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Katsuhiko Kondo - One of the best experts on this subject based on the ideXlab platform.

  • chromosome numbers of four species of Ochrosia sensu lato apocynaceae
    Chromosome Botany, 2008
    Co-Authors: Tsuyoshi Motohashi, Katsuhiko Kondo, Norikazu Tagashira, Goro Kokubugata, Mikio Aoyama
    Abstract:

    The chromosome numbers of four species of Ochrosia sensu lato (Apocynaceae) were reported: those of Neisosperma glomerata (2n=22) and Neisosperma citrodora (2n=23) were documented here for the first time, while those of Neisosperma oppositifolia (2n=22) and Ochrosia coccinea (2n=22) verified the previous counts.

  • molecular phylogeny of Ochrosia sensu lato apocynaceae based on rps16 intron and its sequence data supporting the inclusion of neisosperma
    Chromosome Botany, 2007
    Co-Authors: Katsuhiko Kondo
    Abstract:

    Molecular phylogeny of Ochrosia sensu lato (Ochrosia sensu stricto and Neisosperma) was investigated by maximum parsimony analysis of sequence data from chloroplast DNA (rps16 intron) and rps16 intron + Internal Transcribed Spacer (ITS). Nineteen species including two varieties (23 accessions) were defined as the ingroup, comprising ten species of Ochrosia s.str. and nine species of Neisosperma. For analysis using rps16 intron region, Rauvolfia sumatrana, R. verticillata and R. serpentina, Vinca major, and V. minor of the tribe Vinceae were used as the outgroup. The aims of this study are to evaluate the usefulness of rps16 intron to infer phylogenetic relationships within Ochrosia sensu lato and to confirm conclusions from previous analysis. In this study, rps16 region was proved to be sufficiently informative to infer phylogenetic relationships at infrageneric level. The strict consensus of most parsimonious trees produced from rps16 sequences data showed that Ochrosia species formed a monophyletic clade highly supported with 97% bootstrap value, whilst Neisosperma was paraphyletic. The Ochrosia clade was found to be nested within Neisosperma, with N.acuminata as a sister taxon to it. All Ochrosia and Neisosperma species were grouped into one clade. However, this clade was not well supported (only has 61% bootstrap value). Consequently, although the results does not contradict recognizing only one genus (Ochrosia sensu lato), but it is not convincing enough. The strict consensus tree produced from sequence data analysis using rps16 intron + ITS showed a more robust monophyletic clade of Ochrosia sensu lato, supported with 100% bootstrap value. Neisosperma - again - was found to be paraphyletic, with monophyletic clade of Ochrosia (strongly supported with 93% bootstrap value) nested within, suggesting the inclusion of Neisosperma into Ochrosia and recognition of only one genus. These findings supported our previous result, using ITS region.

  • molecular phylogeny of Ochrosia sensu lato apocynaceae based on its sequences data an evidence for the inclusion of neisosperma
    Chromosome Botany, 2007
    Co-Authors: Katsuhiko Kondo
    Abstract:

    Molecular phylogeny of Ochrosia sensu lato (Ochrosia sensu stricto and Neisosperma) was investigated by maximum parsimony analysis of sequence data from the internal transcribed spacer (ITS) of nuclear ribosomal DNA. Twenty-one species including two varieties (25 accessions), were defined as the ingroup. Three outgroup species were selected from tribe Vinceae (Kopsia arborea, K. flavida, and Rauvolfia serpentina). The ITS sequence data showed that Ochrosia s.l. is a highly defined monophyletic group (supported with 100% bootstrap value). As shown in the strict consensus tree, all Neisosperma species were placed at the basal part of the tree with N. glomerata and N. nakaiana as the basalmost taxa. All species belong to Ochrosia s.str. were grouped into one clade and nested within Neisosperma resulting in monophyletic Ochrosia s.str. and paraphyletic Neisosperma. This suggested that Neisosperma should be included into Ochrosia, and the two groups are treated as a single genus. The analysis also showed that subdivision of Ochrosia s.l. into sections based on fruit morphology (fibrous vs. massive and thick endocarp) is not supported.

