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Felipe Ascencio - One of the best experts on this subject based on the ideXlab platform.
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apoptosis of hemocytes from lions paw scallop Nodipecten subnodosus induced with paralyzing shellfish poison from gymnodinium catenatum
Immunobiology, 2014Co-Authors: Norma Estrada, Felipe Ascencio, L Shoshani, R G ContrerasAbstract:Abstract The toxic dinoflagellate Gymnodinium catenatum produces paralyzing shellfish poisons (PSPs) that are consumed and accumulated by bivalves. Previously, we recorded a decrease in hemocytes 24 h after injection of PSPs (gonyautoxin 2/3 epimers, GTX2/3) in the adductor muscle in the lions-paw scallop Nodipecten subnodosus . In this work, qualitative and quantitative analyses, in in vivo and in vitro experiments, revealed that the lower count of hemocytes results from cells undergoing typical apoptosis when exposed to GTX 2/3 epimers. This includes visible morphological alterations of the cytoplasmic membrane, damage to the nuclear membrane, condensation of chromatin, DNA fragmentation, and release of DNA fragments into the cytoplasm. Induction of apoptosis was accompanied by phosphatidylserine exposure to the outer cell membrane and activation of cysteine-aspartic proteases, caspase 3 and caspase 8. Addition of an inhibitor of caspase to the medium suppressed activation in hemocytes exposed to the toxins, suggesting that cell death was induced by a caspase-dependent apoptotic pathway. The results are important for future investigation of the scallop's immune system and should provide new insights into apoptotic processes in immune cells of scallops exposed to PSPs.
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morphofunctional study of hemocytes from lions paw scallop Nodipecten subnodosus
Immunobiology, 2013Co-Authors: Norma Estrada, Edwin Velazquez, Carmen Rodriguezjaramillo, Felipe AscencioAbstract:Abstract Hemocytes play an important role in internal defense in mollusk bivalves; they are generally divided into granular and hyaline types, where the granules possess molecules to combat foreign particles. We investigated the morphology, staining reactions, and immune-related activities of hemocytes from the pectinid lions-paw scallop Nodipecten subnodosus . We showed that, in contrast to the conditions in most bivalves and similar to other pectinids, no granular cells were observed; only very few semi-granular cells were present and the large majority of cells were hyalinocytes and blast-like cells. The cytoplasm in many hyalinocytes showed vesicles of various sizes, but none with the staining characteristics of typical granules. When hemolymph was removed from these scallops, the plasma did not clot and the hemocytes rapidly adhered to one another in suspension. When living hemocytes were placed on a glass surface, they clustered into groups of various sizes; within minutes, pseudopods extended from the cells and outward migration began. Many cells spread to form extensive networks of flat cells, and other undifferentiated cells did not spread and kept a rounded morphology. Some hemocytes were immunologically active because they phagocytize Escherichia coli bacteria. Hemocytes expressed the carbohydrates residues N-acetyl- d -glucosamine, α- d -glucose, α- d -mannose, and α-L-fucose moieties, and cytochemical assays showed activity of acid phosphatase, specific esterase, and non-specific esterase, enzymes commonly associated with lysosomes and immunologic activity. These observations serve as a useful tool for further investigation of morphofunctional studies and physiological status of scallops.
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Effects of induced paralysis on hemocytes and tissues of the giant lions-paw scallop by paralyzing shellfish poison
Marine Biology, 2010Co-Authors: Norma Estrada, Gerardo Contreras, Carmen Rodríguez-jaramillo, Felipe AscencioAbstract:In general, bivalves are not affected by exposure to toxic dinoflagellates that produce paralyzing shellfish poisons (PSP). After injection with PSP extracted from the Gymnodinium catenatum, Nodipecten subnodosus is paralyzed, indicating that PSP provokes effects similar to what is observed in vertebrates, including paralysis and metabolic stress. To investigate the processes involved in poisoning by PSP, lions-paw scallops were injected with gonyautoxin (GTX) 2/3 epimers in the adductor muscle. Mild doses provoked adductor muscle contractions and paralysis, mantle retraction, and incapacity of shell closure, but scallops gradually recovered in a clear, dose-time recovery pattern. With high doses of GTX 2/3, scallops were permanently paralyzed, and hemocytes in hemolymph were reduced. Surprisingly, under these conditions, scallops continued normal feeding and did not show any microscopic defect in intestine or gills, but hemocytes infiltrated the adductor muscle and abnormal vitellogenesis and mantle melanization occurred. Paralysis stress was accompanied by negative scallop responses, based on visible effects, generation of nitric oxide, lipid peroxidation, and changes in antioxidant and hydrolytic enzymes in hemocytes and tissues. These data can be used to understand potential side effects of PSP in bivalves.
