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Mohammad Ali Faramarzi - One of the best experts on this subject based on the ideXlab platform.
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Biotransformation of AD and ADD using Nostoc Muscorum
Iranian Journal of Pharmaceutical Research, 2010Co-Authors: M Tabatabei Yazdi, Mohammad Ali Faramarzi, Mohsen Amini, H Ghostinroudi, Younes GhasemiAbstract:The potential of microalgae for steroid transformation has been studied less than other microorganisms. They have been applied for the years in the treatment of environmental pollutions and preparation of nutrients. At the first time, Abul-Hajj and Qian indicated the conversion of androstendione (AD) to testosterone with eleven different strains of microalgae. Previtera’s group showed the transformations of progesterone and prednisolone in several microalgal cultures within four studies. In other investigations, steroid substrates were 5?-androstane-3, 17-dione, adrenosterone, androsta-1, 4-diene-3, 17-dione (ADD) and 17?-hydoxy-17?-methylandrosta-1, 4-dien-3-one. Hydrocortisone biotransformation using Nostoc Muscorum was also reported with Tabatabie group. In this study, the ability of Nostoc Muscorum, blue green algae, for biotransformation of AD and ADD were investigated. This potential has not been previously examined. The selected microalgae was isolated during a screening program from soil samples collected from paddy field of north of Iran. The fermentation media were included BG-11 medium together with AD or ADD 0.05%, separately, as steroid substrates. The experiment was illuminated continuously with fluorescent lamps at 40 µEm-2S-1, and incubated at a temperature of 25±2°C without shaking for ten days. The chloroform extract was loaded on silica gel plates with acetone/hexan (1:1) solvent system. The obtained fractions were further purified by re-chromatography, followed by crystallization. Then, the metabolites and unconverted substrates from each experiment were identified by melting points and spectral methods including 13C NMR, 1H NMR, FTIR and MS. The fermentation reaction of AD with Nostoc Muscorum led to accumulation of testosterone in the broth medium. 1-Dehydrotestosterone was the product of ADD bioconversion in the microalgal culture. In both cases, the bioconversion characteristics observed were 17-carbonyl reduction. Thus, the microorganism represented to act selectively at the ketone group on the D-ring of both androstane like steroids.
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influence of whole microalgal cell immobilization and organic solvent on the bioconversion of androst 4 en 3 17 dione to testosterone by Nostoc Muscorum
Journal of Molecular Catalysis B-enzymatic, 2010Co-Authors: Homeira Arabi, Tabatabaei M Yazdi, Mohammad Ali FaramarziAbstract:Abstract The use of free, immobilized and reused immobilized cells of the microalga Nostoc Muscorum was studied for bioconversion of androst-4-en-3,17-dione (AD) to testosterone in hexadecane. Among polymers such as agar, agarose, κ-carrageenan, polyacrylamide, polyvinyl alcohol, and sodium alginate that were examined for cell entrapment, sodium alginate with a concentration of 2% (w/v) proved to be the proper matrix for N. Muscorum cells immobilization. The bioconversion characteristics of immobilized whole algal cells at ranges of temperatures, substrate concentrations, and shaking speeds were studied followed by a comparison with those of free cells. The conditions were 30 °C, 0.5 g/L, and 100 rpm, respectively. The immobilized N. Muscorum showed higher yield (72 ± 2.3%) than the free form (24 ± 1.3%) at the mentioned conditions. The bioconversion yield did not decrease during reuse of immobilized cells and remained high even after 5 batches of bioreactions while Na-alginate 3% was used; however, reuse of alginate 2% beads did not give a satisfactory result.
