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Antonia Garrido Frenich - One of the best experts on this subject based on the ideXlab platform.
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Rapid and semiautomated method for the analysis of veterinary drug residues in honey based on turbulent-flow liquid chromatography coupled to ultrahigh-performance liquid chromatography-Orbitrap Mass Spectrometry (TFC-UHPLC-Orbitrap-MS).
Journal of agricultural and food chemistry, 2013Co-Authors: María Del Mar Aguilera-luiz, Roberto Romero-gonzález, Patricia Plaza-bolaños, José Luis Martínez Vidal, Antonia Garrido FrenichAbstract:A simple and rapid method is described for the determination of veterinary drug (VD) residues in honey samples using turbulent flow chromatography coupled to ultrahigh-performance liquid chromatography–Orbitrap Mass Spectrometry (TFC-UHPLC-Orbitrap-MS). Honey samples were diluted with an aqueous solution of Na2EDTA (0.1 M). Then, they were injected into the chromatographic system including a TFC column. Afterward, the analytes were transferred to an UHPLC analytical column, where they were determined by UHPLC-Orbitrap-MS. Mean recoveries were obtained at three concentration levels (5, 10, and 50 μg/kg), ranging from 68 to 121% for most compounds. Repeatability (intraday precision) and interday precision (expressed as relative standard deviation, RSD) were
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rapid and semiautomated method for the analysis of veterinary drug residues in honey based on turbulent flow liquid chromatography coupled to ultrahigh performance liquid chromatography Orbitrap Mass Spectrometry tfc uhplc Orbitrap ms
Journal of Agricultural and Food Chemistry, 2013Co-Authors: Maria Del Mar Aguileraluiz, José Luis Martínez Vidal, Roberto Romerogonzalez, Patricia Plazabolanos, Antonia Garrido FrenichAbstract:A simple and rapid method is described for the determination of veterinary drug (VD) residues in honey samples using turbulent flow chromatography coupled to ultrahigh-performance liquid chromatography–Orbitrap Mass Spectrometry (TFC-UHPLC-Orbitrap-MS). Honey samples were diluted with an aqueous solution of Na2EDTA (0.1 M). Then, they were injected into the chromatographic system including a TFC column. Afterward, the analytes were transferred to an UHPLC analytical column, where they were determined by UHPLC-Orbitrap-MS. Mean recoveries were obtained at three concentration levels (5, 10, and 50 μg/kg), ranging from 68 to 121% for most compounds. Repeatability (intraday precision) and interday precision (expressed as relative standard deviation, RSD) were <25% for most compounds. Limits of quantification (LOQs) ranged from 5 to 50 μg/kg and limits of identification (LOIs) from 0.1 to 50 μg/kg. The developed method was applied in honey samples, and it was fast and nonlaborious.
José Luis Martínez Vidal - One of the best experts on this subject based on the ideXlab platform.
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Rapid and semiautomated method for the analysis of veterinary drug residues in honey based on turbulent-flow liquid chromatography coupled to ultrahigh-performance liquid chromatography-Orbitrap Mass Spectrometry (TFC-UHPLC-Orbitrap-MS).