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Efecto de bacterias probióticas en el cultivo larvario del ostión de placer Crassostrea corteziensis (Bivalvia: Ostreidae)
Revista De Biologia Tropical, 2010Co-Authors: Ángel I. Campa-córdova, Felipe Ascencio, Gabriel Aguirre-guzmán, Antonio Luna-gonzález, José Manuel Mazón-suástegui, Héctor A. González-ocampoAbstract:Effect of probiotic bacteria on survival and growth of Cortez oyster larvae, Crassostrea cor- teziensis (Bivalvia: Ostreidae). Disease control problems have major constraints in aquaculture production, and the use of probiotics in larviculture is a valid alternative to antibiotics. This study analyzed the effect of probi- otic bacteria on survival and final size of Cortez oyster larvae Crassostrea corteziensis. Two different probiotic concentrations were evaluated, 1x10 4 and 1x10 5 CFU/ml of Lactic acid bacteria (strain NS61) isolated from Nodipecten subnodosus, and bacilli isolated from the white leg shrimp, Litopenaeus vannamei (Pseudomonas aeruginosa, strain YC58) and C. corteziensis (Burkholderia cepacia, strain Y021). Bacteria were added directly into culture tanks, starting the bioassays from veliger to pediveliger stages as follows: (1) Control, without probiotics; (2) lactic acid bacteria (Lb); (3) bacilli mix (Mb) in a proportion 1:1. Results showed a higher larval survival with Lb and Mb at a dose of 1x10 4 CFU/ml compared to the control group. Larvae exposed to Mb at 1x10 5 CFU/ml showed higher survival than Lb and control. Larval final size was not significantly increased with the tested probiotics, but larvae treated with Lb at 1x10 5 CFU/ml showed less survival rate than those treated at 1x10 4 CFU/ml. This study showed the beneficial effect of these probiotics, added individually or mixed in C.
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Growth, survival, and superoxide dismutase activity in juvenile Crassostrea corteziensis (Hertlein, 1951) treated with probiotics
Hidrobiologica, 2009Co-Authors: Ángel I. Campa-córdova, Antonio Luna-gonzález, Héctor A. González-ocampo, José Manuel Mazón-suástegui, Felipe AscencioAbstract:Juvenile seed of the Cortes oyster Crassostrea corteziensis were exposed to Lactobacillus sp. isolated from Nodipecten subnodosus, a mix of Pseudomonas sp. and Burkholderia cepacia, a marine yeast strain, a commercial probiotic (Epicin®), and oxytetracycline to determine their effect on growth, survival, SOD activity, and protein content. Probiotics at the test dose of 50,000 cells •ml-1, Epicin and oxytetracycline at 7 mg •l -1 were evaluated during 30 days of culture. Results showed that growth of C. corteziensis was significantly improved by Lactobacillus sp. and the bacilli mix significantly enhanced survival and SOD activity at the test dose. Protein content did not significantly increase by the treatments used. This study demonstrated the potential use of marine microbiota to improve cultivation of C. corteziensis.
Norma Estrada - One of the best experts on this subject based on the ideXlab platform.
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apoptosis of hemocytes from lions paw scallop Nodipecten subnodosus induced with paralyzing shellfish poison from gymnodinium catenatum
Immunobiology, 2014Co-Authors: Norma Estrada, Felipe Ascencio, L Shoshani, R G ContrerasAbstract:Abstract The toxic dinoflagellate Gymnodinium catenatum produces paralyzing shellfish poisons (PSPs) that are consumed and accumulated by bivalves. Previously, we recorded a decrease in hemocytes 24 h after injection of PSPs (gonyautoxin 2/3 epimers, GTX2/3) in the adductor muscle in the lions-paw scallop Nodipecten subnodosus . In this work, qualitative and quantitative analyses, in in vivo and in vitro experiments, revealed that the lower count of hemocytes results from cells undergoing typical apoptosis when exposed to GTX 2/3 epimers. This includes visible morphological alterations of the cytoplasmic membrane, damage to the nuclear membrane, condensation of chromatin, DNA fragmentation, and release of DNA fragments into the cytoplasm. Induction of apoptosis was accompanied by phosphatidylserine exposure to the outer cell membrane and activation of cysteine-aspartic proteases, caspase 3 and caspase 8. Addition of an inhibitor of caspase to the medium suppressed activation in hemocytes exposed to the toxins, suggesting that cell death was induced by a caspase-dependent apoptotic pathway. The results are important for future investigation of the scallop's immune system and should provide new insights into apoptotic processes in immune cells of scallops exposed to PSPs.
