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Ichiro Kamei - One of the best experts on this subject based on the ideXlab platform.
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butanol production from cellulosic material by anaerobic co culture of white rot fungus Phlebia and bacterium clostridium in consolidated bioprocessing
Bioresource Technology, 2020Co-Authors: Chu Luong Tri, Ichiro KameiAbstract:Abstract Butanol production from lignocelluloses is desirable. Unfortunately, the known wild-types of butanol fermenting Clostridium bacteria are not capable of delignification and saccharification. Here we analyzed butanol production from cellulosic material using anaerobic co-culture of C. saccharoperbutylacetonicum with the white-rot fungus Phlebia sp. MG-60-P2. In consolidated bioprocessing, the co-culture synergistically produced butanol and enhanced saccharification. Knockout of the pyruvate decarboxylase gene from MG-60-P2 to produce transformant line KO77 led to inhibition of ethanol fermentation and high accumulation of saccharified cellobiose and glucose from cellulose. In co-culture of KO77 with C. saccharoperbutylacetonicum, enhanced butanol production was observed (3.2 g/L, compared with 2.5 g/L in co-culture of MG-60-P2 and C. saccharoperbutylacetonicum). We believe this is the first application of co-culture between white-rot fungus and Clostridium to produce butanol from cellulose; butanol production from lignocellulose by co-culture of C. saccharoperbutylacetonicum with Phlebia sp. MG-60-P2 and its transformant should be pursued.
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Additional file 4: Table S3. of Analysis of ethanol fermentation mechanism of ethanol producing white-rot fungus Phlebia sp. MG-60 by RNA-seq
2016Co-Authors: Jianqiao Wang, Ichiro Kamei, Toshio Mori, Tomohiro Suzuki, Hideo Dohra, Shoko Takigami, Hiroko Kako, Ayumi Soga, Hirokazu Kawagishi, Hirofumi HiraiAbstract:Data for Phlebia sp. MG-60 transcripts. (XLSX 3497Â kb
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Additional file 7: Table S5. of Analysis of ethanol fermentation mechanism of ethanol producing white-rot fungus Phlebia sp. MG-60 by RNA-seq
2016Co-Authors: Jianqiao Wang, Ichiro Kamei, Toshio Mori, Tomohiro Suzuki, Hideo Dohra, Shoko Takigami, Hiroko Kako, Ayumi Soga, Hirokazu Kawagishi, Hirofumi HiraiAbstract:Fold changes of transcripts mapped to the glycolysis/gluconeogenesis pathway of Phlebia sp. MG-60 based on KEGG. (DOCX 35Â kb
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Additional file 2: Figure S1. of Analysis of ethanol fermentation mechanism of ethanol producing white-rot fungus Phlebia sp. MG-60 by RNA-seq
2016Co-Authors: Jianqiao Wang, Ichiro Kamei, Toshio Mori, Tomohiro Suzuki, Hideo Dohra, Shoko Takigami, Hiroko Kako, Ayumi Soga, Hirokazu Kawagishi, Hirofumi HiraiAbstract:Time courses of mycelial growth and pH changes in liquid cultures of Phlebia sp. MG-60 (A) and P. chrysosporium (B). (PPTX 153Â kb
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Additional file 6: Figure S2. of Analysis of ethanol fermentation mechanism of ethanol producing white-rot fungus Phlebia sp. MG-60 by RNA-seq
2016Co-Authors: Jianqiao Wang, Ichiro Kamei, Toshio Mori, Tomohiro Suzuki, Hideo Dohra, Shoko Takigami, Hiroko Kako, Ayumi Soga, Hirokazu Kawagishi, Hirofumi HiraiAbstract:Transcripts of Phlebia sp. MG-60 and of P. chrysosporium mapped to the glycolysis/gluconeogenesis pathway based on the KEGG pathway database. A list of the identified enzymes is provided in Tables S5 and S6. Genes showing up-regulation and down-regulation are boxed in red and green, respectively. The number of genes is shown in blue. (DOCX 161Â kb
Ryuichiro Kondo - One of the best experts on this subject based on the ideXlab platform.
