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Matthias Hannig - One of the best experts on this subject based on the ideXlab platform.
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Efficacy of mouthrinses with bovine milk and milk protein isolates to accumulate casein in the in situ Pellicle
Clinical Oral Investigations, 2020Co-Authors: Anna Kensche, Christian Hannig, Sandra Pötschke, A. Dürasch, T. Henle, Matthias HannigAbstract:Objectives The adsorption of bovine milk caseins on the tooth surface might have a positive impact on the prevention of dental diseases. Therefore, the present study aimed to investigate the efficacy of mouthrinses with different types of bovine milk and milk protein isolates to accumulate caseins in the Pellicle. Materials/methods An indirect enzyme-linked immunosorbent assay (ELISA) was established to quantify the amount of caseins adsorbed into the in situ Pellicle. In situ Pellicle samples were collected from 2 volunteers on ceramic specimens (A = 8 cm^2). After 10 min of Pellicle formation, different types of bovine milk, 3% micellar casein in synthetic milk ultrafiltrate (SMUF) or 3% non-micellar caseinate in SMUF, were used as mouthrinses for 10 min. The Pellicle material was harvested after 30 min in situ and examined for caseins by the indirect ELISA. Selected Pellicle samples were subjected to TEM analysis. Results All mouthrinses accumulated caseins in the in situ Pellicle (2.0 ± 0.7–20 ± 1.7 μg/ml) that, under native conditions, expressed no casein signal. Micellar protein association increased the adsorption of casein into the Pellicle. Milk homogenization also had an influence on the casein accumulation in the Pellicle. TEM analysis confirmed the integration of micellar casein into the Pellicle. Conclusion The mouthrinses altered the protein composition and the ultrastructure of the in situ Pellicle to a different extent: bovine milk with 3.8% fat content and 3% micellar casein in SMUF being particularly effective. Clinical relevance The study provides interesting perspectives for innovative prevention strategies in dentistry.
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Efficacy of mouthrinses with bovine milk and milk protein isolates to accumulate casein in the in situ Pellicle.
Clinical oral investigations, 2020Co-Authors: Anna Kensche, Christian Hannig, Sandra Pötschke, A. Dürasch, T. Henle, Matthias HannigAbstract:The adsorption of bovine milk caseins on the tooth surface might have a positive impact on the prevention of dental diseases. Therefore, the present study aimed to investigate the efficacy of mouthrinses with different types of bovine milk and milk protein isolates to accumulate caseins in the Pellicle. An indirect enzyme-linked immunosorbent assay (ELISA) was established to quantify the amount of caseins adsorbed into the in situ Pellicle. In situ Pellicle samples were collected from 2 volunteers on ceramic specimens (A = 8 cm2). After 10 min of Pellicle formation, different types of bovine milk, 3% micellar casein in synthetic milk ultrafiltrate (SMUF) or 3% non-micellar caseinate in SMUF, were used as mouthrinses for 10 min. The Pellicle material was harvested after 30 min in situ and examined for caseins by the indirect ELISA. Selected Pellicle samples were subjected to TEM analysis. All mouthrinses accumulated caseins in the in situ Pellicle (2.0 ± 0.7–20 ± 1.7 μg/ml) that, under native conditions, expressed no casein signal. Micellar protein association increased the adsorption of casein into the Pellicle. Milk homogenization also had an influence on the casein accumulation in the Pellicle. TEM analysis confirmed the integration of micellar casein into the Pellicle. The mouthrinses altered the protein composition and the ultrastructure of the in situ Pellicle to a different extent: bovine milk with 3.8% fat content and 3% micellar casein in SMUF being particularly effective. The study provides interesting perspectives for innovative prevention strategies in dentistry.
