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Cedryck Vaquette - One of the best experts on this subject based on the ideXlab platform.
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tissue engineered constructs for Periodontal Regeneration current status and future perspectives
Advanced Healthcare Materials, 2018Co-Authors: William V Giannobile, Mark P Bartold, Cedryck Vaquette, Sophia P Pilipchuk, Dietmar W Hutmacher, Saso IvanovskiAbstract:The periodontium, consisting of gingiva, Periodontal ligament, cementum, and alveolar bone, is a hierarchically organized tissue whose primary role is to provide physical and mechanical support to the teeth. Severe cases of periodontitis, an inflammatory condition initiated by an oral bacterial biofilm, can lead to significant destruction of soft and hard tissues of the periodontium and result in compromised dental function and aesthetics. Although current treatment approaches can limit the progression of the disease by controlling the inflammatory aspect, complete Periodontal Regeneration cannot be predictably achieved. Various tissue engineering approaches are investigated for their ability to control the critical temporo-spatial wound healing events that are essential for achieving Periodontal Regeneration. This paper reviews recent progress in the field of Periodontal tissue engineering with an emphasis on advanced 3D multiphasic tissue engineering constructs (TECs) and provides a critical analysis of their regenerative potential and limitations. The review also elaborates on the future of Periodontal tissue engineering, including scaffold customization for individual Periodontal defects, TEC's functionalization strategies for imparting enhanced bioactivity, Periodontal ligament fiber guidance, and the utilization of chair-side regenerative solutions that can facilitate clinical translation.
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the influence of cellular source on Periodontal Regeneration using calcium phosphate coated polycaprolactone scaffold supported cell sheets
Biomaterials, 2014Co-Authors: Cedryck Vaquette, Yin Xiao, Dietmar W Hutmacher, Anthony Fisher, Stephen Hamlet, Saso IvanovskiAbstract:Cell-based therapy is considered a promising approach to achieving predictable Periodontal Regeneration. In this study, the regenerative potential of cell sheets derived from different parts of the periodontium (gingival connective tissue, alveolar bone and Periodontal ligament) were investigated in an athymic rat Periodontal defect model. Periodontal ligament (PDLC), alveolar bone (ABC) and gingival margin-derived cells (GMC) were obtained from human donors. The osteogenic potential of the primary cultures was demonstrated in vitro. Cell sheets supported by a calcium phosphate coated melt electrospun polycaprolactone (CaP-PCL) scaffold were transplanted to denuded root surfaces in surgically created Periodontal defects, and allowed to heal for 1 and 4 weeks. The CaP-PCL scaffold alone was able to promote alveolar bone formation within the defect after 4 weeks. The addition of ABC and PDLC sheets resulted in significant Periodontal attachment formation. The GMC sheets did not promote Periodontal Regeneration on the root surface and inhibited bone formation within the CaP-PCL scaffold. In conclusion, the combination of either PDLC or ABC sheets with a CaP-PCL scaffold could promote Periodontal Regeneration, but ABC sheets were not as effective as PDLC sheets in promoting new attachment formation.
Saso Ivanovski - One of the best experts on this subject based on the ideXlab platform.
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tissue engineered constructs for Periodontal Regeneration current status and future perspectives
Advanced Healthcare Materials, 2018Co-Authors: William V Giannobile, Mark P Bartold, Cedryck Vaquette, Sophia P Pilipchuk, Dietmar W Hutmacher, Saso IvanovskiAbstract:The periodontium, consisting of gingiva, Periodontal ligament, cementum, and alveolar bone, is a hierarchically organized tissue whose primary role is to provide physical and mechanical support to the teeth. Severe cases of periodontitis, an inflammatory condition initiated by an oral bacterial biofilm, can lead to significant destruction of soft and hard tissues of the periodontium and result in compromised dental function and aesthetics. Although current treatment approaches can limit the progression of the disease by controlling the inflammatory aspect, complete Periodontal Regeneration cannot be predictably achieved. Various tissue engineering approaches are investigated for their ability to control the critical temporo-spatial wound healing events that are essential for achieving Periodontal Regeneration. This paper reviews recent progress in the field of Periodontal tissue engineering with an emphasis on advanced 3D multiphasic tissue engineering constructs (TECs) and provides a critical analysis of their regenerative potential and limitations. The review also elaborates on the future of Periodontal tissue engineering, including scaffold customization for individual Periodontal defects, TEC's functionalization strategies for imparting enhanced bioactivity, Periodontal ligament fiber guidance, and the utilization of chair-side regenerative solutions that can facilitate clinical translation.