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effect of chemical factors on integrated fungal fermentation of sugarcane bagasse for ethanol production by a white rot fungus Phlebia sp mg 60
Bioresource Technology, 2014Co-Authors: Ryuichiro Kondo, Rizalinda L. De Leon, To Kim Anh, Kuniyoshi Shimizu, Sadatoshi Meguro, Le Duy Khuong, Ichiro KameiAbstract:Abstract Bioethanol production through integrated fungal fermentation (IFF), involving a unified process for biological delignification with consolidated biological processing by the white-rot fungus Phlebia sp. MG-60, was applied to sugarcane bagasse. Initial moisture content of the bagasse was found to affect biological delignification by MG-60, and 75% moisture content was suitable for selective lignin degradation and subsequent ethanol production. Additives, such as basal media, organic compounds, or minerals, also affected biological delignification of bagasse by MG-60. Basal medium addition improved both delignification and ethanol production. Some inorganic chemical factors, such as Fe 2+ , Mn 2+ , or Cu 2+ , reduced bagasse carbohydrate degradation by MG-60 during delignifying incubations and resulted in increased ethanol production. The present results indicated that suitable culture conditions could significantly improve IFF efficiency.
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Bioethanol production from alkaline-pretreated sugarcane bagasse by consolidated bioprocessing using Phlebia sp. MG-60
International Biodeterioration & Biodegradation, 2014Co-Authors: Le Duy Khuong, Ryuichiro Kondo, Rizalinda L. De Leon, To Kim Anh, Kuniyoshi Shimizu, Ichiro KameiAbstract:Abstract Optimization of alkaline pretreatment of sugarcane bagasse for consolidated bioprocessing fermentation by the cellulose-fermenting fungus Phlebia sp. MG-60 was studied. The lignin and xylan contents of bagasse were decreased and ethanol production from each pretreated sugarcane bagasse by MG-60 was increased in an alkaline concentration-dependent manner. The fungus produced cellulase and xylanase rapidly over 120 h. When this fungus was cultured with 20 g L −1 of sugarcane bagasse pretreated with NaOH (0.8 wt%, 121 °C, 60 min), 4.5 g L −1 ethanol was produced, equivalent to 210 mg ethanol per gram of the original untreated bagasse after 240 h fermentation, giving ethanol yields of 65.7% of the theoretical maximum. These data suggest that Phlebia sp. MG-60 is a potential candidate for ethanol production from alkali-pretreated bagasse in a single bioreactor, without enzymatic or chemical hydrolysis.
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direct ethanol production from cellulosic materials by the hypersaline tolerant white rot fungus Phlebia sp mg 60
Bioresource Technology, 2012Co-Authors: Ichiro Kamei, Toshio Mori, Yoshiyuki Hirota, Hirofumi Hirai, Sadatoshi Meguro, Ryuichiro KondoAbstract:Abstract White-rot fungus Phlebia sp. MG-60 was identified as a good producer of ethanol from several cellulosic materials containing lignin. When this fungus was cultured with 20 g/L unbleached hardwood kraft pulp (UHKP), 8.4 g/L ethanol was produced after 168 h of incubation giving yields of ethanol of 0.42 g/g UHKP, 71.8% of the theoretical maximum. When this fungus was cultured with waste newspaper, 4.2 g/L ethanol was produced after 216 h of incubation giving yields of ethanol of 0.20 g/g newspaper, 51.1% of the theoretical maximum. Glucose, mannose, galactose, fructose and xylose were completely assimilated by Phlebia sp. MG-60 with ethanol yields of 0.44, 0.41, 0.40, 0.41 and 0.33 g/g of sugar respectively. These results indicated that Phlebia sp. MG-60 was a good candidate for bioethanol production from cellulosic materials.