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impact of oral astringent stimuli on surface charge and morphology of the protein rich Pellicle at the tooth saliva interphase
Colloids and Surfaces B: Biointerfaces, 2019Co-Authors: Ralf Zimmermann, Judith Delius, Thomas Hofmann, Melanie Rehage, Jens Friedrichs, Susanne Stehl, Christian Hannig, Carsten Werner, Matthias HannigAbstract:Abstract The proteinaceous Pellicle layer, which develops upon contact with saliva on the surface of teeth, is important for the formation of oral biofilms and for the protection of teeth from abrasion and chemically induced erosion. Astringent food ingredients comprising polyphenols, cationic macromolecules, and multivalent metal salts are known to interact with the Pellicle. However, astringent-induced changes in the physicochemical properties of the tooth-saliva interphase are not yet completely understood. Here we provide comprehensive insights into interfacial charging, ultrastructure, thickness, and surface roughness of the Pellicles formed on the model substrates silicon oxide (SiO2), Teflon® AF, and hydroxyapatite, as well as on bovine enamel before and after incubation with the astringents epigallocatechin gallate, tannic acid, iron(III) salt, lysozyme, and chitosan. Quartz crystal microbalance with dissipation monitoring demonstrated viscous behavior of untreated Pellicles formed in vitro on the different materials. Electrokinetic (streaming current) measurements revealed that cationic astringents reverse the charge of native Pellicles, whereas polyphenols did not change the charge under physiological pH condition. In addition, transmission electron microscopy and atomic force microscopy showed a concentration-dependent increase in average film thickness and Pellicle surface roughness as induced by astringents. These multifaceted alterations of the salivary Pellicle may come along with an increase in roughness perceived on the teeth, which is part of the complex sensations of oral astringency.
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oral astringent stimuli alter the enamel Pellicle s ultrastructure as revealed by electron microscopy
Journal of Dentistry, 2017Co-Authors: Melanie Rehage, Judith Delius, Thomas Hofmann, Matthias HannigAbstract:Abstract Objectives This electron microscopic study aimed at investigating effects of oral astringent stimuli on the enamel Pellicle’s morphology. Methods Pellicles were formed in situ within 30 min on bovine enamel slabs, fixed to individuals’ upper jaw splints. The Pellicle-coated specimens were immersed in vitro in seven diverse astringent solutions and subsequently analyzed by scanning electron microscopy (SEM), energy dispersive X-ray (EDX) spectroscopy, as well as transmission electron microscopy (TEM). Four biocompatible astringents, namely the polyphenol epigallocatechin gallate, the metal salt iron(III) sulfate, the basic protein lysozyme, and the aminopolysaccharide chitosan, were additionally applied in situ. After rinsing the oral cavity with these compounds, the Pellicle’s ultrastructure was imaged by SEM and TEM, respectively. Untreated Pellicle samples served as controls. Results Exposure to polyphenols and lysozyme induced particularly thicker and electron-denser Pellicles in comparison to the control Pellicle with similar characteristics in vitro and in situ. In contrast, acidic chitosan and metal salt solutions, respectively, revealed minor Pellicle alterations. The incorporation of Fe and Al into the Pellicles treated with the corresponding inorganic salts was verified by EDX analysis. Conclusions Astringent-induced Pellicle modifications were for the first time visualized by TEM. The ultrastructural alterations of the dental Pellicle may partly explain the tooth-roughening effect caused by oral astringent stimuli. Clinical significance Astringents might modify the Pellicle’s protective properties against dental erosion, attrition, as well as bacterial adhesion, and by this means may influence tooth health. The findings may thus be particularly relevant for preventive dentistry.