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the influence of cellular source on Periodontal Regeneration using calcium phosphate coated polycaprolactone scaffold supported cell sheets
Biomaterials, 2014Co-Authors: Cedryck Vaquette, Yin Xiao, Dietmar W Hutmacher, Anthony Fisher, Stephen Hamlet, Saso IvanovskiAbstract:Cell-based therapy is considered a promising approach to achieving predictable Periodontal Regeneration. In this study, the regenerative potential of cell sheets derived from different parts of the periodontium (gingival connective tissue, alveolar bone and Periodontal ligament) were investigated in an athymic rat Periodontal defect model. Periodontal ligament (PDLC), alveolar bone (ABC) and gingival margin-derived cells (GMC) were obtained from human donors. The osteogenic potential of the primary cultures was demonstrated in vitro. Cell sheets supported by a calcium phosphate coated melt electrospun polycaprolactone (CaP-PCL) scaffold were transplanted to denuded root surfaces in surgically created Periodontal defects, and allowed to heal for 1 and 4 weeks. The CaP-PCL scaffold alone was able to promote alveolar bone formation within the defect after 4 weeks. The addition of ABC and PDLC sheets resulted in significant Periodontal attachment formation. The GMC sheets did not promote Periodontal Regeneration on the root surface and inhibited bone formation within the CaP-PCL scaffold. In conclusion, the combination of either PDLC or ABC sheets with a CaP-PCL scaffold could promote Periodontal Regeneration, but ABC sheets were not as effective as PDLC sheets in promoting new attachment formation.
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Gene Expression Profiling of Cells Involved in Periodontal Regeneration
2007Co-Authors: Saso Ivanovski, Agnieszka M. Lichanska, Elisabetta M. Aniello, Yin XiaoAbstract:Understanding the molecular mechanisms involved in Periodontal Regeneration is important for the development of more predictable clinical techniques. This study aimed to identify these mechanisms by comparing the gene expression profiles of cells derived from regenerating defects with patient-matched Periodontal ligament cells. The gene profiling was carried out using Affymetrix U133A arrays containing probes for 22000 genes. Robust differences in gene expression were obtained by identifying genes that consistently changed by a minimum of two fold. Analysis of molecular function as designated by gene ontology (GO) identified differentially regulated mechanisms including protein metabolism, tyrosine kinase activity and skeletal development. The differentially expressed genes could be broadly divided into the categories of protein biosynthesis and turnover, structural constituents of the cytoskeleton and extracellular matrix, and signal transduction. The differential expression of four genes (EGR-1, elastin, osteoprotegerin and IGFBP3) was confirmed using real time PCR. Furthermore, the expression of another two differentially expressed transcripts, decorin and biglycan, was immunohistochemically confirmed in a Periodontal wound healing model and the protein expression was consistent with the pattern of gene expression. This study gives insight into the molecular processes involved in Periodontal Regeneration and identifies cell markers that are characteristic of the regenerating Periodontal tissues.
Mark A Reynolds - One of the best experts on this subject based on the ideXlab platform.
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Protein and peptide-based therapeutics in Periodontal Regeneration.