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morphofunctional study of hemocytes from lions paw scallop Nodipecten subnodosus
Immunobiology, 2013Co-Authors: Norma Estrada, Edwin Velazquez, Carmen Rodriguezjaramillo, Felipe AscencioAbstract:Abstract Hemocytes play an important role in internal defense in mollusk bivalves; they are generally divided into granular and hyaline types, where the granules possess molecules to combat foreign particles. We investigated the morphology, staining reactions, and immune-related activities of hemocytes from the pectinid lions-paw scallop Nodipecten subnodosus . We showed that, in contrast to the conditions in most bivalves and similar to other pectinids, no granular cells were observed; only very few semi-granular cells were present and the large majority of cells were hyalinocytes and blast-like cells. The cytoplasm in many hyalinocytes showed vesicles of various sizes, but none with the staining characteristics of typical granules. When hemolymph was removed from these scallops, the plasma did not clot and the hemocytes rapidly adhered to one another in suspension. When living hemocytes were placed on a glass surface, they clustered into groups of various sizes; within minutes, pseudopods extended from the cells and outward migration began. Many cells spread to form extensive networks of flat cells, and other undifferentiated cells did not spread and kept a rounded morphology. Some hemocytes were immunologically active because they phagocytize Escherichia coli bacteria. Hemocytes expressed the carbohydrates residues N-acetyl- d -glucosamine, α- d -glucose, α- d -mannose, and α-L-fucose moieties, and cytochemical assays showed activity of acid phosphatase, specific esterase, and non-specific esterase, enzymes commonly associated with lysosomes and immunologic activity. These observations serve as a useful tool for further investigation of morphofunctional studies and physiological status of scallops.
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Effects of induced paralysis on hemocytes and tissues of the giant lions-paw scallop by paralyzing shellfish poison
Marine Biology, 2010Co-Authors: Norma Estrada, Gerardo Contreras, Carmen Rodríguez-jaramillo, Felipe AscencioAbstract:In general, bivalves are not affected by exposure to toxic dinoflagellates that produce paralyzing shellfish poisons (PSP). After injection with PSP extracted from the Gymnodinium catenatum, Nodipecten subnodosus is paralyzed, indicating that PSP provokes effects similar to what is observed in vertebrates, including paralysis and metabolic stress. To investigate the processes involved in poisoning by PSP, lions-paw scallops were injected with gonyautoxin (GTX) 2/3 epimers in the adductor muscle. Mild doses provoked adductor muscle contractions and paralysis, mantle retraction, and incapacity of shell closure, but scallops gradually recovered in a clear, dose-time recovery pattern. With high doses of GTX 2/3, scallops were permanently paralyzed, and hemocytes in hemolymph were reduced. Surprisingly, under these conditions, scallops continued normal feeding and did not show any microscopic defect in intestine or gills, but hemocytes infiltrated the adductor muscle and abnormal vitellogenesis and mantle melanization occurred. Paralysis stress was accompanied by negative scallop responses, based on visible effects, generation of nitric oxide, lipid peroxidation, and changes in antioxidant and hydrolytic enzymes in hemocytes and tissues. These data can be used to understand potential side effects of PSP in bivalves.
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effects of the toxic dinoflagellate gymnodinium catenatum on hydrolytic and antioxidant enzymes in tissues of the giant lions paw scallop Nodipecten subnodosus
Comparative Biochemistry and Physiology C-toxicology & Pharmacology, 2007Co-Authors: Norma Estrada, Angel I Campacordova, Maria De Jesus Romero, Antonio Luna, Felipe AscencioAbstract:Abstract This study documents effects of the toxic dinoflagellate Gymnodinium catenatum, a producer of paralytic shellfish poison, on juvenile farmed (5.9 ± 0.39 cm) giant lions-paw scallop Nodipecten subnodosus. Scallops were fed bloom concentrations of toxic dinoflagellate G. catenatum for 7 h. The effect of the toxic dinoflagellate in different tissues was determined by analysis of antioxidant enzymes (catalase, superoxide dismutase, gluthathione peroxidase), thiobarbituric acid reactive substances (lipid peroxidation), and hydrolytic enzymes (proteases, glycosidases, phosphatases, lipases, and esterases). Histopathological photos record the effects of the toxic dinoflagellate in various tissues. The results show that juvenile lions-paw scallops produce pseudo-feces, partially close their shell, increase melanization, and aggregate hemocytes. Several enzymes were affected and could serve as biological markers. In general, the adductor muscle was not affected. In the digestive gland, some enzymes could be the result of defensive and digestive processes. Gills and mantle tissue were markedly affected because these sites respond first to toxic dinoflagellates, leading to the idea that proteolytic cascades could be involved.