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Effect of Inula viscosa on the Pellicle’s protective properties and initial bioadhesion in-situ
Archives of oral biology, 2016Co-Authors: Susann Hertel, Matthias Hannig, Wiebke Hoth-hannig, Sandra Pötschke, Leif Graffy, Sabine Basche, Ali Al-ahmad, Christian HannigAbstract:The present in situ study investigated the effect of Inula viscosa tea on the Pellicle's acid protective properties and on initial oral biofilm formation. Biofilm formation was performed on bovine enamel slabs on individual maxillary splints. Following 1min of Pellicle formation, eight subjects rinsed for 10min with Inula viscosa tea and the splints remained for 8h intraorally. Samples carried after 1-min rinsing with CHX (0.2%) or without rinse served as controls. BacLight™ staining, 4',6-diamidino-2-phenylindole (DAPI)-staining and fluorescence in situ hybridization (FISH) were used for fluorescence microscopic detection of adherent bacteria. For investigation of acid protective properties, three subjects rinsed for 10min with Inula viscosa tea after 1min Pellicle formation and kept the splints intraorally for further 19min. Physiological 30-min Pellicles and native enamel samples served as controls. After HCl incubation of the samples ex-vivo over 120s (pH 2.0, 2.3, 3.0) calcium- and phosphate release were quantified photometrically. Potential influences on the Pellicle's ultrastructure by Inula viscosa tea were evaluated by transmission electron microscopy (TEM). Application of Inula viscosa tea yielded a significant reduction of adherent bacteria on all enamel samples as detected by fluorescence microscopy. For calcium- and phosphate release no significant effect was recorded. TEM investigation indicated a modification of the Pellicle's ultrastructure, but no enhanced protection against erosive noxae. Rinsing with Inula viscosa tea influences the bacterial colonization on enamel in situ over 8h but has no impact on acid protective properties of the Pellicle. Copyright © 2016 Elsevier Ltd. All rights reserved.
Christian Hannig - One of the best experts on this subject based on the ideXlab platform.
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Efficacy of mouthrinses with bovine milk and milk protein isolates to accumulate casein in the in situ Pellicle
Clinical Oral Investigations, 2020Co-Authors: Anna Kensche, Christian Hannig, Sandra Pötschke, A. Dürasch, T. Henle, Matthias HannigAbstract:Objectives The adsorption of bovine milk caseins on the tooth surface might have a positive impact on the prevention of dental diseases. Therefore, the present study aimed to investigate the efficacy of mouthrinses with different types of bovine milk and milk protein isolates to accumulate caseins in the Pellicle. Materials/methods An indirect enzyme-linked immunosorbent assay (ELISA) was established to quantify the amount of caseins adsorbed into the in situ Pellicle. In situ Pellicle samples were collected from 2 volunteers on ceramic specimens (A = 8 cm^2). After 10 min of Pellicle formation, different types of bovine milk, 3% micellar casein in synthetic milk ultrafiltrate (SMUF) or 3% non-micellar caseinate in SMUF, were used as mouthrinses for 10 min. The Pellicle material was harvested after 30 min in situ and examined for caseins by the indirect ELISA. Selected Pellicle samples were subjected to TEM analysis. Results All mouthrinses accumulated caseins in the in situ Pellicle (2.0 ± 0.7–20 ± 1.7 μg/ml) that, under native conditions, expressed no casein signal. Micellar protein association increased the adsorption of casein into the Pellicle. Milk homogenization also had an influence on the casein accumulation in the Pellicle. TEM analysis confirmed the integration of micellar casein into the Pellicle. Conclusion The mouthrinses altered the protein composition and the ultrastructure of the in situ Pellicle to a different extent: bovine milk with 3.8% fat content and 3% micellar casein in SMUF being particularly effective. Clinical relevance The study provides interesting perspectives for innovative prevention strategies in dentistry.
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Efficacy of mouthrinses with bovine milk and milk protein isolates to accumulate casein in the in situ Pellicle.
Clinical oral investigations, 2020Co-Authors: Anna Kensche, Christian Hannig, Sandra Pötschke, A. Dürasch, T. Henle, Matthias HannigAbstract:The adsorption of bovine milk caseins on the tooth surface might have a positive impact on the prevention of dental diseases. Therefore, the present study aimed to investigate the efficacy of mouthrinses with different types of bovine milk and milk protein isolates to accumulate caseins in the Pellicle. An indirect enzyme-linked immunosorbent assay (ELISA) was established to quantify the amount of caseins adsorbed into the in situ Pellicle. In situ Pellicle samples were collected from 2 volunteers on ceramic specimens (A = 8 cm2). After 10 min of Pellicle formation, different types of bovine milk, 3% micellar casein in synthetic milk ultrafiltrate (SMUF) or 3% non-micellar caseinate in SMUF, were used as mouthrinses for 10 min. The Pellicle material was harvested after 30 min in situ and examined for caseins by the indirect ELISA. Selected Pellicle samples were subjected to TEM analysis. All mouthrinses accumulated caseins in the in situ Pellicle (2.0 ± 0.7–20 ± 1.7 μg/ml) that, under native conditions, expressed no casein signal. Micellar protein association increased the adsorption of casein into the Pellicle. Milk homogenization also had an influence on the casein accumulation in the Pellicle. TEM analysis confirmed the integration of micellar casein into the Pellicle. The mouthrinses altered the protein composition and the ultrastructure of the in situ Pellicle to a different extent: bovine milk with 3.8% fat content and 3% micellar casein in SMUF being particularly effective. The study provides interesting perspectives for innovative prevention strategies in dentistry.