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novel metabolic pathways of organochlorine pesticides dieldrin and aldrin by the white rot fungi of the genus Phlebia
Chemosphere, 2011Co-Authors: Pengfei Xiao, Ichiro Kamei, Kazuhiro Takagi, Hiromasa Kiyota, Toshio Mori, Ryuichiro KondoAbstract:White rot fungi can degrade a wide spectrum of recalcitrant organic pollutants, including polychlorinated dibenzo-p-dioxins (PCDDs) and polychlorinated biphenyls (PCBs). In this experiment, 20 white rot fungi, belonging to genus Phlebia, were investigated for their ability to degrade dieldrin. Based on the screening results, we further investigated Phlebia acanthocystis, Phlebia brevispora, and Phlebia aurea to determine their degradation capacity and metabolic products towards dieldrin and aldrin. The three fungi were able to remove over 50% of dieldrin in a low nitrogen medium, after 42 d of incubation. Three hydroxylated products were detected as metabolites of dieldrin, suggesting that in Phlebia strains, hydroxylation reactions might play an important role in the metabolism of dieldrin. In contrast to dieldrin, aldrin exhibited higher levels of degradation activity. Over 90% of aldrin was removed after 28 d of incubation, and several new metabolites of aldrin in microorganisms, including 9-hydroxyaldrin and two carboxylic acid products, were detected in fungal cultures. These results indicate that the methylene moiety of aldrin and dieldrin molecules might be prone to enzymatic attack by white rot fungi. In this study, we describe for the first time a new metabolic pathway of both compounds by fungi of genus Phlebia.
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a novel metabolic pathway for biodegradation of ddt by the white rot fungi Phlebia lindtneri and Phlebia brevispora
Biodegradation, 2011Co-Authors: Pengfei Xiao, Ichiro Kamei, Toshio Mori, Ryuichiro KondoAbstract:1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (DDT) was used as the substrate for a degradation experiment with the white rot fungi Phlebia lindtneri GB-1027 and Phlebia brevispora TMIC34596, which are capable of degrading polychlorinated dibenzo-p-dioxin (PCDD) and polychlorinated biphenyls (PCBs). Pure culture of P. lindtneri and P. brevispora with DDT (25 μmol l−1) showed that 70 and 30% of DDT, respectively, disappeared in a low-nitrogen medium after a 21-day incubation period. The metabolites were analyzed using gas chromatography/mass spectrometry (GC/MS). Both fungi metabolized DDT to 1,1-dichloro-2,2-bis(4-chlorophenyl)ethane (DDD), 2,2-bis(4-chlorophenyl)acetic acid (DDA) and 4,4-dichlorobenzophenone (DBP). Additionally, DDD was converted to DDA and DBP. DDA was converted to DBP and 4,4-dichlorobenzhydrol (DBH). While DBP was treated as substrate, DBH and three hydroxylated metabolites, including one dihydroxylated DBP and two different isomers of monohydroxylated DBH, were produced from fungal cultures, and these hydroxylated metabolites were efficiently inhibited by the addition of a cytochrome P-450 inhibitor, piperonyl butoxide. These results indicate that the white rot fungi P. lindtneri and P. brevispora can degrade DBP/DBH through hydroxylation of the aromatic ring. Moreover, the single-ring aromatic metabolites, such as 4-chlorobenzaldehyde, 4-chlorobenzyl alcohol and 4-chlorobenzoic acid, were found as metabolic products of all substrate, demonstrating that the cleavage reaction of the aliphatic-aryl carbon bond occurs in the biodegradation process of DDT by white rot fungi.
Toshio Mori - One of the best experts on this subject based on the ideXlab platform.