Angel I Campacordova - One of the best experts on this subject based on the ideXlab platform.
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isolation and use of beneficial microbiota from the digestive tract of lions paw scallop Nodipecten subnodosus and winged pearl oyster pteria sterna in oyster aquaculture
Aquaculture Research, 2016Co-Authors: Fernando Abasolopacheco, Ruben Araya, Dariel Tovarramirez, Jesus M Ramirezorozco, Jose Manuel Mazonsuastegui, Pedro E. Saucedo, Angel I CampacordovaAbstract:We isolated microbiota from the digestive tract of Nodipecten subnodosus and Pteria sterna and determined in vitro their haemolytic activity, antagonism against Vibrio spp., bacterial hydrophobicity, production of extracellular enzymes and molecular identification. Five bacterial strains were selected: RL5 and C3 (Lactobacillus spp.) and PB1-1, PB1-5 and PB1-6 (Bacillus spp.). The RL5 and C3 isolates showed antimicrobial activity against Vibrio spp. and the PB1-1, PB1-5 and PB1-6 isolates showed enzymatic activity for amylase, protease, lipase and cellulose; the C3 and PB1-5 isolates were highly hydrophobic. The selected strains of bacteria were tested in vivo as probiotics, together with a treatment of ampicillin and a control without bacteria on juvenile Kumamoto oysters Crassostrea sikamea. The strains were provided individually and as mixes of isolates. Survival, growth and biochemical composition of the juveniles were determined as in vivo indicators. Juveniles grew significantly larger and faster when treated with a specific mix of isolates (MIX-B), compared with the control. The protein, lipid and carbohydrate concentrations were also significantly higher in oysters exposed to probiotic treatments, compared with the control and the antibiotic treatment. The selected microbiota showed probiotic proprieties for cultivating C. sikamea juveniles.
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growth survival and enzyme activity of lions paw scallop Nodipecten subnodosus spat treated with probiotics at the hatchery
Aquaculture Research, 2012Co-Authors: Andres Granadosamores, Angel I Campacordova, Ruben Araya, Jose Manuel Mazonsuastegui, Pedro E. SaucedoAbstract:Different types and concentrations of probiotics were evaluated for their effects on growth, survival and enzyme activity of hatchery-reared Nodipecten subnodosus spat. The treatments included (1) a mix of the microalgae Isochrysis galbana and Pavlova salina as the control group; (2) Diet 1 + a mix of Burkholderia cepacia and Pseudomonas aeruginosa; (3) Diet 1 + the commercial probiotic Epicin; and (4) Diet 1 + the commercial probiotic Bactosafe. Both commercial probiotics were tested at 3, 6 and 10 mg L 1 . Scallops receiving Bactosafe and Epicin at 3 and 6 mg L 1 grew ~30% faster and larger than in the control group. Regardless of the treatment, survival at the end was 98.7%. There was no relation between the activity of catalase and superoxide dismutase; however, significantly lower catalase levels occurred at increasing doses of Epicin; significant higher catalase levels occurred in the control group and mix of bacilli. Compared with the control group, lysozyme levels were significantly higher with the Epicin and Bactosafe treatments (3 and 6 mg L 1 ). More research needs to be conducted with probiotics to determine their real effects on the activity of antioxidant enzymes. We expect that treated scallops will increase their performance at the field.
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superoxide dismutase activity in juvenile litopenaeus vannamei and Nodipecten subnodosus exposed to the toxic dinoflagellate prorocentrum lima
Comparative Biochemistry and Physiology C-toxicology & Pharmacology, 2009Co-Authors: Angel I Campacordova, Antonio Lunagonzalez, Erick J Nunezvazquez, Maria De Jesus Romerogeraldo, Felipe AscencioAbstract:Abstract The toxic effect of the dinoflagellate Prorocentrum lima on juvenile American whiteleg shrimp Litopenaeus vannamei and giant lion-paw scallop Nodipecten subnodosus was evaluated. Organisms were exposed to three densities (500, 2000, or 5000 cells mL− 1), superoxide dismutase activity and soluble protein in the hepatopancreas and muscle were determined at 1, 6, 24 and 48 h after challenge. Shrimp exposed at 5000 cells mL− 1 significantly increased SOD activity in the hepatopancreas at 1 h post-challenge, whereas enzymatic activity in muscle significantly increased at 24 h at all densities. Scallops exposed to 500 and 2000 cells mL− 1 showed significant SOD activity increase in hepatopancreas at 24 and 12 h, respectively. Mortality at 48 h was 100% in scallops exposed to 5000 cells mL− 1. Shrimp showed higher levels of SOD activity than scallops. Soluble protein content in the shrimp hepatopancreas was significantly higher at densities of 500 and 2000 cells mL− 1 at 6 and 1 h, respectively. Soluble protein content in the scallop hepatopancreas was higher than control values at 1 h after challenge. In this study, 500 cells mL− 1 was enough to trigger SOD activity in two benthic species exposed to the toxic dinoflagellate P. lima.