The journal of evidence-based dental practice, 2020Co-Authors: Mark A Reynolds, Mary E Aichelmann-reidyAbstract:Protein and peptide-based therapeutics provide a unique strategy for controlling highly specific and complex biologic actions that cannot be accomplished by simple devices or chemical compounds. This article reviews some of the key characteristics and summarizes the clinical effectiveness of protein and peptide-based therapeutics targeting Periodontal Regeneration. A literature search was conducted of randomized clinical trials and systematic reviews evaluating protein and peptide-based therapeutics for the Regeneration of Periodontal tissues of at least 6 months duration. Data sources included PubMed and Embase electronic databases, hand-searched journals, and the ClinicalTrials.gov registry. Commercially marketed protein and peptide-based therapeutics for Periodontal Regeneration provide gains in clinical attachment level and bone formation that are comparable or superior to other regenerative approaches. Results from several clinical trials indicate that protein and peptide-based therapies can accelerate repair and Regeneration when compared with other treatments and that improvements in clinical parameters continue beyond 12 months. Protein and peptide-based therapies also exhibit the capacity to increase the predictability of treatment outcomes. Clinical and histologic studies support the effectiveness of protein- and peptide-based therapeutics for Periodontal Regeneration. Emerging evidence suggests that the delivery devices/scaffolds play a critical role in determining the effectiveness of this class of therapeutics. Copyright © 2012 Elsevier Inc. All rights reserved.
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Periodontal Regeneration intrabony defects a systematic review from the aap Regeneration workshop
Journal of Periodontology, 2015Co-Authors: Salvador Nares, Mark A ReynoldsAbstract:Background: Previous systematic reviews of Periodontal Regeneration with bone replacement grafts and guided tissue Regeneration (GTR) were defined as state of the art for clinical Periodontal Regeneration as of 2002.Methods: The purpose of this systematic review is to update those consensus reports by reviewing Periodontal Regeneration approaches developed for the correction of intrabony defects with the focus on patient-, tooth-, and site-centered factors, surgical approaches, surgical determinants, and biologics. This review adheres to the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines for systematic reviews.A computerized search of the PubMed and Cochrane databases was performed to evaluate the clinically available regenerative approaches for intrabony defects. The search included screening of original reports, review articles, and reference lists of retrieved articles and hand searches of selected journals.All searches were focused on clinically available regene...
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Periodontal Regeneration intrabony defects a consensus report from the aap Regeneration workshop
Journal of Periodontology, 2015Co-Authors: Mark A Reynolds, Jack G. Caton, Salvador Nares, Paulo M Camargo, Donald S Clem, Joseph P Fiorellini, Maria L Geisinger, Michael P Mills, Marc L NevinsAbstract:Background: Treatment of intrabony defects is an important therapeutic goal of Periodontal therapy. The goal of this consensus report was to critically appraise the evidence for the available approaches for promoting Periodontal Regeneration in intrabony defects. In addition to evaluating the effectiveness of new regenerative approaches for intrabony defects, recommendations for future research were defined for this area.Methods: A systematic review was conducted using computerized searches of PubMed and Cochrane databases, supplemented with screening of references in original reports, review articles, and a hand search in selected journals. All searches were focused on regenerative approaches with histologic evidence of Periodontal Regeneration (proof of principle), clinical trials, and case reports. For purposes of analysis, change in intrabony defect fill was considered the primary outcome variable, with change in clinical attachment as a secondary outcome. The SORT (Strength of Recommendation Taxonomy...
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Periodontal Regeneration – Intrabony Defects: A Systematic Review From the AAP Regeneration Workshop
Journal of Periodontology, 2014Co-Authors: Salvador Nares, Mark A ReynoldsAbstract:Background: Previous systematic reviews of Periodontal Regeneration with bone replacement grafts and guided tissue Regeneration (GTR) were defined as state of the art for clinical Periodontal Regeneration as of 2002.Methods: The purpose of this systematic review is to update those consensus reports by reviewing Periodontal Regeneration approaches developed for the correction of intrabony defects with the focus on patient-, tooth-, and site-centered factors, surgical approaches, surgical determinants, and biologics. This review adheres to the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines for systematic reviews.A computerized search of the PubMed and Cochrane databases was performed to evaluate the clinically available regenerative approaches for intrabony defects. The search included screening of original reports, review articles, and reference lists of retrieved articles and hand searches of selected journals.All searches were focused on clinically available regene...
Stan Gronthos - One of the best experts on this subject based on the ideXlab platform.