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Additional file 4: Table S3. of Analysis of ethanol fermentation mechanism of ethanol producing white-rot fungus Phlebia sp. MG-60 by RNA-seq
2016Co-Authors: Jianqiao Wang, Ichiro Kamei, Toshio Mori, Tomohiro Suzuki, Hideo Dohra, Shoko Takigami, Hiroko Kako, Ayumi Soga, Hirokazu Kawagishi, Hirofumi HiraiAbstract:Data for Phlebia sp. MG-60 transcripts. (XLSX 3497Â kb
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Additional file 7: Table S5. of Analysis of ethanol fermentation mechanism of ethanol producing white-rot fungus Phlebia sp. MG-60 by RNA-seq
2016Co-Authors: Jianqiao Wang, Ichiro Kamei, Toshio Mori, Tomohiro Suzuki, Hideo Dohra, Shoko Takigami, Hiroko Kako, Ayumi Soga, Hirokazu Kawagishi, Hirofumi HiraiAbstract:Fold changes of transcripts mapped to the glycolysis/gluconeogenesis pathway of Phlebia sp. MG-60 based on KEGG. (DOCX 35Â kb
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Additional file 2: Figure S1. of Analysis of ethanol fermentation mechanism of ethanol producing white-rot fungus Phlebia sp. MG-60 by RNA-seq
2016Co-Authors: Jianqiao Wang, Ichiro Kamei, Toshio Mori, Tomohiro Suzuki, Hideo Dohra, Shoko Takigami, Hiroko Kako, Ayumi Soga, Hirokazu Kawagishi, Hirofumi HiraiAbstract:Time courses of mycelial growth and pH changes in liquid cultures of Phlebia sp. MG-60 (A) and P. chrysosporium (B). (PPTX 153Â kb
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Additional file 6: Figure S2. of Analysis of ethanol fermentation mechanism of ethanol producing white-rot fungus Phlebia sp. MG-60 by RNA-seq
2016Co-Authors: Jianqiao Wang, Ichiro Kamei, Toshio Mori, Tomohiro Suzuki, Hideo Dohra, Shoko Takigami, Hiroko Kako, Ayumi Soga, Hirokazu Kawagishi, Hirofumi HiraiAbstract:Transcripts of Phlebia sp. MG-60 and of P. chrysosporium mapped to the glycolysis/gluconeogenesis pathway based on the KEGG pathway database. A list of the identified enzymes is provided in Tables S5 and S6. Genes showing up-regulation and down-regulation are boxed in red and green, respectively. The number of genes is shown in blue. (DOCX 161Â kb
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direct ethanol production from cellulosic materials by the hypersaline tolerant white rot fungus Phlebia sp mg 60
Bioresource Technology, 2012Co-Authors: Ichiro Kamei, Toshio Mori, Yoshiyuki Hirota, Hirofumi Hirai, Sadatoshi Meguro, Ryuichiro KondoAbstract:Abstract White-rot fungus Phlebia sp. MG-60 was identified as a good producer of ethanol from several cellulosic materials containing lignin. When this fungus was cultured with 20 g/L unbleached hardwood kraft pulp (UHKP), 8.4 g/L ethanol was produced after 168 h of incubation giving yields of ethanol of 0.42 g/g UHKP, 71.8% of the theoretical maximum. When this fungus was cultured with waste newspaper, 4.2 g/L ethanol was produced after 216 h of incubation giving yields of ethanol of 0.20 g/g newspaper, 51.1% of the theoretical maximum. Glucose, mannose, galactose, fructose and xylose were completely assimilated by Phlebia sp. MG-60 with ethanol yields of 0.44, 0.41, 0.40, 0.41 and 0.33 g/g of sugar respectively. These results indicated that Phlebia sp. MG-60 was a good candidate for bioethanol production from cellulosic materials.
Annele Hatakka - One of the best experts on this subject based on the ideXlab platform.
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conversion of milled pine wood by manganese peroxidase from Phlebia radiata
Applied and Environmental Microbiology, 2001Co-Authors: Martin Hofrichter, Taina Lundell, Annele HatakkaAbstract:Purified manganese peroxidase (MnP) from the white-rot basidiomycete Phlebia radiata was found to convert in vitro milled pine wood (MPW) suspended in an aqueous reaction solution containing Tween 20, Mn2+, Mn-chelating organic acid (malonate), and a hydrogen peroxide-generating system (glucose-glucose oxidase). The enzymatic attack resulted in the polymerization of lower-molecular-mass, soluble wood components and in the partial depolymerization of the insoluble bulk of pine wood, as demonstrated by high-performance size exclusion chromatography (HPSEC). The surfactant Tween 80 containing unsaturated fatty acid redsidues promoted the disintegration of bulk MPW. HPSEC showed that the depolymerization yielded preferentially lignocellulose fragments with a predominant molecular mass of ca. 0.5 kDa. MnP from P. radiata (MnP3) turned out to be a stable enzyme remaining active for 2 days even at 37°C with vigorous stirring, and 65 and 35% of the activity applied was retained in Tween 20 and Tween 80 reaction mixtures, respectively. In the course of reactions, major part of the Mn-chelator malonate was decomposed (85 to 87%), resulting in an increase of pH from 4.4 to >6.5. An aromatic nonphenolic lignin structure (β-O-4 dimer), which is normally not attacked by MnP, was oxidizible in the presence of pine wood meal. This finding indicates that certain wood components may promote the degradative activities of MnP in a way similar to that promoted by Tween 80, unsaturated fatty acids, or thiols.
