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Jinjun Wang - One of the best experts on this subject based on the ideXlab platform.
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Molecular characterization of ecdysis triggering hormone and its receptor in citrus red mite (Panonychus citri).
Comparative biochemistry and physiology. Part A Molecular & integrative physiology, 2019Co-Authors: Lin Zhu, Jinjun Wang, Wei Zhang, Qin-zhe Sun, Guy Smagghe, Hong-bo JiangAbstract:Abstract Neuropeptide ecdysis triggering hormone (ETH) plays crucial roles in invertebrates by activating a G protein-coupled receptor (GPCR), the ecdysis triggering hormone receptor (ETHR), which has been intensively investigated in Hexapoda. However, the molecular characterization of ETH and ETHR in Chelicerata remains unknown. In this study, we identified and characterized the full-length cDNA of ETH and ETHR in citrus red mite Panonychus citri (McGregor). Phylogenetic analysis indicated that PcETHR was closely related to the insect ETHR subtype B. A calcium mobilization-based functional assay showed that PcETH activated the PcETHR in a dose-dependent manner. Furthermore, a sharp upregulation of PcETH before ecdysis was observed by quantitative real-time PCR (qRT-PCR), and it revealed a tight correlation of ETH signaling and the molting process. These are the first insights into the molecular characterization of ETH and its receptor in mites as P. citri. Our work provides basic information of the ETH and ETHR in P. citri and lays a solid foundation for further physiological functions to better understand the ETH signaling system in mites.
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involvement of three esterase genes from Panonychus citri mcgregor in fenpropathrin resistance
International Journal of Molecular Sciences, 2016Co-Authors: Xiaomin Shen, Chongyu Liao, Xueping Lu, Zhe Wang, Jinjun WangAbstract:The citrus red mite, Panonychus citri (McGregor), is a major citrus pest with a worldwide distribution and an extensive record of pesticide resistance. However, the underlying molecular mechanism associated with fenpropathrin resistance in this species have not yet been reported. In this study, synergist triphenyl phosphate (TPP) dramatically increased the toxicity of fenpropathrin, suggesting involvement of carboxylesterases (CarEs) in the metabolic detoxification of this insecticide. The subsequent spatiotemporal expression pattern analysis of PcE1, PcE7 and PcE9 showed that three CarEs genes were all over-expressed after insecticide exposure and higher transcripts levels were observed in different field resistant strains of P. citri. Heterologous expression combined with 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetra-zolium bromide (MTT) cytotoxicity assay in Spodoptera frugiperda (Sf9) cells revealed that PcE1-, PcE7- or PcE9-expressing cells showed significantly higher cytoprotective capability than parental Sf9 cells against fenpropathrin, demonstrating that PcEs probably detoxify fenpropathrin. Moreover, gene silencing through the method of leaf-mediated dsRNA feeding followed by insecticide bioassay increased the mortalities of fenpropathrin-treated mites by 31% (PcE1), 27% (PcE7) and 22% (PcE9), respectively, after individual PcE gene dsRNA treatment. In conclusion, this study provides evidence that PcE1, PcE7 and PcE9 are functional genes mediated in fenpropathrin resistance in P. citri and enrich molecular understanding of CarEs during the resistance development of the mite.
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functional analysis of a chitinase gene during the larval nymph transition in Panonychus citri by rna interference
Experimental and Applied Acarology, 2016Co-Authors: Xiaomin Shen, Chongyu Liao, Ying-cai Feng, Tianbo Ding, Rui Zhong, Jinjun WangAbstract:Chitinases are hydrolytic enzymes that are required for chitin degradation and reconstruction in arthropods. In this study, we report a cDNA sequence encoding a putative chitinase (PcCht1) from the citrus red mite, Panonychus citri. The PcCht1 (564 aa) possessed a signal peptide, a conserver domain, and a chitin-binding domain. Structural and phylogenetic analyses found that PcCht1 had high sequence similarity to chitinases in Tetranychus urticae. Real-time quantitative PCR analyses showed that the transcript levels of PcCht1 peaked periodically in larval and nymph stages. Moreover, significant increase of PcCht1 transcript level in the larvae was observed upon the exposure of diflubenzuron. In contrast, exposures of the larvae to diflubenzuron resulted in the decreased chitin content. Furthermore, through a feeding-based RNA interference approach, we were able to reduce the PcCht1 transcript level by 59.7 % in the larvae, and consequently the treated larvae showed a very low molting rate compared with the control. Our results expanded the understanding of the important role of PcCht1 in the growth and development of P. citri.