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Bioconversion of codeine to semi-synthetic opiate derivatives by the cyanobacterium Nostoc Muscorum
World Journal of Microbiology and Biotechnology, 2010Co-Authors: Somayeh Niknam, Mohammad Ali Faramarzi, Mojtaba Tabatabaei Yazdi, Khosrou Abdi, Mohsen Amini, Hossein RastegarAbstract:Very limited studies have been done to investigate the algal biotransformation of codeine to its opioid derivatives. On the other hand, microalgae have been recently introduced as potential tools for green synthesis of various organic compounds. In the present work, the capability of biotransformation of codeine by a locally isolate strain of cyanobacterium, Nostoc Muscorum , was evaluated. Incubation of the whole cells of Nostoc Muscorum with codeine ( I ) under continuous light photoregime of 60 μmol photons/m^2s at 25°C for 5 days gave rise to four transformation products. The bioproducts were separated by gas chromatography and identified as 6-acetylcodeine ( II ), oxycodone ( III ), norcodeine ( IV ), morphine ( V ) and based on their mass spectra. Observed modifications included O-demethylation, N-demethylation, C6-acetylation, C14-hydroxilation, Δ^7-reduction, and C6-oxidation. The ability of N. Muscorum to convert codeine to oxycodone ( III ) represents an uncommon pattern of codeine metabolism in microorganisms that may be of industrial importance.
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application of organic mono phase and organic aqueous two liquid phase systems in microalgal conversion of androst 4 en 3 17 dione by Nostoc Muscorum
Biocatalysis and Biotransformation, 2009Co-Authors: Homeira Arabi, Mojtaba Tabatabaei Yazdi, Mohammad Ali FaramarziAbstract:Organic mono-phase and organic–aqueous two-phase systems were applied for 17-carbonyl reduction of androst-4-en-3,17-dione to testosterone by whole cells of the microalga Nostoc Muscorum (Nostocaceae). To investigate the correlation between solvent hydrophobicity and biotransformation yield in mono- and biphasic systems, a range of 16 organic solvents with log Poctanol values (logarithm of the solvent partition coefficient in the n-octanol/water system) between − 1.1 and 8.8 were examined. Organic solvents with log Poctanol values greater than 7, such as hexadecane and tetradecane, provided the best biocompatibility with the bioconversion by algal cells. The data also indicated that the highest yields were obtained using organic–aqueous (1:1, v/v) biphasic systems. The optimum volumetric phase ratio, reaction temperature and substrate concentration were 1:1, 30°C and 0.5 mg mL−1, respectively. Under the mentioned conditions a fourfold increase in biotransformation yield (from 7.8±2.3 to 33.4±1.8%) was obs...
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Nostoc Muscorum: a regioselective biocatalyst for 17-carbonyl reduction of androst-4-en-3,17-dione and androst-1,4-dien-3,17-dione
Annals of Microbiology, 2006Co-Authors: Mohammad Ali Faramarzi, Mohsen Amini, H Ghostinroudi, Younes Ghasemi, Mojtaba Tabatabaei Yazdi, Hoda Jahandar, Homeira ArabiAbstract:Nostoc Muscorum PTCC 1636 was examined for its ability to convert androst-4-en-3,17-dione (AD) and androst-1,4-dien-3,17-dione (ADD) to their 17-hydroxy related derivatives in BG-11 medium. Bioconversion procedures were carried out at 25 °C without shaking. The metabolites obtained were purified using chromatographic methods and characterized as testosterone and 1-dehydrotestosterone on the basis of their spectroscopic features. In both cases, the bioreaction characteristics observed were 17-carbonyl reduction.
Nirupama Mallick - One of the best experts on this subject based on the ideXlab platform.