  • monophyly of Ochrosia sensu lato apocynaceae evidence from its rps16 intron and morphological characters
    Chromosome Botany, 2007
    Co-Authors: Katsuhiko Kondo
    Abstract:

    A phylogenetic analysis of Ochrosia sensu lato (Ochrosia sensu stricto and Neisosperma) was performed based on 14 macromorphological characters scored from 19 species including two varieties (23 accessions), and three outgroup from genus Rauvolfia, i.e. R. sumatrana, R. verticillata and R. serpentina. For the same species, this data was analyzed together with molecular dataset from our previous studies. The result of total datasets analysis (of ITS, rps16 intron and macromorphological characters) confirmed the monophyly of Ochrosia sensu lato. Ochrosia sensu stricto appeared to be monophyletic. The clade nested within paraphyletic Neisosperma and together they form a strongly supported monophyletic clade of Ochrosia sensu lato. Most of the morphological characters used in this study were found to be homoplasious. The existence of fibrous-fruited group and cavity-fruited group was not supported, thus subdivision of Ochrosia into sections (or subgenera) based on this character as proposed by several authors cannot be applied.

B Gilbert - One of the best experts on this subject based on the ideXlab platform.

  • Chapter 9 The Alkaloids of Aspidosperma, Ochrosia, Pleiocarpa, Melodinus, and Related Genera
    The Alkaloids: Chemistry and Physiology, 2008
    Co-Authors: B Gilbert
    Abstract:

    Publisher Summary Meloscine and related alkaloids represent a completely novel group, whereas aspidodasycarpine is a new variation of the akuammigine type. A significant advance in structure determination is the introduction of high-resolution mass spectrometry, combined in some cases with the use of computer programs; this has been adapted to solve the structures of double alkaloids, such as pleiomutine. There has been considerable activity in the synthetic field and with the advances in the knowledge of biosynthesis, an increasing tendency is noted to model syntheses on the probable biogenetic route. New alkaloids and some of their derivatives together with plant sources and physical properties are provided in this chapter. Cotton effect that has no bearing on the C-2, C-12 stereochemistry is responsible for the observed sodium D-line rotation whose variations have been related to C-17 substitution. It is presumably dependent upon the orientation of the N a -acyl substituent. Number of alkaloids representing new variations in substitution of the aspidospermidine skeleton has been isolated. Among these are O -demethylpalosine and the 21-hydroxyderivative, N -acetyl- N -depropionylaspidoalbinol.

Jeanfrancois Butaud - One of the best experts on this subject based on the ideXlab platform.

  • a reassessment of marquesan Ochrosia and rauvolfia apocynaceae with two new combinations
    PhytoKeys, 2011
    Co-Authors: David H Lorence, Jeanfrancois Butaud
    Abstract:

    A reassessment of collections of Marquesan Apocynaceae assigned to the genera Neisosperma Raf., Ochrosia Juss., and Rauvolfia L. revealed that two nomenclatural changes are necessary: 1) transfer of Neisosperma brownii Fosberg & Sachet to the genus Ochrosia, as Ochrosia brownii (Fosberg & Sachet) Lorence & Butaud, comb. nov., and 2) transfer of Ochrosia nukuhivensis Fosberg & Sachet to Rauvolfia as Rauvolfia nukuhivensis (Fosberg & Sachet) Lorence & Butaud, comb. nov. As a result, two species each of Ochrosia and Rauvolfia are recognized from the Marquesas Islands, all endemic. Recent field work has yielded important new data on their distribution, habitat, and conservation status. It is recommended that all four species should be added the IUCN Red List at the Critically Endangered (CR) category.

  • The impacts of rats on the endangered native flora of French Polynesia (Pacific Islands): drivers of plant extinction or coup de grâce species?
    Biological Invasions, 2009
    Co-Authors: Jean-yves Meyer, Jeanfrancois Butaud
    Abstract:

    Although rats have clearly contributed to bird extinctions on islands, their role in plant extinctions is not as clear. Paleoenvironmental studies suggest rats were responsible for the demise of several island palm species. French Polynesia’s islands provide an opportunity to evaluate “modern” impacts of rats on native flora. Our study shows that 15 threatened taxa (nine families) are damaged by rats. All 12 subjected to seed predation are woody plants with large-seeded drupes. Three experience severe predation and recruitment depression ( Santalum insulare , Ochrosia tahitensis , Nesoluma nadeaudii ). Three-year monitoring of Polynesian sandalwood ( Santalum insulare ) populations in Tahiti during rat control suggested that over 99% of fruits were eaten before ripening. Seed predation on sandalwood appeared to be lower on islands without black rats Rattus rattus . Studies from Indo-Pacific islands document rat impact on at least 56 taxa (28 families). Certain families (Arecaceae, Elaeocarpaceae, Rubiaceae, Santalaceae, and Sapotaceae) are particularly vulnerable to seed predation. Other soft-barked trees (Araliaceae, Euphorbiaceae, and Malvaceae) suffer from stem or bark damages, especially during dry seasons. Although rats depress seedling recruitment and alter vegetation dynamics, no evidence demonstrates that they are solely responsible for current plant extinctions. Most of French Polynesia’s endangered species impacted by rats occur in severely degraded habitats. We therefore suggest that rats can be viewed more as coup de grâce species (i.e., that give the final stroke of death), rather than as main drivers of plant extinctions. More research is needed to clarify the impacts of rat species and their importance in plant population decline or demise.

