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Mitsuro Hyakumachi - One of the best experts on this subject based on the ideXlab platform.

  • role of ethylene signalling in growth and systemic resistance induction by the plant growth Promoting Fungus penicillium viridicatum in arabidopsis
    Journal of Phytopathology, 2017
    Co-Authors: Md Motaher Hossain, Farjana Sultana, Mitsuro Hyakumachi
    Abstract:

    The plant growth-Promoting fungi (PGPF) have long been known to improve plant growth and suppress plant diseases. The PGPF Penicillium viridicatum GP15-1 elicited plant growth and induced systemic resistance (ISR) in Arabidopsis thaliana against Pseudomonas syringae pv. tomato DC3000 (Pst), leading to a restriction of pathogen growth and disease development. Examination of local and systemic genes indicated that GP15-1 did not modulate the expression of any of the tested defence-related marker genes involved in salicylic acid (SA), jasmonic acid (JA) and ethylene signalling pathways. Subsequent challenge of GP15-1-colonized plants with Pst bacterium primed Arabidopsis plants for enhanced activation of the JA-inducible Atvsp (vegetative storage protein) gene at a later stage of infection. To assess the contribution of different signalling pathways in GP15-1-elicited plant growth and ISR, Arabidopsis genotypes implicated in SA signalling expressing the nahG transgene (NahG) or carrying disruption in NPR1 (npr1), JA signalling (jar1) and ethylene signalling (ein2) were tested. The GP15-1-induced plant growth and ISR were fully compromised in an ein2 mutation. Root colonization assay revealed that the inability of the ein2 mutant to express GP15-1-induced plant growth and ISR was not associated with reduced root colonization by GP15-1. In conclusion, our results demonstrate the ethylene signalling pathway is involved in plant growth promotion and ISR elicitation by the PGPF P. viridicatum GP15-1 in Arabidopsis. These results provide evidence that ethylene signalling has a substantial role in plant growth and disease resistance.

  • analysis of volatile organic compounds emitted by plant growth Promoting Fungus phoma sp gs8 3 for growth promotion effects on tobacco
    Microbes and Environments, 2013
    Co-Authors: Hushna Ara Naznin, Minako Kimura, Mitsuo Miyazawa, Mitsuro Hyakumachi
    Abstract:

    We extracted volatile organic compounds (VOCs) emitted by a plant growth-Promoting Fungus (PGPF) Phoma sp. GS8-3 by gas chromatography and identified them by mass spectrometry. All of the identified compounds belonged to C4-C8 hydrocarbons. Volatiles varied in number and quantity by the culture period of the Fungus (in days). 2-Methyl-propanol and 3-methyl-butanol formed the main components of the volatile blends for all the culture periods of Fungus. Growth-Promoting effects of the identified synthetic compounds were analyzed individually and in blends using tobacco plants. We found that the mixture of volatiles extracted from 3-day-old culture showed significant growth promotion in tobacco in vitro. The volatile blend showed better growth promotion at lower than higher concentrations. Our results confirm the potential role of volatile organic compounds in the mechanism of growth enhancement by GS8-3.

  • induction of systemic resistance against cucumber mosaic virus by penicillium simplicissimum gp17 2 in arabidopsis and tobacco
    Plant Pathology, 2012
    Co-Authors: Mohsen Mohamed Elsharkawy, Hideki Takahashi, M Shimizu, Mitsuro Hyakumachi
    Abstract:

    The plant growth-Promoting Fungus, Penicillium simplicissimum GP17-2, was evaluated for its ability to induce resistance against Cucumber mosaic virus (CMV) in Arabidopsis thaliana and tobacco plants. Treatment with barley grain inoculum (BGI) of GP17-2 significantly enhanced fresh weight, dry weight and leaf number of A. thaliana and tobacco plants 6 weeks after planting. Two weeks after CMV inoculation, all plants treated with BGI of GP17-2 or its culture filtrate (CF) showed a significant reduction in disease severity compared with non-treated control plants, which exhibited severe mosaic symptoms by the end of the experiment. The enzyme-linked immunosorbent assay (ELISA) demonstrated that CMV accumulation was significantly reduced in plants treated with GP17-2 or its CF relative to control plants. Based on RT-PCR, plants treated with GP17-2 (BGI or CF) also exhibited increased expression of regulatory and defence genes involved in the SA and JA/ET signalling pathways. These results suggested that multiple defence pathways in A. thaliana and tobacco were involved in GP17-2-mediated resistance to CMV, although neither the transgenic NahG line, nor the npr1, jar1 or ein3 mutants disrupted the response in A. thaliana. This is the first report to demonstrate the induction of systemic resistance against CMV by GP17-2 or its CF.