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Carbon dioxide and poultry waste utilization for production of polyhydroxyalkanoate biopolymers by Nostoc Muscorum Agardh: a sustainable approach
Journal of Applied Phycology, 2016Co-Authors: Ranjana Bhati, Nirupama MallickAbstract:The new paradigm is to view wastes as resources for sustainable development. In this regard, the feasibility of poultry waste and CO2 utilization for cultivation of a filamentous nitrogen-fixing cyanobacterium, Nostoc Muscorum Agardh, was investigated for production polyhydroxyalkanoates, the biodegradable polymers. This cyanobacterium showed profound rise in biomass yield with up to 10 % CO2 supply in airstream with an aeration rate of 0.1 vvm. Maximum biomass yield of 1.12 g L−1 was recorded for 8 days incubation period, thus demonstrating a CO2 biofixation rate of 0.263 g L−1 day−1 at 10 % (v/v) CO2-enriched air. Poultry litter (PL) supplementation also had a positive impact on the biomass yield. The nutrient removal efficiency of N. Muscorum was reflected in the significant reduction in nutrient load of PL over the experimental period. A maximum poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid) [P(3HB-co-3HV)] copolymer yield of 774 mg L−1 (65 % of dry cell wt.), the value almost 11-fold higher than the control, was recorded in 10 g L−1 PL-supplemented cultures with 10 % CO2 supply under the optimized condition, thus demonstrating that N. Muscorum has good potential for CO2 biomitigation and poultry waste remediation while simultaneously producing eco-friendly polymers.
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Process optimization for poly(3-hydroxybutyrate-co-3-hydroxyvalerate) co-polymer production by Nostoc Muscorum
Biochemical Engineering Journal, 2007Co-Authors: Nirupama Mallick, Suneel Gupta, Bhabatarini Panda, Ramkrishna SenAbstract:Abstract This study aims at process optimization for poly(3-hydroxybutyrate-co-3-hydroxyvalarate) [P(3HB-co-3HV)] co-polymer production by Nostoc Muscorum with respect to variation in different parameters, viz. carbon concentration, time of incubation and pH using response surface methodology (RSM). Under pre-optimized condition, P(3HB-co-3HV) accumulation in N. Muscorum reached up to 28.2% (w/w) of dry cells (dcw) in presence of 0.2% acetate + 0.4% propionate, when incubated for 14 days at pH 8.5. A five-level four-factorial central composite design was employed to find out the interactions of four variables, viz. concentrations of acetate and propionate, pH and days of incubation. Using RSM, a second order polynomial equation was obtained by regression analysis. An optimum co-polymer yield of 31.4% (dcw) was achieved at a reduced level of carbons, i.e. 0.11% acetate and 0.08% propionate at pH 8.1 and an incubation period of 16 days. Thus after optimization, though the product yield was increased only by 11%, acetate and propionate requirements were reduced by 45 and 80%, respectively.
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poly β hydroxybutyrate accumulation in Nostoc Muscorum effects of metabolic inhibitors
Journal of Plant Physiology, 2007Co-Authors: Nirupama Mallick, Laxuman Sharma, Akhilesh Kumar SinghAbstract:Poly-beta-hydroxybutyrate (PHB) accumulation in Nostoc Muscorum was studied in presence of various metabolic inhibitors. Supplementation of 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) was found to suppress PHB accumulation in phosphate-limited N. Muscorum under photoautotrophic growth condition. PHB accumulation increased up to 21% and 17% from an initial PHB content of 8.5% of dry weight, respectively, under carbonylcyanide m-chlorophenylhydrazone (CCCP) and dicyclohexylcarbodiimide (DCCD) treatment, whereas 2,4 -dinitrophenol (DNP) supplementation depicted insignificant effect on PHB pool of the test cyanobacterium. Supplementation of l-methionine-dl-sulfoximine (MSX) and azaserine was also found to increase PHB accumulation in N(2) -fixing and NH(4)(+) -grown N. Muscorum, but not in NO(3)(-) -grown cells. The stimulatory action of monofluoroacetate on PHB accumulation was suppressed in presence of alpha-ketoglutarate and DCMU. Interestingly, 2,3 -butanedione supplementation was not only found inhibitory for accumulation of PHB in P-deficient, N-deficient and chemoheterotrophically grown N. Muscorum but suppression of PHB synthesis was also evident in control cultures in presence of 2,3 -butanedione. The possible mechanisms are discussed.