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coupling of manganese peroxidase mediated lipid peroxidation with destruction of nonphenolic lignin model compounds and 14c labeled lignins
Biochemical and Biophysical Research Communications, 1999Co-Authors: Alexander N Kapich, Martin Hofrichter, Tamara Vares, Annele HatakkaAbstract:Abstract Linoleic acid, the predominant unsaturated fatty acid (UFA) in the lipids of wood-rotting fungi, was oxidized by manganese peroxidase (MnP) from the white-rot fungus Phlebia radiata through a peroxidation mechanism. The peroxidation was markedly stimulated by hydrogen peroxide. UFAs that are substrates for lipid peroxidation and surfactants that emulsify water-insoluble components were essential for the MnP-catalyzed destruction of a nonphenolic β-O-4-linked lignin model compound (LMC). Moreover, both components stimulated the MnP-catalyzed mineralization of 14 C-labeled synthetic lignin and 14 C-labeled wheat straw. A high level of destruction was obtained in reaction systems with Tween 80 acting both as surfactant and source of UFAs. The presence of the linoleic acid in reaction systems with MnP and Tween 80 additionally enhanced rate and level of LMC destruction and lignin mineralization. The results indicate that lipid peroxidation may play an important role in lignin biodegradation by wood-rotting basidiomycetes and support the hypothesis of coupling between the processes.
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oxidative decomposition of malonic acid as basis for the action of manganese peroxidase in the absence of hydrogen peroxide
FEBS Letters, 1998Co-Authors: Martin Hofrichter, Wolfgang Fritsche, D Ziegenhagen, Tamara Vares, M Friedrich, Marcus Jager, Annele HatakkaAbstract:Manganese peroxidase (MnP) from the ligninolytic basidiomycetes Phlebia radiata and Nematoloma frowardii was found to decompose malonate oxidatively in the absence of H2O2 in a reaction system consisting of the enzyme, sodium malonate and MnCl2. The enzymatic oxidation resulted in a substantial decrease in malonate concentration and the formation of CO2, oxalate, glyoxylate and formate. Simultaneously with the decomposition of malonate, Mn(II) was oxidized to Mn(III) leading to high transient concentrations of the latter. MnP action in the absence of H2O2 started slowly after a lag period of 3 h. The lag period was considerably shortened after a single addition of Mn(III). Superoxide dismutase and catalase inhibited the enzymatic reaction partly, ascorbate completely. ESR studies demonstrated the formation of a carbon-centered radical during the course of the reaction. We propose that the latter generates peroxides that can be used by MnP to oxidize Mn(II) to Mn(III).
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lignin modifying enzymes from selected white rot fungi production and role from in lignin degradation
Fems Microbiology Reviews, 1994Co-Authors: Annele HatakkaAbstract:Abstract White-rot fungi produce extracellular lignin-modifying enzymes, the best characterized of which are laccase (EC 1.10.3.2), lignin peroxidases (EC 1.11.1.7) and manganese peroxidases (EC 1.11.1.7). Lignin biodegradation studies have been carried out mostly using the white-rot fungus Phanerochaete chrysosporium which produces multiple isoenzymes of lignin peroxidase and manganese peroxidase but does not produce laccase. Many other white-rot fungi produce laccase in addition to lignin and manganese peroxidases and in varying combinations. Based on the enzyme production patterns of an array of white-rot fungi, three categories of fungi are suggested: (i) lignin-manganese peroxidase group (e.g.P. chrysosporium and Phlebia radiata), (ii) manganese peroxidase-laccase group (e.g. Dichomitus squalens and Rigidoporus lignosus), and (iii) lignin peroxidase-laccase group (e.g. Phlebia ochraceofulva and Junghuhnia separabilima). The most efficient lignin degraders, estimated by 14CO2 evolution from 14C-[Ring]-labelled synthetic lignin (DHP), belong to the first group, whereas many of the most selective lignin-degrading fungi belong to the second, although only moderate to good [14C]DHP mineralization is obtained using fungi from this group. The lignin peroxidase-laccase fungi only poorly degrade [14C]DHP.