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effects of the toxic dinoflagellate gymnodinium catenatum on hydrolytic and antioxidant enzymes in tissues of the giant lions paw scallop Nodipecten subnodosus
Comparative Biochemistry and Physiology C-toxicology & Pharmacology, 2007Co-Authors: Norma Estrada, Angel I Campacordova, Maria De Jesus Romero, Antonio Luna, Felipe AscencioAbstract:Abstract This study documents effects of the toxic dinoflagellate Gymnodinium catenatum, a producer of paralytic shellfish poison, on juvenile farmed (5.9 ± 0.39 cm) giant lions-paw scallop Nodipecten subnodosus. Scallops were fed bloom concentrations of toxic dinoflagellate G. catenatum for 7 h. The effect of the toxic dinoflagellate in different tissues was determined by analysis of antioxidant enzymes (catalase, superoxide dismutase, gluthathione peroxidase), thiobarbituric acid reactive substances (lipid peroxidation), and hydrolytic enzymes (proteases, glycosidases, phosphatases, lipases, and esterases). Histopathological photos record the effects of the toxic dinoflagellate in various tissues. The results show that juvenile lions-paw scallops produce pseudo-feces, partially close their shell, increase melanization, and aggregate hemocytes. Several enzymes were affected and could serve as biological markers. In general, the adductor muscle was not affected. In the digestive gland, some enzymes could be the result of defensive and digestive processes. Gills and mantle tissue were markedly affected because these sites respond first to toxic dinoflagellates, leading to the idea that proteolytic cascades could be involved.
Pedro E. Saucedo - One of the best experts on this subject based on the ideXlab platform.
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isolation and use of beneficial microbiota from the digestive tract of lions paw scallop Nodipecten subnodosus and winged pearl oyster pteria sterna in oyster aquaculture
Aquaculture Research, 2016Co-Authors: Fernando Abasolopacheco, Ruben Araya, Dariel Tovarramirez, Jesus M Ramirezorozco, Jose Manuel Mazonsuastegui, Pedro E. Saucedo, Angel I CampacordovaAbstract:We isolated microbiota from the digestive tract of Nodipecten subnodosus and Pteria sterna and determined in vitro their haemolytic activity, antagonism against Vibrio spp., bacterial hydrophobicity, production of extracellular enzymes and molecular identification. Five bacterial strains were selected: RL5 and C3 (Lactobacillus spp.) and PB1-1, PB1-5 and PB1-6 (Bacillus spp.). The RL5 and C3 isolates showed antimicrobial activity against Vibrio spp. and the PB1-1, PB1-5 and PB1-6 isolates showed enzymatic activity for amylase, protease, lipase and cellulose; the C3 and PB1-5 isolates were highly hydrophobic. The selected strains of bacteria were tested in vivo as probiotics, together with a treatment of ampicillin and a control without bacteria on juvenile Kumamoto oysters Crassostrea sikamea. The strains were provided individually and as mixes of isolates. Survival, growth and biochemical composition of the juveniles were determined as in vivo indicators. Juveniles grew significantly larger and faster when treated with a specific mix of isolates (MIX-B), compared with the control. The protein, lipid and carbohydrate concentrations were also significantly higher in oysters exposed to probiotic treatments, compared with the control and the antibiotic treatment. The selected microbiota showed probiotic proprieties for cultivating C. sikamea juveniles.