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process optimization for poly β hydroxybutyrate production in a nitrogen fixing cyanobacterium Nostoc Muscorum using response surface methodology
Bioresource Technology, 2007Co-Authors: Laxuman Sharma, Bhabatarini Panda, Akhilesh Kumar Singh, Nirupama MallickAbstract:Abstract A five-level-four-factor central composite rotary design was employed to find out the interactive effects of four variables, viz. concentrations of acetate, glucose and K2HPO4, and dark incubation period on poly-β-hydroxybutyrate (PHB) production in a N2-fixing cyanobacterium, Nostoc Muscorum. Acetate, glucose and dark incubation period exhibited positive impacts on PHB yield. Using response surface methodology (RSM), a second order polynomial equation was obtained by multiple regression analysis. A yield of 45.6% of dry cell weight (dcw) was achieved at reduced level of nutrients, i.e. 0.17% acetate, 0.16% glucose and 5 mg l−1 K2HPO4 at a dark incubation period of 95 h as compared to 41.6% PHB yield in 0.4% acetate, 0.4% glucose and 40 mg l−1 K2HPO4 at a dark incubation period of 168 h under single factor optimization strategy.
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studies on poly beta hydroxybutyrate synthase activity of Nostoc Muscorum
Journal of General and Applied Microbiology, 2006Co-Authors: Laxuman Sharma, Bhabatarini Panda, Akhilesh Kumar Singh, Nirupama MallickAbstract:This study compares the PHB synthase activity of Nostoc Muscorum, a N2-fixing cyanobacterium under control (grown in usual BG-11 medium), nitrogen (N) and phosphorus (P) deprivation and chemoheterotrophic conditions. Specific activity of PHB synthase did not depict significant variations in the latter three types of cultures, except for the control one, where a significantly lower activity was recorded. PHB synthase activity was detected only in the soluble fractions of both the control as well as cells incubated under chemoheterotrophic conditions. A Km of 80.2μM DL-β-hydroxybutyryl-CoA and Vmax of 197.5 nmol thiobenzoate (TNB) mg protein−1min−1 were observed for the enzyme. PHB synthase remained insensitive to acetyl-CoA, ATP, NADP, NADPH supplementation under in vitro condition. Addition of acetyl phosphate was found to activate the enzyme and the level of activation was dependent on the concentration of acetyl phosphate supplementation. Inhibition of PHB synthase in 2,3-butanedione supplemented cultures and reactivation following acetyl phosphate addition proved the post-translational control of acetyl phosphate over PHB synthase.
Mayashree B. Syiem - One of the best experts on this subject based on the ideXlab platform.
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Herbicide monuron mediated alterations in carbon and nitrogen fixation in the cyanobacterium Nostoc Muscorum Meg 1
Journal of Applied Phycology, 2021Co-Authors: Meguovilie Sachu, Balakyntiewshisha Lyngdoh Kynshi, Mayashree B. SyiemAbstract:This study aimed to investigate the impact of the herbicide monuron on various aspects of photosynthesis and diazotrophy in the cyanobacterium Nostoc Muscorum Meg 1 isolated from a rice field in Cherrapunji, Meghalaya, India. The consequences of monuron exposure on various photosynthetic pigments, functioning of PSII, RuBisCO, nitrogenase and glutamine synthetase enzyme activities, heterocyst frequency, and on production of proteins and carbohydrates in the cyanobacterium were studied in a range of monuron doses (20–100 ppm) to gauge the herbicide’s effect on CO_2 and N_2 fixation and on net biomass production. The total amount of D1 protein (the host for PSII complex), RuBisCO (the key enzyme for CO_2 fixation), nitrogenase (the enzyme responsible for the reduction of atmospheric nitrogen to ammonia), and ammonia assimilating enzyme glutamine synthetase (GS) contents under western blot analysis indicated interruption of new protein synthesis and breakdown of their existing enzyme molecules when exposed to higher monuron concentrations. All parameters studied showed enhanced expression under low dose monuron treatment (20 ppm) indicating a hormetic effect in the exposed organism. The expression of monuron toxicity on various parameters of CO_2 and N_2 fixation in a dose-dependent manner was immediately visible when cultures were treated with higher doses (40–100 ppm). Scanning and transmission electron microscopic studies further uncovered several undesirable changes in the morphology and ultrastructure of the organism due to herbicide treatment that could be correlated to compromised CO_2 and N_2 fixation.
