sustained manner for investigating its osteogenic differentiation potential. To prolong its retention time at the target site, BMP-2 peptide has been conjugated to TiO2 nanoparticles owing to its high surface to volume ratio. The effect of biochemical cues from BMP-2 peptide and nano topographical stimulation of electrospun Zein PDA nanofibers were examined for its enhanced osteogenic expression of human fetal osteoblast (hFOB) cells. The sustained delivery of bioactive signals, improved cell adhesion, mineralization and differentiation could be attributed to its highly interconnected Nanofibrous Matrix with unique material composition. Further, the expression of osteogenic markers revealed that the fabricated Nanofibrous scaffold possess better cell - biomaterial interactions. These promising results demonstrate the potential of the composite Nanofibrous scaffold as an effective biomaterial substrate for bone regeneration.
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Electrospun polyimide/titanium dioxide composite Nanofibrous membrane by electrospinning and electrospraying.
Journal of Nanoscience and Nanotechnology, 2011Co-Authors: Lijo F, Seeram Ramakrishna, Enrico Marsano, Chellappan Vijila, R.s. Barhate, Vijay Vk, ThavasiAbstract:Fibrous membrane with a fibre diameter of 229 +/- 35 nm was fabricated from polyimide solution by electrospinning. Nanofibrous membrane with a fibre diameter of 251 +/- 37 nm was fabricated by combined electrospinning and electrospraying for polyimide/TiO2. Among the different solvents studied, ethanol was the effective solvent for dispersing the TiO2 nanoparticles in the Nanofibrous Matrix during electrospraying. The average pore size of polyimide membrane was obtained in the range 0.79-0.89 microm whereas the average pore size of polyimide/TiO2 membrane was found to be in the range 1.23 microm. The tensile stress of polyimide Nanofibrous membrane and also polyimide/TiO2 composite fibrous membrane determined to be 0.36 MPa and 0.65 MPa respectively. Nanofibrous membrane containing TiO2 nanoparticles on the surface of the polyimide nanofibres improved the mechanical stability of the membrane.
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Electrospun Biocomposite Nanofibrous Scaffolds for Neural Tissue Engineering
Tissue Engineering Part A, 2008Co-Authors: Molamma P. Prabhakaran, Tan Ter Chyan, Lim Beng Hai, Aymeric Yutang Lim, Casey K. Chan, Jayarama Reddy Venugopal, Seeram RamakrishnaAbstract:Bridging of nerve gaps after injury is a major problem in peripheral nerve regeneration. Considering the potential application of a bio-artificial nerve guide material, polycaprolactone (PCL)/chitosan Nanofibrous scaffolds was designed and evaluated in vitro using rat Schwann cells (RT4-D6P2T) for nerve tissue engineering. PCL, chitosan, and PCL/chitosan nanofibers with average fiber diameters of 630, 450, and 190 nm, respectively, were fabricated using an electrospinning process. The surface chemistry of the fabricated nanofibers was determined using Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. Simple blending of PCL with chitosan proved an easy and efficient method for fabricating PCL/chitosan Nanofibrous scaffolds, whose surface characteristics proved more hydrophilic than PCL nanofibers. Evaluation of mechanical properties showed that the Young's modulus and strain at break of the electrospun PCL/chitosan nanofibers were better than those of the chitosan nanofibers. Results of cell proliferation studies on Nanofibrous scaffolds using 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium assay showed 48% more cell proliferation on PCL/chitosan scaffolds than on PCL scaffolds after 8 days of culture. PCL/chitosan scaffolds showed better cell proliferation than PCL scaffolds and maintained their characteristic cell morphology, with spreading bipolar elongations to the Nanofibrous substrates. This electrospun Nanofibrous Matrix thus proved of specific interest in tissue engineering for peripheral nerve regeneration.
