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Michael J. Ryan - One of the best experts on this subject based on the ideXlab platform.

  • risky ripples allow bats and frogs to eavesdrop on a multisensory sexual display
    Science, 2014
    Co-Authors: Michael J. Ryan, Wouter Halfwerk, Patricia L Jones, Ryan C Taylor, Rachel A Page
    Abstract:

    Animal displays are often perceived by intended and unintended receivers in more than one sensory system. In addition, cues that are an incidental consequence of signal production can also be perceived by different receivers, even when the receivers use different sensory systems to perceive them. Here we show that the vocal responses of male tungara frogs (Physalaemus pustulosus) increase twofold when call-induced water ripples are added to the acoustic component of a rival’s call. Hunting bats (Trachops cirrhosus) can echolocate this signal by-product and prefer to attack model frogs when ripples are added to the acoustic component of the call. This study illustrates how the perception of a signal by-product by intended and unintended receivers through different sensory systems generates both costs and benefits for the signaler.

  • evoked vocal responses change with experience in male Physalaemus pustulosus
    Copeia, 2012
    Co-Authors: Beth Dawson, Michael J. Ryan
    Abstract:

    The acquisition of signals used in intrasexual communication is not well studied. To better understand the possible contribution of early experience to the acquisition of intrasexual responses, we reared Neotropical Tungara frogs, Physalaemus pustulosus, in four acoustic environments: 1) conspecific chorus, 2) heterospecific chorus, 3) acoustic isolation, and 4) noise. For the heterospecific chorus, we chose the calls of a congener, P. enesefae, with which P. pustulosus is sympatric in parts of its distribution. We measured the evoked vocal responses (EVR) of these frogs in response to calls of the conspecifics, to calls of the congener, and to calls representing an intermediate between the two species. Male P. pustulosus reared hearing the calls of P. enesefae produced more calls overall and more complex calls in response to the call of P. enesefae. This is consistent with the hypothesis that the EVR of male anurans can be influenced by early experience and is the product of an interaction between genes ...

  • predation risk increases permissiveness for heterospecific advertisement calls in tungara frogs Physalaemus pustulosus
    Animal Behaviour, 2011
    Co-Authors: Luis A Bonachea, Michael J. Ryan
    Abstract:

    Factors that influence the strength of sexual selection within a population may have important consequences for the evolution and maintenance of reproductive isolation between populations. Here we examine the role of one such factor, predation risk, in influencing female permissiveness for heterospecific advertisement calls. Specifically, we tested the effect of simulated predation risk on mate choice in female tungara frogs, Physalaemus pustulosus, using variation in ambient light levels, travel time and the presence of auditory cues of local predators. Simulated predation risk increased female permissiveness for advertisement calls along an artificial gradient of calls intermediate between conspecifics and a congener, Physalaemus enesefae. Across the entire gradient, the presence of auditory cues of the predatory frog Leptodactylus pentadactylus in association with the conspecific call dramatically increased the likelihood of females choosing the intermediate call. In addition, higher ambient light levels and simulation of increased travel distances both increased the likelihood that females would choose intermediate calls over conspecific calls. These results suggest that, although mate choice may be important in causing reproductive isolation between allopatric populations, spatial or temporal variation in predation risk may strongly influence the expression of mate choice and thus the outcome of secondary contact.

  • Temporal updating during phonotaxis in male túngara frogs (Physalaemus pustulosus)
    Amphibia-Reptilia, 2010
    Co-Authors: Alexander T. Baugh, Michael J. Ryan
    Abstract:

    AbstractIn acoustically advertising anurans the male courtship call elicits species-typical responses from conspecifics – usually phonotactic approach and mate choice in gravid females and an evoked vocal response in adult males. Males in several species, however, are also known to perform phonotaxis, sometimes with the same acoustic preferences as females. Female túngara frogs are known to update their phonotactic approach as male advertisement signals change dynamically in attractiveness. Here we show that males also perform such temporal updating during phonotaxis in response to dynamic playbacks. While males exhibit slower phonotactic approaches than females, their responsiveness to dynamic changes in call complexity does not differ significantly compared to females. These results demonstrate that males are sensitive to the location of preferred call types on a moment-to-moment basis and suggest that similarities between male and female sexual behaviour in anurans might often be overlooked. We suggest that anuran phonotaxis is more widespread and serves different functions in reproductive females and males. Lastly, these temporal updating results suggest that male frogs are highly selective about site selection in a chorus.