Journal of agricultural and food chemistry, 2013Co-Authors: María Del Mar Aguilera-luiz, Roberto Romero-gonzález, Patricia Plaza-bolaños, José Luis Martínez Vidal, Antonia Garrido FrenichAbstract:A simple and rapid method is described for the determination of veterinary drug (VD) residues in honey samples using turbulent flow chromatography coupled to ultrahigh-performance liquid chromatography–Orbitrap Mass Spectrometry (TFC-UHPLC-Orbitrap-MS). Honey samples were diluted with an aqueous solution of Na2EDTA (0.1 M). Then, they were injected into the chromatographic system including a TFC column. Afterward, the analytes were transferred to an UHPLC analytical column, where they were determined by UHPLC-Orbitrap-MS. Mean recoveries were obtained at three concentration levels (5, 10, and 50 μg/kg), ranging from 68 to 121% for most compounds. Repeatability (intraday precision) and interday precision (expressed as relative standard deviation, RSD) were
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rapid and semiautomated method for the analysis of veterinary drug residues in honey based on turbulent flow liquid chromatography coupled to ultrahigh performance liquid chromatography Orbitrap Mass Spectrometry tfc uhplc Orbitrap ms
Journal of Agricultural and Food Chemistry, 2013Co-Authors: Maria Del Mar Aguileraluiz, José Luis Martínez Vidal, Roberto Romerogonzalez, Patricia Plazabolanos, Antonia Garrido FrenichAbstract:A simple and rapid method is described for the determination of veterinary drug (VD) residues in honey samples using turbulent flow chromatography coupled to ultrahigh-performance liquid chromatography–Orbitrap Mass Spectrometry (TFC-UHPLC-Orbitrap-MS). Honey samples were diluted with an aqueous solution of Na2EDTA (0.1 M). Then, they were injected into the chromatographic system including a TFC column. Afterward, the analytes were transferred to an UHPLC analytical column, where they were determined by UHPLC-Orbitrap-MS. Mean recoveries were obtained at three concentration levels (5, 10, and 50 μg/kg), ranging from 68 to 121% for most compounds. Repeatability (intraday precision) and interday precision (expressed as relative standard deviation, RSD) were <25% for most compounds. Limits of quantification (LOQs) ranged from 5 to 50 μg/kg and limits of identification (LOIs) from 0.1 to 50 μg/kg. The developed method was applied in honey samples, and it was fast and nonlaborious.
Rosa Ma Lamuelaraventos - One of the best experts on this subject based on the ideXlab platform.
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comprehensive identification of walnut polyphenols by liquid chromatography coupled to linear ion trap Orbitrap Mass Spectrometry
Food Chemistry, 2014Co-Authors: Jorge Regueiro, Claudia Sanchezgonzalez, Maria Izquierdopulido, Rosa Ma Lamuelaraventos, Anna Vallverduqueralt, Jesus SimalgandaraAbstract:Epidemiologic studies and clinical trials have demonstrated consistent benefits of walnut consumption on coronary heart disease risk and other chronic diseases. Walnuts (Juglans regia L.) have been described previously as a rich source of polyphenols with a broad array of different structures. However, an accurate screening of its complete phenolic profile is still lacking. In the present work, liquid chromatography coupled with electrospray ionization hybrid linear trap quadrupole-Orbitrap Mass Spectrometry (LC–LTQ-Orbitrap) was applied for a comprehensive identification of phenolic compounds in walnuts. A total of 120 compounds, including hydrolysable and condensed tannins, flavonoids and phenolic acids were identified or tentatively identified on the base of their retention times, accurate Mass measurements and subsequent Mass fragmentation data, or by comparing with reference substances and literature. The peak area of each signal in Mass chromatograms was used to provide semiquantitative information for comparison purposes. The most abundant ions were observed for ellagitannins, ellagic acid and its derivatives. Furthermore, the high-resolution MS analysis revealed the presence of eight polyphenols that have never been reported in walnuts: stenophyllanin C, malabathrin A, eucalbanin A, cornusiin B, heterophylliin E, pterocarinin B, reginin A and alienanin B.
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improved characterization of tomato polyphenols using liquid chromatography electrospray ionization linear ion trap quadrupole Orbitrap Mass Spectrometry and liquid chromatography electrospray ionization tandem Mass Spectrometry
Rapid Communications in Mass Spectrometry, 2010Co-Authors: Rosa Ma Lamuelaraventos, Anna Vallverduqueralt, Olga Jauregui, Alexander Medinaremon, Cristina AndreslacuevaAbstract:Tomato (Lycopersicon esculentum Mill.) is the second most important fruit crop worldwide. Tomatoes are a key component in the Mediterranean diet, which is strongly associated with a reduced risk of chronic degenerative diseases. In this work, we use a combination of Mass Spectrometry (MS) techniques with negative ion detection, liquid chromatography/electrospray ionization linear ion trap quadrupole-Orbitrap-Mass Spectrometry (LC/ESI-LTQ-Orbitrap-MS) and liquid chromatography/electrospray ionization tandem Mass Spectrometry (LC/ESI-MS/MS) on a triple quadrupole, for the identification of the constituents of tomato samples. First, we tested for the presence of polyphenolic compounds through generic MS/MS experiments such as neutral loss and precursor ion scans on the triple quadrupole system. Confirmation of the compounds previously identified was accomplished by injection into the high-resolution system (LTQ-Orbitrap) using accurate Mass measurements in MS, MS 2 and MS 3 modes. In this way, 38 compounds were identified in tomato samples with very good Mass accuracy (<2 mDa), three of them, as far as we know, not previously reported in tomato samples. Copyright # 2010 John Wiley & Sons, Ltd.