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Mineralization of osteoblasts with electrospun collagen/hydroxyapatite nanofibers
Journal of Materials Science: Materials in Medicine, 2008Co-Authors: Jayarama Reddy Venugopal, Sharon Low, Aw Tar Choon, T. S. Sampath Kumar, Seeram RamakrishnaAbstract:Regeneration of fractured or diseased bones is the challenge faced by current technologies in tissue engineering. The major solid components of human bone consist of collagen and hydroxyapatite. Collagen (Col) and hydroxyapatite (HA) have potential in mimicking natural extracellular Matrix and replacing diseased skeletal bones. More attention has been focused on HA because of its crystallographic structure similar to inorganic compound found in natural bone and extensively investigated due to its excellent biocompatibility, bioactivity and osteoconductivity properties. In the present study, electrospun Nanofibrous scaffolds are fabricated with collagen (80 mg/ml) and Col/HA (1:1). The diameter of the collagen nanofibers is around 265 +/- 0.64 nm and Col/HA nanofibers are 293 +/- 1.45 nm. The crystalline HA (29 +/- 7.5 nm) loaded into the collagen nanofibers are embedded within Nanofibrous Matrix of the scaffolds. Osteoblasts cultured on both scaffolds and show insignificant level of proliferation but mineralization was significantly (p < 0.001) increased to 56% in Col/HA Nanofibrous scaffolds compared to collagen. Energy dispersive X-ray analysis (EDX) spectroscopy results proved the presence of higher level of calcium and phosphorous in Col/HA nanocomposites than collagen Nanofibrous scaffolds grown osteoblasts. The results of the present study suggested that the designed electrospun Nanofibrous scaffold (Col/HA) have potential biomaterial for bone tissue engineering.
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In vitro study of smooth muscle cells on polycaprolactone and collagen Nanofibrous matrices
Cell Biology International, 2005Co-Authors: Jayarama Reddy Venugopal, L.l. Ma, Thomas Yong, Seeram RamakrishnaAbstract:Biodegradable polycaprolactone and collagen nanofibers were produced by electrospinning, with fiber diameters of around 300–700 nm and features similar to the extracellular Matrix of natural tissue. Human coronary artery smooth muscle cells (SMCs) seeded on Nanofibrous matrices tend to maintain normal phenotypic shape and growth tends to be guided by the nanofiber orientation. The SMC and Nanofibrous Matrix interaction was observed by SEM, MTS assay, trypan blue exclusion method and laser scanning confocal microscopy. The results showed that the proliferation and growth rate of SMCs were not different on polycaprolactone (PCL) Nanofibrous matrices coated with collagen or tissue culture plates. PCL Nanofibrous matrices coated with collagen showed that the SMCs migrated towards inside the Nanofibrous matrices and formed smooth muscle tissue. This approach may be useful for engineering a variety of tissues in various structures and shapes, and also to demonstrate the importance of matching both the initial mechanical properties and degradation rate of Nanofibrous matrices to the specific tissue engineering.
S. C. Kundu - One of the best experts on this subject based on the ideXlab platform.
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Potential of inherent RGD containing silk fibroin–poly (Є-caprolactone) Nanofibrous Matrix for bone tissue engineering
Cell and Tissue Research, 2016Co-Authors: Promita Bhattacharjee, Banani Kundu, Deboki Naskar, T. K. Maiti, Debasis Bhattacharya, Hae Won Kim, S. C. KunduAbstract:The current study deals with the fabrication and characterization of blended Nanofibrous scaffolds of tropical tasar silk fibroin of Antheraea mylitta and poly (Є-caprolactone) to act as an ideal scaffold for bone regeneration. The use of poly (Є-caprolactone) in osteogenesis is well-recognized. At the same time, the osteoconductive nature of the non-mulberry tasar fibroin is also established due to its internal integrin binding peptide RGD (Arg-Gly-Asp) sequences, which enhance cellular interaction and proliferation. Considering that the materials have the required and favorable properties, the blends are formed using an equal volume ratio of fibroin (2 and 4 wt%) and poly (Є-caprolactone) solution (10 wt%) to fabricate nanofibers. The nanofibers possess an average diameter of 152 ± 18 nm (2 % fibroin/PCL) and 175 ± 15 nm (4 % fibroin/PCL). The results of Fourier transform infrared spectroscopy substantiates the preservation of the secondary structure of the fibroin in the blends indicating the structural stability of the neo-Matrix. With an increase in the fibroin percentage, the hydrophobicity and thermal stability of the matrices as measured from melting temperature T_m (using DSC) decrease, while the mechanical strength is improved. The blended Nanofibrous scaffolds are biodegradable, and support the viability and proliferation of human osteoblast-like cells as observed through scanning electron and confocal microscopes. Alkaline phosphatase assay indicates the cell proliferation and the generation of the neo-bone Matrix. Taken together, these findings illustrate that the silk–poly (Є-caprolactone) blended Nanofibrous scaffolds have an excellent prospect as scaffolding material in bone tissue engineering.