  • The development of sexual behavior in túngara frogs (Physalaemus pustulosus).
    Journal of comparative psychology (Washington D.C. : 1983), 2010
    Co-Authors: Alexander T. Baugh, Michael J. Ryan
    Abstract:

    We examined the emergence of a critical component of sex, response to sexual signals-phonotaxis-in male and female tungara frogs (Physalaemus pustulosus). We determined the ontogenetic trajectories of phonotactic responses as animals developed from metamorphic froglets to reproductive adults. The results demonstrated that species-typical phonotaxis emerges quite early during postmetamorphic development, well before sexual maturity, suggesting that a developmentally early bias in the auditory system for species-typical signals might be a more general phenomenon than previously thought, and that the neural circuits responsible for processing and responding to conspecific advertisement signals in a species-typical manner might develop long before the coordinated behavior is demanded of the organism.

Luciana Bolsoni Lourenco - One of the best experts on this subject based on the ideXlab platform.

  • amphibians from serra do cipo minas gerais brasil vi a new species of the physalemus deimaticus group anura leptodactylidae
    Zootaxa, 2020
    Co-Authors: Fernando Leal, Luciana Bolsoni Lourenco, Luciana B Nascimento, Felipe Sa Fortes Leite, William Pinheiro Da Costa, Paulo C A Garcia
    Abstract:

    We describe a new species of Physalaemus assigned to the Physalaemus signifer Clade, and it is morphologically similar to P. rupestris, from the highlands of the Serra do Cipo in the southern Espinhaco Range, State of Minas Gerais, Southeastern Brazil. The new species is diagnosed by using the following combination of character states: presence of an arrow-shaped blotch on the dorsum of body; presence of a median longitudinal light stripe over urostyle region; belly and ventral surface of thigh marbled with dark gray irregularly shaped blotches on a bluish background; absence of reddish coloration over axillary and inguinal regions in live individuals; bright orange dots scattered over head, upper lip, and dorsum of body in live individuals; ventral surface of hand and foot red in live individuals; small size (adult male SVL=16.2-18.2 mm); presence of brown, not divided, nuptial pad in males; END/ED in males ranging from 0.85-0.93; supernumerary tubercles on foot absent; tarsal fold absent; tarsal tubercle absent; texture of posterior region of belly and ventral surface of thigh smooth; advertisement call composed of two note types (note A + B); advertisement call duration of 0.80-1.28 s; note A with ascending amplitude until mid-note then descending towards the end of the note; note B with pulses arranged in 5-7 groups; and dominant frequency of note A from 1734.4-2765.6 Hz and of note B from 1507.3-2859.4 Hz. A phylogenetic analysis based on mitochondrial DNA sequences recovered the new species nested within the Physalaemus deimaticus species group. Additionally, we redescribe the call of Physalaemus rupestris and provide a review of the geographic distribution and conservation status of the species belonging to the P. deimaticus species group.

  • extensive cryptic diversity within the Physalaemus cuvieri Physalaemus ephippifer species complex amphibia anura revealed by cytogenetic mitochondrial and genomic markers
    Frontiers in Genetics, 2019
    Co-Authors: Juliana Damieli Nascimento, Diego Baldo, Shirlei Maria Reccopimentel, Gilda V Andrade, Celio F B Haddad, Jucivaldo Dias Lima, Pablo Suarez, Todd W Pierson, Benjamin M Fitzpatrick, Luciana Bolsoni Lourenco
    Abstract:

    Previous cytogenetic and phylogenetic analyses showed a high variability in the frog taxa Physalaemus cuvieri and Physalaemus ephippifer and suggested the presence of undescribed diversity in this species complex. Here, by 1) adding specimens from the Brazilian Amazon region, 2) employing sequence-based species delimitation approaches, and 3) including RADseq-style markers, we demonstrate that the diversity in the P. cuvieri – P. ephippifer species complex is even greater than previously suspected. Specimens from Virua and Western Para, located at the Guiana Amazonian area of endemism, were recovered as distinct from all previously identified lineages by the phylogenetic analyses based on mitochondrial DNA and RAD markers, a PCA from RAD data, and cytogenetic analysis. The sequence-based species delimitation analyses supported the recognition of one or two undescribed species among these Amazonian specimens and also supported the recognition of at least three other species in the P. cuvieri – P. ephippifer species complex. These new results reinforce the need for a comprehensive taxonomic revision.