  • the plant growth Promoting Fungus fusarium equiseti and the arbuscular mycorrhizal Fungus glomus mosseae induce systemic resistance against cucumber mosaic virus in cucumber plants
    Plant and Soil, 2012
    Co-Authors: Mohsen Mohamed Elsharkawy, Hideki Takahashi, Masafumi Shimizu, Mitsuro Hyakumachi
    Abstract:

    Aims The aim of this study was to elucidate the effects of the interactions between the arbuscular mycorrhizal Fungus Glomus mosseae (Gm) and Fusarium equiseti GF18-3 on cucumber growth and the biocontrol of the yellow strain of Cucumber mosaic virus (CMV-Y).

  • talaromyces wortmannii fs2 emits β caryphyllene which promotes plant growth and induces resistance
    Journal of General Plant Pathology, 2011
    Co-Authors: Yasuo Yamagiwa, Mitsuro Hyakumachi, Yoshishige Inagaki, Yuki Ichinose, Kazuhiro Toyoda, Tomonori Shiraishi
    Abstract:

    A Plant-Growth-Promoting Fungus (PGPF), Talaromyces sp. was isolated from an agricultural field in southwestern Japan. We found that this Fungus emitted several terpenoid-like volatiles including β-caryophyllene. Then we investigated the effect of β-caryophyllene on Promoting the growth and inducing resistance of Brassica campestris L. var. perviridis. The compound significantly enhanced the growth of seedlings and their resistance to Colletotrichum higginsianum. On the basis of these results, we discuss the role of β-caryophyllene in the activities of PGPF.

Mohsen Mohamed Elsharkawy - One of the best experts on this subject based on the ideXlab platform.

  • induction of systemic resistance against cucumber mosaic virus by penicillium simplicissimum gp17 2 in arabidopsis and tobacco
    Plant Pathology, 2012
    Co-Authors: Mohsen Mohamed Elsharkawy, Hideki Takahashi, M Shimizu, Mitsuro Hyakumachi
    Abstract:

    The plant growth-Promoting Fungus, Penicillium simplicissimum GP17-2, was evaluated for its ability to induce resistance against Cucumber mosaic virus (CMV) in Arabidopsis thaliana and tobacco plants. Treatment with barley grain inoculum (BGI) of GP17-2 significantly enhanced fresh weight, dry weight and leaf number of A. thaliana and tobacco plants 6 weeks after planting. Two weeks after CMV inoculation, all plants treated with BGI of GP17-2 or its culture filtrate (CF) showed a significant reduction in disease severity compared with non-treated control plants, which exhibited severe mosaic symptoms by the end of the experiment. The enzyme-linked immunosorbent assay (ELISA) demonstrated that CMV accumulation was significantly reduced in plants treated with GP17-2 or its CF relative to control plants. Based on RT-PCR, plants treated with GP17-2 (BGI or CF) also exhibited increased expression of regulatory and defence genes involved in the SA and JA/ET signalling pathways. These results suggested that multiple defence pathways in A. thaliana and tobacco were involved in GP17-2-mediated resistance to CMV, although neither the transgenic NahG line, nor the npr1, jar1 or ein3 mutants disrupted the response in A. thaliana. This is the first report to demonstrate the induction of systemic resistance against CMV by GP17-2 or its CF.

  • the plant growth Promoting Fungus fusarium equiseti and the arbuscular mycorrhizal Fungus glomus mosseae induce systemic resistance against cucumber mosaic virus in cucumber plants
    Plant and Soil, 2012
    Co-Authors: Mohsen Mohamed Elsharkawy, Hideki Takahashi, Masafumi Shimizu, Mitsuro Hyakumachi
    Abstract:

    Aims The aim of this study was to elucidate the effects of the interactions between the arbuscular mycorrhizal Fungus Glomus mosseae (Gm) and Fusarium equiseti GF18-3 on cucumber growth and the biocontrol of the yellow strain of Cucumber mosaic virus (CMV-Y).

Hideki Takahashi - One of the best experts on this subject based on the ideXlab platform.

  • induction of systemic resistance against cucumber mosaic virus by penicillium simplicissimum gp17 2 in arabidopsis and tobacco
    Plant Pathology, 2012
    Co-Authors: Mohsen Mohamed Elsharkawy, Hideki Takahashi, M Shimizu, Mitsuro Hyakumachi
    Abstract:

    The plant growth-Promoting Fungus, Penicillium simplicissimum GP17-2, was evaluated for its ability to induce resistance against Cucumber mosaic virus (CMV) in Arabidopsis thaliana and tobacco plants. Treatment with barley grain inoculum (BGI) of GP17-2 significantly enhanced fresh weight, dry weight and leaf number of A. thaliana and tobacco plants 6 weeks after planting. Two weeks after CMV inoculation, all plants treated with BGI of GP17-2 or its culture filtrate (CF) showed a significant reduction in disease severity compared with non-treated control plants, which exhibited severe mosaic symptoms by the end of the experiment. The enzyme-linked immunosorbent assay (ELISA) demonstrated that CMV accumulation was significantly reduced in plants treated with GP17-2 or its CF relative to control plants. Based on RT-PCR, plants treated with GP17-2 (BGI or CF) also exhibited increased expression of regulatory and defence genes involved in the SA and JA/ET signalling pathways. These results suggested that multiple defence pathways in A. thaliana and tobacco were involved in GP17-2-mediated resistance to CMV, although neither the transgenic NahG line, nor the npr1, jar1 or ein3 mutants disrupted the response in A. thaliana. This is the first report to demonstrate the induction of systemic resistance against CMV by GP17-2 or its CF.