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comparative analysis of the reproductive strategy of lion s paw scallop Nodipecten subnodosus in baja california sur mexico
Latin American Journal of Aquatic Research, 2015Co-Authors: Marco A Angeldapa, Marcial Arellanomartinez, Bertha Patricia Ceballosvazquez, Hector Acostasalmon, Pedro E. SaucedoAbstract:The reproductive strategy of lion's paw scallop Nodipecten subnodosus was evaluated in a culturing system in Bahia Tortugas, Baja California Sur, Mexico during an annual cycle, comparing its response with data previously reported at other localities. High frequencies of ripe gonads throughout the year indicate that reproduction was continuous, with two main ripening/spawning events: July-September and December- March. A continuous breeding is also reported for the species in Bahia Magdalena, Bahia Juncalito, and Bahia de Los Angeles. These eutrophic areas are Biological Active Centers where gametogenesis appears to be regulated by the energy taken from recently ingested food following an opportunistic strategy. However, the digestive gland index decreased and the muscle indices increased during one of the breeding peaks, suggesting that some stored reserves are also used to sustain gametogenesis (conservative strategy) partially. High incidences of atretic oocytes are likely associated with atypical daily variations in water temperature from May through September (12 to 33°C), or with stressful conditions in the culturing system in summer. Despite this, the culturing system set in Bahia Tortugas appears beneficial for a continuous reproduction of N. subnodosus.
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Isolation and use of beneficial microbiota from the digestive tract of lions‐paw scallop Nodipecten subnodosus and winged pearl oyster Pteria sterna in oyster aquaculture
Aquaculture Research, 2015Co-Authors: Fernando Abasolo-pacheco, Ruben Araya, José Manuel Mazón-suástegui, Pedro E. Saucedo, Dariel Tovar-ramírez, Jesús M Ramírez‐orozco, Ángel I. Campa-córdovaAbstract:We isolated microbiota from the digestive tract of Nodipecten subnodosus and Pteria sterna and determined in vitro their haemolytic activity, antagonism against Vibrio spp., bacterial hydrophobicity, production of extracellular enzymes and molecular identification. Five bacterial strains were selected: RL5 and C3 (Lactobacillus spp.) and PB1-1, PB1-5 and PB1-6 (Bacillus spp.). The RL5 and C3 isolates showed antimicrobial activity against Vibrio spp. and the PB1-1, PB1-5 and PB1-6 isolates showed enzymatic activity for amylase, protease, lipase and cellulose; the C3 and PB1-5 isolates were highly hydrophobic. The selected strains of bacteria were tested in vivo as probiotics, together with a treatment of ampicillin and a control without bacteria on juvenile Kumamoto oysters Crassostrea sikamea. The strains were provided individually and as mixes of isolates. Survival, growth and biochemical composition of the juveniles were determined as in vivo indicators. Juveniles grew significantly larger and faster when treated with a specific mix of isolates (MIX-B), compared with the control. The protein, lipid and carbohydrate concentrations were also significantly higher in oysters exposed to probiotic treatments, compared with the control and the antibiotic treatment. The selected microbiota showed probiotic proprieties for cultivating C. sikamea juveniles.
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nutritional value of microalgae based diets for lions paw scallop Nodipecten subnodosus juveniles reared at different temperatures
Aquaculture, 2013Co-Authors: Pedro E. Saucedo, Hector Acostasalmon, Azucena Gonzalezjimenez, Jose Manuel Mazonsuastegui, Jose A RonsonpaulinAbstract:Abstract We studied the nutritional value of mono, binary, and ternary algal diets for the juvenile lions-paw scallop Nodipecten subnodosus reared at four temperatures: 16, 20, 24, and 28 °C. Live diets included one monoalgal (Isochrysis galbana), three binary (I. galbana + Pavlova salina, I. galbana + Chaetoceros muelleri, and P. salina + C. muelleri) and one ternary (I. galbana + P. salina + C. muelleri) mixtures of microalgae. Scallop responses were measured as survival, growth in shell height and wet and dry weight, and respiratory rate. Scallops grew significantly larger (6.81 mm; 39.75 g) and faster (0.13 mm d− 1; 1.11 g d− 1) and had higher respiratory rates (1326 mg O2 min− 1 mg− 1 dry weight of meat) when fed P. salina + C. muelleri and kept at 28 °C and 24 °C. In contrast, scallops grew significantly less (2.72 mm; 5.54 g) and slower (0.01 mm d− 1; 0.05 g d− 1), and had a lower respiratory rate (670 mg O2 min− 1 mg− 1 dry weight of meat) with a diet exclusively of I. galbana at 16 °C. Growth rates were significantly related to nutritional indices that include protein content. These results indicate the importance of this nutrient in juvenile growth, which is useful for optimizing culturing programs for this commercially important bivalve.