  • first insights on the retroelement rex1 in the cytogenetics of frogs
    Molecular Cytogenetics, 2015
    Co-Authors: Juliana M Nascimento, Diego Baldo, Luciana Bolsoni Lourenco
    Abstract:

    Background While some transposable elements (TEs) have been found in the sequenced genomes of frog species, detailed studies of these elements have been lacking. In this work, we investigated the occurrence of the Rex1 element, which is widespread in fish, in anurans of the genus Physalaemus. We isolated and characterized the reverse transcriptase (RT)-coding sequences of Rex1 elements of five species of this genus.

  • phylogeny of frogs from the genus Physalaemus anura leptodactylidae inferred from mitochondrial and nuclear gene sequences
    Molecular Phylogenetics and Evolution, 2015
    Co-Authors: Luciana Bolsoni Lourenco, Diego Baldo, Cintia Pelegrineti Targueta, Juliana Damieli Nascimento, Paulo C A Garcia, Gilda V Andrade, Celio F B Haddad, Shirlei Maria Reccopimentel
    Abstract:

    Although some species groups have been recognized in the leiuperine genus Physalaemus, no phylogenetic analysis has previously been performed. Here, we provide a phylogenetic study based on mitochondrial and nuclear DNA sequences from 41 of the 46 species of Physalaemus. We employed the parsimony criterion using the software TNT and POY and the Bayesian criterion using the software MrBayes. Two major clades were recovered inside the monophyletic Physalaemus: (i) the highly supported Physalaemus signifer Clade, which included P. nattereri and the species previously placed in the P. deimaticus and P. signifer Groups; and (ii) the Physalaemus cuvieri Clade, which included the remaining species of Physalaemus. Five species groups were recognized in the P. cuvieri Clade: the P. biligonigerus Group, the P. cuvieri Group, the P. henselii Group, the P. gracilis Group and the P. olfersii Group. The P. gracilis Species Group was the same as that previously proposed by Nascimento et al. (2005). The P. henselii Group includes P. fernandezae and P. henselii, and was the sister group of a clade that comprised the remaining species of the P. cuvieri Clade. The P. olfersii Group included P. olfersii, P. soaresi, P. maximus, P. feioi and P. lateristriga. The P. biligonigerus Species Group was composed of P. biligonigerus, P. marmoratus, P. santafecinus and P. riograndensis. The P. cuvieri Group inferred here differed from that recognized by Nascimento et al. (2005) only by the inclusion of P. albifrons and the exclusion of P. cicada. The paraphyly of P. cuvieri with respect to P. ephippifer was inferred in all the analyses. Distinct genetic lineages were recognized among individuals currently identified as P. cuvieri and they were congruent with cytogenetic differences reported previously, supporting the hypothesis of occurrence of formally unnamed species.

  • comparative cytogenetics of Physalaemus albifrons and Physalaemus cuvieri species groups anura leptodactylidae
    Comparative Cytogenetics, 2014
    Co-Authors: Stenio Eder Vittorazzi, Diego Baldo, Yrsd Quindere, Shirlei Maria Reccopimentel, Cristian Tomatis, Janaina Reis, Juan Martin Ferro, Jucivaldo Lima, Luciana Bolsoni Lourenco
    Abstract:

    Recently, Physalaemus albifrons (Spix, 1824) was relocated from the Physalaemus cuvieri group to the same group as Physalaemus biligonigerus (Cope, 1861), Physalaemus marmoratus (Reinhardt & Lutken, 1862) and Physalaemus santafecinus Barrio, 1965. To contribute to the analysis of this proposition, we studied the karyotypes of Physalaemus albifrons, Physalaemus santafecinus and three species of the Physalaemus cuvieri group. The karyotype of Physalaemus santafecinus was found to be very similar to those of Physalaemus biligonigerus and Physalaemus marmoratus, which were previously described. A remarkable characteristic that these three species share is a conspicuous C-band that extends from the pericentromeric region almost to the telomere in the short arm of chromosome 3. This characteristic is not present in the Physalaemus albifrons karyotype and could be a synapomorphy of Physalaemus biligonigerus, Physalaemus marmoratus and Physalaemus santafecinus. The karyotype of Physalaemus santafecinus is also similar to those of Physalaemus marmoratus and Physalaemus biligonigerus owing to the presence of several terminal C-bands and the distal localization of the NOR in a small metacentric chromosome. In contrast, the Physalaemus albifrons karyotype has no terminal C-bands and its NOR is located interstitially in the long arm of submetacentric chromosome 8. The NOR-bearing chromosome of Physalaemus albifrons very closely resembles those found in Physalaemus albonotatus (Steindachner, 1864), Physalaemus cuqui Lobo, 1993 and some populations of Physalaemus cuvieri Fitzinger, 1826. Additionally, the Physalaemus albifrons karyotype has an interstitial C-band in chromosome 5 that has been exclusively observed in species of the Physalaemus cuvieri group. Therefore, we were not able to identify any chromosomal feature that supports the reallocation of Physalaemus albifrons.

Diego Baldo - One of the best experts on this subject based on the ideXlab platform.

  • extensive cryptic diversity within the Physalaemus cuvieri Physalaemus ephippifer species complex amphibia anura revealed by cytogenetic mitochondrial and genomic markers
    Frontiers in Genetics, 2019
    Co-Authors: Juliana Damieli Nascimento, Diego Baldo, Shirlei Maria Reccopimentel, Gilda V Andrade, Celio F B Haddad, Jucivaldo Dias Lima, Pablo Suarez, Todd W Pierson, Benjamin M Fitzpatrick, Luciana Bolsoni Lourenco
    Abstract:

    Previous cytogenetic and phylogenetic analyses showed a high variability in the frog taxa Physalaemus cuvieri and Physalaemus ephippifer and suggested the presence of undescribed diversity in this species complex. Here, by 1) adding specimens from the Brazilian Amazon region, 2) employing sequence-based species delimitation approaches, and 3) including RADseq-style markers, we demonstrate that the diversity in the P. cuvieri – P. ephippifer species complex is even greater than previously suspected. Specimens from Virua and Western Para, located at the Guiana Amazonian area of endemism, were recovered as distinct from all previously identified lineages by the phylogenetic analyses based on mitochondrial DNA and RAD markers, a PCA from RAD data, and cytogenetic analysis. The sequence-based species delimitation analyses supported the recognition of one or two undescribed species among these Amazonian specimens and also supported the recognition of at least three other species in the P. cuvieri – P. ephippifer species complex. These new results reinforce the need for a comprehensive taxonomic revision.

  • first insights on the retroelement rex1 in the cytogenetics of frogs
    Molecular Cytogenetics, 2015
    Co-Authors: Juliana M Nascimento, Diego Baldo, Luciana Bolsoni Lourenco
    Abstract:

    Background While some transposable elements (TEs) have been found in the sequenced genomes of frog species, detailed studies of these elements have been lacking. In this work, we investigated the occurrence of the Rex1 element, which is widespread in fish, in anurans of the genus Physalaemus. We isolated and characterized the reverse transcriptase (RT)-coding sequences of Rex1 elements of five species of this genus.

  • phylogeny of frogs from the genus Physalaemus anura leptodactylidae inferred from mitochondrial and nuclear gene sequences
    Molecular Phylogenetics and Evolution, 2015
    Co-Authors: Luciana Bolsoni Lourenco, Diego Baldo, Cintia Pelegrineti Targueta, Juliana Damieli Nascimento, Paulo C A Garcia, Gilda V Andrade, Celio F B Haddad, Shirlei Maria Reccopimentel
    Abstract:

    Although some species groups have been recognized in the leiuperine genus Physalaemus, no phylogenetic analysis has previously been performed. Here, we provide a phylogenetic study based on mitochondrial and nuclear DNA sequences from 41 of the 46 species of Physalaemus. We employed the parsimony criterion using the software TNT and POY and the Bayesian criterion using the software MrBayes. Two major clades were recovered inside the monophyletic Physalaemus: (i) the highly supported Physalaemus signifer Clade, which included P. nattereri and the species previously placed in the P. deimaticus and P. signifer Groups; and (ii) the Physalaemus cuvieri Clade, which included the remaining species of Physalaemus. Five species groups were recognized in the P. cuvieri Clade: the P. biligonigerus Group, the P. cuvieri Group, the P. henselii Group, the P. gracilis Group and the P. olfersii Group. The P. gracilis Species Group was the same as that previously proposed by Nascimento et al. (2005). The P. henselii Group includes P. fernandezae and P. henselii, and was the sister group of a clade that comprised the remaining species of the P. cuvieri Clade. The P. olfersii Group included P. olfersii, P. soaresi, P. maximus, P. feioi and P. lateristriga. The P. biligonigerus Species Group was composed of P. biligonigerus, P. marmoratus, P. santafecinus and P. riograndensis. The P. cuvieri Group inferred here differed from that recognized by Nascimento et al. (2005) only by the inclusion of P. albifrons and the exclusion of P. cicada. The paraphyly of P. cuvieri with respect to P. ephippifer was inferred in all the analyses. Distinct genetic lineages were recognized among individuals currently identified as P. cuvieri and they were congruent with cytogenetic differences reported previously, supporting the hypothesis of occurrence of formally unnamed species.

  • Additional file 1: of First insights on the retroelement Rex1 in the cytogenetics of frogs
    2015
    Co-Authors: Juliana Nascimento, Diego Baldo, Luciana Lourenรงo
    Abstract:

    Alignment of the fragments of the retroelement Rex1 isolated from species of Physalaemus with corresponding sequences available in GenBank and Repbase. Note that the sequence CR1 of Gallus gallus (GenBank accession number U88211.1) significantly differs from all other sequences. The primers used to isolate the sequences are indicated in gray. Black areas indicate identical sites, while variable sites are colored white. Premature stop codons are shown in blue. U18939.1 is the GenBank accession number of a Babar sequence (a Rex1-related element) of Battrachocottus baikalensis. AJ288466.1, AJ288450.1, AJ288444.1 and AJ288442.1 are GenBank accession numbers of retroelement Rex1 sequences isolated from Anguilla japonica (AJ288466.1) or Xiphophorus helleri. Xenopus tropicalis REX1-5, REX1-2 and REX1-3 are sequences isolated from Xenopus tropicalis and available at the Repbase database ( http://www.girinst.org/censor/index.php ). (DOCX 33 kb

  • comparative cytogenetics of Physalaemus albifrons and Physalaemus cuvieri species groups anura leptodactylidae
    Comparative Cytogenetics, 2014
    Co-Authors: Stenio Eder Vittorazzi, Diego Baldo, Yrsd Quindere, Shirlei Maria Reccopimentel, Cristian Tomatis, Janaina Reis, Juan Martin Ferro, Jucivaldo Lima, Luciana Bolsoni Lourenco
    Abstract:

    Recently, Physalaemus albifrons (Spix, 1824) was relocated from the Physalaemus cuvieri group to the same group as Physalaemus biligonigerus (Cope, 1861), Physalaemus marmoratus (Reinhardt & Lutken, 1862) and Physalaemus santafecinus Barrio, 1965. To contribute to the analysis of this proposition, we studied the karyotypes of Physalaemus albifrons, Physalaemus santafecinus and three species of the Physalaemus cuvieri group. The karyotype of Physalaemus santafecinus was found to be very similar to those of Physalaemus biligonigerus and Physalaemus marmoratus, which were previously described. A remarkable characteristic that these three species share is a conspicuous C-band that extends from the pericentromeric region almost to the telomere in the short arm of chromosome 3. This characteristic is not present in the Physalaemus albifrons karyotype and could be a synapomorphy of Physalaemus biligonigerus, Physalaemus marmoratus and Physalaemus santafecinus. The karyotype of Physalaemus santafecinus is also similar to those of Physalaemus marmoratus and Physalaemus biligonigerus owing to the presence of several terminal C-bands and the distal localization of the NOR in a small metacentric chromosome. In contrast, the Physalaemus albifrons karyotype has no terminal C-bands and its NOR is located interstitially in the long arm of submetacentric chromosome 8. The NOR-bearing chromosome of Physalaemus albifrons very closely resembles those found in Physalaemus albonotatus (Steindachner, 1864), Physalaemus cuqui Lobo, 1993 and some populations of Physalaemus cuvieri Fitzinger, 1826. Additionally, the Physalaemus albifrons karyotype has an interstitial C-band in chromosome 5 that has been exclusively observed in species of the Physalaemus cuvieri group. Therefore, we were not able to identify any chromosomal feature that supports the reallocation of Physalaemus albifrons.