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influence of calcium on cadmium uptake and toxicity to the cyanobacterium Nostoc Muscorum meg 1
Biotechnology Research and Innovation, 2019Co-Authors: Rabbul Ibne A Ahad, Mayashree B. SyiemAbstract:Abstract It is well known that Ca2+ interferes with uptake and expression of toxicity of Cd2+ in numerous organisms. This study demonstrated that the morphological alterations expressed in the cyanobacterium Nostoc Muscorum Meg 1 cells exposed to 0.5 mg L−1 Cd2+ for 7 days were prevented when 10 mg L−1 Ca2+ was included in the experimental set-up. The spectroscopic analysis confirmed a severe reduction in contents of chlorophyll a, phycocyanin, allophycocyanin, phycoerythrin, carotenoids and total protein at the end of 168 h (7 days) in presence of Cd2+; inclusion of Ca2+ significantly lessened these effects. Heterocyst frequency, nitrogenase and, glutamine synthetase activities were similarly improved in the presence of Ca2+. Uptake studies showed a reduction in 94% biosorption of supplied Cd2+ to 50% in the presence of Ca2+ indicating its competition with Cd2+ binding onto the cyanobacterial cell surfaces.
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copper and cadmium induced toxicity on the cyanobacterium Nostoc Muscorum meg 1 a comparative study
Eurasian Journal of Biosciences, 2018Co-Authors: Rabbul Ibne A Ahad, Mayashree B. SyiemAbstract:Seven days exposure to 10 μM Cu showed reduction in growth in terms of chlorophyll a and protein concentration by 38% and 21% in Nostoc Muscorum Meg 1. Same period of exposure to 10 μM Cd recorded reduction in these two parameters by 89% and 71%. Photosynthetic pigments carotenoids, phycocyanin, allophycocyanin and phycoerythrin were compromised > 75% in Cd as against ~ 14-21% in Cu. Nitrogenase and glutamine synthetase activities were down by ~ 75% in Cd, whereas the adverse effect of Cu was ~ 13%. The photosystem II activity and respiration were reduced by 44% and 53% in Cu but toxicity was more pronounced (> 70%) in Cd. Energy dispersion X-ray analysis established Cu and Cd binding to cell surface and Fourier Transform Infrared spectroscopic analysis determined the involvement of hydroxyl, carboxylic, carbonyl, nitro and amino groups in metal binding. Langmuir isotherm study established higher metal sorption capability with Qmax of 69.7 mg/g of biomass for Cd against 17.4 mg/g of biomass for Cu. Bright Field and Scanning Electron microscopy showed severity of Cd on filaments morphology compared to Cu. The cyanobacterium showed high efficiency (> 90%) for binding both metals making it a potential organism for research in the field of bioremediation.