Basset Olivé Joan - One of the best experts on this subject based on the ideXlab platform.

  • Disseny i síntesi de compostos policíclics que contenen el nucli indòlic. Avaluació de l’activitat antitumoral
    'Edicions de la Universitat de Barcelona', 2011
    Co-Authors: Basset Olivé Joan
    Abstract:

    L’el•lipticina, un compost aïllat originàriament d’Ocrosia elliptica Labill. (família Apocinaceae), posseeix una elevada activitat antiproliferativa. La seva estructura química consisteix en un esquelet tetracíclic format de carbazole fusionat amb una piridina. L’el•lipticina i els seus derivats exerceixen els seus efectes citotòxics i antineoplàstics a través d’un mecanisme multimodal d’acció biològica (inhibició de l’ADN-topoisomerasa II, intercalació a l’ADN, unió covalent a l’ADN i generació de radicals lliures citotòxics mitjançant reaccions d’oxidació-reducció). Prèviament en el grup de treball, s’han estudiat les 1,4-benzodioxinoisoquinolines dissenyades i sintetitzades com a anàlegs de l’el•lipticina. Tenint en compte els precedents bibliogràfics i la recerca duta a terme pel nostre grup en relació amb la preparació de compostos amb activitat anticancerígena, els objectius d’aquest treball van adreçats a la preparació i avaluació biològica de compostos policíclics que continguin el nucli de carbazole unit a un anell D pentacíclic i incorporin diferents cadenes alquíliques laterals. Es tracta d’estructures dissenyades a partir de l’el•lipticina principalment per farmacomodulació modulativa. Els intermediaris clau per a la obtenció d’aquestes estructures tetracícliques són diens de tipus furo[3,4-b]indole. En un primer lloc s’estableix una ruta sintètica a partir de l’àcid indole-2-carboxílic (ruta A, de 8 etapes i un rendiment global del 8-22%). D’aquesta ruta, cal destacar-ne la utilització d’N,N-dimetilhidrazida com a grup ortodirigent de metal•lació, que s’oxida a àcid mitjançant MnO2 en una etapa posterior. També es proposen d’altres rutes a partir del propi nucli indòlic (ruta B, de 5 etapes i un rendiment global del 31-76%). De la ruta B en destaquen la utilització de la funció acetal com a grup orto-dirigent de metal•lació i la utilització de resina àcida d’intercanvi iònic com a reactiu sòlid. D’altres rutes a partir de l’àcid indole-3-carboxílic o de l’indole-3-acetonitril no han resultat satisfactòries. El tractament dels diens obtinguts amb els dienòfils adients condueix als compostos tetracíclics desitjats. De la sèrie de compostos sintetitzats, s’han realitzat proves de citotoxicitat en les línies cel•lulars de leucèmia K562, càncer de pulmó NCIH460 i càncer de còlon HT-29. Per tal d’avaluar també la selectivitat dels compostos respecte a les cèl•lules no canceroses, s’ha realitzat un control en cèl•lules no canceroses de tipus HuDe (fibroblasts). Tots els productes obtinguts i els principals intermediaris s’han caracteritzat per tècniques d’RMN, IR i p.f. Es posa especial èmfasi en l’estudi de la reactivitat i en l’estabilitat dels intermediaris implicats i aïllats de les diferents rutes sintètiques. Finalment, durant una estada a la Universitat de Huddersfield (Anglaterra), s’ha realitzat la síntesi en fase sòlida de derivats indòlics, així com la posta al punt d’un mètode analític basat en les tècniques d’HPLC-Masses (ESI-ToF) per a la quantificació del pèptid fosfo-pRbING procedent de la reacció de fosforilació del pèptid pRbING mediada per CDK4/6. Aquest nou mètode constitueix una alternativa a la determinació de l’activitat de compostos inhibidors de CDK4/6 amb l’avantatge que no necessita de derivats de fòsfor marcats radioactivament i, per tant, ofereix menys riscos i major seguretat en el treball del laboratori.“Design and synthesis of polycyclic compounds that contain the indolyc nucleus. Evaluation of antitumor activity.” Ellipticine is a natural product isolated from Ochrosia elliptica with significant cytotoxic activity by several mechanisms of action. Its tetracyclic structure exhibits antineoplasic activity against several human cancer cell lines. Many syntheses have been reported by Gribble and col. and other authors, and its structure has been widely modified in order to obtain more efficacious, selective and less toxic new compounds. Our group had previously reported a series of ellipticine analogues including 1,4-benzodioxin nucleus. Herein, we present two new alternative pathways to prepare the intermediate isobenzofuroindole starting from either indole-2-carboxylic acid (8 steps, 8-22% overall yield) or 3-indolylalkanones (4 steps, 31-76% overall yield). Some interesting steps (involving protection of indole, directed ortho-metallation and cyclization) had been studied and developed in our laboratory. These methods lead us to the intermediate diene, which reacts with the corresponding dienophiles to obtain the desired compounds. These compounds were tested in cancer cell lines K-562 (leukaemia), NCI-H460 (lung) and HT-29 (colon), as well as in fibroblasts cell line HuDe to evaluate toxicity. All compounds were characterized by NMR, IR and m.p. techniques. Finally, over a PhD stage at the University of Huddersfield (England), some indole derivatives were synthesised by solid phase and a new HPLC-MS CDK4/6 inhibition test was designed. It was developed a 22-min HPLC-MS method to elute and quantify the phospho- pRbING product and the pRbING substrate from reaction samples containing buffer solution, ATP and CDK4/6. Peptide pRbING was efficiently quantified by HPLC-MS (ESI-ToF), within a range between 1 – 100 ppm and a detection limit as low as 3 pmol. This method should allow the evaluation of new CDK4/6 inhibitors