Ulisses Caramaschi - One of the best experts on this subject based on the ideXlab platform.

  • taxonomic review of Physalaemus olfersii lichtenstein martens 1856 with revalidation of Physalaemus lateristriga steindachner 1864 and description of two new related species anura leiuperidae
    Zootaxa, 2010
    Co-Authors: Carla Santana Cassini, Carlos Alberto Goncalves Cruz, Ulisses Caramaschi
    Abstract:

    Physalaemus olfersii was described by M.H.K. Lichtenstein and E.K. Martens in 1856 based on two syntypes collected by I.M. Olfers between the years 1818 and 1819. Due to the brevity of it’s original description and high interpopulational variation, a taxonomic review of material assigned to the species is proposed. Morphological comparisons allowed us to associate the syntype of P. olfersii to populations distributed between the state of Espirito Santo and of Sao Paulo to which the southern limit is the to the municipality of Sao Paulo. Historical documents allowed us to define an area within Serra do Mar mountain range as the type locality of the species. Morphological and acoutics comparisons of P. olfersii from different localities in southern and southeastern Brazil revealed considerable variation among populations. Differences in call parameters and morphology, led us to conclude that three more species where under the name P. olfersii: Physalaemus lateristriga (Steindachner, 1864) comb. nov. and two new species, Physalaemus feioi sp. nov. and P. orophilus sp. nov.

  • a new species of the genus Physalaemus fitzinger 1826 anura leiuperidae from southern brazil
    South American Journal of Herpetology, 2008
    Co-Authors: Carlos Alberto Goncalves Cruz, Carla Santana Cassini, Ulisses Caramaschi
    Abstract:

    Abstract A new species of the genus Physalaemus is described from Serra da Pedra Branca do Araraquara, Municipality of Guaratuba, State of Parana, southern Brazil. Belonging to the P. olfersii species group, Physalaemus insperatus sp. nov. is closely related to P. aguirrei and presents well-developed vocal sacs expanded laterally to the supratympanic fold, digits very thin, and large inguinal glands.

  • taxonomic status of gomphobates marmoratus reinhardt and lutken 1862 1861 and eupemphix fuscomaculatus steindachner 1864 amphibia anura leptodactylidae
    South American Journal of Herpetology, 2006
    Co-Authors: Luciana B Nascimento, Bruno V S Pimenta, Carlos Alberto Goncalves Cruz, Ulisses Caramaschi
    Abstract:

    Abstract An analysis of specimens deposited in herpetological collections and identified as Physalaemus fuscomaculatus demonstrated that these differ from the types of this taxon, Eupemphix fuscomaculatus, Consequently, the taxonomic status of Gomphobates marmoratus and Eupemphix fuscomaculatus was revised based on the types and on data in the literature. Gomphobates marmoratus is associated to the genus Physalaemus, as Physalaemus marmoratus, and refers to specimens previously identified as Physalaemus fuscomaculatus, Additionally, Eupemphix fuscomaculatus is associated to the genus Pleurodema in the combination Pleurodema fuscomaculata.

  • a new brightly colored species of Physalaemus anura leptodactylidae from minas gerais southeastern brazil
    Herpetologica, 2003
    Co-Authors: Ulisses Caramaschi, Renato Neves Feio, Aristides S Guimaraesneto
    Abstract:

    A new, brightly colored species of Physalaemus similar to P. deimaticus and P. rupestris is described from the Parque Estadual do Itacolomi, Municipality of Ouro Preto, State of Minas Gerais, southeastern Brazil. The new species is characterized by its small size (snout–vent length 23.2 mm in male, 26.2–26.6 mm in females) and by having the snout rounded in the dorsal view and protruding in profile; large inguinal glands, ovoid, light brown with a black ocellus outlined with white on their posterior half; light grayish brown dorsum with elongate longitudinal dark grayish brown stripes and blotches; venter white with gray mottling; and bright red on groin and ventral surfaces of arms, hands, thighs, tibiae, and feet.

Shirlei Maria Reccopimentel - One of the best experts on this subject based on the ideXlab platform.