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Regulation of three isoforms of SOD gene by environmental stresses in citrus red mite, Panonychus citri.
Experimental & applied acarology, 2015Co-Authors: Ying-cai Feng, Chongyu Liao, Wen-kai Xia, Xuan-zhao Jiang, Feng Shang, Guo-rui Yuan, Jinjun WangAbstract:Superoxide dismutase (SOD) is a family of enzymes with multiple isoforms that possess antioxidative abilities in response to environmental stresses. Panonychus citri is one of the most important pest mites and has a global distribution. In this study, three distinct isoforms of SOD were cloned from P. citri and identified as cytoplasmic Cu-ZnSOD (PcSOD1), extracellular Cu-ZnSOD (PcSOD2), and mitochondrial MnSOD (PcSOD3). mRNA expression level analysis showed that all three isoforms were up-regulated significantly after exposure to the acaricide abamectin and to UV-B ultraviolet irradiation. In particular, PcSOD3 was up-regulated under almost all environmental stresses tested. The fold change of PcSOD3 expression was significantly higher than those of the two Cu-ZnSOD isoforms. Taken together, the results indicate that abamectin and UV-B can induce transcripts of all three SOD isoforms in P. citri. Furthermore, PcSOD3 seems to play a more important role in P. citri tolerance to oxidative stress.
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molecular characterization of two carboxylesterase genes of the citrus red mite Panonychus citri acari tetranychidae
Archives of Insect Biochemistry and Physiology, 2013Co-Authors: Kun Zhang, Tianbo Ding, Wei Dou, Jin-zhi Niu, Jinjun WangAbstract:The citrus red mite, Panonychus citri, is known for its ability to rapidly evolve resistance to insecticides/acaricides and to adapt to hosts that produce toxins. To get better insight into the detoxification mechanism of P. citri, two carboxylesterase (CarE) genes, PCE1 and PCE2, were isolated and characterized. PCE1 and PCE2 contained open reading frames of 1,653 and 1,392 nucleotides, encoding proteins of 550 and 463 amino acid residues, respectively. Phylogenetic analyses showed that PCE1 and PCE2 were most closely related to the CarE genes from other phytophagous mites. The transcriptional profiles of two CarE genes among developmental stages (egg, larva, nymph, adult female, and adult male), after exposing to four acaricides (avermectin, azocyclotin, pyridaben, and spirodiclofen) and acid rain were investigated using real-time quantitative PCR (qPCR). The results showed that during development, PCE1 was highly expressed at the egg stage, whereas PCE2 was abundantly expressed at the adult stage of males. The expression levels of PCE1 were highly induced upon exposure to acaricides and acid rain. On the other hand, the expression levels of PCE2 were increased after treatment with avermectin and pyridaben. These results suggest that PCE1 and PCE2 may have distinct roles in different developmental stages and participate in the detoxification of acaricides.
Wei Dou - One of the best experts on this subject based on the ideXlab platform.