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impact of oral astringent stimuli on surface charge and morphology of the protein rich Pellicle at the tooth saliva interphase
Colloids and Surfaces B: Biointerfaces, 2019Co-Authors: Ralf Zimmermann, Judith Delius, Thomas Hofmann, Melanie Rehage, Jens Friedrichs, Susanne Stehl, Christian Hannig, Carsten Werner, Matthias HannigAbstract:Abstract The proteinaceous Pellicle layer, which develops upon contact with saliva on the surface of teeth, is important for the formation of oral biofilms and for the protection of teeth from abrasion and chemically induced erosion. Astringent food ingredients comprising polyphenols, cationic macromolecules, and multivalent metal salts are known to interact with the Pellicle. However, astringent-induced changes in the physicochemical properties of the tooth-saliva interphase are not yet completely understood. Here we provide comprehensive insights into interfacial charging, ultrastructure, thickness, and surface roughness of the Pellicles formed on the model substrates silicon oxide (SiO2), Teflon® AF, and hydroxyapatite, as well as on bovine enamel before and after incubation with the astringents epigallocatechin gallate, tannic acid, iron(III) salt, lysozyme, and chitosan. Quartz crystal microbalance with dissipation monitoring demonstrated viscous behavior of untreated Pellicles formed in vitro on the different materials. Electrokinetic (streaming current) measurements revealed that cationic astringents reverse the charge of native Pellicles, whereas polyphenols did not change the charge under physiological pH condition. In addition, transmission electron microscopy and atomic force microscopy showed a concentration-dependent increase in average film thickness and Pellicle surface roughness as induced by astringents. These multifaceted alterations of the salivary Pellicle may come along with an increase in roughness perceived on the teeth, which is part of the complex sensations of oral astringency.
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Effect of Inula viscosa on the Pellicle’s protective properties and initial bioadhesion in-situ
Archives of oral biology, 2016Co-Authors: Susann Hertel, Matthias Hannig, Wiebke Hoth-hannig, Sandra Pötschke, Leif Graffy, Sabine Basche, Ali Al-ahmad, Christian HannigAbstract:The present in situ study investigated the effect of Inula viscosa tea on the Pellicle's acid protective properties and on initial oral biofilm formation. Biofilm formation was performed on bovine enamel slabs on individual maxillary splints. Following 1min of Pellicle formation, eight subjects rinsed for 10min with Inula viscosa tea and the splints remained for 8h intraorally. Samples carried after 1-min rinsing with CHX (0.2%) or without rinse served as controls. BacLight™ staining, 4',6-diamidino-2-phenylindole (DAPI)-staining and fluorescence in situ hybridization (FISH) were used for fluorescence microscopic detection of adherent bacteria. For investigation of acid protective properties, three subjects rinsed for 10min with Inula viscosa tea after 1min Pellicle formation and kept the splints intraorally for further 19min. Physiological 30-min Pellicles and native enamel samples served as controls. After HCl incubation of the samples ex-vivo over 120s (pH 2.0, 2.3, 3.0) calcium- and phosphate release were quantified photometrically. Potential influences on the Pellicle's ultrastructure by Inula viscosa tea were evaluated by transmission electron microscopy (TEM). Application of Inula viscosa tea yielded a significant reduction of adherent bacteria on all enamel samples as detected by fluorescence microscopy. For calcium- and phosphate release no significant effect was recorded. TEM investigation indicated a modification of the Pellicle's ultrastructure, but no enhanced protection against erosive noxae. Rinsing with Inula viscosa tea influences the bacterial colonization on enamel in situ over 8h but has no impact on acid protective properties of the Pellicle. Copyright © 2016 Elsevier Ltd. All rights reserved.