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growth survival and enzyme activity of lions paw scallop Nodipecten subnodosus spat treated with probiotics at the hatchery
Aquaculture Research, 2012Co-Authors: Andres Granadosamores, Angel I Campacordova, Ruben Araya, Jose Manuel Mazonsuastegui, Pedro E. SaucedoAbstract:Different types and concentrations of probiotics were evaluated for their effects on growth, survival and enzyme activity of hatchery-reared Nodipecten subnodosus spat. The treatments included (1) a mix of the microalgae Isochrysis galbana and Pavlova salina as the control group; (2) Diet 1 + a mix of Burkholderia cepacia and Pseudomonas aeruginosa; (3) Diet 1 + the commercial probiotic Epicin; and (4) Diet 1 + the commercial probiotic Bactosafe. Both commercial probiotics were tested at 3, 6 and 10 mg L 1 . Scallops receiving Bactosafe and Epicin at 3 and 6 mg L 1 grew ~30% faster and larger than in the control group. Regardless of the treatment, survival at the end was 98.7%. There was no relation between the activity of catalase and superoxide dismutase; however, significantly lower catalase levels occurred at increasing doses of Epicin; significant higher catalase levels occurred in the control group and mix of bacilli. Compared with the control group, lysozyme levels were significantly higher with the Epicin and Bactosafe treatments (3 and 6 mg L 1 ). More research needs to be conducted with probiotics to determine their real effects on the activity of antioxidant enzymes. We expect that treated scallops will increase their performance at the field.
Jose Manuel Mazonsuastegui - One of the best experts on this subject based on the ideXlab platform.
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isolation and use of beneficial microbiota from the digestive tract of lions paw scallop Nodipecten subnodosus and winged pearl oyster pteria sterna in oyster aquaculture
Aquaculture Research, 2016Co-Authors: Fernando Abasolopacheco, Ruben Araya, Dariel Tovarramirez, Jesus M Ramirezorozco, Jose Manuel Mazonsuastegui, Pedro E. Saucedo, Angel I CampacordovaAbstract:We isolated microbiota from the digestive tract of Nodipecten subnodosus and Pteria sterna and determined in vitro their haemolytic activity, antagonism against Vibrio spp., bacterial hydrophobicity, production of extracellular enzymes and molecular identification. Five bacterial strains were selected: RL5 and C3 (Lactobacillus spp.) and PB1-1, PB1-5 and PB1-6 (Bacillus spp.). The RL5 and C3 isolates showed antimicrobial activity against Vibrio spp. and the PB1-1, PB1-5 and PB1-6 isolates showed enzymatic activity for amylase, protease, lipase and cellulose; the C3 and PB1-5 isolates were highly hydrophobic. The selected strains of bacteria were tested in vivo as probiotics, together with a treatment of ampicillin and a control without bacteria on juvenile Kumamoto oysters Crassostrea sikamea. The strains were provided individually and as mixes of isolates. Survival, growth and biochemical composition of the juveniles were determined as in vivo indicators. Juveniles grew significantly larger and faster when treated with a specific mix of isolates (MIX-B), compared with the control. The protein, lipid and carbohydrate concentrations were also significantly higher in oysters exposed to probiotic treatments, compared with the control and the antibiotic treatment. The selected microbiota showed probiotic proprieties for cultivating C. sikamea juveniles.
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comparative growth and mortality of cultured lion s paw scallops Nodipecten subnodosus from gulf of california and pacific populations and their reciprocal transplants
Aquaculture Research, 2015Co-Authors: Volker Koch, Anna M Rengstorf, Marc H Taylor, Francisco Sinsel, Jose Manuel Mazonsuastegui, Matthias WolffAbstract:This study focused on the comparison of growth and mortality of Lion's Paw scallops Nodipecten subnodosus from Gulf of California and Pacific populations and their reciprocal transplants, cultured in suspended module and bottom culture in Baja California Sur, Mexico. Environmental parameters were monitored to determine site-specific differences and were correlated with scallops' population-specific responses. Significant differences in growth were found between sites, populations and grow-out methods. Growth performance of scallops originating from the Pacific population was lower and uniform between Pacific and Gulf culture sites, whereas Gulf scallops cultured at the Pacific site grew better. Growth and survival was higher in suspended than bottom culture, mainly due to depth-associated differences in food and oxygen supply. Increasing temperatures and decreasing food and oxygen during summer months resulted in depressed growth and elevated mortality. At the Pacific site, this was partially compensated by considerably higher food availability. Gulf scallops resisted high diurnal temperature changes at San Buto significantly better than Pacific scallops. They also survived generally better than the Pacific population, independent of site and grow-out method, indicating physiological adaptations to high temperatures and low food availability in their habitat. Consequently, the Gulf population appears to be more feasible for aquaculture operations in coastal lagoons with stressful conditions.