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molecular characterization of two carboxylesterase genes of the citrus red mite Panonychus citri acari tetranychidae
Archives of Insect Biochemistry and Physiology, 2013Co-Authors: Kun Zhang, Tianbo Ding, Wei Dou, Jin-zhi Niu, Jinjun WangAbstract:The citrus red mite, Panonychus citri, is known for its ability to rapidly evolve resistance to insecticides/acaricides and to adapt to hosts that produce toxins. To get better insight into the detoxification mechanism of P. citri, two carboxylesterase (CarE) genes, PCE1 and PCE2, were isolated and characterized. PCE1 and PCE2 contained open reading frames of 1,653 and 1,392 nucleotides, encoding proteins of 550 and 463 amino acid residues, respectively. Phylogenetic analyses showed that PCE1 and PCE2 were most closely related to the CarE genes from other phytophagous mites. The transcriptional profiles of two CarE genes among developmental stages (egg, larva, nymph, adult female, and adult male), after exposing to four acaricides (avermectin, azocyclotin, pyridaben, and spirodiclofen) and acid rain were investigated using real-time quantitative PCR (qPCR). The results showed that during development, PCE1 was highly expressed at the egg stage, whereas PCE2 was abundantly expressed at the adult stage of males. The expression levels of PCE1 were highly induced upon exposure to acaricides and acid rain. On the other hand, the expression levels of PCE2 were increased after treatment with avermectin and pyridaben. These results suggest that PCE1 and PCE2 may have distinct roles in different developmental stages and participate in the detoxification of acaricides.
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transcriptome analysis of the citrus red mite Panonychus citri and its gene expression by exposure to insecticide acaricide
Insect Molecular Biology, 2012Co-Authors: J Z Niu, Guy Smagghe, Wei Dou, Guanwang Shen, Kui Zhang, T B Ding, Jinjun WangAbstract:The citrus red mite, Panonychus citri, is known for its ability rapidly to evolve resistance to insecticides/acaricides and to adapt to hosts that produce toxins. In this study, we constructed an unprecedented four gigabase pair transcriptome of P. citri, which was assembled into 64 149 unique transcripts, the functions of which were annotated by five public databases. A total of 116 unique transcripts were identified as representatives of potential involvement in the detoxification of xenobiotics. Genes recorded to encoding insecticide/acaricide target proteins were also obtained from the P. citri transcriptome. In order to explore novel candidate genes potentially involved in the pesticide detoxification of P. citri, we also constructed digital gene expression libraries of short-term transcriptome responses of P. citri to pesticides, which resulted in the identification of 120 unique transcripts potentially associated with insecticide/acaricide detoxification. Our study will facilitate molecular research on pesticide resistance in citrus red mites, as well as in other phytophagous mites.
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Transcriptome analysis of the citrus red mite, Panonychus citri, and its gene expression by exposure to insecticide/acaricide.
Insect molecular biology, 2012Co-Authors: Wei Dou, Guy Smagghe, Guanwang Shen, Kui Zhang, Jinjun WangAbstract:The citrus red mite, Panonychus citri, is known for its ability rapidly to evolve resistance to insecticides/acaricides and to adapt to hosts that produce toxins. In this study, we constructed an unprecedented four gigabase pair transcriptome of P. citri, which was assembled into 64 149 unique transcripts, the functions of which were annotated by five public databases. A total of 116 unique transcripts were identified as representatives of potential involvement in the detoxification of xenobiotics. Genes recorded to encoding insecticide/acaricide target proteins were also obtained from the P. citri transcriptome. In order to explore novel candidate genes potentially involved in the pesticide detoxification of P. citri, we also constructed digital gene expression libraries of short-term transcriptome responses of P. citri to pesticides, which resulted in the identification of 120 unique transcripts potentially associated with insecticide/acaricide detoxification. Our study will facilitate molecular research on pesticide resistance in citrus red mites, as well as in other phytophagous mites.