Cong Kong - One of the best experts on this subject based on the ideXlab platform.
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Multi-Residue Screening of Pesticides in Aquaculture Waters through Ultra-High-Performance Liquid Chromatography-Q/Orbitrap Mass Spectrometry
Water, 2020Co-Authors: Shou-ying Wang, Essy Kouadio Fodjo, Cong KongAbstract:Pesticide residues in foodstuffs can lead to several undesirable effects. A simple and high-throughput targeted screening method analyzing multi-residue pesticide in aquaculture water based on ultra-high-performance liquid chromatography-Q/Orbitrap Mass Spectrometry (UHPLC-Q/Orbi MS) was developed and validated. In this technique, the peaks of the compound using precursor ions were recorded by the full scan, which was used for rough quantitative analysis with single point matrix matched calibration. The qualitative identification was performed following the stringent confirmation criteria with fragment ions, retention time, and an isotopic pattern. Additionally, solid-phase extraction with an HLB (Hydrophilic/Lipophilic Balanced) column was selected to enrich and separate target pesticides from water. The screening detection limit of 33 compounds are less than 2 ng·L−1, while 26 compounds range from 2 ng·L−1 to 10 ng·L−1, 19 compounds are at the range of 10–200 ng·L−1, and the other two compounds are 200 ng·L−1 and 1000 ng·L−1. Most of the recovery results were found to be between 60~130%. Finally, the method was successfully applied to the analysis of pesticide residues in 30 water samples from aquaculture environment in Shanghai, indicating its applicability in pesticide screening for environmental monitoring.
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Simultaneous determination of ten aminoglycoside antibiotics in aquatic feeds by high-performance liquid chromatography quadrupole-Orbitrap Mass Spectrometry with pass-through cleanup.
Chirality, 2019Co-Authors: Xiaoyi Lou, Yunyu Tang, Changling Fang, Cong Kong, Yongfu Shi, Dongmei Huang, Yaoguang Guo, Dongxue XiaoAbstract:A simple and sensitive method has been established based on pass-through cleanup and high-performance liquid chromatography quadrupole-Orbitrap Mass Spectrometry (HPLC-Q/Orbitrap MS) for the simultaneous determination of ten aminoglycosides (AGs) in aquatic feeds. The extraction solution and cleanup procedure had been optimized, and good sensitivity, accuracy, and precision were obtained. The calibration curves of AGs were linearity (R2 > 0.99) in the range of 2.0 to 200 μg/L (or 5.0 to 500 μg/L). The limits of detection of AGs were between 10 and 25 μg/kg. The recoveries of AGs ranged from 74.9% to 94.3%, and the intraday and interday relative standard deviations were less than 15%. Finally, this method was successfully applied to determine ten AGs in 30 aquatic feed samples. It might be the first time to use pass-through cleanup approach combined with HPLC-Q/Orbitrap MS method for AGs determination in aquatic feed samples.
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Multiclass screening of >200 pharmaceutical and other residues in aquatic foods by ultrahigh-performance liquid chromatography-quadrupole-Orbitrap Mass Spectrometry.
Analytical and bioanalytical chemistry, 2018Co-Authors: Cong Kong, Wang Yang, Yuanfei HuangAbstract:A quick screening method of more than 200 pharmaceutical and other residues in aquatic foods based on ultrahigh-performance liquid chromatography–quadrupole-Orbitrap Mass Spectrometry (UHPLC-Q/Orbitrap MS) was established. In this method, after the addition of 200 μL of 1 M EDTA-Na2, 2 g of each sample homogenate was extracted successively with 10 mL of acetonitrile and 10 mL of ethyl acetate. The extracts were combined, dried under nitrogen flow, and redissolved in 0.1% formic acid in acetonitrile/water (4:6, v/v) for analysis. The prepared samples were analyzed by UHPLC- Q/Orbitrap MS system in Full MS/ddMS2 (full-scan data-dependent MS/MS) mode. Compound identification was performed through comparison of the sample data with the database for standard chemicals, including the retention time, precursor ion, product ions, and isotope pattern for all 206 compounds. Five different aquatic food matrices (carp, shrimp, crab, eel, and mussel) spiked with the analytes at 1, 10, and 50 ng/g were evaluated to assess recoveries, precision, matrix effects, stability, and detection limits using the method. UHPLC analyses required 25 min, and 178–200 analytes met identification criteria at 50 ng/g depending on the matrix. Furthermore, practical application of this method for real samples displayed strong screening capability.