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Zn2+ sequestration by Nostoc Muscorum: study of thermodynamics, equilibrium isotherms, and biosorption parameters for the metal
Environmental Monitoring and Assessment, 2017Co-Authors: Omega L. Diengdoh, Mayashree B. Syiem, Kannan Pakshirajan, A. N. RaiAbstract:Microbial biosorption has evolved as an effective strategy for heavy metal removal from contaminated waters. The common cyanobacterium Nostoc Muscorum isolated from the banks of a polluted river in Meghalaya, India, was tested for its potential to remove Zn2+ from aqueous solutions. Energy-dispersive X-ray (EDX) study verified Zn binding on the cyanobacterial biomass, and FTIR analysis revealed many negatively charged functional groups (hydroxyl, carbonyl, alcohol, amine, phosphoryl, sulfhydryl, and carboxyl) on the cell surface that aided in metal binding. Thermodynamic studies established the biosorption process to be energetically favorable with negative free energy change (−10.404, −10.599, and −10.796 kJ/mol at 298, 303, and 308 K, respectively). Sorption isotherm data fitted best in the Langmuir isotherm indicating monolayer nature of Zn sorption. The organism showed hyper-accumulation tendency towards Zn with a maximum sorption capacity as high as 2500 mg of Zn taken up per gram of biomass. The separation factor RL calculated from Langmuir isotherm ranged between 0 and 1 signifying favorable interaction between the cyanobacterial biomass and the Zn ions. Various experimental parameters, viz. pH, temperature, inoculum age and size, and shaking rate, influenced Zn biosorption. Optimized experimental conditions significantly enhanced the sorption percentage. Sorption was primarily a fast surface phenomenon in the beginning with internalization of zinc ions by the live cells on prolonged exposure.
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bioremoval of cu ii zn ii pb ii and cd ii by Nostoc Muscorum isolated from a coal mining site
Journal of Applied Phycology, 2015Co-Authors: Jayeeta Hazarika, Kannan Pakshirajan, Arindam Sinharoy, Mayashree B. SyiemAbstract:This study evaluated the potential of the cyanobacterium Nostoc Muscorum isolated from a mining environment for the removal of the heavy metals Cu(II), Zn(II), Pb(II) and Cd(II) from a constituent aqueous solution of different initial concentrations (5 to 50 mg L−1). Prior to the metal bioremoval experiments, the effect of three main culture parameters, i.e. inoculum volume, inoculum age and pH, on N. Muscorum growth was assessed and their levels optimized employing the Taguchi experimental design technique. The batch metal removal kinetics showed a maximum removal of Pb(II) (98 %) and Cu(II) (87.8 %), followed by Cd(II) (82 %) and Zn(II) (67.2 %) at 5 mg L−1 initial metal concentration. Furthermore, estimation of protein, carbohydrate and biomass contents of the metal-exposed N. Muscorum revealed that Zn(II) and Cd(II) are more toxic compared to Cu(II) at all initial metal concentrations, whereas it was more tolerant towards Pb(II).
Homeira Arabi - One of the best experts on this subject based on the ideXlab platform.
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influence of whole microalgal cell immobilization and organic solvent on the bioconversion of androst 4 en 3 17 dione to testosterone by Nostoc Muscorum
Journal of Molecular Catalysis B-enzymatic, 2010Co-Authors: Homeira Arabi, Tabatabaei M Yazdi, Mohammad Ali FaramarziAbstract:Abstract The use of free, immobilized and reused immobilized cells of the microalga Nostoc Muscorum was studied for bioconversion of androst-4-en-3,17-dione (AD) to testosterone in hexadecane. Among polymers such as agar, agarose, κ-carrageenan, polyacrylamide, polyvinyl alcohol, and sodium alginate that were examined for cell entrapment, sodium alginate with a concentration of 2% (w/v) proved to be the proper matrix for N. Muscorum cells immobilization. The bioconversion characteristics of immobilized whole algal cells at ranges of temperatures, substrate concentrations, and shaking speeds were studied followed by a comparison with those of free cells. The conditions were 30 °C, 0.5 g/L, and 100 rpm, respectively. The immobilized N. Muscorum showed higher yield (72 ± 2.3%) than the free form (24 ± 1.3%) at the mentioned conditions. The bioconversion yield did not decrease during reuse of immobilized cells and remained high even after 5 batches of bioreactions while Na-alginate 3% was used; however, reuse of alginate 2% beads did not give a satisfactory result.