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The oral cavity—a key system to understand substratum-dependent bioadhesion on solid surfaces in man
Clinical Oral Investigations, 2009Co-Authors: Christian Hannig, Matthias HannigAbstract:One of the greatest challenges in life sciences and biomaterials research is adhesion of biomolecules and bacteria to solid surfaces in aqueous solutions. An example concerning everybody is biofilm formation in the oral cavity on dental materials and dental hard substances, respectively. The main characteristics typical for any bioadhesion can be observed excellently in the oral cavity. Initially, a proteinaceous layer termed Pellicle is formed. It mediates the interactions between solid substrata, oral fluids and microorganisms. Numerous different materials with differing physico-chemical properties and possible impact on the acquired Pellicle are present in the oral cavity such as enamel, dentine, restorative materials or dental implants. Despite the fact that in vitro studies demonstrate considerable differences of experimental Pellicles formed on these materials, the in situ Pellicles seem to be relatively similar and level off the different properties of the underlying substrates. However, the bacterial colonisation of Pellicle-coated surfaces under in vivo conditions differs considerably. Long-range forces and detachment of biofilm layers may account for this phenomenon despite the masking effect of the Pellicle. Accordingly, low-energy surfaces are desirable for restorative materials exposed to the oral cavity to minimise bacterial adhesion. The oral cavity is an easy accessible in vivo model for understanding bioadhesion and for investigation of protein–surface interactions noninvasively. For evaluation of biofilm formation on dental materials, in situ or in vivo studies are preferable.
Sima Yaron - One of the best experts on this subject based on the ideXlab platform.
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Morphological analysis of young and old Pellicles of Salmonella Typhimurium.
Biofouling, 2007Co-Authors: Keren Scher, Ellina Kesselman, Eyal Shimoni, Sima YaronAbstract:A wide variety of microorganisms are able to form biofilms at the interface between air and liquid (Pellicles). In this study changes during the maturation of the Pellicle of Salmonella Typhimurium were analysed and the role of cellulose in the Pellicle structure and morphology evaluated. The morphology of both sides of the Pellicle was characterised using atomic force microscopy and scanning electron microscopy. Overall, there was a marked difference in the morphology of the water-facing (WF) and air-facing (AF) biofilm surfaces. While the AF side appeared to be uniform, and extensively covered with an exocellular coating, cells in the WF side were distributed into clusters and were less covered. However, the similarity in size and shape of single cells from both sides of the Pellicle may indicate that the bacterial cells across the Pellicle have a similar physiological status. During maturation, porous structures with multiple cracks and channels were created in the Pellicle, leading to disintegration. By comparison with the structure of Pellicles of a cellulose-deficient mutant, it was demonstrated that the observed disintegration of mature Pellicles probably occurred in part by self-hydrolysis of components of the matrix.
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Morphological analysis of young and old Pellicles of Salmonella Typhimurium.
Biofouling, 2007Co-Authors: Keren Scher, Ellina Kesselman, Eyal Shimoni, Sima YaronAbstract:Abstract A wide variety of microorganisms are able to form biofilms at the interface between air and liquid (Pellicles). In this study changes during the maturation of the Pellicle of Salmonella Typhimurium were analysed and the role of cellulose in the Pellicle structure and morphology evaluated. The morphology of both sides of the Pellicle was characterised using atomic force microscopy and scanning electron microscopy. Overall, there was a marked difference in the morphology of the water-facing (WF) and air-facing (AF) biofilm surfaces. While the AF side appeared to be uniform, and extensively covered with an exocellular coating, cells in the WF side were distributed into clusters and were less covered. However, the similarity in size and shape of single cells from both sides of the Pellicle may indicate that the bacterial cells across the Pellicle have a similar physiological status. During maturation, porous structures with multiple cracks and channels were created in the Pellicle, leading to disinte...