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Susceptibility and Activity of Glutathione S-Transferases in Nine Field Populations of Panonychus citri (Acari: Tetranychidae) to Pyridaben and Azocyclotin
Florida Entomologist, 2011Co-Authors: Jin-zhi Niu, Wei Dou, Guo-ying Liu, Jinjun WangAbstract:ABSTRACT Nine field collected populations of Panonychus citri from Chinese citrus orchards were assayed for susceptibility to pyridaben and the alternative acaricide azocyclotin and activity of glutathione S-transferases (GSTs). The results showed that populations from Pujiang, Wanzhou, and Pengshan exhibited a low level of sensitivity to pyridaben, but demonstrated a high level of sensitivity to azocyclotin. The correlation coefficient between GSTs activities and the LC50 of pyridaben was r = 0.93 while the correlation coefficient between GSTs activities and the LC50 of azocyclotin was r = 0.03. The Vmax value of CDNB (1-chloro-2, 4-dinitrobenzene) in populations from Beibei, Jintang, Pengshan, Wanzhou, and Zhongxian exhibited a: 2.5-, 11.6-, 7.0-, 5.1-, and 6.4-fold increase in resistance, respectively, relative to the pyridaben susceptible population. In addition, azocyclotin was the most sensitive inhibitor of the GSTs compared with the EA (ethacrynic acid) and pyridaben, based on the values for I 50....
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Susceptibility and esterase activity in citrus red mite Panonychus citri (McGregor) (Acari: Tetranychidae) after selection with phoxim
International Journal of Acarology, 2009Co-Authors: Zhi‐yong Chen, Chun Ran, Ling Zhang, Wei Dou, Jinjun WangAbstract:Changes in susceptibility and esterase activity were measured in the citrus red mite, Panonychus citri (McGregor), which was repeatedly exposed to phoxim, an organophosphorus acaricide, for 15 repeated treatments as potential selection at the 50% lethal concentration. A reference or non‐selected strain (SS) was not exposed to any acaricides throughout the experiments. After 15 repeated phoxim selection events, the resistance ratio increased to 18.6‐fold and this strain was considered resistant to phoxim and named Ph‐R. The activity and specific activity of carboxylesterase (CarE) and acetylcholinesterase (AChE) in Ph‐R were all significantly higher than those in SS. The Michaelis–Menten constants (K m) of both CarE and AChE in Ph‐R were significantly higher than those in SS, suggesting decreased affinities of the enzymes in Ph‐R to the corresponding substrates. In addition, in vitro inhibition of phoxim against CarE of the mite showed that CarE from SS was more sensitive than that from Ph‐R, and an in vit...
Tong Wen - One of the best experts on this subject based on the ideXlab platform.
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The spatial distribution pattern and sampling technique of Panonychus citri McGregor
Entomological Journal of East China, 2003Co-Authors: Tong WenAbstract:The spatial distribution pattern was analysed and the sampling technique was studied for Panonychus citri McGregor by Iwao's model. The results showed that the spatial distribution pattern of Panonychus citri belonged to the aggregated, and the sampling was on the leave of the up, mid and down positions in fields. However, the number of theoretical sampling was provided for evaluation of Panonychus citri under different density.
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Study on the spatial distribution and sampling techniques of Panonychus citri Mcgregor
2003Co-Authors: Tong WenAbstract:The spatial distributions of Panonychus citri Mcgregor were analyzed by use of α,βand six cluster indicators of the Iwao M X model. Results indicated that the spatial distributions of Panonychus citri Mcgregor were cluster. The theoretical numbers of sampling in the condition of different densities of Panonychus citri Mcgregor were deduced based on this research.
Guy Smagghe - One of the best experts on this subject based on the ideXlab platform.
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Molecular characterization of ecdysis triggering hormone and its receptor in citrus red mite (Panonychus citri).
Comparative biochemistry and physiology. Part A Molecular & integrative physiology, 2019Co-Authors: Lin Zhu, Jinjun Wang, Wei Zhang, Qin-zhe Sun, Guy Smagghe, Hong-bo JiangAbstract:Abstract Neuropeptide ecdysis triggering hormone (ETH) plays crucial roles in invertebrates by activating a G protein-coupled receptor (GPCR), the ecdysis triggering hormone receptor (ETHR), which has been intensively investigated in Hexapoda. However, the molecular characterization of ETH and ETHR in Chelicerata remains unknown. In this study, we identified and characterized the full-length cDNA of ETH and ETHR in citrus red mite Panonychus citri (McGregor). Phylogenetic analysis indicated that PcETHR was closely related to the insect ETHR subtype B. A calcium mobilization-based functional assay showed that PcETH activated the PcETHR in a dose-dependent manner. Furthermore, a sharp upregulation of PcETH before ecdysis was observed by quantitative real-time PCR (qRT-PCR), and it revealed a tight correlation of ETH signaling and the molting process. These are the first insights into the molecular characterization of ETH and its receptor in mites as P. citri. Our work provides basic information of the ETH and ETHR in P. citri and lays a solid foundation for further physiological functions to better understand the ETH signaling system in mites.