Hirofumi Hirai - One of the best experts on this subject based on the ideXlab platform.
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Additional file 7: Table S5. of Analysis of ethanol fermentation mechanism of ethanol producing white-rot fungus Phlebia sp. MG-60 by RNA-seq
2016Co-Authors: Jianqiao Wang, Ichiro Kamei, Toshio Mori, Tomohiro Suzuki, Hideo Dohra, Shoko Takigami, Hiroko Kako, Ayumi Soga, Hirokazu Kawagishi, Hirofumi HiraiAbstract:Fold changes of transcripts mapped to the glycolysis/gluconeogenesis pathway of Phlebia sp. MG-60 based on KEGG. (DOCX 35Â kb
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Additional file 4: Table S3. of Analysis of ethanol fermentation mechanism of ethanol producing white-rot fungus Phlebia sp. MG-60 by RNA-seq
2016Co-Authors: Jianqiao Wang, Ichiro Kamei, Toshio Mori, Tomohiro Suzuki, Hideo Dohra, Shoko Takigami, Hiroko Kako, Ayumi Soga, Hirokazu Kawagishi, Hirofumi HiraiAbstract:Data for Phlebia sp. MG-60 transcripts. (XLSX 3497Â kb
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Additional file 2: Figure S1. of Analysis of ethanol fermentation mechanism of ethanol producing white-rot fungus Phlebia sp. MG-60 by RNA-seq
2016Co-Authors: Jianqiao Wang, Ichiro Kamei, Toshio Mori, Tomohiro Suzuki, Hideo Dohra, Shoko Takigami, Hiroko Kako, Ayumi Soga, Hirokazu Kawagishi, Hirofumi HiraiAbstract:Time courses of mycelial growth and pH changes in liquid cultures of Phlebia sp. MG-60 (A) and P. chrysosporium (B). (PPTX 153Â kb
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Additional file 6: Figure S2. of Analysis of ethanol fermentation mechanism of ethanol producing white-rot fungus Phlebia sp. MG-60 by RNA-seq
2016Co-Authors: Jianqiao Wang, Ichiro Kamei, Toshio Mori, Tomohiro Suzuki, Hideo Dohra, Shoko Takigami, Hiroko Kako, Ayumi Soga, Hirokazu Kawagishi, Hirofumi HiraiAbstract:Transcripts of Phlebia sp. MG-60 and of P. chrysosporium mapped to the glycolysis/gluconeogenesis pathway based on the KEGG pathway database. A list of the identified enzymes is provided in Tables S5 and S6. Genes showing up-regulation and down-regulation are boxed in red and green, respectively. The number of genes is shown in blue. (DOCX 161Â kb
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direct ethanol production from cellulosic materials by the hypersaline tolerant white rot fungus Phlebia sp mg 60
Bioresource Technology, 2012Co-Authors: Ichiro Kamei, Toshio Mori, Yoshiyuki Hirota, Hirofumi Hirai, Sadatoshi Meguro, Ryuichiro KondoAbstract:Abstract White-rot fungus Phlebia sp. MG-60 was identified as a good producer of ethanol from several cellulosic materials containing lignin. When this fungus was cultured with 20 g/L unbleached hardwood kraft pulp (UHKP), 8.4 g/L ethanol was produced after 168 h of incubation giving yields of ethanol of 0.42 g/g UHKP, 71.8% of the theoretical maximum. When this fungus was cultured with waste newspaper, 4.2 g/L ethanol was produced after 216 h of incubation giving yields of ethanol of 0.20 g/g newspaper, 51.1% of the theoretical maximum. Glucose, mannose, galactose, fructose and xylose were completely assimilated by Phlebia sp. MG-60 with ethanol yields of 0.44, 0.41, 0.40, 0.41 and 0.33 g/g of sugar respectively. These results indicated that Phlebia sp. MG-60 was a good candidate for bioethanol production from cellulosic materials.