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Periodontal ligament derived cells for Periodontal Regeneration in animal models a systematic review
Journal of Periodontal Research, 2015Co-Authors: Richard Bright, Stan Gronthos, Kim Hynes, P. M. BartoldAbstract:Background and objective Implantation of Periodontal ligament stem cells is emerging as a potential Periodontal regenerative procedure. This systematic review considers the evidence from animal models investigating the use of Periodontal ligament stem cells for successful Periodontal Regeneration. Material and methods PubMed, Embase, MEDLINE and Google Scholar were searched to December 2013 for quantitative studies examining the outcome of implanting Periodontal ligament stem cells into experimental Periodontal defects in animals. Inclusion criteria were: implantation of Periodontal ligament stem cells into surgically created Periodontal defects for Periodontal Regeneration; animal models only; source of cells either human or animal; and published in English. We followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Results From the literature search, 43 studies met the inclusion criteria. A wide variety of surgical defects were created in four species of animal (dog, rat, pig and sheep). Owing to wide variability in defect type, cell source and cell scaffold, no meta-analysis was possible. Outcome measures included new bone, new cementum and new connective tissue formation. In 70.5% of the results, statistically significant improvements of these measures was recorded. Conclusion These results are notable in that they indicate that irrespective of the defect type and animal model used, Periodontal ligament stem cell implantation can be expected to result in a beneficial outcome for Periodontal Regeneration. It is recommended that there is sufficient evidence from preclinical animal studies to warrant moving to human studies to examine the efficacy, safety, feasibility (autologous vs. allogeneic transplantation) and delivery of Periodontal ligament stem cells for Periodontal Regeneration.
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stem cells tissue engineering and Periodontal Regeneration
Australian Dental Journal, 2014Co-Authors: Danijela Menicanin, Stan Gronthos, P Mark BartoldAbstract:: The aim of this review is to discuss the clinical utility of stem cells in Periodontal Regeneration by reviewing relevant literature that assesses the Periodontal-regenerative potential of stem cells. We consider and describe the main stem cell populations that have been utilized with regard to Periodontal Regeneration, including bone marrow-derived mesenchymal stem cells and the main dental-derived mesenchymal stem cell populations: Periodontal ligament stem cells, dental pulp stem cells, stem cells from human exfoliated deciduous teeth, stem cells from apical papilla and dental follicle precursor cells. Research into the use of stem cells for tissue Regeneration has the potential to significantly influence Periodontal treatment strategies in the future.
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clinical utility of stem cells for Periodontal Regeneration
Periodontology 2000, 2012Co-Authors: Kim Hynes, Stan Gronthos, Danijela Menicanin, Mark P BartoldAbstract:Abstract The aim of this review is to discuss the clinical utility of stem cells in Periodontal Regeneration by reviewing relevant literature that assesses the Periodontal-regenerative potential of stem cells. We considered and described the main stem cell populations that have been utilized with regard to Periodontal Regeneration, including bone marrow-derived mesenchymal stem cells and the main dental-derived mesenchymal stem cell populations: Periodontal ligament stem cells, dental pulp stem cells, stem cells from human exfoliated deciduous teeth, stem cells from apical papilla and dental follicle precursor cells. Research into the use of stem cells for tissue Regeneration has the potential to significantly influence Periodontal treatment strategies in the future.
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stem cells and Periodontal Regeneration
Australian Dental Journal, 2008Co-Authors: Stan Gronthos, P Mark BartoldAbstract:Periodontitis is an inflammatory disease which manifests clinically as loss of supporting Periodontal tissues including Periodontal ligament and alveolar bone. For decades periodontists have sought ways to repair the damage which occurs during periodontitis. This has included the use of a range of surgical procedures, the use of a variety of grafting materials and growth factors, and the use of barrier membranes. To date Periodontal Regeneration is considered to be biologically possible but clinically unpredictable. Recently, reports have begun to emerge demonstrating that populations of adult stem cells reside in the Periodontal ligament of humans and other animals. This opens the way for new cell-based therapies for Periodontal Regeneration. For this to become a reality a thorough understanding of adult human stem cells is needed. This review provides an overview of adult human stem cells and their potential use in Periodontal Regeneration.