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potential of inherent rgd containing silk fibroin poly є caprolactone Nanofibrous Matrix for bone tissue engineering
Cell and Tissue Research, 2016Co-Authors: Promita Bhattacharjee, Banani Kundu, Deboki Naskar, T. K. Maiti, Debasis Bhattacharya, S. C. KunduAbstract:The current study deals with the fabrication and characterization of blended Nanofibrous scaffolds of tropical tasar silk fibroin of Antheraea mylitta and poly (Є-caprolactone) to act as an ideal scaffold for bone regeneration. The use of poly (Є-caprolactone) in osteogenesis is well-recognized. At the same time, the osteoconductive nature of the non-mulberry tasar fibroin is also established due to its internal integrin binding peptide RGD (Arg-Gly-Asp) sequences, which enhance cellular interaction and proliferation. Considering that the materials have the required and favorable properties, the blends are formed using an equal volume ratio of fibroin (2 and 4 wt%) and poly (Є-caprolactone) solution (10 wt%) to fabricate nanofibers. The nanofibers possess an average diameter of 152 ± 18 nm (2 % fibroin/PCL) and 175 ± 15 nm (4 % fibroin/PCL). The results of Fourier transform infrared spectroscopy substantiates the preservation of the secondary structure of the fibroin in the blends indicating the structural stability of the neo-Matrix. With an increase in the fibroin percentage, the hydrophobicity and thermal stability of the matrices as measured from melting temperature Tm (using DSC) decrease, while the mechanical strength is improved. The blended Nanofibrous scaffolds are biodegradable, and support the viability and proliferation of human osteoblast-like cells as observed through scanning electron and confocal microscopes. Alkaline phosphatase assay indicates the cell proliferation and the generation of the neo-bone Matrix. Taken together, these findings illustrate that the silk–poly (Є-caprolactone) blended Nanofibrous scaffolds have an excellent prospect as scaffolding material in bone tissue engineering.
Lim Jeong - One of the best experts on this subject based on the ideXlab platform.
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Cellular response to poly(vinyl alcohol) nanofibers coated with biocompatible proteins and polysaccharides
Applied Surface Science, 2012Co-Authors: Da Hyun Jang, Lim Jeong, Hyun Ki Kang, Yun Ok Kang, Won Ho ParkAbstract:Abstract A PVA Nanofibrous Matrix was prepared by electrospinning an aqueous 10 wt% PVA solution. The mean diameter of the PVA nanofibers electrospun from the aqueous PVA solution was 240 nm. The water resistance of the as-spun PVA Nanofibrous Matrix was improved by physically crosslinking the PVA nanofibers by heat treatment at 150 °C for 10 min. In addition, the heat-treated PVA Nanofibrous Matrix was coated with biocompatible polysaccharides (chitosan (CHI) or hyaluronic acid (HA)) and proteins (collagen (COL) or silk fibroin (SF)) to construct biomimetic Nanofibrous scaffolds. The coating of proteins or polysaccharides on the PVA Nanofibrous Matrix was confirmed by ATR-IR spectra, and the degree of coating was determined by elemental analysis based on nitrogen content. The coated PVA matrices exhibited less hydrophilicity, except for the HA coating, and better tensile properties than the pure PVA Nanofibrous Matrix. The increase in tensile properties was due to interfiber bonds formed by the coating. The effect of protein and polysaccharide coating on normal human keratinocytes (NHEKs) and fibroblasts (NHEFs) was examined by cytocompatibility assessment in vitro. Among the CHI-, COL-, HA- and SF-coated PVA matrices, the SF-coated PVA Nanofibrous Matrix was found to be the most promising scaffold for the attachment and spreading of NHEKs and NHEFs as compared to the pure PVA Matrix. This approach to controlling the surface properties of Nanofibrous structures with SF may be useful in the design and tailoring of novel matrices for skin regeneration.