  • Disseny i síntesi de compostos policíclics que contenen el nucli indòlic: avaluació de l’activitat antitumoral
    'Edicions de la Universitat de Barcelona', 2011
    Co-Authors: Basset Olivé Joan
    Abstract:

    [cat] L’el·lipticina, un compost aïllat originàriament d’Ocrosia elliptica Labill. (família Apocinaceae), posseeix una elevada activitat antiproliferativa. La seva estructura química consisteix en un esquelet tetracíclic format de carbazole fusionat amb una piridina. L’el·lipticina i els seus derivats exerceixen els seus efectes citotòxics i antineoplàstics a través d’un mecanisme multimodal d’acció biològica (inhibició de l’ADN-topoisomerasa II, intercalació a l’ADN, unió covalent a l’ADN i generació de radicals lliures citotòxics mitjançant reaccions d’oxidació-reducció). Prèviament en el grup de treball, s’han estudiat les 1,4-benzodioxinoisoquinolines dissenyades i sintetitzades com a anàlegs de l’el·lipticina. Tenint en compte els precedents bibliogràfics i la recerca duta a terme pel nostre grup en relació amb la preparació de compostos amb activitat anticancerígena, els objectius d’aquest treball van adreçats a la preparació i avaluació biològica de compostos policíclics que continguin el nucli de carbazole unit a un anell D pentacíclic i incorporin diferents cadenes alquíliques laterals. Es tracta d’estructures dissenyades a partir de l’el·lipticina principalment per farmacomodulació modulativa. Els intermediaris clau per a la obtenció d’aquestes estructures tetracícliques són diens de tipus furo[3,4-b]indole. En un primer lloc s’estableix una ruta sintètica a partir de l’àcid indole-2-carboxílic (ruta A, de 8 etapes i un rendiment global del 8-22%). D’aquesta ruta, cal destacar-ne la utilització d’N,N-dimetilhidrazida com a grup ortodirigent de metal·lació, que s’oxida a àcid mitjançant MnO2 en una etapa posterior. També es proposen d’altres rutes a partir del propi nucli indòlic (ruta B, de 5 etapes i un rendiment global del 31-76%). De la ruta B en destaquen la utilització de la funció acetal com a grup orto-dirigent de metal·lació i la utilització de resina àcida d’intercanvi iònic com a reactiu sòlid. D’altres rutes a partir de l’àcid indole-3-carboxílic o de l’indole-3-acetonitril no han resultat satisfactòries. El tractament dels diens obtinguts amb els dienòfils adients condueix als compostos tetracíclics desitjats. De la sèrie de compostos sintetitzats, s’han realitzat proves de citotoxicitat en les línies cel·lulars de leucèmia K562, càncer de pulmó NCIH460 i càncer de còlon HT-29. Per tal d’avaluar també la selectivitat dels compostos respecte a les cèl·lules no canceroses, s’ha realitzat un control en cèl·lules no canceroses de tipus HuDe (fibroblasts). Tots els productes obtinguts i els principals intermediaris s’han caracteritzat per tècniques d’RMN, IR i p.f. Es posa especial èmfasi en l’estudi de la reactivitat i en l’estabilitat dels intermediaris implicats i aïllats de les diferents rutes