Vadims Bartkevics - One of the best experts on this subject based on the ideXlab platform.
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Development and Validation of New Ultra-High-Performance Liquid Chromatography-Hybrid Quadrupole-Orbitrap Mass Spectrometry Method for Determination of Melatonin in Fruits.
Journal of chromatographic science, 2016Co-Authors: Ingars Reinholds, Jekaterina Rjabova, Iveta Pugajeva, Vitalijs Radenkovs, Vadims BartkevicsAbstract:A new reliable analytical method based on ultra-high-performance liquid chromatography coupled with hybrid quadrupole-Orbitrap Mass Spectrometry was developed for the analysis of melatonin (MEL) in food products. In-house validation was performed using deuterated melatonin (MEL-d4) as an internal standard to ensure method selectivity and accuracy and to evaluate the efficiency of a robust ethyl acetate extraction technique used for sample preparation. The analysis of 18 tart cherry varieties and 28 tomato varieties was performed at optimized conditions. The method was linear (R(2)> 0.99) over the concentration range of 5-200 pg/g. A very low limit of quantification (10 pg/g) was provided for both analyzed matrices. The determined average recoveries (102 and 110%) and the values of intraday repeatability (6.30 and 10.9%) for cherry and tomato matrices, respectively, indicated a good accuracy and precision. The elaborated procedure proved the absence of MEL in any of tart cherries (
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the development and validation of a rapid method for the determination of antimicrobial agent residues in milk and meat using ultra performance liquid chromatography coupled to quadrupole Orbitrap Mass Spectrometry
Journal of Pharmaceutical and Biomedical Analysis, 2015Co-Authors: Guntis Cepurnieks, Jekaterina Rjabova, Dzintars Zacs, Vadims BartkevicsAbstract:Abstract A new multi-class method has been developed for the identification and quantification of the residues of 26 antibiotics from different classes (sulfonamides, macrolides, tetracyclines, penicillins, and quinolones) in milk and meat by ultra performance liquid chromatography coupled to hybrid quadrupole – high resolution Orbitrap Mass Spectrometry (UPLC–qOrbitrap). The sample preparation included extraction of two analytical portions with acetonitrile and 5% trichloroacetic acid, respectively, followed by centrifugation and filtration. The method was validated over three days at 50% of MRL (maximum residue limit) set in the European Union. Experiments on spiked meat and milk samples showed that the average recovery of the antibiotics ranged from 83% to 112%, and the coefficients of variation were between 8.9% and 39%.
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The development and validation of a rapid method for the determination of antimicrobial agent residues in milk and meat using ultra performance liquid chromatography coupled to quadrupole – Orbitrap Mass Spectrometry
Journal of pharmaceutical and biomedical analysis, 2014Co-Authors: Guntis Cepurnieks, Jekaterina Rjabova, Dzintars Zacs, Vadims BartkevicsAbstract:A new multi-class method has been developed for the identification and quantification of the residues of 26 antibiotics from different classes (sulfonamides, macrolides, tetracyclines, penicillins, and quinolones) in milk and meat by ultra performance liquid chromatography coupled to hybrid quadrupole - high resolution Orbitrap Mass Spectrometry (UPLC-qOrbitrap). The sample preparation included extraction of two analytical portions with acetonitrile and 5% trichloroacetic acid, respectively, followed by centrifugation and filtration. The method was validated over three days at 50% of MRL (maximum residue limit) set in the European Union. Experiments on spiked meat and milk samples showed that the average recovery of the antibiotics ranged from 83% to 112%, and the coefficients of variation were between 8.9% and 39%.