Keren Scher - One of the best experts on this subject based on the ideXlab platform.
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Morphological analysis of young and old Pellicles of Salmonella Typhimurium.
Biofouling, 2007Co-Authors: Keren Scher, Ellina Kesselman, Eyal Shimoni, Sima YaronAbstract:A wide variety of microorganisms are able to form biofilms at the interface between air and liquid (Pellicles). In this study changes during the maturation of the Pellicle of Salmonella Typhimurium were analysed and the role of cellulose in the Pellicle structure and morphology evaluated. The morphology of both sides of the Pellicle was characterised using atomic force microscopy and scanning electron microscopy. Overall, there was a marked difference in the morphology of the water-facing (WF) and air-facing (AF) biofilm surfaces. While the AF side appeared to be uniform, and extensively covered with an exocellular coating, cells in the WF side were distributed into clusters and were less covered. However, the similarity in size and shape of single cells from both sides of the Pellicle may indicate that the bacterial cells across the Pellicle have a similar physiological status. During maturation, porous structures with multiple cracks and channels were created in the Pellicle, leading to disintegration. By comparison with the structure of Pellicles of a cellulose-deficient mutant, it was demonstrated that the observed disintegration of mature Pellicles probably occurred in part by self-hydrolysis of components of the matrix.
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Morphological analysis of young and old Pellicles of Salmonella Typhimurium.
Biofouling, 2007Co-Authors: Keren Scher, Ellina Kesselman, Eyal Shimoni, Sima YaronAbstract:Abstract A wide variety of microorganisms are able to form biofilms at the interface between air and liquid (Pellicles). In this study changes during the maturation of the Pellicle of Salmonella Typhimurium were analysed and the role of cellulose in the Pellicle structure and morphology evaluated. The morphology of both sides of the Pellicle was characterised using atomic force microscopy and scanning electron microscopy. Overall, there was a marked difference in the morphology of the water-facing (WF) and air-facing (AF) biofilm surfaces. While the AF side appeared to be uniform, and extensively covered with an exocellular coating, cells in the WF side were distributed into clusters and were less covered. However, the similarity in size and shape of single cells from both sides of the Pellicle may indicate that the bacterial cells across the Pellicle have a similar physiological status. During maturation, porous structures with multiple cracks and channels were created in the Pellicle, leading to disinte...
Jizhong Zhou - One of the best experts on this subject based on the ideXlab platform.
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Transcriptome analysis of Pellicle formation of Shewanella oneidensis
Archives of microbiology, 2012Co-Authors: Yili Liang, Haichun Gao, Xue Guo, Jingrong Chen, Guanzhou Qiu, Jizhong Zhou, Xueduan LiuAbstract:Although the Pellicle is one of the major growth modes of microorganisms, the metabolic features of Pellicle cells and the determinative factors for Pellicle formation are largely unknown. In recent years, biofilm development of Shewanella oneidensis, an important model organism for bioremediation studies, has been extensively studied. In this paper, a transcriptional profiling of Pellicle cells relative to planktonic cells indicated that cells in Pellicles were more metabolically active than the planktonic cells. Most notably, up-transcription of general secretion system proteins and iron/heme uptake and transport proteins was observed in Pellicle cells. Unexpectedly, neither the hmuT nor hugA heme transport mutant exhibited a significant defect in Pellicle formation. Expectedly, three type I secretion system mutants were severely deficient in Pellicle formation, suggesting an essential role of these proteins.
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Pellicle formation in Shewanella oneidensis
BMC microbiology, 2010Co-Authors: Yili Liang, Haichun Gao, Jingrong Chen, Guanzhou Qiu, Xueduan Liu, Yangyang Dong, Jizhong ZhouAbstract:Background Although solid surface-associated biofilm development of S. oneidensis has been extensively studied in recent years, Pellicles formed at the air-liquid interface are largely overlooked. The goal of this work was to understand basic requirements and mechanism of Pellicle formation in S. oneidensis.