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nutritional value of microalgae based diets for lions paw scallop Nodipecten subnodosus juveniles reared at different temperatures
Aquaculture, 2013Co-Authors: Pedro E. Saucedo, Hector Acostasalmon, Azucena Gonzalezjimenez, Jose Manuel Mazonsuastegui, Jose A RonsonpaulinAbstract:Abstract We studied the nutritional value of mono, binary, and ternary algal diets for the juvenile lions-paw scallop Nodipecten subnodosus reared at four temperatures: 16, 20, 24, and 28 °C. Live diets included one monoalgal (Isochrysis galbana), three binary (I. galbana + Pavlova salina, I. galbana + Chaetoceros muelleri, and P. salina + C. muelleri) and one ternary (I. galbana + P. salina + C. muelleri) mixtures of microalgae. Scallop responses were measured as survival, growth in shell height and wet and dry weight, and respiratory rate. Scallops grew significantly larger (6.81 mm; 39.75 g) and faster (0.13 mm d− 1; 1.11 g d− 1) and had higher respiratory rates (1326 mg O2 min− 1 mg− 1 dry weight of meat) when fed P. salina + C. muelleri and kept at 28 °C and 24 °C. In contrast, scallops grew significantly less (2.72 mm; 5.54 g) and slower (0.01 mm d− 1; 0.05 g d− 1), and had a lower respiratory rate (670 mg O2 min− 1 mg− 1 dry weight of meat) with a diet exclusively of I. galbana at 16 °C. Growth rates were significantly related to nutritional indices that include protein content. These results indicate the importance of this nutrient in juvenile growth, which is useful for optimizing culturing programs for this commercially important bivalve.
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growth survival and enzyme activity of lions paw scallop Nodipecten subnodosus spat treated with probiotics at the hatchery
Aquaculture Research, 2012Co-Authors: Andres Granadosamores, Angel I Campacordova, Ruben Araya, Jose Manuel Mazonsuastegui, Pedro E. SaucedoAbstract:Different types and concentrations of probiotics were evaluated for their effects on growth, survival and enzyme activity of hatchery-reared Nodipecten subnodosus spat. The treatments included (1) a mix of the microalgae Isochrysis galbana and Pavlova salina as the control group; (2) Diet 1 + a mix of Burkholderia cepacia and Pseudomonas aeruginosa; (3) Diet 1 + the commercial probiotic Epicin; and (4) Diet 1 + the commercial probiotic Bactosafe. Both commercial probiotics were tested at 3, 6 and 10 mg L 1 . Scallops receiving Bactosafe and Epicin at 3 and 6 mg L 1 grew ~30% faster and larger than in the control group. Regardless of the treatment, survival at the end was 98.7%. There was no relation between the activity of catalase and superoxide dismutase; however, significantly lower catalase levels occurred at increasing doses of Epicin; significant higher catalase levels occurred in the control group and mix of bacilli. Compared with the control group, lysozyme levels were significantly higher with the Epicin and Bactosafe treatments (3 and 6 mg L 1 ). More research needs to be conducted with probiotics to determine their real effects on the activity of antioxidant enzymes. We expect that treated scallops will increase their performance at the field.
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positive results of enriching microalgae diets with cornstarch and wheat meal during hatchery rearing of lions s paw scallop Nodipecten subnodosus
2009Co-Authors: Jose Manuel Mazonsuastegui, M Osuna, P Ormart, Pedro E. Saucedo, Acuacultura RoblesAbstract:Introduction.The giant lion’s paw scallop Nodipecten subnodosus sustains an important fishery and commercial ventures in the Baja California peninsula (Mexico). It is the largest pectinid inhabiting tropical waters of the eastern Pacific Ocean and its growing acceptance within national and international markets offers a great opportunity for scientific research and technological development (OsunaGarcia et al., 2008), particularly for ensuring hatchery production of spat (MazonSuastegui et al., 2006). Hatchery rearing of bivalve molluscs requires constant provision of live microalgae as the main food source (Volkman and Brown, 2005). Microalgae, however, are expensive to produce and may account for 30 to 50% of hatchery operating costs (Jeffrey and Garland, 1987; Coutteau and Sorgeloos, 1992). Hence, great effort has been invested over recent decades in diets made of low-cost nutritional ingredients to partially substitute or complement the ratio of live microalgae (Mazon-Suastegui et al, 1988, 2008).