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application of organic mono phase and organic aqueous two liquid phase systems in microalgal conversion of androst 4 en 3 17 dione by Nostoc Muscorum
Biocatalysis and Biotransformation, 2009Co-Authors: Homeira Arabi, Mojtaba Tabatabaei Yazdi, Mohammad Ali FaramarziAbstract:Organic mono-phase and organic–aqueous two-phase systems were applied for 17-carbonyl reduction of androst-4-en-3,17-dione to testosterone by whole cells of the microalga Nostoc Muscorum (Nostocaceae). To investigate the correlation between solvent hydrophobicity and biotransformation yield in mono- and biphasic systems, a range of 16 organic solvents with log Poctanol values (logarithm of the solvent partition coefficient in the n-octanol/water system) between − 1.1 and 8.8 were examined. Organic solvents with log Poctanol values greater than 7, such as hexadecane and tetradecane, provided the best biocompatibility with the bioconversion by algal cells. The data also indicated that the highest yields were obtained using organic–aqueous (1:1, v/v) biphasic systems. The optimum volumetric phase ratio, reaction temperature and substrate concentration were 1:1, 30°C and 0.5 mg mL−1, respectively. Under the mentioned conditions a fourfold increase in biotransformation yield (from 7.8±2.3 to 33.4±1.8%) was obs...
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Nostoc Muscorum: a regioselective biocatalyst for 17-carbonyl reduction of androst-4-en-3,17-dione and androst-1,4-dien-3,17-dione
Annals of Microbiology, 2006Co-Authors: Mohammad Ali Faramarzi, Mohsen Amini, H Ghostinroudi, Younes Ghasemi, Mojtaba Tabatabaei Yazdi, Hoda Jahandar, Homeira ArabiAbstract:Nostoc Muscorum PTCC 1636 was examined for its ability to convert androst-4-en-3,17-dione (AD) and androst-1,4-dien-3,17-dione (ADD) to their 17-hydroxy related derivatives in BG-11 medium. Bioconversion procedures were carried out at 25 °C without shaking. The metabolites obtained were purified using chromatographic methods and characterized as testosterone and 1-dehydrotestosterone on the basis of their spectroscopic features. In both cases, the bioreaction characteristics observed were 17-carbonyl reduction.
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biotransformation of hydrocortisone by a natural isolate of Nostoc Muscorum
Phytochemistry, 2004Co-Authors: Mojtaba Tabatabaei Yazdi, Mohammad Ali Faramarzi, Mohsen Amini, Younes Ghasemi, Homeira Arabi, Shadman Shokravi, Farzaneh Aziz MohseniAbstract:Hydrocortisone was converted in the culture of an isolated strain of the cyanobacterium Nostoc Muscorum PTCC 1636 into some androstane and pregnane derivatives. The microorganism was isolated during a screening program from soil samples collected from paddy fields of north of Iran. The bioproducts obtained were purified using chromatographic methods and identified as 11β-hydroxytestosterone, 11β-hydroxyandrost-4-en-3,17-dione and 11β,17α,20β,21-tetrahydroxypregn-4-en-3-one on the basis of their spectroscopic features.
Tasneem Fatma - One of the best experts on this subject based on the ideXlab platform.
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Toxicity of NSAID drug (paracetamol) to nontarget organism-Nostoc Muscorum.