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Epidermal cellular response to poly(vinyl alcohol) nanofibers containing silver nanoparticles.
Colloids and Surfaces B: Biointerfaces, 2010Co-Authors: Ja Young Chun, Lim Jeong, Sung Youn Jung, Hyun Ki Kang, Yun Ok Kang, Ju-eun Oh, Won Ho ParkAbstract:Abstract A heat-treated PVA Nanofibrous Matrix containing silver (Ag) was prepared by electrospinning an aqueous 10 wt% PVA solution and followed by heat treatment at 150 °C for 10 min. The average diameter of the as-spun and heat-treated PVA nanofibers was 330 nm. The heat-treated PVA Nanofibrous Matrix containing Ag was irradiated with UV light to transform the Ag ions in the Nanofibrous Matrix into Ag nanoparticles. The in vitro cytotoxicity of the Ag ions and/or nanoparticles on normal human epidermal keratinocytes (NHEK) and fibroblasts (NHEF) cultures was examined. The PVA Nanofibrous Matrix containing Ag showed slightly higher level of attachment and spreading in the early stage culture (1 h) than the PVA nanofibers without Ag (control). However, compared with the PVA nanofibers without Ag, the heat-treated and UV-irradiated PVA nanofibers, containing mainly Ag ions and nanoparticles, respectively, showed reduced cell attachment and spreading. This shows that both Ag ions and Ag nanoparticles are cytotoxic to NHEK and NHEF. There was no significant difference in cytotoxicity to NHEK and NHEF between Ag ions and Ag nanoparticles. NHEF appeared to be more sensitive to Ag ions or particles than NHEK. In addition, the residual nitrate ions (NO3−) in the PVA nanofibers had an adverse effect on the culture of both cells.
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Collagen-based biomimetic Nanofibrous scaffolds: Preparation and characterization of collagen/silk fibroin bicomponent Nanofibrous structures
Biomacromolecules, 2008Co-Authors: In Sung Yeo, Lim Jeong, Jun Eun Oh, Won Ho Park, Seung Jin Lee, Taek Seung Lee, Byung Moo MinAbstract:Electrospinning of collagen (COL)/silk fibroin (SF) blend solutions in 1,1,1,3,3,3-hexafluoro-2-propanol was investigated for fabrication of a biocompatible and biomimetic nanostructured scaffold for tissue engineering. The morphology of the electrospun COL/SF blend nanofibers was observed by scanning electron microscopy. The average diameters of COL/SF blend fibers ranged from 320 to 360 nm, irrespective of SF content in the blends. Both COL and SF components in the as-spun COL/SF blend matrices were stabilized by glutaraldehyde and water vapor, respectively, under the saturated glutaraldehyde aqueous solution at 25 degrees C. The glutaraldehyde vapor chemically stabilized the COL component via cross-linking, whereas the water vapor physically stabilized the SF component via crystallization to the beta-sheet structure. These structural changes of after-treated COL/SF blend matrices were examined using ATR-IR and CP/MAS (13)C NMR spectroscopy. To assay the cytocompatibility and cellular behavior of the COL/SF blend Nanofibrous scaffolds, cell attachment and the spreading of normal human epidermal keratinocytes (NHEK) and fibroblasts (NHEF) seeded on the scaffolds were studied. In addition, both morphological changes and cellular responses of COL/SF blend Nanofibrous matrices were also compared with COL/SF hybrid Nanofibrous matrices. Generally similar levels of cell attachment and spreading of NHEF were shown in the COL/SF blend Nanofibrous Matrix compared with those of the pure COL and pure SF matrices; the cellular responses of NHEK were, however, markedly decreased in the COL/SF blend Nanofibrous Matrix as compared to the pure matrices. In contrast, cell attachment and spreading of NHEK on the COL/SF hybrid Nanofibrous Matrix were significantly higher than that of the COL/SF blend Nanofibrous Matrix. Our results indicate that a COL/SF hybrid Nanofibrous Matrix may be a better candidate than a COL/SF blend Nanofibrous Matrix for biomedical applications such as wound dressing and scaffolds for tissue engineering.