sintètiques. Finalment, durant una estada a la Universitat de Huddersfield (Anglaterra), s’ha realitzat la síntesi en fase sòlida de derivats indòlics, així com la posta al punt d’un mètode analític basat en les tècniques d’HPLC-Masses (ESI-ToF) per a la quantificació del pèptid fosfo-pRbING procedent de la reacció de fosforilació del pèptid pRbING mediada per CDK4/6. Aquest nou mètode constitueix una alternativa a la determinació de l’activitat de compostos inhibidors de CDK4/6 amb l’avantatge que no necessita de derivats de fòsfor marcats radioactivament i, per tant, ofereix menys riscos i major seguretat en el treball del laboratori.[eng] “Design and synthesis of polycyclic compounds that contain the indolyc nucleus. Evaluation of antitumor activity.” Ellipticine is a natural product isolated from Ochrosia elliptica with significant cytotoxic activity by several mechanisms of action. Its tetracyclic structure exhibits antineoplasic activity against several human cancer cell lines. Many syntheses have been reported by Gribble and col. and other authors, and its structure has been widely modified in order to obtain more efficacious, selective and less toxic new compounds. Our group had previously reported a series of ellipticine analogues including 1,4-benzodioxin nucleus. Herein, we present two new alternative pathways to prepare the intermediate isobenzofuroindole starting from either indole-2-carboxylic acid (8 steps, 8-22% overall yield) or 3-indolylalkanones (4 steps, 31-76% overall yield). Some interesting steps (involving protection of indole, directed ortho-metallation and cyclization) had been studied and developed in our laboratory. These methods lead us to the intermediate diene, which reacts with the corresponding dienophiles to obtain the desired compounds. These compounds were tested in cancer cell lines K-562 (leukaemia), NCI-H460 (lung) and HT-29 (colon), as well as in fibroblasts cell line HuDe to evaluate toxicity. All compounds were characterized by NMR, IR and m.p. techniques. Finally, over a PhD stage at the University of Huddersfield (England), some indole derivatives were synthesised by solid phase and a new HPLC-MS CDK4/6 inhibition test was designed. It was developed a 22-min HPLC-MS method to elute and quantify the phospho- pRbING product and the pRbING substrate from reaction samples containing buffer solution, ATP and CDK4/6. Peptide pRbING was efficiently quantified by HPLC-MS (ESI-ToF), within a range between 1 – 100 ppm and a detection limit as low as 3 pmol. This method should allow the evaluation of new CDK4/6 inhibitors

Khalijah Awang - One of the best experts on this subject based on the ideXlab platform.

  • Evaluation of Green Corrosion Inhibition by Alkaloid Extracts of Ochrosia oppositifolia and Isoreserpiline against Mild Steel in 1 M HCl Medium
    2016
    Co-Authors: Ian Bothi Raja, Mehran Fadaeinasab, Ahmad Kaleem Qureshi, Afidah Abdul Rahim, Hasnah Osman, Marc Litaudon, Khalijah Awang
    Abstract:

    *S Supporting Information ABSTRACT: Alkaloid extracts of leaves (OOL) and bark (OOB) of Ochrosia oppositifolia, as well as isoreserpiline (ISR), the major alkaloid isolated from OOL and OOB, were investigated as potential corrosion inhibitors for mild steel (MS) in 1 M HCl medium. The inhibition properties of these phytoconstituents were studied using electrochemical techniques (potentiodynamic polarization measurements and electrochemical impedance spectroscopy, EIS) and scanning electron microscopy (SEM). The results indicated that these green inhibitors effectively reduced the corrosion rate. Polarization studies showed that these inhibitors decreased the corrosion current densities by a mixed-mode mechanism. The EIS data were analyzed by an equivalent circuit model for the electrode/electrolyte interface. SEM observations confirmed the existence of an adsorbed protective film of green inhibitors, and the adsorption was found to follow the Langmuir adsorption isotherm. FTIR and molecular modeling studies were also employed and revealed that the presence of ISR could be responsible for the corrosion inhibition potential of OOL and OOB. 1

  • evaluation of green corrosion inhibition by alkaloid extracts of Ochrosia oppositifolia and isoreserpiline against mild steel in 1 m hcl medium
    Industrial & Engineering Chemistry Research, 2013
    Co-Authors: Pandian Bothi Raja, Mehran Fadaeinasab, Ahmad Kaleem Qureshi, Afidah Abdul Rahim, Hasnah Osman, Marc Litaudon, Khalijah Awang
    Abstract:

    Alkaloid extracts of leaves (OOL) and bark (OOB) of Ochrosia oppositifolia, as well as isoreserpiline (ISR), the major alkaloid isolated from OOL and OOB, were investigated as potential corrosion inhibitors for mild steel (MS) in 1 M HCl medium. The inhibition properties of these phytoconstituents were studied using electrochemical techniques (potentiodynamic polarization measurements and electrochemical impedance spectroscopy, EIS) and scanning electron microscopy (SEM). The results indicated that these green inhibitors effectively reduced the corrosion rate. Polarization studies showed that these inhibitors decreased the corrosion current densities by a mixed-mode mechanism. The EIS data were analyzed by an equivalent circuit model for the electrode/electrolyte interface. SEM observations confirmed the existence of an adsorbed protective film of green inhibitors, and the adsorption was found to follow the Langmuir adsorption isotherm. FTIR and molecular modeling studies were also employed and revealed t...

  • Evaluation of Green Corrosion Inhibition by Alkaloid Extracts of Ochrosia oppositifolia and Isoreserpiline against Mild Steel in 1 M HCl Medium
    2013
    Co-Authors: Pandian Bothi Raja, Mehran Fadaeinasab, Ahmad Kaleem Qureshi, Afidah Abdul Rahim, Hasnah Osman, Marc Litaudon, Khalijah Awang
    Abstract:

    Alkaloid extracts of leaves (OOL) and bark (OOB) of Ochrosia oppositifolia, as well as isoreserpiline (ISR), the major alkaloid isolated from OOL and OOB, were investigated as potential corrosion inhibitors for mild steel (MS) in 1 M HCl medium. The inhibition properties of these phytoconstituents were studied using electrochemical techniques (potentiodynamic polarization measurements and electrochemical impedance spectroscopy, EIS) and scanning electron microscopy (SEM). The results indicated that these green inhibitors effectively reduced the corrosion rate. Polarization studies showed that these inhibitors decreased the corrosion current densities by a mixed-mode mechanism. The EIS data were analyzed by an equivalent circuit model for the electrode/electrolyte interface. SEM observations confirmed the existence of an adsorbed protective film of green inhibitors, and the adsorption was found to follow the Langmuir adsorption isotherm. FTIR and molecular modeling studies were also employed and revealed that the presence of ISR could be responsible for the corrosion inhibition potential of OOL and OOB

  • antioxidant and antlmicrobial activities of ferulic acid esters from Ochrosia oppositifolia
    Malaysian journal of science, 2011
    Co-Authors: Mehran Fadaei Nasab, Hamid A A Hadi, Ibrahim A Najmuldeen, Khalijah Awang, Ali Dadolahi Sohrab, Reza Farzin Ebrahimi, Arash Rafat, Arash Khorasan
    Abstract:

    Two ferulic acid esters namely (E)-methyl 3-(4’-hydroxy-3’,5’ dimethoxyphenyl) acrylate1 and (E)-methyl 18-((E)-3-(4’-hydroxy-3’methoxyphenyl)acryloyloxy)octadec-3-enoate ) 2, were isolated from Ochrosia oppositifolia. The compound 1 ,and 2 and the crude methanol and hexane extracts of Ochrosia oppositifolia showed a moderate in vitro antioxidant and antimicrobial activity. ( Keywords: Ochrosia oppositifolia, Apocynaceae, Ferulic acid esters, antimicrobial and antioxidant)