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transcriptome analysis of the citrus red mite Panonychus citri and its gene expression by exposure to insecticide acaricide
Insect Molecular Biology, 2012Co-Authors: J Z Niu, Guy Smagghe, Wei Dou, Guanwang Shen, Kui Zhang, T B Ding, Jinjun WangAbstract:The citrus red mite, Panonychus citri, is known for its ability rapidly to evolve resistance to insecticides/acaricides and to adapt to hosts that produce toxins. In this study, we constructed an unprecedented four gigabase pair transcriptome of P. citri, which was assembled into 64 149 unique transcripts, the functions of which were annotated by five public databases. A total of 116 unique transcripts were identified as representatives of potential involvement in the detoxification of xenobiotics. Genes recorded to encoding insecticide/acaricide target proteins were also obtained from the P. citri transcriptome. In order to explore novel candidate genes potentially involved in the pesticide detoxification of P. citri, we also constructed digital gene expression libraries of short-term transcriptome responses of P. citri to pesticides, which resulted in the identification of 120 unique transcripts potentially associated with insecticide/acaricide detoxification. Our study will facilitate molecular research on pesticide resistance in citrus red mites, as well as in other phytophagous mites.
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Transcriptome analysis of the citrus red mite, Panonychus citri, and its gene expression by exposure to insecticide/acaricide.
Insect molecular biology, 2012Co-Authors: Wei Dou, Guy Smagghe, Guanwang Shen, Kui Zhang, Jinjun WangAbstract:The citrus red mite, Panonychus citri, is known for its ability rapidly to evolve resistance to insecticides/acaricides and to adapt to hosts that produce toxins. In this study, we constructed an unprecedented four gigabase pair transcriptome of P. citri, which was assembled into 64 149 unique transcripts, the functions of which were annotated by five public databases. A total of 116 unique transcripts were identified as representatives of potential involvement in the detoxification of xenobiotics. Genes recorded to encoding insecticide/acaricide target proteins were also obtained from the P. citri transcriptome. In order to explore novel candidate genes potentially involved in the pesticide detoxification of P. citri, we also constructed digital gene expression libraries of short-term transcriptome responses of P. citri to pesticides, which resulted in the identification of 120 unique transcripts potentially associated with insecticide/acaricide detoxification. Our study will facilitate molecular research on pesticide resistance in citrus red mites, as well as in other phytophagous mites.
J.-j. Wang - One of the best experts on this subject based on the ideXlab platform.
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resistance realized heritability and risk assessment of Panonychus citri to avermectin and fenpropathrin
Journal of Applied Ecology, 2011Co-Authors: H G He, Z. M. Zhao, J.-j. WangAbstract:On the basis of resistance breeding and selection in laboratory,and by using the threshold trait analysis in quantitative genetics,this paper studied the realized resistance heritability of Panonychus citri(McGregor) collected from Beibei of Chongqing to avermectin and fenpropathrin,and predicted the resistance risk of P.citri to these two acaricides.After 11-and 16-generations of selection with avermectin and fenpropathrin,the resistance of P.citri to the two acaricides increased by 3.8-and 29.9-fold,and the realized resistance heritability was 0.0475 and 0.1544,respectively.Under laboratory condition,to develop a 10-fold increase of resistance required 12-26 generations of selection for avermectin,and 7-16 generations of selection for fenpropathrin under the selection pressure of 50%-90% mortality for each generation.Under field condition,it would require more generations to develop the same resistance level.Comparing with bioacaricide avermectin,pyrethroid fenpropathrin had obviously higher resistance risk to P.citri.The results provided references for the resistance management of P.citri to acaricides.