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Electrospinning of collagen nanofibers: Effects on the behavior of normal human keratinocytes and early-stage wound healing
Biomaterials, 2006Co-Authors: Kyong Su Rho, Lim Jeong, Byoung Moo Seo, Seong Doo Hong, Sangho Roh, Jae Jin Cho, Won Ho Park, Yoon Jeong Park, Gene Lee, Byung Moo MinAbstract:Electrospinning of type I collagen in 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) to fabricate a biomimetic Nanofibrous extracellular Matrix for tissue engineering was investigated. The average diameter of collagen nanofibers electrospun from 8% collagen solution in HFIP was 460 nm (range of 100-1200 nm). The as-spun collagen Nanofibrous Matrix was chemically cross-linked by glutaraldehyde vapor with a saturated aqueous solution and then treated with aqueous 0.1 m glycine to block unreacted aldehyde groups. With vapor phase cross-linking for 12 h, porosity of the collagen Matrix decreased from 89% to 71%. The collagen Nanofibrous Matrix showed good tensile strength, even in aqueous solution. Effects on cytocompatibility, cell behavior, cell and collagen nanofiber interactions, and open wound healing in rats were examined. Relatively low cell adhesion was observed on uncoated collagen nanofibers, whereas collagen Nanofibrous matrices treated with type I collagen or laminin were functionally active in responses in normal human keratinocytes. Collagen Nanofibrous matrices were very effective as wound-healing accelerators in early-stage wound healing. Our results indicate that cross-linked collagen nanofibers coated with ECM proteins, particularly type I collagen, may be a good candidate for biomedical applications, such as wound dressing and scaffolds for tissue engineering. © 2005 Elsevier Ltd. All rights reserved.
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Electrospinning of collagen nanofibers: effects on the behavior of normal human keratinocytes and early-stage wound healing.
Biomaterials, 2005Co-Authors: Lim Jeong, Seong Doo Hong, Yoon Jeong Park, Won Ho ParkAbstract:Abstract Electrospinning of type I collagen in 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) to fabricate a biomimetic Nanofibrous extracellular Matrix for tissue engineering was investigated. The average diameter of collagen nanofibers electrospun from 8% collagen solution in HFIP was 460 nm (range of 100–1200 nm). The as-spun collagen Nanofibrous Matrix was chemically cross-linked by glutaraldehyde vapor with a saturated aqueous solution and then treated with aqueous 0.1 m glycine to block unreacted aldehyde groups. With vapor phase cross-linking for 12 h, porosity of the collagen Matrix decreased from 89% to 71%. The collagen Nanofibrous Matrix showed good tensile strength, even in aqueous solution. Effects on cytocompatibility, cell behavior, cell and collagen nanofiber interactions, and open wound healing in rats were examined. Relatively low cell adhesion was observed on uncoated collagen nanofibers, whereas collagen Nanofibrous matrices treated with type I collagen or laminin were functionally active in responses in normal human keratinocytes. Collagen Nanofibrous matrices were very effective as wound-healing accelerators in early-stage wound healing. Our results indicate that cross-linked collagen nanofibers coated with ECM proteins, particularly type I collagen, may be a good candidate for biomedical applications, such as wound dressing and scaffolds for tissue engineering.