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Stem cells and Periodontal Regeneration
Australian Dental Journal, 2008Co-Authors: N-h Lin, Stan Gronthos, P Mark BartoldAbstract:Periodontitis is an inflammatory disease which manifests clinically as loss of supporting Periodontal tissues including Periodontal ligament and alveolar bone. For decades periodontists have sought ways to repair the damage which occurs during periodontitis. This has included the use of a range of surgical procedures, the use of a variety of grafting materials and growth factors, and the use of barrier membranes. To date Periodontal Regeneration is considered to be biologically possible but clinically unpredictable. Recently, reports have begun to emerge demonstrating that populations of adult stem cells reside in the Periodontal ligament of humans and other animals. This opens the way for new cell-based therapies for Periodontal Regeneration. For this to become a reality a thorough understanding of adult human stem cells is needed. This review provides an overview of adult human stem cells and their potential use in Periodontal Regeneration. Abbreviations and acronyms: BMP = bone morphogenetic proteins; BMP-7 = bone morphogenetic protein-7; BMSSCs = bone marrow stromal stem cells; EGF = epidermal growth factor; ePTFE = expanded polytetrafluoroethylene; ES = embryonic stem; FGF = fibroblast growth factor; IGF = insulin-like growth factor; HA ⁄ TCP = hydroxyapatite ⁄ tricalcium phosphate ceramic; MHC = major histocompat-ibility; MSCs = mesenchymal stem cells; PDGF = platelet-derived growth factor; PDLSCs = Periodontal ligament stem cells.
John E Davies - One of the best experts on this subject based on the ideXlab platform.
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Periodontal Regeneration using a bilayered plga calcium phosphate construct
Biomaterials, 2011Co-Authors: Emily Correna Carlo Reis, Andrea Pacheco Batista Borges, Michel Furtado V Araujo, Vanessa C Mendes, Limin Guan, John E DaviesAbstract:The Regeneration of tissues affected by Periodontal disease is a complex process; it encompasses the formation of bone, cementum and Periodontal ligament. We developed a semi-rigid PLGA (polylactide-co-glycolide acid)/CaP (calcium phosphate) bilayered biomaterial construct to promote Periodontal Regeneration, which has a continuous outer barrier membrane and an inner topographically complex component. Our experimental model compared Periodontal prophylaxis alone with prophylaxis and biomaterial implantation in the treatment of class II furcation defects in dogs. Clinical evaluation, micro-computed tomography, histology and backscattered electron imaging were used for data analysis. Healing occurred uneventfully and bone volumetric values, trabecular number and trabecular thickness were all significantly greater in the treated group; while trabecular separation was significantly greater in the control group. New cementum, bone, and Periodontal ligament with Sharpey fibre insertions were only seen in the treated group. Although Periodontal Regeneration has been reported elsewhere, the advantages of employing our bilayered PLGA + CaP construct are twofold: 1)it did not collapse into the defect; and, 2) its inner side was able to retain the blood clot throughout the buccal defect. The result was greater Periodontal Regeneration than has previously been reported with traditional flexible membranes.
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Periodontal Regeneration using a bilayered PLGA/calcium phosphate construct.
Biomaterials, 2011Co-Authors: Emily Correna Carlo Reis, Andrea Pacheco Batista Borges, Vanessa C Mendes, Limin Guan, Michel V. Furtado Araujo, John E DaviesAbstract:The Regeneration of tissues affected by Periodontal disease is a complex process; it encompasses the formation of bone, cementum and Periodontal ligament. We developed a semi-rigid PLGA (polylactide-co-glycolide acid)/CaP (calcium phosphate) bilayered biomaterial construct to promote Periodontal Regeneration, which has a continuous outer barrier membrane and an inner topographically complex component. Our experimental model compared Periodontal prophylaxis alone with prophylaxis and biomaterial implantation in the treatment of class II furcation defects in dogs. Clinical evaluation, micro-computed tomography, histology and backscattered electron imaging were used for data analysis. Healing occurred uneventfully and bone volumetric values, trabecular number and trabecular thickness were all significantly greater in the treated group; while trabecular separation was significantly greater in the control group. New cementum, bone, and Periodontal ligament with Sharpey fibre insertions were only seen in the treated group. Although Periodontal Regeneration has been reported elsewhere, the advantages of employing our bilayered PLGA + CaP construct are twofold: 1)it did not collapse into the defect; and, 2) its inner side was able to retain the blood clot throughout the buccal defect. The result was greater Periodontal Regeneration than has previously been reported with traditional flexible membranes.