  • extensive cryptic diversity within the Physalaemus cuvieri Physalaemus ephippifer species complex amphibia anura revealed by cytogenetic mitochondrial and genomic markers
    Frontiers in Genetics, 2019
    Co-Authors: Juliana Damieli Nascimento, Diego Baldo, Shirlei Maria Reccopimentel, Gilda V Andrade, Celio F B Haddad, Jucivaldo Dias Lima, Pablo Suarez, Todd W Pierson, Benjamin M Fitzpatrick, Luciana Bolsoni Lourenco
    Abstract:

    Previous cytogenetic and phylogenetic analyses showed a high variability in the frog taxa Physalaemus cuvieri and Physalaemus ephippifer and suggested the presence of undescribed diversity in this species complex. Here, by 1) adding specimens from the Brazilian Amazon region, 2) employing sequence-based species delimitation approaches, and 3) including RADseq-style markers, we demonstrate that the diversity in the P. cuvieri – P. ephippifer species complex is even greater than previously suspected. Specimens from Virua and Western Para, located at the Guiana Amazonian area of endemism, were recovered as distinct from all previously identified lineages by the phylogenetic analyses based on mitochondrial DNA and RAD markers, a PCA from RAD data, and cytogenetic analysis. The sequence-based species delimitation analyses supported the recognition of one or two undescribed species among these Amazonian specimens and also supported the recognition of at least three other species in the P. cuvieri – P. ephippifer species complex. These new results reinforce the need for a comprehensive taxonomic revision.

  • phylogeny of frogs from the genus Physalaemus anura leptodactylidae inferred from mitochondrial and nuclear gene sequences
    Molecular Phylogenetics and Evolution, 2015
    Co-Authors: Luciana Bolsoni Lourenco, Diego Baldo, Cintia Pelegrineti Targueta, Juliana Damieli Nascimento, Paulo C A Garcia, Gilda V Andrade, Celio F B Haddad, Shirlei Maria Reccopimentel
    Abstract:

    Although some species groups have been recognized in the leiuperine genus Physalaemus, no phylogenetic analysis has previously been performed. Here, we provide a phylogenetic study based on mitochondrial and nuclear DNA sequences from 41 of the 46 species of Physalaemus. We employed the parsimony criterion using the software TNT and POY and the Bayesian criterion using the software MrBayes. Two major clades were recovered inside the monophyletic Physalaemus: (i) the highly supported Physalaemus signifer Clade, which included P. nattereri and the species previously placed in the P. deimaticus and P. signifer Groups; and (ii) the Physalaemus cuvieri Clade, which included the remaining species of Physalaemus. Five species groups were recognized in the P. cuvieri Clade: the P. biligonigerus Group, the P. cuvieri Group, the P. henselii Group, the P. gracilis Group and the P. olfersii Group. The P. gracilis Species Group was the same as that previously proposed by Nascimento et al. (2005). The P. henselii Group includes P. fernandezae and P. henselii, and was the sister group of a clade that comprised the remaining species of the P. cuvieri Clade. The P. olfersii Group included P. olfersii, P. soaresi, P. maximus, P. feioi and P. lateristriga. The P. biligonigerus Species Group was composed of P. biligonigerus, P. marmoratus, P. santafecinus and P. riograndensis. The P. cuvieri Group inferred here differed from that recognized by Nascimento et al. (2005) only by the inclusion of P. albifrons and the exclusion of P. cicada. The paraphyly of P. cuvieri with respect to P. ephippifer was inferred in all the analyses. Distinct genetic lineages were recognized among individuals currently identified as P. cuvieri and they were congruent with cytogenetic differences reported previously, supporting the hypothesis of occurrence of formally unnamed species.

  • comparative cytogenetics of Physalaemus albifrons and Physalaemus cuvieri species groups anura leptodactylidae
    Comparative Cytogenetics, 2014
    Co-Authors: Stenio Eder Vittorazzi, Diego Baldo, Yrsd Quindere, Shirlei Maria Reccopimentel, Cristian Tomatis, Janaina Reis, Juan Martin Ferro, Jucivaldo Lima, Luciana Bolsoni Lourenco
    Abstract:

    Recently, Physalaemus albifrons (Spix, 1824) was relocated from the Physalaemus cuvieri group to the same group as Physalaemus biligonigerus (Cope, 1861), Physalaemus marmoratus (Reinhardt & Lutken, 1862) and Physalaemus santafecinus Barrio, 1965. To contribute to the analysis of this proposition, we studied the karyotypes of Physalaemus albifrons, Physalaemus santafecinus and three species of the Physalaemus cuvieri group. The karyotype of Physalaemus santafecinus was found to be very similar to those of Physalaemus biligonigerus and Physalaemus marmoratus, which were previously described. A remarkable characteristic that these three species share is a conspicuous C-band that extends from the pericentromeric region almost to the telomere in the short arm of chromosome 3. This characteristic is not present in the Physalaemus albifrons karyotype and could be a synapomorphy of Physalaemus biligonigerus, Physalaemus marmoratus and Physalaemus santafecinus. The karyotype of Physalaemus santafecinus is also similar to those of Physalaemus marmoratus and Physalaemus biligonigerus owing to the presence of several terminal C-bands and the distal localization of the NOR in a small metacentric chromosome. In contrast, the Physalaemus albifrons karyotype has no terminal C-bands and its NOR is located interstitially in the long arm of submetacentric chromosome 8. The NOR-bearing chromosome of Physalaemus albifrons very closely resembles those found in Physalaemus albonotatus (Steindachner, 1864), Physalaemus cuqui Lobo, 1993 and some populations of Physalaemus cuvieri Fitzinger, 1826. Additionally, the Physalaemus albifrons karyotype has an interstitial C-band in chromosome 5 that has been exclusively observed in species of the Physalaemus cuvieri group. Therefore, we were not able to identify any chromosomal feature that supports the reallocation of Physalaemus albifrons.

  • chromosomal analysis of the leptodactylids pleurodema diplolistris and Physalaemus nattereri amphibia anura
    Web Science, 2006
    Co-Authors: Luciana Bolsoni Lourenco, Denise De Cerqueira Rossaferes, Gilda V Andrade, Josiane A A Nascimento, Shirlei Maria Reccopimentel
    Abstract:

    Detailed characterizations of the karyotypes of the Brazilian leptodactylid frogs Pleurodema diplolistris, the only species of Pleurodema not studied cytogenetically so far, and Physalaemus nattereri, a species in the Ph. biligonigerus group, are presented. Both karyotypes had 2n = 22 and their chromosomes had a very similar morphology, except for pair 11, which was metacentric in Pl. diplolistris and telocentric in Ph. nattereri. The localization of nucleolar organizer regions (NORs) and heterochromatic bands allowed the differentiation of chromosomes that were morphologically indistinguishable between these species, such as pairs 1, 3 and 10, which showed interstitial C-bands in Ph. nattereri, and pair 8, that had an NOR and an adjacent C-band in Pl. diplolistris. Pair 8 also has NOR-bearing chromosomes in many other Pleurodema species. However, in these species, the NOR is located proximal to the centromere on the short arm, while in Pl. diplolistris it occurred distally on the long arm, a condition that may be considered a derived state. In Ph. nattereri, the NOR occurred on chromosome 11 and differed from the other species of the Ph. biligonigerus group. In contrast, C-banding revealed a heterochromatic block near the centromere on the short arm of pair 3, a characteristic common to all members of this group of Physalaemus.

  • cytogenetic analysis of three Physalaemus species amphibia anura
    Caryologia, 2000
    Co-Authors: Mauricio José Lopes Vaz Do Amaral, Adao J Cardoso, Shirlei Maria Reccopimentel
    Abstract:

    Abstract Physalaemus biligonigerus, P. fuscomaculatus and P. sp. have 2n=2 chromosomes, consistent with the number of chromosomal pairs found in the genus.There were no substantial differences in the karyotypes of the three species, except for chromosome 3 which was submetacentric in P. fuscomaculatus and metacentric in P. biligonigerus and P. sp.All chromosomes were characterised by the presence of a C-band in the pericentromeric region. Some interstitial bands were also detected at the same position in all karyotypes, but the band located on the short arm of chromosome 3 was larger in P.biligonigerus and P. sp. than in P. fuscomaculatus.Also, only P. sp. had a large block of pericentromeric heterochro-matin in the short arm of chromosome 9 and another one in the telomeric band on the long arm. The NOR was located on the long arm of chromosome 9 in the three species. These findings confirmed that the three species are indeed closely related cytogenetically.