  • the plant growth Promoting Fungus fusarium equiseti and the arbuscular mycorrhizal Fungus glomus mosseae induce systemic resistance against cucumber mosaic virus in cucumber plants
    Plant and Soil, 2012
    Co-Authors: Mohsen Mohamed Elsharkawy, Hideki Takahashi, Masafumi Shimizu, Mitsuro Hyakumachi
    Abstract:

    Aims The aim of this study was to elucidate the effects of the interactions between the arbuscular mycorrhizal Fungus Glomus mosseae (Gm) and Fusarium equiseti GF18-3 on cucumber growth and the biocontrol of the yellow strain of Cucumber mosaic virus (CMV-Y).

Masafumi Shimizu - One of the best experts on this subject based on the ideXlab platform.

Ruchika Bajaj - One of the best experts on this subject based on the ideXlab platform.

  • protocol for biocontrol of soybean cyst nematode with root endophytic fungi
    2017
    Co-Authors: Ruchika Bajaj, Senyu Chen, Yinyin Huang, Ram Prasad, Ajit Varma, Vivek Kumar, Narendra Tuteja, Kathryn E Bushley
    Abstract:

    Plant-parasitic nematodes (PPNs) are major plant pests worldwide and cause severe economic and yield losses. The sedentary endoparasites, root-knot nematodes (RKNs, Meloidogyne spp.) and cyst nematodes (CNs, Globodera spp. and Heterodera spp.), are among the most economically damaging PPNs. Piriformospora indica, a plant growth-Promoting Fungus isolated from the Thar Deserts of western India, has been shown to protect a wide range of plants from various biotic and abiotic stresses. To evaluate the potential of P. indica to protect soybean (Glycine max) seedlings from damage by the soybean cyst nematode (SCN), we amended soil with two different concentrations of P. indica (2.5 % w/w, T1, and 5 % w/w, T2) and inoculated with second-stage juveniles (J2s) of SCN in each treatment. After 60 days, abundance of nematode eggs was measured by calculating SCN egg population densities. We found that egg density/100 cubic cm soil was significantly decreased by 29.7 % and 36.7 %, respectively, in the soil amended with T1 and T2 compared to a control. Amendment with P. indica also had a strong growth- and yield-Promoting effect in Soybean. Although root biomass was significantly decreased by 27.9 % and 33.5 % in the two treatments compared to the control, shoot biomass (dry weight) increased by 30.8 % and 8.2 % in the T1 and T2 treatments compared to the control. Additionally, plant development was accelerated and a 75 % increase in flowering was observed between the T1 treatment and the control. We conclude that P. indica used as a soil amendment decreases abundance of the SCN in soil and has plant growth-Promoting properties that may help counteract yield losses due to plant-parasitic nematodes.

  • the beneficial root endophyte piriformospora indica reduces egg density of the soybean cyst nematode
    Biological Control, 2015
    Co-Authors: Senyu Chen, Ruchika Bajaj, Yinyin Huang, Ram Prasad, Ajit Varma, Kathryn E Bushley
    Abstract:

    Abstract The soybean cyst nematode ( Heterodera glycines ) is a plant parasitic nematode that is a major plant pest worldwide and causes severe economic and yield losses. Piriformospora indica , a plant growth Promoting Fungus isolated from the Thar Deserts of western India, has been shown to protect a wide range of plants from various biotic and abiotic stresses. To evaluate the potential of P. indica to protect soybean ( Glycine max ) seedlings from damage by the soybean cyst nematode (SCN), we amended soil with two different concentrations of P. indica (2.5% and 5% w/w) and inoculated with second-stage juveniles (J2s) of SCN in each treatment. After 60 days, abundance of nematode eggs was measured by calculating SCN egg population densities. We found that egg density/100 cc soil was significantly decreased by 29.7% and 36.7% respectively in the soil amended with 2.5% and 5% P. indica compared to a control. Amendment with P. indica also had a strong growth and yield Promoting effect in Soybean. Although root biomass was significantly decreased by 27.9% and 33.5% in the two treatments compared to the control, shoot biomass (dry weight) increased by 30.8% and 8.2% in the 2.5% and 5% P. indica treatments compared to the control. Additionally, plant development was accelerated and a 75% increase in flowering was observed between the 2.5% P. indica treatment and the control. We conclude that P. indica used as a soil amendment decreases abundance of the SCN in soil and has Plant-Growth Promoting properties that may help offset yield losses due to plant parasitic nematodes.