Environmental science and pollution research international, 2020Co-Authors: Samreen Fatima, Nida Asif, Rakhshan Ahmad, Tasneem FatmaAbstract:Due to many folds increase in application of human and veterinary medicines, pharmaceuticals, a new category of pollutants, have emerged in our environment. They exist as residues in rivers, sewage effluents, streams, surface, ground, and potable water. Paracetamol (acetaminophen) is one such drug that is used as an antipyretic and analgesic medicine. It is a non-steroidal antiinflammatory drug (NSAID) and is easily available in the market because no medical prescription is necessary for its purchase and use. Paracetamol remains physiologically active even after their expiry period. Their detection in the environment in bioactive form has resulted in adverse effects on nontarget species. To determine the effect of paracetamol on aquatic photosynthetic organic (Cyanobacteria-Nostoc Muscorum), present study was performed. Paracetamol (25 mg/L, 50 mg/L, 75 mg/L, 100, 125, and 150 mg/L) exposure showed toxic responses on the test organism by generating oxidative stress (MDA, H2O2, O2.-). Paracetamol caused a significant decrease in growth of cyanobacteria and showed EC50 113.68 mg/L after the 6th day of treatment. Photosynthetic pigments (chlorophyll, carotenoid, and phycobiliprotein) decreased with paracetamol increase. Antioxidant enzymatic (SOD, CAT, APX, GST, and GR) and osmolyte (Proline) also increased with increase in paracetamol to counteract the oxidative stress.
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evaluation of antioxidant and anti proliferative efficacy of Nostoc Muscorum nccu 442
Biocatalysis and agricultural biotechnology, 2019Co-Authors: Durdana Yasin, Sabbir Ansari, Md Zafaryab, Nazia Ahmad, Noor Fatima Khan, Almaz Zaki, Meher Rizvi, Tasneem FatmaAbstract:Abstract Natural sources have always been the primary sources of bioactive principles. Cyanobacteria with its wide spectrum of metabolites represents a favourable source of compounds having medicinal properties like antioxidant, anti-proliferative etc. In this study we tested different organic extracts [Diethyl ether (DE), Dichloromethane (DM), Ethyl acetate (EA) and Heptane (HE) extracts] of cyanobacterium Nostoc Muscorum NCCU-442 for the same. Antioxidant activity was evaluated through DPPH and FRAP assay. Phenolic content was estimated using FCR reagent as phenolic compounds are the well-established antioxidants. MTT assay was used to estimate anti-proliferative activity against cervical cancer cell line, SiHa. DE extract was found to have the highest Phenolic content, i.e. 96.71 ± 2.41 mgGAE/g as well as most antioxidant effect in DPPH assay (IC50 =118 ± 0.34 µg/ml) and FRAP assay (EC1 =380.7 ± 2.43 µg/ml). The order of phenolic content of different extracts is: DE > EA> DM > HE. The order of antioxidant potential was same as the phenolic content. HE extract had the least antioxidant effect in the two assays (IC50 =686 ± 3.3 µg/ml and EC1 >10 mg/ml for DPPH and FRAP respectively). All the extracts showed anti-proliferative activity against the cancer cell line in the order: DE (IC50 = 70 ± 0.22 µg/ml) > HE (116.5 ± 0.53 µg/ml) > EA (205 ± 0.77 µg/ml) > DM (190 ± 0.45 µg/ml). During Gas Chromatography-Mass Spectrometry (GC-MS), bioactives like Benzofuranone derivatives, Myristoleic acid, Resorcinol, Citronellyl butyrates, hydroquinone, hexadecanoic acid, farnesol were identified in the organic extracts. Thus, our study indicated that Nostoc Muscorum NCCU-442 has an inherent capacity as a potential source of therapeutic compounds.
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FTIR spectrum of isolated PHB from Nostoc Muscorum NCCU- 442.
2016Co-Authors: Sabbir Ansari, Tasneem FatmaAbstract:FTIR spectrum of isolated PHB from Nostoc Muscorum NCCU- 442.
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1H NMR spectrum of isolated PHB from Nostoc Muscorum NCCU- 442.
2016Co-Authors: Sabbir Ansari, Tasneem FatmaAbstract:1H NMR spectrum of isolated PHB from Nostoc Muscorum NCCU- 442.
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Time course study of Nostoc Muscorum NCCU- 442 with respect to PHB (%) and growth (dry weight).
2016Co-Authors: Sabbir Ansari, Tasneem FatmaAbstract:Time course study of Nostoc Muscorum NCCU- 442 with respect to PHB (%) and growth (dry weight).
